Synergist capable of improving overwintering rate of alfalfa and planting method

Abstract

The invention discloses a synergist capable of improving the overwintering rate of alfalfa and a planting method, and relates to the technical field of agriculture, the synergist comprises the following raw materials: brassinolide, chitosan oligosaccharide coated riboflavin microcapsule powder, brown sugar and water in a mass ratio of (0.5-1.5): (1-3): (6-14): (800-1200); the planting method comprises the following steps: step 1, preparing an alfalfa field for crop rotation, cutting the overground part, integrally taking out root necks and branch buds, cutting the root necks and the branch buds of a whole plant into alfalfa bud particles with the diameter of 0.5-1cm through mechanical equipment, and spraying the alfalfa bud particles with a synergist; 2, the alfalfa bud particles subjected to spraying treatment are sown, the sowing depth ranges from 2 cm to 3 cm, the row spacing of a forage grass planting field ranges from 15 cm to 20 cm, the row spacing of a seed planting field ranges from 30 cm to 40 cm, and the sowing rate ranges from 100 kg / mu to 200 kg / mu. According to the synergist and the planting method provided by the invention, the cold resistance and stress resistance of alfalfa can be effectively improved, the overwintering rate is increased, and the utilization of an alfalfa bud bank is realized.

Description

Technical Field

[0001] The present application relates to the field of agricultural technology, and in particular to a synergist and a planting method for improving the wintering rate of alfalfa. Background Art

[0002] Alfalfa is a high-quality legume forage with high feeding value and rich nutrition. It is a favorite forage for various livestock, poultry and fish. At the same time, it has a long growth period, many underground nodules and a well-developed root system. It is also an excellent soil improvement and fertilization plant. Scientific planting can achieve higher economic benefits, and it also plays an important role in soil improvement, soil and water conservation, etc.

[0003] The conventional utilization cycle of alfalfa artificial grassland is usually maintained between 4 and 6 years, after which its yield will gradually enter a decreasing stage, indicating the decline of grassland productivity. At this time, it is particularly important to implement a reasonable rotation system. It is worth noting that alfalfa is a crop with strong asexual reproduction ability. After mowing, a large number of asexual reproductive bodies will accumulate in its root system and near the surface, which constitute a huge bud bank.

[0004] Unfortunately, in plots that are prepared for crop rotation, these potential asexual propagule bud banks are often neglected due to the lack of effective means of utilization, and eventually abandoned, resulting in a huge waste of biological resources; especially in cold regions and times, alfalfa sprouts have relatively weak cold resistance and generally low overwintering rates, further exacerbating the problem of biological resource waste. Due to the inability to effectively cope with the challenges of low temperature environments, a large number of alfalfa sprouts die in winter, resulting in a sharp decrease in the number of asexual propagules in the bud bank, which in turn affects the natural regeneration and long-term productivity of alfalfa grasslands. Therefore, exploring and developing efficient utilization strategies for alfalfa asexual propagule bud banks and improving the overwintering rate of alfalfa sprouts are of great significance for extending the service life of alfalfa grasslands and improving the comprehensive benefits of land resources. Summary of the invention

[0005] In order to improve the overwintering rate of alfalfa and realize the utilization of alfalfa sprout bank, the present application provides a synergist and a planting method that can improve the overwintering rate of alfalfa.

[0006] The present application provides a synergist capable of improving the wintering rate of alfalfa, which adopts the following technical scheme:

[0007] A synergist capable of improving the wintering rate of alfalfa comprises raw materials including brassinolide, chitosan oligosaccharide coated riboflavin microcapsule powder, brown sugar and water; the mass ratio of the brassinolide, chitosan oligosaccharide coated riboflavin microcapsule powder, brown sugar and water is 0.5-1.5:1-3:6-14:800-1200.

[0008] Preferably, the mass ratio of brassinolide, chitosan oligosaccharide-coated riboflavin microcapsule powder, brown sugar and water is 0.8-1.2:1.5-2.5:8-12:900-1100.

[0009] Preferably, the mass ratio of brassinolide, chitosan oligosaccharide-coated riboflavin microcapsule powder, brown sugar and water is 1:2:10:1000.

