Bioreactor for the synthesis of mixed enzymes for glufosinate-ammonium

Abstract

The invention discloses a mixed enzyme reaction device for the biological synthesis of refined glufosinate ammonium, and relates to the field of mixed enzyme biological reaction devices. The device comprises a reactor and a rotating rod extending to the bottom of the reactor, a partition component is arranged inside the reactor, a gas transmission pipeline is arranged at the bottom of the reactor, and an exhaust component is arranged at the end of the gas transmission pipeline. The neck portion of the partition component is used to limit the moving path of the material and to separate the inside of the reactor, so that the reactor is divided into an upper half and a lower half. The forward and reverse rotation of the rotating rod is coordinated to change the circulation flow direction of the material and the opening and closing state of the communicating vessel, thereby changing the exhaust position of the exhaust component and exhausting gas. When the bubbles entrained by the material are located in the upper half, the bubbles in the material can leave the material faster, while when the bubbles entrained by the material are located in the lower half, the bubbles in the material are difficult to leave, and the contact time between the bubbles and the material is prolonged, thereby satisfying the rapid and effective control required for the material when the pH value changes.

Description

Technical Field

[0001] The present invention relates to the field of mixed enzyme bioreaction equipment, and particularly to a mixed enzyme reaction equipment for the biosynthesis of glufosinate-ammonium. Background Art

[0002] Glufosinate-ammonium is a widely used herbicide that achieves the weeding effect by inhibiting the amino acid synthesis of plants. In industrial production, the biosynthesis method of glufosinate-ammonium is mainly achieved through microbial and mixed enzyme fermentation.

[0003] During the synthesis process, a bioreactor, i.e., a special reaction kettle, is mainly used for fermentation. The reactions of microorganisms, mixed enzymes, and other substances during the fermentation process usually have special requirements. For example, microorganisms and mixed enzymes usually require continuous oxygen during the reaction process to meet the aerobic conditions of microorganisms and mixed enzymes, and the pH value changed during the reaction process is adjusted by carbon dioxide;

[0004] In the existing gas injection methods, most of them inject gas from the bottom up by directly setting different gas pipelines in the reaction kettle and arranging exhaust nozzles on the surface of the gas pipelines. However, the gas injected into the reaction kettle will quickly float to the surface of the material in the form of bubbles and then discharge outward, resulting in limited contact time between the gas and the material. When the pH value needs to be quickly adjusted by carbon dioxide due to rapid changes, it is impossible to ensure that the gas can effectively and quickly reduce the pH value of the material in a short time. If the pH value cannot be effectively controlled in a short time, it may lead to the scrapping of the overall material. Summary of the Invention

[0005] In order to make up for the deficiencies of the existing technology problems, the purpose of the present invention is to solve the problems existing in the prior art: during the gas injection process of the existing reaction kettle, the contact time between the gas and the material is limited, and when special situations occur during the reaction process, the existing gas injection method is difficult to quickly adjust the pH.

[0006] To solve the problems of the existing technology, the technical solution of the present invention is as follows: it includes a reaction kettle and a rotating rod extending to the bottom of the reaction kettle, and the rotating rod is connected to an external power device. A partition component protruding upward is arranged inside the reaction kettle. A neck-like part is arranged at the center position of the partition component, and a communication component is arranged at the bottom edge of the partition component. A communication groove is opened on the surface of the communication component;

[0007] An impeller is arranged at the part where the rotating rod penetrates through the neck-like part. When the impeller rotates forward, the material is conveyed downward along the neck-like part of the partition component. When the impeller rotates reversely, the material is conveyed upward along the neck-like part. An air pipeline is arranged at the bottom of the reaction kettle, and an exhaust component is arranged at the end of the air pipeline.

[0008] Furthermore, there are two gas supply pipelines, and the ends of the two gas supply pipelines are close to each other. The two gas supply pipelines are a carbon dioxide pipeline and an oxygen pipeline respectively.

