Rotary manual nucleic acid extraction micro-fluidic chip

Abstract

The invention provides a rotary manual nucleic acid extraction micro-fluidic chip, and relates to the technical field of micro-fluidic chips, the rotary manual nucleic acid extraction micro-fluidic chip comprises an upper shell, a lower shell, sealing rubber, a sealing ring, a rotary sliding button, a magnetic bar sleeve and a magnetic bar, the sealing rubber is located on the upper surface of the lower shell, and the upper shell is located on the upper surface of the sealing rubber; the upper surface of the lower shell is provided with a sample adding cavity, a first washing solution, a second washing solution and an eluent, the annular side face of the lower shell is provided with alignment scale marks, high-purity nucleic acid can be effectively extracted through the whole multi-step cracking, washing and elution process, full proceeding of all the steps can be ensured in a manual uniform mixing and magnetic bead adsorption separation mode, and the detection efficiency is greatly improved. According to the kit, the extraction efficiency is improved, the reagent is embedded in the cavity of the lower shell of the kit in advance and is sealed by the sealing rubber, so that the kit is convenient to use, the stability and the safety of the reagent are ensured, the waste and the pollution of the reagent are avoided, and the positions of the cavities can be accurately adjusted according to requirements due to the rotary design.

Description

Technical Field

[0001] The invention belongs to the technical field of microfluidic chips, and more specifically, particularly relates to a rotary manual nucleic acid extraction microfluidic chip. Background Art

[0002] A nucleic acid extraction instrument is a laboratory device used to automatically or semi-automatically separate and purify nucleic acids (including DNA and RNA) in samples. This type of instrument uses matching nucleic acid extraction reagents, combined with mechanical operations and physical and chemical principles, such as centrifugation, magnetic bead adsorption, filter membrane adsorption, elution and other methods, to effectively and efficiently extract pure nucleic acids from various biological samples such as cells, tissues, and body fluids. In the past, nucleic acid extraction required an instrument and an external power supply to complete the operation. However, in some field environments, it is often inconvenient to carry instruments and power supplies. Manual nucleic acid extraction chips can extract nucleic acids without power using consumable chips and simple tools.

[0003] Therefore, in view of this, the existing structure and defects are studied and improved, and a rotary manual nucleic acid extraction microfluidic chip is provided, in order to achieve a more practical purpose. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a rotary manual nucleic acid extraction microfluidic chip to solve the above problems.

[0005] The invention discloses a rotary manual nucleic acid extraction microfluidic chip, comprising an upper shell, a lower shell, a sealing rubber, a sealing ring, a rotating sliding button, a magnetic rod sleeve and a magnetic rod, wherein the sealing rubber is located on the upper surface of the lower shell, the upper shell is located on the upper surface of the sealing rubber, the upper surface of the lower shell is provided with a sample loading chamber, a washing solution 1, a washing solution 2 and an eluting solution, the annular side surface of the lower shell is provided with alignment scale lines, the magnetic rod sleeve is moved upward, and then the lysate cavity is rotated to the position of the sample loading port, the sample and magnetic beads are added into the sample loading chamber, the upper shell is rotated to rotate the lysate cavity to a completely sealed position, the chip is shaken by hand to fully mix the lysate, the sample and the magnetic beads, the upper shell is rotated to rotate the sample loading chamber to the position of the magnetic rod, and then the magnetic rod is inserted, and the sample is left to stand for two minutes to allow the magnetic beads to be adsorbed on the magnetic rod sleeve, and the rotating sliding button is arranged on the annular side surface of the upper shell.

[0006] Preferably, various reagents are pre-buried in the cavity of the lower shell of the reagent box and sealed by a sealing rubber, the sealing ring is movably connected to the magnetic rod sleeve, and a sample addition notch is opened on the surface of the sealing rubber.

[0007] Preferably, the magnetic rod can be inserted into or taken out of the magnetic rod sleeve, so that the magnetic rod sleeve can be magnetic or non-magnetic. The magnetic rod sleeve is movably connected to the lower shell, and the magnetic rod sleeve can be inserted into the hole of the lower shell or taken out from the hole. After the magnetic rod sleeve is inserted downward into the cavity, the cavity can be sealed.