[0010] Preferably, the method for preparing the chitosan oligosaccharide coated riboflavin microcapsule powder comprises the following steps:

[0011] S1. Dissolving zinc nitrate hexahydrate in deionized water to prepare a zinc nitrate hexahydrate solution; dissolving 2-methylimidazole in methanol to prepare a 2-methylimidazole solution;

[0012] S2. Add 2-methylimidazole solution to zinc nitrate hexahydrate solution and stir for 5-10 minutes, add riboflavin and continue stirring for 10-20 minutes, centrifuge the resulting suspension at a speed of 10000-15000 rpm for 15-20 minutes, and then wash and centrifuge several times with deionized water and methanol alternately to obtain riboflavin microspheres;

[0013] S3. Place the riboflavin microspheres in the cationic chitosan oligosaccharide solution for coating for 30-40 minutes, wash with deionized water, and dry in a vacuum drying oven at 30-40°C for 24-30 hours to obtain chitosan oligosaccharide coated riboflavin microcapsule powder.

[0014] Preferably, the mass ratio of zinc nitrate hexahydrate, 2-methylimidazole and riboflavin is 10-12:22-26:1.

[0015] Preferably, the mass ratio of the deionized water to the zinc nitrate hexahydrate in S1 is 18-22:1.

[0016] Preferably, the mass ratio of methanol to 2-methylimidazole in S2 is 22-26:1.

[0017] Preferably, the concentration of the cationic chitosan oligosaccharide solution in S3 is 0.6-0.9%.

[0018] The present application also provides a planting method that can improve the wintering rate of alfalfa, using the following technical solution:

[0019] A planting method capable of improving the wintering rate of alfalfa, characterized in that it comprises the following steps:

[0020] Step 1: Prepare the alfalfa field for crop rotation, mow the above-ground part, take out the root neck and branch buds as a whole, cut the root neck and branch buds of the whole plant into alfalfa sprout particles with a diameter of 0.5-1cm through mechanical equipment, and spray the alfalfa sprout particles with a synergist;

[0021] Step 2: Sow the sprayed alfalfa sprout particles at a sowing depth of 2-3 cm, select a row spacing of 15-20 cm for forage fields, select a row spacing of 30-40 cm for seed fields, and a sowing amount of 100-200 kg / mu.

[0022] Preferably, the spraying treatment time in step 1 is 2-4 hours.

[0023] In summary, the present application includes at least one of the following beneficial technical effects:

[0024] 1. By adopting the above technical scheme, brassinolide is a highly oxygenated plant steroid hormone, which plays an important regulatory role in improving the resistance, quality and yield of alfalfa sprouts. Brassinolide can inhibit the leakage of plant electrolytes under chilling injury, enhance the activity of related enzymes, and increase the accumulation of proline by promoting the activity of certain synthases and transaminases and inhibiting the activity of dehydrogenases, thereby improving the cold resistance of plants; riboflavin can enhance the cold resistance of alfalfa sprouts. When alfalfa sprouts are subjected to low temperature stress, riboflavin may regulate the redox balance in the plant body and protect the stability of cell membranes and organelles, thereby reducing the damage of low temperature to plants; at the same time, riboflavin can also enhance the disease resistance of alfalfa sprouts to pathogens, and improve the defense ability of plants by inducing the expression of disease resistance-related genes in plants; brown sugar can be used as a carbon source for the growth of alfalfa sprouts to provide energy for growth. In terms of the cold resistance of alfalfa sprouts, sugar substances can be used as osmotic regulating substances to help plants maintain water balance in cells and prevent a large amount of water in the cells from leaking out and causing serious damage to plants. The present application compounds brassinolide, riboflavin and brown sugar as a synergist for improving the overwintering rate of alfalfa, effectively promoting the growth of alfalfa sprout particles, enhancing the cold resistance and stress resistance of the sprout particles, enhancing the overwintering rate, and realizing the utilization of the alfalfa sprout bank.

