Rodent eye ball injector

Abstract

The application discloses a rodent eyeball injector, which comprises a main body, a needle insertion depth identification system, an injection system, and a hollow sharp needle sleeve. The needle insertion depth identification system comprises a needle insertion depth adjusting part and a needle insertion depth visual monitoring part arranged on the main body. The injection system comprises a microinjector detachably connected with the needle insertion depth adjusting part and the hollow sharp needle sleeve detachably connected with the main body and close to an injection object. The hollow sharp needle sleeve is provided with a bevel tip for forming a pre-cut. The microinjector is connected with a flat needle head for entering the eyeball for injection. The flat needle head is movably arranged through the hollow sharp needle sleeve. The rodent eyeball injector has the bevel tip and the flat needle head, and the needle insertion depth of the microinjector can be accurately controlled. The rodent eyeball injector provides a more efficient, safe, flexible and easy-to-operate tool for rodent eye drug delivery, so as to improve the quality and efficiency of related research.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of physiological equipment, and particularly relates to a rodent eyeball injector. BACKGROUND

[0002] In the field of neuroscience and ophthalmology research, local administration or drug delivery to the eye of rodents (such as mice and rats) is a key technique. These experiments are crucial for understanding the working principles of the visual system, testing new treatment methods, and evaluating the effects of drugs. However, the existing microsyringe design has some limitations, mainly in the aspects of the single shape of the needle and the lack of needle depth marking control.

[0003] The main problems in the prior art are as follows:

[0004] (1) Needle shape problem - single shape and function of the needle:

[0005] Most of the eyeball microsyringes on the market are equipped with sharp needles, which are beneficial for penetrating the cornea, but are not suitable for all types of experiments. Most existing microsyringes are equipped with only 12° beveled tip needles or flat tip needles. Beveled tip needles are very effective when penetrating the eyeball and transcorneal. However, in some experiments, such as avoiding damage to sensitive structures in the eye or desiring more uniform drug distribution, flat tip needles are more suitable. The single needle design limits the flexibility and adaptability of experimental operations.

[0006] For example, researchers may need to use flat tip needles to avoid damaging sensitive structures in the eye or to achieve more uniform drug distribution. Therefore, in recent improved experimental operations, a sharp needle is first used to form a pre-cut on the eyeball, the needle is then withdrawn, and a flat needle is then replaced to inject the sample into the pre-cut of the eyeball. Although this method avoids damage to the eyeball caused by sharp needles, the operation of replacing the needle twice is complex. The pre-cut of the eyeball formed by the first sharp needle has no obvious marker site, and the complex needle replacement operation requires the operator to look away from the pre-cut of the eyeball, which increases the difficulty of the second injection and may cause the operator to forget the original position of the pre-cut.

[0007] (2) Insufficient control of needle depth - lack of needle depth marking: The microsyringe on the market does not provide clear needle depth indication. Due to the lack of significant and effective needle depth markers, it is difficult for the operator to accurately judge and control the depth and position of the needle entering the eye. The uncertainty of blind operation not only increases the difficulty of the experiment, but also may cause unnecessary damage to the eye (tissue damage) or inaccurate drug dosage, thereby affecting the effectiveness and reliability of the experimental results.

[0008] (3) High operation complexity:

[0009] Even some advanced microsyringes are equipped with sleeve of blind stopper to assist the needle penetration depth control, but these devices usually need to estimate the needle penetration depth and cooperate with the scale to adjust the position of the blind stopper, and the operation process is easy to change the position of the blind stoper by mistake, and the adjustment process is not intuitive enough.

[0010] Therefore, the inventors propose a rodent eyeball injector based on years of experience and practice in the relevant industry to overcome the shortcomings of the prior art. SUMMARY

[0011] The rodent eyeball injector of the present application solves the technical problems of the lack of penetration and sampling functions and the lack of needle penetration depth identification in the prior art rodent eyeball microsyringe. The present application has both beveled and flat needle tips and can accurately control the needle penetration depth of the microsyringe, providing a more efficient, safe, flexible, and easy-to-operate tool for rodent eye drug delivery, thereby improving the quality and efficiency of related research.

