Frozen animal lymphatic tissue liquid separation device and working method thereof

Abstract

The frozen animal lymphatic tissue liquid separation device comprises a separation box, a mounting shaft is arranged at the top end in the separation box through a first motor, the first motor drives the mounting shaft to rotate, the middle of the mounting shaft is rotationally connected with a liquid containing pipe through a fixing plate, and a through hole and a liquid outlet hole are formed in the bottom of the liquid containing pipe; a push rod is arranged in the through hole, one end of the push rod extends into the liquid containing pipe to be connected with the bottom of the isolation filter plate, a sealing rod is arranged in the liquid outlet hole, one end of the sealing rod extends into the liquid containing pipe to be connected with the bottom of the isolation filter plate, and a decomposition component, a centrifugal component, a liquid separation component and a cleaning component are sequentially arranged in the separation box in the rotating direction of the fixing plate. The decomposition component is used for unfreezing the frozen lymph tissue, the centrifugal component is used for driving the liquid containing pipe to rotate, the liquid separation component is used for separating liquid in the liquid containing pipe, and the cleaning component is used for cleaning the liquid containing pipe and the isolation filter plate. The device is convenient to operate, and improves the integrity of lymphoid tissue cells.

Description

Technical Field

[0001] The invention belongs to the technical field of cell separation, and in particular relates to a frozen animal lymphatic tissue liquid separation device and a working method thereof. Background Art

[0002] Animal lymphoid tissue plays an important role in experimental medicine, mainly used to study the function of the immune system and the development of disease models. Experiments on animal lymphoid tissue also include lymphocyte transformation tests, which are a method for evaluating the state of cellular immune function in the body. By adding appropriate stimuli during in vitro T cell culture and observing the transformation of lymphocytes, the state of cellular immune function in the body can be evaluated. The application of these experimental techniques provides an effective means for studying the response and regulatory mechanism of the immune system. However, when separating lymphocytes, the existing technology requires the experimenter to mechanically decompose the lymphoid tissue, and after decomposition, the lymphoid tissue needs to be placed in a reaction tube. After the reaction, the solid lymphoid tissue needs to be separated. Most of the existing separation methods simply separate the lymphoid tissue from the liquid through a filter or other components, and there will be a lot of liquid left inside the lymphoid tissue. Directly discarding it will cause a waste of liquid, making the existing technology cumbersome to operate and low in separation efficiency.

[0003] Furthermore, animal lymphatic tissue contains liquid substances such as lymph, and in order to perform cell separation on the liquid inside the lymphatic tissue, the animal lymph needs to be frozen when obtaining the animal lymphatic tissue. When experiments are required, the existing methods mostly use a cutting machine to cut the frozen animal lymphatic tissue after it is slightly softened, and after cutting, the lymphatic tissue needs to be placed in a container. The experiment can only be performed after the lymphatic tissue is completely thawed. Therefore, the existing methods waste a lot of time when performing liquid separation on frozen animal lymph, and the lymphatic tissue is constantly transferred in various containers, which makes the frozen lymphatic tissue easily contaminated, thereby causing the failure of the liquid separation of lymphatic tissue cells.

[0004] Based on this, the present invention provides a frozen animal lymphatic tissue liquid separation device and a working method thereof, which can eliminate the disadvantages of the existing devices. Summary of the invention

[0005] Technical problem to be solved: In response to the above technical problem, the present invention provides a frozen animal lymphatic tissue liquid separation device and a working method thereof, which can effectively solve the shortcomings of the prior art of complicated separation operation and low separation efficiency.

[0006] Technical solution: In the first aspect, the present invention provides a frozen animal lymphatic tissue liquid separation device, comprising a separation box, a mounting shaft is provided at the top of the separation box through a first motor, the first motor drives the mounting shaft to rotate, the middle part of the mounting shaft is rotatably connected to a liquid filling tube through a fixed plate, a through hole and a liquid outlet are provided at the bottom of the liquid filling tube, a push rod is provided in the through hole, one end of the push rod extends to the interior of the liquid filling tube and is connected to the bottom of the isolation filter plate, a sealing rod is provided in the liquid outlet, one end of the sealing rod extends to the interior of the liquid filling tube and is connected to the bottom of the isolation filter plate, a decomposition component, a centrifugal component, a liquid separation component and a cleaning component are sequentially provided inside the separation box along the rotation direction of the fixed plate, the decomposition component is used to thaw the frozen lymphatic tissue, the centrifugal component is used to drive the liquid filling tube to rotate, the liquid separation component is used to separate the liquid in the liquid filling tube, and the cleaning component is used to clean the liquid filling tube and the isolation filter plate.

[0007] Preferably, the decomposition component includes a decomposition tube, the bottom of the decomposition tube is a decomposition net, the bottom of the decomposition net is connected to the output end of the electric push rod through a support tube that passes through the decomposition tube, a heating component is provided inside the support tube, and the heating component is electrically connected to an external control component, the electric push rod is arranged at the top end of the separation box, a mounting tube is provided at the output end of the electric push rod, a fixed decomposition plate is provided at the bottom end of the mounting tube, the outer edge of the fixed decomposition plate is slidably connected to the inner wall of the decomposition tube, the upper end of the fixed decomposition plate is connected to the inner top end of the separation box through a plurality of support rods, a first box door is provided on the side wall of the separation box located at the decomposition tube, and a rotating component for driving the liquid-filled tube to rotate is provided inside the separation box.

