A method and device for identifying the sensitivity of citrus canker varieties

Abstract

The present invention relates to the technical field of plant pathology, and specifically to a method and device for identifying the sensitivity of citrus canker varieties, comprising a box body and a top plate installed on the box body: an injection assembly for injecting leaves in the method for identifying the sensitivity of citrus canker varieties, the injection assembly is arranged inside the box body, and the injection assembly is used to inject pathogens into citrus leaves; a conveying assembly, the conveying assembly is arranged above the injection assembly, and the conveying assembly is used to convey citrus leaves into the injection position of the injection assembly; a mounting frame, the mounting frame is arranged at one end of the injection assembly, a conveyor belt is arranged above the mounting frame, and a driving motor is arranged on one side of the conveyor belt. Compared with the prior art, the present application realizes smooth transportation after injection by providing a conveying assembly to cooperate with the injection assembly, and ensures the stability and efficiency of the injection operation through the automatic control and precise positioning of the conveying assembly, avoiding errors that may be caused by manual intervention.

Description

Technical Field

[0001] The invention relates to the technical field of plant pathology, and in particular to a method and a device for identifying the sensitivity of citrus canker varieties. Background Art

[0002] Identifying the sensitivity of citrus varieties to ulcer disease is to improve the quality and yield of citrus production. Citrus canker disease is a bacterial disease that seriously threatens the citrus planting industry. It can cause ulcer spots on leaves, fruits, and branches. In severe cases, it can cause early leaf drop, early fruit shedding, and tree decline, which directly affects the yield and quality of citrus. By identifying the sensitivity of different varieties to ulcer disease, it can help select varieties with stronger disease resistance for promotion and planting, thereby reducing losses caused by the disease.

[0003] In the prior art, inoculation is usually carried out on leaves during identification, and the injection inoculation method used is to inoculate 50ul of a bacterial suspension with an OD600 of 0.15 near the midrib on the front side of the unfolded light green, immature leaves. This traditional injection method usually relies on manual operation, which leads to human errors during leaf transportation and injection, affecting the accuracy and efficiency of the injection. For example, when manually aligning the leaves and injecting, it is easy to cause incomplete injection or accidental injury, which will have a negative impact on the experimental results, and after the injection, it needs to be taken away and stored, which is inefficient. Therefore, the present application discloses a method and device for identifying the sensitivity of citrus canker varieties to meet the stability and efficiency of the injection operation. Summary of the invention

[0004] In view of this, the purpose of the present invention is to propose a method and device for identifying the sensitivity of citrus canker varieties, so as to solve the problem that human errors are easily generated during the leaf transportation and injection process, affecting the accuracy and efficiency of injection.

[0005] Based on the above purpose, the present invention provides a method for identifying the sensitivity of citrus canker varieties, comprising the following steps:

[0006] S1: Select healthy and well-growing young citrus leaves, ensure that the leaves are of uniform maturity, prepare pathogen strains and prepare a bacterial suspension of appropriate concentration;

[0007] S2: Clean the young citrus leaves to ensure they are free of contamination, cut the leaves, sterilize their surfaces, and place them in a sterile culture dish for later use;

[0008] S3: Inoculate a certain amount of pathogen suspension onto the back or front surface of the leaf by injection or acupuncture;

[0009] S4: placing the inoculated leaves in a culture environment with controlled temperature and humidity, with the appropriate culture temperature being 25°C to 28°C and the relative humidity being maintained above 80% to promote the growth of the pathogen and the manifestation of symptoms;

[0010] S5: 7 to 10 days after inoculation, regularly observe the formation of lesions on the leaves, quantitatively evaluate the sensitivity of different varieties by measuring the size, number and distribution of lesions, and calculate the lesion area and disease index;

[0011] S6: Based on the expansion and area of ​​leaf lesions, combined with the performance of the variety, a comprehensive analysis is conducted to evaluate the variety's sensitivity to citrus canker. The sensitivity is divided into highly sensitive, moderately sensitive and disease-resistant levels.

[0012] Preferably, the disease index calculation formula is: DI = 100 × ∑ (xi × ni) / 5 × n, wherein: DI - disease index; xi - disease level; ni ​​- number of lesions at the corresponding disease level; i - each level of disease classification; n - total number of lesions investigated.