[0012] The rodent eyeball injector of the present application is achieved by:

[0013] a main body;

[0014] a needle penetration depth identification system including a needle penetration depth adjustment part and a needle penetration depth visualization monitoring part disposed on the main body;

[0015] an injection system including a microsyringe detachably connected to the needle penetration depth adjustment part and a hollow sharp needle sleeve detachably connected to one end of the main body near the injection object, the hollow sharp needle sleeve being provided with a beveled tip for forming a pre-cut incision; the microsyringe is connected to a flat needle tip for injection into the eyeball, and the flat needle tip is movably disposed through the hollow sharp needle sleeve.

[0016] In a preferred embodiment of the present application, the needle penetration depth adjustment part includes a movement control knob, a screw rod, and a sliding nut. The movement control knob is rotationally connected to the main body and at least partially exposed to the main body. The movement control knob is used to manually adjust the position of the microsyringe needle to adjust the needle penetration depth. The screw rod is rotationally disposed in the main body, and the axial direction of the screw rod is parallel to the axial direction of the injection system. The first end of the screw rod is disposed in the movement control knob, and the screw rod can rotate synchronously with the movement control knob. The second end of the screw rod is hingedly connected to the main body. The sliding nut is fixedly sleeved on the screw rod, and the microsyringe is detachably connected to the sliding nut to move with the sliding nut.

[0017] In a preferred embodiment of the present application, a metal fixing plate is connected to the outside of the main body, at least one magnetic fixing buckle is magnetically connected to the metal fixing plate, and the position of the magnetic fixing buckle on the metal fixing plate is adjustably arranged; an injector clamping port is arranged on the magnetic fixing buckle, and the micro-injector is clamped in the injector clamping port.

[0018] In a preferred embodiment of the present application, the needle insertion depth visualization monitoring part includes a scale display part arranged on the main body, and the scale display part is arranged on one side of the metal fixing plate.

[0019] In a preferred embodiment of the present application, a transparent outer cover that can be rotatably opened or closed is connected to the main body.

[0020] In a preferred embodiment of the present application, the hollow sharp needle sleeve is connected to a needle sleeve fixator, one end of the needle sleeve fixator is detachably connected to a connecting unit arranged on the main body.

[0021] In a preferred embodiment of the present application, the needle sleeve fixator includes a half-conical sleeve, the diameter of one end of the half-conical sleeve close to the main body is larger than the diameter of the other end of the half-conical sleeve away from the main body, a connecting column is arranged on the one end of the half-conical sleeve close to the main body, a connecting thread is arranged on the connecting column, and the connecting column and the connecting thread constitute the connecting unit; the length of the half-conical sleeve is smaller than the length of the hollow sharp needle sleeve, and the side wall of the hollow sharp needle sleeve close to the main body is connected to the half-conical sleeve.

[0022] In a preferred embodiment of the present application, the hollow sharp needle sleeve is arranged in a conical sleeve structure, the diameter of one end of the hollow sharp needle sleeve close to the main body is larger than the diameter of the other end of the hollow sharp needle sleeve away from the main body; the angle of the beveled tip of the hollow sharp needle sleeve ranges from 12° to 30°.

[0023] In a preferred embodiment of the present application, the hollow sharp needle sleeve is made of stainless steel material.

[0024] In a preferred embodiment of the present application, the main body, the needle insertion depth identification system, and the injection system are all made of high-temperature and high-pressure sterilization resistant materials.

[0025] According to the above, the rodent eyeball injector of the present application has the following beneficial effects:

[0026] The present application can support multiple needle types: the hollow sharp needle sleeve with a beveled tip can realize the function of a sharp needle, combined with the flat needle connected on the microsyringe, the present application is equivalent to having both sharp and flat needles, the beveled tip is used to form a pre-cut, and the flat needle is advanced into the eyeball for injection, reducing the number of needle insertion, while avoiding the cumbersome operation of multiple needle changes, reducing the damage to the intraocular tissue.

[0027] The microsyringe and the hollow sharp needle sleeve can be detachably connected to the main body, allowing flexible switching and allowing quick replacement of the hollow sharp needle sleeve and the flat needle to adapt to different eyeball surgery needs and support multiple needle types. The present application improves the flexibility of the syringe, enabling it to adapt to more types of operations, thereby expanding the application range.

[0028] The present application can accurately control the depth of needle insertion: the needle insertion depth adjustment part ensures that the preset depth is reached for each injection, improving the safety and accuracy of eyeball injection surgery; the visual monitoring part of the needle insertion depth provides an intuitive reference for the user, facilitating real-time monitoring of the needle insertion depth and avoiding eye damage caused by excessive insertion. The present application has high repeatability, and the stable mechanical structure ensures consistency in each setting, which helps to obtain reliable experimental results or treatment effects.

