Magnetically-driven cell culture stirrer

Abstract

The invention discloses a magnetically-driven cell culture stirrer, and relates to the technical field of cell culture stirring, the magnetically-driven cell culture stirrer comprises a shell, a support column, a locking seat, a transverse plate, a thermometer and a fixing bolt, the top of the shell is provided with a circular groove, the interior of the circular groove is fixedly connected with a placing table, the interior of the shell is provided with a rotating ring plate, the rotating ring plate is located below the placing table, and the rotating ring plate is located below the placing table. And a first magnetic block is arranged at the top of the rotating ring plate. According to the invention, the stirring area of the rotor on a culture solution and cells in the culture bottle in the horizontal direction and the vertical direction is effectively enlarged, so that nutrient substances can be more quickly and uniformly diffused in the culture bottle, and sufficient nutrient supply around the cells is ensured all the time; meanwhile, through stirring of the rotor, the situation that cell growth and functions are affected due to uneven supply of nutrient substances and oxygen among cells possibly caused by cell aggregation in the culture bottle can be effectively avoided.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture stirring, and in particular to a magnetically driven cell culture stirrer. Background Art

[0002] Cell culture refers to a technology that simulates the in vivo environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to allow cells to survive, grow, reproduce and maintain their main structures and functions.

[0003] In the process of cell culture, in order to promote the exchange of nutrients between cells, maintain gas balance, prevent cell aggregation, and ensure uniform temperature, it is generally necessary to stir the culture medium. During the cell culture process, traditional stirring devices are prone to mechanical contamination. At the same time, the stirring intensity is too high and has a high shear force, which can easily damage the cells. Therefore, magnetic stirrers are often used to stir the cells during the cell culture process.

[0004] After searching, the Chinese invention patent with announcement number CN115532134A discloses a heating magnetic stirrer, a stirring drive device, a temperature measuring device and a temperature control device. The stirring drive device is used to mix and stir the solution in the beaker so that the temperature distribution of the solution in the beaker is more uniform; the temperature measuring device includes a solution temperature measuring device and a heating table temperature measuring device. The solution temperature measuring device is used to measure the temperature of the solution in the beaker and use it as the feedback of the main loop; the heating table temperature measuring device is used to measure the temperature of the heating table and use it as the feedback of the secondary loop; the temperature control device adopts a fuzzy-cascade PID control method to accurately control the temperature of the solution in the beaker.

[0005] Based on the prior art, the above patent still has the following shortcomings: since the magnet spacing in the magnetic stirrer is the same and constant, the stirring area and area of ​​the magnetic stirring assembly are constant when stirring the beaker, resulting in poor mixing effect of the entire magnetic stirrer, which cannot meet people's demand for uniform stirring. Therefore, a magnetically driven cell culture stirrer is urgently needed to solve the above problems. Summary of the invention

[0006] The purpose of the present invention is to solve the shortcomings of the prior art and propose a magnetically driven cell culture agitator. Its advantages are: effectively expanding the stirring area of ​​the rotor for the culture fluid and cells in the culture bottle in the horizontal and vertical directions, so that nutrients can be diffused more quickly and evenly in the culture bottle, ensuring that there is always sufficient nutrient supply around the cells. At the same time, the stirring of the rotor can effectively avoid the aggregation of cells in the culture bottle, which may cause uneven supply of nutrients and oxygen between cells and affect cell growth and function.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions: A magnetically driven cell culture stirrer comprises a shell, a support column, a locking seat, a horizontal plate, a thermometer and fixing bolts, a circular groove is provided on the top of the shell, a placing table is fixedly connected inside the circular groove, a rotating ring plate is provided inside the shell, the rotating ring plate is located below the placing table, a first magnetic block is provided on the top of the rotating ring plate, the number of the first magnetic blocks is two groups, and the two groups of first magnetic blocks are symmetrically distributed; A rotating assembly for driving the two groups of first magnetic blocks to rotate is arranged inside the housing; A guide assembly is provided below the rotating ring plate to enable the first magnetic block to move horizontally when rotating; A clamping assembly for fixing the culture bottle is arranged above the shell; A rotor is placed at the bottom of the culture bottle, and the rotor and the first magnetic block attract each other.

