Conjoined extraction tube for virus sampling as well as preparation method and application of conjoined extraction tube

Abstract

The invention provides a conjoined extraction tube for virus sampling as well as a preparation method and application thereof, the conjoined extraction tube for virus sampling comprises a tube body and a water dropper, and the tube body and the water dropper are connected through a lug; the pipe body, the dropper and the lug are made of the same components including LDPE and POE. The tube body, the water dropper and the lug of the extraction tube are mainly composed of LDPE and POE, so that the extraction tube has good toughness, and breakage caused by repeated pressing in the use process of the extraction tube is avoided; in addition, compared with other flexibilizers, the POE not only improves the toughness, but also remarkably improves the liquid barrier property of the extraction tube, reduces the leakage of preservation liquid, and prolongs the shelf life of the extraction tube.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a conjoined extraction tube for virus sampling, a preparation method and application thereof. Background Art

[0002] The invention patent entitled "One-piece extraction tube for virus sampling" with the authorization announcement number CN217733107U and the authorization announcement on November 4, 2022 discloses a one-piece extraction tube for virus sampling, including a tube body and a dripper, wherein the tube body and the dripper are connected by an ear piece, and the dripper is provided with a connecting portion. After the tube body and the dripper are assembled, the connecting portion of the dripper is sealed and connected to the mouth of the tube body, and the outer end surface of the mouth of the tube body is provided with a heat-sealing rib for heat-sealing aluminum foil material. Before the tube body and the dripper are assembled, the mouth of the tube body is heat-sealed with aluminum foil material, and the virus preservation liquid is sealed inside the tube body. There is no need to pour the virus preservation liquid into the tube body at the sampling site, which reduces the operation of the sampling personnel, avoids the problem of spillage of the virus preservation liquid, improves the convenience of the sampling operation, and improves the sampling efficiency. This structure greatly reduces the leakage rate of the aluminum foil seal, improves the problem that the dripper cannot be inserted into the tube body, improves the sealing between the dripper and the tube body, solves the problem of the dripper and the tube body loosening, and effectively improves the yield rate of the product. However, the extraction tube involves the storage of the preservation solution in the tube, and how to further improve the durability and shelf life of the extraction tube is a technical problem that needs to be solved. Summary of the invention

[0003] In view of the problems existing in the background technology, the purpose of the present invention is to provide a one-piece extraction tube for virus sampling, which has good toughness and barrier properties.

[0004] In order to achieve the above-mentioned purpose, the present invention provides a one-piece extraction tube for virus sampling, comprising a tube body and a dripper, wherein the tube body and the dripper are connected by an ear piece; characterized in that the components of the tube body, the dripper and the ear piece are the same, including LDPE and POE.

[0005] Furthermore, the content of the POE is 4-10%; the content of the LDPE is 90-96%.

[0006] Furthermore, the melt index of the LDPE is 60-70.

[0007] Furthermore, the mouth of the tube body is heat-sealed with aluminum foil material.

[0008] The present invention also provides a method for preparing a one-piece extraction tube for virus sampling, comprising the steps of: (1) weighing raw materials containing LDPE and POE according to a weight ratio, homogenizing, and obtaining an injection material; and (2) using an injection machine to inject the injection material into an integral molding to obtain an extraction tube.

[0009] Furthermore, the homogenization includes stirring and mixing or extrusion plasticizing to prepare masterbatch.

[0010] The present invention also provides an application of a conjoined extraction tube for virus sampling, which can be used for the detection of viruses, bacteria or pollutants.

[0011] The beneficial effects of the present invention are as follows:

[0012] 1. The tube body, dripper and ear piece of the extraction tube of the present invention are mainly composed of LDPE and POE, and have good toughness, which can prevent the extraction tube from being broken due to repeated pressing during use; in addition, compared with other toughening agents, POE not only improves the toughness, but also significantly improves the liquid barrier property of the extraction tube, reduces the leakage of the preservation solution, and prolongs the shelf life of the extraction tube.

