Pepper oleoresin as well as preparation method and application thereof

Abstract

The invention relates to the technical field of plant extraction, and particularly discloses pepper oleoresin as well as a preparation method and application thereof. The refining method of the pepper oleoresin comprises the following steps: mixing a pepper oleoresin raw material with an alkaline alcohol solution, carrying out heat preservation, standing and layering at 40-45 DEG C, and respectively concentrating to obtain refined pepper oleoresin and a crude piperine extract; the alcohol concentration of the alkaline alcohol solution is 70-75 degrees, and the pH value of a system after the pepper oleoresin raw material and the alkaline alcohol solution are mixed is 8-9; the mass volume ratio of the pepper oleoresin raw material to the alkaline alcohol solution is 1: (1.5-2) g / mL. The pepper oleoresin obtained by the refining method disclosed by the invention is high in glucosylceramide content, uniform and stable in product and not easy to crystallize and layer. According to the refining method, the piperine product can be obtained at the same time, and comprehensive utilization of raw materials is achieved. The method is simple in technological process, low in cost and easy to realize continuous industrial production.

Description

Technical Field

[0001] The invention relates to the technical field of plant extraction, in particular to pepper oleoresin and a preparation method and application thereof. Background Art

[0002] Pepper (especially black pepper) is rich in volatile oils and flavor components such as piperine, and is currently a widely used spice. Pepper oleoresin is an effective ingredient extracted from pepper. It has a unique flavor and is easy to use. It is an important food additive and is deeply loved by people. It is mainly used in meat, canned food, soups, sauces and beverages. At the same time, pepper oleoresin also has certain preservation and antiseptic effects, and is the development trend of pepper product application development.

[0003] Currently, the conventional low-content pepper oleoresin on the market is made from high-content pepper oleoresin, which is diluted by adding pepper essential oil and emulsifier. During the storage process, the product will crystallize, affecting the use of the product. The crystallization problem needs to be effectively solved. Summary of the invention

[0004] One of the purposes of the present invention is to provide a preparation method and application of pepper oleoresin which is not easy to crystallize and delaminate.

[0005] The invention provides a method for refining pepper oleoresin, which comprises: mixing a pepper oleoresin raw material with an alkaline alcohol solution, keeping the mixture at 40-45°C for standing and stratification, and respectively concentrating the mixture to obtain refined pepper oleoresin and piperine crude extract; the alcohol concentration of the alkaline alcohol solution is 70-75°, and the pH value of the system after the pepper oleoresin raw material and the alkaline alcohol solution are mixed is 8-9; and the mass volume ratio of the pepper oleoresin raw material to the alkaline alcohol solution is 1:(1.5-2) g / mL.

[0006] The invention aims at the problem that pepper oleoresin in the prior art is prone to crystallization and stratification when used, and repeatedly studies are conducted. It is finally found that after the pepper oleoresin raw material is separated at a specific temperature using alkaline alcohol with a specific concentration and pH, the refined pepper oleoresin in the upper layer is not easy to crystallize and stratify when used, thereby improving the product quality. At the same time, after stratification, a crude piperine extract can be obtained in the lower layer, and a high-content piperine product can be obtained after further refining, thereby realizing the comprehensive utilization of the raw materials.

[0007] Specifically, under specific conditions, the present invention removes free fatty acids in the raw materials by alkaline alcohol, and the generated fatty acid salts (soapstock) can carry away impurities such as glucosylceramide and chlorophyll, and under specific conditions, part of piperine can be enriched by alkaline alcohol to inhibit the production of piperine crystals.

