Non-toxic tricyclic safe aquaculture method
By using disinfectants combined with water dragon king and sodium chloride in aquaculture, the breeding environment and aquatic seedlings were disinfected, and the problem of drug residues and pathogenic bacteria in traditional methods was solved, and a safe, irritating and residue-free breeding effect was achieved.
Patent Information
- Application Number
- CN202510015423.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-06
- Publication Date
- 2025-03-28
AI Technical Summary
Existing aquaculture disinfection methods are prone to cause drug residues in ponds, leading to stress responses in aquatic animals, and chlorine-based disinfectants cannot completely inactivate the highly pathogenic bacteria and cryptosporidium of fish and shrimp.
The non-toxic three-ring safe aquaculture method is used, and disinfectants combined with sodium chloride are used to disinfect the aquaculture empty ponds, whole ponds, daily supplementary water and aquaculture seedlings.
It has achieved thorough disinfection, reduced the harm of pathogenic bacteria and insect eggs, avoided drug residues and stress responses, and formed a safe, irritant and residue-free green breeding system.
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Abstract
Description
Technical Field
[0001] The invention belongs to aquaculture, and in particular relates to a non-toxic three-ring safe aquaculture method. Background Art
[0002] Pathogens such as bacteria, fungi, viruses, and parasites multiply in aquaculture water, infect and invade fish, and cause a large number of diseases. Regarding the problem of pathogens breeding in aquaculture water, the main principle is to prevent them before they are diseased, and prevention is more important than treatment. The reason for the serious aquatic epidemics is still that the growth environment is too bad, resulting in poor physical fitness of aquatic animals, and pathogens are easily bred in the environment, leading to diseases. Therefore, the essence of prevention and control in aquaculture is to improve the environment in which animals grow.
[0003] At present, the traditional method of aquaculture disinfection is to use lime alkali to disinfect ponds and chlorine-based disinfectants to disinfect aquaculture water bodies. The disinfectant prepared with lime alkali needs to reach a pH value greater than 12 to achieve partial disinfection purposes, but this disinfection method is prone to cause drug residues in the pond and cause emergency reactions in aquatic animals. At the same time, chlorine-based disinfectants cannot completely inactivate highly pathogenic bacteria and Cryptosporidium in fish and shrimp due to their relatively single active ingredients. Summary of the invention
[0004] In view of this, the object of the present invention is to provide a non-toxic three-ring safe aquaculture method, which can achieve thorough disinfection without inducing stress response, and form a safe, non-irritating, and residue-free green aquaculture system.
[0005] The present invention provides a non-toxic three-ring aquaculture method, comprising the following steps:
[0006] The empty breeding pond and the surrounding environment are disinfected with a first disinfectant; the first disinfectant includes Water Dragon King No. 5 and sodium chloride;
[0007] The whole pond water and daily supplementary water are disinfected with a second disinfectant, wherein the second disinfectant includes Water Dragon King No. 3 and sodium chloride;
[0008] Aquaculture seedlings are disinfected with a third disinfectant; the third disinfectant includes Water Dragon King No. 1;
[0009] The disinfected whole pond water is put into the disinfected empty breeding pond, and the disinfected aquaculture seedlings are put in for breeding management, and the water quality is monitored during the process.
[0010] Preferably, the first disinfectant comprises an aqueous solution containing 1% to 2% by mass of Water Dragon King No. 5 and 0.06% to 0.2% by mass of sodium chloride.
[0011] Preferably, the disinfection dosage of the first disinfectant is 120-150 mL / m 2; The disinfection time of the first disinfectant is 4 to 12 hours.
[0012] Preferably, the first disinfectant is used in combination with lime alkali for disinfection.
[0013] Preferably, the disinfection method is to prepare lime alkali into an aqueous solution with a pH of not less than 12 and then spray it for 2 to 3 days, and then disinfect with a first disinfectant;
[0014] The dosage of the lime alkali is 90-110 kg / mu;
[0015] The disinfection dosage of the first disinfectant is 60-80 mL / m 2 ; The disinfection time of the first disinfectant is 4 to 12 hours.
[0016] Preferably, the working mass concentration of Water Dragon King No. 3 in the second disinfectant is 3-5 ppm; the working mass concentration of sodium chloride is 0.18%-0.5%;
[0017] The treatment time of the second disinfectant includes 1 to 2 days.
[0018] Preferably, the working mass concentration of Water Dragon King No. 1 in the third disinfectant is 0.5 to 2 ppm;
[0019] The treatment time of the third disinfectant includes 1 to 2 days.
[0020] Preferably, during the breeding management period, starting from the time when the aquaculture seedlings are put into disinfection, the fourth disinfectant is used for disinfection every 5 to 7 days, and then the working concentration of the disinfectant is increased by 0.05 ppm every week;
[0021] The working concentration of the fourth disinfectant is 0.2-0.5 ppm.
