Hard-water-resistant cleansing composition and application thereof in cosmetics

Abstract

The invention discloses a hard-water-resistant face cleaning composition and application thereof in cosmetics, the hard-water-resistant face cleaning composition comprises 0.8-5 parts by weight of lauryl hydroxyl sulfobetaine, 0.1-0.7 part by weight of sodium diethylene triamine pentamethylene phosphonate, 0.045-0.3 part by weight of sodium glucoheptonate, 0.08-1 part by weight of a skin conditioner A and 0.01-0.5 part by weight of a skin conditioner B. The skin conditioner A is 1, 2-propylene glycol, and the skin conditioner B is 1, 2-propylene glycol. The skin conditioning agent A comprises 1, 3-propylene glycol, 1, 2-hexanediol and capryloyl hydroximic acid, and the skin conditioning agent B comprises glycerol and stevia rebaudiana leaf / stem extract. The hard-water-resistant cleansing composition prepared by the invention enhances the hard-water-resistant capacity, reduces the influence of ions on skin, overcomes the obvious defects that commercially available cleansing products are poor in washing feeling in softness and hardness and cannot be effective, can be applied to most of cleansing systems, and can be widely applied to the fields of skin care products, cosmetics and the like.

Description

Technical Field

[0001] The invention belongs to the field of skin care products and cosmetics, and in particular relates to a hard water-resistant facial cleansing composition and application thereof in cosmetics. Background Art

[0002] At present, the focus of cleaning products on the market is gradually shifting to the mildness of the products, but consumers often tend to ignore the possible impact of ions in domestic water on the skin. On the one hand, the water used by most consumers for facial cleansing products is tap water, especially in northern my country, where the water hardness is mostly high. 2+ When it reaches 2.0mmol / L, the proliferation activity of cells decreases rapidly. At this concentration, cells may die or apoptosis due to high calcium load, thus showing a certain cytotoxic effect and affecting the self-renewal metabolism rate of the skin. Urban water can also lead to the adsorption and deposition of heavy metals due to long-distance transportation. For example, the accumulation of excessive iron and copper ions in the skin will enhance the generation of highly active oxygen free radicals, such as hydroxyl free radicals, thereby stimulating oxidative damage and reacting with skin lipid peroxidation to cause inflammation. At the same time, some disinfectants are pre-added to tap water, and the most commonly used disinfectant is chlorinated disinfectant. If the residual chlorine concentration is too high, it will cause damage to skin cells and intercellular matrix, and easily cause dry skin, chapped skin, papules, acne, aging, or palm keratinization. Literature reports that these ions will be deposited on the skin surface, and tap water cannot effectively clean facial dirt. Therefore, the accumulation of these ions and the accumulation of dirt may cause irritation during the cleaning process, making it not gentle to wash your face with "clean water".

[0003] At present, the commonly used surfactants for facial cleansing have poor decontamination ability in hard water. Calcium and magnesium will generate an insoluble calcium soap particle material with these surfactants, which will clog pores, form embolism, affect skin metabolism, and even cause premature skin aging. At the same time, water hardness will also affect the foaming performance of surfactants and the feeling of facial cleansing. A single chelating agent is added to the general facial cleansing formula system as a softening agent for hard water, but simply adding, for example, disodium EDTA cannot effectively ensure the hard water stability of the surfactant in the system, and long-term and large-scale use will irritate the skin. The amount added in the facial cleanser is also not enough to solve and cover the ion-enriched water environment. And the inability to tolerate hard water causes the activity of the surfactant to be reduced in the aqueous solution with a certain hardness, the foaming performance to be reduced, and the feeling of use to be worse. Therefore, the combination of ordinary facial cleansing and hard water may aggravate the harshness of cleaning.