[0008] Through the above technical solution: the stirring area of ​​the rotor on the culture medium and cells in the culture bottle in the horizontal and vertical directions is effectively expanded, so that the nutrients can be diffused more quickly and evenly in the culture bottle, ensuring that there is always sufficient nutrient supply around the cells. At the same time, the stirring of the rotor can effectively avoid the aggregation of cells in the culture bottle, which may cause uneven supply of nutrients and oxygen between cells and affect cell growth and function.

[0009] The present invention is further configured such that the rotating assembly includes a reinforcement frame fixedly connected to the inner wall of one side of the shell, the reinforcement frame is fixedly connected to a motor, the output end of the motor is fixedly connected to a driving gear, the circumferential outer wall of the driving gear is meshed with a driven gear, the circumferential inner wall of the driven gear is fixedly connected to a first rotating column, and the rotating ring plate is fixedly connected to the circumferential outer wall of the first rotating column.

[0010] Through the above technical solution: through the attraction between the first magnetic block and the rotor, the rotor can make a circular motion in the culture bottle, so as to stir the culture solution and cells in the culture bottle, so that the nutrients can be diffused more quickly and evenly in the culture bottle.

[0011] The present invention is further configured as follows: the guide assembly includes a fixed plate fixedly connected to the inner walls on both sides of the shell, one end of the fixed plate is fixedly connected to a circular ring plate, the top outer wall of the circular ring plate is fixedly connected to a connecting column, the top of the connecting column is fixedly connected to a guide rail, one side of the guide rail is fixedly and rollingly connected to a second roller, both sides of the second roller are rotatably connected to a limiting frame, a notch is provided at the bottom of the limiting frame, one end of the limiting frame is fixedly connected to a connecting plate, the top outer wall of the connecting plate is rotatably connected to a rotating shaft, one end of the rotating shaft away from the connecting plate is rotatably connected to the bottom outer wall of the first magnetic block, the circumferential inner wall of the rotating ring plate is fixedly connected to a cross bar, the top outer wall of the cross bar is provided with a limiting groove, and one end of the rotating shaft passes through the inside of the limiting groove.

[0012] Through the above technical solution: the rotation path of the rotor in the culture bottle is not constant, but reciprocating inward and outward, thereby effectively expanding the rotation radius of the rotor in the culture bottle, and further stirring the culture solution and cells in the culture bottle more evenly.

[0013] The present invention is further configured such that the guide rail includes a first arc portion, a second arc portion, and a third arc portion, and the number of the first arc portion, the second arc portion, and the third arc portion are all four groups, and the four groups of the first arc portion, the second arc portion, and the third arc portion are connected end to end to form a closed track.

[0014] Through the above technical solution, the first magnetic block can achieve four diameter changes during one circular motion.

[0015] The present invention is further configured such that a first bevel gear is fixedly connected to a circumferential outer wall of the first rotating column, and a second bevel gear is meshed with a circumferential outer wall of the first bevel gear.

[0016] Through the above technical solution: through the mutual meshing between the first bevel gear and the second bevel gear, when the first bevel gear rotates, it can drive the second bevel gear to rotate stably.

[0017] The present invention is further configured such that the clamping assembly includes a second rotating column fixedly connected to the circumferential inner wall of the second bevel gear, one end of the second rotating column is fixedly connected to a threaded rod, the circumferential outer wall of the threaded rod is threadedly connected to a threaded sleeve, the circumferential outer wall of the threaded sleeve is fixedly connected to a vertical rod, the vertical rod extends to the outer walls on both sides of the shell and is fixedly connected to an arc rod, the arc rod is fixedly connected to a clamping frame, two groups of clamping frames clamp and fix the culture bottle, the bottom inner wall of the shell is fixedly connected to a fixed frame, and the second rotating column is rotatably connected to the fixed frame.

[0018] Through the above technical scheme: it is ensured that the culture bottle can always remain stable during the stirring operation, avoiding the situation where the culture bottle is tipped over or tilted due to excessive stirring amplitude. At the same time, the center of the culture bottle is always the same as the center of the rotating ring plate, ensuring the stirring effect of the rotor on the culture solution and cells in the culture bottle.