[0022] Preferably, the types of the empty breeding ponds include earthen ponds for breeding shrimps, film-covered ponds or breeding ponds for factory-breeding shrimps and breeding ponds for breeding fish.
[0023] Preferably, according to the type of empty aquaculture pond, different suitable disinfectants are selected for disinfection during aquaculture management:
[0024] When the empty breeding pond is a soil pond for breeding shrimp, the fourth disinfectant includes Water Dragon King No. 1;
[0025] When the empty breeding pond is a film-covered pond or a breeding pond for factory-bred shrimp, the fourth disinfectant includes Water Dragon King No. 2;
[0026] When the empty breeding pond is a breeding pond for breeding fish, the fourth disinfectant includes Water Dragon King No. 4;
[0027] The working concentration of the Water Dragon King No. 1 or Water Dragon King No. 2 includes 0.2 to 0.4 ppm;
[0028] The working concentration of the Water Dragon King No. 4 includes 0.4 to 0.5 ppm.
[0029] The present invention provides a non-toxic three-ring aquaculture method, comprising the following steps: the empty breeding pond and the surrounding environment are disinfected with a first disinfectant; the first disinfectant includes Water Dragon King No. 5 and sodium chloride; the whole pond water and daily supplementary water are disinfected with a second disinfectant, the second disinfectant includes Water Dragon King No. 3 and sodium chloride; the aquaculture seedlings are disinfected with a third disinfectant; the third disinfectant includes Water Dragon King No. 1; the disinfected whole pond water is put into the disinfected empty breeding pond, and the disinfected aquaculture seedlings are put into the disinfected empty breeding pond for breeding management, and the water quality is monitored during the period. The present invention adopts Water Dragon King and sodium chloride combined disinfection to increase the redox potential by more than 3 times, realizes the thorough disinfection of bacteria, fungi, viruses and other pathogenic bacteria and pathogenic insect eggs in the empty breeding pond and breeding water, reduces the infringement of the breeding environment on aquaculture, and thus greatly reduces the harm of pathogenic bacteria and insect eggs. At the same time, the combination of the water dragon king and the aquaculture water body salinity regulator sodium chloride can also (increase the highly active ingredients such as chlorine free radicals and chlorine oxygen free radicals) to decompose excess organic matter, keep the bottom state in good condition, reduce the toxic and harmful substances such as nitrite, ammonia nitrogen, hydrogen sulfide in the water body, and the organic oxygen consumption is also reduced, and the bottom dissolved oxygen increases, providing a suitable growth environment for aquaculture. In addition, the disinfectant used in the breeding method of the present invention does not contain iodine preparations and does not produce drug residues, so as to avoid aquaculture animals from producing stress reactions due to the stimulation of disinfectants. It can be seen that the breeding method provided by the present invention can achieve antiviral, antibacterial, anti-stress reactions, improve the immunity of aquaculture animals and promote the growth of aquaculture animals, and create "aquaculture animal safety cages", with safety, no stimulation, no residue, one dose of multiple effects, balanced water bodies, and ensure that the breeding effect of the whole process can be widely promoted in the aquaculture industry. DETAILED DESCRIPTION
[0030] The present invention provides a non-toxic three-ring aquaculture method, comprising the following steps:
[0031] The empty breeding pond and the surrounding environment are disinfected with a first disinfectant; the first disinfectant includes Water Dragon King No. 5 and sodium chloride;
[0032] The whole pond water and daily supplementary water are disinfected with a second disinfectant, wherein the second disinfectant includes Water Dragon King No. 3 and sodium chloride;
[0033] Aquaculture seedlings are disinfected with a third disinfectant; the third disinfectant includes Water Dragon King No. 1;
[0034] The disinfected whole pond water is put into the disinfected empty breeding pond, and the disinfected aquaculture seedlings are put in for breeding management, and the water quality is monitored during the process.
[0035] The present invention disinfects the empty breeding pond and the surrounding environment with a first disinfectant; the first disinfectant comprises Water Dragon King No. 5 and sodium chloride.
