Polypeptide synthesis equipment

Abstract

The embodiment of the invention provides polypeptide synthesis equipment. The polypeptide synthesis equipment comprises: a reaction container device, the reaction container device is provided with a reaction container, and the interior of the reaction container is used for reaction; the liquid adding device is used for adding liquid into the reaction container; the reaction container is arranged on the shaking-up device, and the shaking-up device is used for driving the reaction container to swing; and the bubbling device is connected to the reaction container and is used for blowing gas into the reaction container. The polypeptide synthesis equipment provided by the embodiment of the invention can realize automatic polypeptide synthesis. Therefore, the polypeptide synthesis equipment can save labor, reduce the labor cost, improve the production efficiency and meet the large-flux production requirement. Moreover, manual intervention is reduced in the production process, so that the error rate is relatively low, and the effects of reducing personnel pollution and realizing clean production are achieved, so that relatively high safety of polypeptide synthesis can be ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of polypeptide synthesis, and in particular to a polypeptide synthesis device. Background Art

[0002] As a key area of ​​innovative research and development in biomedicine, peptide synthesis technology has broad development prospects. Peptide drugs have become a hot spot in global new drug research and development due to their high biological activity, high specificity, low side effects, and low accumulation in the body. Currently, more than 80 peptide drugs have been approved for marketing worldwide, with a market size of US$62.8 billion in 2020 and expected to grow to US$96 billion in 2025, showing a strong growth momentum.

[0003] Conventional peptide synthesis equipment includes a reaction vessel. A resin is placed in the reaction vessel. The resin can be used as a solid phase carrier, which is usually pre-activated and has a protecting group such as Fmoc (fluorenylmethoxycarbonyl). A 20% piperidine solution can be added to the reaction cup, and the piperidine solution can deprotect the resin and remove the Fmoc protecting group at the N-terminus. Then, a gas such as nitrogen can be added to the reaction vessel so that bubbling (condensation reaction) can be performed. Then, subsequent steps can be performed to eventually connect all amino acids to the resin in a predetermined order to form a complete polypeptide chain.

[0004] However, there are many problems with traditional peptide synthesis equipment. First, traditional peptide synthesis equipment requires too much manual participation, resulting in high labor costs. In addition, the labor efficiency is low, and cross-cooperation between personnel is required, resulting in a high probability of error, resulting in a low throughput of synthesized peptides and unable to meet customer needs. In addition, personnel are prone to product contamination, causing customer complaints, and in severe cases, causing loss of patient life. Summary of the invention

[0005] In order to at least partially solve the problems existing in the prior art, an embodiment of the present invention provides a polypeptide synthesis device. The polypeptide synthesis device includes: a reaction container device, the reaction container device has a reaction container, and the reaction container is used for reaction; a liquid adding device, the liquid adding device is used to add liquid into the reaction container; a shaking device, the reaction container is arranged on the shaking device, and the shaking device is used to drive the reaction container to shake; and a bubbling device, the bubbling device is connected to the reaction container, and is used to bubbling gas into the reaction container.

[0006] Exemplarily, the shaking device includes a shaking drive assembly, a turntable, a shaking plate and an eccentric member, wherein the driving end of the shaking drive assembly is connected to the turntable to drive the turntable to rotate around a rotation axis, the reaction container is arranged on the shaking plate, the eccentric member is connected between the turntable and the shaking plate, the eccentric member is not coaxial with the rotation axis, and the eccentric member drives the shaking plate and the reaction container to swing along a first straight line direction perpendicular to the rotation axis under the drive of the shaking drive assembly.

[0007] Exemplarily, the shaking device also includes a connecting plate, which is provided with a long groove extending along a second straight line direction perpendicular to the rotation axis and the first straight line direction. The eccentric piece is inserted into the long groove and can swing in the long groove under the drive of the shaking drive assembly. The connecting plate is connected to the shaking plate.

[0008] Exemplarily, the bubbling device includes a gas source triplet, a stop valve and a speed regulating valve. Along the flow direction of the gas bubbled into the bubbling device, the gas source triplet, the stop valve, the speed regulating valve and the reaction container are connected in sequence.

[0009] Exemplarily, the reaction vessel device includes multiple groups of reaction vessel assemblies, each group of reaction vessel assemblies includes multiple reaction vessels, the bubbling device is arranged in a one-to-one correspondence with the multiple groups of reaction vessel assemblies, the bubbling device also includes a diverter, the diverter is provided with an air inlet and a plurality of air outlets, the plurality of air outlets are arranged in a one-to-one correspondence with all the reaction containers of the corresponding reaction vessel assemblies, the air inlet passes through the diverter, one end of the stop valve is connected to the gas source triplet, and the other end is connected to the two ends of the air inlet, one end of the plurality of air outlets is respectively connected to the middle part between the two ends of the air inlet, and the speed regulating valve is connected one-to-one between the other end of the plurality of air outlets and the reaction vessel.

[0010] Exemplarily, the peptide synthesis device further comprises a heating device for heating the reaction container, the heating device is arranged on the shaking device, and the shaking device is used to drive the reaction container and the heating device to shake synchronously.

[0011] Exemplarily, the heating device includes a support plate and a heating element, wherein the support plate is disposed on the shaking device, the heating element is disposed on the support plate, a placement hole and a heating circulation channel surrounding the placement hole are disposed on the heating element, and the reaction container can be detachably inserted into the placement hole.

[0012] Exemplarily, the reaction vessel device includes multiple groups of reaction vessel assemblies, each group of reaction vessel assemblies includes multiple reaction vessels, the heating devices are arranged in one-to-one correspondence with the multiple groups of bubbling devices, a plurality of placement holes are arranged at intervals on the heating element, the plurality of placement holes are used for one-to-one insertion of all the reaction containers of the corresponding reaction vessel assemblies, and the heating circulation channel surrounds the plurality of placement holes.

[0013] Exemplarily, the top of the reaction container is provided with a top opening and the bottom is provided with a drainage port. The polypeptide synthesis equipment also includes a drainage device, which includes a positive pressure drainage component and a negative pressure drainage component. The positive pressure drainage component is used to inject gas into the reaction container from the top opening to apply pressure to the reaction container, and the negative pressure drainage component is connected to the drainage port to extract the liquid in the reaction container through the drainage port.

[0014] Exemplarily, the drainage device further includes a drainage pipeline and a tube clamping assembly, the drainage pipeline is connected between the negative pressure drainage assembly and the drainage port, and the tube clamping assembly is used to clamp the drainage pipeline to cut off the drainage pipeline.

[0015] Exemplarily, the reaction container device includes multiple groups of reaction container assemblies, each group of reaction container assemblies includes multiple reaction containers, the drainage pipeline includes multiple branch drainage pipelines, the multiple branch drainage pipelines are arranged in a one-to-one correspondence with all the reaction containers of the corresponding reaction container assemblies, the branch drainage pipeline is connected between the negative pressure drainage assembly and its corresponding drainage port, and the tube clamping assembly is used to clamp the multiple branch drainage pipelines to cut off the multiple branch drainage pipelines.

[0016] Exemplarily, the reaction vessel device includes multiple groups of reaction vessel assemblies, each group of reaction vessel assemblies includes multiple reaction containers, the positive pressure drainage assembly is arranged in a one-to-one correspondence with all the reaction containers of the corresponding reaction vessel assembly, the positive pressure drainage assembly includes a three-way joint and a connecting pipe, the first end of the three-way joint is one-to-one correspondingly toward each top opening in the corresponding reaction vessel assembly, along the arrangement direction of the three-way joints, the second end of the first three-way joint is used to connect to the gas source, the third end of the last three-way joint is sealed with a plug, and the connecting pipe is connected between the second end and the third end of two adjacent three-way joints.

[0017] Exemplarily, the liquid adding device includes multiple liquid injection parts, liquid outlet parts and multiple switching valves corresponding to the multiple liquid injection parts one by one. The switching valve is arranged between the corresponding liquid injection parts and the multiple liquid outlet parts. The switching valve has a liquid suction state that connects the corresponding liquid injection part with the liquid storage container and a liquid injection state that connects the corresponding liquid injection part with the liquid outlet part. The liquid injection part is used to suck the liquid in the liquid storage container into the liquid injection part when the corresponding switching valve is in the liquid suction state, and is used to inject the liquid in the liquid injection part into the reaction container below the liquid outlet part through the liquid outlet part when the corresponding switching valve is in the liquid injection state.

[0018] Exemplarily, the reaction container includes a reaction cup, a supporting member and a feed tube. The reaction cup is used for conducting a reaction. A bottom opening is provided at the bottom of the reaction cup. The reaction cup is supported on the supporting member, and the supporting member is provided on a shaking device. The feed tube extends downward from the supporting member. A liquid inlet and an air inlet connected to the bottom opening are provided on the side of the feed tube. A liquid adding device is connected to the liquid inlet for adding liquid into the reaction cup. A bubbling device is connected to the air inlet for bubbling gas into the reaction cup.

[0019] The peptide synthesis device provided in the embodiment of the present invention can realize automatic synthesis of peptides through the coordination of the reaction container device, the liquid adding device, the shaking device and the bubbling device. In this way, the peptide synthesis device can save labor, reduce labor costs, improve production efficiency, and meet the production needs of large throughput. In addition, since human intervention is reduced in the production process, the error rate is low, and it has the effect of reducing human pollution and clean production, thereby ensuring that the safety of peptide synthesis is high.

[0020] A series of simplified concepts are introduced in the summary of the invention, which will be further described in detail in the detailed description. The summary of the invention does not mean to attempt to define the key features and essential technical features of the claimed technical solution, nor does it mean to attempt to determine the scope of protection of the claimed technical solution.

[0021] The advantages and features of the present invention are described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The following drawings of the present invention are used as part of the present invention for understanding the present invention. The drawings show the embodiments of the present invention and their descriptions, and are used to explain the principles of the present invention. In the drawings,

[0023] Figure 1 A perspective view of a polypeptide synthesis device according to an exemplary embodiment of the present invention;

[0024] Figure 2 for Figure 1 A perspective view of a partial structure of a peptide synthesis device shown in FIG.

[0025] Figure 3 for Figure 2 A perspective view of a partial structure of a peptide synthesis device shown in another angle;

[0026] Figure 4 for Figure 3 A partial enlarged view of the internal structure of the peptide synthesis device shown in FIG.

[0027] Figure 5 for Figure 2A top view of the lower frame of the peptide synthesis device and its internal structure shown in;

[0028] Figure 6 for Figure 2 A stereogram of a partial structure of a peptide synthesis device shown in FIG.

[0029] Figure 7 for Figure 6 A side view of a partial structure of a peptide synthesis device shown in FIG.

[0030] Figure 8 for Figure 7 A perspective view of a partial structure of the liquid discharge device shown in FIG.

[0031] Fig. 9 for Figure 8 A front view of a partial structure of the discharge device shown in FIG.

[0032] Fig.10 for Figure 6 A perspective view of the shaking device shown in FIG.

[0033] Fig.11 for Fig.10 An exploded view of the shaking device shown in FIG.

[0034] Fig.12 for Figure 6 A stereogram of a partial structure of a peptide synthesis device shown in FIG.

[0035] Fig.13 for Fig.12 A perspective view of the heating element shown in FIG.

[0036] Fig.14 for Fig.12 A cross-sectional view of the heating element shown in FIG.

[0037] Fig.15 for Fig.12 A bottom view of the heating element shown in FIG.

[0038] Fig.16 for Fig.12 A front view of the reaction vessel shown in;

[0039] Fig.17 for Fig.16 A cross-sectional view of a reaction vessel shown in FIG.

[0040] Fig.18 for Fig.17 A cross-sectional view of a reaction cup shown in FIG.

[0041] Fig.19 for Figure 2 A perspective view of a partial structure of a liquid adding device shown in FIG.

[0042] Fig. 20 for Fig.19 A perspective view of a partial structure of a liquid adding device shown in FIG.

[0043] Fig.21 for Fig. 20 A front view of the first liquid outlet needle shown in;

[0044] Fig. 22 for Fig.21 A cross-sectional view of the first liquid outlet needle shown in FIG.

[0045] Fig.23 for Fig. 20 A cross-sectional view of the second liquid outlet needle shown in FIG.

[0046] Fig.24 for Figure 5 A stereoscopic view of the negative pressure tank and related structures is shown in FIG.