[0019] The present invention is further configured such that a rectangular groove is formed on the top outer wall of the shell, and one end of the vertical rod passes through the inside of the rectangular groove.

[0020] Through the above technical solution: the rectangular groove can play a good limiting and guiding role in the horizontal movement of the vertical rod.

[0021] The present invention is further configured such that a vertical plate is fixedly connected to the bottom inner wall of the shell, one end of the second rotating column passes through one side of the vertical plate, a second spring is fixedly connected to an outer wall of one side of the vertical plate, one end of the threaded sleeve is fixedly connected to a circular plate, and an end of the threaded sleeve away from the circular plate is located in the same vertical plane as one end of the threaded rod.

[0022] Through the above technical solution: the second spring can always give the threaded sleeve a lateral thrust, and when the threaded rod rotates in the opposite direction, the threaded sleeve can quickly form a threaded connection with it, thereby driving the clamping frame to move away from the culture bottle, releasing the fixing effect on the culture bottle.

[0023] The present invention is further configured as follows: the circumferential outer wall of the second rotating column is fixedly connected with a cam, the circumferential inner wall of the placing table is fixedly connected with a guide cylinder, a push rod is inserted into the inside of the guide cylinder, the bottom outer wall of the guide cylinder is fixedly connected with a first spring, the bottom end of the push rod is fixedly connected with a rolling seat, the inner walls on both sides of the rolling seat are rotatably connected with the first rollers, the first rollers are in contact with the circumferential outer wall of the cam, the end of the first spring away from the guide cylinder is fixedly connected to the top outer wall of the rolling seat, the circumferential inner wall of the clamping frame is fixedly connected with the guide column, the circumferential outer wall of the guide column is sleeved with a sliding sleeve, one side of the sliding sleeve is fixedly connected with a second magnetic block, the bottom of the second magnetic block is fixedly connected with a docking seat, the top of the push rod cooperates with the docking seat, and the bottom of the clamping frame and the top of the placing table are both provided with through grooves.

[0024] Through the above technical solution, the rotor is not limited to stirring the culture solution and cells at the bottom of the culture bottle, and the vertical stirring area of ​​the rotor for the culture solution and cells in the culture bottle is expanded.

[0025] The present invention is further configured such that the rotor comprises a spherical portion and a supporting portion, the spherical portion is in two groups, and the two groups of spherical portions are respectively arranged on both sides of the supporting portion.

[0026] Through the above technical solution: the spherical part not only reduces the shear force on the cells during stirring, avoiding damage to the cells during stirring, but also the spherical part can expand the area of ​​attraction between the second magnetic block, so that the rotor can stably follow the second magnetic block to rise or fall.

[0027] The beneficial effects of the present invention are: 1. The present invention can make the rotor perform circular motion in the culture bottle through the attraction between the first magnetic block and the rotor, thereby achieving stirring of the culture solution and cells in the culture bottle, so that nutrients can be diffused more quickly and evenly in the culture bottle, ensuring that there is always sufficient nutrient supply around the cells. At the same time, the stirring of the rotor can effectively avoid the aggregation of cells in the culture bottle, which may cause uneven supply of nutrients and oxygen between cells and affect cell growth and function.

[0028] 2. The present invention effectively expands the rotation radius of the rotor in the culture bottle, thereby stirring the culture fluid and cells in the culture bottle more evenly. Specifically, a guide component is provided. In the process that the two groups of first magnetic blocks follow the rotating ring plate to make a circular motion together, the roller will roll along the outer wall of the guide rail. In the process that the roller rolls, it can drive the limit frame, the connecting plate and the first magnetic block to move together. However, a limit groove is provided on the top of the cross bar. In the process that the first magnetic block follows the roller to move, the rotating shaft will be limited by the limit groove, thereby driving the first magnetic block to reciprocate along the direction of the limit groove. When the two groups of magnetic blocks move toward each other, the rotation radius of the rotor in the culture bottle will also be reduced accordingly. When the two groups of magnetic blocks move away from each other, the rotation radius of the rotor in the culture bottle will also be increased accordingly, so that the rotation path of the rotor in the culture bottle is not constant, but reciprocates inward and outward, thereby expanding the stirring area of ​​the rotor on the culture fluid and cells in the culture bottle in the horizontal direction.