[0004] Chinese patent document CN117297988A discloses an amino acid facial cleanser and a preparation method thereof. In this invention patent, sodium cocoyl glycinate is used as the main component, and after compounding with surfactants potassium cocoyl glycinate, sodium lauroyl glutamate, cocoyl glutamic acid and polyglycerol-10 laurate, the hard water resistance of the prepared facial cleanser can be increased, a large amount of stable and delicate foam is generated, and it has excellent moisturizing and decontamination capabilities. The use conditions of this composition will be limited.

[0005] Chinese patent document CN116999341A discloses a hard water resistant composition and its preparation method and application. The composition in this invention patent only solves the possible impact of calcium and magnesium ions in water on cleaning products, and does not consider the impact of other ions such as iron, copper, and chlorine ions that may exist in water on human skin. At the same time, the main solution in the patent is to use the compounding of surfactants, and the deionization ability is weak. Summary of the invention

[0006] In order to overcome the problems in the prior art, the present invention provides a hard water-resistant cleansing composition and its application in cosmetics, which achieves coverage of various types of ions and more effectively reduces the penetration residue of ions. At the same time, the composition of the present invention is added to a general cleansing system to improve the long-term storage stability of the product in terms of odor, color, performance, etc., and help the system improve its antiseptic and antibacterial capabilities.

[0007] In order to solve the above technical problems, the technical solution proposed by the present invention is: The present invention provides a hard water resistant facial cleansing composition, comprising the following raw materials in parts by weight: lauryl hydroxysulfobetaine, sodium diethylenetriamine pentamethylenephosphonate, sodium glucoheptonic acid, a skin conditioner A and a skin conditioner B, wherein the skin conditioner A is 1,3-propylene glycol, 1,2-hexanediol and caprylhydroxamic acid, and the skin conditioner B is glycerin and stevia leaf / stem extract, and the parts by weight are as follows: Lauryl Hydroxysulfobetaine 0.24-1.5 parts by weight 0.1-0.7 parts by weight of sodium diethylenetriamine pentamethylethylenephosphonate Sodium glucoheptonate 0.045-0.3 parts by weight Skin conditioning agent A 0.08--1 weight part Skin conditioning agent B 0.01-0.5 parts by weight.

[0008] As an optional embodiment, in the facial cleansing composition provided by the present invention, the skin conditioner A comprises, by weight, 2-8 parts of caprylhydroxamic acid, 20-40 parts of 1,2-hexanediol, and 52-78 parts of 1,3-propylene glycol.

[0009] As an optional embodiment, in the facial cleansing composition provided by the present invention, the skin conditioner B comprises, by weight, 49-61 parts of glycerin, 36-46 parts of water, and 3-5 parts of stevia leaf / stem extract.

[0010] As an optional embodiment, in the facial cleansing composition provided by the present invention, the preparation method of the skin conditioning agent B is a subcritical fluid extraction method.

[0011] As an optional embodiment, in the facial cleansing composition provided by the present invention, the preparation method of the skin conditioning agent B comprises the following steps: The stevia leaves / stems are dried, crushed with a pulverizer, filtered through a 60-mesh screen, and then dried and stored for later use. A certain amount of dry powder is weighed and added to a high-temperature and high-pressure reactor at a solid-liquid ratio of 1:1-1:5, stirred evenly, and nitrogen is introduced for exhaust. Subcritical extraction is performed at a pressure of 0.5-3MPa and a temperature of 70-350°C. After the extraction is completed, the subcritical water extract is collected and centrifuged at 3000-8000r / min for 5-15min, the supernatant is evaporated and concentrated, and mixed with glycerol to obtain a sample skin conditioner B.

[0012] As an optional embodiment, the facial cleansing composition provided by the present invention includes the following raw materials in parts by weight: 3% lauryl hydroxysulfobetaine, 0.4% sodium diethylenetriamine pentamethylenephosphonate, 0.15% sodium glucoheptonic acid, 0.5% skin conditioner A and 0.1% skin conditioner B.

[0013] Based on the same technical concept, the present invention also provides the use of the hard water resistant facial cleansing composition in the preparation of cleansing skin care products.