[0008] The present invention also found that when the pepper oleoresin raw material is dissolved in alcohol, the alcohol degree is too high or the amount of alcohol added is too large, the ability to dissolve pepper oleoresin is stronger, and the separation of pepper oleoresin and piperine crude extract cannot be effectively achieved; the alcohol degree is too low or the amount of alcohol added is too low, the crystallization problem of the upper pepper oleoresin cannot be solved, and the piperine content in the lower layer piperine crude extract is low. Even if further refined, the ideal piperine content cannot be obtained. In addition, when the pH is too low during refining, the content and / or yield of the lower layer piperine crude extract will be reduced, and the crystallization phenomenon of the upper layer pepper oleoresin cannot be solved; when the pH is too high, the upper layer pepper oleoresin needs to be washed with pure water to reduce the pH before application, which will produce a large amount of wastewater, which is not conducive to industrial production. The stratification temperature is too low, there are more impurities in the piperine crude extract, and the product content is low; the stratification temperature is too high, the pepper oleoresin has a strong ability to dissolve in alkaline alcohol, and effective stratification cannot be achieved. When the alcohol degree, material-liquid ratio, pH value and stratification temperature in the scheme of the present invention are specifically matched, high-quality pepper oleoresin that is not easy to crystallize and stratify is obtained, and the obtained piperine crude extract has a high piperine content and a high yield, and can be used for further refining to prepare a high-purity product.

[0009] The raw material of the pepper oleoresin of the present invention is a product known in the art, and its main components are 55-60% of piperine, 2-3% of volatile oil, 3-5% of fatty acid, 1.5-1.8% of glycerol glycolipid, more than 3% of piperine, less than 4% of glucosylceramide, and more than 12% of other amides. The raw material of the pepper oleoresin can be obtained commercially or prepared according to a method known in the art. For example, the preparation method can be as follows: the pepper raw material is tableted and then steam distilled, and the distilled pepper is extracted using a mixed solvent of n-hexane and ethyl acetate (the volume ratio of the two is 7:3), and the pepper oleoresin is obtained after concentration and deodorization.

[0010] When the pepper oleoresin raw material is mixed with the alkaline alcohol solution in the present invention, the alkaline alcohol solution can be prepared first and then mixed with the pepper oleoresin raw material, or the pepper oleoresin raw material and the alcohol solution can be mixed first and then the pH is adjusted, as long as the pH value of the mixed system can be guaranteed to be 8-9.

[0011] When the refined pepper oleoresin and piperine crude extract are concentrated, the concentration method used is a method known in the art, such as: the concentration temperature is 60-70° C., and the vacuum degree is -0.09 to -0.085 MPa.

[0012] In the purification method of the present invention, the alcohol in the alkaline alcohol solution is ethanol.

[0013] The purification method of the present invention further comprises a step of crystallizing the crude piperine extract.

[0014] In the refining method of the present invention, the crystallization method is to dissolve the crude piperine extract in ethanol (preferably the mass volume ratio of the crude piperine extract to ethanol is 1:2 g / mL, and the solution is dissolved at 60°C), and then cool the mixture to 0°C at a rate of 8-12°C / h (preferably 10°C / h) and keep the mixture warm for 1.5-2.5h under a stirring speed of 25-35r / min (preferably 30r / min).

[0015] The crude piperine extract obtained by the refining method of the present invention has a piperine content of more than 95% and a content yield of more than 85% after refining.

[0016] The invention also provides a pepper oleoresin, which is prepared by the above refining method.

[0017] The content of piperine in the pepper oleoresin of the invention is between 25% and 35%, the content of piperine is less than 3%, and the content of glucosylceramide is more than 4%.

[0018] The pepper oleoresin refined by the method of the invention has high glucosylceramide content and is not easy to crystallize or delaminate.

[0019] The invention also provides a formulated pepper oleoresin, which comprises the pepper oleoresin.

[0020] Those skilled in the art can conventionally prepare the above-mentioned pepper oleoresin according to needs to obtain products with different piperine contents.

[0021] The prepared pepper oleoresin of the present invention also comprises pepper essential oil and emulsifier.

[0022] In the prepared pepper oleoresin of the present invention, the emulsifier is Tween 80 and / or propylene glycol.

[0023] After the pepper oleoresin refined by the conventional method is formulated, the obtained product has good stability, and no crystals or stratification occurs after centrifugation at 3000 r / min for 15 minutes. Compared with the low-content pepper oleoresin directly diluted with the conventional high-content pepper oleoresin, the glucosylceramide content is high, the spiciness is quick to take on, and the intensity is high.

[0024] The invention also provides application of the refining method in preventing crystallization and stratification of pepper oleoresin.

