A method for preparing Aconitum kusnezoffii using Yunnan Agarwood extract
By soaking the toxicity of turmeric flakes with the alcohol extract of Yunnan soil agarwood, the toxicity of turmeric flakes was successfully reduced, and the problem that existing preparation methods were difficult to effectively reduce the toxicity of turmeric flakes was solved, and the production of turmeric flakes that meet the pharmacopoeia standards was achieved.
Patent Information
- Application Number
- CN202411973541.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-30
- Publication Date
- 2025-04-08
AI Technical Summary
Caowu is highly toxic, and the existing preparation methods are difficult to effectively reduce its toxicity, resulting in improper dose control and easy to be poisoned or even death.
The scattered alkaloids were soaked in the alcohol extract of Yunnan soil agarwood. After drying, the total amount of diester-type alkaloids in the obtained scattered alkaloid products was less than 0.04%, and the content of aconitine was less than 15% of the scattered alkaloids.
The poisoning of Caowu produced by this method is significantly reduced, complies with the content standards stipulated in the Chinese Pharmacopoeia, is simple and green, and is suitable for large-scale operation and promotion.
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Figure CN119770551A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of traditional Chinese medicine processing, and particularly relates to a method for processing aconite by utilizing Yunnan agarwood extract. Background Art
[0002] Aconitum kusnezoffii Reichb is the dried root of Aconitum kusnezoffii of the Ranunculaceae family. The Chinese medicinal pieces prepared from raw Aconitum include Aconitum kusnezoffii, processed Aconitum kusnezoffii, and Aconitum leaves. The Chinese Pharmacopoeia records that the processed Aconitum kusnezoffii tastes pungent, bitter, and hot; it is highly toxic. It enters the heart, liver, kidney, and spleen meridians. Aconitum kusnezoffii has the effects of dispelling wind and dampness, warming the meridians and relieving pain. It is used for wind-cold dampness arthralgia, joint pain, cold pain in the heart and abdomen, pain caused by cold hernia, and anesthesia and pain relief. The medicinal piece Aconitum kusnezoffii leaves are a traditional Mongolian medicinal material. They are pungent, astringent, and flat in nature; they are slightly toxic. They have the effects of clearing heat, detoxifying, and relieving pain. They are used for fever due to febrile diseases, diarrhea, abdominal pain, headache, and toothache. Diester alkaloids such as aconitine, mesaconitine, and neoaconitine are the effective ingredients for Aconitum kusnezoffii to exert its effects, and are also the key ingredients that cause toxicity. After poisoning by aconite alkaloids, it affects the central nervous system, respiratory system, and cardiovascular system, and produces toxic damage to multiple organs such as the heart, brain, liver, and kidneys, leading to toxic symptoms such as arrhythmia, hypotension, and abnormal heart function. Manifestations include numbness of the whole body and limbs, salivation, nausea, and vomiting. In severe cases, limb convulsions, unconsciousness, and difficulty breathing can eventually lead to death.
[0003] Aconitum kusnezoffii is highly toxic, and improper dosage control can cause poisoning or even death. Therefore, during use, its safety must be considered first. However, due to different origins and processing methods, the principles of reducing toxicity and maintaining efficacy are also different. There are many methods for processing Aconitum kusnezoffii, including boiling, steaming or a combination of boiling and steaming, steaming after steaming, steaming after steaming, vinegar processing, terminalia chebula processing, licorice processing, children's urine processing, yogurt processing, etc. Developing processing methods that effectively reduce the toxicity of Aconitum kusnezoffii has always been a research hotspot. Summary of the invention
[0004] The purpose of the present invention is to provide a method for preparing Aconitum kusnezoffii by using Yunnan Aquilaria sinensis extract.
[0005] The present invention provides a method for processing Aconitum kusnezoffii, the method comprising the following steps:
[0006] (1) Extraction: extracting Yunnan agarwood powder with alcohol to obtain Yunnan agarwood alcohol extract;
[0007] (2) Soaking: Slice the Aconitum kusnezoffii and soak it in the Yunnan Aquilaria alcohol extract;
[0008] (3) Air-drying: Air-dry the Aconitum kusnezoffii slices until the moisture content is less than 12.0%, thereby obtaining the processed Aconitum kusnezoffii slices.
[0009] Furthermore, in step (1), the mass volume ratio of the Yunnan agarwood powder to the alcohol is 1-5 kg: 5-15 L.
[0010] Furthermore, in step (1), the mass volume ratio of the Yunnan agarwood powder to the alcohol is 3kg:10L.
[0011] Furthermore, in step (1), the alcohol is edible alcohol with a concentration of 95%; and the extraction method is heating reflux method or percolation method.
[0012] Furthermore, in step (1), the heating reflux condition is: extraction at 80-100° C. for 2-6 hours.