[0014] Based on the same technical concept, the present invention also provides a skin care product, comprising the above-mentioned facial cleansing composition.

[0015] Based on the same technical concept, the present invention also provides the use of the hard water resistant facial cleansing composition in the preparation of cosmetics.

[0016] Based on the same technical concept, the present invention also provides a cosmetic comprising the above-mentioned facial cleansing composition.

[0017] The lauryl hydroxysulfobetaine in the present invention is a hard water resistant surfactant, which has a large hydrophilic group and a straight chain lipophilic group, has a good hard water resistant effect, and has the same foaming effect in soft and hard water. Sodium diethylenetriamine penta methylenephosphonate can be used in a wide pH range, can integrate with free metal ions, make them lose catalytic oxidation activity, and secondly can effectively prevent the oxidation chain reaction and chemical decomposition of substances, inhibit the formation of peroxide complexes, thereby maximally protecting the long-term stability of the final product. Sodium glucoheptonate is a multivalent chelating agent, and its integration ability is 2-3 times that of common chelating agents sodium gluconate and EDTA under the same conditions. When used as an integration agent in a strong alkaline medium, the solubility and stability of sodium gluconate are much better than sodium gluconate EDTA. The active ingredient of STEVIA REBAUDIANA leaf / stem extract is extracted from organic leaves by subcritical water extraction technology. It has a high polyphenol content and can form stable complexes with metal ions on the skin to form an ion protection barrier membrane for the skin, synergizing with other active ingredients. Caprylhydroxamic acid is a commonly used preservative. Because its molecules contain oxygen and nitrogen atoms with lone pairs of electrons, and the oxygen and nitrogen atoms are close to each other, they can chelate with metal ions such as calcium, iron, magnesium, and aluminum to form stable polycyclic metal chelates, which can effectively solve the interference of metal ions to cosmetic formulas and achieve the purpose of stabilizing the quality of the formula.

[0018] The specific analysis is as follows: Lauryl Hydroxysulfobetaine is a lime soap dispersant (LSDA), a hard water resistant surfactant that allows soap to be used as an effective laundry detergent in hard water, rather than forming an insoluble lime soap precipitate. Surfactants used as lime soap dispersants must have a bulky hydrophilic group (such as an ester, amine, or amino group as a linking group between the terminal hydrophilic group and the lipophilic group) and a straight chain lipophilic group. The bulky hydrophilic group of the lime soap dispersant allows the mixed micelles with the hydrophilic groups arranged toward the water phase to remain convexly curved toward the water phase. The micelles formed by the soap molecules themselves are believed to be reversed in hard water, with the lipophilic groups facing the water phase, resulting in insoluble lime soap.

[0019] Sodium diethylenetriamine pentamethylethylenephosphonate can be used in a wide pH range and can integrate with free metal ions to make them lose their catalytic oxidation activity. Secondly, it can effectively prevent the oxidation chain reaction and chemical decomposition of substances and inhibit the formation of peroxide complexes, thereby maximizing the protection of the long-term stability of the final product.

[0020] Sodium glucoheptonate is one of the few natural chelating agents that can perform super strong chelation in strong alkaline conditions, especially when pH is above 11. Sodium glucoheptonate is a starch derivative and a leading bio-based new material in China. It can also be used as an antioxidant to help protect the skin from free radical damage. It can also be used as a moisturizer to enhance the product's ability to retain moisture and keep the skin moisturized.

[0021] In skin conditioner A, 1,2-hexanediol is considered to be one of the mildest diols, harmless to the human body, and has good antibacterial and moisturizing effects. Secondly, octanoyl hydroxamic acid is a commonly used, highly efficient and selective metal ion chelating agent. Its molecules contain oxygen and nitrogen atoms with lone pairs of electrons, and the oxygen and nitrogen atoms are close to each other, which can bind to Ca 2+ , Fe 3+ Mg 2+ 、Al 3+ The metal ions such as octanoyl hydroxamic acid undergo chelation to form a stable polycyclic metal chelate. This is because octanoyl hydroxamic acid can form a unique three-dimensional structure, which can effectively chelate various metal ions, including highly efficient chelated iron ions, which are the nutrients required for mold growth. At the same time, octanoyl hydroxamic acid is more gentle and safe in antiseptic applications because it does not directly attack microbial cells, but only blocks the way for microbial cells to obtain iron ions.