[0029] 3. The present invention can clamp and fix the culture bottle quickly when the stirrer is started through the clamping assembly, thereby ensuring that the culture bottle can always remain stable during the stirring operation, avoiding the situation that the culture bottle is tipped over or tilted due to excessive stirring amplitude, and at the same time making the center of the culture bottle always the same as the center of the rotating ring plate, thereby ensuring the stirring effect of the rotor on the culture solution and cells in the culture bottle. After the clamping frame clamps and fixes the culture bottle, as the first rotating column continues to rotate, the second rotating column will also continue to make a circular motion. When the second rotating column drives the cam to make a circular motion, the cam will gradually lift the first roller and the push rod upward. As the push rod is lifted upward, the second magnetic block located in the clamping frame can be driven to rise together. At the same time, an attraction force will be generated between the second magnetic block and the rotor. When the rotor rotates to the vicinity of the second magnetic block, it will be lifted by the attraction force of the second magnetic block, thereby not being limited to the stirring of the culture solution and cells at the bottom of the culture bottle, and expanding the stirring area of ​​the rotor on the culture solution and cells in the culture bottle in the vertical direction. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a schematic diagram of the overall front structure of a magnetically driven cell culture agitator proposed by the present invention; Figure 2 This is a schematic diagram of the overall back structure of a magnetically driven cell culture agitator proposed by the present invention; Figure 3 This is a schematic diagram of the disassembled structure of the clamping assembly of a magnetically driven cell culture agitator proposed by the present invention; Figure 4 A schematic diagram of the bottom structure of a placement table for a magnetically driven cell culture agitator proposed by the present invention; Figure 5 A magnetically driven cell culture agitator proposed by the present invention Figure 4 A schematic diagram of the enlarged structure at A in the middle; Figure 6 A magnetically driven cell culture agitator proposed by the present invention Figure 4 A schematic diagram of the enlarged structure at B in the middle; Figure 7 This is a schematic diagram of the disassembled structure of the clamping assembly of a magnetically driven cell culture agitator proposed by the present invention; Figure 8 A magnetically driven cell culture agitator proposed by the present invention Figure 7 Schematic diagram of the enlarged structure at C in the middle; Fig. 9 This is a schematic diagram of the structure of a rotating ring plate of a magnetically driven cell culture agitator proposed by the present invention; Fig.10 A magnetically driven cell culture agitator proposed by the present invention Fig. 9 Schematic diagram of the enlarged structure at D in the middle; Fig.11 This is a schematic diagram of the enlarged structure of the guide rail of a magnetically driven cell culture agitator proposed by the present invention; Fig.12 This is a schematic diagram of the overall half-section structure of a magnetically driven cell culture agitator proposed by the present invention; Fig.13 A magnetically driven cell culture agitator proposed by the present invention Fig.12 Schematic diagram of the enlarged structure at E in the middle.

[0031] In the figure: 1, shell; 2, culture bottle; 3, support column; 4, clamping frame; 5, arc rod; 6, rectangular groove; 7, vertical rod; 8, horizontal plate; 9, thermometer; 10, locking seat; 11, fixing bolt; 12, placing table; 13, circular groove; 14, fixing plate; 15, reinforcing frame; 16, vertical plate; 17, circular ring plate; 18, rotating ring plate; 19, driving gear; 20, motor; 21, driven gear; 22, first rotating column; 23, fixing frame; 24, first spring; 25, rolling seat; 26, first roller; 27, circular plate; 28. Cam; 29. ​​Second spring; 30. Threaded sleeve; 31. First bevel gear; 32. Second bevel gear; 33. Threaded rod; 34. Second rotating column; 35. First magnetic block; 36. Cross bar; 37. Push rod; 38. Second magnetic block; 39. Guide column; 40. Through slot; 41. Limiting slot; 42. Guide rail; 4201. First arc-shaped portion; 4202. Second arc-shaped portion; 4203. Third arc-shaped portion; 43. Connecting plate; 44. Limiting frame; 45. Second roller; 46. Connecting column; 47. Guide cylinder; 48. Rotor. DETAILED DESCRIPTION

[0032] The technical solution of this patent is further described in detail below in conjunction with specific implementation methods.