[0047] The above drawings include the following reference numerals:

[0048] 1000, reaction vessel device; 1100, reaction vessel; 1110, reaction cup; 1111, bottom opening; 1112, top opening; 1113, large size section; 1114, small size section; 1115, transition section; 1120, supporting member; 1121, cup holder; 1122, sealing ring; 1130, feeding pipe; 1131, liquid inlet; 1132, air inlet; 1133, feeding channel; 1134, liquid outlet; 1140, filter screen; 1200, reaction vessel assembly; 2000, liquid adding device; 2100, liquid injection member; 2110, motor; 2120, syringe; 2200, liquid outlet member; 2210, first liquid outlet needle; 2211, first liquid outlet hole; 2220, second liquid outlet needle; 2221, needle tube; 2222, umbrella cover; 2223, second liquid outlet; 2230, liquid receiving tank drive; 2240, liquid receiving tank; 2250, second liquid outlet needle drive; 2300, switching valve; 2400, injection pump; 2500, multi-valve; 2600, mounting plate; 2700, floating mechanism; 2710, floating body; 2720, floating member; 2721, sensor; 2730, floating detector; 2731, transmitter; 2732, receiver; 2810, first horizontal drive; 2820, second horizontal drive; 2830, vertical drive; 2900, liquid discharge container; 3000, shaking device; 3100, shaking drive assembly; 3110, motor; 312 0, reducer; 3200, turntable; 3300, shake plate; 3400, eccentric piece; 3410, mounting shaft; 3420, roller; 3500, connecting plate; 3510, long groove; 3600, guide rail assembly; 3601, first guide rail assembly; 3602, second guide rail assembly; 3610, connecting block; 3620, guide rail; 3630, slider; 3700, mounting plate; 3710, through hole; 4000, bubbling device; 4100, speed regulating valve; 4200, diverter; 4210, air inlet; 4220, air outlet; 4310, three-way joint; 4320, diverter pipeline; 4330, branch pipeline; 5000, heating device; 5100, support plate; 5200, heating element; 52 10. Placement hole; 5220. Heating circulation channel; 5221. Inlet; 5222. Outlet; 5223. First heating hole; 5224. Second heating hole; 5225. Third heating hole; 5226. Fourth heating hole; 5230. Observation hole; 5240. Sensor hole; 6000. Discharge device; 6100. Positive pressure discharge assembly; 6110. Three-way joint; 6120. Air pressure detector; 6200. Negative pressure discharge assembly; 6210. Negative pressure tank; 6211. Negative pressure port; 6212. Liquid inlet; 6213. Positive pressure port; 6214. Spare port; 6220. Vacuum pump; 6230. Liquid stop valve; 6240. Liquid level pipeline; 6241. Transparent window; 6250. Switch valve;6300, drainage pipeline; 6310, shunt drainage pipeline; 6320, confluence piece; 6400, clamping tube assembly; 6410, clamping tube body; 6411, limiting hole; 6420, clamping tube driving member; 6430, pushing member; 6431, pushing member surface; 7000, opening and closing cover device; 7100, opening and closing cover driving member; 7200, cover plate; 7210, elastic layer; 7300, lateral movement driving member; 9100, rack; 9110, cabinet; 9111, installation platform; 9120, cover; 9121, transparent window; 9200, thermostat; 9310, electric control cabinet; 9320, bridge; 9410, first fan; 9420, second fan; 9430, negative pressure gauge; 9440, axial flow fan; 9450, display screen. ; DETAILED DESCRIPTION

[0049] In the following description, a large amount of details are provided so that the present invention can be thoroughly understood. However, it will be appreciated by those skilled in the art that the following description only exemplarily illustrates a preferred embodiment of the present invention, and the present invention can be implemented without one or more such details. In addition, in order to avoid confusion with the present invention, some technical features well known in the art are not described in detail.

[0050] The embodiment of the present invention provides a peptide synthesis device. The peptide synthesis device can be used to synthesize peptides. The peptide synthesis device of the embodiment of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0051] like Figure 1-3 As shown in Figure 6, the peptide synthesis device may include a reaction vessel device 1000, a liquid adding device 2000, a shaking device 3000 and a bubbling device 4000. The reaction vessel device 1000, the liquid adding device 2000, the shaking device 3000 and the bubbling device 4000 may be arranged at any suitable position. In some embodiments, the peptide synthesis device may further include a rack 9100. The rack 9100 may include a cabinet 9110 and a cover 9120 located above the cabinet 9110. The cabinet 9110 may be seated on the ground or on a work platform. The top wall of the cabinet 9110 may be a mounting platform 9111. The reaction vessel device 1000, the liquid adding device 2000, the shaking device 3000 and the bubbling device 4000 may all be located on the mounting platform 9111. The cover 9120 can be installed on the mounting platform 9111, so as to cover the reaction container device 1000, the liquid adding device 2000, the shaking device 3000 and the bubbling device 4000. It should be noted that the space inside the cabinet 9110 and the cover 9120 can be connected, and the gas can flow between the two.

[0052] Since the liquid used by the peptide synthesis equipment to synthesize peptides is corrosive, the cabinet 9110 and the cover 9120 can be made of corrosion-resistant materials. Exemplarily, the cabinet 9110 and the cover 9120 can be made of stainless steel (e.g., stainless steel 304). One or more transparent windows 9121 can be provided on the side wall of the cover 9120, so that relevant personnel can observe the reaction inside the cover 9120 through the transparent windows 9121 from outside the cover 9120. The transparent window 9121 can be made of transparent materials such as glass or acrylic. Since the liquid used by the peptide synthesis equipment to synthesize peptides is also flammable and explosive, the transparent window 9121 can be made of tempered glass, which can reduce the harm to relevant personnel in dangerous situations such as explosions. In addition, the following related pressure gauges, motors and / or electric control cabinets and other related components can all use components that meet explosion-proof requirements. Since the liquid used by the peptide synthesis device to synthesize peptides is also volatile, the top wall of the cover 9120 can be provided with a first fan 9410 and a second fan 9420. After the liquid evaporates, gas stratification will be formed, and the lighter gas will float in the cover 9120, and the heavier gas will settle in the cabinet 9110. The first fan 9410 can be connected to the cabinet 9110 to discharge the gas in the cabinet 9110. The second fan 9420 can be connected to the cover 9120 to discharge the gas in the cover 9120. The first fan 9410 and the second fan 9420 can discharge the gas into the waste pipe, thereby playing the role of negative pressure exhaust. Both the first fan 9410 and the second fan 9420 can adopt explosion-proof fans. The side wall of the cabinet 9110 can be provided with one or more axial flow fans 9440. The axial flow fan 9440 can draw air outside the peptide synthesis device into the rack 9100, thereby playing the role of positive pressure air intake, and then cooperate with the first fan 9410 and the second fan 9420 to perform negative pressure exhaust. A negative pressure gauge 9430 can also be provided on the side wall of the cover body 9120. The negative pressure gauge 9430 can be used to display the negative pressure value in the rack 9100. If the exhaust is blocked or the first fan 9410 and the second fan 9420 fail during operation, the reading of the negative pressure gauge 9430 will be abnormal, and then the peptide synthesis device can be controlled to sound an alarm. A display screen 9450 located near the negative pressure gauge 9430 can also be provided on the side wall of the cover body 9120. The display screen 9450 can be used to display the reaction progress, temperature, humidity and / or other relevant data in the peptide synthesis device.

[0053] For example, Figure 1As shown, the peptide synthesis equipment may also include an electric control cabinet 9310 and a bridge 9320. The electric control cabinet 9310 may be located on one side of the rack 9100. The electric control cabinet 9310 may be provided with electrical components such as a PLC and a power supply. The bridge 9320 may be connected between the top of the electric control cabinet 9310 and the top of the cover 9120. The cables may be connected between the relevant components in the rack 9100 and the relevant components in the electric control cabinet 9310 through the bridge 9320, so that electrical connection may be achieved. With such a configuration, even if there is liquid leakage in the rack 9100, the liquid will not enter the electric control cabinet 9310, and the electric control cabinet 9310 has a higher safety. The electric control cabinet 9310 may be an explosion-proof electric control cabinet. Exemplarily, the housing of the electric control cabinet 9310 may be made of 3 mm thick carbon steel. The top wall of the electric control cabinet 9310 may be provided with an explosion-proof cable gland. The cables in the electric control cabinet 9310 can extend through the explosion-proof cable gland, so that they can enter the rack 9100 through the bridge 9320. The explosion-proof cable gland can meet the explosion-proof requirements. The electric control cabinet 9310 can also be provided with an explosion-proof air conditioner. The explosion-proof air conditioner can be used to control and adjust the temperature and humidity in the electric control cabinet 9310, ensure that the components in the electric control cabinet 9310 operate under suitable environmental conditions, and avoid the normal operation and service life of the polypeptide synthesis equipment due to adverse environmental factors such as high temperature and humidity. Exemplarily, when the temperature in the electric control cabinet 9310 rises, the refrigeration system of the explosion-proof air conditioner can start working, so that heat can be absorbed and discharged to the outside of the scene (such as a factory building) where the polypeptide synthesis equipment is located through the refrigerant circulation, and the air in the electric control cabinet 9310 can be circulated to ensure uniform temperature distribution, and it has filtering and dehumidification functions to keep the environment in the electric control cabinet 9310 clean and dry.

[0054] The reaction vessel device 1000 may have one or more reaction vessels 1100. The reaction vessel 1100 may be used for reaction, so that a polypeptide may be generated. Exemplarily, the reaction vessel device 1000 may include multiple groups of reaction vessel assemblies 1200, such as two groups, three groups or more. In the embodiment shown in the figure, the reaction vessel device 1000 may include six groups of reaction vessel assemblies 1200. Each group of reaction vessel assemblies 1200 may include multiple reaction vessels 1100, such as two, three or more. In the embodiment shown in the figure, each group of reaction vessel assemblies 1200 may include ten reaction vessels 1100. The ten reaction vessels 1100 may be arranged in a straight line. In this arrangement, reactions may be carried out in sixty reaction vessels 1100, respectively, so that the rate of synthesizing polypeptides in the polypeptide synthesis device is high, thereby improving the throughput of synthesizing polypeptides. At least two of the multiple groups of reaction vessel assemblies 1200 may be staggered in the vertical direction. In the embodiment shown in the figure, the six groups of reaction vessel assemblies 1200 may have three pairs, each pair may include two groups of reaction vessel assemblies 1200 staggered in the vertical direction, and the three pairs may be arranged at intervals in the straight direction. The reaction vessel assemblies 1200 staggered in the vertical direction may facilitate observation by relevant personnel and pipeline routing.

[0055] The liquid adding device 2000 can be used to add liquid into the reaction vessel 1100. The liquid includes but is not limited to DMF (dimethylformamide) solution and / or piperidine solution. Specifically, the liquid adding device 2000 can be used to be connected to a liquid storage container for storing liquid. The liquid adding device 2000 can suck out the liquid in the liquid storage container, so that it can be filled into the reaction vessel 1100.

[0056] The reaction container 1100 can be arranged on the shaking device 3000 by any suitable means such as welding, clamping or connecting with a connector. The shaking device 3000 can be used to drive the reaction container 1100 to shake. The trajectory of the shaking includes but is not limited to a straight line or an arc similar to a pendulum.

[0057] The bubbling device 4000 can be connected to the reaction vessel 1100. The bubbling device 4000 can be used to bubbling gas into the reaction vessel 1100. The gas includes but is not limited to nitrogen, and the following description is taken as an example of nitrogen. Specifically, the bubbling device 4000 can be used to be connected to the first gas source. The liquid adding device 2000 can suck out the nitrogen provided by the first gas source, so that it can be bubbling into the reaction vessel 1100.

[0058] The following schematically describes the workflow of the peptide synthesis device. First, the relevant personnel can add the resin into the reaction vessel 1100 manually or by a handling device such as a manipulator. Then, the liquid adding device 2000 can add the piperidine solution into the reaction vessel 1100. Then, the shaking device 3000 and the bubbling device 4000 can work. Specifically, the shaking device 3000 can drive the reaction vessel 1100 to shake, so that the piperidine solution and the resin can be fully contacted, thereby accelerating the reaction speed. In addition, the bubbling device 4000 can blow nitrogen into the reaction vessel 1100. Nitrogen can stir the piperidine solution and the resin, so that the piperidine solution and the resin can be fully contacted, thereby accelerating the reaction speed. After a period of reaction, the liquid in the reaction vessel 1100 can be discharged to leave the material that needs further reaction. Then, the peptide synthesis device can perform a flushing process. Specifically, the liquid adding device 2000 can add DMF solution into the reaction vessel 1100. Then, the shaking device 3000 and the bubbling device 4000 can be shaken and aerated respectively to speed up the flushing speed. Then, the liquid in the reaction vessel 1100 can be discharged to leave the material that needs further reaction. According to actual needs, the flushing process here can be repeated any number of times, for example, five times. When the flushing process is completed, the relevant personnel can add the amino acid and the coupling agent to the reaction vessel 1100 in a certain proportion by manual or manipulators or other handling devices to react again. Then, the peptide synthesis equipment can perform the above flushing process again. According to actual needs, the flushing process here can be repeated any number of times, for example, four times. After the reaction is completed, the expected polypeptide can be left in the reaction vessel 1100. Then, the relevant personnel can take out the polypeptide in the reaction vessel 1100 by manual or manipulators or other handling devices to complete the unloading. The peptide synthesis equipment can repeat the above workflow to synthesize more polypeptides.