[0036] In the present invention, the types of the empty breeding ponds preferably include earthen ponds for breeding shrimp, breeding ponds for breeding shrimp in simple sheds or factories, and breeding ponds for breeding fish. The first disinfectant includes an aqueous solution containing 1% to 2% by mass of Water Dragon King No. 5 and 0.06% to 0.2% by mass of sodium chloride. The Water Dragon King No. 5 was purchased from Zhongke Yunfeng Agricultural Technology (Chengdu) Co., Ltd. The mass percentage of the Water Dragon King No. 5 can be 1.2% to 1.8%, and can be 1.4%, 1.5%, 1.6%, and 1.7%. The mass percentage of the sodium chloride is preferably an aqueous solution of 0.01% to 0.18%, which can be 0.015% to 0.15%, 0.02% to 0.12%, or 0.05% to 0.01%. The disinfection dosage of the first disinfectant is preferably 120 to 150 mL / m 2 , can be 130~140mL / m 2 . The disinfection time of the first disinfectant is 4 to 12 hours, 5 to 11 hours, 6 to 10 hours, and 7 to 8 hours. The combined use of Water Dragon King No. 5 and sodium chloride can increase the redox potential by more than 3 times, thoroughly disinfect pathogenic pathogens and insect eggs such as bacteria, fungi, viruses, and completely destroy their living environment, so that the number and harm of later insects are greatly reduced; at the same time, it can also decompose excess organic matter, keep the bottom in good condition, reduce toxic and harmful substances such as nitrite, ammonia nitrogen, and hydrogen sulfide in the water body, and the organic oxygen consumption will also decrease, and the dissolved oxygen at the bottom will increase. There will be residual drug poisoning in the breeding pond after disinfection. If the drug toxicity is not completely eliminated after disinfection, it will cause poisoning and death of the breeding seedlings, causing heavy losses. In order to speed up the decomposition of pond cleaning drugs, the bottom of the pond can be turned over 1-2 times to quickly decompose the ingredients of the pond cleaning drugs.
[0037] In the present invention, the first disinfectant is preferably combined with lime alkali for disinfection. The disinfection method is to prepare lime alkali into an aqueous solution with a pH of not less than 12 and then spray it for 2 to 3 days, and then disinfect with the first disinfectant. The dosage of the lime alkali is preferably 90 to 110 kg / mu, and can be 100 kg / mu. The disinfection dosage of the first disinfectant is 60 to 80 mL / m 2 , can be 70mL / m 2 The disinfection time of the first disinfectant is 4 to 12 hours, can be 5 to 11 hours, can be 6 to 10 hours, can be 7 to 9 hours, and can be 8 hours.
[0038] The present invention disinfects the whole pond water and daily supplementary water with a second disinfectant, wherein the second disinfectant comprises Water Dragon King No. 3 and sodium chloride.
[0039] In the present invention, the working mass concentration of Water Dragon King No. 3 in the second disinfectant is preferably 3 to 5 ppm, and can be 4 ppm. The working mass concentration of the sodium chloride is preferably 0.18% to 0.5%, can be 0.2% to 0.4%, can also be 0.25% to 0.35%, and can also be 0.3%. The treatment time of the second disinfectant preferably includes 1 to 2 days. Water Dragon King No. 3 was purchased from Zhongke Yunfeng Agricultural Technology (Chengdu) Co., Ltd. The type of the whole pond water or daily supplementary water preferably includes at least one of the following: groundwater, spring water, river water and seawater. The types of the chlorine preparations preferably include sodium chloride, sodium hypochlorite and chlorine bleaching powder. After disinfection, the water quality of the whole pond water and daily supplementary water is measured, and when the highly pathogenic Vibrio is negative, it is put into the empty breeding pond.
[0040] The present invention disinfects aquaculture seedlings with a third disinfectant; the third disinfectant includes Water Dragon King No. 1.
[0041] In the present invention, the Water Dragon King No. 1 is purchased from Zhongke Yunfeng Agricultural Technology (Chengdu) Co., Ltd. The working mass concentration of Water Dragon King No. 1 in the third disinfectant is preferably 0.5-2ppm, 0.6-1.8ppm, 0.8-1.5ppm, or 1-1.2ppm. The treatment time of the third disinfectant preferably includes 1-2d, or 1.5d. Before the disinfected aquaculture seedlings are put into the breeding pond, a small amount of fry should be put in for trial breeding for 2-3 days, and the fish can be released in batches only after safety is determined.
[0042] The present invention uses the disinfected whole pond water to be put into the disinfected empty breeding pond, and the disinfected aquaculture seedlings are put into the pond for breeding management, and the water quality is monitored during the breeding management.