[0025] The beneficial effects of the present invention are at least: The pepper oleoresin obtained by the refining method of the present invention has a high content of glucosylceramide, and the product is uniform and stable and not easy to crystallize and stratify. In addition, the refining method of the present invention can simultaneously obtain the piperine product, thereby realizing the comprehensive utilization of raw materials. In addition, the process flow of the method of the present invention is simple, the cost is low, and continuous industrial production is easy to realize. DETAILED DESCRIPTION

[0026] The preferred embodiments of the present invention will be described in detail below in conjunction with examples. It should be understood that the following examples are provided only for the purpose of illustration and are not intended to limit the scope of the present invention. Those skilled in the art may make various modifications and substitutions to the present invention without departing from the purpose and spirit of the present invention.

[0027] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples are commercially available or prepared according to conventional methods in the art unless otherwise specified.

[0028] In the present invention, the detection method of piperine and piperine is high performance liquid chromatography (HPLC), and the detection method of glucosylceramide is high performance liquid chromatography-quadrupole time of flight tandem mass spectrometry (HPLC-Q-TOF-MS). Specifically, the sample is pretreated before detection. The pretreatment method is: weigh 0.1-0.2g of sample in a 100mL brown volumetric flask, dilute with methanol, transfer 5mL of diluted liquid into a 100mL volumetric flask, dilute with methanol, extract the diluted liquid with a syringe, pass through a filter membrane into a liquid phase vial for subsequent analysis and detection.

[0029] Specifically: 1. Detection method of piperine and piperine: A Luna C18 column was used with methanol:water (77:23) as the mobile phase, a flow rate of 1.0 mL / min, a detection wavelength of 343 nm, and a column temperature of 35°C.

[0030] 2. Glucosylceramide detection method: ① Liquid phase (liquid phase model: Waters) conditions: chromatographic column: WatersBEH C18 (2.1×150 mm, 1.7 μm); column temperature: 40 ℃; mobile phase: A-0.1% formic acid water; B-isopropanol / acetonitrile = 1:1; gradient elution conditions are as shown in Table 1.

[0031] Table 1 ②Mass spectrometry (mass spectrometer model: Waters time-of-flight mass spectrometer) conditions: ion source: ESI; mode: positive ion, sensitivity; scanning molecular weight range: 50-2000 Da; collision gas: argon; collision energy: 20-40 V; capillary voltage: (+) 3 kV; cone voltage: 40 V; source temperature: 120 ℃; desolvation temperature: 300 ℃; cone gas flow rate: 50 L / h; desolvation gas flow rate: 700 L / min.

[0032] Example 1 This embodiment provides a method for refining pepper oleoresin, which is as follows: S1, ethanol dissolution: Take 100g of pepper oleoresin raw material (whose main components are 56.13% piperine, 2.59% volatile oil, 3.28% fatty acid, 1.64% glycerol glycolipid, 3.38% glucosylceramide, 3.73% piperine), add 200mL 75% ethanol to dissolve; S2, alkaline alcohol separation: adjusting the pH value of the ethanol-containing pepper oleoresin obtained in step S1 to 9 using sodium hydroxide; S3, separation: the alkaline alcohol pepper oleoresin solution obtained in step S2 is stirred at 45°C, kept warm and allowed to stand for stratification, and concentrated at a concentration temperature of 65°C and a vacuum degree of -0.085MPa to obtain 50.25g of refined pepper oleoresin in the upper layer and 49.75g of crude piperine extract in the lower layer; The refined pepper oleoresin contains 31.85% piperine, 2.69% piperine and 4.26% glucosylceramide. S4. Product preparation / preparation: 8 g of pepper essential oil and 100 g of propylene glycol were added to the refined pepper oleoresin on the upper layer to prepare a prepared pepper oleoresin having a piperine content of 10.11%, a piperine content of 0.85%, and a glucosylceramide content of 1.35%.

[0033] The crude piperine extract in the lower layer was dissolved in twice the volume of ethanol (the mass volume ratio of crude piperine extract to ethanol was 1:2 g / mL) at 60°C, and the temperature was lowered to 0°C at a rate of 10°C / h while stirring at 30 r / min. The temperature was kept for 2 h to obtain 36.01 g of piperine with a content of 96.30%. The content yield of the cooling crystallization step was 86.42%.