[0013] The present application also discloses a device for identifying the sensitivity of citrus canker varieties, which is applied in the above-mentioned method for identifying the sensitivity of citrus canker varieties, and comprises:

[0014] A box and a top plate mounted on the box:

[0015] An injection component for injecting the leaves in the method for identifying the sensitivity of citrus canker varieties, wherein the injection component is arranged inside the box and is used for injecting pathogens into the citrus leaves;

[0016] A conveying assembly, the conveying assembly is arranged above the injection assembly, and the conveying assembly is used to convey the citrus leaves into the injection position of the injection assembly;

[0017] A mounting frame is arranged at one end of the injection assembly, a conveyor belt is arranged above the mounting frame, and a driving motor is arranged on one side of the conveyor belt.

[0018] Preferably, the injection assembly includes a mounting plate fixedly mounted inside the box body, a vertical plate is vertically mounted on one side of the top of the mounting plate, a fixed block is arranged on the top of the vertical plate, a quantitative water pump is arranged on the other side of the top surface of the mounting plate, one end of the quantitative water pump is connected to a liquid inlet pipe, the liquid inlet pipe passes through the box body, a hose is arranged at the outlet of the quantitative water pump, a cylinder is arranged on one side of the vertical plate, a push plate is fixedly connected to the telescopic end of the cylinder, a plurality of injection needles are also equidistantly arranged on the top of the fixed block, and a plurality of the injection needles pass through the push plate and the top plate, and a plurality of the push plates are connected to the hose.

[0019] Preferably, a spare storage cylinder is arranged on a side of the vertical plate away from the quantitative water pump, and a connecting pipe is arranged on the top of the spare storage cylinder to communicate with one side of the quantitative water pump.

[0020] Preferably, the conveying assembly includes two groups of fixed plates fixedly installed on one side of the top of the box body and one side of the top of the mounting frame, each group of the fixed plates is provided with two fixed plates, and the fixed plates are arranged in a T shape, a screw rod is rotatably installed between the bottoms of the two groups of fixed plates, and a first slider is slidably installed on the two screw rods, one of the first sliders is threadedly connected to one of the screw rods, and a rotating motor is fixedly installed on one side of the fixed plate where the screw rod is located, and the output end of the rotating motor is fixedly connected to the fixed plate, and two slide rails are respectively arranged between the middle parts of the two groups of fixed plates, and a second slider is slidably installed above each two slide rails, and a placement plate is fixedly connected between the two second sliders, a placement groove is provided in the middle part of the placement plate, and a receiving groove is provided on the bottom surface of the middle part of the placement plate, and two movable plates are rotatably installed inside the receiving groove, a rotating shaft is provided on one side of the two movable plates, and a gap for injection is provided between the two movable plates, and a plurality of second return springs are provided on both sides of the interior of the receiving groove, and the other sides of the plurality of second return springs are respectively fixedly connected to the outer sides of the two movable plates.

[0021] Preferably, a positioning plate is fixedly installed between a group of the fixed plates located on the mounting frame, and the positioning plate is arranged below the slide rail. Several groups of positioning blocks are fixedly installed below the two movable plates, and each group of the positioning blocks is provided with two, and each group of the positioning blocks is arranged in an eight-shaped shape. The side of the positioning plate close to the positioning block is provided with an arc-shaped push block, and a drop hole is opened in the middle of the positioning plate corresponding to the placement groove.

[0022] Preferably, a plurality of telescopic rods are fixedly mounted on the tops of the two first sliding blocks, the telescopic ends of the plurality of telescopic rods are respectively fixedly connected to the bottom ends of the second sliding blocks, and the telescopic ends of the plurality of telescopic rods are sleeved with first return springs.

[0023] Preferably, one side of the slide rail is arranged in a wave shape, and the other side is arranged in a straight line, and a section of the wave-shaped side of the slide rail located above the plurality of syringes is arranged horizontally.