[0009] Furthermore, the exhaust assembly includes two connecting pipes respectively connected to the two gas supply pipelines. A communicating device is arranged inside each of the two connecting pipes and is respectively connected to two groups of exhaust pipes through the communicating device, so that the two exhaust pipes can be respectively connected to the two gas supply pipelines.

[0010] Furthermore, a limiting bearing is arranged at the bottom of the rotating rod, a connecting column is arranged at the bottom of the limiting bearing, and two groups of inclined support rods are movably installed on one side of the surface of the connecting column close to the two connecting pipes. The ends of the support rods extend into the communicating device and are movably connected to a plug. Moreover, the inclination directions of the two groups of support rods are opposite. The rotation angle of the connecting column is limited by the limiting bearing, and the moving modes of the support rods with opposite inclination directions are opposite when the connecting column rotates.

[0011] Furthermore, the communicating device is divided into a connecting end and a communicating cavity. The diameter of the plug is smaller than the inner diameter of the connecting end, and a rubber layer is arranged on one side of the surface of the plug close to the support rod. The diameter of the part of the plug provided with the rubber layer is larger than the inner diameter of the connecting end. The communicating state between the connecting end and the communicating cavity is changed by the movement of the plug on both sides of the communicating cavity.

[0012] Furthermore, the connecting pipes are annular pipelines, the two connecting pipes are vertically arranged, and the connecting pipe connected to the oxygen pipeline is at the bottom, the connecting pipe connected to the carbon dioxide pipeline is at the top, and the number of communicating devices inside the connecting pipe connected to the oxygen pipeline is smaller than that inside the other connecting pipe, so that the exhaust volume of the connecting pipe connected to the oxygen pipeline to the outside through the communicating device is smaller than that of the other connecting pipe.

[0013] Furthermore, the exhaust pipes are four concentric annular pipes. The four exhaust pipes are divided into two inner and outer groups. The two exhaust pipes in each group are communicated with each other. The two groups of exhaust pipes are respectively arranged at the top and bottom of the connecting pipe and are respectively communicated with the two gas supply pipelines through the communicating device and the connecting pipe. The group of exhaust pipes communicated with the oxygen pipeline is the outer group, and the group of exhaust pipes communicated with the carbon dioxide pipeline is the inner group, so as to distinguish the exhaust areas of the two groups of exhaust pipes.

[0014] Furthermore, the exhaust pipe connected to the oxygen pipeline in the gas supply pipeline is arranged close to the communicating groove of the communicating component, and the exhaust pipe connected to the carbon dioxide pipeline is arranged inside the communicating component and is horizontally higher than the communicating groove.

[0015] Furthermore, the partition component is composed of a top neck-shaped part and a bottom inclined part connected to the reactor. The connecting component is distributed in an annular manner at the bottom of the inclined part of the partition component. The connecting component is a rectangular box-shaped structure with an opening at the top, and the opening part passes through the partition component. The connecting groove is opened on the side of the connecting component close to the bottom to limit the movement path of the material in the reactor, and the neck-shaped part forms a tubular structure adapted to the impeller and forms an auger-shaped component with the impeller, and the partition component separates the reactor into two upper and lower parts.

[0016] Furthermore, a wavy circular plate is provided on the surface of the rotating rod, and the wavy circular plate is located at a position of the rotating rod close to the connecting component. A stabilizing bracket connected to the inner wall of the reactor is provided on the surface of the rotating rod between the wavy circular plate and the exhaust component. The stabilizing bracket is rotatably connected to the rotating rod through a rotating shaft. The wavy circular plate will not drive the material to rotate during rotation but will stir the material, and the wavy circular plate can block the flowing material and bubbles. The stabilizing bracket stabilizes the vibration that may be generated by the wavy circular plate during rotation of the rotating rod, thereby ensuring a stable connection with the limit bearing.