[0025] 2. The present application adopts ZIF-8 as a carrier to encapsulate riboflavin, which can improve the efficiency of the photosensitive redox reaction of riboflavin under specific lighting conditions. ZIF-8 is a metal organic framework (MOF) material with the characteristics of high specific surface area, high porosity, high stability and adjustable pore size, which helps to protect riboflavin from damage by environmental factors and can control the release rate of riboflavin to better adapt it to the growth needs of plants; chitosan oligosaccharide, as a natural bioactive substance, has good biocompatibility. After the surface of ZIF-8 encapsulated riboflavin microcapsules is coated with chitosan oligosaccharide, it is easier to be recognized and accepted by plant cells, thereby improving the utilization rate of riboflavin in plants; and chitosan oligosaccharide can regulate the hormone level in plants, promote the development of plant roots, and improve the cold resistance and stress resistance of plants. The coated ZIF-8 encapsulated riboflavin microcapsules can act more effectively on alfalfa sprouts, promoting the growth of alfalfa sprouts and nutrient absorption capacity.

[0026] 3. By adopting the above technical scheme, after the alfalfa sprout bank is granulated, a synergist consisting of brassinolide, chitosan oligosaccharide-coated riboflavin microcapsule powder, and brown sugar is used to spray the alfalfa sprout particles, which effectively improves the vitality of the sprout particles, promotes their rapid growth and cold resistance, and then uses the asexual propagules of the sprout particles treated with the synergist to build an alfalfa artificial grassland, thereby realizing the utilization and large-scale utilization of the alfalfa sprout bank. Compared with the construction of artificial grassland by seeds, the sowing of the sprout bank has better cold resistance and stress resistance, and the wintering rate is enhanced. DETAILED DESCRIPTION

[0027] The present application is further described in detail below with reference to the embodiments.

[0028] The chemical reagents used in the preparation examples, embodiments and comparative examples provided by the present invention are all commercially available products, and their brands and manufacturers are as follows:

[0029] Zinc nitrate hexahydrate, Hubei Rishengchang New Material Technology Co., Ltd.;

[0030] 2-Methylimidazole, Wuhan Zeyou Chemical Co., Ltd.;

[0031] Riboflavin, Shenzhen Lijing Biochemical Technology Co., Ltd.;

[0032] Cationic chitosan oligosaccharide, Shandong Yatu Biotechnology Co., Ltd.;

[0033] Brown sugar, Guangzhou Chengshun Trading Co., Ltd.;

[0034] Brassinolide, Hubei Hongxin Ruiyu Fine Chemical Co., Ltd.

[0035] Preparation Example

[0036] Preparation Example 1

[0037] S1. Dissolve 1 g of zinc nitrate hexahydrate in 18 g of deionized water (the mass ratio of deionized water to zinc nitrate hexahydrate in this embodiment is 18:1) to prepare a zinc nitrate hexahydrate solution; dissolve 2.2 g of 2-methylimidazole in 48.4 g of methanol (the mass ratio of methanol to 2-methylimidazole in this embodiment is 22:1) to prepare a 2-methylimidazole solution;

[0038] S2. Add the 2-methylimidazole solution to the zinc nitrate hexahydrate solution and stir at 300 rpm for 5 min, add 0.1 g of riboflavin (the mass ratio of zinc nitrate hexahydrate, 2-methylimidazole, and riboflavin in this embodiment is 10:22:1), continue stirring at 300 rpm for 10 min, centrifuge the resulting suspension at 10000 rpm for 15 min, and then wash and centrifuge alternately with deionized water and methanol for 3 times to obtain riboflavin microspheres;

[0039] S3. The riboflavin microspheres were placed in a 0.6% cationic chitosan oligosaccharide solution for coating for 30 minutes, washed with deionized water, and dried in a vacuum drying oven at 30°C for 24 hours to obtain chitosan oligosaccharide-coated riboflavin microcapsule powder.