[0024] Preferably, L-shaped plates are respectively provided on both sides of the top surface of the box body, and the two L-shaped plates are arranged opposite to each other. Rotating rollers are rotatably installed on both sides of the opposite surfaces of the two L-shaped plates, and the two rotating roller sleeves are provided with rubber tracks. A tensioning roller is also rotatably installed below the middle of the opposite surfaces of the two L-shaped plates, and a pressure sensor is arranged inside the tensioning roller. Two arc-shaped protrusions are also respectively provided in the middle of the top surface of the placement plate for use with the pressure sensor.

[0025] Beneficial effects of the present invention:

[0026] 1. The method and device for identifying the sensitivity of citrus canker varieties are provided with a conveying component for use in conjunction with an injection component. The conveying component ensures that the citrus leaves can be smoothly moved to the designated position of the injection component through the linkage structure of the screw rod, the slider, the slide rail and the telescopic rod, and the wavy design on one side of the slide rail allows the placement plate to be flexibly adjusted on an uneven track. When the placement plate moves above the injection component, the rubber track provides an additional limiting effect, and the stop position of the blade is accurately controlled by the pressure sensor. When the extrusion force reaches the set value, the conveying component will stop immediately to ensure that the blade is in the best position during injection. Secondly, the conveying component can not only automatically adjust the position of the blade, but also perfectly cooperate with the injection component so that the injection needle can accurately penetrate the blade to perform the injection operation of the pathogen suspension. After the injection is completed, the conveying component will trigger the motor to restart through the cylinder signal, and smoothly move the blade to the subsequent processing area to achieve smooth transportation after injection. Through the automatic control and precise positioning of the conveying component, the stability and efficiency of the injection operation are ensured, and the errors that may be caused by manual intervention are avoided.

[0027] 2. The method and device for identifying the sensitivity of citrus canker varieties are provided with a movable plate and a positioning plate in the conveying assembly. When the placement plate continues to slide and the leaves are conveyed to the top of the rear conveyor belt, the positioning blocks under the two movable plates first contact the push blocks on the positioning plates. The push blocks gradually push the movable plates into the storage grooves during the sliding of the leaves. As the force of the push blocks increases, the movable plates move inward and the placement grooves gradually open. At this time, the leaves automatically fall from the placement grooves and enter the conveyor belt below through the drop holes. After receiving the leaves, the conveyor belt transports them to the storage and observation area at the rear. During the whole process, the leaves are smoothly transferred and the transfer process after injection is automatically completed, thereby realizing the automated processing and transportation of the leaves. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only for the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0029] Figure 1 This is a schematic diagram of the three-dimensional structure of the present invention from a first viewing angle;

[0030] Figure 2 It is a schematic diagram of the three-dimensional structure of the present invention from a second viewing angle;

[0031] Figure 3 It is a schematic diagram of the structure of the box body and the conveying assembly of the present invention;

[0032] Figure 4 This is a schematic structural diagram of the injection assembly of the present invention from the first perspective;

[0033] Figure 5 This is a schematic structural diagram of the injection assembly of the present invention from a second viewing angle;

[0034] Figure 6 It is a schematic diagram of the isometric structure of the conveying assembly of the present invention;

[0035] Figure 7 This is a schematic structural diagram of the conveying assembly of the present invention from a second viewing angle;

[0036] Figure 8 It is a schematic diagram of the planar structure of the conveying assembly of the present invention;

[0037] Fig. 9 It is a schematic diagram of the structure of the movable plate and the positioning plate of the present invention;

[0038] Fig.10 It is a schematic diagram of the working process of the present invention.

[0039] The markings in the figure are:

[0040] 1. Box body; 2. Top plate; 3. Liquid inlet pipe; 4. Mounting plate; 5. Dosing water pump; 6. Vertical plate; 7. Fixed block; 8. Push plate; 9. Cylinder; 10. Injection needle; 11. Hose; 12. Spare storage cylinder; 13. Mounting frame; 14. Conveyor belt; 15. Driving motor; 16. Fixed plate; 17. Screw rod; 18. Slide rail; 19. First slider; 20. Telescopic rod; 21. First return spring; 22. Second slider; 23. Placement plate; 24. Placement slot; 25. Movable plate; 26. L-shaped plate; 27. Rotating roller; 28. Rubber track; 29. ​​Storage slot; 30. Rotating shaft; 31. Positioning block; 32. Positioning plate; 33. Push block; 34. Drop hole; 35. Second return spring. DETAILED DESCRIPTION

[0041] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments.