[0008] Preferably, the upper shell and the sealing rubber can rotate relative to each other to achieve the transfer of magnetic beads, and various reagents are pre-embedded in the lower shell. By rotating the upper shell, moving the magnetic rod, moving the rotating sliding button and manually mixing, the magnetic beads can be mixed and transferred in each hole.

[0009] Compared with the prior art, the present invention has the following beneficial effects:

[0010] In the present invention, the magnetic rod can be inserted into or taken out of the magnetic rod sleeve, and the magnetic state of the magnetic rod sleeve can be flexibly controlled. When it is necessary to adsorb magnetic beads, the magnetic rod is inserted to make the magnetic rod sleeve magnetic. When it is necessary to release the magnetic beads, the magnetic rod is taken out and the magnetic rod sleeve loses its magnetism. The magnetic rod sleeve can not only adsorb the magnetic beads for transfer, but also be inserted into the hole of the lower shell to seal the cavity, thereby ensuring that the reagent will not leak during the operation. The design of the relative rotation of the upper shell and the lower shell enables different cavities to correspond to the positions of the magnetic rod in sequence, which is convenient for the transfer of magnetic beads and various operations. The rotation operation of the upper shell is simple and convenient, and the user can accurately adjust the position according to the extraction steps. Various reagents pre-buried in the lower shell provide a necessary chemical environment for nucleic acid extraction. Different reagents play a role in different steps, such as a lysate is used to release nucleic acids from a sample, washing solutions 1 and 2 are used to remove impurities, and an eluent is used to elute nucleic acids from magnetic beads. The entire extraction process does not require large instruments and equipment and can be completed only by manual operation. The chip is small in size, easy to carry, and suitable for on-site detection and emergency situations.

[0011] In the present invention, various required nucleic acid extraction reagents are pre-placed in a specific cavity of the lower shell of the reagent kit and sealed with a sealing rubber, thereby ensuring that the reagents will not leak, evaporate or be contaminated by the outside during storage and transportation, thereby maintaining the stability and effectiveness of the reagents. When performing a test, the magnetic rod cover is first moved upward so that it does not affect subsequent operations, and then the cavity containing the lysate is accurately rotated to the sample injection port position by rotating the upper shell, which is convenient for operation.

[0012] In the present invention, operations such as rotating the upper shell, inserting and removing the magnetic rod, and manual mixing are simple and easy to understand, and can be mastered without professional technical training. The transfer and mixing process of the magnetic beads is fast and efficient, and nucleic acid extraction can be completed in a shorter time. At the same time, manual mixing can ensure that the reagent is in full contact with the sample, thereby improving the extraction efficiency. Users can choose different reagents and operation sequences according to actual needs, and have high flexibility. Compared with traditional nucleic acid extraction equipment, the chip has a lower cost, which reduces the cost of nucleic acid detection.

[0013] In the present invention, the alignment scale lines can provide a clear reference to ensure that each rotation can be accurately positioned, avoiding operational errors caused by inaccurate positioning. A rotating sliding button is provided on the surface of the upper shell, making the action of rotating the upper shell easier and smoother. Compared with directly holding the upper shell by hand for rotation, the button design can better control the rotation force and angle, and improve the accuracy of the operation. The setting of the sample adding chamber provides a specific area for adding samples, making the addition of samples more convenient and centralized.

[0014] In the present invention, when performing detection, the magnetic rod cover is first moved upward so that it does not affect subsequent operations. Then, by rotating the upper shell, the cavity containing the lysis solution is accurately rotated to the sample loading port position, which is convenient for operation. Then, the sample and magnetic beads are added to the sample loading cavity to prepare for the subsequent nucleic acid lysis reaction. By rotating the upper shell, the lysis solution cavity is in a completely sealed state, which can prevent liquid leakage during the mixing process. By shaking the chip by hand, the lysis solution, the sample and the magnetic beads can be fully contacted, thereby promoting the nucleic acid lysis reaction.