[0013] 2. POE improves the elasticity of the tube body and the dripper, and can increase the interference fit of the tube body and the dripper to a certain extent. The tube body and the dripper are assembled more tightly, and the friction between the two is higher. The tube body and the dripper are not easy to fall off due to the elasticity of the ear piece, reducing the possibility of leakage at the assembly port.

[0014] 3. Tests have shown that the addition of POE can significantly improve the heat-sealing adhesion between the mouth of the LDPE tube and the aluminum foil material, resulting in better sealing and extended shelf life of the extraction tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the technical solution of the present invention, the drawings required for use in the embodiments are briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0016] Figure 1 Schematic diagram of the three-dimensional structure of an embodiment of a one-piece extraction tube for virus sampling according to the present invention.

[0017] Figure 2 for Figure 1 A schematic cross-sectional diagram of the planar structure of an embodiment of the present invention.

[0018] Figure 3 for Figure 1 A schematic diagram of the three-dimensional structure of the tube body and the dripper after being assembled in an embodiment.

[0019] Figure 4 It is a schematic diagram of the three-dimensional structure of another embodiment of the one-piece extraction tube for virus sampling of the present invention.

[0020] Figure 5 for Figure 4 A schematic diagram of the three-dimensional structure of the tube body and the dripper after being assembled in an embodiment.

[0021] Figure 6 for Figure 1 A schematic cross-sectional view of the planar structure of the tube body and the dripper after assembly of the embodiment.

[0022] Figure 7 for Figure 6 A variation of the embodiment.

[0023] Illustrations: 1-tube body; 11-mouth; 111-heat-sealing rib; 112-chamfer; 12-tube body convex rib; 13-tube body groove; 2-drip head; 21-connecting part; 211-drip head convex rib; 22-top wall; 23-perforation; 24-drip nozzle; 241-top wall; 242-drip hole; 3-ear piece; 31-recess. DETAILED DESCRIPTION

[0024] In order to make the above-mentioned objects, features and advantages of the invention more obvious and easy to understand, the specific embodiments of the invention are described in detail below.

[0025] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0026] Reference Figures 1 to 7 The one-piece extraction tube for virus sampling according to the present invention comprises a tube body 1 and a dripper 2, wherein the dripper 2 is provided with a connecting portion 21. After the tube body 1 and the dripper 2 are assembled, the connecting portion 21 of the dripper 2 is sealed and connected to the mouth 11 of the tube body 1, and a through hole 23 penetrating the top wall 22 is provided on the top wall 22 of the dripper 2, and a drip nozzle 24 connected to the through hole 23 is provided on the top wall 22 of the dripper 2, and a drip hole 242 is provided on the top wall 241 of the drip nozzle 24, and the tube body 1 and the dripper 2 are connected by an ear piece 3 and are integrally injection molded.

[0027] The outer end surface of the mouth 11 of the tube body 1 is provided with a heat-sealing rib 111 for heat-sealing aluminum foil material. Before the tube body 1 and the dripper 2 are assembled, the mouth 11 of the tube body 1 is heat-sealed with aluminum foil material, and the virus preservation liquid is sealed inside the tube body 1. In actual production, in order to improve the heat-sealing efficiency, multiple extraction tubes are heat-sealed simultaneously during heat sealing, which places high requirements on the height dimension of the heat-sealing surface of the extraction tube. Assuming that the heat-sealing surface heights of multiple extraction tubes are different, the extraction tube with a high height will be heat-sealed first during heat sealing, which will cause the heat sealing of the extraction tube with a low height to be insufficient, and even cause the extraction tube with a low height to leak. The present invention provides a circle of thinner heat-sealing ribs on the heat-sealing surface. When the heat-sealing surface heights of the extraction tubes are different, the heat-sealing head first contacts the heat-sealing ribs of the extraction tube with a high height. Since the ribs are thinner, the ribs can be heat-melted in a very short time, and then the extraction tube with a low height is heat-melted, so the leakage defect rate of the heat-sealing of the tube body mouth can be effectively reduced.