[0022] Skin conditioner B is a transparent liquid containing stevia leaf / stem extract. It has a high content of polyphenols extracted using subcritical water extraction technology and can form stable complexes with metal ions on the skin to form an ion protection barrier membrane for the skin, synergizing with other active ingredients.

[0023] Compared with the prior art, the present invention has the following beneficial effects: In the present invention, sodium diethylenetriamine penta methylene phosphonate, sodium glucoheptonic acid and stevia leaf / stem extract promote each other, can synergize, enhance the chelating ability of ions, solve the potential risk of ions in clean water, and skin conditioner A and lauryl hydroxysulfonyl betaine also have an additive effect on the functional ingredients. Secondly, the above-mentioned functional ingredients can cooperate with skin conditioner A to play an antiseptic and antibacterial role. The last three aspects synergize with each other, and none of them is indispensable, and good effects are exerted. The composition of the present invention as a whole strengthens the ability to resist hard water, reduces the impact of ions on the skin, makes up for the obvious shortcomings of commercially available cleansing products in terms of softness and hardness, and cannot be effective, increases the stability of cleaning products, and at the same time, the composition of the present invention is mild and non-irritating, and after addition, a product development that is milder than "clean water" face washing is achieved, and can be applied to most cleansing systems, and is widely used in the fields of skin care products and cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0025] Figure 1 The test results of the calcium ion chelating ability of the facial cleansing composition in the examples and comparative examples of the present invention; Figure 2 The test results of the iron ion chelating ability of the cleansing composition in the examples and comparative examples of the present invention are shown in FIG. Figure 3 The inhibitory effect of the facial cleansing composition of the present invention on the release of IL-6; Figure 4 It is the result of the effect of the facial cleansing composition of the present invention on human hemoglobin; Figure 5 This is a comparative diagram of the facial cleansing composition in Example 1 of the present invention and the control facial cleanser; Figure 6 The sensory evaluation results of the cleansing composition in Example 1 of the present invention and the control cleansing composition are shown in FIG. DETAILED DESCRIPTION

[0026] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively and meticulously below in conjunction with the accompanying drawings and preferred embodiments of the present invention, but the protection scope of the present invention is not limited to the following specific embodiments.

[0027] Unless otherwise defined, all professional terms used below have the same meanings as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present invention.

[0028] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0029] Example 1 A hard water resistant facial cleansing composition, characterized in that it comprises the following raw materials: lauryl hydroxysulfobetaine, sodium diethylenetriamine pentamethylenephosphonate, sodium glucoheptonic acid, skin conditioner A and skin conditioner B, wherein skin conditioner A is 1,3-propylene glycol, 1,2-hexanediol and caprylhydroxamic acid, and skin conditioner B is glycerol and stevia leaf / stem extract. Wherein skin conditioner A comprises the following components by weight: 5 parts of caprylhydroxamic acid, 30 parts of 1,2-hexanediol, and 65 parts of 1,3-propylene glycol. Skin conditioner B comprises the following components by weight: 55 parts of glycerol, 41 parts of water, and 4 parts of stevia leaf / stem extract. The details are shown in Table 1 below.

[0030] Table 1: Raw material ratios of the facial cleansing compositions in the examples and comparative examples

[0031] The preparation method of skin conditioner B is as follows: dry stevia leaves / stems, crush them with a pulverizer, filter them through a 60-mesh screen, and then dry them for later use. Weigh a certain amount of dry powder, add it to a high-temperature and high-pressure reactor at a solid-liquid ratio of 1:1-1:5, stir it evenly, introduce nitrogen to exhaust, perform subcritical extraction at a pressure of 0.5-3MPa and a temperature of 70-350°C, collect the subcritical water extract after the extraction is completed, and centrifuge it at 3000-8000r / min for 5-15min, take the supernatant, evaporate and concentrate it, and mix it with glycerol to obtain the sample skin conditioner B.