[0033] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0034] In the description of this patent, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing this patent and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this patent.

[0035] In the description of this patent, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", and "set" should be understood in a broad sense, for example, it can be fixedly connected or set, or it can be detachably connected or set, or connected or set in one piece. For ordinary technicians in this field, the specific meanings of the above terms in this patent can be understood according to specific circumstances.

[0036] Reference Figure 1-Figure 13A magnetically driven cell culture stirrer comprises a housing 1, a support column 3, a locking seat 10, a horizontal plate 8, a thermometer 9 and a fixing bolt 11. A circular groove 13 is provided on the top of the housing 1. A placing table 12 is fixedly connected inside the circular groove 13. A rotating ring plate 18 is provided inside the housing 1. The rotating ring plate 18 is located below the placing table 12. A first magnetic block 35 is provided on the top of the rotating ring plate 18. The number of the first magnetic blocks 35 is two groups, and the two groups of first magnetic blocks 35 are symmetrically distributed. The housing 1 is provided with a rotating assembly for driving the two groups of first magnetic blocks 35 to rotate; A guide assembly is provided below the rotating ring plate 18 to enable the first magnetic block 35 to move horizontally when rotating; A clamping assembly for fixing the culture bottle 2 is provided above the housing 1; A rotor 48 is placed at the bottom of the culture bottle 2. The rotor 48 and the first magnetic block 35 attract each other, effectively expanding the stirring area of ​​the rotor 48 on the culture solution and cells in the culture bottle 2 in the horizontal and vertical directions, so that nutrients can be diffused more quickly and evenly in the culture bottle 2, ensuring that there is always sufficient nutrition supply around the cells. At the same time, the stirring of the rotor 48 can also effectively avoid the aggregation of cells in the culture bottle 2, which may cause uneven supply of nutrients and oxygen between cells and affect cell growth and function.

[0037] In order to ensure that the rotor 48 can stably rotate in the culture bottle 2, refer to Figure 7 The rotating assembly includes a reinforcing frame 15 fixedly connected to the inner wall of one side of the shell 1, the reinforcing frame 15 is fixedly connected to the motor 20, the output end of the motor 20 is fixedly connected to a driving gear 19, the circumferential outer wall of the driving gear 19 is meshed with a driven gear 21, the circumferential inner wall of the driven gear 21 is fixedly connected to a first rotating column 22, and the rotating ring plate 18 is fixedly connected to the circumferential outer wall of the first rotating column 22. The motor 20 drives the driving gear 19 to rotate, and the driving gear 19 will mesh with the driven gear 21 during the rotation process, thereby driving the first rotating column 22 to rotate. During the rotation of the first rotating column 22, the rotating ring plate 18 and the two groups of first magnetic blocks 35 can be driven to rotate together. Through the attraction between the first magnetic block 35 and the rotor 48, the rotor 48 can make a circular motion in the culture bottle 2, thereby achieving the stirring of the culture solution and cells in the culture bottle 2, so that the nutrients can be diffused more quickly and evenly in the culture bottle 2.