[0008] Furthermore, the rotating assembly includes a friction ring, which is sleeved on the outer wall of the liquid-containing tube, and a first friction wheel is provided inside the separation box, and the first friction wheel is frictionally pressed against the friction ring, and the first friction wheel is provided at one end of a first mounting shaft, and a plurality of first rotating plates are rotatably provided on the first mounting shaft, and the first rotating plates are fixedly provided inside the separation box, and a first driven wheel is provided at the other end of the first mounting shaft, and the first driven wheel is connected to the first driving wheel through a first belt, and the first driving wheel is fixedly provided on the output end of the second motor, and the second motor is fixedly provided at the bottom end inside the separation box.

[0009] Preferably, the centrifugal component includes a second friction wheel, the second friction wheel is fixedly arranged at one end of a second mounting shaft, a plurality of second rotating plates are rotatably arranged on the second mounting shaft, the second rotating plates are fixedly arranged inside the separation box, a second driven wheel is arranged at the other end of the second mounting shaft, the second driven wheel is transmission-connected to the second driving wheel through a second belt, the second driving wheel is fixedly arranged on the output end of the second motor, a first sealing plate is arranged above the second friction wheel at a position corresponding to the position of the liquid filling tube, the first sealing plate is fixedly arranged on the inner side wall of the separation box, and the lower surface of the first sealing plate is in close contact with the upper end of the liquid filling tube.

[0010] Preferably, the liquid separating component includes a first guide plate, which is arranged at the bottom end of the separation box and corresponds to the other end of the push rod. A top column is provided between the push rod and the first guide plate, and the top column is connected to the push rod through a reset spring. A liquid collecting pipe is provided on one side of the first guide plate corresponding to the position of the liquid outlet. The liquid collecting pipe is provided on the side wall of the separation box, and a drain pipe is provided at the bottom end of the liquid collecting pipe. One end of the drain pipe extends to the outside of the separation box. A correction mechanism is provided inside the separation box corresponding to the position of the first guide plate, and the correction mechanism is used to correct the position of the liquid filling pipe.

[0011] Furthermore, the correction mechanism includes a correction friction ring and a correction plate, the correction plate is arranged above one end of the first guide plate, and is connected to the inner wall of the separation box through a plurality of spring telescopic rods, a correction friction ring is fixedly provided on the outer wall of the liquid filling tube at a position corresponding to the correction plate, a plane notch is provided on the correction friction ring, rollers are provided at both ends of the plane notch, the arc surface of the correction friction ring is frictionally pressed against the correction plate, a second sealing plate is provided above the first guide plate at a position corresponding to the position of the liquid filling tube, the bottom end of the second sealing plate is at the same height as the upper end of the liquid filling tube, and the second sealing plate is fixedly provided on the inner wall of the separation box.

[0012] Furthermore, the cleaning component includes a cleaning nozzle, which is fixedly arranged inside the separation box, and a plurality of spray holes are arranged at the bottom end of the cleaning nozzle. The water inlet of the cleaning nozzle is connected with the water outlet of an external water supply device through a water inlet pipe, and a control component is arranged at the connection between the water outlet of the water inlet pipe and the water inlet of the cleaning nozzle. The control component is used to control the switch of the water inlet of the cleaning nozzle. A second guide plate is arranged under the cleaning nozzle, and the second guide plate is arranged at the bottom end of the separation box and is located directly below the top column. A collecting box is arranged between the second guide plate and the bottom of the separation box, and a filter mesh plate is arranged inside the collecting box.

[0013] Furthermore, the control component includes a ball valve, the water inlet of the ball valve is connected to the water outlet of the water inlet pipe, the water outlet of the ball valve is connected to the water inlet of the cleaning nozzle, the ball valve is fixedly arranged at the top of the separation box through a support frame, a third friction wheel is provided at one end of the valve stem of the ball valve, a friction plate is frictionally attached to the third friction wheel, and the friction plate is fixedly arranged on the upper surface of the fixed plate.

[0014] Preferably, a scraper frame is provided below the cleaning nozzle, one end of the scraper frame is fixedly arranged on the inner wall of the separation box, and the lower surface of the scraper frame is in close contact with the upper end surface of the liquid filling tube.

[0015] In a second aspect, the present invention provides a working method of the frozen animal lymphatic tissue liquid separation device according to the first aspect, comprising the following steps: S1, placing a small volume of coarse material in the decomposition tube, adding decomposition liquid into the liquid tube, starting the electric push rod, immersing the decomposition tube into the decomposition liquid in the liquid tube, turning on the heating component in the support tube, allowing the decomposition liquid to complete the initial decomposition of the lymphatic tissue, starting the second motor to drive the liquid tube to rotate at a low speed, after the initial decomposition is completed, the electric push rod drives the decomposition tube to rise, and the electric push rod and the decomposition net crush the small volume of coarse material into fine lymphatic tissue and drop it on the isolation filter plate; S2, the first motor drives the liquid-filling tube to rotate 90 degrees for the first time, the liquid-filling tube rotates to the bottom of the first sealing plate and is in close contact with the bottom end of the first sealing plate, and the second friction wheel drives the liquid-filling tube to rotate at high speed through the friction ring; S3, the first motor drives the liquid-filling tube to rotate 90 degrees for the second time, the two rollers at the plane notch are in close contact with the correction plate, the liquid outlet is aligned with the liquid collecting tube, the isolation filter plate pushes the refined lymphatic tissue to move, and under the action of the isolation filter plate and the second sealing plate, the refined lymphatic tissue is squeezed, and the decomposition liquid in the liquid-filling tube is discharged into the liquid collecting tube through the liquid outlet, and the liquid collecting tube discharges the decomposition liquid through the discharge pipe; S4. The first motor drives the liquid-filling tube to rotate 90 degrees for the third time. The bottom end of the top column is in close contact with the upper end of the second guide plate. The scraper frame scrapes the lymphatic tissue on the upper end of the isolation filter plate. The friction plate and the third friction wheel are in close contact with each other, so that the third friction wheel drives the ball to rotate. The external water supply device supplies water to the inside of the water inlet pipe. The cleaning nozzle sprays water through the spray hole to clean the isolation filter plate and the inside of the liquid-filling tube.