[0032] Example 2-3 The hard water resistant facial cleansing composition in Example 2-3 is different from that in Example 1 mainly in the different ratios of raw materials, as shown in Table 1 above.

[0033] Comparative Examples 1-6 The hard water resistant facial cleansing compositions in Comparative Examples 1-6 are different from those in Example 1 mainly in the different ratios of raw materials, as shown in Table 1 above.

[0034] The effects of the hard water resistant facial cleansing compositions in the examples and comparative examples were verified as follows: (1) Chelating ability test of the composition 1) Calcium ion chelation ability test Test principle: Adjust the pH value of the solution to about 10. The chelating agent has the ability to complex metal calcium ions. When the calcium ions are combined to saturation, the calcium ions combine with oxalate ions to form water-insoluble calcium oxalate precipitates, and the titration reaches the endpoint.

[0035] Experimental methods: Weigh about 5g of the integrator, dissolve it in water and dilute it to a 500mL volumetric flask, draw 10mL, add 5mL of ammonia-ammonium chloride buffer solution to adjust the pH value to about 10, then add 1mL of 3% sodium oxalate solution, and titrate with calcium acetate standard solution until the solution becomes turbid.

[0036] x=(cV100) / (m×10 / 500) In the formula: c is the concentration of calcium acetate standard solution, mol / L; V is the volume of calcium acetate standard solution consumed in titration, mL; m is the mass of chelating agent, g; 100 is the molar mass of CaCO3.

[0037] The chelating ability results are shown in Table 2.

[0038] Table 2: Test results of the chelating ability of the cleansing compositions in the examples and comparative examples for calcium ions

[0039] In Comparative Example 1, a conventional anionic surfactant is used; in Comparative Example 2, the chelating agent combination with synergistic enhancement is replaced with the commonly used EDTA-2Na; in Comparative Example 3, the antiseptic composition skin conditioning agent A is replaced with the commonly used sodium benzoate; in Comparative Examples 4, 5, and 6, the other two aspects are removed, respectively, and the synergistic anti-hard water surfactant, the hard water antiseptic composition, and the plant extract are removed. From Table 2 and Figure 1 It can be seen that the compositions provided by Examples 1-3 of the invention have strong calcium ion chelating ability, and compared with a single chelating agent, the ability is greatly improved. When the main functional component in the composition is replaced, its chelating ability is significantly reduced, and after removing the two aspects of the synergistic surface active and antiseptic composition, it will also decrease to a certain extent. At the same time, by comparing Comparative Example 1 and Comparative Example 4, Comparative Example 3, Comparative Example 5 and Comparative Example 6, it can be seen that when we use ordinary surfactants and ordinary preservatives, there is almost no addition to the ability of the overall efficacy combination.

[0040] 2) Iron ion chelation ability test Experimental method: Accurately weigh 5.000g of sample, add deionized water to dissolve, transfer to a 500mL volumetric flask to the mark, shake well and set aside. Transfer 2mL of sample solution to a 250mL conical flask, add 30mL of water and 0.2g of 2% sulfosalicylic acid, and titrate with 0.01mol / L ammonium ferric sulfate standard solution until the solution changes from colorless to slightly reddish as the end point. The calculation formula is as follows: X=VCx159.6x500 / m×2 Wherein, V is the volume of ammonium ferric sulfate solution consumed by the sample (mL); c is the concentration of ammonium ferric sulfate solution (mol / L); and m is the mass of the sample (g).

[0041] The chelating ability results are shown in Table 3.