[0059] In summary, the polypeptide synthesis device provided in the embodiment of the present invention can realize automatic polypeptide synthesis through the coordination of the reaction container device 1000, the liquid adding device 2000, the shaking device 3000 and the bubbling device 4000. In this way, the polypeptide synthesis device can save labor, reduce labor costs, improve production efficiency, and meet the production needs of large throughput. In addition, since human intervention is reduced in the production process, the error rate is low, and it has the effect of reducing personnel pollution and clean production, thereby ensuring that the safety of polypeptide synthesis is high.

[0060] For example, Figure 6 , 12As shown in Figures 16-18, the reaction vessel 1100 may include a reaction cup 1110, a support member 1120 and a feed tube 1130. The reaction cup 1110 is used to perform the above reaction. Exemplarily, the reaction cup 1110 may be made of glass or other transparent materials. With such a configuration, relevant personnel can conveniently observe the reaction situation inside the reaction cup 1110 from the outside of the reaction cup 1110. The bottom of the reaction cup 1110 may be provided with a bottom opening 1111. The top of the reaction cup 1110 may be provided with a top opening 1112. Materials such as resin may enter the reaction cup 1110 through the top opening 1112. Of course, materials such as resin may also enter the reaction cup 1110 through the bottom opening 1111 in advance. The top opening 1112 may also be used as a spare opening to provide users with more options. The reaction cup 1110 may be supported on the support member 1120. The support member 1120 may serve as the base of the reaction vessel 1100. The support member 1120 can be arranged on the shaking device 3000. The feed pipe 1130 can extend downward from the support member 1120. The feed pipe 1130 can be connected to the bottom of the support member 1120 by any suitable means such as welding, bonding or integral molding. A liquid inlet 1131 and an air inlet 1132 can be provided on the side of the feed pipe 1130. Both the liquid inlet 1131 and the air inlet 1132 can be connected to the bottom opening 1111. Specifically, a feed channel 1133 can be provided in the feed pipe 1130. The top of the feed channel 1133 can be connected to the bottom opening 1111. Both the liquid inlet 1131 and the air inlet 1132 can be connected to the lower end of the feed channel 1133. The liquid adding device 2000 can be connected to the liquid inlet 1131 for filling the reaction cup 1110 with piperidine solution. Specifically, the piperidine solution can pass through the liquid adding device 2000, and then can pass through the liquid inlet 1131, the feed channel 1133 and the bottom opening 1111 in sequence, so as to enter the reaction cup 1110. The bubbling device 4000 can be connected to the gas inlet 1132 to bubble nitrogen into the reaction cup 1110. Specifically, the nitrogen can pass through the bubbling device 4000, and then can pass through the gas inlet 1132, the feed channel 1133 and the bottom opening 1111 in sequence, so as to enter the reaction cup 1110.

[0061] In actual application of the reaction container 1100, the piperidine solution and nitrogen can enter the reaction cup 1110 through the bottom opening 1111. In this way, the piperidine solution will rise from the bottom of the reaction cup 1110 during the entry process, so that it will not splash onto the cup wall of the reaction cup 1110, and thus will not cause pollution to the subsequent process. The nitrogen can directly stir the piperidine solution at the bottom of the reaction cup 1110. Moreover, as the nitrogen rises in the reaction cup 1110, the piperidine solution in the reaction cup 1110 can be completely stirred by the nitrogen. In this way, the piperidine solution and the resin can be fully contacted, and the reaction speed of the two can be greatly improved. In addition, there is no need to set a pipeline for introducing the piperidine solution and nitrogen into the bottom of the reaction cup 1110, and the space in the reaction cup 1110 can be fully used for reaction, and the utilization rate of the space in the reaction cup 1110 is high.

[0062] For example, Figure 16-18 As shown, along the lateral direction of the feed tube 1130, the liquid inlet 1131 and the air inlet 1132 can be arranged relatively to each other. The lateral direction can be perpendicular to the extension direction of the feed tube 1130. Generally speaking, the extension direction of the feed tube 1130 can be a vertical direction. Therefore, the lateral direction can be a horizontal direction. With this arrangement, the distance between the liquid inlet 1131 and the air inlet 1132 is relatively far, so that there can be enough space for connecting with the liquid adding device 2000 and the bubbling device 4000. Exemplarily, the liquid inlet 1131 can be arranged on the rear side of the feed tube 1130. The air inlet 1132 can be arranged on the front side of the feed tube 1130. The directional term "rear" used here and below generally refers to the side away from the relevant person. Therefore, the directional term "front" used here and below generally refers to the side close to the relevant person. Figure 2 and 6 Taking the peptide synthesis device shown in as an example, the upper left and lower right in the figure may be the front side.

[0063] For example, Figure 16-18 As shown, along the extension direction of the feed pipe 1130, the liquid inlet 1131 and the air inlet 1132 can be completely staggered. In this way, the liquid inlet 1131 and the air inlet 1132 will not be directly opposite. In this way, when the piperidine solution enters the feed pipe 1130 through the liquid inlet 1131, the piperidine solution will not directly rush to the air inlet 1132, thereby preventing the piperidine solution from flowing out through the air inlet 1132. Similarly, when the nitrogen enters the feed pipe 1130 through the air inlet 1132, the nitrogen will not directly rush to the liquid inlet 1131, thereby preventing the piperidine solution from flowing out through the liquid inlet 1131.

[0064] For example, Figure 16-18As shown, the support member 1120 may include a cup holder 1121 and a sealing ring 1122. The cup holder 1121 may be annular. The cup holder 1121 may be directly or indirectly disposed on the shaking device 3000. The feed pipe 1130 may extend downward from the cup holder 1121. The feed pipe 1130 may be connected to the bottom of the cup holder 1121 by any suitable means such as welding, bonding or integral molding. The reaction cup 1110 may include a large-size section 1113, a small-size section 1114 and a transition section 1115. The large-size section 1113 may be located at the upper end of the reaction cup 1110. The small-size section 1114 may be located at the lower end of the reaction cup 1110. The transition section 1115 may be connected between the upper end and the lower end of the reaction cup 1110. That is, in the vertical direction, the large-size section 1113, the transition section 1115 and the small-size section 1114 may be connected in sequence. In some embodiments, the large-size segment 1113 and the transition segment 1115 may have a smooth transition. The small-size segment 1114 and the transition segment 1115 may have a smooth transition. The bottom opening 1111 may be provided at the bottom of the small-size segment 1114. The top opening 1112 may be provided at the top of the large-size segment 1113. The small-size segment 1114 may be inserted into the cup holder 1121. The transition segment 1115 may be supported on the cup holder 1121. The sealing ring 1122 may surround the small-size segment 1114. Furthermore, the sealing ring 1122 may be clamped between the cup holder 1121 and the small-size segment 1114. In practical applications, the sealing ring 1122 may be pre-placed in the cup holder 1121. When the reaction cup 1110 needs to be installed, the small-size segment 1114 can be first inserted into the sealing ring 1122 in the cup holder 1121, so that the sealing ring 1122 can be clamped between the cup holder 1121 and the small-size segment 1114. With the insertion, when the transition segment 1115 is supported on the cup holder 1121, the reaction cup 1110 is installed in place. With this arrangement, the reaction cup 1110 can be easily installed and disassembled. In this way, after synthesizing the polypeptide, the relevant personnel can directly remove the reaction cup 1110 to facilitate the removal of the polypeptide therein. In addition, the sealing ring 1122 can apply a large friction force to the small-size segment 1114, so that the reaction cup 1110 can be prevented from being easily displaced, ensuring that the reaction cup 1110 is in the expected position. In addition, the sealing ring 1122 can also block the gap between the small-size segment 1114 and the cup holder 1121 to prevent the piperidine solution and / or nitrogen gas passing through the feeding tube 1130 from leaking through the gap due to excessive pressure. The sealing ring 1122 may be any type of sealing ring known in the art or that may appear in the future, including but not limited to a pan seal. The sealing ring 1122 may be made of any material, including but not limited to polytetrafluoroethylene (PTFE).

[0065] For example, Figure 16-18As shown, a filter screen 1140 may be provided in the reaction cup 1110. The filter screen 1140 may be supported on the transition section 1115 by any suitable means such as welding or bonding. The filter screen 1140 may be used to support materials such as resin. The piperidine solution and nitrogen gas passing through the feed pipe 1130 may pass through the filter screen 1140, so as to react with materials such as resin supported on the filter screen 1140. Exemplarily, the filter screen 1140 may adopt a sand core of about 40 meshes, so as to be used to support resin particles with a mesh size of about 100-200 meshes.

[0066] Exemplarily, a one-way valve may be provided in the liquid inlet 1131 and the gas inlet 1132, respectively. The one-way valve in the liquid inlet 1131 may allow the liquid to flow from the outside of the liquid inlet 1131 to the inside of the feed pipe 1130 in a one-way manner. In this way, the piperidine solution will not flow back from the liquid inlet 1131. The one-way valve in the gas inlet 1132 may allow the nitrogen to flow from the outside of the gas inlet 1132 to the inside of the feed pipe 1130 in a one-way manner. In this way, the nitrogen will not flow back from the gas inlet 1132. The one-way valves provided in the liquid inlet 1131 and the gas inlet 1132 may be the same or different.

[0067] For example, Figure 3 As shown, the liquid adding device 2000 may include a liquid injection pump 2400 and a multi-valve 2500. The liquid storage container may include a first liquid storage container for storing a piperidine solution. The liquid inlet of the liquid injection pump 2400 may be used to be connected to the first liquid storage container. Each group of reaction container assemblies 1200 may correspond to a multi-valve 2500. In this way, the liquid inlet of the multi-valve 2500 may be connected to the liquid outlet of the liquid injection pump 2400. The multi-valve 2500 may have a plurality of liquid outlets, and each liquid outlet may be connected to all the liquid inlets 1131 of the corresponding reaction container assembly 1200 in a one-to-one correspondence. In the embodiment shown in the figure, the multi-valve 2500 may adopt a ten-valve, which has ten liquid outlets, so that it can be connected to ten liquid inlets 1131 in a one-to-one correspondence. The liquid injection pump 2400 can fill the piperidine solution into the reaction cup 1110 through the multi-valve 2500.

[0068] For example, Figure 3-4As shown in Figures 19-20, the liquid adding device 2000 may also include a plurality of liquid injection parts 2100, liquid outlet parts 2200 and a plurality of switching valves 2300. The liquid outlet parts 2200 may be arranged in one-to-one correspondence with the plurality of switching valves 2300. Each switching valve 2300 may be arranged between its corresponding liquid injection part 2100 and liquid outlet part 2200. In the embodiment shown in the figure, the liquid injection part 2100 and the switching valve 2300 may include four, respectively. The switching valve 2300 may have a liquid absorption state and a liquid injection state. The switching valve 2300 may switch between the liquid absorption state and the liquid injection state. The switching valve 2300 includes but is not limited to an electromagnetic switching valve, a manual switching valve or a pneumatic switching valve. When the switching valve 2300 is in the liquid absorption state, the corresponding liquid injection part 2100 may be communicated with the liquid storage container. Exemplarily, the liquid storage container may also include a second liquid storage container for storing DMF solution. The liquid injection part 2100 may be communicated with the first liquid storage container and / or the second liquid storage container. Furthermore, when the switching valve 2300 is in the liquid absorption state, the corresponding liquid injection part 2100 can be used to absorb the liquid in the liquid storage container into the liquid injection part 2100. When the switching valve 2300 is in the liquid injection state, the corresponding liquid injection part 2100 can be connected to the liquid outlet part 2200. Furthermore, when the switching valve 2300 is in the liquid injection state, the corresponding liquid injection part 2100 can be used to inject the liquid in the liquid injection part 2100 into the reaction container 1100 below the liquid outlet part 2200 through the liquid outlet part 2200. Specifically, the liquid outlet part 2200 can inject the liquid into the reaction cup 1110 through the top opening 1112. The liquid injection part 2100 includes but is not limited to a pump and a liquid injection container connected to the pump, or any suitable structure such as a negative pressure tank, as long as the above functions can be achieved. The liquid outlet part 2200 includes but is not limited to a liquid outlet tube, a liquid outlet needle or any other suitable structure, as long as the above functions can be achieved.

[0069] In the actual application of the liquid adding device 2000, the switching valve 2300 can be switched to the liquid absorption state. In this way, the liquid injection part 2100 can be communicated with the liquid storage container through the switching valve 2300. The liquid injection part 2100 can extract the liquid in the liquid storage container, so that the liquid can be sucked into the liquid injection part 2100. Then, the switching valve 2300 can be switched to the liquid injection state. In this way, the liquid injection part 2100 can be communicated with the liquid outlet part 2200 through the switching valve 2300. The liquid injection part 2100 can discharge the liquid in it, so that it can be filled into the reaction container 1100 below the liquid outlet part 2200 through the liquid outlet part 2200. The liquid adding device 2000 can realize the automation of filling the liquid into the reaction container 1100, so that manual operation is not required. In this way, the liquid adding device 2000 can avoid labor costs and improve work efficiency. Furthermore, since there is no human factor, the liquid adding device 2000 can accurately control the amount of liquid injected into the reaction vessel 1100 and the timing of the liquid injection, thereby being used to improve the effect of the relevant reaction carried out in the reaction vessel 1100. Furthermore, the multiple liquid injection parts 2100 can increase the liquid injection amount. Compared with the structure of using a large-sized liquid injection part to increase the liquid injection amount, the specification of each of the multiple liquid injection parts 2100 can be relatively small, and its structure is relatively more precise, so that it is easy to achieve accurate control of the liquid injection amount.