[0016] Beneficial effects: The present invention sequentially arranges a decomposition component, a centrifugal component, a liquid separation component and a cleaning component inside the separation box along the rotation direction of the fixed plate, so that the corresponding process can be completed after the first motor drives the liquid-filling tube to rotate 90 degrees each time, making the operation simple, and firstly decomposes the small volume of coarse material by the decomposition liquid, and then the small volume of coarse material is squeezed into fine lymphatic tissue by the cooperation of the decomposition tube and the fixed decomposition plate, and then the decomposition liquid and the fine lymphatic tissue are separated by centrifugation, thereby effectively avoiding the direct use of fine cutting to destroy the lymphatic tissue cells, and improving the integrity of the lymphatic tissue cells. The liquid-filling tube, the isolation filter plate, the push rod and the top column are respectively used in conjunction with the decomposition component, the centrifugal component and the liquid separation component to improve the separation efficiency, and after the separation is completed, the liquid-filling tube and the isolation filter plate are cleaned by the cleaning component for subsequent use, thereby increasing the practicality of the frozen animal lymphatic tissue liquid separation device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the overall structure of a frozen animal lymphatic tissue liquid separation device of the present invention; Figure 2 This is a schematic diagram of the structure of an electric push rod in a frozen animal lymphatic tissue liquid separation device of the present invention; Figure 3 This is a schematic diagram of the support tube structure in a frozen animal lymphatic tissue liquid separation device of the present invention; Figure 4 This is a schematic structural diagram of a second friction wheel and a first sealing plate in a frozen animal lymphatic tissue liquid separation device of the present invention; Figure 5 It is a schematic diagram of the structure of a correction plate and a liquid collecting pipe in a frozen animal lymphatic tissue liquid separation device of the present invention; Figure 6 It is a schematic diagram of the liquid discharge state of a liquid-filling tube in a frozen animal lymphatic tissue liquid separation device of the present invention; Figure 7 This is a schematic diagram of the position of a ball valve in a frozen animal lymphatic tissue liquid separation device of the present invention; Figure 8 This is a schematic diagram of the structure of a scraper frame in a frozen animal lymphatic tissue liquid separation device of the present invention; Fig. 9 This is a schematic diagram of the structure of a liquid-filled tube in a frozen animal lymphatic tissue liquid separation device of the present invention; Serial numbers in the figure: 11, separation box, 12, mounting shaft, 13, first motor, 14, fixed plate, 15, liquid filling pipe, 16, isolation filter plate, 17, push rod, 18, top column, 19, reset spring, 20, sealing rod, 21, friction ring, 22, correction friction ring, 23, decomposition tube, 24, support tube, 25, electric push rod, 26, fixed decomposition plate, 27, first friction wheel, 28, second motor, 29, second friction wheel, 30, first sealing plate, 31, correction plate, 32, spring telescopic rod, 33, first guide plate, 34, liquid collecting pipe, 35, second sealing plate, 36, scraper frame, 37, second guide plate, 38, cleaning nozzle, 39, water inlet pipe, 40, ball valve, 41, third friction wheel, 42, friction plate, 43, collecting box. DETAILED DESCRIPTION

[0018] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments: Example 1

[0019] like Figure 1-9 As shown, a frozen animal lymphatic tissue liquid separation device comprises a separation box 11, wherein a mounting shaft 12 is provided at the top of the separation box 11 through a first motor 13, wherein the first motor 13 drives the mounting shaft 12 to rotate, wherein the middle portion of the mounting shaft 12 is rotatably connected to a liquid-filling tube 15 through a fixed plate 14, wherein a through hole and a liquid outlet are provided at the bottom of the liquid-filling tube 15, wherein a push rod 17 is provided in the through hole, wherein one end of the push rod 17 extends to the inside of the liquid-filling tube 15 and is connected to the bottom of an isolation filter plate 16, wherein a sealing rod 20 is provided in the liquid outlet, wherein one end of the sealing rod 20 extends to the inside of the liquid-filling tube 15 and is connected to the bottom of the isolation filter plate 16, wherein a decomposition component, a centrifugal component, a liquid separation component and a cleaning component are sequentially provided inside the separation box 11 along the rotation direction of the fixed plate 14, wherein the decomposition component is used for thawing the frozen lymphatic tissue, the centrifugal component is used for driving the liquid-filling tube 15 to rotate, the liquid separation component is used for separating the liquid in the liquid-filling tube 15, and the cleaning component is used for cleaning the liquid-filling tube 15 and the isolation filter plate 16.

