Method and device for extracting collagen from bovine bone

Abstract

The invention relates to the technical field of bovine bone processing and utilization, in particular to a method and device for extracting collagen from bovine bones. Examining the bovine bones, and removing redundant meat, fascia and fat; cooking and cleaning bovine bones, and removing impurities and bone marrow; crushing the bovine bone by using a crushing assembly; collecting the cattle bone fragments, and grinding the cattle bone fragments into cattle bone powder; adding water into the cattle bone powder, and uniformly stirring by using a stirrer; a filter plate is arranged at a discharge port of the stirrer to filter the bovine bone slurry to obtain a filtrate, and meanwhile, the filtered slurry residues are collected; the collected filter pulp residues can be put into the cattle bone powder again for secondary grinding and utilization; putting the filtrate into an autoclave for hot-pressing extraction to obtain an extract; according to the present invention, the extraction liquid is subjected to the first ultrafiltration, the extraction liquid is separated through the ultrafiltration membrane, and then the second ultrafiltration is performed, such that the bovine bone is completely ground so as to avoid the waste of the bovine bone, and the extraction rate of the collagen is substantially improved through the hot pressing extraction.

Description

Technical Field

[0001] The invention relates to the technical field of bovine bone processing and utilization, and in particular to a method and device for extracting collagen from bovine bones. Background Art

[0002] Cow bones contain a large amount of collagen, so the cow bones removed from beef need to be processed and utilized. However, during the processing of cow bones, collagen cannot be extracted through general heating and conventional operations. Therefore, the efficiency of collagen extraction can be improved by using acid and enzyme methods.

[0003] However, in the aforementioned prior art, the acid method reaction is too strong and will corrode the equipment, while the enzyme method has a low protein extraction rate and high production cost, so it is impossible to extract collagen from bovine bones efficiently and at low cost. Summary of the invention

[0004] The purpose of the present invention is to provide a method and device for extracting collagen from bovine bones, so as to solve the problem that the acid method in the prior art has too strong a reaction and will corrode the equipment, and the enzyme method has a low protein extraction rate and high production cost, and therefore cannot extract collagen from bovine bones efficiently and at low cost.

[0005] To achieve the above object, the present invention provides a method for extracting collagen from cattle bones, wherein the cattle bones are inspected and excess meat, fascia and fat are removed; The beef bones are steamed and cleaned to remove impurities and marrow; Crushing the cattle bones using a crushing assembly; Collect the broken cattle bones and grind them into cattle bone powder; Add water to the beef bone meal and mix well using a blender; A filter plate is arranged at the outlet of the mixer to filter the bovine bone slurry to obtain a filtrate, and at the same time, the filtered slurry residue is collected; The collected filter pulp residue can be put back into the cattle bone powder for secondary grinding and utilization; The filtrate is placed in an autoclave for hot pressing extraction to obtain an extract; The extract is subjected to a first ultrafiltration, separated by an ultrafiltration membrane, and then subjected to a second ultrafiltration. The obtained filtrate is spray-dried to obtain bovine collagen.

[0006] Among them, in the step of adding water to the cow bone powder and stirring evenly using a blender: The amount of water added should be 2-4 times the amount of the beef bone meal.

[0007] Wherein, in the step of placing the filtrate into an autoclave for hot pressing extraction to obtain an extract: The hot pressing temperature is 120-135℃.

[0008] Wherein, in the step of placing the filtrate into an autoclave for hot pressing extraction to obtain an extract: The hot pressing time is 1-3 hours.

[0009] The present invention also provides a device for extracting collagen from bovine bones, which uses the method for extracting collagen from bovine bones described above. The device for extracting collagen from bovine bones comprises a crushing component and a grinding component, wherein the grinding component is arranged on one side of the crushing component; The crushing assembly includes a box body, a pressing unit, a pushing unit, a sealing plate, a plurality of clamping rods and a collecting bucket, wherein the pressing unit is arranged above the box body, the pushing unit is arranged at one side of the box body, the sealing plate is rotatably connected to the box body, the sealing plate is fixed to the box body through a plurality of clamping rods, and the collecting bucket is fixedly connected to the box body and is located below the box body.

[0010] Among them, the downward pressure unit includes a first bracket, a first cylinder, a downward pressure plate and a plurality of crushing cones, the first bracket is fixedly connected to the box body and is located above the box body, the first cylinder is fixedly connected to the first bracket, the output end of the first cylinder is fixedly connected to the downward pressure plate, and the plurality of crushing cones are fixedly connected to the downward pressure plate and are distributed in sequence below the downward pressure plate.