[0070] For example, Figure 3-4 As shown, the injection part 2100 may include a motor 2110 and a syringe 2120. The driving end of the motor 2110 may be connected to the syringe 2120. The syringe 2120 may be connected to its corresponding switching valve 2300. The motor 2110 may be used to drive the piston rod of the syringe 2120 to move to perform liquid suction and liquid injection. Specifically, when the switching valve 2300 is in the liquid suction state, the motor 2110 may be used to suck the liquid in the liquid storage container into the syringe 2120. When the switching valve 2300 is in the liquid injection state, the motor 2110 may be used to inject the liquid in the syringe 2120 into the reaction vessel 1100 through the liquid outlet 2200. The motor 2110 may adopt various types of motors known in the art or that may appear in the future, including but not limited to a servo motor or a stepper motor. In this way, the structure of the injection part 2100 is simple, and the precision of liquid suction and liquid injection is high, so that the amount of liquid injection can be accurately controlled.

[0071] For example, Figure 3-4As shown, the liquid adding device 2000 may further include a mounting plate 2600. The mounting plate 2600 may serve as a base for the liquid adding device 2000. The mounting plate 2600 may be connected to the mounting platform 9111. The liquid injection piece 2100 and the switching valve 2300 may be arranged at intervals on the mounting plate 2600. In this arrangement, the liquid injection piece 2100 and the corresponding switching valve 2300 may be adjacent to each other, thereby facilitating connection. Exemplarily, the mounting plate 2600 may be a long strip extending in a straight line direction. The liquid injection piece 2100 and the switching valve 2300 may be arranged at intervals on the mounting plate 2600 in the straight line direction.

[0072] For example, Figure 19-20 As shown, the liquid adding device 2000 may also include a floating mechanism 2700. The liquid outlet 2200 may be arranged on the floating mechanism 2700. By arranging the floating mechanism 2700, the liquid outlet 2200 may be lifted and lowered in the vertical direction. Since the reaction vessel 1100 is located below the liquid outlet 2200, when the reaction vessel 1100 and the liquid outlet 2200 contact, the liquid outlet 2200 may rise under the top of the reaction vessel 1100. Moreover, when the liquid outlet 2200 is separated from the reaction vessel 1100, the liquid outlet 2200 may descend under the action of gravity, thereby returning to the initial position. When the liquid outlet 2200 rises to a predetermined position, the floating mechanism 2700 may control the liquid adding device 2000 to stop. In this way, in actual application, when the liquid outlet 2200 contacts the reaction vessel 1100, it indicates that the liquid outlet 2200 and the reaction vessel 1100 are not aligned. If the liquid adding device 2000 still works normally at this time, the liquid passing through the liquid outlet 2200 may flow outside the reaction vessel 1100, thereby causing a safety accident. By setting the floating mechanism 2700, when the liquid outlet 2200 rises to a predetermined position, the floating mechanism 2700 can control the liquid adding device 2000 to shut down. In this way, the liquid cannot flow out through the liquid outlet 2200, thereby not causing a safety accident. The shutdown of the liquid adding device 2000 may include the shutdown of the liquid injection part 2100 and / or the switching valve 2300 switching to the liquid suction state, etc. Exemplarily, a button may be provided on the floating mechanism 2700. When the liquid outlet 2200 rises to a predetermined position, the liquid outlet 2200 will push against the button, thereby controlling the liquid adding device 2000 to shut down.

[0073] For example, Figure 19-20As shown, the floating mechanism 2700 may include a floating body 2710, a floating member 2720, a floating detector 2730 and a first controller (not shown). The floating member 2720 is arranged on the floating body 2710 in a manner that it can be raised and lowered. The liquid outlet 2200 may be arranged on the floating member 2720. The floating member 2720 may also be provided with a sensing sheet 2721. In this way, under the pressure of the reaction container 1100, the liquid outlet 2200 may drive the floating member 2720 and the sensing sheet 2721 to rise relative to the floating body 2710. When the liquid outlet 2200 is separated from the reaction container 1100, the liquid outlet 2200 may drive the floating member 2720 and the sensing sheet 2721 to fall relative to the floating body 2710 under the action of gravity. When the liquid outlet 2200 rises to a predetermined position, the floating detector 2730 may detect the sensing sheet 2721 and send a control signal to the first controller. The first controller may be used to control the liquid adding device 2000 to stop based on the control signal. The floating detector 2730 includes but is not limited to a laser sensor or an infrared sensor, as long as the above functions can be realized. The first controller can be constructed using electronic components such as a timer, a comparator, a register, a digital logic circuit, or can be implemented using a processor chip such as a single chip microcomputer, a microprocessor, a programmable logic controller (PLC), a digital signal processor (DSP), a field programmable gate array (FPGA), a programmable logic array (PLA), an application specific integrated circuit (ASIC) and its peripheral circuits. The floating mechanism 2700 has a relatively simple structure, low manufacturing cost, and stable performance.

[0074] For example, Figure 19-20 As shown, the floating detector 2730 may include a transmitter 2731 and a receiver 2732. The transmitter 2731 and the receiver 2732 may be arranged at intervals. When the liquid outlet 2200 rises to a predetermined position, the sensor sheet 2721 may be located between the transmitter 2731 and the receiver 2732. In this way, the sensor sheet 2721 may block the receiver 2732 from receiving the light emitted by the transmitter 2731. At this time, the floating detector 2730 may send a control signal to the first controller. The first controller may be used to control the liquid adding device 2000 to shut down based on the control signal. With such an arrangement, the structure of the floating detector 2730 is relatively simple, and it will not contact the sensor sheet 2721, so that interference and other problems will not occur.

[0075] For example, Figure 19-20As shown, the liquid outlet member 2200 may include a plurality of first liquid outlet needles 2210, such as two, three or more. In the embodiment shown in the figure, the first liquid outlet needles 2210 may include six. The plurality of first liquid outlet needles 2210 may be arranged in any suitable manner. The reaction container 1100 may be disposed below the plurality of first liquid outlet needles 2210. The plurality of first liquid outlet needles 2210 may be used to inject liquid into the same reaction container 1100, thereby increasing the injection amount. Figure 21-22 As shown, one or more first liquid outlet holes 2211 may be provided on the side wall of each first liquid outlet needle 2210. The first liquid outlet hole 2211 may be a circular hole or a hole of any other suitable shape. The first liquid outlet hole 2211 may be tilted downward. When the switching valve 2300 is in the liquid injection state, the corresponding liquid injection part 2100 may be used to inject the DMF solution in the liquid injection part 2100 into the reaction vessel 1100 below the first liquid outlet needle 2210 through the first liquid outlet hole 2211. Since the first liquid outlet hole 2211 is tilted downward, the DMF solution passing through it may flow out tilted downward, so that it may contact and flow downward along the inner side wall of the reaction vessel 1100. With such arrangement, the DMF solution may be used to clean the inner side wall of the reaction vessel 1100. The DMF solution may clean the piperidine solution on the inner side wall of the reaction vessel 1100, thereby improving the cleanliness of the reaction vessel 1100, and thus facilitating the subsequent process.

[0076] For example, Figure 21-22 As shown, along the circumferential direction of the first liquid outlet needle 2210, a plurality of first liquid outlet holes 2211, such as two, three or more, may be arranged at intervals on the side wall of the first liquid outlet needle 2210. In this way, the DMF solution passing through the first liquid outlet holes 2211 can clean multiple positions of the inner side wall of the reaction container 1100 along the circumferential direction, thereby improving the cleaning effect.

[0077] For example, Figure 21-22 As shown, along the extension direction of the first liquid outlet needle 2210, a plurality of first liquid outlet holes 2211, such as two, three or more, may be arranged at intervals on the side wall of the first liquid outlet needle 2210. In this way, the DMF solution passing through the first liquid outlet holes 2211 can clean multiple positions of the inner side wall of the reaction container 1100 along the extension direction, thereby improving the cleaning effect.

[0078] For example, Figure 19-20As shown in Figures 23 and 23, the liquid outlet part 2200 may also include a plurality of second liquid outlet needles 2220, for example, two, three or more. In the embodiment shown in the figure, the second liquid outlet needles 2220 may include three. The plurality of second liquid outlet needles 2220 may be arranged in any suitable manner. The reaction container 1100 may be disposed below the plurality of second liquid outlet needles 2220. The plurality of second liquid outlet needles 2220 may be used to inject liquid into the same reaction container 1100, thereby increasing the injection amount. Fig.23 As shown, each second liquid outlet needle 2220 may include a needle tube 2221 and an umbrella cover 2222. The umbrella cover 2222 may extend obliquely downward from the side wall of the needle tube 2221. A second liquid outlet hole 2223 may be provided on the needle tube 2221. The umbrella cover 2222 may be provided to cover and protrude downward from the second liquid outlet hole 2223. When the switching valve 2300 is in the liquid injection state, the corresponding liquid injection part 2100 may be used to inject the piperidine solution in the liquid injection part 2100 into the reaction container 1100 below the second liquid outlet needle 2220 through the second liquid outlet needle 2220. Specifically, the piperidine solution may pass through the second liquid outlet hole 2223 of the needle tube 2221, and then may flow downward along the inner side wall of the umbrella cover 2222. With such a configuration, the piperidine solution can enter the reaction vessel 1100 in a shower-like state through the second liquid outlet needle 2220, thereby reducing impact and inhibiting the piperidine solution from splashing onto the inner wall of the reaction vessel 1100, thereby ensuring the cleanliness of the reaction vessel 1100. The process requirement of the piperidine solution is that it cannot be on the inner wall of the reaction vessel 1100, thereby preventing contamination of subsequent processes.

[0079] For example, Figure 19-20As shown, the liquid outlet 2200 may also include a liquid receiving tank driver 2230 and a liquid receiving tank 2240. The driving end of the liquid receiving tank driver 2230 may be connected to the liquid receiving tank 2240. Driven by the liquid receiving tank driver 2230, the liquid receiving tank 2240 may move between the liquid receiving position and the interval position in the horizontal direction. The liquid receiving tank driver 2230 includes but is not limited to a motor, a cylinder or a hydraulic cylinder. When the liquid receiving tank 2240 is located at the liquid receiving position, it may be located below the umbrella cover 2222. In this way, the liquid receiving tank 2240 may contain droplets dripping from the umbrella cover 2222 to prevent the droplets from falling into the reaction vessel 1100 below. The second liquid outlet needle 2220 may be used as a backup liquid outlet solution, and relevant personnel may choose to fill the piperidine solution from the bottom opening 1111 or the top opening 1112 of the reaction vessel 1100. The second liquid outlet needle 2220 is usually not used. To this end, by placing the liquid receiving groove 2240 at the liquid receiving position, it is possible to prevent the droplets dripping from the second liquid outlet needle 2220 from affecting the relevant processes. When the liquid receiving groove 2240 is located at the spacing position, it can be spaced apart from the umbrella cover 2222 in the horizontal direction. In this way, the liquid injection part 2100 can be used to inject the piperidine solution in the liquid injection part 2100 into the reaction container 1100 below the second liquid outlet needle 2220 through the second liquid outlet needle 2220, and the liquid receiving groove 2240 will not cause any obstruction.

[0080] Exemplarily, the liquid outlet part 2200 may include a plurality of first liquid outlet needles 2210 and a plurality of second liquid outlet needles 2220. The plurality of liquid injection parts 2100 may be connected to all the first liquid outlet needles 2210 and the second liquid outlet needles 2220 through the switching valve 2300; or a part of the liquid injection parts 2100 may be connected to the first liquid outlet needles 2210 through the switching valve 2300, and another part of the liquid injection parts 2100 may be connected to the second liquid outlet needles 2220 through the switching valve 2300.

[0081] For example, Figure 19-20As shown, the liquid outlet member 2200 may further include a second liquid outlet needle driver 2250. The driving end of the second liquid outlet needle driver 2250 may be connected to the second liquid outlet needle 2220 to drive the second liquid outlet needle 2220 to rise and fall. The second liquid outlet needle driver 2250 includes but is not limited to a motor, a cylinder or a hydraulic cylinder. The second liquid outlet needle driver 2250 may drive the second liquid outlet needle 2220 to extend downward into the reaction vessel 1100, thereby reducing the distance between the umbrella cover 2222 and the liquid surface in the reaction vessel 1100, thereby reducing the impact as much as possible and inhibiting the piperidine solution from splashing onto the inner wall of the reaction vessel 1100. When the injection is completed, the second liquid outlet needle driver 2250 may drive the second liquid outlet needle 2220 to extend upward outside the reaction vessel 1100. In the embodiment where the liquid outlet member 2200 includes the liquid receiving tank driving member 2230 and the liquid receiving tank 2240, when the liquid receiving tank 2240 is located at the spacing position, the second liquid outlet needle driving member 2250 can be used to drive the second liquid outlet needle 2220 to rise and fall. In the embodiment where the liquid adding device 2000 includes the floating mechanism 2700, during the process where the second liquid outlet needle driving member 2250 drives the second liquid outlet needle 2220 to descend, if the second liquid outlet needle 2220 contacts the reaction container 1100 due to misalignment with the reaction container 1100, when the liquid outlet member 2200 rises to a predetermined position under the pressure of the reaction container 1100, the floating mechanism 2700 can control the liquid adding device 2000 to stop.