[0042] It should be noted that, unless otherwise defined, the technical terms or scientific terms used in the present invention should be understood by people with ordinary skills in the field to which the present invention belongs. The "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different components. "Include" or "comprise" and similar words mean that the elements or objects appearing before the word include the elements or objects listed after the word and their equivalents, without excluding other elements or objects. "Connect" or "connected" and similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0043] like Fig.10 A method for identifying the sensitivity of citrus canker varieties comprises the following steps:

[0044] S1: Select healthy and well-growing young citrus leaves, ensure that the leaves are of uniform maturity, prepare pathogen strains and prepare a bacterial suspension of appropriate concentration;

[0045] S2: Clean the young citrus leaves to ensure they are free of contamination, cut the leaves, sterilize their surfaces, and place them in a sterile culture dish for later use;

[0046] S3: Inoculate a certain amount of pathogen suspension onto the back or front surface of the leaf by injection or acupuncture;

[0047] S4: placing the inoculated leaves in a culture environment with controlled temperature and humidity, with the appropriate culture temperature being 25°C to 28°C and the relative humidity being maintained above 80% to promote the growth of the pathogen and the manifestation of symptoms;

[0048] S5: 7 to 10 days after inoculation, regularly observe the formation of lesions on the leaves, quantitatively evaluate the sensitivity of different varieties by measuring the size, number and distribution of lesions, and calculate the lesion area and disease index;

[0049] S6: Based on the expansion and area of ​​leaf lesions, combined with the performance of the varieties, a comprehensive analysis was conducted to evaluate the sensitivity of the varieties to citrus canker. The sensitivity was divided into highly sensitive, moderately sensitive and resistant levels;

[0050] The reference method is as follows:

[0051] Diameter grading standard of lesions

[0052]

[0053] Compared with the existing technology, by selecting healthy and well-growing young citrus leaves and ensuring their uniform maturity, the sample differences in the experiment can be reduced and the consistency of the results can be ensured. Secondly, the leaves are washed and sterilized and then placed in a sterile culture dish. This treatment can effectively avoid the interference of external contamination on the experiment and ensure the accuracy of the inoculated pathogens. During the inoculation process, the injection method or acupuncture method can accurately control the amount and position of the pathogen inoculation, avoiding errors that may be caused by manual operation. By regularly observing the formation of lesions and quantifying their size, number and distribution, this method provides a more intuitive and accurate sensitivity assessment. Finally, a comprehensive analysis combined with the lesion area and variety performance can more scientifically divide the varieties into highly sensitive, moderately sensitive and disease-resistant grades, providing a more accurate basis for disease resistance screening.

[0054] The disease index calculation formula is: DI = 100 × ∑ (xi × ni) / 5 × n, where: DI - disease index; xi - disease level; ni ​​- number of lesions at the corresponding disease level; i - each level of disease classification; n - total number of lesions investigated;

[0055] The reference method is as follows:

[0056] Disease resistance classification standards

[0057] level Disease resistance classification DI range Disease resistance classification explained 0 Immunity (I) DI=0 Plants are completely immune to citrus canker and show no signs of infection 1 High resistance (HR) DI<10 The plant is highly disease resistant and has a very low infection rate 3 Disease resistance (R) 10≤DI<20 Plants are disease resistant and have a low infection rate 5 Disease resistance (T) 20≤DI<40 The plant has a certain tolerance to the disease, and the infection rate is moderate 7 Sickness(S) 40≤DI<60 Plants are susceptible to diseases and have a high infection rate 9 High Sensitivity (HS) DI≥60 The plants are highly susceptible to citrus canker and the infection rate is very high