[0011] Wherein, the pushing unit includes a second cylinder and a push plate, the second cylinder is fixedly connected to the box body and is located on one side of the box body, and the output end of the second cylinder passes through the box body and is fixedly connected to the push plate.

[0012] Wherein, the grinding assembly includes a conveying plate, a shell, a clamping unit, a motor, a rotating shaft, a grinding roller and a collecting box, the conveying plate is fixedly connected to the collecting bucket and is located below the collecting bucket, the shell is placed below the conveying plate, the clamping unit is arranged above the shell, the motor is fixedly connected to the shell and is located on one side of the shell, one end of the rotating shaft is fixedly connected to the output end of the motor, the other end of the rotating shaft is fixedly connected to the grinding roller, the grinding roller is placed inside the shell, and the collecting box is placed below the shell.

[0013] Wherein, the clamping unit includes a second bracket, a third cylinder and a clamping shell, the second bracket is fixedly connected to the shell and is located above the shell, the third cylinder is fixedly connected to the second bracket, the output end of the third cylinder is fixedly connected to the clamping shell, and the clamping shell and the grinding roller are adapted to each other.

[0014] The present invention discloses a method and device for extracting collagen from cattle bones. The method comprises the following steps: inspecting cattle bones, removing excess meat, fascia and fat; steaming and cleaning cattle bones to remove impurities and bone marrow; crushing cattle bones using a crushing assembly; collecting broken cattle bones, and then grinding them into cattle bone powder; adding water to the cattle bone powder, and stirring them evenly using a mixer; setting a filter plate at the outlet of the mixer to filter the cattle bone slurry to obtain a filtrate, and collecting the filtered slurry residue; the collected slurry residue can be re-added to the cattle bone powder for secondary grinding and utilization; placing the filtrate into an autoclave for hot-pressing extraction to obtain an extract; performing a first ultrafiltration on the extract, separating the extract using an ultrafiltration membrane, and then performing a second ultrafiltration, and spray-drying the obtained filtrate to obtain cattle bone collagen. In this way, the cattle bones are crushed to improve the grinding efficiency and grinding quality; the filtrate after grinding is filtered to reduce impurities in the cattle bone slurry and improve the quality of subsequent cattle bone collagen; by collecting the filtrate residue after filtration for secondary grinding, the waste of cattle bones can be reduced, the utilization rate of cattle bones can be improved, and the production and processing costs can be reduced; the extract obtained by hot pressing extraction is ultrafiltered twice to further remove particulate impurities in the extract, thereby ensuring the quality of collagen and greatly improving the extraction rate of collagen. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below.

[0016] Figure 1 It is a schematic diagram of the structure of the device for extracting collagen from bovine bones of the present invention.

[0017] Figure 2 It is a cross-sectional view of the device for extracting collagen from bovine bones of the present invention.

[0018] Figure 3 The present invention Figure 2 AA line section view.

[0019] Figure 4 The present invention Figure 2 Enlarged view of the local structure at point B.

[0020] Figure 5 is a flowchart of the steps of the first embodiment of the present invention.

[0021] Figure 6 is a flow chart of the steps of the second embodiment of the present invention.

[0022] Figure 7 is a flowchart of the steps of the third embodiment of the present invention.

[0023] 1-box, 2-sealing plate, 3-clamping rod, 4-collecting bucket, 5-first bracket, 6-first cylinder, 7-lower pressure plate, 8-crushing cone, 9-second cylinder, 10-push plate, 11-conveying plate, 12-shell, 13-motor, 14-rotating shaft, 15-grinding roller, 16-collecting box, 17-second bracket, 18-third cylinder, 19-pressing shell. DETAILED DESCRIPTION

[0024] Embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, but should not be construed as limiting the present invention.

[0025] First embodiment: See also Figure 5 ,in Figure 5 1 is a flow chart of the steps of the first embodiment of the present invention. The present invention provides a method for extracting collagen from bovine bones, comprising the following steps: S1: Check the cattle bones and remove excess meat, fascia and fat; S2: steaming and cleaning the beef bones to remove impurities and bone marrow; S3: crushing the cattle bones using a crushing assembly; S4: collecting the broken cattle bones and grinding them into cattle bone powder; S5: Add 2 times the amount of water to the beef bone powder and mix well using a blender; S6: a filter plate is arranged at the outlet of the mixer to filter the bovine bone slurry to obtain a filtrate, and at the same time, the filtered slurry residue is collected; S7: The collected filter pulp residue can be put back into the cattle bone powder for secondary grinding and utilization; S8: placing the filtrate into an autoclave for hot pressing extraction at a temperature of 120° C. for 3 hours to obtain an extract; S9: The extract is subjected to a first ultrafiltration, and the extract is separated by an ultrafiltration membrane, and then subjected to a second ultrafiltration. The obtained filtrate is spray-dried to obtain bovine collagen.

