A kind of preparation method of glutinous rice geranium oil

Abstract

The invention discloses a glutinous rice leaf oil and a preparation method thereof. The glutinous rice leaf is crushed and screened, and extracted twice by an aqueous enzyme method. After the extraction, solid-liquid separation is performed, and reduced pressure concentration is performed. Finally, caprylic decanoic acid glyceride is added and stirred to obtain a supernatant to obtain the glutinous rice leaf oil. The advantages of the invention are that the glutinous rice leaf oil prepared by the invention has a high yield, the extract is rich in aroma, the glutinous rice aroma is obvious, and there is no chlorophyll and solvent residue, and the glutinous rice leaf oil can be directly used in cigarette popping beads. The glutinous rice leaf oil tobacco flavor is prepared by an extraction method, and the components and characteristics of the glutinous rice leaf are maintained to the greatest extent, providing a new way for the glutinous rice leaf. At the same time, the developed flavor has a significant effect on the formation and improvement of the style of cigarette products.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco flavor extraction, in particular to a preparation method of glutinous rice geranium oil. Background Art

[0002] Glutinous rice is a perennial dwarf aromatic herb of the genus Acanthaceae. Its leaves emit a smell similar to glutinous rice and are a favorite beverage ingredient of the Dai people. The aroma of glutinous rice is mellow and leisurely, and is loved by most people. It has great development value as a flavor raw material.

[0003] At present, the application of glutinous rice fragrant leaves is mainly based on direct use of leaves: for example, CN101138375A discloses a method for making glutinous rice fragrant tea, and CN115836699A discloses a rice flower fragrant tea and its preparation method, both of which directly mix glutinous rice fragrant leaves with materials such as tea leaves. The existing methods for extracting glutinous rice fragrant leaves are mainly based on water or ethanol extraction. For example, "Study on the style of dissolving glutinous rice fragrant components in ethanol-water system [J]. Anhui Agricultural Science, 2018, 46(22): 3." studied the problems related to dissolving glutinous rice fragrant leaf components using ethanol and water as solvents.

[0004] At present, the glutinous rice aroma in tobacco products is mainly achieved by glutinous rice aroma extraction or synthetic flavoring, but the effect is not good. The aroma of glutinous rice leaves is naturally strong, and the quality of the products obtained by the existing extraction methods is insufficient. The use of organic solvents for extraction has problems such as dissolved residues and the presence of chlorophyll, and it is difficult to have a good application in tobacco products. Therefore, it is currently necessary to improve the extraction process of glutinous rice leaves to obtain extracts with excellent aroma quality, which will greatly promote the development of glutinous rice flavor tobacco products. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a method for preparing glutinous rice geranium oil with excellent aroma quality.

[0006] In order to solve the above technical problems, the technical solution of the present invention is:

[0007] A method for preparing glutinous rice geranium oil comprises the following steps:

[0008] S1: After the glutinous rice fragrant leaves are dried, they are crushed with a high-speed grinder, and then sieved with a 20-40 mesh sieve to obtain glutinous rice fragrant leaves powder, which is sealed for later use;

[0009] S2: Mix the glutinous rice leaf powder treated in step S1 with water in a mass ratio of 1:(6-10), add 1%-3% of a biological enzyme, and perform a first extraction at a temperature of 45° C.-55° C.; after the extraction, perform solid-liquid separation to obtain a filtrate A;

[0010] S3: Mix the filtrate A obtained by extraction in step S2 with the unextracted glutinous rice bay leaf powder, perform a second extraction at a temperature of 45° C. to 55° C., and perform solid-liquid separation after the extraction to obtain an extract B;

[0011] S4: inactivating the extract B obtained in step S3 at 90° C. for 1 h, and concentrating under reduced pressure to obtain a concentrated solution C;

[0012] S5: Add caprylic acid glyceride to the concentrated solution C obtained in step S4 and stir for 10 minutes. Centrifuge at 6000 r / min for 10 minutes, take the supernatant and obtain glutinous rice geranium oil.

[0013] Preferably, the biological enzyme in step S2 is one or more of protease, cellulase, pectinase and amylase.