[0082] For example, Figure 2-3As shown in Figures 19-20, the liquid adding device 2000 may also include a first horizontal driving member 2810, a second horizontal driving member 2820, and a vertical driving member 2830. The driving end of the vertical driving member 2830 may be connected to the liquid outlet member 2200 to drive the liquid outlet member 2200 to rise and fall in the vertical direction. The driving end of the second horizontal driving member 2820 may be connected to the main body of the vertical driving member 2830 to drive the vertical driving member 2830 and the liquid outlet member 2200 to move in the second horizontal direction. The driving end of the first horizontal driving member 2810 may be connected to the main body of the second horizontal driving member 2820 to drive the second horizontal driving member 2820, the vertical driving member 2830, and the liquid outlet member 2200 to move in the first horizontal direction. The first horizontal direction, the second horizontal direction, and the vertical direction may be perpendicular to each other. The first horizontal driving member 2810, the second horizontal driving member 2820 and the vertical driving member 2830 can form a three-axis driving mechanism, so that the liquid outlet 2200 can be moved to more positions. The first horizontal driving member 2810, the second horizontal driving member 2820 and the vertical driving member 2830 include but are not limited to motors, cylinders or hydraulic cylinders. In practical applications, the first horizontal driving member 2810 and the second horizontal driving member 2820 can drive the vertical driving member 2830 and the liquid outlet 2200 to move above the reaction vessel 1100. Then, the vertical driving member 2830 can drive the liquid outlet 2200 to descend, so that it can be close to the reaction vessel 1100 for easy injection. In the embodiment where the liquid outlet member 2200 includes the second liquid outlet needle 2220 and the second liquid outlet needle driving member 2250, the vertical driving member 2830 can drive the liquid outlet member 2200 to descend to approach the reaction container 1100, and then the second liquid outlet needle driving member 2250 can drive the second liquid outlet needle 2220 to further descend to extend into the reaction container 1100. Exemplarily, the liquid adding device 2000 may further include a liquid discharge container 2900. The liquid discharge container 2900 may be disposed below the path where the liquid outlet member 2200 moves. In this way, the first horizontal driving member 2810 and the second horizontal driving member 2820 may drive the liquid outlet member 2200 to move above the liquid discharge container 2900. Then, the liquid injection member 2100 may discharge liquid into the liquid discharge container 2900 through the liquid outlet member 2200, so that the air in the pipeline between the liquid injection member 2100 and the liquid outlet member 2200 may be discharged. After the liquid discharge is completed, the first horizontal driving member 2810 and the second horizontal driving member 2820 can drive the vertical driving member 2830 and the liquid outlet member 2200 to move to the top of the reaction container 1100 for subsequent liquid filling operations. Since the air has been discharged, the liquid outlet member 2200 can fill the reaction container 1100 with a higher precision, thereby improving the effect of the relevant reaction performed in the reaction container 1100.

[0083] For example, Figure 6-7As shown, the polypeptide synthesis device may further include a cover opening and closing device 7000. The cover opening and closing device 7000 may be directly or indirectly arranged on the shaking device 3000. The cover opening and closing device 7000 may include a cover opening and closing drive member 7100 and a cover plate 7200. The driving end of the cover opening and closing drive member 7100 may be connected to the cover plate 7200 to drive the cover plate 7200 to move between the cover opening position and the cover closing position in the vertical direction. When the cover plate 7200 is in the cover opening position, it may be spaced apart from the top opening 1112. In this arrangement, materials such as resin and amino acids may be added to the reaction vessel 1100 through the top opening 1112. When the cover plate 7200 is in the cover closing position, it may be blocked to the top opening 1112. When the cover plate 7200 is in the cover closing position, the shaking device 3000 may drive the reaction vessel 1100 and the cover opening and closing device 7000 that covers it to swing synchronously. In this way, the cover opening and closing device 7000 can prevent the liquid in the reaction container 1100 from spilling out through the top opening 1112 during the shaking process. The cover opening and closing driving member 7100 includes but is not limited to a motor or a cylinder.

[0084] For example, Figure 6-7 As shown, each group of reaction container assemblies 1200 may correspond to one opening and closing cover device 7000. Thus, the opening and closing cover device 7000 may be used to cover each top opening 1112 in the corresponding reaction container assembly 1200.

[0085] For example, Figure 6-7 As shown, the cover opening and closing device 7000 may also include a transverse drive member 7300. The driving end of the transverse drive member 7300 may be connected to the main body of the cover opening and closing drive member 7100. In this way, the transverse drive member 7300 may drive the cover opening and closing drive member 7100 and the cover plate 7200 to move synchronously. When the cover plate 7200 is in the cover opening position, the transverse drive member 7300 may be used to drive the cover opening and closing drive member 7100 and the cover plate 7200 to move between the staggered position and the aligned position in the horizontal direction. When the cover plate 7200 is in the staggered position, it may be staggered with the top opening 1112 in the horizontal direction. In this way, it is more convenient to add materials into the reaction vessel 1100 through the top opening 1112. When the cover plate 7200 is in the aligned position, it may be directly above the top opening 1112. In this way, the cover opening and closing drive member 7100 may drive the cover plate 7200 to move between the cover opening position and the cover closing position in the vertical direction. The transverse driving member 7300 includes but is not limited to a motor or a cylinder.

[0086] For example, Figure 6-7As shown, an elastic layer 7210 made of an elastic material (such as rubber or silicone) may be provided on the cover plate 7200. When the cover plate 7200 is in the closed position, the elastic layer 7210 may be sealed to the top opening 1112. By providing the elastic layer 7210, the sealing between the cover plate 7200 and the reaction container 1100 may be improved, thereby further preventing the liquid in the reaction container 1100 from spilling through the top opening 1112 during the shaking process.

[0087] For example, Figure 16-17 As shown, the bottom of the reaction container 1100 may be provided with a drain port 1134. Specifically, the drain port 1134 may be provided at the bottom of the feed pipe 1130. The bottom of the feed channel 1133 may be connected to the drain port 1134. Figure 2-3 As shown in FIGS. 5-7 , the polypeptide synthesis device may further include a liquid discharge device 6000 . The liquid discharge device 6000 may include a positive pressure liquid discharge component 6100 and a negative pressure liquid discharge component 6200 .

[0088] The positive pressure discharge assembly 6100 can be used to inject gas (e.g., nitrogen) into the reaction vessel 1100 from the top opening 1112 to apply pressure to the reaction vessel 1100. The positive pressure discharge assembly 6100 includes, but is not limited to, an air pump assembly or a fan assembly, as long as gas can be injected into the reaction vessel 1100 from the top opening 1112 to apply pressure to the reaction vessel 1100. The negative pressure discharge assembly 6200 can be connected to the drain port 1134 to extract the liquid in the reaction vessel 1100 through the drain port 1134. The negative pressure discharge assembly 6200 includes, but is not limited to, a liquid pump assembly or a negative pressure tank assembly, as long as the liquid in the reaction vessel 1100 can be extracted through the drain port 1134. Specifically, the negative pressure discharge assembly 6200 can form a negative pressure. Under the action of the negative pressure, the liquid in the reaction vessel 1100 can be discharged through the drain port 1134.

[0089] In actual application of the peptide synthesis device, the negative pressure drainage component 6200 can extract the liquid in the reaction container 1100 through the drainage port 1134, so that the expected material is left in the reaction container 1100. At the same time, the positive pressure drainage component 6100 can inject gas into the reaction container 1100 from the top opening 1112 to apply pressure to the reaction container 1100. The pressure will push the liquid in the reaction container 1100, so that the liquid can be accelerated to be discharged through the drainage port 1134. In this way, the discharge speed of the liquid in the reaction container 1100 is faster, and the working efficiency of the peptide synthesis device is higher, so that the flux of peptide synthesis can be improved. In addition, the pressure will also push the residual liquid on the inner wall of the reaction container 1100 to flow downward, so that the liquid is discharged through the drainage port 1134. In this way, by setting the positive pressure drainage component 6100, the residual liquid in the reaction container 1100 can be reduced to reduce the impact on subsequent processes.

[0090] For example, Figure 7-9 As shown, the discharge device 6000 may further include a discharge pipeline 6300 and a clamping tube assembly 6400. The discharge pipeline 6300 may be connected between the negative pressure discharge assembly 6200 and the discharge port 1134. The discharge pipeline 6300 may adopt any suitable pipeline, for example, it may be a polymer pipeline. In this way, under the action of the negative pressure discharge assembly 6200, the liquid in the reaction vessel 1100 may be discharged to the negative pressure discharge assembly 6200 through the discharge port 1134 and the discharge pipeline 6300 in turn. The clamping tube assembly 6400 may be used to clamp the discharge pipeline 6300 so that the discharge pipeline 6300 is cut off. Specifically, when the reaction is carried out in the reaction vessel 1100, the clamping tube assembly 6400 may clamp the discharge pipeline 6300 so that the discharge pipeline 6300 is cut off. In this way, the liquid in the reaction vessel 1100 cannot be discharged through the discharge port 1134. When the reaction is completed, the clamping assembly 6400 can release the drainage pipeline 6300 to make the drainage pipeline 6300 conductive. In this way, under the action of the negative pressure drainage assembly 6200, the liquid in the reaction vessel 1100 can be discharged to the negative pressure drainage assembly 6200 through the drainage port 1134 and the drainage pipeline 6300 in turn. The clamping assembly 6400 includes but is not limited to a clamping valve assembly. Compared with the structure of setting a stop valve on the drainage pipeline 6300, the clamping assembly 6400 can be free from contacting the liquid in the drainage pipeline 6300, thereby not causing pollution. In addition, the clamping assembly 6400 does not cause the drainage pipeline 6300 to increase the interface, so the problem of leakage can be avoided.

[0091] Exemplarily, the drain pipe 6300 and the clamping tube assembly 6400 may be arranged in a one-to-one correspondence. Moreover, each group of reaction container assemblies 1200 may correspond to a drain pipe 6300 and a clamping tube assembly 6400. The drain pipe 6300 may be connected to all drain ports 1134 of the corresponding reaction container assembly 1200.

[0092] For example, Figure 7-9 As shown, the clamping tube assembly 6400 may include a clamping tube body 6410, a clamping tube driving member 6420 and a pusher 6430. A resisting surface (not shown due to angle problems) may be provided on the clamping tube body 6410. The driving end of the clamping tube driving member 6420 may be connected to the pusher 6430 to drive the pusher 6430 to move between the cut-off position and the conducting position. Wherein, when the pusher 6430 is in the cut-off position, it can clamp the discharge pipeline 6300 with the resisting surface of the clamping tube body 6410 to cut off the discharge pipeline 6300. When the pusher 6430 is in the conducting position, it can be separated from the discharge pipeline 6300 to conduct the discharge pipeline 6300. The clamping tube driving member 6420 includes but is not limited to a motor or a cylinder. Exemplarily, the pusher 6430 may be located directly below the resisting surface of the clamping tube body 6410. The clamping tube driving member 6420 can drive the pushing member 6430 to move upward to the cut-off position, and drive the pushing member 6430 to move downward to the conducting position. Thus, when the pushing member 6430 moves downward to the conducting position, the discharge pipe 6300 can restore its shape by itself under the action of gravity to be in the conducting state.

[0093] For example, Figure 8-9 As shown, a pair of limiting holes 6411 may be further provided on the clamp body 6410. Along the extension direction of the drainage pipeline 6300, the pair of limiting holes 6411 may be located on both sides of the abutment surface of the clamp body 6410. The drainage pipeline 6300 may pass through the pair of limiting holes 6411 in sequence. The size of each of the pair of limiting holes 6411 may be adapted to the drainage pipeline 6300. The pair of limiting holes 6411 may support and limit the drainage pipeline 6300, so that the flexible drainage pipeline 6300 may be in a desired position, so that the pusher 6430 and the abutment surface of the clamp body 6410 can clamp the drainage pipeline 6300.

[0094] For example, Figure 8-9As shown, the pusher 6430 may have a pusher surface 6431 facing the drain pipe 6300. The pusher surface 6431 may clamp the drain pipe 6300 with the abutment surface of the clamp body 6410. The pusher surface 6431 may be an arcuate surface protruding toward the drain pipe 6300. With such a configuration, the contact area between the arcuate surface and the drain pipe 6300 is small, thereby increasing the clamping pressure and further increasing the cutoff effect on the drain pipe 6300. In addition, the arcuate surface is not easy to damage the drain pipe 6300.