[0026] When using a method for extracting collagen from cattle bones of the present embodiment, S1: inspect the cattle bones and remove excess meat, fascia and fat; steam and clean the cattle bones to remove impurities and bone marrow; use a crushing component to crush the cattle bones; collect the broken cattle bones and then grind them into cattle bone powder; add 2 times of water to the cattle bone powder and use a mixer to stir evenly; set a filter plate at the outlet of the mixer to filter the cattle bone slurry to obtain a filtrate, and collect the filtered filter slurry residue at the same time; the collected filter slurry residue can be re-introduced into the cattle bone powder for secondary grinding and utilization; put the filtrate into an autoclave for hot pressing extraction, the hot pressing temperature is 120°C, the hot pressing time is 3 hours, and an extract is obtained; the extract is subjected to a first ultrafiltration, the extract is separated by an ultrafiltration membrane, and then a second ultrafiltration is performed, and the obtained filtrate is spray-dried to obtain bovine bone collagen, and the collagen yield is 60%.

[0027] Second embodiment: See also Figure 6 ,in Figure 6 1 is a flow chart of the steps of the second embodiment of the present invention. The present invention provides a method for extracting collagen from bovine bones, comprising the following steps: S1: Check the cattle bones and remove excess meat, fascia and fat; S2: steaming and cleaning the beef bones to remove impurities and bone marrow; S3: crushing the cattle bones using a crushing assembly; S4: collecting the broken cattle bones and grinding them into cattle bone powder; S5: Add 4 times the amount of water to the beef bone meal and mix well using a blender; S6: a filter plate is arranged at the outlet of the mixer to filter the bovine bone slurry to obtain a filtrate, and at the same time, the filtered slurry residue is collected; S7: The collected filter pulp residue can be put back into the cattle bone powder for secondary grinding and utilization; S8: placing the filtrate into an autoclave for hot pressing extraction at a temperature of 135° C. for 1 hour to obtain an extract; S9: The extract is subjected to a first ultrafiltration, and the extract is separated by an ultrafiltration membrane, and then subjected to a second ultrafiltration. The obtained filtrate is spray-dried to obtain bovine collagen.

[0028] When using a method for extracting collagen from cattle bones of the present embodiment, S1: inspect the cattle bones and remove excess meat, fascia and fat; steam and clean the cattle bones to remove impurities and bone marrow; use a crushing assembly to crush the cattle bones; collect the broken cattle bones and then grind them into cattle bone powder; add 4 times water to the cattle bone powder and use a mixer to mix them evenly; set a filter plate at the outlet of the mixer to filter the cattle bone slurry to obtain a filtrate, and collect the filtered filter slurry residue at the same time; the collected filter slurry residue can be re-introduced into the cattle bone powder for secondary grinding and utilization; put the filtrate into an autoclave for hot pressing extraction, the hot pressing temperature is 135°C, the hot pressing time is 1 hour, and an extract is obtained; the extract is subjected to a first ultrafiltration, the extract is separated by an ultrafiltration membrane, and then a second ultrafiltration is performed, and the obtained filtrate is spray-dried to obtain bovine bone collagen, and the collagen yield is 96.8%.

[0029] Third embodiment: See also Figure 7 ,in Figure 7 1 is a flow chart of the steps of the third embodiment of the present invention. The present invention provides a method for extracting collagen from bovine bones, comprising the following steps: S1: Check the cattle bones and remove excess meat, fascia and fat; S2: steaming and cleaning the beef bones to remove impurities and bone marrow; S3: crushing the cattle bones using a crushing assembly; S4: collecting the broken cattle bones and grinding them into cattle bone powder; S5: Add 3 times the amount of water to the beef bone powder and mix well using a blender; S6: a filter plate is arranged at the outlet of the mixer to filter the bovine bone slurry to obtain a filtrate, and at the same time, the filtered slurry residue is collected; S7: The collected filter pulp residue can be put back into the cattle bone powder for secondary grinding and utilization; S8: placing the filtrate into an autoclave for hot pressing extraction at a temperature of 130° C. for 2 hours to obtain an extract; S9: The extract is subjected to a first ultrafiltration, and the extract is separated by an ultrafiltration membrane, and then subjected to a second ultrafiltration. The obtained filtrate is spray-dried to obtain bovine collagen.