[0058] Through the calculation formula of the disease index (DI), the sensitivity of different citrus varieties to canker disease can be scientifically and quantitatively evaluated. By introducing the disease level (xi), the corresponding number of lesions (ni) and the total number of lesions (n), this method can more carefully reflect the disease development of the plant. In addition, the different levels in the disease resistance grading standard (such as immune, high resistance, disease tolerance, susceptible, etc.) provide a clear reference, which is convenient for researchers to quickly evaluate the disease resistance of plants based on the DI value, thereby realizing variety screening and optimizing breeding strategies. This standard not only takes into account the disease development speed of the plant, but also refines each disease level to make the experimental results more intuitive and comparable, which helps to distinguish the differences in disease resistance of different varieties. In particular, by setting levels such as "immune, high resistance, disease resistance", plants with extremely high disease resistance can be more accurately distinguished, guiding subsequent variety improvement and cultivation plans, and ultimately improving production efficiency and economic benefits.

[0059] like Figures 1 to 9As shown, the present application also discloses a device for identifying the sensitivity of citrus canker varieties, which is used in the above-mentioned method for identifying the sensitivity of citrus canker varieties, comprising: a box body 1 and a top plate 2 installed on the box body 1; an injection assembly for injecting leaves in the method for identifying the sensitivity of citrus canker varieties, the injection assembly is arranged inside the box body 1, and the injection assembly is used to inject pathogens into citrus leaves; a conveying assembly, the conveying assembly is arranged above the injection assembly, and the conveying assembly is used to convey citrus leaves into the injection position of the injection assembly; a mounting frame 13, the mounting frame 13 is arranged at one end of the injection assembly, a conveyor belt 14 is arranged above the mounting frame 13, and a driving motor 15 is arranged on one side of the conveyor belt 14;

[0060] First, the citrus leaves are transported to the injection position of the injection component through the conveying component, and the position of the leaves is controlled so that they enter the designated injection area. Then, the injection component is started, and the pathogen suspension is injected into the citrus leaves according to the predetermined injection volume and angle to ensure that the pathogens are evenly distributed on the surface or back of the leaves. After the injection is completed, the leaves are continued to be transported to the rear conveyor belt 14 through the conveyor belt 14 to the subsequent observation area. The whole process realizes automated injection and conveying operations, ensuring the efficient and accurate conduct of the experiment.

[0061] like Figures 2 to 5 As shown, the injection assembly includes a mounting plate 4 fixedly mounted inside the box body 1, a vertical plate 6 is vertically mounted on one side of the top of the mounting plate 4, a fixing block 7 is arranged on the top of the vertical plate 6, a quantitative water pump 5 is arranged on the other side of the top surface of the mounting plate 4, one end of the quantitative water pump 5 is connected with a liquid inlet pipe 3, the liquid inlet pipe 3 is arranged through the box body 1, a hose 11 is arranged at the outlet of the quantitative water pump 5, a cylinder 9 is arranged on one side of the vertical plate 6, a push plate 8 is fixedly connected to the telescopic end of the cylinder 9, a plurality of injection needles 10 are also equidistantly arranged on the top of the fixing block 7, and the plurality of injection needles 10 are arranged through the push plate 8 and the top plate 2, and the plurality of push plates 8 are connected to the hose 11, a spare storage cylinder 12 is arranged on the side of the vertical plate 6 away from the quantitative water pump 5, and a connecting pipe is arranged on the top of the spare storage cylinder 12 to communicate with one side of the quantitative water pump 5;

[0062] After the conveying component transports the citrus leaves to the designated injection position, the conveying component stops running. First, the quantitative water pump 5 draws the pathogen suspension supplied from the outside from the liquid inlet pipe 3, and transports the suspension to the injection needle 10 through the hose 11. At the same time, the cylinder 9 drives the push plate 8 to move downward, and the push plate 8 drives several injection needles 10 to penetrate the surface or back of the citrus leaves to complete the injection operation of the bacteria. After the injection is completed, the cylinder 9 retracts the push plate 8, and the injection needle 10 is pulled out of the leaf. The leaf is then moved to the subsequent processing area through the conveying component. The entire injection process is completed automatically, and the provision of the spare storage cylinder 12 further ensures the reserve amount of the pathogen suspension, ensures the continuity of the injection operation, and can replenish the liquid in time when it is insufficient to avoid interruption of the experiment, ensure that the liquid can be smoothly transported, and improve the efficiency and stability of the operation.