[0040] Preparation Example 2

[0041] S1. Dissolve 1.1 g of zinc nitrate hexahydrate in 32.4 g of deionized water (the mass ratio of deionized water to zinc nitrate hexahydrate in this embodiment is 18:1) to prepare a zinc nitrate hexahydrate solution; dissolve 2.4 g of 2-methylimidazole in 52.8 g of methanol (the mass ratio of methanol to 2-methylimidazole in this embodiment is 22:1) to prepare a 2-methylimidazole solution;

[0042] S2. Add the 2-methylimidazole solution to the zinc nitrate hexahydrate solution and stir at 300 rpm for 5 min, add 0.1 g of riboflavin (the mass ratio of zinc nitrate hexahydrate, 2-methylimidazole, and riboflavin in this embodiment is 11:24:1), continue stirring at 300 rpm for 10 min, centrifuge the resulting suspension at 10000 rpm for 15 min, and then wash and centrifuge alternately with deionized water and methanol for 3 times to obtain riboflavin microspheres;

[0043] S3. The riboflavin microspheres were placed in a 0.6% cationic chitosan oligosaccharide solution for coating for 30 minutes, washed with deionized water, and dried in a vacuum drying oven at 30°C for 24 hours to obtain chitosan oligosaccharide-coated riboflavin microcapsule powder.

[0044] Preparation Example 3

[0045] S1. Dissolve 1.2 g of zinc nitrate hexahydrate in 21.6 g of deionized water (the mass ratio of deionized water to zinc nitrate hexahydrate in this embodiment is 18:1) to prepare a zinc nitrate hexahydrate solution; dissolve 2.6 g of 2-methylimidazole in 57.2 g of methanol (the mass ratio of methanol to 2-methylimidazole in this embodiment is 22:1) to prepare a 2-methylimidazole solution;

[0046] S2. Add the 2-methylimidazole solution to the zinc nitrate hexahydrate solution and stir at 300 rpm for 5 min, add 0.1 g of riboflavin (the mass ratio of zinc nitrate hexahydrate, 2-methylimidazole, and riboflavin in this embodiment is 12:26:1), continue stirring at 300 rpm for 10 min, centrifuge the resulting suspension at 10000 rpm for 15 min, and then wash and centrifuge alternately with deionized water and methanol for 3 times to obtain riboflavin microspheres;

[0047] S3. The riboflavin microspheres were placed in a 0.6% cationic chitosan oligosaccharide solution for coating for 30 minutes, washed with deionized water, and dried in a vacuum drying oven at 30°C for 24 hours to obtain chitosan oligosaccharide-coated riboflavin microcapsule powder.

[0048] Preparation Example 4

[0049] S1. 1.3 g of zinc nitrate hexahydrate was dissolved in 23.4 g of deionized water (the mass ratio of deionized water to zinc nitrate hexahydrate in this embodiment is 18:1) to prepare a zinc nitrate hexahydrate solution; 2.8 g of 2-methylimidazole was dissolved in 61.6 g of methanol (the mass ratio of methanol to 2-methylimidazole in this embodiment is 22:1) to prepare a 2-methylimidazole solution;

[0050] S2. Add the 2-methylimidazole solution to the zinc nitrate hexahydrate solution and stir at 300 rpm for 5 min, add 0.1 g of riboflavin (the mass ratio of zinc nitrate hexahydrate, 2-methylimidazole, and riboflavin in this embodiment is 13:28:1), continue stirring at 300 rpm for 10 min, centrifuge the resulting suspension at 10000 rpm for 15 min, and then wash and centrifuge alternately with deionized water and methanol for 3 times to obtain riboflavin microspheres;

[0051] S3. The riboflavin microspheres were placed in a 0.6% cationic chitosan oligosaccharide solution for coating for 30 minutes, washed with deionized water, and dried in a vacuum drying oven at 30°C for 24 hours to obtain chitosan oligosaccharide-coated riboflavin microcapsule powder.