[0015] In the present invention, high-purity nucleic acid can be effectively extracted through a multi-step lysis, washing and elution process. Manual mixing and magnetic bead adsorption separation can ensure that each step is fully carried out and improve the extraction efficiency. The reagent is pre-buried in the cavity of the lower shell of the reagent kit and sealed by a sealing rubber, which is convenient for use and ensures the stability and safety of the reagent, avoiding waste and pollution of the reagent.

[0016] In the present invention, the rotary design allows each cavity to accurately adjust its position as needed, facilitating operations of different steps, and can flexibly select different reagents and operation sequences according to actual conditions to meet different nucleic acid extraction needs. The manual nucleic acid extraction chip does not require expensive instruments and equipment, has a low cost, and is suitable for large-scale promotion and application. At the same time, the pre-embedded and sealed design of the reagents also reduces the amount of reagents used and reduces costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0018] Figure 2It is a schematic diagram of the upper shell structure of the present invention;

[0019] Figure 3 It is a schematic diagram of the sealing rubber structure of the present invention;

[0020] Figure 4 It is a schematic diagram of the lower shell structure of the present invention;

[0021] Figure 5 It is a schematic diagram of the overall cross-sectional structure of the present invention;

[0022] Figure 6 It is a side structural schematic diagram of the present invention.

[0023] In the figure, the corresponding relationship between the component names and the figure numbers is: 1, upper shell; 2, lower shell; 3, sealing rubber; 5, magnetic rod; 6, magnetic rod cover; 7, rotating sliding button; 8, sealing ring; 9, sample addition notch; 10, sample addition port; 11, sample addition chamber; 12, washing solution 1; 13, washing solution 2; 14, eluent; 15, alignment scale line. DETAILED DESCRIPTION

[0024] The following is a further detailed description of the embodiments of the present invention in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0025] See also Figure 1 - Figure 6The present invention provides a rotary manual nucleic acid extraction microfluidic chip, comprising an upper shell 1, a lower shell 2, a sealing rubber 3, a sealing ring 8, a rotating sliding button 7, a magnetic rod sleeve 6 and a magnetic rod 5, the sealing rubber 3 is located on the upper surface of the lower shell 2, the upper shell 1 is located on the upper surface of the sealing rubber 3, the upper surface of the lower shell 2 is provided with a sample loading chamber 11, a washing liquid 12, a washing liquid 2 13 and an eluent 14, the annular side of the lower shell 2 is provided with a positioning scale line 15, the magnetic rod sleeve 6 moves up, and then the lysate cavity is rotated to the sample loading port 10 The sample and magnetic beads are added to the sample adding chamber 11, the upper shell 1 is rotated to rotate the lysate chamber to a completely sealed position, the chip is shaken by hand to fully mix the lysate, the sample and the magnetic beads, the upper shell 1 is rotated to rotate the sample adding chamber 11 to the position of the magnetic rod 5, and then the magnetic rod 5 is inserted, and it is left to stand for two minutes to allow the magnetic beads to be adsorbed on the magnetic rod sleeve 6. The rotating sliding button 7 is set on the annular side of the upper shell 1, and the magnetic rod 5 can be inserted or removed from the magnetic rod sleeve 6, and the magnetic state of the magnetic rod sleeve 6 can be flexibly controlled. When the magnetic beads are to be adsorbed, the magnetic rod 5 is inserted to make the magnetic rod sleeve 6 magnetic. When the magnetic beads need to be released, the magnetic rod 5 is taken out and the magnetic rod sleeve 6 loses its magnetism. The magnetic rod sleeve 6 can not only adsorb the magnetic beads for transfer, but also be inserted into the hole of the lower shell 2 to seal the cavity, ensuring that the reagent will not leak during operation. The design of the relative rotation of the upper shell 1 and the lower shell 2 allows different cavities to correspond to the positions of the magnetic rod 5 in turn, which is convenient for the transfer of magnetic beads and various operations. The rotation operation of the upper shell 1 is simple and convenient, and the user can accurately adjust the position according to the extraction steps. The various reagents pre-buried in the lower shell 2 provide the necessary chemical environment for nucleic acid extraction. Different reagents play a role in different steps, such as the lysis solution is used to release the nucleic acid from the sample, the washing solution 12 and the washing solution 2 13 are used to remove impurities, and the elution solution 14 is used to elute the nucleic acid from the magnetic beads. The entire extraction process does not require large-scale instruments and equipment, and can be completed only by manual operation. The chip is small in size and easy to carry, suitable for on-site detection and emergency situations.