[0029] The present application enhances the simplicity of operation and user-friendly design: the simple and intuitive operation interface enables even beginners to quickly master the use method, reducing training time and cost; the overall design of the device is compact and light, making it easy to carry and store, suitable for use in different laboratories or surgical environments.

[0030] The present application can improve surgical efficiency and success rate, and reduce surgery time: as the needle insertion depth can be accurately set and controlled, the time for repeated adjustment and confirmation is reduced, and the surgical process is accelerated; accurate control of the needle insertion depth effectively reduces the risk of accidental injury during surgery, improving the success rate of surgery and the safety of experimental animals.

[0031] The present application can ensure sterility and be easy to disinfect: the design of each component of the device takes into account the requirements of sterile operation, and all parts in contact with drugs can be easily disassembled, washed and autoclaved, ensuring the sterile state of the surgical environment. Disposable component option: to further improve safety, disposable needle sleeves and other critical components can be used to avoid cross-infection. BRIEF DESCRIPTION OF DRAWINGS

[0032] The following drawings are only intended to illustrate and explain the present application and do not limit the scope of the present application.

[0033] Among them:

[0034] Figure 1Front view of rodent eyeball injector of the present application.

[0035] Figure 2 Side view of rodent eyeball injector of the present application.

[0036] Figure 3 Front view of internal structure of rodent eyeball injector of the present application.

[0037] Figure 4 Side view of internal structure of rodent eyeball injector of the present application.

[0038] In the figure:

[0039] 1. main body;

[0040] 2. movement control knob;

[0041] 3. magnetic attraction fixing buckle;

[0042] 4. metal fixing plate;

[0043] 5. scale display part;

[0044] 6. outer cover;

[0045] 7. hollow sharp needle cover;

[0046] 8. needle cover fixer;

[0047] 9. screw rod;

[0048] 10. sliding nut;

[0049] 11. connecting unit. DETAILED DESCRIPTION

[0050] In order to have a clearer understanding of the technical features, objects and effects of the present application, the specific embodiments of the present application will be described with reference to the accompanying drawings.

[0051] The specific embodiments of the present invention described herein are for illustrative purposes only and should not be construed as limiting the invention in any way. Under the teachings of this invention, those skilled in the art can conceive of any possible modifications based on the invention, all of which should be considered within the scope of the invention. It should be noted that when an element is referred to as being "set on" another element, it can be directly on the other element or there may be an intervening element. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or there may be an intervening element. The terms "mounted," "connected," and "linked" should be interpreted broadly; for example, they can refer to mechanical or electrical connections, or internal communication between two elements, and can be direct or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible embodiments.

[0052] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0053] This invention aims to provide a more efficient, safe, flexible, and easy-to-use tool for ocular drug delivery in rodents, thereby improving the quality and efficiency of related research.

[0054] like Figures 1 to 4 As shown, the present invention provides a rodent eyeball injector, comprising:

[0055] Main body 1 serves as the basic framework of the entire device, supporting and connecting all other components.

[0056] The needle insertion depth marking system includes a needle insertion depth adjustment unit and a needle insertion depth visualization monitoring unit set on the main body 1. The needle insertion depth marking system can accurately control the needle insertion depth of the micro-syringe.

[0057] The injection system comprises a microsyringe detachably connected with the needle insertion depth adjusting part, and can adopt the microsyringe in the prior art, such as the Hamilton microsyringe (33G, flat needle); the injection system further comprises a hollow sharp needle sleeve 7 detachably connected to one end of the main body 1 close to the injection object, and the hollow sharp needle sleeve 7 is provided with a beveled tip for forming a pre-cut; the flat needle head for entering the eyeball for injection is movably arranged on the microsyringe and penetrates through the hollow sharp needle sleeve 7.

[0058] The present application can support multiple needle types: the hollow sharp needle sleeve 7 with a beveled tip can realize the function of a sharp needle, and in combination with the flat needle head connected to the microsyringe, the present application is equivalent to simultaneously having a sharp needle and a flat needle, the beveled tip is used to form a pre-cut, and the flat needle head is pushed into the eyeball for injection, thereby reducing the number of needle insertions and avoiding the cumbersome operation of repeatedly replacing the needle, and reducing the damage to the intraocular tissue during operation.