[0095] Exemplarily, the surface roughness of the pusher surface 6431 and the abutment surface of the clamp tube body 6410 may be relatively low (eg, may be a mirror surface), thereby avoiding damage to the drainage pipeline 6300 .

[0096] For example, Figure 8-9 As shown, the clamping tube drive 6420 may include a cylinder. The cylinder may be various types of cylinders known in the art or that may appear in the future, including but not limited to a three-axis cylinder. The piston rod of the cylinder may be connected to the pusher 6430. Compared with the electric clamping tube drive, the cylinder generates less heat, thereby meeting the explosion-proof requirements.

[0097] For example, Figure 6-9 As shown, the drainage pipeline 6300 may include a plurality of shunt drainage pipelines 6310. The plurality of shunt drainage pipelines 6310 are arranged one-to-one in correspondence with all the reaction containers 1100 of the corresponding reaction container assembly 1200. The shunt drainage pipeline 6310 may be connected between the negative pressure drainage assembly 6200 and the corresponding drainage port 1134. In this way, under the action of the negative pressure drainage assembly 6200, the liquid in the reaction container 1100 may be discharged to the negative pressure drainage assembly 6200 through its drainage port 1134 and the shunt drainage pipeline 6310 corresponding to the drainage port 1134 in turn. The clamp assembly 6400 may be used to clamp all the corresponding shunt drainage pipelines 6310 so that the shunt drainage pipeline 6310 is cut off. In the embodiment in which the limiting hole 6411 is provided on the clamp body 6410, each shunt drainage pipeline 6310 may pass through a pair of limiting holes 6411 in turn. The size of each of the pair of limiting holes 6411 can be adapted to the shunt drainage pipeline 6310. In this arrangement, since the clamp assembly 6400 can be used to clamp all of its corresponding shunt drainage pipelines 6310, the liquid discharged through the drain port 1134 will not enter other reaction vessels 1100 in the reaction vessel assembly 1200 where it is located.

[0098] For example, Figure 8-9As shown, the drain line 6300 may also include a manifold 6320. The drain line 6300 may also include a confluent drain line (not shown). A plurality of shunt drain lines 6310, a manifold 6320, a confluent drain line and a negative pressure drain assembly 6200 may be connected in sequence. That is to say, under the effect of the negative pressure drain assembly 6200, the liquid in the reaction vessel 1100 may be discharged to the negative pressure drain assembly 6200 through its drain port 1134, the shunt drain line 6310 corresponding to the drain port 1134, the manifold 6320 and the confluent drain line in sequence. By arranging the manifold 6320 and the confluent drain line, the number of pipelines can be reduced, thereby facilitating wiring. In addition, the number of interfaces (i.e., the following liquid inlet 6212) connected to the drain line 6300 may be reduced on the negative pressure drain assembly 6200, thereby simplifying the structure.

[0099] For example, Figure 5 and 24 As shown, the negative pressure drainage assembly 6200 may include a negative pressure tank 6210 and a vacuum pump 6220. The negative pressure tank 6210 may be provided with a negative pressure port 6211 and a liquid inlet 6212. The negative pressure port 6211 and the liquid inlet 6212 may be interconnected. The vacuum pump 6220 may be connected to the negative pressure port 6211 to evacuate the negative pressure tank 6210. The vacuum pump 6220 may adopt various types of vacuum pumps known in the art or that may appear in the future, including but not limited to a diaphragm vacuum pump. The liquid inlet 6212 may be connected to the drainage port 1134. In an embodiment where the polypeptide synthesis device includes a drainage pipeline 6300, the drainage pipeline 6300 may be connected between the drainage port 1134 and the liquid inlet 6212. Depending on the number of groups of the reaction vessel assembly 1200, the number of liquid inlets 6212 includes but is not limited to one, two, three or more. Each liquid inlet 6212 may be used to connect one or more drainage pipelines 6300. The vacuum pump 6220 can evacuate the negative pressure tank 6210, thereby forming a negative pressure environment in the negative pressure tank 6210. In this way, under the effect of negative pressure, the liquid in the reaction container 1100 can be drawn into the negative pressure tank 6210 through the drain port 1134. The negative pressure port 6211 and the liquid inlet 6212 can both be arranged at the top of the negative pressure tank 6210. In this way, the liquid in the negative pressure tank 6210 will not flow back out through the negative pressure port 6211 and the liquid inlet 6212.

[0100] Exemplarily, a one-way valve may be further provided in the discharge port 1134. That is, the one-way valve may allow the liquid to pass through the discharge port 1134 to one-way conduction outside the reaction vessel 1100. Specifically, the one-way valve may be provided in the feed channel 1133 of the feed pipe 1130. By providing the one-way valve, the liquid may be prevented from flowing back into the reaction vessel 1100 through the discharge port 1134.

[0101] For example, Figure 5 As shown, the negative pressure tank 6210 and the vacuum pump 6220 can be arranged in the cabinet 9110 of the peptide synthesis device. In this way, the liquid can also be discharged by gravity, thereby speeding up the discharge speed.

[0102] Exemplarily, since the liquid can be stored in the negative pressure tank 6210, the negative pressure tank 6210 can be made of corrosion-resistant materials. Exemplarily, the negative pressure tank 6210 can be made of 304 stainless steel or 316 stainless steel. The negative pressure tank 6210 can withstand at least one atmosphere of pressure. The volume of the negative pressure tank 6210 can be about 36L. In the process of manufacturing the negative pressure tank 6210, the inside and outside of the negative pressure tank 6210 can be cleaned by a passivation cleaning solution.

[0103] For example, Figure 5 and 24 As shown, the negative pressure tank 6210 may also be provided with a liquid outlet (not shown due to angle problems). The liquid outlet may be connected to the negative pressure port 6211 and the liquid inlet 6212. The liquid outlet may be used to connect to a liquid pipeline. The liquid in the negative pressure tank 6210 may be discharged through the liquid outlet and the liquid pipeline in turn. The liquid outlet may generally be located at the bottom of the negative pressure tank 6210 so that all the liquid may be discharged. A switch valve 6250 may be provided on the liquid outlet to control the discharge of the liquid.

[0104] For example, Figure 5 and 24 As shown, a liquid level detector may also be provided on the negative pressure tank 6210. A liquid stop valve 6230 may be provided on the liquid pipeline. The negative pressure liquid discharge assembly 6200 may also include a second controller. The second controller may refer to the first controller. The second controller and the first controller may be provided independently or integrated together. The second controller may be electrically connected to the liquid level detector. The liquid level detector may be used to detect the liquid level in the negative pressure tank 6210. When the liquid level in the negative pressure tank 6210 reaches the expected liquid level, the liquid level detector may send a signal to the second controller. The second controller may be used to control the liquid stop valve 6230 to be turned on based on the signal, and to control the vacuum pump 6220 to shut down. After the vacuum pump 6220 is shut down, the liquid will not continue to enter the negative pressure tank 6210. After the liquid stop valve 6230 is turned on, the liquid may be discharged through the liquid pipeline. In this way, the liquid in the negative pressure tank 6210 may be automatically discharged to avoid excessive liquid in the negative pressure tank 6210, which may cause dangerous accidents. When the drainage is completed (for example, when the liquid level detector detects that the liquid level in the negative pressure tank 6210 reaches the lowest level), the second controller can control the liquid shut-off valve 6230 to be closed and control the vacuum pump 6220 to start, so that the liquid can continue to enter the negative pressure tank 6210.

[0105] For example, Figure 5 and 24 As shown, a liquid level pipeline 6240 may be further provided on the negative pressure tank 6210. One end of the liquid level pipeline 6240 may be connected to the bottom of the negative pressure tank 6210. The other end of the liquid level pipeline 6240 may be connected to the top of the negative pressure tank 6210. A transparent window 6241 may be provided on the liquid level pipeline 6240. Exemplarily, the transparent window 6241 may be a transparent pipe section made of transparent Teflon or an equivalent corrosion-resistant material. The bottom of the transparent window 6241 is not higher than the bottom of the negative pressure tank 6210. The top of the transparent window 6241 is not lower than the top of the negative pressure tank 6210. In this way, relevant personnel can observe the liquid level of the negative pressure tank 6210 through the transparent window 6241.

[0106] For example, Figure 5 and 24 As shown, a positive pressure port 6213 may also be provided on the negative pressure tank 6210. The positive pressure port 6213 may be connected to the negative pressure port 6211 and the liquid inlet 6212. The positive pressure port 6213 may be used to connect to a second gas source. In this way, when the negative pressure tank 6210 needs to discharge liquid through the liquid outlet, the second gas source may inject gas (e.g., nitrogen) into the negative pressure tank 6210 through the positive pressure port 6213, thereby accelerating the discharge of the negative pressure tank 6210. Exemplarily, the positive pressure port 6213 may be provided at the top of the negative pressure tank 6210. In this way, the liquid in the negative pressure tank 6210 will not flow back out through the positive pressure port 6213.

[0107] For example, Figure 5 and 24 As shown, the negative pressure tank 6210 may also be provided with a spare port 6214. The spare port 6214 may be blocked with a plug.

[0108] For example, Figure 6-7As shown, each group of reaction vessel assembly 1200 can correspond to a positive pressure discharge assembly 6100. Positive pressure discharge assembly 6100 may include three-way joint 6110 and connecting pipe (not shown). Three-way joint 6110 can be arranged one by one with all reaction vessels 1100 in its corresponding reaction vessel assembly 1200. The first end of three-way joint 6110 can be one by one towards all top openings 1112 in its corresponding reaction vessel assembly 1200. Along the arrangement direction of three-way joint 6110, the second end of first three-way joint 6110 can be used to be connected to the third gas source. The third end of the last three-way joint 6110 can be blocked with a plug to prevent gas from flowing out. The connecting pipe can be connected between the second end and the third end of two adjacent three-way joints 6110. In this way, the third gas source can supply gas (e.g., nitrogen) so that the gas can flow from the first three-way connector 6110 to the last three-way connector 6110 along the arrangement direction of the three-way connectors 6110, and the gas is injected into the reaction container 1100 through the first end of each three-way connector 6110. In this way, the structure of the positive pressure drainage assembly 6100 is relatively simple. The third gas source, the second gas source, and the first gas source can be the same gas source or different gas sources.

[0109] For example, Figure 6-7 As shown, the positive pressure drainage assembly 6100 may also be connected to an air pressure detector 6120. The air pressure detector 6120 includes but is not limited to a barometer or an air pressure sensor. The air pressure detector 6120 may be used to detect the pressure of the gas in the positive pressure drainage assembly 6100.

[0110] The positive pressure liquid discharge assembly 6100 can be arranged on the cover plate 7200. Specifically, the three-way joint 6110 can be arranged on the cover plate 7200. When the cover plate 7200 is in the closed state, the positive pressure liquid discharge assembly 6100 can inject gas from the top opening 1112 into the reaction vessel 1100. By blocking the top opening 1112 by the cover plate 7200, the pressure in the reaction vessel 1100 can be quickly increased, which can be conducive to forming a positive pressure environment, and then the effect of liquid discharge can be improved. By providing the elastic layer 7210, the sealing between the cover plate 7200 and the reaction vessel 1100 can be improved, which can further be conducive to forming a positive pressure environment, and then the effect of liquid discharge can be improved.

[0111] For example, Figure 6-7As shown in 10-11, the shaking device 3000 may include a shaking drive assembly 3100, a rotating disk 3200, a shaking plate 3300 and an eccentric 3400. The driving end of the shaking drive assembly 3100 may be connected to the rotating disk 3200 to drive the rotating disk 3200 to rotate around the rotation axis. Exemplarily, the rotation axis may be the axis of the driving end of the shaking drive assembly 3100. The reaction vessel 1100 may be arranged on the shaking plate 3300. The eccentric 3400 may be connected between the rotating disk 3200 and the shaking plate 3300. The eccentric 3400 may not be coaxial with the rotation axis. In other words, the eccentric 3400 may be eccentrically arranged on the rotating disk 3200. The eccentric 3400 may adopt a projection, a long rod or any other suitable structure. Driven by the shaking drive assembly 3100, the eccentric member 3400 can drive the shaking plate 3300 and the reaction vessel 1100 to swing along the first linear direction XX. The first linear direction XX can be perpendicular to the axis of rotation. Compared with the pendulum-like swing, the reaction vessel 1100 only swings in one direction (i.e., the first linear direction XX), so the liquid in the reaction vessel 1100 is not easily thrown out. In this way, the shaking device 3000 can reduce the possibility of the liquid in the reaction vessel 1100 being thrown out, the safety and reliability of the shaking device 3000 are higher, and the effect of the reaction can be improved. In addition, the structure of the shaking device 3000 is more compact.

