Capsule for regulating gastrointestinal health and preparation method thereof
By using modified chitosan, xylitol compounds and other materials and specific bacteria in healthy capsules, the shortcomings of existing capsules in acid resistance, enteric and shelf life are solved, and higher acid resistance, enteric and storage stability are achieved.
Patent Information
- Application Number
- CN202411975214.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-31
- Publication Date
- 2025-04-11
AI Technical Summary
Existing health capsules are poor in acid resistance, enteric and shelf life, and are unstable in storage in wet weather.
Capsules are made by specific preparation methods using wall materials containing sodium alginate, glycerol, modified chitosan, denaturing proteins and xylitol compounds, combined with core materials of bacteria such as Lactobacillus reuteri and Bifidobacterium longan.
The number of live bacteria in the capsule in the acidic environment is increased, enteric-coating is enhanced, and the number of live bacteria does not decrease when stored in a humid environment for a long time, significantly improving storage stability.
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Abstract
Description
Technical Field
[0001] The invention belongs to a health food, and in particular relates to a capsule for regulating gastrointestinal health and a preparation method thereof. Background Art
[0002] In the fast-paced modern society, people's lifestyles and eating habits have undergone tremendous changes. As a result, the irregular life pattern has made gastrointestinal problems a problem for many people. As an important digestive organ of the human body, the health of the stomach and intestines directly affects the quality of our daily life. Bad eating habits, excessive stress, irregular life and other factors may lead to gastrointestinal diseases. Therefore, it is particularly important to prevent gastrointestinal diseases and maintain gastrointestinal health. What followed was the emergence of a large number of health products and services on the market, aimed at improving people's gastrointestinal conditions, among which "health capsules" are popular because of their convenience.
[0003] In order to improve the digestive system and regulate the stomach, the ingredients of the existing "healthy capsules" mainly include bacteria, dietary fiber or digestive enzymes. Bacteria can help balance the microbial environment in the intestines, promote the growth of beneficial bacteria, and inhibit the activity of harmful bacteria; dietary fiber can increase stool volume, promote intestinal peristalsis, and help defecation; the addition of digestive enzymes assists in decomposing fats, proteins, etc. in food, and reduces the burden on the stomach. Healthy capsules can effectively improve the function of the digestive system and relieve gastrointestinal discomfort caused by improper diet. However, most of the "healthy capsules" on the market currently have poor acid resistance, enteric solubility, and shelf life, and most capsule shells are easily softened by water, which is not conducive to storage in humid weather. In order to overcome the above shortcomings, how to provide a capsule that is acid-resistant, enteric-soluble, and storage-resistant, and can regulate gastrointestinal health even in humid weather, and a preparation method is a problem to be solved by the present invention. Summary of the invention
[0004] The object of the present invention is to provide a capsule for regulating gastrointestinal health and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.
[0005] To achieve the above-mentioned object, the present invention provides the following technical solutions: On the one hand, the present invention provides a capsule for regulating gastrointestinal health, the capsule for regulating gastrointestinal health comprises a core material and a wall material, the core material comprises the following components in parts by weight: bacterial cells;
[0006] The wall material comprises the following components: sodium alginate, glycerol, modified chitosan, denatured protein and xylitol compounds.
[0007] As a further improvement, the bacteria is at least one of Lactobacillus reuteri, Bifidobacterium longum, and Lactobacillus bulgaricus.
[0008] As a further improvement, the preparation method of modified chitosan comprises the following steps:
[0009] (1) placing the mixed chitosan and acid solution into a reactor in an inert environment, and then adding an organic acid and hydrogen peroxide to fully react;
[0010] (2) In an inert environment, continue to add ferulic acid to the substance after the reaction in step (1) and allow the reaction to fully proceed, then perform dialysis and freeze-drying to obtain modified chitosan.
[0011] As a further improvement, the mass ratio of chitosan to ferulic acid is 1:0.3-0.5.
[0012] As a further improvement, the xylitol compound is prepared by reacting xylitol and lauric acid.
[0013] As a further improvement, the method for preparing xylitol compounds comprises the following steps:
[0014] Dissolve xylitol and lauric acid in anhydrous solvent, preheat, and then add lipase and After the molecular sieve, the reaction is fully carried out, and after the reaction is completed, the xylitol compound is purified to obtain.
[0015] As a further improvement, the mass ratio of xylitol to lauric acid is 1.2-3:1.
[0016] As a further improvement, the molecular weight of chitosan is 100,000-300,000.