[0052] Preparation Example 5

[0053] S1. 0.9 g of zinc nitrate hexahydrate was dissolved in 16.2 g of deionized water (the mass ratio of deionized water to zinc nitrate hexahydrate in this embodiment is 18:1) to prepare a zinc nitrate hexahydrate solution; 2.0 g of 2-methylimidazole was dissolved in 44 g of methanol (the mass ratio of methanol to 2-methylimidazole in this embodiment is 22:1) to prepare a 2-methylimidazole solution;

[0054] S2. Add the 2-methylimidazole solution to the zinc nitrate hexahydrate solution and stir at 300 rpm for 5 min, add 0.1 g of riboflavin (the mass ratio of zinc nitrate hexahydrate, 2-methylimidazole, and riboflavin in this embodiment is 9:20:1), continue stirring at 300 rpm for 10 min, centrifuge the resulting suspension at 10,000 rpm for 15 min, and then wash and centrifuge alternately with deionized water and methanol for 3 times to obtain riboflavin microspheres;

[0055] S3. The riboflavin microspheres were placed in a 0.6% cationic chitosan oligosaccharide solution for coating for 30 minutes, washed with deionized water, and dried in a vacuum drying oven at 30°C for 24 hours to obtain chitosan oligosaccharide-coated riboflavin microcapsule powder.

[0056] Preparation Example 6

[0057] S1. Dissolve 1 g of zinc nitrate hexahydrate in 20 g of deionized water (the mass ratio of deionized water to zinc nitrate hexahydrate in this embodiment is 20:1) to prepare a zinc nitrate hexahydrate solution; dissolve 2.2 g of 2-methylimidazole in 52.8 g of methanol (the mass ratio of methanol to 2-methylimidazole in this embodiment is 24:1) to prepare a 2-methylimidazole solution;

[0058] S2. The 2-methylimidazole solution was added to the zinc nitrate hexahydrate solution and stirred at 400 rpm for 8 min, 0.1 g of riboflavin was added (the mass ratio of zinc nitrate hexahydrate, 2-methylimidazole, and riboflavin in this embodiment was 10:22:1) and the stirring was continued at 400 rpm for 15 min. The resulting suspension was centrifuged at 12500 rpm for 17 min, and then washed and centrifuged alternately with deionized water and methanol for 4 times to obtain riboflavin microspheres;

[0059] S3. The riboflavin microspheres were placed in a 0.6% cationic chitosan oligosaccharide solution for coating for 35 minutes, washed with deionized water, and dried in a vacuum drying oven at 35°C for 27 hours to obtain chitosan oligosaccharide-coated riboflavin microcapsule powder.

[0060] Preparation Example 7

[0061] S1. Dissolve 1 g of zinc nitrate hexahydrate in 22 g of deionized water (the mass ratio of deionized water to zinc nitrate hexahydrate in this embodiment is 22:1) to prepare a zinc nitrate hexahydrate solution; dissolve 2.2 g of 2-methylimidazole in 57.2 g of methanol (the mass ratio of methanol to 2-methylimidazole in this embodiment is 26:1) to prepare a 2-methylimidazole solution;

[0062] S2. Add the 2-methylimidazole solution to the zinc nitrate hexahydrate solution and stir at 500 rpm for 10 min, add 0.1 g of riboflavin (the mass ratio of zinc nitrate hexahydrate, 2-methylimidazole, and riboflavin in this embodiment is 10:22:1), continue stirring at 500 rpm for 20 min, centrifuge the resulting suspension at 15000 rpm for 20 min, and then wash and centrifuge alternately with deionized water and methanol for 5 times to obtain riboflavin microspheres;

[0063] S3. The riboflavin microspheres were placed in a 0.6% cationic chitosan oligosaccharide solution for coating for 40 minutes, washed with deionized water, and dried in a vacuum drying oven at 40°C for 30 hours to obtain chitosan oligosaccharide-coated riboflavin microcapsule powder.

[0064] Preparation Example 8

[0065] The difference between Preparation Example 8 and Preparation Example 1 is that the concentration of the cationic chitosan oligosaccharide solution used in Preparation Example 8 is 0.8%.

[0066] Preparation Example 9

[0067] The difference between Preparation Example 9 and Preparation Example 1 is that the concentration of the cationic chitosan oligosaccharide solution used in Preparation Example 9 is 0.9%.