[0059] The microsyringe and the hollow sharp needle sleeve 7 are both detachably connected to the main body 1, and can be flexibly switched to allow quick replacement of the hollow sharp needle sleeve 7 and the flat needle head, adapt to different eyeball surgery requirements, and support multiple needle types. The present application improves the flexibility of the injector, so that it can adapt to more types of operations, thereby expanding the application range.

[0060] The present application can accurately control the needle insertion depth: the needle insertion depth adjusting part ensures that the preset depth can be reached during each injection, thereby improving the safety and accuracy of eyeball injection surgery; the visual monitoring part of the needle insertion depth provides an intuitive reference for the user, which is convenient for real-time monitoring of the needle insertion depth and avoiding damage to the eyeball due to excessive insertion. The present application has high repeatability, and the stable mechanical structure ensures the consistency of each setting, which is helpful to obtain reliable experimental results or treatment effect.

[0061] The present application enhances the simplicity of operation and user-friendly design: the simple and intuitive operation interface enables even a novice to quickly master the use method, thereby reducing the training time and cost; the overall design of the device is compact and light in weight, which is convenient for carrying and storing, and is suitable for use in different laboratories or surgical environments.

[0062] The present application can improve the surgical efficiency and success rate and reduce the operation time: since the needle insertion depth can be accurately set and controlled, the time for repeated adjustment and confirmation is reduced, and the operation process is accelerated; accurate control of the needle insertion depth effectively reduces the risk of accidental injury during surgery, thereby improving the success rate of surgery and the safety of experimental animals.

[0063] The present application can be sterile and easy to disinfect: the design of each part of the device takes into account the requirements of aseptic operation, all parts in contact with the drug can be easily disassembled, cleaned and autoclaved at high temperature and high pressure, ensuring the aseptic state of the operating environment. Disposable assembly option: to further improve safety, disposable needle cover and other key components can be selected to avoid cross infection.

[0064] Experimental verification and data support: in order to verify the effectiveness and reliability of the present application, multiple animal experiments were conducted, the results show that in the eye injection operation using the device, all samples achieve the expected needle depth, and no eye injury caused by improper operation occurs. Compared with the traditional injection device, the operation time of the device is shortened by about 30% on average, and the operation success rate is improved by about 20%. These experimental evidences not only prove the technical superiority of the present application, but also provide a solid foundation for its popularization and application.

[0065] Further, as shown in Figure 3 、 Figure 4 , the needle depth adjusting part includes a moving control knob 2, a screw rod 9 and a sliding nut 10. The moving control knob 2 is rotationally connected to the main body 1 and at least partially exposed to the main body 1. The moving control knob 2 is used to manually adjust the position of the microsyringe needle to adjust the needle depth. The screw rod 9 is rotationally arranged in the main body 1, and the axial direction of the screw rod 9 is parallel to the axial direction of the injection system. The first end of the screw rod 9 is arranged in the moving control knob 2, and the screw rod 9 can rotate synchronously with the moving control knob 2. The second end of the screw rod 9 is hingedly connected to the main body 1. The sliding nut 10 is fixedly sleeved on the screw rod 9 in the circumferential direction, and the microsyringe is detachably connected to the sliding nut 10 to move with the sliding nut 10.

[0066] The needle depth adjusting part has a fine adjustment function. The moving control knob 2 is used to adjust the position of the needle in millimeter level, so that the preset depth can be achieved in each injection.

[0067] In a specific embodiment, the screw rod 9 has a thread specification of M8, an outer diameter of 8 mm, a pitch of 1.25 mm, and a length of 100 mm. The sliding nut 10 has a thread hole diameter of 7.06 mm, an opposite side width of 13 mm, and a thickness of 6 mm.

[0068] As shown in Figure 3 、 Figure 4 , the top of the screw rod 9 is connected to the moving control knob 2 to provide a stable reference point. By rotating the moving control knob 2, the sliding nut 10 can move along its axial direction (vertical direction in Figure 3 ), thereby driving the microsyringe to move forward or backward steadily.