[0017] As a further improvement, the bacteria is at least one of Bifidobacterium longum and Lactobacillus bulgaricus.
[0018] On the other hand, the present invention also provides a method for preparing a capsule for regulating gastrointestinal health, comprising the following steps:
[0019] (1) Add sodium alginate to deionized water, stir evenly, let stand at low temperature for a period of time, gradually heat to room temperature, and set aside;
[0020] (2) mixing the substance obtained in step (1), denatured protein, xylitol compound, modified chitosan and glycerol to obtain a mixed solution;
[0021] (3) Under sterile conditions, the bacteria are added to the mixture in step (2), mixed evenly, and then solidified, filtered, washed, and dried to obtain capsules for regulating gastrointestinal health.
[0022] Compared with the prior art, the beneficial effects of the present invention are as follows: the capsules for regulating gastrointestinal health prepared by the present invention have a high number of viable bacteria in an acidic environment during a gastric acid resistance test, and have good acid resistance; and when the capsules for regulating gastrointestinal health prepared are stored for a long time in a humid environment, the number of viable bacteria does not decrease or decreases only slightly, and the storage stability is good, and the capsules also have good enteric solubility. DETAILED DESCRIPTION
[0023] The present invention will be described below in conjunction with specific embodiments. It should be noted that the following embodiments are examples of the present invention and are only used to illustrate the present invention, but not to limit the present invention. Other combinations and various modifications within the concept of the present invention may be performed without departing from the spirit or scope of the present invention.
[0024] In the following examples, except for denatured protein A, modified chitosan A, xylitol compound A, and bacterial A, the other compound monomers and related reagents used can be purchased from the market, among which chitosan was purchased from Anhui Zhuolu Biotechnology Co., Ltd.; lipase is lipase Novozym 435, purchased from Novozymes China Biotechnology Co., Ltd.; Molecular sieves were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. with the item number M165304; whey protein was purchased from Changzhou Chuhe Biotechnology Co., Ltd. with the item number ch-66; xylitol was purchased from Henan Pinnuoxi Bioengineering Co., Ltd.; Bifidobacterium longum BBMN68 was isolated from the feces of centenarians in Bama County and preserved in the Culture Collection Center of the Institute of Microbiology, Chinese Academy of Sciences with the collection number CGMCC2265.
[0025] Among them, the room temperature involved in the specific implementation methods of this application is 25°C.
[0026] The method for preparing denatured protein A comprises the following steps:
[0027] Whey protein was added into deionized water, and stirred with a magnetic stirrer at room temperature for 2 hours, and then placed in a 4°C environment for 20 hours. Then, the solution was placed in room temperature for 4 hours, and the pH was adjusted to 8 with a sodium hydroxide aqueous solution to obtain a 6 wt% whey protein solution. The whey protein solution was then heated in a water bath for 0.5 hours at a temperature of 80°C to obtain denatured protein A.
[0028] The preparation method of modified chitosan A comprises the following steps:
[0029] (1) 15 g of chitosan and 1.5 L of 1% glacial acetic acid solution were mixed and placed in a nitrogen inert environment. After excluding the air, 0.026 g of ascorbic acid and 10 g of hydrogen peroxide were added and reacted for 1 h.
[0030] (2) In an inert environment, 6 g of ferulic acid was added to the substance after the reaction in step (1) and the reaction was continued for 20 h. The substance was then placed in a 12000-14000 Da dialysis bag for dialysis. During dialysis, the water was changed every 12 h. After dialysis for 72 h, the substance was freeze-dried to obtain modified chitosan A.
[0031] The preparation method of xylitol compound A comprises the following steps:
[0032] 15.2g of xylitol and 10g of lauric acid were dissolved in 0.85L of tert-butyl alcohol, and preheated in a constant temperature water bath at 50°C with shaking at a speed of 350r / min for 30min. Then 6.68g of lipase and 46g of After the molecular sieve, the reaction was fully carried out. After the reaction was completed, the unreacted xylitol and lauric acid were removed, the solvent was removed by rotary evaporation, and the mixture was dried in vacuo (drying temperature was 40° C.) to obtain xylitol compound A.