[0030] When using a method for extracting collagen from cattle bones of the present embodiment, the cattle bones are inspected and excess meat, fascia and fat are removed; the cattle bones are steamed and cleaned to remove impurities and bone marrow; the cattle bones are crushed using a crushing assembly; the broken cattle bones are collected and then ground into cattle bone powder; 3 times of water is added to the cattle bone powder and stirred evenly using a blender; a filter plate is set at the discharge port of the blender to filter the cattle bone slurry to obtain a filtrate, and the filtered filter slurry residue is collected at the same time; the collected filter slurry residue can be re-introduced into the cattle bone powder for secondary grinding and utilization; the filtrate is placed in an autoclave for hot pressing extraction, the hot pressing temperature is 130° C., and the hot pressing time is 2 hours to obtain an extract; the extract is subjected to a first ultrafiltration, the extract is separated by an ultrafiltration membrane, and then a second ultrafiltration is performed, and the obtained filtrate is spray-dried to obtain bovine bone collagen, and the collagen yield is 80%.

[0031] See also Figures 1 to 4 The present invention also provides a device for extracting collagen from bovine bones, the device for extracting collagen from bovine bones comprises a crushing component and a grinding component, the grinding component is arranged on one side of the crushing component; The crushing assembly includes a box body 1, a pressing unit, a pushing unit, a sealing plate 2, a plurality of clamping rods 3 and a collecting bucket 4, the pressing unit is arranged above the box body 1, the pushing unit is arranged on one side of the box body 1, the sealing plate 2 is rotatably connected to the box body 1, the sealing plate 2 is fixed to the box body 1 through a plurality of the clamping rods 3, and the collecting bucket 4 is fixedly connected to the box body 1 and is located below the box body 1.

[0032] In this embodiment, the entire crushing assembly can be supported by the box body 1, the cattle bones are placed in the box body 1, the pressing unit is started, the cattle bones are crushed, and then the clamping rod 3 is pulled out, the sealing plate 2 is rotated to open, and the pushing unit is started to push the cattle bones out. The broken cattle bones fall into the collecting bucket 4 and fall down, thereby crushing the cattle bones, which is more convenient for subsequent grinding and improves the grinding efficiency.

[0033] Furthermore, the pressing unit includes a first bracket 5, a first cylinder 6, a lower pressing plate 7 and a plurality of crushing cones 8, the first bracket 5 is fixedly connected to the box body 1 and is located above the box body 1, the first cylinder 6 is fixedly connected to the first bracket 5, the output end of the first cylinder 6 is fixedly connected to the lower pressing plate 7, and the plurality of crushing cones 8 are all fixedly connected to the lower pressing plate 7 and are distributed in sequence below the lower pressing plate 7.

[0034] In this embodiment, the first bracket 5 supports the first cylinder 6, and the first cylinder 6 is started to drive the lower pressure plate 7 to move, and the cattle bones are crushed with the assistance of the multiple crushing cones 8.

[0035] Furthermore, the pushing unit includes a second cylinder 9 and a push plate 10 , the second cylinder 9 is fixedly connected to the box body 1 and is located on one side of the box body 1 , and the output end of the second cylinder 9 passes through the box body 1 and is fixedly connected to the push plate 10 .

[0036] In this embodiment, the second cylinder 9 is started to drive the push plate 10 to move, and push the ground beef bones out of the box 1.

[0037] Furthermore, the grinding assembly includes a conveying plate 11, a shell 12, a clamping unit, a motor 13, a rotating shaft 14, a grinding roller 15 and a collecting box 16. The conveying plate 11 is fixedly connected to the collecting bucket 4 and is located below the collecting bucket 4. The shell 12 is placed below the conveying plate 11. The clamping unit is arranged above the shell 12. The motor 13 is fixedly connected to the shell 12 and is located on one side of the shell 12. One end of the rotating shaft 14 is fixedly connected to the output end of the motor 13, and the other end of the rotating shaft 14 is fixedly connected to the grinding roller 15. The grinding roller 15 is placed inside the shell 12, and the collecting box 16 is placed below the shell 12.

[0038] In this embodiment, the crushed cattle bones are collected by the collecting bucket 4 and fall into the conveying plate 11, and then are conveyed and fall into the shell 12. At this time, the pressing unit is started to press the crushed cattle bones onto the grinding roller 15, and then the motor 13 is started to drive the grinding roller 15 to rotate through the rotating shaft 14 to grind the crushed cattle bones. The ground crushed cattle bone powder falls downward through the gap between the grinding roller 15 and the shell 12 and is collected in the collecting box 16.

[0039] Furthermore, the pressing unit includes a second bracket 17, a third cylinder 18 and a pressing shell 19, the second bracket 17 is fixedly connected to the shell 12 and is located above the shell 12, the third cylinder 18 is fixedly connected to the second bracket 17, the output end of the third cylinder 18 is fixedly connected to the pressing shell 19, and the pressing shell 19 and the grinding roller 15 are adapted to each other.