[0013] Furthermore, in step (1), the heating reflux condition is: extraction at 90° C. for 4 hours.
[0014] Furthermore, in step (2), the soaking time is 24 to 72 hours.
[0015] Furthermore, in step (3), the drying conditions are: drying under natural light, a temperature of 10 to 30° C., and maintaining ventilation for 3 days.
[0016] The present invention also provides a processed Aconiti Kusnezoffii product, which is the processed Aconiti Kusnezoffii product prepared according to the above method.
[0017] Furthermore, the total amount of diester-type alkaloids in the processed Radix Aconiti Kusnezoffii is less than 0.04%, and the content of aconitine is less than 15% of the content of Radix Aconiti Kusnezoffii.
[0018] The present invention discloses for the first time a method for preparing Radix Aconiti Kusnezoffii using an alcohol extract of Yunnan native agarwood. The method soaks Radix Aconiti Kusnezoffii slices in an alcohol extract of Yunnan native agarwood for 24 to 72 hours, and then dries the slices to obtain a Yunnan native agarwood processed Radix Aconiti Kusnezoffii. The total amount of diester-type alkaloids in the Radix Aconiti Kusnezoffii prepared by the method is less than 0.04%, and the content of aconitine is less than 15% of the Radix Aconiti Kusnezoffii content. The method is simple and green, can be operated on a large scale, and is easy to promote.
[0019] Obviously, according to the above contents of the present invention, in accordance with common technical knowledge and customary means in the art, without departing from the above basic technical ideas of the present invention, other various forms of modification, replacement or change may be made.
[0020] The above contents of the present invention are further described in detail below through specific implementation methods in the form of examples. However, this should not be understood as the scope of the above subject matter of the present invention being limited to the following examples. All technologies realized based on the above contents of the present invention belong to the scope of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] Figure 1 The present invention discloses a processing process of the processed Yunnan native agarwood product (Yunnan processed Aconitum kusnezoffii). DETAILED DESCRIPTION
[0022] The raw materials and equipment used in the present invention are all known products, which are obtained by purchasing commercially available products.
[0023] Yunnan Aquilaria (Excoecaria acerifolia Didr.) was collected from Longpan Township, Yulong County, Lijiang City, Yunnan Province (100°4'16"E, 27°5'32"N). Combined with the morphological characteristics of the sample, it was identified as Yunnan Aquilaria by Professor Chen Yang of Guiyang Medical University.
[0024] Aconitum kusnezoffii Reichb was purchased from Luoshiwan Chinese herbal medicine market in Yunnan Province with batch number D10011806001 and was identified as the dried root of Aconitum kusnezoffii Reichb by Professor Chen Yang of Guiyang Medical University.
[0025] The present invention is based on Figure 1 The processing process shown is used to prepare the processed products of Aconiti Kusnezoffii.
[0026] The edible alcohol used in the specific implementation manner is purchased from Shanghai Testing Technology Service Department and is edible alcohol with a concentration of 95%.
[0027] Example 1: Method for preparing Aconitum kusnezoffii with Yunnan Aquilaria sinensis extract
[0028] 1. Experimental methods
[0029] (1) Extraction: 3.0 kg of dried Yunnan agarwood powder was crushed and then heated and refluxed with edible alcohol (10.0 L) for extraction (extraction temperature: 90° C.; extraction time: 4 hours), and filtered to obtain the alcohol extract of Yunnan agarwood;
[0030] (2) Soaking: Cut the Aconitum kusnezoffii into slices less than 5 mm thick and soak them in Yunnan Aquilaria sinensis extract for 24 hours;
[0031] (3) Drying: Take out the Aconitum kusnezoffii slices and dry them under natural light for 3 days at a temperature of 26° C. with ventilation until the moisture content is less than 12.0%, thereby obtaining the processed Yunnan Agarwood (Yunnan processed Aconitum kusnezoffii).
[0032] 2. Content detection
[0033] According to the method of the Chinese Pharmacopoeia, samples of Radix Aconiti Kusnezoffii (control) and Yunnan Agarwood processed products (Yunnan Radix Aconiti Kusnezoffii) were prepared: the samples were crushed in a multifunctional grinder, passed through a No. 4 sieve (65 mesh), and 2.0 g of sample powder was accurately weighed on a 1 / 10,000 electronic analytical balance. The powder was placed in a 50 mL conical flask, 1 mL of ammonia test solution was added to allow the liquid to penetrate into the powder, and then 50 mL of a mixed solution of isopropanol and ethyl acetate (1:1) was added, and ultrasonic treatment (25°C) was performed for 30 minutes, cooled, shaken, and filtered. After the primary filtrate was taken out after extraction, 25 mL of the subsequent filtrate was collected, and the solvent was removed by vacuum pressure at 40°C until dry. The remaining medicinal material residues were added with 3 mL of a mixed solution of isopropanol and dichloromethane (1:1) and ultrasonically dissolved to prepare the aconitum alkaloid extract, which was aspirated using a disposable syringe needle, filtered with a 0.22 μm microporous filter membrane, and 10 μL was injected for detection.