[0020] The above-mentioned decomposition component includes a decomposition tube 23, the bottom end of which is a decomposition net. The decomposition tube 23 and the decomposition net are both made of heat-conducting materials. The bottom of the decomposition net is connected to the output end of the electric push rod 25 through a support tube 24 that penetrates the decomposition tube 23. A heating component is provided inside the support tube 24. The heating component heats the decomposition net and the decomposition tube 23, but the temperature will not damage the lymphatic tissue cells. The heating component is electrically connected to the external control component. The electric push rod 25 is arranged at the top end of the separation box 11. A mounting tube is provided at the output end of the electric push rod 25. A fixed decomposition plate 26 is provided at the bottom end of the mounting tube. The outer edge of the fixed decomposition plate 26 is slidably connected to the inner wall of the decomposition tube 23. The upper end of the fixed decomposition plate 26 is connected to the inner top of the separation box 11 through a plurality of support rods. A first box door is provided on the side wall of the separation box 11 located at the decomposition tube 23. A rotating component for driving the liquid-filled tube 15 to rotate is provided inside the separation box 11. When in use, the softened lymphatic tissue is cut into small-volume coarse materials by an external cutting component, and then the first box door is opened to place the small-volume coarse materials in the decomposition tube 23, and then the heating component in the support tube 24 is controlled by the external control component to start, and the decomposition net and the decomposition tube 23 are both made of heat-conducting materials, so that the heating component heats the decomposition net and the decomposition tube 23, but the temperature will not damage the cells inside the lymphatic tissue, and at the same time, decomposition liquid is added to the liquid-filled tube 15. After the small-volume coarse materials in the decomposition tube 23 are thawed, the electric push rod 25 is started, and the output end of the electric push rod 25 drives the decomposition tube 2 3 moves, so that the decomposition tube 23 is immersed in the decomposition liquid in the liquid-filling tube 15, so that the decomposition liquid preliminarily decomposes the lymphatic tissue, and at the same time drives the liquid-filling tube 15 to rotate at a low speed through the rotating assembly, thereby accelerating the decomposition of the lymphatic tissue by the decomposition liquid. After the preliminary decomposition is completed, the output end of the electric push rod 25 drives the decomposition tube 23 to rise, so that the small volume of coarse material in the decomposition tube 23 is closely attached to the bottom end of the fixed decomposition plate 26, so that the small volume of coarse material is crushed into fine lymphatic tissue under the action of the electric push rod 25 and the decomposition net, and the fine lymphatic tissue falls on the upper end of the isolation filter plate 16 for separation experiments.

[0021] The rotating assembly includes a friction ring 21, which is sleeved on the outer wall of the liquid-filling tube 15. A first friction wheel 27 is provided inside the separation box 11. The first friction wheel 27 is frictionally attached to the friction ring 21. The first friction wheel 27 is provided at one end of a first mounting shaft. A plurality of first rotating plates are rotatably provided on the first mounting shaft. The first rotating plates are fixedly provided inside the separation box 11. A first driven wheel is provided at the other end of the first mounting shaft. The first driven wheel is connected to the first driving wheel through a first belt. The first driving wheel is fixedly provided on the output end of the second motor 28. The second motor 28 is fixedly provided at the bottom end inside the separation box 11. When in use, when it is necessary to preliminarily decompose the small volume of coarse material, the second motor 28 is started. The second motor 28 drives the first friction wheel 27 to rotate through the first belt. Since the first friction wheel 27 is frictionally attached to the friction ring 21, the first friction wheel 27 drives the liquid-filling tube 15 to rotate at a low speed through the friction ring 21, thereby accelerating the decomposition of the lymphatic tissue by the decomposition liquid.

[0022] The above-mentioned centrifugal component includes a second friction wheel 29, which is fixed at one end of a second mounting shaft, and a plurality of second rotating plates are rotatably provided on the second mounting shaft, and the second rotating plates are fixed inside the separation box 11, and a second driven wheel is provided at the other end of the second mounting shaft, and the second driven wheel is connected to the second driving wheel through a second belt, and the second driving wheel is fixed on the output end of the second motor 28, and a first sealing plate 30 is provided above the second friction wheel 29 at a position corresponding to the position of the liquid filling tube 15, and the first sealing plate 30 is fixedly provided on the inner side wall of the separation box 11, and the lower surface of the first sealing plate 30 is in close contact with the upper end of the liquid filling tube 15.

[0023] The above-mentioned liquid separation component includes a first guide plate 33, which is arranged at the bottom end of the separation box 11 and corresponds to the other end of the push rod 17. A top column 18 is provided between the push rod 17 and the first guide plate, and the top column 18 is connected to the push rod 17 through a reset spring 19. A liquid collecting pipe 34 is provided on one side of the first guide plate 33 corresponding to the position of the liquid outlet. The liquid collecting pipe 34 is arranged on the side wall of the separation box 11, and a drain pipe is connected to the bottom end of the liquid collecting pipe 34. One end of the drain pipe extends to the outside of the separation box 11. A correction mechanism is provided at the position of the separation box 11 corresponding to the first guide plate 33. The correction mechanism is used to correct the position of the liquid filling pipe 15.