[0063] like Figure 3 , Figure 6 , Figure 7 , Figure 8 , Fig. 9 As shown, the conveying assembly includes two groups of fixed plates 16 fixedly installed on one side of the top of the box body 1 and one side of the top of the mounting frame 13, each group of fixed plates 16 is provided with two, and the fixed plates 16 are all arranged in a T shape, and a screw rod 17 is rotatably installed between the bottoms of the two groups of fixed plates 16, and a first slider 19 is slidably installed on the two screw rods 17, one of the first sliders 19 is threadedly connected to one of the screw rods 17, and a rotating motor is fixedly installed on one side of the fixed plate 16 where the screw rod 17 is located, and the output end of the rotating motor is fixedly connected to the fixed plate 16, and two slide rails 18 are respectively arranged between the middle parts of the two groups of fixed plates 16, and a second slider 22 is slidably installed above each of the two slide rails 18, and the two second sliders 22 are fixed between them. A placement plate 23 is fixedly connected, a placement groove 24 is provided in the middle of the placement plate 23, a receiving groove 29 is provided on the bottom surface of the middle of the placement plate 23, two movable plates 25 are rotatably installed inside the receiving groove 29, a rotating shaft 30 is provided on one side of the two movable plates 25, a gap for injection is provided between the two movable plates 25, a plurality of second return springs 35 are provided on both sides of the inside of the receiving groove 29, the other sides of the plurality of second return springs 35 are respectively fixedly connected to the outer sides of the two movable plates 25, a plurality of telescopic rods 20 are fixedly installed on the tops of the two first sliders 19, the telescopic ends of the plurality of telescopic rods 20 are respectively fixedly connected to the bottom ends of the second sliders 22, and the telescopic ends of the plurality of telescopic rods 20 are sleeved with first return springs 21;

[0064] First, the motor drives the screw rod 17 to rotate, driving the first slider 19 to slide along the screw rod 17. At the same time, through the cooperation of the telescopic rod 20 and the first return spring 21, when the slider slides, the telescopic rod 20 extends or shortens, driving the second slider 22 to slide on the slide rail 18, and then the second slider 22 can adjust itself following the fluctuation of the slide rail 18, so that the placement plate 23 moves to the specified position, and the citrus leaves are placed in the placement groove 24 of the placement plate 23. After the conveying assembly is started, the leaves slide to the top of the injection assembly through the slider driven by the screw rod 17, and the injection needle 10 of the injection assembly penetrates through the gap between the two movable plates 25 to inject the leaves;

[0065] One side of the slide rail 18 is arranged in a wave shape, and the other side is arranged in a straight line. The wave-shaped side of the slide rail 18 and a section located above the plurality of syringes is arranged horizontally. L-shaped plates 26 are also arranged on both sides of the top surface of the box body 1. The two L-shaped plates 26 are arranged opposite to each other. Rotating rollers 27 are rotatably installed on both sides of the opposite surfaces of the two L-shaped plates 26. The two rotating rollers 27 are sleeved with rubber tracks 28. A tensioning roller is also rotatably installed below the middle of the opposite surfaces of the two L-shaped plates 26. A pressure sensor is arranged inside the tensioning roller. Two arc-shaped protrusions are also respectively arranged in the middle of the top surface of the placement plate 23 for use with the pressure sensor.

[0066] When the conveying assembly is started, the placement plate 23 slides along the slide rail 18 to the uphill section of the injection position and is squeezed by the rubber track 28. When the pressure sensor senses that the extrusion force of the blade reaches the set value, it immediately sends a signal to the control end to control the rotation motor to stop running. The placement plate 23 stops accurately above the syringe to inject the bacterial suspension. After the injection is completed, the cylinder 9 starts to reset. The signal during the reset process is transmitted to the control end again. The control end starts the rotation motor, and the placement plate 23 continues to slide to transport the blade to the rear conveyor belt 14, completing the entire injection and transportation process.