[0017] Compared with the prior art, the advantages of the present invention are as follows:

[0018] 1. The present invention limits the moving path of the material and separates the interior of the reactor by the neck-shaped part of the partition component, so that the reactor is divided into an upper half and a lower half. When the bubbles entrained by the material are located in the upper half, the bubbles in the material can leave the material faster to meet the circulation flow of the gas in the material, while when the bubbles entrained by the material are located in the lower half, the bubbles in the material are difficult to escape, so that the bubbles in the material can be in contact with the material for a long time, thereby meeting the rapid and effective control required when the pH value of the material changes.

[0019] 2. The present invention drives the rotating rod to rotate forward or reversely through a motor, and changes the opening and closing and connection status of the communicating vessel and the flow direction of the material during the rotation. The opening and closing and connection status of the communicating vessel determine the exhaust position and exhaust gas of the exhaust component. The different flow directions determine the contact time between oxygen and carbon dioxide and the material. During forward rotation, the material entrains oxygen into the upper part and circulates. The oxygen in the material can leave the material in time after the reaction to ensure that new oxygen can enter the material, thereby optimizing the reaction effect between the material and oxygen. During reverse rotation, the material entrains carbon dioxide in the lower part and is restricted by the partition component. The material is still circulating, but it is difficult for the gas to follow the circulation through the partition component, which increases the carbon dioxide bubbles in the lower part. The material in the lower part can come into contact with a large amount of carbon dioxide, thereby quickly reducing the pH value in the material. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1Schematic diagram of the overall structure of the present invention.

[0021] Figure 2 Schematic cross-sectional view of the overall structure of the present invention.

[0022] Figure 3 Schematic diagram of the structure of the separation component of the present invention.

[0023] Figure 4 Schematic diagram of the structure of the rotating rod of the present invention.

[0024] Figure 5 Schematic connection diagram of the exhaust component of the present invention.

[0025] Figure 6 Schematic diagram of the structure of the connecting column of the present invention.

[0026] Figure 7 Schematic diagram of the structure of the communicating pipe of the present invention.

[0027] Figure 8 Schematic cross-sectional view of the communicating vessel of the present invention.

[0028] Reference numerals: 1, reaction kettle; 2, rotating rod; 201, wavy circular plate; 202, limit bearing; 203, connecting column; 204, support rod; 205, plug; 206, stable support; 3, separation component; 4, neck part; 5, communicating component; 501, communicating groove; 6, impeller; 7, gas pipeline; 8, exhaust component; 801, communicating pipe; 802, communicating vessel; 803, exhaust pipe. Detailed implementation manners

[0029] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments.

[0030] As Figure 1-8As shown in the figure, the enzymatic reaction equipment for the biosynthesis of glufosinate-ammonium includes a reaction kettle 1 and a rotating rod 2 extending to the bottom of the reaction kettle 1, and the rotating rod 2 is connected to an external power device. A separating component 3 protruding upward is arranged inside the reaction kettle 1. A neck-like part 4 is arranged at the central position of the separating component 3, and a connecting component 5 is arranged at the bottom edge of the separating component 3. A wavy circular plate 201 is arranged on the surface of the rotating rod 2. The wavy circular plate 201 is located at the position where the rotating rod 2 is close to the connecting component 5. A stabilizing bracket 206 connected to the inner wall of the reaction kettle 1 is arranged on the surface of the rotating rod 2 between the wavy circular plate 201 and the exhaust component 8. The stabilizing bracket 206 is rotationally connected to the rotating rod 2 through a rotating shaft. The wavy circular plate 201 will not drive the material to rotate during rotation but will stir the material, and the wavy circular plate 201 can block the flowing material and bubbles. The stabilizing bracket 206 stabilizes the vibration that may be generated by the wavy circular plate 201 during the rotation of the rotating rod 2, so as to ensure the stable connection with the limit bearing 202. A connecting groove 501 is opened on the surface of the connecting component 5;