[0024] The correction mechanism includes a correction friction ring 22 and a correction plate 31. The correction plate 31 is arranged above one end of the first guide plate 33 and is connected to the inner wall of the separation box 11 through a plurality of spring telescopic rods 32. A correction friction ring 22 is fixedly provided on the outer wall of the liquid filling tube 15 at a position corresponding to the correction plate 31. A plane notch is provided on the correction friction ring 22, and rollers are provided at both ends of the plane notch. The arc surface of the correction friction ring 22 is frictionally tightly arranged with the correction plate 31. A second sealing plate 35 is provided above the first guide plate 33 at a position corresponding to the position of the liquid filling tube 15. The bottom end of the second sealing plate 35 is at the same height as the upper end of the liquid filling tube 15. The second sealing plate 35 is fixedly arranged on the inner wall of the separation box 11. During use, after the decomposition liquid in the liquid-filling tube 15 has completed the centrifugal decomposition of the fine lymphatic tissue, the first motor 13 drives the liquid-filling tube 15 to rotate 90 degrees again, so that the liquid-filling tube 15 rotates to the bottom end of the second sealing plate 35. During this process, the correction friction ring 22 first contacts the correction plate 31. When the arc surface on the correction friction ring 22 is released from the correction plate 31, the correction friction ring 22 is rotated by the liquid-filling tube 15, and the correction friction ring 22 and the correction plate 31 are tightly pressed against each other, so that the correction friction ring 22 rotates. When one end of the flat notch on the correction friction ring 22 is pressed against one side of the correction plate 31, under the action of a plurality of spring telescopic rods 32, the two rollers at the flat notch are pressed against the correction plate 31, so that the correction friction ring 22 no longer rotates, and at the same time, the bottom end of the liquid-filling tube 15 is released. The liquid hole is aligned with the liquid collecting pipe 34, and the top column 18 is in close contact with the inclined surface of the first guide plate 33. When the installation shaft 12 drives the liquid filling pipe 15 to rotate, one end of the top column 18 moves on the inclined surface of the first guide plate 33, so that the top column 18 pushes the isolation filter plate 16 to move through the push rod 17, and the isolation filter plate 16 pushes the refined lymphoid tissue to move. When one end of the top column 18 is in close contact with the uppermost end of the first guide plate 33, under the action of the upper end of the isolation filter plate 16 and the bottom end of the second sealing plate 35, the refined lymphoid tissue is squeezed, thereby separating the liquid generated inside the refined lymphoid tissue. At the same time, the sealing rod 20 is separated from the liquid outlet hole, and the decomposition liquid inside the liquid filling pipe 15 is discharged into the liquid collecting pipe 34 through the liquid outlet hole. The liquid collecting pipe 34 discharges the decomposition liquid through the drain pipe, which is convenient for subsequent experimental research.

[0025] The above-mentioned cleaning component includes a cleaning nozzle 38, which is fixedly arranged inside the separation box 11. A plurality of spray holes are arranged at the bottom end of the cleaning nozzle 38. The water inlet of the cleaning nozzle 38 is connected with the water outlet of the external water supply device through the water inlet pipe 39. A control component is arranged at the connection between the water outlet of the water inlet pipe 39 and the water inlet of the cleaning nozzle 38. The control component is used to control the switch of the water inlet of the cleaning nozzle 38. A second guide plate 37 is arranged below the cleaning nozzle 38. The second guide plate 37 is arranged at the bottom end of the separation box 11 and is located directly below the top column 18. A collecting box 43 is arranged between the second guide plate 37 and the bottom of the separation box 11, and a filter screen is arranged inside the collecting box 43. During use, after the decomposition liquid in the liquid filling pipe 15 is drained, the first motor 13 drives the liquid filling pipe 15 to rotate 90 degrees again, so that the liquid filling pipe 15 rotates to the bottom of the cleaning nozzle 38. During this process, the bottom end of the top column 18 is firstly in close contact with the inclined surface of the second guide plate 37. Under the guidance of the second guide plate 37, the top column 18 pushes the isolation filter plate 16 to slide inside the liquid filling pipe 15 through the push rod 17. When one end of the top column 18 is in close contact with the upper end of the second guide plate 37, the isolation filter plate 16 and one end of the liquid filling pipe 15 are in the same plane. Subsequently, water is supplied to the inside of the water inlet pipe 39 through the external water supply component. The water inlet pipe 39 transports the water source to the inside of the cleaning nozzle 38. The cleaning nozzle 38 sprays water through a plurality of spray holes, so that the isolation filter plate 16 and the inside of the liquid filling pipe 15 are cleaned. A control component for controlling the switch of the input end of the cleaning nozzle 38 is provided at the connection between the output end of the water inlet pipe 39 and the input end of the cleaning nozzle 38.

[0026] The above-mentioned control component includes a ball valve 40, the water inlet of the ball valve 40 is connected to the water outlet of the water inlet pipe 39, the water outlet of the ball valve 40 is connected to the water inlet of the cleaning nozzle 38, the ball valve 40 is fixedly arranged at the top end of the separation box 11 through a support frame, and a third friction wheel 41 is provided at one end of the valve stem of the ball valve 40, and a friction plate 42 is frictionally tightly attached to the third friction wheel 41, and the friction plate 42 is fixedly arranged on the upper surface of the fixed plate 14. During use, when the fixed plate 14 drives the liquid-filled tube 15 to rotate 90 degrees to below the cleaning nozzle 38, the friction plate 42 and the third friction wheel 41 are rubbed tightly together, so that the third friction wheel 41 drives the ball to rotate 90 degrees, so that the ball valve 40 is in a conducting state. When cleaning is completed, the first motor 13 drives the liquid-filled tube 15 to rotate 90 degrees to below the decomposition tube 23 again. During this process, the friction plate 42 continues to rub tightly together with the third friction wheel 41, so that the ball rotates 90 degrees again, so that the ball valve 40 is in a closed state. At the same time, a waste water tank for collecting waste water is also provided at the bottom end of the separation box 11 and the bottom end of the decomposition tube 23, which is not drawn in the figure.