[0026] Various reagents are pre-buried in the cavity of the lower shell 2 of the reagent kit and sealed by a sealing rubber 3. The sealing ring 8 is movably connected to the magnetic rod sleeve 6. A sample addition notch 9 is provided on the surface of the sealing rubber 3. The operations of rotating the upper shell 1, inserting and removing the magnetic rod 5, and manually mixing are simple and easy to understand, and can be mastered without professional technical training. The transfer and mixing process of the magnetic beads is fast and efficient, and nucleic acid extraction can be completed in a shorter time. At the same time, manual mixing can ensure that the reagents are in full contact with the samples, thereby improving the extraction efficiency. Users can choose different reagents and operation sequences according to actual needs, and have higher flexibility. Compared with traditional nucleic acid extraction equipment, the chip has lower cost and reduces the cost of nucleic acid detection. The rotary manual nucleic acid extraction chip has broad application prospects in the fields of medical care, public health, food safety, etc., especially in on-site rapid detection, primary medical institutions and areas with limited resources. The chip can provide a convenient and efficient solution for nucleic acid detection. At the same time, with the continuous development and improvement of technology, the chip is expected to be applied in more fields.

[0027] The magnetic rod 5 can be inserted into the magnetic rod sleeve 6 or taken out from the magnetic rod sleeve 6, so that the magnetic rod sleeve 6 can be magnetic or non-magnetic. The magnetic rod sleeve 6 is movably connected to the lower shell 2, and the magnetic rod sleeve 6 can be inserted into the hole of the lower shell 2 or taken out from the hole. After the magnetic rod sleeve 6 is inserted downward into the cavity, the cavity can be sealed, and the various required nucleic acid extraction reagents are pre-placed in the specific cavity of the lower shell 2 of the reagent kit, and sealed with the sealing rubber 3 to ensure that the reagents will not leak, evaporate or be contaminated by the outside world during storage and transportation, and maintain the stability and effectiveness of the reagents. When performing the test, first move the magnetic rod sleeve 6 upward so that it does not affect the subsequent operation. Then, by rotating the upper shell 1, the cavity containing the lysate is accurately rotated to the position of the sample addition port 10. This position The upper shell 1 is convenient for operation, and then the sample and magnetic beads are added to the sample loading chamber 11 to prepare for the subsequent nucleic acid cleavage reaction. The lysate chamber is in a completely sealed state by rotating the upper shell 1, which can prevent liquid leakage during the mixing process. The chip is shaken by hand to make the lysate, sample and magnetic beads fully contact, promote the cleavage reaction of the nucleic acid, release the nucleic acid from the sample and combine with the magnetic beads. The upper shell 1 is rotated to adjust the sample loading chamber 11 to the position of the magnetic rod 5. After inserting the magnetic rod 5, it is allowed to stand for two minutes, and the magnetic properties of the magnetic rod cover 6 are used to adsorb the magnetic beads on the magnetic rod cover 6, so as to separate the magnetic beads from other liquid components. The magnetic rod cover 6 and the magnetic rod 5 are moved upward together to avoid interference with subsequent operations. Then, the upper shell 1 is rotated to adjust the position of the magnetic rod 5 to the position of the magnetic rod 5. The magnetic rod cover 6 is aligned with the cavity where the washing liquid 12 is located, and then, the magnetic rod cover 6 is moved down to allow the magnetic beads to enter the washing liquid 12, and the magnetic rod 5 is taken out. Manual mixing can make the magnetic beads fully cleaned in the washing liquid 12 to remove impurities, and the magnetic rod cover 6 is moved down again, and the magnetic rod 5 is inserted. It is left to stand for two minutes to allow the magnetic beads to be re-adsorbed on the magnetic rod cover 6 to achieve separation of the magnetic beads from the washing liquid 12, and the above operation is repeated in the cavity where the washing liquid 13 is located to wash the magnetic beads for the second time to further improve the purity of the nucleic acid, and the upper shell 1 is rotated to align the position of the magnetic rod 5 with the cavity where the elution liquid 14 is located, and the above operation is repeated to elute the magnetic beads in the elution liquid 14 and enter it. Finally, the magnetic rod cover 6 and the magnetic rod 5 are moved upward together, and the upper shell 1 is rotated to allow the elution liquid 14 to be filled. The cavity moves to the notch position, which is convenient for taking out the eluent 14 into the centrifuge tube for standby use, so as to carry out subsequent nucleic acid detection and analysis. The surface of the lower shell 2 is provided with alignment scale lines 15, which can help the user to accurately rotate the upper shell 1 and the lower shell 2 to a specific position during operation. When the lysis liquid cavity, the eluent 14 cavity, the washing liquid 12 and the washing liquid 2 13 cavity are aligned with the position of the magnetic rod 5, the alignment scale lines 15 can provide a clear reference to ensure that each rotation can be accurately in place to avoid operational errors due to inaccurate position. The surface of the upper shell 1 is provided with a rotating sliding button 7, which makes the action of rotating the upper shell 1 easier and smoother. Compared with directly holding the upper shell 1 by hand for rotation, the button design can better control the strength and angle of rotation.To improve the accuracy of the operation, the setting of the sample adding chamber 11 provides a specific area for adding samples, making the addition of samples more convenient and centralized.