[0014] Preferably, the mass ratio of the glutinous rice bay leaf powder and water processed in step S2 is 1:8.

[0015] Preferably, in step S2, the first extraction time is 2 to 4 hours and the second extraction time is 1 to 2 hours.

[0016] Preferably, in step S2, the first extraction time is 2 hours and the second extraction time is 1 hour.

[0017] Preferably, the first extraction and the second extraction temperature are 50°C.

[0018] Preferably, the reduced pressure concentration conditions in step S4 are: vacuum degree 0.06Mpa-0.08Mpa, temperature 65°C-75°C.

[0019] Preferably, the amount of caprylic decanoic acid glyceride used in step S5 is 16% to 20% of the amount of feed.

[0020] The glutinous rice geranium oil prepared by the method can be used in cigarette beads of tobacco products.

[0021] Beneficial effects of the present invention:

[0022] The glutinous rice fragrant leaf oil prepared by the invention has a high yield, rich extract aroma, obvious glutinous rice aroma, no chlorophyll and solvent residue, and can be directly used in cigarette popping beads. The glutinous rice fragrant leaf oil smoke flavor is prepared by extraction, which maintains the components and characteristics of the glutinous rice fragrant leaf to the greatest extent, provides a new way for the glutinous rice fragrant leaf, and at the same time, the developed flavor has a significant effect on the formation and improvement of the style of cigarette products. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 A process flow chart is provided for preparing a process using the method of the present invention. DETAILED DESCRIPTION

[0024] The specific embodiments of the present invention are further described below in conjunction with the accompanying drawings. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0025] Refer to the attached Figure 1 As shown, a method for preparing glutinous rice geranium oil comprises the following steps:

[0026] S1: After the glutinous rice fragrant leaves are dried, they are crushed with a high-speed grinder, and then sieved with a 20-40 mesh sieve to obtain glutinous rice fragrant leaves powder, which is sealed for later use;

[0027] In step S1, the glutinous rice fragrant leaves are dried to make them brittle and easy to crush. After high-speed physical crushing, the internal organizational structure is destroyed, and the powdered raw materials can fully contact with the solvent, which is conducive to the outflow of the internal fragrance substances and shortening the extraction cycle. The sealing bag can prevent the fragrance from volatilizing.

[0028] S2: Mix the glutinous rice leaf powder treated in step S1 with water in a mass ratio of 1:(6-10), add 1%-3% of a biological enzyme, and perform a first extraction at a temperature of 45° C.-55° C.; after the extraction, perform solid-liquid separation to obtain a filtrate A;

[0029] S3: Mix the filtrate A obtained by extraction in step S2 with the unextracted glutinous rice bay leaf powder, perform a second extraction at a temperature of 45° C. to 55° C., and perform solid-liquid separation after the extraction to obtain an extract B;

[0030] S4: inactivating the extract B obtained in step S3 at 90° C. for 1 h, and concentrating under reduced pressure to obtain a concentrated solution C;

[0031] S5: Add caprylic acid glyceride to the concentrated solution C obtained in step S4 and stir, stir for 10 minutes, centrifuge for 10 minutes at 6000r / min, take the supernatant, and obtain glutinous rice geranium oil. During the enzyme extraction process, the larger the amount of solvent and the longer the extraction time, the higher the yield, but the material yield will increase with the increase in the amount of solvent and the extension of the extraction time. Therefore, it is important to select suitable conditions considering the process cycle. Generally, the higher the extraction temperature, the higher the extraction efficiency, but too high a temperature will inactivate the enzyme, so the selection of the extraction temperature is also very important.

[0032] In step S2, the biological enzyme is one or more of protease, cellulase, pectinase and amylase.

[0033] The mass ratio of the glutinous rice bay leaf powder and water processed in step S2 is 1:8.

[0034] In step S2, the first extraction time is 2 to 4 hours and the second extraction time is 1 to 2 hours.

[0035] In step S2, the first extraction time is 2 hours and the second extraction time is 1 hour.

[0036] The first extraction and the second extraction temperature are 50°C.

[0037] The reduced pressure concentration conditions in step S4 are: vacuum degree 0.06Mpa-0.08Mpa, temperature 65°C-75°C.