[0043] In the present invention, during the period of aquaculture management, starting from the aquaculture seedlings put into disinfection, the fourth disinfectant is used for disinfection every 5 to 7 days, and then the working concentration of the disinfectant is increased by 0.05ppm every week. It is preferred to disinfect once every 6 days. The working concentration of the fourth disinfectant is preferably 0.2 to 0.5ppm, and can be 0.4ppm. Different fourth disinfectants are selected for disinfection during aquaculture management according to the type of empty aquaculture pond: when the empty aquaculture pond is a soil pond for shrimp farming, the fourth disinfectant preferably includes Water Dragon King No. 1; when the empty aquaculture pond is a simple shed or a factory shrimp farming pond, the fourth disinfectant preferably includes Water Dragon King No. 2; when the empty aquaculture pond is a fish farming pond, the fourth disinfectant preferably includes Water Dragon King No. 4; the working concentration of Water Dragon King No. 1 or Water Dragon King No. 2 preferably includes 0.2 to 0.4ppm; the working concentration of Water Dragon King No. 4 preferably includes 0.4 to 0.5ppm. The Water Dragon King No. 2 was purchased from Zhongke Yunfeng Agricultural Technology (Chengdu) Co., Ltd. The Water Dragon King No. 4 was purchased from Zhongke Yunfeng Agricultural Technology (Chengdu) Co., Ltd. The disinfection method is preferably to dissolve in water and then spray or directly spray the powder of the disinfectant.
[0044] In the present invention, after the first disinfection, the working concentration of the disinfectant is increased by 0.05ppm every week thereafter, and the final working concentrations are 0.3ppm, 0.35ppm, 0.4ppm, 0.45ppm, 0.5ppm, 0.55ppm, 0.6ppm, 0.65ppm, and 0.7ppm.
[0045] In the present invention, depending on the weather, environmental changes, and specific conditions of breeding, it is mixed with feed and thrown into the feeding area to improve the feeding vitality and food intake of breeding animals. The dosage is preferably 50-100g / mu / m, 60-90g / mu / m, or 70-80g / mu / m. If the density of fish and shrimp is large or the water quality is abnormal, resulting in a large number of fish and shrimp floating on the pond, the dosage of the corresponding type of Water Dragon King product should be increased by 1-2 times depending on the situation. During the breeding period, it is also necessary to detect Cl - When the nitrite content reaches 0.2mg / l or above, increase the dosage of the corresponding Dashuilongwang product by 0.5-1 times, and adjust the Cl - The concentration is 5 to 6 times that of nitrite.
[0046] In the present invention, when other auxiliary water cultivation measures such as bacteria, acid, salt, etc. are used in combination, it is preferred to carry out the corresponding measures 12 hours after disinfection.
[0047] In the present invention, during the breeding period, every ten days or before a sudden weather change or a rainstorm, the 20-50 meter range around the breeding pond is thoroughly disinfected once with an aqueous solution containing 1% "Shui Long Wang" No. 5 and 6-10% sodium chloride at the concentration of "Shui Long Wang" No. 5. The specific dosage is preferably 10-20 ml / cm 2 .
[0048] In the present invention, the breeding method is realized based on the disinfection of the water dragon king regulator. The whole series of water dragon king regulator products contain a variety of relatively mild composite mediators, see Table 1. After the composite mediator completes its mediation mission, it is designed as a supplement for the necessary trace elements in aquaculture water. The free radical composite mediator can fully mediate the peroxy bridge complex to produce reactive oxygen species. After the reactive oxygen species release highly active energy to disinfect pathogenic bacteria and mineralize organic toxic phases, relatively fine oxygen bubbles are formed, which can be well integrated with the water body; the photosynthetic composite mediator can not only increase the amount of reactive oxygen species generated, but also increase the release rate of the water dragon king under high temperature and low pressure, while changing the surface tension of the water body, establishing an effective channel for the fusion of water and oxygen, and allowing more oxygen in the air to enter the water body under the action of wind or aerators; the bionic singlet oxygen composite mediator forms a large number of ecological nano singlet oxygen microbubbles, which are retained on the oxygen bridge of the hydrated cell body for a long time (more than half an hour), forming a tiny bubble gas floating body carrying relaxation energy (biophoton energy), which is like the singlet oxygen water mist in the forest, and is spread throughout the water body to protect the water body. After aquaculture animals absorb energy, they improve immunity, reduce mortality, increase protein safety and quality, and increase production capacity by 10% to 20%. The various active substances formulated in the product system, as well as small molecule organic acids, osmotic pressure regulators, etc., which are generated by the mineralization of organic macromolecules by highly active free radical clusters, can increase the solubility of oxygen in water, dissolve more oxygen in water, and form oxygen bubbles floating on the water surface or adsorbed on other attachments. When the dissolved oxygen in the water is slightly reduced or the water pressure changes slightly, the oxygen bubbles will immediately burst under the action of osmotic pressure, and release oxygen into the water at the same time, thereby improving the utilization rate of oxygen. Oxygen bubbles and singlet oxygen microbubbles can keep the pond water in a high dissolved oxygen state for a long time.