[0028] The upper shell 1 and the sealing rubber 3 rotate relative to each other to realize the transfer of magnetic beads. Various reagents are pre-embedded in the lower shell 2. By rotating the upper shell 1, moving the magnetic rod 5, moving the rotating sliding button 7 and manually mixing, the magnetic beads can be mixed and transferred in each hole. The whole process of multi-step lysis, washing and elution can effectively extract high-purity nucleic acid. The manual mixing and magnetic bead adsorption and separation methods can ensure the full implementation of each step and improve the extraction efficiency. The reagents are pre-embedded in the cavity of the lower shell 2 of the reagent kit and sealed by the sealing rubber 3, which is convenient for use and ensures the stability and safety of the reagents, avoiding waste and pollution of the reagents. The rotary design allows each cavity to accurately adjust its position as needed, which is convenient for operations in different steps. Different reagents and operation sequences can be flexibly selected according to actual conditions to meet different nucleic acid extraction needs. The manual nucleic acid extraction chip does not require expensive instruments and equipment, has low cost, and is suitable for large-scale promotion and application. At the same time, the pre-embedded and sealed design of the reagents also reduces the amount of reagents used and reduces the cost.

[0029] Working principle:

[0030] 1. The magnetic rod 5 can be inserted or taken out of the magnetic rod sleeve 6, and the magnetic state of the magnetic rod sleeve 6 can be flexibly controlled. When it is necessary to adsorb the magnetic beads, the magnetic rod 5 is inserted to make the magnetic rod sleeve 6 magnetic. When it is necessary to release the magnetic beads, the magnetic rod 5 is taken out and the magnetic rod sleeve 6 loses its magnetism. The magnetic rod sleeve 6 can not only adsorb the magnetic beads for transfer, but also be inserted into the hole of the lower shell 2 to seal the cavity, ensuring that the reagent will not leak during operation. The relative rotation design of the upper shell 1 and the lower shell 2 allows different cavities to correspond to the positions of the magnetic rod 5 in turn, which is convenient for the transfer of magnetic beads and various operations. The rotation operation of the upper shell 1 is simple and convenient, and the user can accurately adjust the position according to the extraction steps. The various reagents pre-buried in the lower shell 2 provide the necessary chemical environment for nucleic acid extraction. Different reagents play a role in different steps, such as the lysis solution is used to release the nucleic acid from the sample, the washing solution 12 and the washing solution 2 13 are used to remove impurities, and the elution solution 14 is used to elute the nucleic acid from the magnetic beads. The entire extraction process does not require much The rotary manual nucleic acid extraction chip has broad application prospects in the fields of medical treatment, public health, food safety, etc., especially in the fields of rapid on-site detection, primary medical institutions and areas with limited resources. The chip can provide a convenient and efficient solution for nucleic acid detection. At the same time, with the continuous development and improvement of technology, the chip is expected to be applied in more fields.