[0027] A scraper frame 36 is provided below the cleaning nozzle 38, one end of which is fixedly arranged on the inner side wall of the separation box 11, and the lower surface of the scraper frame 36 is in close contact with the upper end surface of the liquid-filling tube 15. When in use, when the liquid-filling tube 15 rotates to the bottom of the scraper frame 36, one end of the top column 18 is in close contact with the upper end of the second guide plate 37, so that the push rod 17 pushes the upper end of the isolation filter plate 16 to be in close contact with the bottom end of the scraper frame 36. As the liquid-filling tube 15 continues to rotate, the scraper frame 36 scrapes the lymphatic tissue on the upper end of the isolation filter plate 16, so that the lymphatic tissue falls into the collection box 43. Example 2

[0028] The working method of the frozen animal lymphatic tissue liquid separation device described in Example 1 comprises the following steps: S1. Cut the softened lymphatic tissue into small-volume coarse materials, then open the first box door and place the small-volume coarse materials in the decomposition tube 23, then control the heating components in the support tube 24 to start through the external control component, and the decomposition net and the decomposition tube 23 are both made of heat-conducting materials, so that the heating components heat the decomposition net and the decomposition tube 23, but the temperature will not damage the cells inside the lymphatic tissue, and at the same time add decomposition liquid to the liquid-filled tube 15. After the small-volume coarse materials in the decomposition tube 23 are thawed, start the electric push rod 25, and the output end of the electric push rod 25 drives the decomposition tube 23 to move, so that the decomposition tube 23 is immersed in the decomposition liquid in the liquid-filled tube 15. The decomposition liquid is placed in the liquid, so that the decomposition liquid performs preliminary decomposition on the lymphatic tissue, and at the same time, the second motor 28 is started, and the second motor 28 drives the first friction wheel 27 to rotate through the first belt. Since the first friction wheel 27 is in close contact with the friction ring 21, the first friction wheel 27 drives the liquid-filling tube 15 to rotate at a low speed through the friction ring 21. After the preliminary decomposition is completed, the output end of the electric push rod 25 drives the decomposition tube 23 to rise, so that the small volume of coarse material inside the decomposition tube 23 is in close contact with the bottom end of the fixed decomposition plate 26. Under the action of the electric push rod 25 and the decomposition net, the small volume of coarse material is crushed into fine lymphatic tissue, and the fine lymphatic tissue falls on the upper end of the isolation filter plate 16; S2, the output end of the first motor 13 drives the liquid-filling tube 15 to rotate 90 degrees for the first time, so that the liquid-filling tube 15 rotates to the bottom of the first sealing plate 30, and the upper end of the liquid-filling tube 15 is in close contact with the bottom end of the first sealing plate 30, and the friction ring 21 and the second friction wheel 29 are in close contact with each other, and then when the second friction wheel 29 rotates, the friction ring 21 drives the liquid-filling tube 15 to rotate at a high speed, so as to accelerate the separation of the fine lymphatic tissue by the decomposition liquid; S3, the first motor 13 drives the liquid-loading tube 15 to rotate 90 degrees for the second time, so that the liquid-loading tube 15 rotates to the bottom end of the second sealing plate 35. In this process, the correction friction ring 22 first contacts the correction plate 31. When the arc surface on the correction friction ring 22 contacts the correction plate 31, the correction friction ring 22 is rotated by the liquid-loading tube 15, and the correction friction ring 22 and the correction plate 31 are tightly pressed against each other, so that the correction friction ring 22 rotates. When one end of the flat notch on the correction friction ring 22 is pressed against one side of the correction plate 31, under the action of a plurality of spring telescopic rods 32, the two rollers at the flat notch are pressed against the correction plate 31, so that the correction friction ring 22 no longer rotates. At the same time, the liquid outlet at the bottom end of the liquid-loading tube 15 is aligned with the liquid collecting tube 34. When the top column 18 is in close contact with the inclined surface of the first guide plate 33, when the installation shaft 12 drives the liquid-filling tube 15 to rotate, one end of the top column 18 moves on the inclined surface of the first guide plate 33, so that the top column 18 pushes the isolation filter plate 16 to move through the push rod 17, and the isolation filter plate 16 pushes the refined lymphatic tissue to move. When one end of the top column 18 is in close contact with the uppermost end of the first guide plate 33, under the action of the upper end of the isolation filter plate 16 and the bottom end of the second sealing plate 35, the refined lymphatic tissue is squeezed, so that the residual liquid inside the refined lymphatic tissue is separated, and at the same time, the sealing rod 20 is separated from the liquid outlet hole, and the decomposition liquid inside the liquid-filling tube 15 is discharged into the liquid collecting tube 34 through the liquid outlet hole, and the liquid collecting tube 34 discharges the decomposition liquid through the drain pipe, which is convenient for subsequent experimental research; S4, the first motor 13 drives the liquid-filling tube 15 to rotate 90 degrees for the third time, so that the liquid-filling tube 15 rotates to the bottom of the cleaning nozzle 38. In this process, the bottom end of the top column 18 first clings to the inclined surface of the second guide plate 37. Under the guidance of the second guide plate 37, the top column 18 pushes the isolation filter plate 16 to slide inside the liquid-filling tube 15 through the push rod 17. When one end of the top column 18 clings to the upper end of the second guide plate 37, the isolation filter plate 16 and one end of the liquid-filling tube 15 are in the same plane, and the scraper frame 36 scrapes the lymphatic tissue on the upper end of the isolation filter plate 16, so that the lymphatic tissue falls into the collection box 43. Inside, at the same time, the friction plate 42 and the third friction wheel 41 are rubbed tightly, so that the third friction wheel 41 drives the ball to rotate 90 degrees, so that the ball valve 40 is in a conducting state, and then water is supplied to the inside of the water inlet pipe 39 through the external water supply component, and the water inlet pipe 39 transports the water source to the inside of the cleaning nozzle 38, and the cleaning nozzle 38 sprays water through a plurality of spray holes, so that the isolation filter plate 16 and the inside of the liquid-filling pipe 15 are cleaned; the first motor 13 drives the liquid-filling pipe 15 to rotate 90 degrees for the fourth time, so that the cleaned liquid-filling pipe 15 rotates to the bottom of the decomposition tube 23, so as to carry out the next separation experiment.