[0068] Preparation Example 10

[0069] The difference between Preparation Example 10 and Preparation Example 1 is that the concentration of the cationic chitosan oligosaccharide solution used in Preparation Example 10 is 0.4%.

[0070] Preparation Example 11

[0071] The difference between Preparation Example 11 and Preparation Example 1 is that the concentration of the cationic chitosan oligosaccharide solution used in Preparation Example 11 is 1.1%.

[0072] Example

[0073] Example 1

[0074] A synergist capable of improving the wintering rate of alfalfa, comprising the following raw materials: 0.5 kg of brassinolide, 1 kg of chitosan oligosaccharide coated riboflavin microcapsule powder, 6 kg of brown sugar, and 800 kg of water;

[0075] The chitosan oligosaccharide-coated riboflavin microcapsule powder used in this example comes from Preparation Example 1.

[0076] Example 2

[0077] A synergist capable of improving the wintering rate of alfalfa, comprising the following raw materials: 0.8 kg of brassinolide, 1.5 kg of chitosan oligosaccharide-coated riboflavin microcapsule powder, 8 kg of brown sugar, and 900 kg of water;

[0078] The chitosan oligosaccharide-coated riboflavin microcapsule powder used in this example comes from Preparation Example 1.

[0079] Example 3

[0080] A synergist capable of improving the wintering rate of alfalfa, comprising the following raw materials: 1kg of brassinolide, 2kg of chitosan oligosaccharide-coated riboflavin microcapsule powder, 10kg of brown sugar, and 1000kg of water;

[0081] The chitosan oligosaccharide-coated riboflavin microcapsule powder used in this example comes from Preparation Example 1.

[0082] Example 4

[0083] A synergist capable of improving the wintering rate of alfalfa, comprising the following raw materials: 1.2 kg of brassinolide, 2.5 kg of chitosan oligosaccharide coated riboflavin microcapsule powder, 12 kg of brown sugar, and 1100 kg of water;

[0084] The chitosan oligosaccharide-coated riboflavin microcapsule powder used in this example comes from Preparation Example 1.

[0085] Example 5

[0086] A synergist capable of improving the wintering rate of alfalfa, comprising the following raw materials: 1.5 kg of brassinolide, 3 kg of chitosan oligosaccharide-coated riboflavin microcapsule powder, 14 kg of brown sugar, and 1200 kg of water;

[0087] The chitosan oligosaccharide-coated riboflavin microcapsule powder used in this example comes from Preparation Example 1.

[0088] Example 6

[0089] The difference between Example 6 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 6 comes from Preparation Example 2.

[0090] Example 7

[0091] The difference between Example 7 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 7 comes from Preparation Example 3.

[0092] Example 8

[0093] The difference between Example 8 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 8 comes from Preparation Example 4.

[0094] Example 9

[0095] The difference between Example 9 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 9 comes from Preparation Example 5.

[0096] Example 10

[0097] The difference between Example 10 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 10 comes from Preparation Example 6.

[0098] Embodiment 11

[0099] The difference between Example 11 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 11 comes from Preparation Example 7.

[0100] Example 12

[0101] The difference between Example 12 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 12 comes from Preparation Example 8.

[0102] Embodiment 13

[0103] The difference between Example 13 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 13 comes from Preparation Example 9.

[0104] Embodiment 14

[0105] The difference between Example 14 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 14 comes from Preparation Example 10.

[0106] Embodiment 15

[0107] The difference between Example 15 and Example 1 is that the chitosan oligosaccharide-coated riboflavin microcapsule powder used in Example 15 comes from Preparation Example 11.

[0108] Comparative Example

[0109] Comparative Example 1

[0110] The difference between Comparative Example 1 and Example 1 is that brassinolide is not included in Comparative Example 1. The synergist capable of improving the wintering rate of alfalfa is composed of the following raw materials: 1 kg of chitosan oligosaccharide-coated riboflavin microcapsule powder, 8 kg of brown sugar, and 800 kg of water;

[0111] The chitosan oligosaccharide-coated riboflavin microcapsule powder used in this comparative example comes from Preparation Example 1.