[0031] 2. Pre-place the various nucleic acid extraction reagents required in the specific cavity of the lower shell 2 of the reagent kit, and seal them with the sealing rubber 3 to ensure that the reagents will not leak, evaporate or be contaminated by the outside world during storage and transportation, and maintain the stability and effectiveness of the reagents. When performing the test, first move the magnetic rod cover 6 upward so that it does not affect the subsequent operation. Then, by rotating the upper shell 1, the cavity containing the lysate is accurately rotated to the position of the sample injection port 10, which is convenient for operation. Then, add the sample and magnetic beads to the sample injection cavity 11 to prepare for the subsequent nucleic acid lysis reaction. By rotating the upper shell 1, the lysate cavity is in a completely sealed state, which can prevent liquid leakage during the mixing process. Shake the chip by hand , which can make the lysis solution, sample and magnetic beads fully contact, promote the lysis reaction of nucleic acid, release the nucleic acid from the sample and combine with the magnetic beads, rotate the upper shell 1, adjust the sample addition chamber 11 to the position of the magnetic bar 5, insert the magnetic bar 5, let it stand for two minutes, use the magnetism of the magnetic bar cover 6 to adsorb the magnetic beads on the magnetic bar cover 6, so as to achieve the separation of the magnetic beads from other liquid components, move the magnetic bar cover 6 and the magnetic bar 5 upward together to avoid interference with subsequent operations, then rotate the upper shell 1 to align the position of the magnetic bar 5 with the cavity where the washing solution 12 is located, then move the magnetic bar cover 6 downward to make the magnetic beads enter the washing solution 12, and take out the magnetic bar 5. Manual mixing can make the magnetic beads fully clean in the washing solution 12 to remove impurities, and then Move the magnetic rod cover 6 downward, insert the magnetic rod 5, and let it stand for two minutes to allow the magnetic beads to be re-adsorbed on the magnetic rod cover 6 to separate the magnetic beads from the washing solution 12, repeat the above operation in the cavity where the washing solution 2 13 is located, and wash the magnetic beads for the second time to further improve the purity of the nucleic acid, rotate the upper shell 1, align the magnetic rod 5 with the cavity where the eluent 14 is located, repeat the above operation, elute the magnetic beads in the eluent 14, and enter it, finally, move the magnetic rod cover 6 and the magnetic rod 5 upward together, rotate the upper shell 1, and move the cavity containing the eluent 14 to the notch position, so that the eluent 14 can be taken out of the centrifuge tube for use for subsequent nucleic acid detection and analysis. The surface of the lower shell 2 is provided with alignment scale lines 15, which can help The user is helped to accurately rotate the upper shell 1 and the lower shell 2 to specific positions during operation. When the lysate chamber, the elution liquid 14 chamber, the washing liquid 12 and the washing liquid 2 13 chambers are aligned with the positions of the magnetic rod 5, the alignment scale line 15 can provide a clear reference to ensure that each rotation is accurate and in place to avoid operational errors due to inaccurate positions. A rotating sliding button 7 is provided on the surface of the upper shell 1, which makes the action of rotating the upper shell 1 easier and smoother. Compared with directly holding the upper shell 1 by hand for rotation, the button design can better control the force and angle of rotation and improve the accuracy of the operation. The setting of the sample adding chamber 11 provides a specific area for adding samples, making the addition of samples more convenient and centralized.