[0069] Further, as shown in Figure 1 、 Figure 2 ,Figure 3 , Figure 4 As shown, a metal fixing plate 4 (referring to ferromagnetic metal) is connected to the sliding nut 10 on the outside of the main body. At least one magnetic fixing buckle 3 is magnetically connected to the metal fixing plate 4, and it is generally preferred that the magnetic fixing buckles 3 be arranged in pairs. The position of the magnetic fixing buckle 3 on the metal fixing plate 4 is adjustable. A syringe clip interface is provided on the magnetic fixing buckle 3, into which a micro-syringe is clipped. Specifically, the syringe clip interface connects to the syringe barrel of the micro-syringe. One end of the injection piston is sealed and slides through the syringe barrel, while the other end of the injection piston protrudes from the main body 1 and is exposed, facilitating injection operations after adjusting the needle insertion depth.

[0070] The magnetic fastener 3 has a magnet on its back, allowing it to adhere to the metal mounting plate 4. The syringe clip of the magnetic fastener 3 has an inner diameter of 6mm, which can accommodate and fasten a glass microsyringe with an outer diameter of 6.6mm, ensuring the microsyringe is securely installed at the front of the device. (The syringe clip of the magnetic fastener 3 is made of silicone, which has a certain degree of plasticity, so the inner diameter of the syringe clip is slightly smaller than the outer diameter of the syringe to ensure reliable fixation.) In one specific embodiment, the magnetic fastener 3 has a length of 7mm along the metal mounting plate 4.

[0071] One side of the metal fixing plate 4 is fixedly connected to the sliding nut 10, and the other side is connected to the micro-injector, forming a stabilizing system that can fix the micro-injector.

[0072] By rotating the control knob 2, the sliding nut 10 can be moved along its axial direction. Figure 3 (In the vertical direction), thereby changing the position of the metal fixing plate 4, which in turn drives the micro-injector to move steadily forward or backward. This design achieves precise control of the needle insertion depth while maintaining ease of operation.

[0073] The present invention has high repeatability, and the stable mechanical structure ensures consistency in each setup, which helps to obtain reliable experimental results or therapeutic effects.

[0074] Furthermore, such as Figure 1 As shown, the needle insertion depth visualization monitoring unit includes a scale display unit 5 disposed on the main body 1. The scale display unit 5 is located on one side of the metal fixing plate 4, facilitating observation and setting of the specific needle insertion depth. In one specific embodiment, the unit of the scale display unit 5 is millimeters (mm). The needle insertion depth visualization monitoring unit realizes visual monitoring of needle insertion. The scale display unit 5 provides the user with an intuitive reference, facilitating real-time monitoring of the needle insertion depth and avoiding eye damage caused by over-insertion.

[0075] The present application introduces a complete needle insertion depth identification system, including a scale display part 5 and a needle insertion depth adjustment part. The needle insertion depth adjustment part is based on a screw nut mechanism, and the needle of the microinjector can be accurately pushed forward by moving the control knob 2. Combined with the scale display part 5, it can help researchers more accurately control the depth of the needle into the eye, reduce the risk of misoperation, and improve the consistency and safety of the experiment.

[0076] Further, as shown in Figure 1 、 Figure 2 , the main body 1 is connected with a transparent outer cover 6 that can be opened or closed by rotation. The outer cover 6 protects the internal mechanical structure while allowing the user to view the scale display part 5 (scale line) to accurately set the needle insertion depth, i.e. provides intuitive visual feedback to ensure operation accuracy.

[0077] Further, as shown in Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 , the hollow sharp needle sleeve 7 is connected to the needle sleeve holder 8, and the hollow sharp needle sleeve 7 and the needle sleeve holder 8 form an integral structure that can be detached and replaced. One end of the needle sleeve holder 8 is detachably connected to the connecting unit 11 (note that the connecting unit 11 is not connected to the screw rod 9, and there is a space interval between the two) on the main body 1. The needle sleeve holder 8 ensures that the hollow sharp needle sleeve 7 is securely installed and prevents loosening during use.

[0078] Further, as shown in Figure 4 , the needle sleeve holder 8 includes a half-conical sleeve, the diameter of the end of the half-conical sleeve close to the main body 1 is larger than the diameter of the end of the half-conical sleeve away from the main body 1, the end of the half-conical sleeve close to the main body 1 is provided with a connecting column, and the connecting column is provided with a connecting thread, and the connecting column and the connecting thread constitute the connecting unit; the length of the half-conical sleeve is smaller than the length of the hollow sharp needle sleeve 7, and the side wall of the hollow sharp needle sleeve 7 close to the main body 1 is connected with the half-conical sleeve.