[0049] Table 1 Ion phases contained in the series of Water Dragon King regulator products (Water Dragon King No. 1 to Water Dragon King No. 5)
[0050] Content of each ion in 100 mg of Water Dragon King (mg) 1# 2# 3# 4# 5# <![CDATA[K + ]]> 14.6 14.0 15.4 14.9 16.2 <![CDATA[Na + ]]> 8.9 8.9 8.9 8.9 8.9 <![CDATA[Ca 2+ ]]> 1.2 1.8 0 1.5 0 <![CDATA[Mg 2+ ]]> 2.4 2.0 2.8 3.0 2.8 <![CDATA[SO4 2- ]]> 38.5 37.9 40.2 37.6 39.9 <![CDATA[Cl - ]]> 1.5 1.5 1.5 1.5 1.0 <![CDATA[Sr 2+ ]]> 0.2 0.2 0.4 0.3 0.4 <![CDATA[HCOO - ]]> 1.6 1.2 0 2.0 0
[0051] The active substances in the series of Shuilongwang regulator products have ionization effects, which can interact with ions in the aquaculture water, balance the pH value of the water, and improve the water's own buffering capacity, thus having the function of repairing the water. Its mechanism of action is: when the Shuilongwang electrolyte reacts with the water, the cations (hydrogen ions) in the water produced are weak acids; the anions (hydroxyl ions) in the water produced are weak bases. It is well known that mixed solutions composed of weak acids and their salts, weak bases and their salts can offset, reduce (or add) the effects of strong acids or strong bases on the pH of water (or solutions) to a certain extent, thereby keeping the pH value of the water (or solution) relatively stable. Shuilongwang products have the function of balancing the pH of water.
[0052] The series of Water Dragon King regulator products are rich in water ion buffers and water-soluble mineral microelements, and have strong comprehensive buffering capacity. They can not only stabilize the pH value within a suitable range, but also effectively supplement and regulate the mineral microelements in the water. Maintaining the content of mineral microelements in the water at a certain concentration is conducive to enhancing the stability of the water.
[0053] The following is a detailed description of a non-toxic tricyclic aquaculture method provided by the present invention in conjunction with the embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0054] Example 1
[0055] A non-toxic three-ring aquaculture method
[0056] This embodiment takes a shrimp farming pond (earth pond) as an example to illustrate the farming method.
[0057] 1. Pond cleaning and cleaning treatment
[0058] The empty breeding pond and the environment within 50 meters around the pond are cleaned of sludge, impurities and thoroughly disinfected. The disinfection method is to disinfect the bottom, wall and ground within 50 meters around the pond with the first disinfectant; the first disinfectant is an aqueous solution containing 2% mass percentage concentration of Water Dragon King No. 5 and 0.2% sodium chloride, and the spraying dosage of the first disinfectant is 120mL / m 2 ,, the disinfection time is 4h.
[0059] 2. Disinfection of pond water and seedling water
[0060] 1) Disinfection of pond water preparation and daily supplementary water before stocking shrimp ponds
[0061] Groundwater was used as the source of water for the whole pond, and No. 2 disinfectant was used for disinfection. No. 2 disinfectant contained Water Dragon King (regulator) No. 3 and sodium chloride; the working concentration of Water Dragon King (regulator) No. 3 was 3ppm, the working concentration of the sodium chloride was 0.3ppm, and the disinfection time was 1d. The water quality was measured after disinfection.
[0062] 2) Disinfection of shrimp fry water
[0063] Before stocking the shrimp fry, soak and disinfect the shrimp fry with No. 3 disinfectant. No. 3 disinfectant is Water Dragon King No. 1 with a working concentration of 2ppm. The soaking and disinfection time lasts for 1 day.
[0064] 3. Breeding control
[0065] After the shrimp fry are put into the breeding pond, the water quality is scientifically tested. Every week, a working concentration of 0.3ppm of Water Dragon King No. 1 is added for disinfection. As the weekly feed feeding amount increases, 0.05ppm of Water Dragon King No. 1 disinfectant is added each week. Depending on the weather, environmental changes, and specific breeding conditions, it can be mixed with the feed and sprinkled in the feeding area to increase the shrimp's feeding activity and food intake. The dosage is 50-100g / mu / meter. When the density of fish and shrimp is too high or the water quality is abnormal (such as: Vibrio exceeds the cube of ten; ammonia nitrogen, nitrite, and hydrogen sulfide concentrations exceed the standard), resulting in a large number of fish and shrimp floating and flooding the pond, the dosage of Water Dragon King should be increased by 1-2 times depending on the situation. At the same time, Cl is tested throughout the process - and nitrite content, observe the multiple relationship between the two. When the nitrite concentration is higher than 0.2 mg / L, increase the dosage of Water Dragon King No. 1 by 0.5 to 1 times, and adjust the Cl - The concentration is 5 to 6 times that of nitrite.