[0112] For example, Figure 10-11 As shown, the shaking device 3000 may also include a connecting plate 3500. A long slot 3510 may be provided on the connecting plate 3500. The long slot 3510 may extend along the second linear direction YY. The second linear direction YY may be perpendicular to the first linear direction XX. Exemplarily, the direction of the rotation axis may be a vertical direction. The first linear direction XX and the second linear direction YY may be two horizontal directions perpendicular to each other. The eccentric member 3400 may be inserted into the long slot 3510. Furthermore, under the drive of the shaking drive assembly 3100, the eccentric member 3400 may rotate around the rotation axis, so that it may swing in the long slot 3510 along the second linear direction YY. When the eccentric member 3400 abuts against the slot wall of the long slot 3510 along the second linear direction YY, the eccentric member 3400 may push the connecting plate 3500 to swing along the first linear direction XX, thereby driving the shaking plate 3300 and the reaction vessel 1100 thereon to swing along the first linear direction XX. The connecting plate 3500 can be connected to the shaking plate 3300 by any suitable means such as welding, clamping or connecting with a connector.

[0113] For example, Figure 10-11As shown, the eccentric member 3400 may include a cam bearing follower. The cam bearing follower may include a mounting shaft 3410 and a roller 3420. The mounting shaft 3410 may be connected to the turntable 3200. The roller 3420 may be directly or indirectly connected to the shaking plate 3300. Specifically, one end of the mounting shaft 3410 may be connected to the turntable 3200. The roller 3420 may be connected to the other end of the mounting shaft 3410. The roller 3420 of the cam bearing follower may rotate relative to the mounting shaft 3410. In this way, the roller 3420 of the cam bearing follower may achieve rolling friction with the component connected thereto during rotation, thereby reducing the wear of the two and thereby increasing the service life. In an embodiment in which the shaking device 3000 includes a connecting plate 3500, the roller 3420 of the cam bearing follower may be inserted into the long slot 3510. In this way, the roller 3420 of the cam bearing follower may swing in the long slot 3510 along the second linear direction YY. In this way, when the roller 3420 of the cam bearing follower swings in the long groove 3510 along the second straight line direction YY, the roller 3420 of the cam bearing follower and the long groove 3510 can achieve rolling friction. In addition, the eccentric member 3400 can be a standard part, so there is no need to design it separately, and the design amount of the shaking device 3000 is reduced.

[0114] For example, Figure 10-11 As shown, the shaking device 3000 can also include a guide rail assembly 3600. The guide rail assembly 3600 can extend along the first straight line direction XX. The shaking plate 3300 can be connected to the guide rail assembly 3600. Under the drive of the shaking drive assembly 3100, the shaking plate 3300 and the reaction vessel 1100 can slide along the guide rail assembly 3600. By setting the guide rail assembly 3600, the shaking plate 3300 can be limited, so that the shaking plate 3300 can only be moved along the first straight line direction XX. In addition, the guide rail assembly 3600 can also improve the linearity and stability of the shaking plate 3300 moving along the first straight line direction XX.

[0115] For example, Figure 10-11 As shown, the guide rail assembly 3600 may include a first guide rail assembly 3601 and a second guide rail assembly 3602. Along the first straight direction XX, the first guide rail assembly 3601 and the second guide rail assembly 3602 may be located on both sides of the turntable 3200. The structures of the first guide rail assembly 3601 and the second guide rail assembly 3602 may be the same or different. The shaking plate 3300 may be connected to the first guide rail assembly 3601 and the second guide rail assembly 3602. By arranging the first guide rail assembly 3601 and the second guide rail assembly 3602, the limiting effect of the shaking plate 3300 is better. In addition, the first guide rail assembly 3601 and the second guide rail assembly 3602 may further improve the linearity and stability of the shaking plate 3300 moving along the first straight direction XX.

[0116] For example, Figure 10-11 As shown, at least one of the first guide rail assembly 3601 and the second guide rail assembly 3602 may include a connecting block 3610, a plurality of guide rails 3620 and a plurality of sliders 3630. Exemplarily, each of the first guide rail assembly 3601 and the second guide rail assembly 3602 may include a connecting block 3610, a plurality of guide rails 3620 and a plurality of sliders 3630. A plurality of guide rails 3620 may extend along the first straight line direction XX. And, a plurality of guide rails 3620 may be arranged at intervals along the second straight line direction YY. In the embodiment shown in the figure, the guide rail 3620 may include two. A plurality of sliders 3630 may correspond one to one and be slidably connected to a plurality of guide rails 3620. The connecting block 3610 may be connected between a plurality of sliders 3630 and the shaking plate 3300. By providing a plurality of guide rails 3620, the limiting effect on the shaking plate 3300, as well as the linearity and stability of the shaking plate 3300 moving along the first straight line direction XX, may be further improved.

[0117] For example, Figure 10-11 As shown, the shaking device 3000 can also include a mounting plate 3700. The guide rail assembly 3600 can be arranged on the mounting plate 3700. Specifically, a plurality of guide rails 3620 can be arranged on the mounting plate 3700. A through hole 3710 can be provided on the mounting plate 3700. The driving end of the shaking drive assembly 3100 can pass through the through hole 3710, so that it can be connected to the turntable 3200. Along the direction of the rotation axis, the mounting plate 3700 can isolate the main body of the shaking drive assembly 3100 and the shaking plate 3300 and the reaction vessel 1100 thereon, so that the liquid in the reaction vessel 1100 can be prevented from falling on the main body of the shaking drive assembly 3100.

[0118] For example, Figure 10-11As shown, the shake drive assembly 3100 may include a motor 3110 and a reducer 3120. The reducer 3120 may be connected between the motor 3110 and the turntable 3200. Specifically, the drive shaft of the motor 3110 may be connected to the input end of the reducer 3120. The output end of the reducer 3120 (i.e., the drive end of the shake drive assembly 3100) may be connected to the turntable 3200. In this way, under the drive of the motor 3110, the reducer 3120 may drive the turntable 3200 to rotate around the axis of rotation. The motor 3110 may adopt various types of motors known in the art or that may appear in the future, including but not limited to a servo motor or a stepper motor. The motor 3110 may adopt an explosion-proof motor. In this way, the structure of the shake drive assembly 3100 is simple and the manufacturing cost is low. Furthermore, by providing the reducer 3120, the rotation speed of the turntable 3200 can be adjusted, thereby adjusting the frequency of the shaking plate 3300 and the reaction container 1100 thereon shaking along the first straight line direction XX, and further adjusting the shaking effect. Furthermore, by providing the reducer 3120, the output torque can also be increased, so that a heavier reaction container 1100 can be placed on the shaking plate 3300.

[0119] For example, Figure 6 As shown, each group of reaction container assemblies 1200 may correspond to one shaking device 3000. Thus, the shaking device 3000 may drive all the reaction containers 1100 in the corresponding reaction container assembly 1200 to shake.

[0120] For example, Figure 6-7 As shown in Figure 12, the bubbling device 4000 may include a gas source triplet (not shown), a stop valve (not shown) and a speed regulating valve 4100. Along the flow direction of the gas bubbling device 4000, the gas source triplet, the stop valve, the speed regulating valve 4100 and the reaction vessel 1100 may be connected in sequence. The gas source triplet may be used to be connected to the first gas source. Nitrogen may pass through the gas source triplet, the stop valve and the speed regulating valve 4100 in sequence, so as to enter the reaction vessel 1100. The gas source triplet may purify, filter and reduce the pressure of the nitrogen passing through to the desired pressure. The stop valve may be used to control the on and off of nitrogen. The speed regulating valve 4100 may be used to adjust the flow of nitrogen, so that the reaction speed in the reaction vessel 1100 may be controlled by controlling the air intake in the reaction vessel 1100. The speed regulating valve 4100 includes but is not limited to an electromagnetic speed regulating valve or a manual speed regulating valve.

[0121] For example, Figure 6-7 As shown in FIG. 12 , the speed regulating valve 4100 may include a manual speed regulating valve, which has a simple structure and low cost. The manual speed regulating valve may be located at the front side of the polypeptide synthesis device to facilitate relevant personnel to adjust the manual speed regulating valve.

[0122] For example, Figure 6-7 As shown in Figure 12, the bubbling device 4000 can be arranged in a one-to-one correspondence with the reaction vessel assembly 1200. That is, each group of reaction vessel assembly 1200 can correspond to a bubbling device 4000. The bubbling device 4000 can also include a diverter 4200. The diverter 4200 can be provided with an air inlet 4210 and a plurality of air outlets 4220. The air outlets 4220 can be arranged in a one-to-one correspondence with all the reaction vessels 1100 of the corresponding reaction vessel assembly 1200. The air inlet 4210 can pass through the diverter 4200. One end of the plurality of air outlets 4220 can be connected to the middle between the two ends of the air inlet 4210. One end of the stop valve can be connected to the gas source triplet. The other end of the stop valve can be connected to the two ends of the air inlet 4210 respectively. The speed regulating valve 4100 can be arranged in a one-to-one correspondence with the air outlet 4220. The speed regulating valve 4100 can be connected between the other end of the plurality of air outlets 4220 and the reaction vessel 1100 in a one-to-one correspondence. In this way, after the nitrogen passes through the stop valve, it can enter the diverter 4200 from both ends of the air inlet 4210, and then flow out through the plurality of air outlets 4220. The nitrogen passing through the air outlets 4220 can pass through the corresponding speed regulating valve 4100, so as to enter the reaction vessel 1100. In this way, each speed regulating valve 4100 can individually adjust the flow rate on the gas path where it is located, so that the intake amount of different reaction vessels 1100 can be the same or different to meet different needs. In addition, since the nitrogen enters through the two ends of the air inlet 4210 and then flows out through the plurality of air outlets 4220, the nitrogen can flow to each air outlet 4220 relatively evenly, thereby avoiding the problem of too little nitrogen passing through the downstream air outlet 4220.

[0123] For example, Figure 6-7 As shown in Figure 12, the bubbling device 4000 may also include a three-way joint 4310 and a pair of shunt pipelines 4320. One end of the three-way joint 4310 may be connected to the stop valve. The other two ends of the three-way joint 4310 may be connected to the two ends of the air inlet 4210 through a pair of shunt pipelines 4320 in a one-to-one correspondence. The bubbling device 4000 may also include a branch pipeline 4330. The branch pipeline 4330 may be connected between the air outlet 4220 and the reaction vessel 1100 in a one-to-one correspondence. The speed regulating valve 4100 may be arranged on its corresponding branch pipeline 4330.

[0124] For example, Figure 6 and 12As shown, the peptide synthesis device may further include a heating device 5000. The heating device 5000 may be used to heat the reaction vessel 1100. The heating device 5000 may be disposed on the shaking device 3000. The shaking device 3000 may be used to drive the reaction vessel 1100 and the heating device 5000 to swing synchronously. The heating device 5000 may include any suitable heating device such as an electromagnetic heating device or an electric heating wire heating device. By providing the heating device 5000, the temperature in the reaction vessel 1100 may be within the optimal temperature threshold required for the reaction (e.g., 40-60 degrees), which may improve the speed and effect of the reaction.

[0125] For example, Figure 6 and 12 As shown, the heating device 5000 may include a support plate 5100 and a heating element 5200. The support plate 5100 may be disposed on the shaking device 3000. The heating element 5200 may be disposed on the support plate 5100 by any suitable means such as welding, bonding or screw connection, so that it can be fixed to the support plate 5100. A placement hole 5210 and a heating circulation channel 5220 may be provided on the heating element 5200. The reaction vessel 1100 may be detachably inserted into the placement hole 5210. Specifically, the reaction cup 1110 may be detachably inserted into the placement hole 5210. The heating circulation channel 5220 may surround the placement hole 5210. The heating circulation channel 5220 may have an inlet 5221 and an outlet 5222. For example, liquids such as hot water or hot oil may enter the heating circulation channel 5220 through the inlet 5221, and then flow around the placement hole 5210 and then flow out through the outlet 5222. Combined with Figure 1As shown, the inlet 5221 can be connected to the thermostat 9200. The outlet 5222 can be connected to the thermostat 9200. Therefore, the peptide synthesis device can also include the thermostat 9200. Exemplarily, the inlet 5221 and the outlet 5222 can be located at the rear side of the heating element 5200 to make more space for the front side. Exemplarily, along the extension direction of the heating circulation channel 5220, the heating circulation channel 5220 can be generally rectangular, circular or spiral, etc., as long as it can surround the placement hole 5210. In some embodiments, the heating circulation channel 5220 may include a first heating hole 5223, a second heating hole 5224, a third heating hole 5225 and a fourth heating hole 5226 arranged on the heating element 5200. The first heating hole 5223 and the second heating hole 5224 can extend in the front-to-back direction, respectively, and be located on the left and right sides of the heating element 5200. The third heating hole 5225 and the fourth heating hole 5226 can extend in the left and right directions, respectively, and are located at the front and rear sides of the heating element 5200. The end of the first heating hole 5223 can be configured as an inlet 5221. The end of the second heating hole 5224 can be configured as an outlet 5222. The ends of the third heating hole 5225 and the fourth heating hole 5226 can be blocked with sealing plugs, respectively. The third heating hole 5225 and the fourth heating hole 5226 can be connected to the first heating hole 5223 and the second heating hole 5224 on the left and right sides of the heating element 5200, respectively. The first heating hole 5223, the second heating hole 5224, the third heating hole 5225 and the fourth heating hole 5226 can surround the placement hole 5210. In this way, after the liquid enters the first heating hole 5223 through the inlet 5221, it can flow to the third heating hole 5225 and the fourth heating hole 5226, respectively, and then after merging in the second heating hole 5224, it flows out through the outlet 5222. Exemplarily, the first heating hole 5223, the second heating hole 5224, the third heating hole 5225, and the fourth heating hole 5226 may be located on the same horizontal plane. The first heating hole 5223, the second heating hole 5224, the third heating hole 5225, and the fourth heating hole 5226 may have the same aperture.