[0067] A positioning plate 32 is also fixedly installed between a group of fixed plates 16 of the mounting frame 13. The positioning plate 32 is arranged below the slide rail 18. A plurality of groups of positioning blocks 31 are fixedly installed below the two movable plates 25. Each group of positioning blocks 31 is provided with two, and each group of positioning blocks 31 is arranged in an eight-shaped shape. A side of the positioning plate 32 close to the positioning block 31 is provided with an arc-shaped push block 33. A drop hole 34 is opened in the middle of the positioning plate 32 to correspond to the placement slot 24.

[0068] When the placement plate 23 continues to slide and the blades are transported to the top of the rear conveyor belt 14, the positioning blocks 31 under the two movable plates 25 first contact the push blocks 33 on the positioning plates 32. The push blocks 33 gradually push the movable plates 25 into the storage grooves 29 during the sliding of the blades. As the force of the push blocks 33 increases, the movable plates 25 move inward, and the placement grooves 24 gradually open. At this time, the blades automatically fall from the placement grooves 24, pass through the drop holes 34 and enter the conveyor belt 14 below. After receiving the blades, the conveyor belt 14 transports them to the storage and observation area at the rear. During the whole process, the blades are smoothly transferred, and the transfer process after injection is automatically completed, realizing the automated processing and transportation of the blades.

[0069] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention (including the claims) is limited to these examples. Under the concept of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0070] The present invention is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for identifying the sensitivity of citrus canker varieties, characterized in that: The following steps are involved: S1: Select healthy and well-growing young citrus leaves, ensure that the leaves are of uniform maturity, prepare pathogen strains and prepare a bacterial suspension of appropriate concentration; S2: Clean the young citrus leaves to ensure they are free of contamination, cut the leaves, sterilize their surfaces, and place them in a sterile culture dish for later use; S3: Inoculate a certain amount of pathogen suspension onto the back or front surface of the leaf by injection or acupuncture; S4: placing the inoculated leaves in a culture environment with controlled temperature and humidity, with the appropriate culture temperature being 25°C to 28°C and the relative humidity being maintained above 80% to promote the growth of the pathogen and the manifestation of symptoms; S5: 7 to 10 days after inoculation, regularly observe the formation of lesions on the leaves, quantitatively evaluate the sensitivity of different varieties by measuring the size, number and distribution of lesions, and calculate the lesion area and disease index; S6: Based on the expansion and area of ​​leaf lesions, combined with the performance of the variety, a comprehensive analysis is conducted to evaluate the variety's sensitivity to citrus canker. The sensitivity is divided into highly sensitive, moderately sensitive and disease-resistant levels.

2. The method for identifying the sensitivity of citrus canker varieties according to claim 1, characterized in that: The disease index calculation formula is: DI = 100 × ∑ (xi × ni) / 5 × n, where: DI - disease index; xi - disease level; ni ​​- the number of lesions at the corresponding disease level; i - each level of disease classification; n - the total number of lesions investigated.

3. A device for identifying the sensitivity of citrus canker varieties, used in the method for identifying the sensitivity of citrus canker varieties as claimed in claim 1, characterized in that: include: A box body (1) and a top plate (2) mounted on the box body (1): An injection component for injecting the leaves in the method for identifying the sensitivity of citrus canker varieties, wherein the injection component is arranged inside the box (1) and is used to inject pathogens into the citrus leaves; A conveying assembly, the conveying assembly is arranged above the injection assembly, and the conveying assembly is used to convey the citrus leaves into the injection position of the injection assembly; A mounting frame (13) is arranged at one end of the injection assembly, a conveyor belt (14) is arranged above the mounting frame (13), and a driving motor (15) is arranged on one side of the conveyor belt (14).

4. The device for identifying the sensitivity of citrus canker varieties according to claim 3, characterized in that: The injection assembly comprises a mounting plate (4) fixedly mounted inside the housing (1), a vertical plate (6) being vertically mounted on one side of the top of the mounting plate (4), a fixing block (7) being arranged at the top of the vertical plate (6), a quantitative water pump (5) being arranged on the other side of the top surface of the mounting plate (4), one end of the quantitative water pump (5) being connected to a liquid inlet pipe (3), the liquid inlet pipe (3) being arranged through the housing (1), a hose (11) being arranged at the outlet of the quantitative water pump (5), a cylinder (9) being arranged on one side of the vertical plate (6), a push plate (8) being fixedly connected to the telescopic end of the cylinder (9), a plurality of injection needles (10) being equidistantly arranged at the top of the fixing block (7), and the plurality of injection needles (10) being arranged through the push plate (8) and the top plate (2), and the plurality of push plates (8) being connected to the hose (11).