[0079] In a specific embodiment, the diameter of the end of the half-conical sleeve close to the main body 1 is 7mm, the diameter of the end of the half-conical sleeve away from the main body 1 is 5.5mm, and the axial length is 30mm.

[0080] Further, the hollow sharp needle sleeve 7 is provided in a conical sleeve structure, the diameter of the end of the hollow sharp needle sleeve 7 close to the main body 1 is larger than the diameter of the end of the hollow sharp needle sleeve 7 away from the main body 1; the angle of the beveled tip of the hollow sharp needle sleeve 7 ranges from 12° to 30°, and the best choice is 12° or 30°.

[0081] In a specific embodiment, the hollow sharp needle sleeve 7 is made of stainless steel material. The total length of the hollow sharp needle sleeve 7 can be: 30 mm, 40 mm, 50 mm, 60 mm, 70 mm; the inner diameter-outer diameter of the hollow sharp needle sleeve 7 can be: ① 0.25 mm-0.35 mm, ② 0.4 mm-0.65 mm; the beak angle: 12°, 30°; more can be selected according to actual needs.

[0082] The total length, inner diameter-outer diameter and beak angle of the hollow sharp needle sleeve 7 made of stainless steel material can be selected according to actual needs to adapt to different surgical needs; and can be used with a microsyringe to quickly complete the pre-cutting and sampling operation.

[0083] Further, all components of the present application, i.e. the main body 1, the needle insertion depth identification system and the injection system, are made of high-temperature and high-pressure sterilization materials, supporting thorough cleaning and disinfection before and after repeated use. This not only ensures the hygiene and safety of use, but also reduces the cost of long-term use.

[0084] Embodiment

[0085] I. Experimental materials and equipment

[0086] Rodent eyeball injector (micro-eyeball injection device): as described above, including main body 1, movement control knob 2, magnetic attraction fixing buckle 3, metal fixing plate 4, scale display part 5, outer cover 6, hollow sharp needle sleeve 7, needle sleeve fixer 8, screw rod 9, sliding nut 10, connection unit 11, and Hamilton microsyringe (33G, flat head needle).

[0087] Experimental animals: healthy adult mice (the specific number is determined according to the experimental design), which have been adapted to the laboratory environment and have been properly anesthetized.

[0088] Drugs: isoflurane, tropicamide and other ophthalmic drugs that require micro-injection or physiological saline as a control.

[0089] Other tools: inhalation anesthesia machine, sterile operation table, microscope, disinfection supplies, etc.

[0090] II. Experimental steps

[0091] 1. Preparation stage

[0092] Selecting the needle type: Choose the appropriate needle sleeve based on the specific experimental requirements (e.g., beveled tip for subretinal injection, flat tip for intravitreal injection). Install the Hamilton microsyringe through the needle sleeve holder 8 and magnetic fixing buckle 3 to the front end of the device, ensuring secure installation without looseness, then carefully insert the 30mm long, 0.25mm-0.35mm inner and outer diameter, 12° bevel angle specification hollow sharp needle sleeve 7 through the microsyringe needle, avoiding the hollow sharp needle sleeve 7 inner wall from touching the syringe needle (flat tip), then fix the hollow sharp needle sleeve 7 on the main body 1 through the connection unit.

[0093] Calibrating the needle insertion depth: Rotate the movement control knob 2 to drive the sliding nut 10 to move along the screw rod 9, adjust the position of the metal fixing plate 4, so that the extension length of the needle of the microsyringe reaches the preset needle insertion depth. Check the scale display part 5 through the transparent cover to intuitively monitor and set the needle insertion depth, ensuring that each injection can achieve the expected effect.

[0094] Disinfection: All parts in contact with drugs must be strictly cleaned and autoclaved at high temperature and pressure to ensure the sterile state of the surgical environment. At the same time, the mouse is properly anesthetized to ensure that it remains still throughout the process.

[0095] 2. Perform injection

[0096] Positioning and fixing: Find the target injection site of the eyeball under the microscope and gently fix the mouse's head with appropriate clamps to ensure that its eyes are in the correct position.

[0097] Needle insertion operation: Hold the rodent eyeball injector in hand, slowly and steadily insert the hollow sharp needle sleeve 7 into the target site to form a pre-cut, then rotate the movement control knob 2 to slowly push the flat tip needle into the eyeball, and inject according to the pre-set depth. During this process, the operator should keep his hands stable to avoid accidental injury caused by shaking or other external disturbances.