[0112] Comparative Example 2

[0113] The difference between Comparative Example 2 and Example 1 is that brown sugar is not included in Comparative Example 2. The synergist capable of improving the wintering rate of alfalfa is composed of the following raw materials: 0.5 kg of brassinolide, 1 kg of chitosan oligosaccharide-coated riboflavin microcapsule powder, and 800 kg of water;

[0114] The chitosan oligosaccharide-coated riboflavin microcapsule powder used in this example comes from Preparation Example 1.

[0115] Comparative Example 3

[0116] The difference between Comparative Example 3 and Example 1 is that riboflavin is not included in Comparative Example 3, and the synergist capable of improving the wintering rate of alfalfa is composed of the following raw materials: 0.5 kg of brassinolide, 6 kg of brown sugar, and 800 kg of water.

[0117] Comparative Example 4

[0118] The difference between Comparative Example 4 and Example 1 is that the riboflavin used in Comparative Example 4 is ordinary riboflavin that is not made into microcapsules. The synergist that can improve the wintering rate of alfalfa is composed of the following raw materials: 0.5 kg of brassinolide, 1 kg of riboflavin, 6 kg of brown sugar, and 800 kg of water.

[0119] Application Examples

[0120] Application Example 1

[0121] Step 1: Prepare alfalfa fields for crop rotation, cut the above-ground parts, take out the root necks and branch buds as a whole, cut the root necks and branch buds of the whole plant into alfalfa sprout particles with a diameter of 0.5 cm by mechanical equipment, and spray the alfalfa sprout particles with the synergist of the formula in Example 1 for 2 hours;

[0122] Step 2: Sow the sprayed alfalfa sprout particles at a sowing depth of 2 cm. The row spacing for forage fields is 15 cm, and the row spacing for seed fields is 30 cm. The sowing amount is 100 kg / mu.

[0123] Application Example 2

[0124] Step 1: Prepare alfalfa fields for crop rotation, cut the above-ground parts, take out the root necks and branch buds as a whole, cut the root necks and branch buds of the whole plant into alfalfa sprout particles with a diameter of 0.8 cm by mechanical equipment, and spray the alfalfa sprout particles with the synergist of the formula in Example 1 for 2 hours;

[0125] Step 2: Sow the sprayed alfalfa sprout particles at a sowing depth of 2.5 cm. Select 18 cm row spacing for forage fields and 35 cm row spacing for seed fields. Sowing rate is 150 kg / mu.

[0126] Application Example 3

[0127] Step 1: Prepare alfalfa fields for crop rotation, cut the above-ground parts, take out the root necks and branch buds as a whole, cut the root necks and branch buds of the whole plant into alfalfa sprout particles with a diameter of 1 cm by mechanical equipment, and spray the alfalfa sprout particles with the synergist of the formula in Example 1 for 2 hours;

[0128] Step 2: Sow the sprayed alfalfa sprout particles at a sowing depth of 3 cm, select a row spacing of 20 cm for forage fields and a row spacing of 40 cm for seed fields, and a sowing rate of 200 kg / mu.

[0129] Application Example 4

[0130] The difference between Application Example 4 and Application Example 1 is that the spraying treatment time in Application Example 4 is 3 hours.

[0131] Application Example 5

[0132] The difference between Application Example 5 and Application Example 1 is that the spraying treatment time in Application Example 5 is 4 hours.

[0133] Application Example 6

[0134] The difference between Application Example 6 and Application Example 1 is that the spraying treatment time in Application Example 6 is 1 hour.

[0135] Application Example 7

[0136] The difference between Application Example 7 and Application Example 1 is that the spraying treatment time in Application Example 7 is 5 hours.

[0137] Application Example 8-21

[0138] The difference between Application Example 8-21 and Application Example 1 is that the synergist formula used in Application Example 8-21 is from Example 2-15.

[0139] Comparative Application Examples 1-4

[0140] The difference between Comparative Application Examples 1-4 and Application Example 1 is that the synergist formula used in Comparative Application Examples 1-4 comes from Comparative Examples 1-4.