[0029] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A frozen animal lymphatic tissue liquid separation device, characterized in that: The invention comprises a separation box (11), wherein a mounting shaft (12) is provided at the top end of the separation box (11) via a first motor (13), wherein the first motor (13) drives the mounting shaft (12) to rotate, wherein the middle portion of the mounting shaft (12) is rotatably connected to a liquid-filling pipe (15) via a fixing plate (14), wherein a through hole and a liquid outlet are provided at the bottom of the liquid-filling pipe (15), wherein a push rod (17) is provided in the through hole, wherein one end of the push rod (17) extends to the inside of the liquid-filling pipe (15) and is connected to the bottom of an isolation filter plate (16), wherein a sealing A sealing rod (20), one end of which extends to the inside of the liquid-filling tube (15) and is connected to the bottom of the isolation filter plate (16); a decomposition component, a centrifugal component, a liquid separation component and a cleaning component are sequentially arranged inside the separation box (11) along the rotation direction of the fixed plate (14); the decomposition component is used to thaw frozen lymphatic tissue; the centrifugal component is used to drive the liquid-filling tube (15) to rotate; the liquid separation component is used to separate liquid in the liquid-filling tube (15); and the cleaning component is used to clean the liquid-filling tube (15) and the isolation filter plate (16).

2. A frozen animal lymphatic tissue liquid separation device according to claim 1, characterized in that: The decomposition component comprises a decomposition tube (23), the bottom of the decomposition tube (23) is a decomposition net, the bottom of the decomposition net is connected to the output end of the electric push rod (25) through a support tube (24) penetrating the decomposition tube (23), a heating component is provided inside the support tube (24), and the heating component is electrically connected to an external control component, the electric push rod (25) is arranged at the top end inside the separation box (11), a mounting tube is provided at the output end of the electric push rod (25), a fixed decomposition plate (26) is provided at the bottom end of the mounting tube, the outer edge of the fixed decomposition plate (26) is slidably connected to the inner wall of the decomposition tube (23), the upper end of the fixed decomposition plate (26) is connected to the top end of the separation box (11) through a plurality of support rods, a first box door is provided on the side wall of the separation box (11) located at the decomposition tube (23), and a rotating assembly for driving the liquid-filled tube (15) to rotate is provided inside the separation box (11).

3. A frozen animal lymphatic tissue liquid separation device according to claim 2, characterized in that: The rotating assembly comprises a friction ring (21), the friction ring (21) being sleeved on the outer wall of the liquid-containing tube (15); a first friction wheel (27) is provided inside the separation box (11); the first friction wheel (27) is frictionally arranged in close contact with the friction ring (21); the first friction wheel (27) is arranged at one end of a first mounting shaft; a plurality of first rotating plates are rotatably arranged on the first mounting shaft; the first rotating plates are fixedly arranged inside the separation box (11); a first driven wheel is provided at the other end of the first mounting shaft; the first driven wheel is connected to the first driving wheel via a first belt; the first driving wheel is fixedly arranged on the output end of a second motor (28); and the second motor (28) is fixedly arranged at the bottom end inside the separation box (11).

4. A frozen animal lymphatic tissue liquid separation device according to claim 1, characterized in that: The centrifugal component comprises a second friction wheel (29), the second friction wheel (29) being fixedly mounted at one end of a second mounting shaft, a plurality of second rotating plates being rotatably mounted on the second mounting shaft, the second rotating plates being fixedly mounted inside the separation box (11), a second driven wheel being arranged at the other end of the second mounting shaft, the second driven wheel being transmission-connected to a second driving wheel via a second belt, the second driving wheel being fixedly mounted on an output end of a second motor (28), a first sealing plate (30) being arranged above the second friction wheel (29) at a position corresponding to the position of the liquid-filling tube (15), the first sealing plate (30) being fixedly mounted on an inner side wall of the separation box (11), the lower surface of the first sealing plate (30) being in close contact with the upper end of the liquid-filling tube (15).

5. The frozen animal lymphatic tissue liquid separation device according to claim 1, characterized in that: The liquid separation component comprises a first guide plate (33), the first guide plate (33) being arranged at the bottom end of the separation box (11) and corresponding to the other end of the push rod (17), a top column (18) being arranged between the push rod (17) and the first guide plate, the top column (18) being connected to the push rod (17) via a return spring (19), a liquid collecting pipe (34) being arranged on one side of the first guide plate (33) at a position corresponding to the position of the liquid outlet, the liquid collecting pipe (34) being arranged on the side wall of the separation box (11), a liquid discharge pipe being arranged at the bottom end of the liquid collecting pipe (34), one end of the liquid discharge pipe extending to the outside of the separation box (11), a correction mechanism being arranged at a position corresponding to the first guide plate (33) inside the separation box (11), the correction mechanism being used to correct the position of the liquid filling pipe (15).