[0028] The present invention heat-seals the aluminum foil material at the mouth 11 of the tube body 1 to seal the virus preservation solution inside the tube body 1. It is not necessary to pour the virus preservation solution into the tube body at the sampling site, which reduces the operation of the sampling personnel, avoids the problem of virus preservation solution spilling, improves the convenience of sampling operation, and improves sampling efficiency.

[0029] Reference Figure 1 , Figure 2 and Figure 4 , the upper end surface of the ear piece 3 is provided with a recess 31. When the extraction tube is in use, the dripper 2 needs to be inserted into the tube body 1 for use. When the dripper 2 is inserted into the tube body 1, the ear piece 3 is in a twisted state and has a rebound force. The dripper 2 is easily bounced out of the tube body 1, affecting the use. The recess 31 is provided on the ear piece 3 of the present invention, which will greatly reduce the rebound force of this part. There is no limitation on the shape and position of the recess 31 of the ear piece 3 of the present invention, as long as the effect of reducing the rebound force of this part can be achieved, it conforms to the concept of the present invention.

[0030] Reference Figure 1 , Figure 2 and Figure 4 , the mouth 11 of the tube body 1 is provided with a chamfer 112. During heat sealing, the mouth 11 of the tube body 1 will be melted, and under the action of pressure, the hot melt plastic will be pushed into the mouth 11, which will cause the inner diameter of the mouth 11 to become smaller, which may easily cause the dripper 2 to fail to be inserted into the tube body 1, resulting in defects. By providing a chamfer, when the hot melt plastic is pushed into the mouth 11, the inner diameter of the actual matching mouth 11 will not be reduced, which can effectively reduce the defect of the dripper 2 failing to be inserted into the tube body 1.

[0031] In the one-piece extraction tube for virus sampling according to the present invention, the connecting portion 21 of the dripper 2 is connected with the mouth 11 of the tube body 1 by interference fit, so that the tube body 1 and the dripper 2 can be matched, so that the tube body and the dripper can be assembled together when in use, and when inverted, the assembly mouth does not leak liquid.

[0032] Reference Figure 1 , Figure 2 , Figure 4 , Figure 6 and Figure 7 The outer side of the connection part 21 of the dripper 2 and the inner side of the mouth 11 of the tube body 1 are provided with mutually matching anti-drop structures. The dripper 2 and the tube body 1 are provided with anti-drop structures, and the anti-drop structures can be convex ribs clamped in convex ribs, or convex ribs clamped in grooves. In one embodiment, refer to Figure 1 , Figure 2 , Figure 4 , Figure 6 The outer side of the connection portion 21 of the dripper 2 is provided with a dripper convex rib 211, and the inner side of the mouth portion 11 of the tube body 1 is provided with a tube body convex rib 12 that matches the dripper convex rib 211. In another embodiment, referring to Figure 7 , a dripper convex rib 211 is provided on the outside of the connection portion 21 of the dripper 2, and a tube body groove 12 cooperating with the dripper convex rib 211 is provided on the inside of the mouth 11 of the tube body 1. In another embodiment, a dripper groove (not shown) is provided on the outside of the connection portion 21 of the dripper 2, and a tube body convex rib cooperating with the dripper convex rib 211 is provided on the inside of the mouth 11 of the tube body 1. In the "One-piece extraction tube for virus sampling and its removal fixture" disclosed in the patent with authorization announcement number CN214830328U, the cooperation between the dripper and the tube body of the extraction tube adopts a full-circle interference fit to achieve the requirement that the dripper does not fall out of the tube body, which increases the difficulty of mold development and injection molding process control. The present invention adopts an anti-slip structure, which can prevent product defects and reduce the difficulty of mold development and injection molding process control.