[0042] Table 3: Test results of the chelating ability of the cleansing compositions in the examples and comparative examples for iron ions

[0043] From Table 3 and Figure 2 It can be seen that the compositions provided by Examples 1-3 of the present invention also have a strong chelating ability for iron ions. When the main functional component in the composition is replaced, its chelating ability has a significant decrease, and after removing the two aspects of surface activity and preservative or extract with synergistic effect, it will also decrease to a certain extent. At the same time, by comparing Comparative Example 1 and Comparative Example 4, Comparative Example 3, Comparative Example 5 and Comparative Example 6, it can be seen that when we use common surfactants and common preservatives, there is almost no addition to the ability of the overall efficacy combination.

[0044] (2) Chloride removal capacity test Experimental method: simulate the disinfection of tap water, refer to the "Sanitary Standard for Drinking Water" GB5749-2022, and make high-chlorine water. Mix water and the test solution in a ratio of 90:10 for testing, stir for 1 minute, and use a residual chlorine water quality tester to measure the free chlorine content. The test results before and after dechlorination are shown in Table 4 below.

[0045] Table 4: Chlorine removal results of the facial cleansing compositions in the examples and comparative examples

[0046] The test results of chloride ion removal are as above, and the compositions provided in Examples 1-3 of the present invention also have excellent removal effects on chloride ions. At the same time, by comparing Comparative Example 1 with Comparative Example 2, it can be seen that the improvement of the system's chloride removal by sodium diethylenetriamine penta methylene phosphonate, sodium glucoheptonic acid and chrysanthemum leaf / stem extract is also much greater than EDTA-2Na.

[0047] (3) Hard water resistance test Test method: The experiment refers to the "Sanitary Standard for Drinking Water" GB5749-2022 to produce high calcium and magnesium ion water. Take 50ml of the test solution and let it flow down the wall of the cylinder. Mark the liquid level inside the measuring cylinder as the initial scale. Then use a measuring cylinder to take 500ml of the test solution and slowly pour it into the separating funnel so that the metering tube connected to the bottom of the separating funnel is located at the 450mm scale line on the horizontal plane of the 50ml test solution in the internal measuring cylinder. Open the control valve of the separating funnel to allow the test solution to flow down continuously until the liquid level in the separating funnel drops to the 150mm scale line and stops. Close the control valve and measure the foam volume (only foam) in the measuring cylinder 30s after the liquid flow stops. Record the data and take the average value.

[0048] Hard water resistance ratio = (hard water bubble volume / soft water bubble volume) * 100% The results of the hard water resistance test are shown in Table 5 below.

[0049] Table 5: Test results of hard water resistance in Examples and Comparative Examples

[0050] The experimental results show that the main surfactants commonly used in facial cleansing on the market have poor resistance to hard water, and there is a large difference in the foaming ability between soft and hard water. However, the composition of the present invention has good foaming capacity when added to the main surfactant system commonly used in facial cleansing on the market, and there is no obvious difference between soft and hard water, showing excellent resistance to hard water.

[0051] (4) Anti-corrosion ability test Test method: Add Example 1 to the cleansing system of the blank formula to prepare a cleansing product, and compare and verify it with the blank sample. By adding mixed microorganisms to the sample, bacteria are usually mixed together, and fungi (molds, yeasts) are mixed together during the test. This method simulates the characteristics of the mixed coexistence of microorganisms in nature. Refer to the international general standard for cosmetics antiseptic challenges and judge by the logarithmic reduction value of the microbial content on the 2nd / 7th / 14th / 21st / 28th day.

[0052] The control cleanser-blank formula is shown in Table 6 below.

[0053] Table 6: Control Cleanser - Blank Formulation

[0054] The anti-corrosion ability test results are shown in Table 7 below.

[0055] Table 7: Antiseptic results of facial cleansing compositions in Examples and Comparative Examples

[0056] The antiseptic challenge results are as above. It can be seen from Table 7 that the normal amino acid cleansing system cannot pass the antiseptic test when using 1% phenoxyethanol alone. The reduction rate of bacteria and fungi mixtures within 7 days did not reach below 99.9%, but was only about 70%. After adding Example 1, no significant increase of bacteria and molds occurred in the 28-day test, and the surface antiseptic test was passed. The composition of the present invention can assist the normal cleansing system in strengthening antiseptic.