[0038] In step S5, the amount of caprylic decanoate used is 16% to 20% of the amount of material added.

[0039] The glutinous rice geranium oil obtained by the preparation method of glutinous rice geranium oil can be applied in cigarette popping beads of tobacco products.

[0040] Embodiment 1:

[0041] Determination of enzyme types in the preparation method of glutinous rice geranium oil.

[0042] A method for preparing glutinous rice geranium oil comprises the following steps:

[0043] S1: The glutinous rice bay leaves are first dried at 50°C for 60 minutes, then crushed with a high-speed grinder, and then sieved with a 20-40 mesh sieve. The sieved tobacco powder is placed in a sealed bag for later use.

[0044] S2: Mix the glutinous rice leaf powder treated in step S1 with water in a mass ratio of 1:8, add protease, cellulase, pectinase, amylase, and complex enzyme respectively for extraction, and perform the first extraction at a temperature of 50° C. for 2 h; after the extraction, perform solid-liquid separation to obtain filtrate A;

[0045] S3: Mix the filtrate A obtained by extraction in step S2 with the unextracted glutinous rice bay leaf powder, perform a second extraction at a temperature of 50° C. for 1 hour, and perform solid-liquid separation after the extraction to obtain an extract B;

[0046] S4: inactivating the extract B obtained in step S3 at 90° C. for 1 h, and concentrating under reduced pressure to obtain a concentrated solution C;

[0047] S5: Add caprylic acid glyceride to the concentrated solution C obtained in step S4 and stir for 10 minutes, centrifuge at 6000 r / min for 10 minutes, take the supernatant, and obtain glutinous rice bay leaf oil. Calculate the yield of glutinous rice bay leaf and perform sensory evaluation on it.

[0048] The results show that: cellulase and amylase extracts have strong irritation, heavy foreign smell, unobvious aroma enhancement effect, and low yield, protease and pectinase extracts have moderate irritation, strong foreign smell, unclean aftertaste, and medium yield, and composite enzyme extracts have moderate irritation, foreign smell, and have aroma enhancement effect on cigarettes, and high yield. Therefore, the present invention uses composite enzymes as the biological enzyme.

[0049] Embodiment 2:

[0050] Determination of the material-liquid mass ratio in the preparation method of glutinous rice geranium oil.

[0051] The difference from Example 1 is that in step S2, the glutinous rice bay leaf powder and water are mixed in a mass ratio of 1:4, 1:6, 1:8, 1:10, and 1:12 respectively, and the biological enzyme is 3% of a complex enzyme. The rest is the same, and the yield of the glutinous rice bay leaves is calculated and the sensory evaluation is performed.

[0052] The results show that when the extraction temperature and time are fixed, the aroma concentration of glutinous rice geranium oil increases with the increase of the material-liquid ratio of the two extractions; when the material-liquid ratio reaches 1:8, the aroma concentration no longer changes significantly with the increase of the ratio. Based on comprehensive consideration of the preparation cost and process cycle, the present invention selects the material-liquid mass ratio of the two extractions as 1:8 as the optimal parameter.

[0053] Embodiment 3:

[0054] Determination of enzyme dosage in the preparation method of glutinous rice geranium oil.

[0055] The difference from Example 1 is that in step S2, glutinous rice bay leaf powder and water are mixed in a mass ratio of 1:8, and the compound enzyme feeding ratio is 1%, 2%, 3%, 4% and 5% respectively for extraction. The rest is the same, and the yield of glutinous rice bay leaves is calculated and the sensory evaluation is performed.

[0056] The results show that the aroma concentration of the extract prepared at a concentration of 1% is low and the aroma enhancement effect is not obvious; the aroma concentration of the extract prepared at a concentration of 1-2% is medium and the aroma enhancement effect is average; the aroma concentration of the extract prepared at a concentration of 3% is high and the aroma enhancement effect is good; the aroma concentration of the extract prepared at a concentration of 4-5% is high and the aroma enhancement effect is good. Taking comprehensive considerations, the present invention selects 3% as the amount of enzyme added for extraction.

[0057] Embodiment 4:

[0058] Determination of the amount of capric glyceride in the preparation method of glutinous rice geranium oil.