[0034] After testing, the contents of aconitine, mesaconitine and aconitine in the control sample were 0.158 mg / g, 0.395 mg / g and 1.075 mg / g respectively, and the total content of the three diester alkaloids was 0.16%. The contents of aconitine, mesaconitine and aconitine in the processed Yunnan Aquilaria sinensis (Yunnan processed Aconiti Kusnezoffii) were 0.0195 mg / g, 0.1399 mg / g and 0.2333 mg / g respectively, and the total content of the three diester alkaloids was 0.039%, which was in line with the content regulations of processed Aconiti Kusnezoffii in the Chinese Pharmacopoeia (not to exceed 0.040%). Among them, the content of aconitine was reduced to 12.34% (0.0195 mg / g) of the content level of Aconiti Kusnezoffii.
[0035] Example 2: Method for preparing Aconitum kusnezoffii with Yunnan Aquilaria sinensis extract
[0036] 1. Experimental methods
[0037] (1) Extraction: 3.0 kg of dried Yunnan agarwood powder was crushed and then heated and refluxed with edible alcohol (10.0 L) for extraction (extraction temperature: 90° C.; extraction time: 4 hours), and filtered to obtain the alcohol extract of Yunnan agarwood;
[0038] (2) Soaking: Cut the Aconitum kusnezoffii into slices less than 5 mm thick and soak them in Yunnan Aquilaria sinensis extract for 48 hours;
[0039] (3) Drying: Take out the Aconitum kusnezoffii slices and dry them under natural light for 3 days at a temperature of 26° C. with ventilation until the moisture content is less than 12.0%, thereby obtaining the processed Yunnan Agarwood (Yunnan processed Aconitum kusnezoffii).
[0040] 2. Content detection
[0041] According to the method of the Chinese Pharmacopoeia, samples of Radix Aconiti Kusnezoffii (control) and Yunnan Agarwood processed products (Yunnan Radix Aconiti Kusnezoffii) were prepared: the samples were crushed in a multifunctional grinder, passed through a No. 4 sieve (65 mesh), and 2.0 g of sample powder was accurately weighed on a 1 / 10,000 electronic analytical balance. The powder was placed in a 50 mL conical flask, 1 mL of ammonia test solution was added to allow the liquid to penetrate into the powder, and then 50 mL of a mixed solution of isopropanol and ethyl acetate (1:1) was added, and ultrasonic treatment (25°C) was performed for 30 minutes, cooled, shaken, and filtered. After the primary filtrate was taken out after extraction, 25 mL of the subsequent filtrate was collected, and the solvent was removed by vacuum pressure at 40°C until dry. The remaining medicinal material residues were added with 3 mL of a mixed solution of isopropanol and dichloromethane (1:1) and ultrasonically dissolved to prepare the aconitum alkaloid extract, which was aspirated using a disposable syringe needle, filtered with a 0.22 μm microporous filter membrane, and 10 μL was injected for detection.
[0042] After testing, the contents of aconitine, mesaconitine and aconitine in the processed Yunnan native agarwood product (Yunnan processed aconitine) were 0.0116 mg / g, 0.1235 mg / g and 0.2128 mg / g respectively, and the total content of the three diester-type alkaloids was 0.035%, which was in line with the content requirements of the processed aconitine in the "Chinese Pharmacopoeia" (not to exceed 0.040%). Among them, the content of aconitine was reduced to 7.34% (0.0195 mg / g) of the aconitine content level. Comparative Example 1: Method for processing aconitine with Yunnan native agarwood extract
[0043] 1. Experimental methods
[0044] (1) Extraction: 3.0 kg of dried Yunnan agarwood powder was crushed and then heated and refluxed with edible alcohol (10.0 L) for extraction (extraction temperature: 90° C.; extraction time: 4 hours), and filtered to obtain an alcohol extract of Yunnan agarwood; the solvent was removed under reduced pressure, and the extract was dissolved in 10.0 L of warm water to obtain an aqueous solution of the alcohol extract of Yunnan agarwood;
[0045] (2) Soaking: Cut the Aconitum kusnezoffii into slices less than 5 mm thick and soak them in Yunnan Aquilaria sinensis extract for 24 hours;
[0046] (3) Drying: Take out the Aconitum kusnezoffii slices and dry them under natural light for 3 days at a temperature of 26° C. with ventilation until the moisture content is less than 12.0%, thereby obtaining the processed Yunnan Agarwood (Yunnan processed Aconitum kusnezoffii).