[0057] (5) System stability comparison test Test method: Example 1 was added to a cleansing system of a blank formula to prepare a cleansing product, and the cleansing product was compared and verified with the blank sample. The color change of the cleansing sample under high temperature and light addition was compared and judged. The blank sample was consistent with the formula in the above experiment.

[0058] The results are shown in Table 8 below.

[0059] Table 8: Comparative results of the stability of the facial cleansing composition systems in the examples and comparative examples

[0060] In the facial cleansing system, especially the transparent facial cleansing liquid system, amino acid surfactants or pigments tend to change color under long-term high temperature and light, such as deepening the color, affecting the stability and appearance of the system. Example 1 of the present invention has strong antioxidant and anti-chemical decomposition effects, and can effectively improve the long-term storage stability of the smell, color, performance and other aspects of cleaning products. After 2 hours and 60 days of observation at a high temperature of 48°C in the light box, the appearance of the product without the addition of the composition obviously turned yellow, while the color of the facial cleansing product with the addition of the composition of Example 1 basically did not change. The results are as follows Figure 5 shown.

[0061] (6) Inhibition of macrophage IL-6 action Test method: Cell inoculation: RAW264.7 cells were inoculated in a 96-well plate at 4000 cells / well and cultured in a 37°C, 5% CO2 incubator for 24 hours. The working solution of the test substance was prepared according to the experimental design. When the cell plating rate in the 96-well plate reached 80%-90%, the 10mg / ml LPS solution was diluted to 1μg / ml and added to the plate at 50μL / well. The blank control group was added with an equal amount of culture medium and cultured in a 37°C, 5% CO2 incubator for 4 hours. According to the test plan, group medication was carried out, and finally the IL-6 content in the culture medium was detected using a kit.

[0062] The results are as follows Figure 3 As shown. Compared with the negative group, the IL-6 content in the positive group increased significantly, indicating that the stimulation conditions of this experiment were effective. The results showed that the high-hardness water prepared in the laboratory and the commercially available cleansing + high-hardness water could not inhibit the release of IL-6, while Example 1 of the present invention added to hard water could significantly improve the hard water stimulation and had a significant inhibitory effect on the release of IL-6, showing a mild effect after improving the influence of ions.

[0063] (7) Comparative test of human body cleaning with high hardness water Test method: Recruit 30+ volunteers with normal skin condition, aged between 18-40 years old (except pregnant or lactating women); use high-hardness water configured in the laboratory, and compare the left and right faces, which are the control cleansing face in Table 6 and the control cleansing face with added Example 1. Test the change of clean skin condition, and the test cycle is 4 weeks. Use VISIA skin detector to take photos of the patient's face in the frontal position and bilateral 45° oblique position, collect and obtain control data, and keep the measurement environment (temperature, humidity, irradiation light source) relatively constant.

[0064] The results are as follows Figure 4 As shown. Long-term use of high-hardness water for cleaning will cause minerals to accumulate on the skin and damage the skin barrier over time. After using a control cleanser without chelating ability, the facial hemoglobin area increased significantly, 3 times the initial value. The cleanser after adding Example 1 can remove most of the ions in hard water, and the test results show a lower increase in hemoglobin, showing an improvement effect on the potential risks of hard water and a certain improvement in the mildness of the cleanser.

[0065] (8) Consumer sensory evaluation test Test method: In order to evaluate the improvement effect of the composition involved in the present invention on skin feel such as foam, Example 1 with synergistic enhancement is added to the cleansing system to prepare a cleansing product, which is compared and verified with a blank sample. The hard water-resistant composition is applied to the cleansing system. 30 healthy volunteers were selected for sensory evaluation test. During the facial application process, the texture, foam, cleansing power, rinsing speed, dryness, tightness, mildness and preference are scored and evaluated from multiple dimensions. The score range is 1-9 points (preference is 0-10 points). The higher the score, the higher the degree of recognition of the dimension. The evaluation criteria are shown in Table 9 below.