[0126] The supporting member 1120 may be arranged on the supporting plate 5100 by any suitable means such as plugging, welding, bonding or screw connection. In addition, the feeding tube 1130 may be located below the placement hole 5210. When the reaction cup 1110 is inserted into the placement hole 5210, it may be connected to the supporting member 1120 and communicate with the feeding tube 1130.

[0127] In the heating device 5000 provided in the embodiment of the present invention, the liquid in the heating circulation channel 5220 can exchange heat with the reaction vessel 1100 through the heating element 5200, so that the reaction vessel 1100 can be heated. Since the heating circulation channel 5220 surrounds the placement hole 5210, it can heat the reaction vessel 1100 from all sides, and the heating rate is relatively high. In addition, since the liquid in the heating circulation channel 5220 is flowing, the liquid with a lower temperature after heat exchange can flow out, and the liquid with a high temperature can flow in, so the heating circulation channel 5220 can keep the reaction vessel 1100 at a constant temperature. In addition, the heating device 5000 can meet explosion-proof requirements.

[0128] For example, Figure 1 As shown, the thermostat 9200 can start a water heating cycle. When the water temperature reaches the set temperature, the hot water can flow out of the thermostat 9200, pass through the heating circulation channel 5220, and then flow back into the thermostat 9200 to be heated again, thereby completing a constant temperature cycle.

[0129] The heating element 5200 can be made of any suitable material such as plastic, copper or iron. For example, the heating element 5200 can be made of aluminum. Aluminum is a metal with good thermal conductivity, and its thermal conductivity is about 237W / m·K (watts per meter per Kelvin), which makes it ideal as a heat conduction material. In addition, aluminum is light in weight and low in cost. In addition, the high reflectivity of aluminum also helps to improve its efficiency in heat exchange.

[0130] For example, Fig.15 As shown, the heating element 5200 may also be provided with a sensor hole 5240. The sensor hole 5240 may be connected to the heating circulation channel 5220. The heating device 5000 may also include a sensor connected to the sensor hole 5240. The sensor may be used to detect the temperature of the liquid in the heating circulation channel 5220, so that it can cooperate with the controller and the above-mentioned thermostat 9200 and other components to achieve the purpose of accurately controlling the temperature of the liquid.

[0131] For example, Figure 13-14 As shown, the front side of the heating element 5200 may be provided with an observation hole 5230. The observation hole 5230 may be communicated with the placement hole 5210. The observation hole 5230 may be circular, rectangular or any other suitable shape. In the embodiment shown in the figure, the observation hole 5230 may be a waist hole extending along the extension direction of the placement hole 5210. The sight of the relevant personnel can pass through the observation hole 5230, so that the state (such as reaction process and / or reaction speed) of the reaction vessel 1100 in the placement hole 5210 can be observed.

[0132] For example, Figure 13-14As shown, the observation hole 5230 can be located below the heating circulation channel 5220. Since the reactants are usually deposited at the bottom of the reaction vessel 1100, by setting the observation hole 5230 below the heating circulation channel 5220, relevant personnel can observe the state of the bottom of the reaction vessel 1100. In addition, since the observation hole 5230 will cause the structural strength of the heating element 5200 to decrease, by placing the observation hole 5230 close to the support plate 5100, the support plate 5100 can suppress the influence of the observation hole 5230 on the decrease in the structural strength of the heating element 5200.

[0133] For example, Figure 6 and 13 -14, 6-7 and 12, the heating device 5000 can be arranged in a one-to-one correspondence with the reaction vessel assembly 1200. That is, each group of reaction vessel assemblies 1200 can correspond to a heating device 5000. A plurality of placement holes 5210, such as two, three or more, can be arranged at intervals on the heating element 5200. In the embodiment shown in the figure, the number of placement holes 5210 can be ten. A plurality of placement holes 5210 can be arranged along a straight line, an arc or any other suitable manner. The structure of each placement hole 5210 can be the same or different. The heating circulation channel 5220 can surround the plurality of placement holes 5210. The plurality of placement holes 5210 can be used for all the reaction vessels 1100 of the corresponding reaction vessel assembly 1200 to be inserted one by one. The heating circulation channel 5220 can heat a plurality of placement holes 5210 at the same time, and the structure is relatively simple and convenient for processing and manufacturing.

[0134] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by directional words such as "front", "back", "up", "down", "left", "right", "lateral", "vertical", "vertical", "horizontal", "top", "bottom", etc. is usually based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the scope of protection of the present invention; the directional words "inside" and "outside" refer to the inside and outside relative to the contour of each component itself.

[0135] For ease of description, regional relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the regional positional relationship between one or more components or features shown in the figure and other components or features. It should be understood that regional relative terms include not only the orientation of the components as described in the figure, but also different orientations in use or operation. For example, if the components in the accompanying drawings are inverted as a whole, the components "above other components or features" or "above other components or features" will include the situation where the components are "below other components or structures" or "below other components or structures". Therefore, the exemplary term "above" may include both "above" and "below". In addition, these components or features may also be positioned at other different angles (e.g., rotated 90 degrees or other angles), and this article is intended to include all of these situations.

[0136] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, parts, components and / or combinations thereof.

[0137] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the numbers used in this way can be interchanged where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.

[0138] The present invention has been described by the above embodiments, but it should be understood that the above embodiments are only for the purpose of example and description, and are not intended to limit the present invention to the scope of the described embodiments. In addition, it can be understood by those skilled in the art that the present invention is not limited to the above embodiments, and more variations and modifications can be made according to the teachings of the present invention, and these variations and modifications all fall within the scope of the protection claimed by the present invention. The protection scope of the present invention is defined by the attached claims and their equivalents.

Claims

1. A polypeptide synthesis device, characterized in that: include: A reaction vessel device, wherein the reaction vessel device has a reaction vessel, and the reaction vessel is used for carrying out a reaction; A liquid adding device, the liquid adding device is used to add liquid into the reaction container; A shaking device, on which the reaction container is arranged, and the shaking device is used to drive the reaction container to shake; as well as A bubbling device is connected to the reaction container and is used to bubble gas into the reaction container.

2. The peptide synthesis device according to claim 1, characterized in that: The shaking device includes a shaking drive assembly, a turntable, a shaking plate and an eccentric member, wherein the driving end of the shaking drive assembly is connected to the turntable to drive the turntable to rotate around a rotation axis, the reaction container is arranged on the shaking plate, the eccentric member is connected between the turntable and the shaking plate, the eccentric member is not coaxial with the rotation axis, and the eccentric member drives the shaking plate and the reaction container to swing along a first straight line direction perpendicular to the rotation axis when driven by the shaking drive assembly.

3. The polypeptide synthesis device according to claim 2, characterized in that: The shaking device also includes a connecting plate, on which is provided a long slot extending along a second straight line direction perpendicular to the rotation axis and the first straight line direction. The eccentric piece is inserted into the long slot and can swing in the long slot under the drive of the shaking drive assembly. The connecting plate is connected to the shaking plate.

4. The peptide synthesis device according to claim 1, characterized in that: The bubbling device comprises a gas source triplet, a stop valve and a speed regulating valve. Along the flow direction of the gas bubbled into the bubbling device, the gas source triplet, the stop valve, the speed regulating valve and the reaction container are connected in sequence.

5. The polypeptide synthesis device according to claim 4, characterized in that: The reaction container device includes multiple groups of reaction container assemblies, each group of the reaction container assemblies includes multiple reaction containers, the bubbling device is arranged in a one-to-one correspondence with the multiple groups of reaction container assemblies, the bubbling device also includes a diverter, the diverter is provided with an air inlet and multiple air outlets, the multiple air outlets are arranged in a one-to-one correspondence with all the reaction containers of the reaction container assembly corresponding to them, the air inlet runs through the diverter, one end of the shut-off valve is connected to the gas source triplet, and the other end is connected to the two ends of the air inlet, one end of the multiple air outlets is respectively connected to the middle part between the two ends of the air inlet, and the speed regulating valve is connected one-to-one between the other end of the multiple air outlets and the reaction container.

6. The peptide synthesis device according to claim 1, characterized in that: The peptide synthesis equipment also includes a heating device for heating the reaction container, wherein the heating device is arranged on the shaking device, and the shaking device is used to drive the reaction container and the heating device to shake synchronously.

7. The peptide synthesis device according to claim 6, characterized in that: The heating device includes a support plate and a heating element, wherein the support plate is arranged on the shaking device, and the heating element is arranged on the support plate. The heating element is provided with a placement hole and a heating circulation channel surrounding the placement hole, and the reaction container can be detachably inserted into the placement hole.

8. The peptide synthesis device according to claim 7, characterized in that: The reaction container device includes multiple groups of reaction container assemblies, each group of the reaction container assemblies includes multiple reaction containers, the heating device is arranged in a one-to-one correspondence with the multiple groups of bubbling devices, and a plurality of placement holes are arranged at intervals on the heating element. The plurality of placement holes are used for one-to-one insertion of all the reaction containers of the corresponding reaction container assemblies, and the heating circulation channel surrounds the plurality of placement holes.

9. The peptide synthesis device according to claim 1, characterized in that: The top of the reaction container is provided with a top opening and the bottom is provided with a drainage port. The polypeptide synthesis equipment also includes a drainage device, which includes a positive pressure drainage component and a negative pressure drainage component. The positive pressure drainage component is used to inject gas into the reaction container from the top opening to apply pressure to the reaction container, and the negative pressure drainage component is connected to the drainage port to extract the liquid in the reaction container through the drainage port.

10. The polypeptide synthesis device according to claim 9, characterized in that: The drainage device also includes a drainage pipeline and a clamping tube assembly. The drainage pipeline is connected between the negative pressure drainage assembly and the drainage port. The clamping tube assembly is used to clamp the drainage pipeline to cut off the drainage pipeline.

11. The peptide synthesis device according to claim 10, characterized in that: The reaction container device includes multiple groups of reaction container assemblies, each group of reaction container assemblies includes multiple reaction containers, the drainage pipeline includes multiple shunt drainage pipelines, the multiple shunt drainage pipelines are arranged in a one-to-one correspondence with all the reaction containers of the corresponding reaction container assemblies, the shunt drainage pipelines are connected between the negative pressure drainage assembly and the corresponding drainage port, and the tube clamping assembly is used to clamp the multiple shunt drainage pipelines to cut off the multiple shunt drainage pipelines.

12. The peptide synthesis device according to claim 9, characterized in that: The reaction container device includes multiple groups of reaction container assemblies, each group of the reaction container assemblies includes multiple reaction containers, the positive pressure drainage assembly is arranged in a one-to-one correspondence with all the reaction containers of the corresponding reaction container assembly, the positive pressure drainage assembly includes a three-way joint and a connecting pipe, the first end of the three-way joint is one-to-one correspondingly toward each top opening of the corresponding reaction container assembly, along the arrangement direction of the three-way joints, the second end of the first three-way joint is used to connect to the gas source, the third end of the last three-way joint is sealed with a plug, and the connecting pipe is connected between the second end and the third end of two adjacent three-way joints.

13. The peptide synthesis device according to claim 1, characterized in that: The liquid adding device includes a plurality of liquid injection parts, a liquid outlet part and a plurality of switching valves corresponding to the plurality of liquid injection parts one by one. The switching valve is arranged between the corresponding liquid injection part and the plurality of liquid outlet parts. The switching valve has a liquid suction state that connects the corresponding liquid injection part with the liquid storage container and a liquid injection state that connects the corresponding liquid injection part with the liquid outlet part. The liquid injection part is used to suck the liquid in the liquid storage container into the liquid injection part when the corresponding switching valve is in the liquid suction state, and is used to inject the liquid in the liquid injection part into the reaction container below the liquid outlet part through the liquid outlet part when the corresponding switching valve is in the liquid injection state.

14. The peptide synthesis device according to claim 1, characterized in that: The reaction container includes a reaction cup, a supporting member and a feeding tube. The reaction cup is used for conducting a reaction. A bottom opening is arranged at the bottom of the reaction cup. The reaction cup is supported on the supporting member, and the supporting member is arranged on the shaking device. The feeding tube extends downward from the supporting member. A liquid inlet and an air inlet connected to the bottom opening are arranged on the side of the feeding tube. The liquid adding device is connected to the liquid inlet for adding liquid into the reaction cup. The bubbling device is connected to the air inlet for bubbling gas into the reaction cup.

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