[0040] In this embodiment, the second bracket 17 supports the third cylinder 18, and the third cylinder 18 is started to drive the pressing shell 19 to press down the crushed cattle bones, pressing them against the grinding roller 15, thereby improving the grinding effect.

[0041] What is disclosed above is only one or more preferred embodiments of the present application, and cannot be used to limit the scope of rights of the present application. Ordinary technicians in this field can understand that all or part of the processes of implementing the above embodiments and equivalent changes made according to the claims of the present application are still within the scope covered by the present application.

Claims

1. A method for extracting collagen from bovine bones, characterized in that: The steps include: Inspect the cow bones and remove excess meat, fascia and fat; The beef bones are steamed and cleaned to remove impurities and marrow; Crushing the cattle bones using a crushing assembly; Collect the broken cattle bones and grind them into cattle bone powder; Add water to the beef bone meal and mix well using a blender; A filter plate is arranged at the outlet of the mixer to filter the bovine bone slurry to obtain a filtrate, and at the same time, the filtered slurry residue is collected; The collected filter pulp residue can be put back into the cattle bone powder for secondary grinding and utilization; The filtrate is placed in an autoclave for hot pressing extraction to obtain an extract; The extract is subjected to a first ultrafiltration, separated by an ultrafiltration membrane, and then subjected to a second ultrafiltration. The obtained filtrate is spray-dried to obtain bovine collagen.

2. The method for extracting collagen from bovine bones according to claim 1, characterized in that: Add water to the beef bone meal and use a blender to mix well: The amount of water added should be 2-4 times the amount of the beef bone meal.

3. The method for extracting collagen from bovine bones according to claim 2, characterized in that: In the step of placing the filtrate into an autoclave for hot pressing extraction to obtain an extract: The hot pressing temperature is 120-135°C.

4. The method for extracting collagen from bovine bones according to claim 3, characterized in that: In the step of placing the filtrate into an autoclave for hot pressing extraction to obtain an extract: The hot pressing time is 1-3 hours.

5. A device for extracting collagen from bovine bones, using the method for extracting collagen from bovine bones as claimed in claim 4, characterized in that: The device for extracting collagen from bovine bones comprises a crushing component and a grinding component, wherein the grinding component is arranged on one side of the crushing component; The crushing assembly includes a box body, a pressing unit, a pushing unit, a sealing plate, a plurality of clamping rods and a collecting bucket, wherein the pressing unit is arranged above the box body, the pushing unit is arranged at one side of the box body, the sealing plate is rotatably connected to the box body, the sealing plate is fixed to the box body through a plurality of clamping rods, and the collecting bucket is fixedly connected to the box body and is located below the box body.

6. The device for extracting collagen from bovine bones as claimed in claim 5, characterized in that: The downward pressure unit includes a first bracket, a first cylinder, a downward pressure plate and a plurality of crushing cones. The first bracket is fixedly connected to the box body and is located above the box body. The first cylinder is fixedly connected to the first bracket. The output end of the first cylinder is fixedly connected to the downward pressure plate. The plurality of crushing cones are fixedly connected to the downward pressure plate and are distributed in sequence below the downward pressure plate.

7. The device for extracting collagen from bovine bones as claimed in claim 6, characterized in that: The pushing unit includes a second cylinder and a push plate. The second cylinder is fixedly connected to the box body and is located at one side of the box body. The output end of the second cylinder passes through the box body and is fixedly connected to the push plate.

8. The device for extracting collagen from bovine bones as claimed in claim 7, characterized in that: The grinding assembly includes a conveying plate, a shell, a clamping unit, a motor, a rotating shaft, a grinding roller and a collecting box. The conveying plate is fixedly connected to the collecting bucket and is located below the collecting bucket. The shell is placed below the conveying plate. The clamping unit is arranged above the shell. The motor is fixedly connected to the shell and is located on one side of the shell. One end of the rotating shaft is fixedly connected to the output end of the motor, and the other end of the rotating shaft is fixedly connected to the grinding roller. The grinding roller is placed inside the shell, and the collecting box is placed below the shell.

9. The device for extracting collagen from bovine bones as claimed in claim 8, characterized in that: The pressing unit includes a second bracket, a third cylinder and a pressing shell. The second bracket is fixedly connected to the shell and is located above the shell. The third cylinder is fixedly connected to the second bracket. The output end of the third cylinder is fixedly connected to the pressing shell. The pressing shell and the grinding roller are adapted to each other.

Images