[0066] Table 9: Sensory evaluation criteria

[0067] The evaluation results are shown in Table 10 below.

[0068] Table 10: Sensory evaluation results of the cleansing compositions in Examples and Comparative Examples

[0069] The facial cleanser prepared in Example 1 of the present invention is superior to the blank facial cleanser on the market in all evaluation indicators (except for cleaning power), and the overall preference of the volunteers is relatively high. Among them, the difference between the foam of Example 1 and the foam of the control facial cleanser in the dimension of foam richness is most obvious. This is because the facial cleanser added in Example 1 can significantly reduce the hardness of domestic water, increase the foam richness and stability, and enhance the washing feeling. Specifically, Figure 6 shown.

[0070] The above contents are further detailed descriptions of the present invention in combination with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the scope of protection of the present invention.

Claims

1. A hard water resistant facial cleansing composition, characterized in that: The invention comprises the following raw materials in parts by weight: Lauryl Hydroxysulfobetaine 0.24-1.5 parts by weight 0.1-0.7 parts by weight of sodium diethylenetriamine pentamethylethylenephosphonate Sodium glucoheptonate 0.045-0.3 parts by weight Skin conditioning agent A 0.08--1 weight part Skin conditioning agent B 0.01-0.5 parts by weight The skin conditioning agent A is 1,3-propylene glycol, 1,2-hexanediol and caprylhydroxamic acid, and the skin conditioning agent B is glycerin and stevia leaf / stem extract.

2. The hard water resistant facial cleansing composition according to claim 1, characterized in that: The skin conditioning agent A comprises, by weight, 2-8 parts of octanoylhydroxamic acid, 20-40 parts of 1,2-hexanediol, and 52-78 parts of 1,3-propylene glycol.

3. The hard water resistant facial cleanser composition according to claim 1, characterized in that: The skin conditioning agent B comprises, by weight, 49-61 parts of glycerin, 36-46 parts of water, and 3-5 parts of stevia leaf / stem extract.

4. The hard water resistant facial cleansing composition according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 3% of lauryl hydroxysulfobetaine, 0.4% of sodium diethylenetriamine pentamethylenephosphonate, 0.15% of sodium glucoheptonic acid, 0.5% of skin conditioning agent A and 0.1% of skin conditioning agent B.

5. The hard water resistant facial cleansing composition according to claim 1, characterized in that: The preparation method of the skin conditioning B is a subcritical fluid extraction method.

6. The hard water resistant facial cleansing composition according to claim 1, characterized in that: The preparation method of the skin conditioning B comprises the following steps: The stevia leaves / stems are dried, crushed with a pulverizer, filtered through a 60-mesh screen and then dried again, the dry powder is weighed, added into a high-temperature and high-pressure reactor at a liquid-to-solid ratio of 1:1-1:5, stirred evenly, and then nitrogen is introduced for exhaust, and subcritical water extraction is performed under the conditions of a pressure of 0.5-3MPa and a temperature of 70-350°C. After the extraction is completed, the subcritical extract is collected and centrifuged at 3000-8000r / min for 5-15min, the supernatant is evaporated and concentrated, and mixed with glycerol to obtain a sample skin conditioner B.

7. Use of the hard water resistant facial cleanser composition according to any one of claims 1 to 6 in the preparation of cleansing skin care products.

8. A skin care product, characterized in that: The cleansing composition comprises the cleansing composition according to any one of claims 1 to 6.

9. Use of the hard water resistant facial cleansing composition according to any one of claims 1 to 6 in the preparation of cosmetics.

10. A cosmetic, characterized in that: The cleansing composition comprises the cleansing composition according to any one of claims 1 to 6.

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