[0059] The difference from Example 1 is that 10%, 12%, 14%, 16%, 18% and 20% of caprylic decanoic acid glyceride are added for extraction respectively, and the rest are the same in calculating the yield of glutinous rice bay leaves and conducting sensory evaluation.

[0060] The results show that the aroma concentration of the extract prepared at a concentration of 10-12% is low and the aroma enhancement effect is not obvious; the aroma concentration of the extract prepared at a concentration of 14-16% is medium and the aroma enhancement effect is average; the aroma concentration of the extract prepared at a concentration of 16-20% is high and the aroma enhancement effect is good. Considering comprehensively, 16-20% is selected as the dosage of caprylic decanoic acid glyceride.

[0061] The preparation method of the invention is simple, safe and environmentally friendly, the raw materials are easily available, and industrial production can be realized. Through aqueous enzyme extraction, the glutinous rice bay leaf can be fully extracted to obtain the glutinous rice bay leaf oil that can be preserved, is not easy to corrupt and does not contain chlorophyll. The glutinous rice bay leaf oil is made into popping beads and applied in cigarettes, which can give the tobacco products a glutinous rice aroma and enrich the aroma characteristics of tobacco products; its aroma characteristics are coordinated with the style of cigarettes, the aftertaste is pure, the impurities and irritation of cigarettes are significantly reduced, and the overall aroma quality of cigarettes is enhanced.

[0062] The embodiments of the present invention are described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions and variations of these embodiments are made without departing from the principles and spirit of the present invention, and still fall within the scope of protection of the present invention.

Claims

1. A method for preparing glutinous rice geranium oil, characterized in that: The following steps are involved: S1: After the glutinous rice fragrant leaves are dried, they are crushed with a high-speed grinder, and then sieved with a 20-40 mesh sieve to obtain glutinous rice fragrant leaves powder, which is sealed for later use; S2: Mix the glutinous rice leaf powder treated in step S1 with water in a mass ratio of 1:(6-10), add 1%-3% of a biological enzyme, and perform a first extraction at a temperature of 45° C.-55° C.; after the extraction, perform solid-liquid separation to obtain a filtrate A; S3: Mix the filtrate A obtained by extraction in step S2 with the unextracted glutinous rice bay leaf powder, perform a second extraction at a temperature of 45° C. to 55° C., and perform solid-liquid separation after the extraction to obtain an extract B; S4: inactivating the extract B obtained in step S3 at 90° C. for 1 h, and concentrating under reduced pressure to obtain a concentrated solution C; S5: Add caprylic acid glyceride to the concentrated solution C obtained in step S4 and stir for 10 minutes. Centrifuge at 6000 r / min for 10 minutes, take the supernatant and obtain glutinous rice geranium oil.

2. The preparation method of glutinous rice geranium oil according to claim 1, characterized in that: In step S2, the biological enzyme is one or more of protease, cellulase, pectinase and amylase.

3. The preparation method of glutinous rice geranium oil according to claim 1, characterized in that: The mass ratio of the glutinous rice bay leaf powder and water processed in step S2 is 1:

8.

4. The preparation method of glutinous rice geranium oil according to claim 1, characterized in that: In step S2, the first extraction time is 2 to 4 hours and the second extraction time is 1 to 2 hours.

5. The preparation method of glutinous rice geranium oil according to claim 1, characterized in that: In step S2, the first extraction time is 2 hours and the second extraction time is 1 hour.

6. The method for preparing glutinous rice geranium oil according to claim 1, wherein: The first extraction and the second extraction temperature are 50°C.

7. The method for preparing glutinous rice geranium oil according to claim 1, characterized in that: The reduced pressure concentration conditions in step S4 are: vacuum degree 0.06Mpa-0.08Mpa, temperature 65°C-75°C.

8. The method for preparing glutinous rice geranium oil according to claim 1, wherein: In step S5, the amount of caprylic decanoate used is 16% to 20% of the amount of material added.

9. Glutinous rice geranium oil prepared by the method according to any one of claims 1 to 8.

10. Use of the glutinous rice geranium oil according to claim 9 in tobacco products.

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