6. A frozen animal lymphatic tissue liquid separation device according to claim 5, characterized in that: The correction mechanism comprises a correction friction ring (22) and a correction plate (31); the correction plate (31) is arranged above one end of a first guide plate (33) and connected to the inner wall of the separation box (11) via a plurality of spring telescopic rods (32); a correction friction ring (22) is fixedly arranged on the outer wall of the liquid-filling tube (15) at a position corresponding to the correction plate (31); a plane notch is arranged on the correction friction ring (22); rollers are arranged at both ends of the plane notch; an arcuate surface of the correction friction ring (22) is frictionally closely arranged with the correction plate (31); a second sealing plate (35) is arranged above the first guide plate (33) at a position corresponding to the position of the liquid-filling tube (15); a bottom end of the second sealing plate (35) is at the same height as an upper end of the liquid-filling tube (15); and the second sealing plate (35) is fixedly arranged on the inner wall of the separation box (11).

7. A frozen animal lymphatic tissue liquid separation device according to claim 5, characterized in that: The cleaning component comprises a cleaning nozzle (38), the cleaning nozzle (38) being fixedly arranged inside the separation box (11), a plurality of spray holes being arranged at the bottom end of the cleaning nozzle (38), a water inlet of the cleaning nozzle (38) being connected to a water outlet of an external water supply device through a water inlet pipe (39), a control component being arranged at the connection between the water outlet of the water inlet pipe (39) and the water inlet of the cleaning nozzle (38), the control component being used to control the switch of the water inlet of the cleaning nozzle (38), a second guide plate (37) being arranged below the cleaning nozzle (38), the second guide plate (37) being arranged at the bottom end inside the separation box (11) and being located directly below the top column (18), a collection box (43) being arranged between the second guide plate (37) and the bottom of the separation box (11), a filter screen being arranged inside the collection box (43).

8. A frozen animal lymphatic tissue liquid separation device according to claim 7, characterized in that: The control assembly comprises a ball valve (40), the water inlet of the ball valve (40) being connected to the water outlet of the water inlet pipe (39), the water outlet of the ball valve (40) being communicated with the water inlet of the cleaning nozzle (38), the ball valve (40) being fixedly arranged at the top end of the separation box (11) via a support frame, a third friction wheel (41) being arranged at one end of a valve stem of the ball valve (40), a friction plate (42) being frictionally attached to the third friction wheel (41), and the friction plate (42) being fixedly arranged on the upper surface of the fixed plate (14).

9. The frozen animal lymphatic tissue liquid separation device according to claim 1, characterized in that: A scraper frame (36) is provided below the cleaning nozzle (38), one end of the scraper frame (36) is fixedly arranged on the inner wall of the separation box (11), and the lower surface of the scraper frame (36) is in close contact with the upper end surface of the liquid filling pipe (15).

10. A method for operating the frozen animal lymphatic tissue liquid separation device according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1, placing a small volume of coarse material in the decomposition tube (23), adding decomposition liquid into the liquid-filling tube (15), starting the electric push rod (25), immersing the decomposition tube (23) in the decomposition liquid in the liquid-filling tube (15), turning on the heating component in the support tube (24), allowing the decomposition liquid to complete the preliminary decomposition of the lymphatic tissue, starting the second motor (28) to drive the liquid-filling tube (15) to rotate at a low speed, and after the preliminary decomposition is completed, the electric push rod (25) drives the decomposition tube (23) to rise, and the electric push rod (25) and the decomposition net crush the small volume of coarse material into fine lymphatic tissue that falls onto the isolation filter plate (16); S2, the first motor (13) drives the liquid-filling tube (15) to rotate 90 degrees for the first time, the liquid-filling tube (15) rotates to below the first sealing plate (30) and is in close contact with the bottom end of the first sealing plate (30), and the second friction wheel (29) drives the liquid-filling tube (15) to rotate at a high speed via the friction ring (21); S3, the first motor (13) drives the liquid-filling tube (15) to rotate 90 degrees for the second time, the two rollers at the notch of the plane are in close contact with the correction plate (31), the liquid outlet hole is aligned with the liquid collecting tube (34), the isolation filter plate (16) pushes the refined lymphatic tissue to move, and under the action of the isolation filter plate (16) and the second sealing plate (35), the refined lymphatic tissue is squeezed, and the decomposition liquid in the liquid-filling tube (15) is discharged into the liquid collecting tube (34) through the liquid outlet hole, and the liquid collecting tube (34) discharges the decomposition liquid through the discharge pipe; S4, the first motor (13) drives the liquid-filling tube (15) to rotate 90 degrees for the third time, the bottom end of the top column (18) is in close contact with the top end of the second guide plate (37), the scraper frame (36) scrapes the lymphatic tissue on the top end of the isolation filter plate (16), the friction plate (42) and the third friction wheel (41) are in close contact with each other, so that the third friction wheel (41) drives the ball to rotate (90) degrees, the external water supply device supplies water to the inside of the water inlet pipe (39), and the cleaning nozzle (38) sprays water through the spray hole to clean the isolation filter plate (16) and the inside of the liquid-filling tube (15).

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