[0034] Product evaluation: 8 g of the refined pepper oleoresin in the upper layer of step S3 and the prepared pepper oleoresin in step S4 were respectively placed in 10 mL centrifuge tubes and centrifuged at 3000 r / min for 15 min. No crystals or stratification occurred.

[0035] A control group with the same piperine content as the prepared pepper oleoresin was set up. The preparation method was as follows: 11.49g of pepper oleoresin raw material was directly mixed with 3.23g of pepper essential oil and 49.1g of propylene glycol to obtain a resin as the control group, and the piperine content was 10.11%, the piperine content was 0.67%, and the glucosylceramide content was 0.61%. Under the same centrifugal conditions, 3.18g of crystals appeared in the control group, and stratification occurred. The piperine content in the upper layer of pepper oleoresin was 24.59%.

[0036] In addition, the pepper oleoresin prepared with refined pepper oleoresin in this embodiment is more spicy and has a higher intensity (high glucosylceramide content) than the low-content pepper oleoresin (control group) directly diluted with high-content pepper oleoresin.

[0037] Example 2 This embodiment provides a method for refining pepper oleoresin, which is as follows: S1, ethanol dissolution: Take 100g of pepper oleoresin raw material (whose main components are 56.13% piperine, 2.13% volatile oil, 3.69% fatty acid, 1.53% glycerol glycolipid, 3.25% glucosylceramide, 3.88% piperine), add 200mL 75% ethanol to dissolve; S2, alkaline alcohol separation: adjusting the pH value of the ethanol-containing pepper oleoresin obtained in step S1 to 8 using sodium hydroxide; S3, separation: the alkaline alcohol pepper oleoresin solution obtained in step S2 is stirred at 40°C, kept warm and allowed to stand for stratification, and concentrated at a concentration temperature of 65°C and a vacuum degree of -0.085MPa to obtain 48.56g of refined pepper oleoresin in the upper layer and 51.44g of crude piperine extract in the lower layer; The refined pepper oleoresin contains 28.87% piperine, 2.63% piperine and 4.71% glucosylceramide. S4. Product preparation / preparation: 5 g of pepper essential oil and 50 g of Tween 80 were added to the refined pepper oleoresin on the upper layer to prepare a prepared pepper oleoresin having a piperine content of 13.54%, a piperine content of 1.23%, and a glucosylceramide content of 2.21%.

[0038] The crude piperine extract in the lower layer was dissolved in twice the volume of ethanol at 60°C, cooled to 0°C at a rate of 10°C / h under a stirring speed of 30 r / min, and kept warm for 2 h to obtain 37.73 g of piperine with a content of 97.17%, and the content yield of the cooling crystallization step was 87.06%.

[0039] Product evaluation: 8 g of the refined pepper oleoresin in the upper layer of step S3 and the prepared pepper oleoresin in step S4 were respectively placed in 10 mL centrifuge tubes and centrifuged at 3000 r / min for 15 min. No crystals or stratification occurred.

[0040] Another resin obtained by mixing 16.02g of pepper oleoresin raw material with 3.21g of pepper essential oil and 47.2g of Tween 80 was set as a control group, and its piperine content was 13.54%, piperine content was 0.94%, and glucosylceramide content was 0.78%. Under the same centrifugal conditions, 3.36g of crystals appeared in the control group, and stratification occurred. The piperine content in the upper layer of pepper oleoresin was 21.99%.

[0041] In addition, the pepper oleoresin prepared with refined pepper oleoresin in this embodiment is more spicy and has a higher intensity (high glucosylceramide content) than the low-content pepper oleoresin (control group) directly diluted with high-content pepper oleoresin.

[0042] Example 3 This embodiment provides a method for refining pepper oleoresin, which is as follows: S1, ethanol dissolution: take 100g pepper oleoresin raw material (same as Example 2), add 150mL 70% ethanol to dissolve; S2, alkaline alcohol separation: adjusting the pH value of the ethanol-containing pepper oleoresin obtained in step S1 to 8 using sodium hydroxide; S3, separation: the alkaline alcohol pepper oleoresin solution obtained in step S2 is stirred at 40°C, kept warm and allowed to stand for stratification, and concentrated at a concentration temperature of 65°C and a vacuum degree of -0.085MPa to obtain 47.36g of refined pepper oleoresin in the upper layer and 52.64g of crude piperine extract in the lower layer; The refined pepper oleoresin contains 29.32% piperine, 2.85% piperine, and 4.83% glucosylceramide. S4. Product preparation / preparation: 4 g of pepper essential oil and 30 g of propylene glycol were added to the refined pepper oleoresin in the upper layer to prepare a prepared pepper oleoresin having a piperine content of 17.07%, a piperine content of 1.66%, and a glucosylceramide content of 2.81%.