[0033] Preparation method of bacterial cell A: In a clean bench, 0.1 mL of frozen Bifidobacterium longum BBMN68 bacterial solution was inoculated into 15 mL of MRS liquid culture medium, and anaerobically cultured for 15 hours in an environment with a temperature of 37° C. and a relative air humidity of 50%. After repeated subculturing for 3 times, activated Bifidobacterium longum was obtained;
[0034] The activated Bifidobacterium longum was inoculated into 300 mL of MRS liquid culture medium at a volume ratio of 0.1%, cultured in an environment of 37°C and 50% relative air humidity for 20 hours, and then centrifuged (centrifugal speed of 4000 rpm, centrifugal time of 20 minutes) to obtain bacterial cells. The bacterial cells were washed with 0.9% saline and centrifuged (centrifugal speed of 4000 rpm, centrifugal time of 20 minutes). After repeating the washing and centrifugation three times, bacterial cell A was obtained.
[0035] The configuration of MRS liquid culture medium is as follows: dissolve 10g peptone, 10g beef extract, 5g yeast extract, 2g dipotassium hydrogen phosphate, 2g diammonium citrate, 5g sodium acetate, 20g glucose, 1mL Tween 80, 0.5g magnesium sulfate, 0.25g manganese sulfate, and 15g agar powder in 1L distilled water, adjust the pH value to 6.2, and sterilize at 121°C for 15min.
[0036] The preparation method of the capsule for regulating gastrointestinal health of Example 1 comprises the following steps:
[0037] (1) Sodium alginate was added to deionized water, stirred evenly at room temperature, placed in a 4°C environment for 16 hours, and then placed at room temperature for another 4 hours to obtain a 5 wt% sodium alginate solution for later use;
[0038] (2) taking 20 g of the 5 wt% sodium alginate solution prepared in step (1), 25 g of denatured protein A, 1 g of xylitol compound A, 2.5 g of modified chitosan A, and 15 g of glycerol, and mixing them by magnetic stirring to obtain a mixed solution, sterilizing the mixed solution in a boiling water bath at 100° C. for 1 h, and using it as a wall material for later use;
[0039] (3) Under sterile conditions, all the prepared bacterial cells A are added to the wall material prepared in step (2), and slowly stirred until the mixture is uniformly mixed. While stirring, 2 mL of the uniformly mixed liquid is drawn with a sterile syringe, and then vertically dropped into a calcium chloride coagulation bath to solidify for 0.5 h, and then filtered with a sterile sieve, washed three times with 0.9% saline, and then placed in a vacuum freeze dryer for freeze drying for 36 h to obtain capsules for regulating gastrointestinal health.
[0040] The preparation method of the capsule for regulating gastrointestinal health of Example 2 comprises the following steps:
[0041] (1) Sodium alginate was added to deionized water, stirred evenly at room temperature, placed in a 4°C environment for 16 hours, and then placed at room temperature for another 4 hours to obtain a 6 wt% sodium alginate solution for later use;
[0042] (2) 15 g of the 6 wt % sodium alginate solution prepared in step (1), 22 g of denatured protein A, 1.2 g of xylitol compound A, 2 g of modified chitosan A, and 16 g of glycerol were mixed by magnetic stirring to obtain a mixed solution, which was sterilized in a boiling water bath at 100° C. for 1 h and used as a wall material for later use;
[0043] (3) Under sterile conditions, all the prepared bacterial cells A are added to the wall material prepared in step (2), and slowly stirred until the mixture is uniformly mixed. While stirring, 2 mL of the uniformly mixed liquid is drawn with a sterile syringe, and then vertically dropped into a calcium chloride coagulation bath to solidify for 0.5 h, and then filtered with a sterile sieve, washed three times with 0.9% saline, and then placed in a vacuum freeze dryer for freeze drying for 36 h to obtain capsules for regulating gastrointestinal health.
[0044] The preparation method of the capsule for regulating gastrointestinal health of Example 3 comprises the following steps:
[0045] (1) adding sodium alginate to deionized water, stirring evenly at room temperature, placing in a 4°C environment for 16 hours, and then placing in room temperature for another 4 hours to obtain an 8 wt% sodium alginate solution for later use;
[0046] (2) taking 20 g of the 8 wt % sodium alginate solution prepared in step (1), 30 g of denatured protein A, 1.5 g of xylitol compound A, 3.5 g of modified chitosan A, and 24 g of glycerol, and mixing them by magnetic stirring to obtain a mixed solution, sterilizing the mixed solution in a boiling water bath at 100° C. for 1 h, and using it as a wall material for later use;
[0047] (3) Under sterile conditions, all the prepared bacterial cells A are added to the wall material prepared in step (2), and slowly stirred until the mixture is uniformly mixed. While stirring, 2 mL of the uniformly mixed liquid is drawn with a sterile syringe, and then vertically dropped into a calcium chloride coagulation bath to solidify for 0.5 h, and then filtered with a sterile sieve, washed three times with 0.9% saline, and then placed in a vacuum freeze dryer for freeze drying for 36 h to obtain capsules for regulating gastrointestinal health.