[0032] 3. The overall multi-step lysis, washing and elution process can effectively extract high-purity nucleic acids. Manual mixing and magnetic bead adsorption separation can ensure the full implementation of each step and improve the extraction efficiency. The reagents are pre-buried in the cavity of the lower shell 2 of the reagent kit and sealed by the sealing rubber 3, which is convenient for use and ensures the stability and safety of the reagents, avoiding waste and pollution of the reagents. The rotary design allows each cavity to accurately adjust its position as needed, which is convenient for operations in different steps. Different reagents and operation sequences can be flexibly selected according to actual conditions to meet different nucleic acid extraction needs. The manual nucleic acid extraction chip does not require expensive instruments and equipment, has low cost, and is suitable for large-scale promotion and application. At the same time, the pre-buried and sealed design of the reagents also reduces the amount of reagents used and reduces costs.

[0033] The examples of the present invention are given for the purpose of illustration and description, and are not intended to be exhaustive or to limit the invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention and to enable those of ordinary skill in the art to understand the present invention and to design various embodiments with various modifications suitable for specific uses.

Claims

1. A rotary manual nucleic acid extraction microfluidic chip, comprising an upper shell (1), a lower shell (2), a sealing rubber (3), a sealing ring (8), a rotary sliding button (7), a magnetic rod sleeve (6) and a magnetic rod (5), characterized in that: The sealing rubber (3) is located on the upper surface of the lower shell (2), the upper shell (1) is located on the upper surface of the sealing rubber (3), the upper surface of the lower shell (2) is provided with a sample loading chamber (11), a washing liquid 1 (12), a washing liquid 2 (13) and an eluent (14), and the annular side surface of the lower shell (2) is provided with a registration scale line (15); The magnetic rod sleeve (6) is moved upward, and then the lysate chamber is rotated to the position of the sample loading port (10), the sample and the magnetic beads are added into the sample loading chamber (11), the upper shell (1) is rotated, and the lysate chamber is rotated to a completely sealed position, the chip is shaken by hand to fully mix the lysate, the sample and the magnetic beads, the upper shell (1) is rotated, and the sample loading chamber (11) is rotated to the position of the magnetic rod (5), and then the magnetic rod (5) is inserted, and it is left to stand for two minutes to allow the magnetic beads to be adsorbed on the magnetic rod sleeve (6).

2. The rotary manual nucleic acid extraction microfluidic chip according to claim 1, characterized in that: The rotating sliding button (7) is arranged on the annular side surface of the upper shell (1).

3. The rotary manual nucleic acid extraction microfluidic chip according to claim 1, characterized in that: Various reagents are pre-buried in the cavity of the reagent box lower shell (2) and sealed by a sealing rubber (3).

4. The rotary manual nucleic acid extraction microfluidic chip according to claim 1, characterized in that: The sealing ring (8) is movably sleeved with the magnetic rod sleeve (6).

5. The rotary manual nucleic acid extraction microfluidic chip as claimed in claim 1, characterized in that: A sample adding notch (9) is provided on the surface of the sealing rubber (3).

6. The rotary manual nucleic acid extraction microfluidic chip according to claim 1, characterized in that: The magnetic bar (5) can be inserted into the magnetic bar sleeve (6) or taken out from the magnetic bar sleeve (6), so that the magnetic bar sleeve (6) can be magnetic or non-magnetic.

7. The rotary manual nucleic acid extraction microfluidic chip according to claim 1, characterized in that: The magnetic rod sleeve (6) is movably sleeved with the lower shell (2).

8. The rotary manual nucleic acid extraction microfluidic chip as claimed in claim 1, characterized in that: The magnetic rod sleeve (6) can be inserted into the hole of the lower shell (2) or taken out from the hole; Wherein, after the magnetic rod sleeve (6) is inserted downward into the cavity, the cavity can be sealed.

9. The rotary manual nucleic acid extraction microfluidic chip as claimed in claim 1, characterized in that: The upper shell (1) and the sealing rubber (3) can rotate relative to each other to achieve the transfer of the magnetic beads.

10. The rotary manual nucleic acid extraction microfluidic chip according to claim 1, characterized in that: Various reagents are pre-buried in the lower shell (2), and the magnetic beads can be mixed and transferred in each hole by rotating the upper shell (1), moving the magnetic rod (5), moving the rotating sliding button (7), and manually mixing.

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