[0047] 2. Content detection
[0048] According to the method of the Chinese Pharmacopoeia, samples of Radix Aconiti Kusnezoffii (control) and Yunnan Agarwood processed products (Yunnan Radix Aconiti Kusnezoffii) were prepared: the samples were crushed in a multifunctional grinder, passed through a No. 4 sieve (65 mesh), and 2.0 g of sample powder was accurately weighed on a 1 / 10,000 electronic analytical balance. The powder was placed in a 50 mL conical flask, 1 mL of ammonia test solution was added to allow the liquid to penetrate into the powder, and then 50 mL of a mixed solution of isopropanol and ethyl acetate (1:1) was added, and ultrasonic treatment (25°C) was performed for 30 minutes, cooled, shaken, and filtered. After the primary filtrate was taken out after extraction, 25 mL of the subsequent filtrate was collected, and the solvent was removed by vacuum pressure at 40°C until dry. The remaining medicinal material residues were added with 3 mL of a mixed solution of isopropanol and dichloromethane (1:1) and ultrasonically dissolved to prepare the aconitum alkaloid extract, which was aspirated using a disposable syringe needle, filtered with a 0.22 μm microporous filter membrane, and 10 μL was injected for detection.
[0049] After testing, the contents of aconitine, mesaconitine and aconitine in the processed Yunnan agarwood aqueous solution were 0.0481 mg / g, 0.2586 mg / g and 0.5181 mg / g respectively, and the total content of the three diester alkaloids was 0.08%, exceeding the content of processed aconitum in the Chinese Pharmacopoeia (not to exceed 0.040%). Among them, the content of aconitine was reduced to 30.44% (0.0481 mg / g) of the content level of aconitum.
[0050] The above results show that the content of diester-type alkaloids in the processed Yunnan agarwood product (Yunnan processed Aconitum kusnezoffii) obtained under the processing conditions of Examples 1 and 2 (i.e., the Yunnan agarwood extract is an alcohol extract) meets the pharmacopoeia standards, while the content of diester-type alkaloids in the processed Yunnan agarwood product (Yunnan processed Aconitum kusnezoffii) obtained under the processing conditions of Control Example 1 (i.e., the Yunnan agarwood extract is an aqueous solution of an alcohol extract) does not meet the pharmacopoeia standards.
[0051] In summary, the present invention provides a method for preparing Aconitum kusnezoffii using Yunnan Agarwood extract. The present invention discloses for the first time a method for preparing Aconitum kusnezoffii using an alcohol extract of Yunnan Agarwood, wherein Aconitum kusnezoffii slices are soaked in an alcohol extract of Yunnan Agarwood for 24 to 72 hours, dried, and a Yunnan Agarwood processed Aconitum kusnezoffii product is obtained. The total amount of diester-type alkaloids in the Aconitum kusnezoffii prepared by the method is less than 0.04%, and the content of aconitine is less than 15% of the Aconitum kusnezoffii content level. The present method is simple and green, can be operated on a large scale, and is easy to promote.
Claims
1. A method for processing Aconitum kusnezoffii, characterized in that: The method comprises the following steps: (1) Extraction: extracting Yunnan agarwood powder with alcohol to obtain Yunnan agarwood alcohol extract; (2) Soaking: Slice the Aconitum kusnezoffii and soak it in the Yunnan Aquilaria alcohol extract; (3) Air-drying: Air-dry the Aconitum kusnezoffii slices until the moisture content is less than 12.0%, thereby obtaining the processed Aconitum kusnezoffii slices.
2. The method according to claim 1, characterized in that In step (1), the mass volume ratio of the Yunnan agarwood powder to the alcohol is 1-5 kg: 5-15 L.
3. The method according to claim 2, characterized in that In step (1), the mass volume ratio of the Yunnan agarwood powder to the alcohol is 3kg:10L.
4. The method according to claim 1, characterized in that: In step (1), the alcohol is edible alcohol with a concentration of 95%; and the extraction method is heating reflux method or percolation method.
5. The method according to claim 4, characterized in that In step (1), the heating reflux condition is: extraction at 80-100° C. for 2-6 hours.
6. The method according to claim 5, characterized in that In step (1), the heating reflux condition is: extraction at 90° C. for 4 hours.
7. The method according to claim 1, characterized in that In step (2), the soaking time is 24 to 72 hours.
8. The method according to claim 1, characterized in that In step (3), the drying conditions are: drying under natural light, a temperature of 10 to 30° C., and maintaining ventilation for 3 days.
9. A processed product of Aconite, characterized in that: It is a processed Aconiti Kusnezoffii product prepared according to the method described in any one of claims 1 to 8.
10. The processed Aconite Radix Kusnezoffii product according to claim 9, characterized in that: The total amount of diester alkaloids in the processed product of Aconiti Kusnezoffii is less than 0.04%, and the content of aconitine is less than 15% of the content of Aconiti Kusnezoffii.