[0048] The preparation method of the capsule for regulating gastrointestinal health of Comparative Example 1 is basically the same as that of Example 1, and the components and contents are basically the same as those of Example 1, except that 3.5 g of modified chitosan A is replaced by 3.5 g of chitosan.
[0049] The preparation method of the capsule for regulating gastrointestinal health of Comparative Example 2 is basically the same as that of Example 1, and the components and contents are basically the same as those of Example 1, except that 3.5 g of modified chitosan A is replaced by 2.5 g of chitosan and 1 g of ferulic acid.
[0050] The preparation method of the capsule for regulating gastrointestinal health in Comparative Example 3 is basically the same as that in Example 1, and the components and contents are basically the same as those in Example 1, except that 1.5 g of xylitol compound A is replaced by 1.5 g of xylitol.
[0051] The preparation method of the capsule for regulating gastrointestinal health of Comparative Example 4 is basically the same as that of Example 1, and the components and contents are basically the same as those of Example 1, except that 1.5g of xylitol compound A is replaced by 1.5g of xylitol, and 3.5g of modified chitosan A is replaced by 3.5g of chitosan.
[0052] The capsules for regulating gastrointestinal health prepared in Examples 1-3 and Comparative Examples 1-4 were subjected to gastric acid resistance test, enteric solubility test and storage stability test, and the determination method was as follows:
[0053] Gastric acid resistance test: 0.5 g of the capsules for regulating gastrointestinal health prepared in Examples 1-3 and Comparative Examples 1-4 were added to 30 mL of simulated gastric fluid (wherein the pH of the simulated gastric fluid was 2.0), and kept warm at 37° C. The number of viable bacteria was determined after being kept warm for 2 h and 4 h, respectively. The specific test method was as follows: after washing with sterile saline to neutrality and dissolving with capsule decomposition fluid, the number of viable bifidobacteria was determined;
[0054] Storage stability test: The gastrointestinal health regulating capsules prepared in Examples 1-3 and Comparative Examples 1-4 were stored at a temperature of 25° C. and a relative humidity of 70%. Samples were taken after 2 months and 4 months of storage to determine the number of viable bacteria.
[0055] The results of the gastric acid resistance test are shown in Table 1, as shown in Table 1 below:
[0056] Table 1
[0057]
[0058] From the comparison between Example 1 and Comparative Examples 1-2 in Table 1, it can be seen that the viable bacterial count of the capsule for regulating gastrointestinal health prepared by using modified chitosan has a high viable bacterial count after being kept warm for 2 hours, and the viable bacterial count does not decrease or decreases insignificantly after being kept warm for 4 hours;
[0059] From the comparison of Example 1 and Comparative Example 3 in Table 1, it can be seen that the number of viable bacteria in the capsules for regulating gastrointestinal health prepared using xylitol compounds still maintains a relatively high number of viable bacteria when kept warm for 2 hours, but the number of viable bacteria after keeping warm for 4 hours does not decrease or decreases only slightly. The use of xylitol compounds improves the gastric acid resistance of the bacteria in the human body for a relatively long time. From the comparison of Example 1 and Comparative Example 4, it can be seen that the number of viable bacteria in the capsules for regulating gastrointestinal health prepared by the combination of modified chitosan and xylitol compounds not only has a high number of viable bacteria after keeping warm for 2 hours, but also does not decrease or decreases only slightly after keeping warm for 4 hours, and a synergistic effect occurs.
[0060] The results of the storability test are shown in Table 2, as shown in Table 2 below:
[0061] Table 2
[0062]
[0063]
[0064] From the comparison between Example 1 and Comparative Examples 1-2 in Table 2, it can be seen that the capsules for regulating gastrointestinal health prepared by using modified chitosan still have a high number of viable bacteria when stored in a humid environment for 2 months, and the number of viable bacteria does not decrease significantly after being stored for 4 months, and the sustained-release effect is obvious. The capsules for regulating gastrointestinal health prepared by the present invention can be stored for a longer time under normal temperature and humid conditions;
[0065] From the comparison of Example 1 and Comparative Example 3 in Table 2, it can be seen that when the capsules for regulating gastrointestinal health prepared by using xylitol compounds are stored in a humid environment for 4 months, the number of live bacteria does not decrease significantly compared with that stored for 2 months. From the comparison of Example 1 and Comparative Example 4, it can be seen that the capsules for regulating gastrointestinal health prepared by using the combination of modified chitosan and xylitol compounds have a high number of live bacteria when stored in a humid environment for 2 months, and the number of live bacteria does not decrease significantly after storage for 4 months. The capsules for regulating gastrointestinal health prepared by the present invention have better storage stability.