[0031] An impeller 6 is arranged at the part where the rotating rod 2 passes through the neck-like part 4. The separating component 3 is composed of a top neck-like part 4 and an inclined part connected to the reaction kettle 1 at the bottom. The connecting components 5 are annularly distributed at the bottom of the inclined part of the separating component 3. The connecting component 5 is of a rectangular box-shaped structure with an open top, and the opening part penetrates through the separating component 3. The connecting groove 501 is opened on one side of the connecting component 5 close to the bottom, restricting the movement path of the material in the reaction kettle 1. And the neck-like part 4 forms a tubular structure adapted to the impeller 6 and forms a screw-like component with the impeller 6. And the separating component 3 divides the reaction kettle 1 into upper and lower parts. When the impeller 6 rotates forward, the material is conveyed downward along the neck-like part 4 of the separating component 3. When the impeller 6 rotates reversely, the material is conveyed upward along the neck-like part 4;

[0032] An air delivery pipeline 7 is arranged at the bottom of the reaction kettle 1. There are two air delivery pipelines 7. The ends of the two air delivery pipelines 7 are close to each other. The two air delivery pipelines 7 are respectively a carbon dioxide pipeline and an oxygen pipeline. An exhaust component 8 is arranged at the end of the air delivery pipeline 7. The exhaust component 8 includes two connecting pipes 801 respectively connected to the two air delivery pipelines 7. A communicating device 802 is arranged inside each of the two connecting pipes 801 and is respectively connected to two groups of exhaust pipes 803 through the communicating device 802, so that the two exhaust pipes 803 can be respectively connected to the two air delivery pipelines 7. The connecting pipe 801 is an annular pipeline. The two connecting pipes 801 are vertically arranged, and the connecting pipe 801 connected to the oxygen pipeline is located at the bottom, and the connecting pipe 801 connected to the carbon dioxide pipeline is located at the top. And the number of communicating devices 802 inside the connecting pipe 801 connected to the oxygen pipeline is less than that of the communicating device 802 inside the other connecting pipe 801, so that the exhaust volume of the connecting pipe 801 connected to the oxygen pipeline to the outside through the communicating device 802 is less than that of the other connecting pipe 801;

[0033] The exhaust pipe 803 is composed of four concentric annular pipes. The four exhaust pipes 803 are divided into two groups, inner and outer. The two exhaust pipes 803 in each group are connected. The two groups of exhaust pipes 803 are respectively arranged at the top and bottom of the connecting pipe 801 and are respectively connected to the two gas transmission pipelines 7 through the communicating vessel 802 and the connecting pipe 801. Among them, the group of exhaust pipes 803 connected to the oxygen pipeline is the outer group, and the group of exhaust pipes 803 connected to the carbon dioxide pipeline is the inner group. To distinguish the exhaust areas of the two groups of exhaust pipes 803, the exhaust pipe 803 connected to the oxygen pipeline in the gas transmission pipeline 7 is arranged close to the communication groove 501 of the connecting component 5, and the exhaust pipe 803 connected to the carbon dioxide pipeline is located inside the connecting component 5 and is horizontally higher than the communication groove 501;

[0034] A limit bearing 202 is arranged at the bottom of the rotating rod 2. A connecting column 203 is arranged at the bottom of the limit bearing 202. Two groups of inclined support rods 204 are movably installed on one side of the surface of the connecting column 203 close to the two connecting pipes 801. The ends of the support rods 204 extend into the communicating vessel 802 and are movably connected to a plug 205. And the inclination directions of the two groups of support rods 204 are opposite. The rotation angle of the connecting column 203 is limited by the limit bearing 202, and the moving modes of the support rods 204 with opposite inclination directions are opposite when the connecting column 203 rotates. The communicating vessel 802 is divided into a connecting end and a communicating cavity. The diameter of the plug 205 is smaller than the inner diameter of the connecting end. And a rubber layer is arranged on one side of the surface of the plug 205 close to the support rod 204. The diameter of the part of the plug 205 provided with the rubber layer is larger than the inner diameter of the connecting end. The communicating state between the connecting end and the communicating cavity is changed by the movement of the plug 205 on both sides of the communicating cavity.