[0043] The crude piperine extract in the lower layer was dissolved in twice the volume of ethanol at 60°C, cooled to 0°C at a rate of 10°C / h under a stirring speed of 30 r / min, kept warm for 2 h, and crystallized to obtain 37.82 g of piperine with a content of 95.32%. The content yield of the cooling crystallization step was 85.33%.

[0044] Product evaluation: 8 g of the refined pepper oleoresin in the upper layer of step S3 and the prepared pepper oleoresin in step S4 were respectively placed in 10 mL centrifuge tubes and centrifuged at 3000 r / min for 15 min. No crystals or stratification occurred.

[0045] Another resin obtained by mixing 14.19g of pepper oleoresin raw material with 2.293g of pepper essential oil and 30.15g of propylene glycol was set as the control group, and its piperine content was 17.08%, piperine content was 1.18%, and glucosylceramide content was 0.99%. Under the same centrifugal conditions, 3.26g of crystals appeared in the control group, and stratification occurred. The piperine content in the upper layer of pepper oleoresin was 22.91%.

[0046] In addition, the pepper oleoresin prepared with refined pepper oleoresin in this embodiment is more spicy and has a higher intensity (high glucosylceramide content) than the low-content pepper oleoresin (control group) directly diluted with high-content pepper oleoresin.

[0047] Comparative Example 1 This comparative example provides a method for refining pepper oleoresin, which is as follows: S1, ethanol dissolution: take 100g pepper oleoresin raw material (same as in Example 1), add 200mL 75% ethanol to dissolve; S2, alkaline alcohol separation: adjusting the pH value of the ethanol-containing pepper oleoresin obtained in step S1 to 9 using sodium hydroxide; S3, separation: the alkaline alcohol pepper oleoresin solution obtained in step S2 is stirred at 35°C, kept warm and allowed to stand for stratification, and concentrated at a concentration temperature of 65°C and a vacuum degree of -0.085MPa to obtain 40.01g of refined pepper oleoresin in the upper layer and 59.99g of crude piperine extract in the lower layer; The refined pepper oleoresin contains 27.47% piperine, 3.85% piperine and 3.59% glucosylceramide.

[0048] The crude piperine extract in the lower layer was crystallized with ethanol according to the method of Example 1 to obtain 40.65 g of piperine with a content of 91.53%. The yield of the cooling crystallization step was 82.43%, and the piperine content did not reach 95%.

[0049] Product evaluation: Take 8g of the refined pepper oleoresin in a 10mL centrifuge tube and centrifuge at 3000r / min for 15min. Crystallization and stratification will occur.

[0050] Comparative Example 2 This comparative example provides a method for refining pepper oleoresin, which is as follows: S1, ethanol dissolution: take 100g pepper oleoresin raw material (same as in Example 1), add 200mL 75% ethanol to dissolve; S2, alkaline alcohol separation: adjusting the pH value of the ethanol-containing pepper oleoresin obtained in step S1 to 9 using sodium hydroxide; S3, separation: the basic alcohol pepper oleoresin solution obtained in step S2 is stirred at 48° C., kept warm and allowed to stand for stratification, and the pepper oleoresin is completely dissolved in the basic alcohol and cannot be separated.

[0051] Comparative Example 3 This comparative example provides a method for refining pepper oleoresin, which is as follows: S1, ethanol dissolution: take 100g pepper oleoresin raw material (same as in Example 1), add 200mL 65% ethanol to dissolve; S2, alkaline alcohol separation: adjusting the pH value of the ethanol-containing pepper oleoresin obtained in step S1 to 9 using sodium hydroxide; S3, separation: the alkaline alcohol pepper oleoresin solution obtained in step S2 was stirred at 45°C, kept warm and allowed to stand for stratification, and 42.60g of refined pepper oleoresin in the upper layer and 57.40g of crude piperine extract in the lower layer were obtained at a concentration temperature of 65°C and a vacuum degree of -0.085MPa; The refined pepper oleoresin contains 28.28% piperine, 2.58% piperine and 3.57% glucosylceramide.