[0066] It can be seen from Examples 1-3 that the gastrointestinal health regulating capsules prepared from the components provided by the present invention have a high number of viable bacteria in a gastric acid environment during the gastric acid resistance test. Even when stored in a gastric acid environment for a long time, the number of viable bacteria does not decrease or decreases insignificantly, and the acid resistance is good. The number of viable bacteria in the prepared gastrointestinal health regulating capsules has a high number of viable bacteria when stored in a humid environment for 2 months. Even after storage for 4 months, the number of viable bacteria still does not decrease significantly, and the storage stability is good. At the same time, 0.5 g of the gastrointestinal health regulating capsules prepared in Example 1 was added 30 mL of simulated gastric fluid (wherein the pH of the simulated gastric fluid is 1.2) was placed at 37°C for 3 h, and then NaOH solution was added dropwise to adjust the pH to 7.2. Then 30 mL of simulated intestinal fluid was added and the mixture was shaken and dissolved in a constant temperature shaking incubator (temperature of 37°C, speed of 150 rpm). The absorbance of the intestinal fluid was measured at 600 nm every 10 min within 1 h. The absorbance of Example 1 gradually increased within 0-0.5 h, and the absorbance tended to 2.6 after 0.5 h. The number of viable bacteria at 0.5 h was 10 8 CFU / g, with good enteric solubility.
[0067] The above implementation modes are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with this technology to understand the content of the present invention and implement it, and they cannot be used to limit the protection scope of the present invention. Any equivalent changes or modifications made according to the spirit of the present invention should be included in the protection scope of the present invention.
Claims
1. A capsule for regulating gastrointestinal health, characterized in that: The capsule for regulating gastrointestinal health comprises a core material and a wall material, wherein the core material comprises the following components in parts by weight: bacterial cells; The wall material comprises the following components: sodium alginate, glycerol, modified chitosan, denatured protein and xylitol compounds.
2. A capsule for regulating gastrointestinal health according to claim 1, characterized in that: The bacterial body is at least one of Lactobacillus reuteri, Bifidobacterium longum and Lactobacillus bulgaricus.
3. The capsule for regulating gastrointestinal health according to claim 1, characterized in that: The preparation method of the modified chitosan comprises the following steps: (1) placing the mixed chitosan and acid solution into a reactor in an inert environment, and then adding an organic acid and hydrogen peroxide to fully react; (2) In an inert environment, continue to add ferulic acid to the substance after the reaction in step (1) and allow the reaction to fully proceed, then perform dialysis and freeze-drying to obtain modified chitosan.
4. The capsule for regulating gastrointestinal health according to claim 3, characterized in that: The mass ratio of chitosan to ferulic acid is 1:0.3-0.
5.
5. The capsule for regulating gastrointestinal health according to claim 1, characterized in that: The xylitol compound is prepared by reacting xylitol and lauric acid.
6. The capsule for regulating gastrointestinal health according to claim 1, characterized in that: The method for preparing the xylitol compound comprises the following steps: Dissolve xylitol and lauric acid in anhydrous solvent, preheat, and then add lipase and After the molecular sieve, the reaction is fully carried out, and after the reaction is completed, the xylitol compound is purified to obtain.
7. The capsule for regulating gastrointestinal health according to claim 6, characterized in that: The mass ratio of xylitol to lauric acid is 1.2-3:
1.
8. The capsule for regulating gastrointestinal health according to claim 3, characterized in that: The molecular weight of the chitosan is 100,000-300,000.
9. The capsule for regulating gastrointestinal health and the preparation method thereof according to claim 1, characterized in that: The bacterial body is at least one of Bifidobacterium longum and Lactobacillus bulgaricus.
10. A method for preparing a capsule for regulating gastrointestinal health according to any one of claims 1 to 9, characterized in that: The following steps are involved: (1) Add sodium alginate to deionized water, stir evenly, let stand at low temperature for a period of time, gradually heat to room temperature, and set aside; (2) mixing the substance obtained in step (1), denatured protein, xylitol compound, modified chitosan and glycerol to obtain a mixed solution; (3) Under sterile conditions, the bacteria are added to the mixture in step (2), mixed evenly, and then solidified, filtered, washed, and dried to obtain capsules for regulating gastrointestinal health.