[0033] Reference Figure 2 There is a height difference of 0.3-0.5 cm between the ear piece 3 and the outer end surface of the mouth 11 of the tube body 1. When the outer end surface of the mouth 11 of the tube body 1 is heat-sealed, due to the position of the extraction tube, the accuracy of the equipment, etc., the coverage area of ​​the heat-sealing head may reach the ear piece 3, and the ear piece 3 is easily melted by heat at this time, causing defects. If a height difference of 0.3-0.5 cm is set, the heat-sealing head can effectively avoid the ear piece 3 from being melted by heat.

[0034] The dripping amount of the dripping hole of the drip nozzle 24 of the present invention is 30±5uL / drop.

[0035] The invention facilitates the detection personnel to squeeze the virus sampling liquid in the tube body from the drip hole through the cooperation of the drip nozzle and the tube body, thereby reducing the spillage of the virus sampling liquid, thereby improving the safety of the detection personnel during operation and sampling, and improving the detection accuracy. Moreover, when the sampling personnel squeezes the tube body, it is convenient for the sampling personnel to take an appropriate amount, thereby improving the reliability of sampling.

[0036] In one embodiment of the one-piece extraction tube for virus sampling according to the present invention, Figures 1 to 3 , the conjoined extraction tube is a single-ear conjoined extraction tube, that is, the number of the ear pieces is 1. In another embodiment, referring to Figures 4 to 5 The one-piece extraction tube is a two-ear one-piece extraction tube, that is, the number of ear pieces is 2.

[0037] In one embodiment of the one-piece extraction tube for virus sampling according to the present invention, the tube body, the dripper and the ear piece can be made of a transparent flexible material. The flexible material is convenient for the sampler to squeeze the tube body, and the extraction tube can be repeatedly squeezed to quickly rebound and return to the status quo. The transparent flexible material is a transparent plastic, which is convenient for the sampler to observe the swab rod immersed in the swab virus preservation liquid. It is also convenient for the tester to squeeze the bottle body and observe the amount of swab virus sampling liquid in the tube body squeezed out from the drip hole.

[0038] In one embodiment, the transparent flexible material may be transparent plastic.

[0039] In one embodiment, the transparent plastic is preferably a combination of low-density polyethylene (LDPE) and polyolefin elastomer (POE); preferably, the content of the POE is 4-10%, and the content of the LDPE is 90-96%.

[0040] In one embodiment, the melt index of the LDPE is 60-70.

[0041] In one embodiment of the present invention, the present invention also provides a method for preparing a one-piece extraction tube for virus sampling, which is characterized in that it comprises the following steps: (1) weighing raw materials containing LDPE and POE according to a weight ratio, and homogenizing them to obtain an injection material; and (2) injecting the injection material into an integral molding using an injection machine to obtain an extraction tube.

[0042] In one embodiment, the homogenization includes stirring and mixing or extrusion plasticizing to prepare a masterbatch.

[0043] The one-piece extraction tube for virus sampling provided by the present invention is disposable.

[0044] The one-piece extraction tube for virus sampling provided by the invention can be used for the detection of viruses, bacteria or pollutants.

[0045] The present invention is further described in detail by examples below, but these examples should not be considered as limiting the present invention. Main raw materials and equipment used: Unless otherwise specified, the raw materials and equipment of each embodiment and comparative example are the same; materials without specifying specific models or types are purchased from the market or commonly available common models, and are not specifically limited.

[0046] Example 1

[0047] (1) Weigh 90 parts of LDPE and 10 parts of POE by weight, stir in a mixer at a speed of 500-1000 rpm for 10-20 minutes to obtain an injection material;

[0048] (2) Adding the injection material into the barrel of the injection machine, setting the barrel temperature of the injection machine: zone 1 165° C., zone 2 175° C., zone 3 180° C., nozzle 185° C., mold temperature 45° C., injection pressure 1000 bar, holding pressure 750 bar, and performing one-piece injection molding to obtain an extraction tube;

[0049] (3) Add preservation solution and heat-seal the aluminum foil material at the mouth of the extraction tube to obtain the sample.