[0098] Drug injection: Once the needle reaches the predetermined depth, gently push the microsyringe plunger to accurately inject the required dose of drug into the eye. The injection speed should be moderate to avoid sudden increase in intraocular pressure.

[0099] Needle withdrawal treatment: After completing the injection, slowly withdraw the flat tip needle by moving the control knob 2 to avoid bleeding or tissue damage caused by rapid withdrawal. When withdrawing the needle, pay attention to keep the needle direction unchanged to reduce the impact on the surrounding tissues.

[0100] 3. Postoperative treatment

[0101] Observation and Recording: Immediately after the injection is completed, observe the mouse's eye condition, including whether there is bleeding, swelling, or other abnormal phenomena. Record the specific parameters of each injection in detail, such as needle insertion depth, injection volume, injection site, and other information for subsequent analysis.

[0102] Recovery Care: Place the mouse in a warm and quiet environment and monitor its recovery until it is fully awake. For any mice that appear to be in discomfort, appropriate treatment measures should be taken in a timely manner.

[0103] Cleaning and Maintenance: After each experiment, clean the micro-ocular injection device in a timely manner, especially the parts that come into direct contact with the drug, to ensure that there is no residue affecting the next use. Regularly check and maintain the device to ensure that it is always in the best working condition.

[0104] III. Experimental Results and Discussion

[0105] Through micro-injection experiments on multiple mice, the effectiveness and reliability of the rodent ocular injector of the present invention were verified:

[0106] Safety: All samples achieved the expected needle insertion depth, and no eye damage occurred due to improper operation. This indicates that the device can effectively protect the eye structure and reduce the risk of surgery.

[0107] Accuracy: Compared with traditional injection devices, the use of the present invention reduces the operation time by an average of about 30%, and the success rate of surgery is increased by about 20%. This is due to its precise needle insertion depth control mechanism and stable mechanical structure.

[0108] Applicability: As it supports multiple types of needles and can flexibly adjust the needle insertion depth, the device is suitable for different ophthalmic experimental needs and has a wide range of applications.

[0109] IV. Conclusion

[0110] This example demonstrates the superior performance of the rodent ocular injector of the present invention in mouse ocular micro-injection drug administration experiments. It not only improves the safety and accuracy of the operation, but also simplifies the operation process, providing strong technical support for animal ophthalmic surgery research.

[0111] From the above, the rodent ocular injector of the present invention has the following beneficial effects:

[0112] The present application can support multiple needle types: hollow sharp needle sleeve with beveled tip can realize the function of sharp needle, combined with the flat needle connected on the microsyringe, the present application is equivalent to having sharp needle and flat needle at the same time, the beveled tip is used to form a pre-cut, the flat needle is pushed into the eyeball to inject, reducing the number of needle insertion, avoiding the cumbersome operation of multiple needle changes, reducing the damage to the intraocular tissue.

[0113] The microsyringe and the hollow sharp needle sleeve 7 are detachably connected to the main body, flexible switching, allowing quick replacement of the hollow sharp needle sleeve and the flat needle, adapting to different eyeball surgery needs, supporting multiple needle types. The present application improves the flexibility of the syringe, enabling it to adapt to more types of operations, thereby expanding the application range.

[0114] The present application can accurately control the depth of needle insertion: the needle insertion depth adjustment part ensures that the preset depth can be reached every time, improving the safety and accuracy of eyeball injection surgery; the visual monitoring part provides an intuitive reference for the user, facilitating real-time monitoring of the needle insertion depth and avoiding eye damage caused by excessive insertion. The present application has high repeatability, and the stable mechanical structure ensures the consistency of each setting, which helps to obtain reliable experimental results or treatment effect.

[0115] The present application enhances the simplicity of operation and user-friendly design: the simple and intuitive operation interface enables even beginners to quickly master the use method, reducing training time and cost; the overall design of the device is compact and light, easy to carry and store, suitable for use in different laboratories or surgical environments.

[0116] The present application can improve the efficiency and success rate of surgery and reduce the operation time: as the needle insertion depth can be accurately set and controlled, the time for repeated adjustment and confirmation is reduced, and the operation process is accelerated; accurate control of the needle insertion depth effectively reduces the risk of accidental injury during surgery, improves the success rate of surgery and the safety of experimental animals.