[0141] Performance testing

[0142] 1. The final wintering rate of alfalfa corresponding to the planting methods of Example 1-21 and Comparative Application Example 1-4 was tested and calculated, and the results are shown in Table 1.

[0143] The specific test results are as follows:

[0144] Table 1 Performance test results

[0145]

[0146]

[0147] It can be seen from the test results in Table 1 that the synergist and planting method for improving the wintering rate of alfalfa provided in the present application have a strong yield-increasing effect, effectively improve the cold resistance and stress resistance of alfalfa, and enhance the wintering rate. The wintering rate reaches more than 96%, effectively realizing the utilization and large-scale utilization of the alfalfa sprout bank.

[0148] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A synergist capable of improving the wintering rate of alfalfa, characterized in that: The raw materials include brassinolide, chitosan oligosaccharide coated riboflavin microcapsule powder, brown sugar and water; the mass ratio of the brassinolide, chitosan oligosaccharide coated riboflavin microcapsule powder, brown sugar and water is 0.5-1.5:1-3:6-14:800-1200.

2. A synergist capable of improving the wintering rate of alfalfa according to claim 1, characterized in that: The mass ratio of the brassinolide, chitosan oligosaccharide coated riboflavin microcapsule powder, brown sugar and water is 0.8-1.2:1.5-2.5:8-12:900-1100.

3. A synergist capable of improving the wintering rate of alfalfa according to claim 2, characterized in that: The mass ratio of the brassinolide, chitosan oligosaccharide-coated riboflavin microcapsule powder, brown sugar and water is 1:2:10:1000.

4. A synergist capable of improving the wintering rate of alfalfa according to claim 2 or 3, characterized in that: The preparation method of the chitosan oligosaccharide coated riboflavin microcapsule powder comprises the following steps: S1. Dissolving zinc nitrate hexahydrate in deionized water to prepare a zinc nitrate hexahydrate solution; dissolving 2-methylimidazole in methanol to prepare a 2-methylimidazole solution; S2. Add 2-methylimidazole solution to zinc nitrate hexahydrate solution and stir for 5-10 minutes, add riboflavin and continue stirring for 10-20 minutes, centrifuge the resulting suspension at a speed of 10000-15000 rpm for 15-20 minutes, and then wash and centrifuge several times with deionized water and methanol alternately to obtain riboflavin microspheres; S3. Place the riboflavin microspheres in the cationic chitosan oligosaccharide solution for coating for 30-40 minutes, wash with deionized water, and dry in a vacuum drying oven at 30-40°C for 24-30 hours to obtain chitosan oligosaccharide coated riboflavin microcapsule powder.

5. A synergist capable of improving the wintering rate of alfalfa according to claim 4, characterized in that: The mass ratio of zinc nitrate hexahydrate, 2-methylimidazole and riboflavin is 10-12:22-26:

1.

6. A synergist capable of improving the wintering rate of alfalfa according to claim 4, characterized in that: The mass ratio of deionized water to zinc nitrate hexahydrate in S1 is 18-22:

1.

7. The synergist for improving the wintering rate of alfalfa according to claim 4, characterized in that: The mass ratio of methanol to 2-methylimidazole in S2 is 22-26:

1.

8. The synergist for improving the wintering rate of alfalfa according to claim 4, characterized in that: The concentration of the cationic chitosan oligosaccharide solution in S3 is 0.6-0.9%.

9. A planting method for increasing the wintering rate of alfalfa, characterized in that: The following steps are involved: Step 1: Prepare the alfalfa field for crop rotation, mow the above-ground part, take out the root neck and branch buds as a whole, cut the root neck and branch buds of the whole plant into alfalfa sprout particles with a diameter of 0.5-1cm through mechanical equipment, and spray the alfalfa sprout particles with a synergist; Step 2: Sow the sprayed alfalfa sprout particles at a sowing depth of 2-3 cm, select a row spacing of 15-20 cm for forage fields, select a row spacing of 30-40 cm for seed fields, and a sowing amount of 100-200 kg / mu.

10. A planting method for increasing the wintering rate of alfalfa according to claim 9, characterized in that: The spraying treatment time in step 1 is 2-4 hours.

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