[0052] The crude piperine extract in the lower layer was crystallized with ethanol according to the method of Example 1 to obtain 39.90 g of piperine with a content of 91.24%. The yield of the cooling crystallization step was 83.06%, and the piperine content did not reach 95%.

[0053] Product evaluation: Take 8g of the refined pepper oleoresin in a 10mL centrifuge tube and centrifuge at 3000r / min for 15min, and crystallization and stratification will occur.

[0054] Comparative Example 4 This comparative example provides a method for refining pepper oleoresin, which is as follows: S1, ethanol dissolution: take 100g pepper oleoresin raw material (same as in Example 1), add 100mL 70% ethanol to dissolve; S2, alkaline alcohol separation: adjusting the pH value of the ethanol-containing pepper oleoresin obtained in step S1 to 8 using sodium hydroxide; S3, separation: the alkaline alcohol pepper oleoresin solution obtained in step S2 was stirred at 40°C, kept warm and allowed to stand for stratification, and 43.13g of refined pepper oleoresin in the upper layer and 56.87g of crude piperine extract in the lower layer were obtained at a concentration temperature of 65°C and a vacuum degree of -0.085MPa; The refined pepper oleoresin contains 28.69% piperine, 2.91% piperine and 3.93% glucosylceramide.

[0055] The crude piperine extract in the lower layer was crystallized using ethanol according to the method of Example 1 to obtain 39.80 g of piperine with a content of 92.19%. The yield of the cooling crystallization step was 84.01%, and the piperine content did not reach 95%.

[0056] Product evaluation: Take 8g of the refined pepper oleoresin in a 10mL centrifuge tube and centrifuge at 3000r / min for 15min, and crystallization and stratification will occur.

[0057] In addition, when the volume of ethanol added was changed to 250 mL, the obtained product also showed centrifugal stratification and crystallization.

[0058] It should be noted that the endpoints and any values ​​of the ranges disclosed in this article are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoint values ​​of each range, the endpoint values ​​of each range and the individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be regarded as specifically disclosed in this article.

[0059] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "specific implementations", or "some specific implementations" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiment of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0060] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A method for refining pepper oleoresin, characterized in that: include: The pepper oleoresin raw material is mixed with the alkaline alcohol solution, and then kept at 40-45°C for stratification, and then concentrated to obtain refined pepper oleoresin and piperine crude extract; the alcohol concentration of the alkaline alcohol solution is 70-75°, and the pH value of the system after the pepper oleoresin raw material and the alkaline alcohol solution are mixed is 8-9; the mass volume ratio of the pepper oleoresin raw material to the alkaline alcohol solution is 1: (1.5-2) g / mL.

2. The refining method according to claim 1, characterized in that The alcohol in the alkaline alcohol solution is ethanol.

3. The purification method according to claim 1 or 2, characterized in that: The method also comprises the step of crystallizing the piperine crude extract.

4. The refining method according to claim 3, characterized in that: The crystallization method comprises the following steps: dissolving the piperine crude extract in ethanol, cooling the mixture to 0°C at a rate of 8-12°C / h under a stirring speed of 25-35 r / min, and maintaining the temperature for 1.5-2.5h.

5. A pepper oleoresin, characterized in that The product is prepared by the purification method according to claim 1 or 2.

6. Pepper oleoresin according to claim 5, characterized in that The content of piperine in the pepper oleoresin is between 25-35%, the content of piperine is less than 3%, and the content of glucosylceramide is greater than 4%.

7. A preparation of pepper oleoresin, characterized in that: Including the pepper oleoresin described in claim 5 or 6.

8. The prepared pepper oleoresin according to claim 7, characterized in that: Also includes pepper essential oil and emulsifiers.

9. The prepared pepper oleoresin according to claim 8, characterized in that: The emulsifier is Tween 80 and / or propylene glycol.

10. Application of the refining method according to claim 1 or 2 in avoiding crystallization and stratification of pepper oleoresin.

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