[0066] When the weather changes suddenly or before a rainstorm or in clear weather, the breeding pond and the area within 50 meters around the pond should be thoroughly disinfected once every ten days with the fourth disinfectant (working concentration 1% "Water Dragon King" No. 5 and working concentration 0.1% sodium chloride aqueous solution), the dosage is 20ml / m 2 .
[0067] Comparative Example 1
[0068] A traditional aquaculture method
[0069] This comparative example uses a shrimp pond (earth pond) as an example to illustrate the breeding method
[0070] 1. Pond cleaning and cleaning treatment
[0071] The empty pond and the environment within 50m around the pond should be cleaned of sludge, impurities and thoroughly disinfected. The disinfection method is to dry-sprinkle the pond with a lime-alkali aqueous solution with a pH value of 13, with a dosage of 120kg / mu for 2 days.
[0072] 2. Disinfection of pond water and seedling water
[0073] 1) Disinfection of pond water preparation and daily supplementary water before stocking shrimp ponds
[0074] Groundwater is used as the source of water for the whole pond, and sodium hypochlorite disinfectant is used for disinfection. The working concentration of sodium hypochlorite disinfectant is 20ppm, and the disinfection time is 1 day. The water quality is measured after disinfection.
[0075] 2) Disinfection of shrimp fry water
[0076] Before releasing the shrimp fry, soak and disinfect them with iodine disinfectant at a working concentration of 10ppm, and the soaking and disinfection time should last for 0.5-2 hours.
[0077] 3. Breeding control
[0078] After the shrimp fry are put into the breeding pond, the water quality is scientifically tested. Every week, a working concentration of 0.3ppm sodium hypochlorite disinfectant is added to disinfect the breeding water. As the weekly feed feeding amount increases, the working concentration of 0.2ppm disinfectant is added each week. Depending on the weather, environmental changes, and specific breeding conditions, it can be mixed with the feed and sprinkled in the feeding area to increase the shrimp's feeding vitality and food intake. The dosage is 50-100g / mu / m. When the density of fish and shrimp is too high or the water quality is abnormal, resulting in a large number of fish and shrimp floating and flooding the pond, 1-2 times the amount of sodium hypochlorite should be added depending on the situation.
[0079] When the weather changes suddenly or before a rainstorm or in clear weather, the breeding pond and the area within 50 meters around it should be thoroughly disinfected once every ten days using sodium hypochlorite disinfectant at a dosage of 20ppm.
[0080] The culture method of Example 1 and the traditional aquaculture method of Comparative Example 1 were used to culture whiteleg shrimp in a pond in Zhongshan, Guangzhou (5 mu each). The culture effect comparison is shown in Table 2.
[0081] Table 2 Comparison of the effects of the aquaculture method of the present invention (Example 1) and the traditional aquaculture method (Comparative Example 1) on the aquaculture of South American shrimp
[0082]
[0083]
[0084] It can be seen from the data in Table 2 that the aquaculture method carried out in Example 1 of the present invention has outstanding advantages in terms of both water quality and the yield of Penaeus vannamei.
[0085] Example 2
[0086] A non-toxic three-ring aquaculture method
[0087] This embodiment takes a shrimp farming pond (factory) as an example to illustrate the farming method.
[0088] 1. Pond cleaning and cleaning treatment
[0089] Clean the sludge, remove impurities and thoroughly disinfect the empty aquaculture pond and the environment within 50 meters around the pond. The disinfection method is to first disinfect the pond bottom, pond wall and the ground within 50 meters around the pond with lime alkali, spray a lime alkali aqueous solution with a pH of 13, and the dosage of lime alkali is 100kg / mu. After 2 days of lime alkali disinfection, disinfect with the first disinfectant. The first disinfectant is an aqueous solution containing 1.5% of the mass percentage concentration of Water Dragon King No. 5 and 0.15% of sodium chloride. The spraying dosage of the first disinfectant is 60mL / m 2 ,, the disinfection time is 8h.
[0090] 2. Disinfection of pond water and seedling water
[0091] 1) Disinfection of pond water preparation and daily supplementary water before stocking shrimp ponds
[0092] The river water was used as the source of the pond water, and No. 2 disinfectant was used for disinfection. No. 2 disinfectant contained Water Dragon King (regulator) No. 3 and sodium chloride; the working concentration of Water Dragon King (regulator) No. 3 was 5ppm, the working concentration of the sodium chloride was 0.5ppm, and the disinfection time was 1 day. The water quality was measured after disinfection.
[0093] 2) Disinfection of shrimp fry water
[0094] Before stocking the shrimp fry, the shrimp fry were soaked and disinfected with No. 3 disinfectant. No. 3 disinfectant was Water Dragon King No. 1 with a working concentration of 1 ppm. The soaking and disinfection time lasted for 1.5 days.