5. The device for identifying the sensitivity of citrus canker varieties according to claim 4, characterized in that: A spare storage cylinder (12) is arranged on a side of the vertical plate (6) away from the quantitative water pump (5), and a connecting pipe is arranged on the top of the spare storage cylinder (12) and is connected to one side of the quantitative water pump (5).

6. The device for identifying the sensitivity of citrus canker varieties according to claim 3, characterized in that: The conveying assembly comprises two groups of fixed plates (16) fixedly mounted on one side of the top end of the box body (1) and one side of the top end of the mounting frame (13), each group of the fixed plates (16) is provided with two, the fixed plates (16) are arranged in a T-shape, a screw rod (17) is rotatably mounted between the bottoms of the two groups of fixed plates (16), a first slider (19) is slidably mounted on the two screw rods (17), one of the first sliders (19) is threadedly connected to one of the screw rods (17), and a rotating motor is fixedly mounted on one side of the fixed plate (16) where the screw rod (17) is located, the output end of the rotating motor is fixedly connected to the fixed plate (16), and two sliding blocks are respectively arranged between the middle parts of the two groups of fixed plates (16). A rail (18), a second slider (22) is slidably installed above each two of the slide rails (18), a placement plate (23) is fixedly connected between the two second sliders (22), a placement groove (24) is opened in the middle of the placement plate (23), a storage groove (29) is opened in the middle bottom surface of the placement plate (23), two movable plates (25) are rotatably installed inside the storage groove (29), a rotating shaft (30) is set on one side of the two movable plates (25), a gap for injection is set between the two movable plates (25), and a plurality of second return springs (35) are set on both sides of the inside of the storage groove (29), and the other sides of the plurality of second return springs (35) are fixedly connected to the outer sides of the two movable plates (25) respectively.

7. The device for identifying the sensitivity of citrus canker varieties according to claim 6, characterized in that: A positioning plate (32) is fixedly installed between a group of the fixed plates (16) located on the mounting frame (13). The positioning plate (32) is arranged below the slide rail (18). A plurality of groups of positioning blocks (31) are fixedly installed below the two movable plates (25). Each group of the positioning blocks (31) is provided with two, and each group of the positioning blocks (31) is arranged in an eight-shaped shape. A side of the positioning plate (32) close to the positioning block (31) is provided with an arc-shaped push block (33). A drop hole (34) is provided in the middle of the positioning plate (32) and corresponds to the placement groove (24).

8. The device for identifying the sensitivity of citrus canker varieties according to claim 7, characterized in that: A plurality of telescopic rods (20) are fixedly mounted on the top of the two first sliding blocks (19), the telescopic ends of the plurality of telescopic rods (20) are respectively fixedly connected to the bottom ends of the second sliding blocks (22), and the telescopic ends of the plurality of telescopic rods (20) are sleeved with first return springs (21).

9. The device for identifying the sensitivity of citrus canker varieties according to claim 8, characterized in that: One side of the slide rail (18) is arranged in a wave shape, and the other side is arranged in a straight line. The wave-shaped side of the slide rail (18) and a section located above the plurality of syringes is arranged horizontally.

10. The device for identifying the sensitivity of citrus canker varieties according to claim 9, characterized in that: L-shaped plates (26) are also provided on both sides of the top surface of the box body (1), and the two L-shaped plates (26) are arranged opposite to each other. Rotating rollers (27) are rotatably installed on both sides of the opposite surfaces of the two L-shaped plates (26), and the two rotating rollers (27) are sleeved with rubber tracks (28). A tensioning roller is also rotatably installed below the middle of the opposite surfaces of the two L-shaped plates (26), and a pressure sensor is arranged inside the tensioning roller. Two arc-shaped protrusions are also respectively provided in the middle of the top surface of the placement plate (23) for use with the pressure sensor.

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