[0117] The present application can ensure sterility and be easy to disinfect: the design of each component of the device takes into account the requirements of sterile operation, all parts in contact with drugs can be easily disassembled, washed and autoclaved, ensuring the sterile state of the surgical environment. Disposable component option: to further improve safety, disposable needle sleeve and other key components can be selected to avoid cross infection.

[0118] Experimental verification and data support, in order to verify the effectiveness and reliability of the present application, a number of animal experiments were carried out, the results show that: using the device for the eyeball injection operation, all samples achieve the expected needle depth, no eye injury caused by improper operation. Compared with the traditional injection device, the operation time of the device is shortened by about 30% on average, and the operation success rate is increased by about 20%. These experimental evidences not only prove the technical superiority of the present application, but also provide a solid foundation for its popularization and application.

[0119] The above merely illustrates the specific embodiments of the present application, and is not intended to limit the scope of the present application. Any equivalent changes and modifications made by those skilled in the art without departing from the concept and principles of the present application shall fall within the scope of the present application.

Claims

1. A rodent eyeball injector, characterized in that, include: main body; The needle insertion depth marking system includes a needle insertion depth adjustment unit and a needle insertion depth visualization monitoring unit disposed on the main body; The injection system includes a micro-injector detachably connected to the needle depth adjustment unit and a hollow, sharp needle sheath detachably connected to one end of the main body near the injection target. The hollow, sharp needle sheath is provided with a beveled tip for forming a pre-incision. A flat-tipped needle for injection into the eyeball is connected to the micro-injector, and the flat-tipped needle is movably inserted through the hollow, sharp needle sheath. The needle depth adjustment unit includes a movement control knob, a screw, and a sliding nut. The movement control knob is rotatably connected to the main body and at least partially exposed thereout. The movement control knob is used to manually adjust the position of the microsyringe needle to adjust the needle depth. The screw is rotatably disposed within the main body, and the axis of the screw is parallel to the axis of the injection system. The first end of the screw passes through the movement control knob and can rotate synchronously with the movement control knob. The second end of the screw is hinged to the main body. The sliding nut is circumferentially fixedly sleeved on the screw, and the microsyringe is detachably connected to the sliding nut to move with the sliding nut.

2. The rodent eyeball injector as described in claim 1, characterized in that, A metal fixing plate located on the outside of the main body is connected to the sliding nut. At least one magnetic fixing buckle is magnetically connected to the metal fixing plate. The position of the magnetic fixing buckle on the metal fixing plate is adjustable. A syringe clip interface is provided on the magnetic fixing buckle, and the micro-syringe is clipped into the syringe clip interface.

3. The rodent eyeball injector as described in claim 2, characterized in that, The needle insertion depth visualization monitoring unit includes a scale display unit disposed on the main body, and the scale display unit is disposed on one side of the metal fixing plate.

4. The rodent eyeball injector as described in claim 1, characterized in that, A transparent outer cover that can be rotated open or snapped closed is attached to the main body.

5. The rodent eyeball injector as described in claim 1, characterized in that, The hollow, pointed needle sheath is connected to the needle sheath retainer, and one end of the needle sheath retainer is provided with a connecting unit that can be detachably connected to the main body.

6. The rodent eyeball injector as described in claim 5, characterized in that, The needle sheath retainer includes a semi-conical sleeve, the diameter of the end of the semi-conical sleeve closer to the main body is larger than the diameter of the end farther from the main body, a connecting post is provided at the end of the semi-conical sleeve closer to the main body, the connecting post is provided with a connecting thread, the connecting post and the connecting thread constitute the connecting unit; the length of the semi-conical sleeve is smaller than the length of the hollow sharp needle sheath, and the side wall of the hollow sharp needle sheath is connected to the semi-conical sleeve at the position close to the main body.

7. The rodent eyeball injector as described in claim 1, characterized in that, The hollow sharp needle sheath is designed with a conical structure, and the diameter of the end of the hollow sharp needle sheath closer to the main body is larger than the diameter of the end farther from the main body; the angle of the beveled tip of the hollow sharp needle sheath is in the range of 12° to 30°.

8. The rodent eyeball injector as described in claim 1, characterized in that, The hollow, sharp needle sheath is made of stainless steel.

9. The rodent eyeball injector as described in claim 1, characterized in that, The main body, the needle depth marking system, and the injection system are all made of high-temperature and high-pressure sterilization resistant materials.

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