[0095] 3. Breeding control
[0096] After the shrimp fry are put into the breeding pond, the water quality is scientifically tested. Every week, add 0.3ppm of Water Dragon King No. 2 for disinfection. As the weekly feed intake increases, add 0.05ppm of Water Dragon King No. 2 disinfectant each week. Depending on the weather, environmental changes, and specific breeding conditions, it can be mixed with the feed and sprinkled in the feeding area to increase the shrimp's feeding activity and food intake. The dosage is 50-100g / mu / m. When the fish and shrimp density is too high or the water quality is abnormal (such as: Vibrio exceeds 10 3 CFU / ml; when the concentration of ammonia nitrogen, nitrite, and hydrogen sulfide exceeds the standard, causing fish and shrimp to float and flood the pond, the dosage of Water Dragon King should be increased by 1 to 2 times depending on the situation. At the same time, Cl - and nitrite content, observe the multiple relationship between the two. When the nitrite concentration is higher than 0.2 mg / L, increase the dosage of Water Dragon King No. 1 by 0.5 to 1 times, and adjust the Cl - The concentration is 5 to 6 times that of nitrite.
[0097] When the weather changes suddenly or before a rainstorm or in clear weather, the breeding pond and the area within 50 meters around the pond should be thoroughly disinfected once every ten days with the fourth disinfectant (working concentration 1% "Water Dragon King" No. 5 and working concentration 0.1% sodium chloride aqueous solution), the dosage is 20ml / m 2 .
[0098] Example 2 Breeding method and comparative example 1 The traditional aquaculture method was used to culture white shrimp in an industrial pond in Zhanjiang, Guangzhou (200 cubic meters each). The comparison of the breeding effects is shown in Table 3.
[0099] Table 3 Comparison of the effects of the aquaculture method of the present invention (Example 2) and the traditional aquaculture method (Comparative Example 1) on the aquaculture of South American shrimp
[0100]
[0101] It can be seen from the data in Table 3 that the aquaculture method carried out in Example 1 of the present invention has outstanding advantages in terms of both water quality and the yield of Penaeus vannamei.
[0102] Example 3
[0103] A non-toxic three-ring aquaculture method
[0104] This embodiment takes a fish farming pond (factory farming pond) as an example to illustrate the farming method.
[0105] 1. Pond cleaning and cleaning treatment
[0106] The empty breeding pond and the environment within 50 meters around the pond are cleaned of sludge, impurities and thoroughly disinfected. The disinfection method is to disinfect the bottom, wall and ground within 50 meters around the pond with the first disinfectant; the first disinfectant is an aqueous solution containing 1% mass percentage concentration of Water Dragon King No. 5 and 0.1% sodium chloride, and the spraying dosage of the first disinfectant is 150mL / m 2 ,, the disinfection time is 12h.
[0107] 2. Disinfection of pond water and seedling water
[0108] 1) Disinfection of pond water preparation and daily supplementary water before stocking shrimp ponds
[0109] Seawater was used as the source of water for the whole pond, and No. 2 disinfectant was used for disinfection. No. 2 disinfectant contained Water Dragon King (regulator) No. 3 and sodium chloride; the working concentration of Water Dragon King (regulator) No. 3 was 4 ppm, the working concentration of the sodium chloride was 0.4 ppm, and the disinfection time was 1 day. The water quality was measured after disinfection.
[0110] 2) Disinfection of shrimp fry water
[0111] Before stocking the shrimp fry, soak and disinfect the shrimp fry with No. 3 disinfectant. No. 3 disinfectant is Water Dragon King No. 1 with a working concentration of 0.5ppm. The soaking and disinfection time lasts for 2 days.
[0112] 3. Breeding control
[0113] After the shrimp fry are put into the breeding pond, the water quality is scientifically tested. Every week, a working concentration of 0.5ppm of Water Dragon King No. 4 is added for disinfection. As the weekly feed feeding amount increases, 0.05ppm of Water Dragon King No. 4 disinfectant is added each week. Depending on the weather, environmental changes, and specific breeding conditions, it can be mixed with the feed and sprinkled in the feeding area to increase the shrimp's feeding activity and food intake. The dosage is 50-100g / mu / meter. When the density of fish and shrimp is too high or the water quality is abnormal (such as: Vibrio exceeds the cube of ten; ammonia nitrogen, nitrite, and hydrogen sulfide concentrations exceed the standard), resulting in a large number of fish and shrimp floating and flooding the pond, the dosage of Water Dragon King should be increased by 1-2 times depending on the situation. At the same time, Cl is tested throughout the process - and nitrite content, observe the multiple relationship between the two. When the nitrite concentration is higher than 0.2 mg / L, increase the dosage of Water Dragon King No. 1 by 0.5 to 1 times, and use sodium chloride to adjust the Cl- concentration to 5 to 6 times that of nitrite.