[0035] Description of the working principle:

[0036] The present invention provides power through an external power device at the top of the rotating rod 2 to drive the rotating rod 2 and the impeller 6 and the wave circular plate 201 on its surface to rotate. In the normal use state, that is, when oxygen is input, the rotating rod 2 rotates forward. When rotating forward, the rotating rod 2 drives the connecting column 203 to rotate through the limit bearing 202 and limits the rotation angle of the connecting column 203, ensuring that the rotating rod 2 will not drive the connecting column 203 to rotate infinitely during normal rotation. After the connecting column 203 rotates, it drives the support rod 204 to move the plug 205. Among them, the plug 205 in the communicating vessel 802 of the communicating pipe 801 connected to the oxygen pipeline 7 is pulled outwards, and the plug 205 in the other communicating vessel 802 is pushed inwards, making the communicating states of the two communicating vessels 802 opposite. At this time, oxygen enters the interior of the exhaust pipe 803 through the communicating vessel 802 and is discharged upwards through the exhaust pipe 803 in the form of bubbles to be mixed with the material and move upwards. During the movement, it contacts the wave circular plate 201, and the wave circular plate 201 stirs the material during the rotation process. In this process, the impeller 6 drives the material to pass through the separation component 3 into the upper half of the reaction kettle 1. Some of the bubbles entrained in the material will leave the material at this time, and the other part of the bubbles will follow the material into the communicating component 5 again and return to the lower half of the reaction kettle 1 through the communicating groove 501 for circulation, and complete the reaction with oxygen during the circulation process;

[0037] During the reaction with oxygen, the mixed enzymes and microorganisms inside the material gradually react to saturation, reducing the oxygen demand, and at the same time, by-products will be released, affecting the pH value of the material. When the operator needs to inject carbon dioxide to change the pH value according to the data reflected by the monitoring equipment, the external power device drives the rotating rod 2, and the impeller 6 and the wave circular plate 201 on its surface to rotate in reverse. During the reverse rotation process, the impeller 6 drives the material in the upper half of the reaction kettle 1 to move downward through the separation component 3 into the lower half of the reaction kettle 1. At the same time, the rotating rod 2 drives the connecting column 203 to rotate in reverse through the limit bearing 202. After the connecting column 203 rotates, it drives the support rod 204 to move the plug 205. Among them, the plug 205 in the communicating vessel 802 of the communicating pipe 801 connected to the carbon dioxide pipeline 7 is pulled outwards, and the plug 205 in the other communicating vessel 802 is pushed inwards, blocking the oxygen from entering the exhaust pipe 803. At this time, the exhaust pipe 803 discharges carbon dioxide upwards. The carbon dioxide is mixed with the material in the form of bubbles. The material moves downward and the bubbles move upward to ensure full mixing, and the rotating wave circular plate 201 stirs the material to further mix the bubbles and the material. At this time, the bubbles in the material are difficult to pass through the separation component 3 that is moving downward through the material and continuously accumulate inside the lower half of the reaction kettle 1 until the material entrained with bubbles enters the communicating groove 501 at the bottom of the communicating component 5 and moves through the communicating component 5 to the upper half of the reaction kettle 1, and the bubbles can leave the material, enabling the material in the lower half of the reaction kettle 1 to contact a large number of carbon dioxide bubbles and quickly reduce the pH value.