[0050] Example 2

[0051] Except for 96 parts of LDPE and 4 parts of POE, the rest is the same as Example 1.

[0052] Example 3

[0053] Except for 93 parts of LDPE and 7 parts of POE, the rest is the same as Example 1.

[0054] Comparative Example 1

[0055] Except for 100 parts of LDPE and no POE, the other parts are the same as Example 1.

[0056] Performance Testing

[0057] 1. XLW-H intelligent electronic tensile testing machine, the vacuum generator fixes the tube body, the opening of the aluminum foil material is fixed on the fixture, and the peeling strength between the aluminum foil material and the tube mouth is tested. The tensile speed is 3m / min. The maximum force value is recorded as the opening force, and the average value during the peeling process is the sealing opening force;

[0058] 2. Low temperature fatigue test of the extraction tube: keep the sample temperature at -25°C, and repeatedly press the extraction tube prepared in step (2) until the tube is flat, 30 times, and the frequency is 1 time / second;

[0059] 3. Weigh the sample mass M0 of step (3), place the sample in a vacuum dryer, vacuum degree -0.1Mpa, temperature 75°C, time 48h, take out and cool to room temperature, weigh the sample mass M1, calculate the mass loss rate γ = (M 0- M1) / M0×100%.

[0060] The above performance test results are shown in Table 1.

[0061] Table 1 Performance test results

[0062] Test items Opening force, N Seal opening force, N Appearance after fatigue test Mass loss rate γ Example 1 21 9 Transparent, no white marks 12％ Example 2 19 8.3 Transparent, no white marks 13％ Example 3 22 9.5 Transparent, no white marks 9.5％ Comparative Example 1 15 6.2 Cracks at the crease 20％

[0063] As can be seen from Table 1, the tube body of the present invention is composed of LDPE and POE in a specific ratio. Compared with the LDPE in the prior art, the peel strength between the tube body and the aluminum foil material can be significantly improved and is within a suitable range. Under the condition of maintaining good sealing, it does not hinder opening and use; the toughness and fatigue resistance at low temperatures are good, meeting the use requirements of low temperature environment areas; the barrier properties are good, and the loss rate of the preservation liquid is small, indicating that POE can significantly improve the liquid barrier properties of LDPE.

[0064] Although some specific embodiments of the present invention have been described in detail by way of example, it will be appreciated by those skilled in the art that the above examples are for illustration only and are not intended to limit the scope of the present invention. It will be appreciated by those skilled in the art that the above embodiments may be modified without departing from the scope and spirit of the present invention. The scope of the present invention is defined by the appended claims.

Claims

1. A one-piece extraction tube for virus sampling, comprising a tube body and a dripper, wherein the tube body and the dripper are connected by an ear piece; characterized in that: The components of the tube body, the dripper and the ear piece are the same, including LDPE and POE.

2. The one-piece extraction tube for virus sampling according to claim 1, characterized in that: The content of the POE is 4-10%; the content of the LDPE is 90-96%.

3. The one-piece extraction tube for virus sampling according to claim 1, characterized in that: The melt index of the LDPE is 60-70 g / min (ASTM D1238).

4. The one-piece extraction tube for virus sampling according to claim 1, characterized in that: The mouth of the tube body is heat-sealed with aluminum foil material.

5. The method for preparing a one-piece extraction tube for virus sampling according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: (1) weighing raw materials containing LDPE and POE according to a weight ratio, homogenizing, and obtaining an injection material; and (2) using an injection machine to perform one-piece injection molding on the injection material to obtain an extraction tube.

6. The method for preparing the one-piece extraction tube for virus sampling according to claim 4, characterized in that: The homogenization includes stirring and mixing or extrusion plasticization to prepare masterbatch.

7. Use of the one-piece extraction tube for virus sampling obtained by the preparation method of claims 1-4 or claims 5-6, characterized in that: Can be used for the detection of viruses, bacteria or pollutants.

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