[0114] When the weather changes suddenly or before a rainstorm or in clear weather, the breeding pond and the area within 50 meters around the pond should be thoroughly disinfected once every ten days with the fourth disinfectant (working concentration 1% "Water Dragon King" No. 5 and working concentration 0.1% sodium chloride aqueous solution), the dosage is 20ml / m 2 .
[0115] Example 3 Breeding method and comparative example 1 The traditional aquaculture method was used to culture whiteleg shrimp in a simple shed in Jiangsu (100 cubic meters each), and the breeding effect comparison is shown in Table 4.
[0116] Table 4 Comparison of the effects of the aquaculture method of the present invention (Example 3) and the traditional aquaculture method (Comparative Example 1) on the aquaculture of South American shrimp
[0117]
[0118] It can be seen from the data in Table 4 that the aquaculture method carried out in Example 1 of the present invention has outstanding advantages in terms of both water quality and the yield of Penaeus vannamei.
[0119] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A non-toxic three-ring aquaculture method, characterized in that: The following steps are involved: The empty breeding pond and the surrounding environment are disinfected with a first disinfectant; the first disinfectant includes Water Dragon King No. 5 and sodium chloride; The whole pond water and daily supplementary water are disinfected with a second disinfectant, wherein the second disinfectant includes Water Dragon King No. 3 and sodium chloride; Aquaculture seedlings are disinfected with a third disinfectant; the third disinfectant includes Water Dragon King No. 1; The disinfected whole pond water is put into the disinfected empty breeding pond, and the disinfected aquaculture seedlings are put in for breeding management, and the water quality is monitored during the process.
2. The non-toxic three-ring aquaculture method according to claim 1, characterized in that: The first disinfectant comprises an aqueous solution containing 1% to 2% by mass of Water Dragon King No. 5 and 0.06% to 0.2% by mass of sodium chloride.
3. The non-toxic three-ring aquaculture method according to claim 2, characterized in that: The disinfection dosage of the first disinfectant is 120-150 mL / m 2 ; The disinfection time of the first disinfectant is 4 to 12 hours.
4. The non-toxic three-ring aquaculture method according to claim 2, characterized in that: The first disinfectant is combined with lime alkali for disinfection.
5. The non-toxic three-ring aquaculture method according to claim 4, characterized in that: The disinfection method comprises preparing lime alkali into an aqueous solution with a pH value not less than 12, spraying the solution for 2 to 3 days, and then disinfecting the solution with a first disinfectant; The dosage of the lime alkali is 90-110 kg / mu; The disinfection dosage of the first disinfectant is 60-80 mL / m 2 ; The disinfection time of the first disinfectant is 4 to 12 hours.
6. The non-toxic three-ring aquaculture method according to claim 1, characterized in that: The working mass concentration of Water Dragon King No. 3 in the second disinfectant is 3-5 ppm; the working mass concentration of sodium chloride is 0.18%-0.5%; The treatment time of the second disinfectant includes 1 to 2 days.
7. The non-toxic three-ring aquaculture method according to claim 1, characterized in that: The working mass concentration of Water Dragon King No. 1 in the third disinfectant is 0.5 to 2 ppm; The treatment time of the third disinfectant includes 1 to 2 days.
8. The non-toxic three-ring aquaculture method according to claim 1, characterized in that: During the aquaculture management period, starting from the time the aquaculture seedlings are disinfected, the fourth disinfectant is used for disinfection every 5 to 7 days, and then the working concentration of the disinfectant is increased by 0.05 ppm every week; The working concentration of the fourth disinfectant is 0.2-0.5 ppm.
9. The non-toxic three-ring aquaculture method according to claim 1, characterized in that: The types of the empty breeding ponds include earthen ponds for breeding shrimps, film-covered ponds or breeding ponds for factory breeding shrimps and breeding ponds for breeding fishes.
10. The non-toxic three-ring aquaculture method according to claim 9, characterized in that: Depending on the type of empty pond, different fourth disinfectants are selected for disinfection during the breeding management period: When the empty breeding pond is a soil pond for breeding shrimp, the fourth disinfectant includes Water Dragon King No. 1; When the empty breeding pond is a film-covered pond or a breeding pond for factory-bred shrimp, the fourth disinfectant includes Water Dragon King No. 2; When the empty breeding pond is a breeding pond for breeding fish, the fourth disinfectant includes Water Dragon King No. 4; The working concentration of the Water Dragon King No. 1 or Water Dragon King No. 2 includes 0.2 to 0.4 ppm; The working concentration of the Water Dragon King No. 4 includes 0.4 to 0.5 ppm.
Citation Information
Patent Citations
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