[0038] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A mixed enzyme reaction device for the biological synthesis of refined glufosinate ammonium, comprising a reactor (1) and a rotating rod (2) extending to the bottom of the reactor (1), wherein the rotating rod (2) is connected to an external power device, and is characterized in that: The reactor (1) is provided with an upwardly protruding partition component (3), a neck-shaped portion (4) is provided at the center of the partition component (3), a connecting component (5) is provided at the bottom of the edge of the partition component (3), and a connecting groove (501) is provided on the surface of the connecting component (5); An impeller (6) is provided at the portion of the rotating rod (2) penetrating the neck portion (4); when the impeller (6) rotates forward, the material is transported downward along the neck portion (4) of the partition assembly (3); when the impeller (6) rotates reversely, the material is transported upward along the neck portion (4); a gas delivery pipeline (7) is provided at the bottom of the reaction kettle (1); and an exhaust assembly (8) is provided at the end of the gas delivery pipeline (7); There are two gas pipelines (7), the ends of the two gas pipelines (7) are close to each other, and the two gas pipelines (7) are respectively a carbon dioxide pipeline and an oxygen pipeline; The exhaust assembly (8) comprises two connecting pipes (801) respectively connected to the two gas transmission pipelines (7); a connecting vessel (802) is provided inside the two connecting pipes (801) and the two exhaust pipes (803) are respectively connected via the connecting vessel (802); A limit bearing (202) is provided at the bottom of the rotating rod (2), a connecting column (203) is provided at the bottom of the limit bearing (202), two groups of inclined support rods (204) are movably mounted on a side of the surface of the connecting column (203) close to the two connecting pipes (801), the ends of the support rods (204) extend into the interior of the connecting vessel (802) and are movably connected to a plug (205), and the two groups of support rods (204) are inclined in opposite directions.

2. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 1, characterized in that: The communicating vessel (802) is divided into a connecting end and a connecting cavity, the diameter of the plug (205) is smaller than the inner diameter of the connecting end, a rubber layer is provided on the surface of the plug (205) close to the support rod (204), and the diameter of the portion of the plug (205) provided with the rubber layer is larger than the inner diameter of the connecting end.

3. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 1, characterized in that: The connecting pipe (801) is an annular pipeline, and the two connecting pipes (801) are arranged vertically, and the connecting pipe (801) connected to the oxygen pipeline is located at the bottom and the connecting pipe (801) connected to the carbon dioxide pipeline is located at the top, and the number of connecting vessels (802) inside the connecting pipe (801) connected to the oxygen pipeline is less than the number of connecting vessels (802) inside the other connecting pipe (801).

4. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 3, characterized in that: The exhaust pipes (803) are four concentrically arranged annular pipes. The four exhaust pipes (803) are divided into two groups, an inner group and an outer group. The two exhaust pipes (803) in each group are connected to each other. The two groups of exhaust pipes (803) are respectively arranged at the top and bottom of the connecting pipe (801) and are respectively connected to the two gas pipelines (7) through the connecting vessel (802) and the connecting pipe (801), wherein the exhaust pipes (803) connected to the oxygen pipeline are the outer group, and the exhaust pipes (803) connected to the carbon dioxide pipeline are the inner group.

5. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 4, characterized in that: The exhaust pipe (803) connected to the oxygen pipeline in the gas transmission pipeline (7) is arranged close to the connecting groove (501) of the connecting component (5), and the exhaust pipe (803) connected to the carbon dioxide pipeline is located inside the connecting component (5) and is arranged higher than the connecting groove (501) in the horizontal direction.

6. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 1, characterized in that: The partition component (3) is composed of a neck-shaped portion (4) at the top and an inclined portion at the bottom connected to the reaction kettle (1); the connecting component (5) is distributed in an annular manner at the bottom of the inclined portion of the partition component (3); the connecting component (5) is a rectangular box-shaped structure with an opening at the top, and the opening portion passes through the partition component (3); and the connecting groove (501) is provided on one side of the connecting component (5) close to the bottom.

7. The refined glufosinate-ammonium biosynthesis mixed enzyme reaction equipment according to claim 1, characterized in that: The surface of the rotating rod (2) is provided with a wavy circular plate (201), the wavy circular plate (201) being located at a position of the rotating rod (2) close to the connecting component (5), and a stabilizing bracket (206) connected to the inner wall of the reaction kettle (1) is provided at a position of the surface of the rotating rod (2) between the wavy circular plate (201) and the exhaust component (8), the stabilizing bracket (206) being rotatably connected to the rotating rod (2) via a rotating shaft.

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