[0038] In order to expand the stirring area of ​​the rotor 48 in the culture bottle 2 in the horizontal direction, refer to Figure 7-Figure 11The guide assembly includes a fixed plate 14 fixedly connected to the inner walls of both sides of the housing 1, one end of the fixed plate 14 is fixedly connected to a circular plate 17, the top outer wall of the circular plate 17 is fixedly connected to a connecting column 46, the top of the connecting column 46 is fixedly connected to a guide rail 42, one side of the guide rail 42 is fixedly and rollingly connected to a second roller 45, both sides of the second roller 45 are rotatably connected to a limit frame 44, the bottom of the limit frame 44 is provided with a notch, one end of the limit frame 44 is fixedly connected to a connecting plate 43, the top outer wall of the connecting plate 43 is rotatably connected to A rotating shaft is connected, and one end of the rotating shaft away from the connecting plate 43 is rotatably connected to the bottom outer wall of the first magnetic block 35. The circumferential inner wall of the rotating ring plate 18 is fixedly connected to the cross bar 36. The top outer wall of the cross bar 36 is provided with a limiting groove 41. One end of the rotating shaft passes through the inside of the limiting groove 41. The guide rail 42 includes a first arc portion 4201, a second arc portion 4202, and a third arc portion 4203. The number of the first arc portion 4201, the second arc portion 4202 and the third arc portion 4203 is four groups. The four groups of the first arc portion 4201, the second arc portion 4202 and the third arc portion 4203 are connected end to end to form a closed track. When the two groups of first magnetic blocks 35 follow the rotating ring plate 18 to make a circular motion together, the roller will roll along the outer wall of the guide rail 42. During the rolling of the roller, the limit frame 44, the connecting plate 43 and the first magnetic block 35 can be driven to move together. However, a limit slot 41 is provided on the top of the cross bar 36. When the first magnetic block 35 follows the roller to move, the rotating shaft will be restricted by the limit slot 41, thereby driving the first The magnetic block 35 reciprocates along the direction of the limiting groove 41. When the two groups of magnetic blocks move toward each other, the rotation radius of the rotor 48 located in the culture bottle 2 will also decrease accordingly. When the two groups of magnetic blocks move away from each other, the rotation radius of the rotor 48 located in the culture bottle 2 will also increase accordingly, so that the rotation path of the rotor 48 in the culture bottle 2 is not constant, but reciprocates inward and outward, thereby effectively expanding the rotation radius of the rotor 48 in the culture bottle 2, and then more evenly stirring the culture fluid and cells in the culture bottle 2.

[0039] In order to ensure the stable rotation of the second bevel gear 32, refer to Figure 5 The circumferential outer wall of the first rotating column 22 is fixedly connected with the first bevel gear 31, and the circumferential outer wall of the first bevel gear 31 is meshed with the second bevel gear 32. Through the mutual meshing between the first bevel gear 31 and the second bevel gear 32, when the first bevel gear 31 rotates, it can drive the second bevel gear 32 to rotate stably.

[0040] To ensure that the culture bottle 2 remains stable during stirring, refer to Figure 3-Figure 7The clamping assembly includes a second rotating column 34 fixedly connected to the circumferential inner wall of the second bevel gear 32, one end of the second rotating column 34 is fixedly connected to a threaded rod 33, the circumferential outer wall of the threaded rod 33 is threadedly connected to a threaded sleeve 30, the circumferential outer wall of the threaded sleeve 30 is fixedly connected to a vertical rod 7, the vertical rod 7 extends to the outer walls of both sides of the shell 1 and is fixedly connected to an arc rod 5, the arc rod 5 is fixedly connected to a clamping frame 4, the two groups of clamping frames 4 clamp the culture bottle 2, the bottom inner wall of the shell 1 is fixedly connected to a fixed frame 23, the second rotating column 34 is rotatably connected to the fixed frame 23, when the first rotating column 22 rotates, it will drive the first bevel gear 31 to rotate, through the first The mutual engagement between the first bevel gear 31 and the second bevel gear 32 can drive the second rotating column 34 and the threaded rod 33 to rotate together. During the rotation of the threaded screw, the vertical rod 7 and the clamping frame 4 of the threaded sleeve 30 threadedly connected to its outer wall can move together, so that the culture bottle 2 can be quickly clamped and fixed when the agitator is started, ensuring that the culture bottle 2 can always remain stable during the stirring operation, avoiding the situation where the culture bottle 2 is tipped over or tilted due to excessive stirring amplitude, and at the same time, the center of the culture bottle 2 is always the same as the center of the rotating ring plate 18, ensuring the stirring effect of the rotor 48 on the culture solution and cells in the culture bottle 2.

[0041] In order to make the vertical rod 7 to be able to do horizontal movement stably, refer to Figure 3-Figure 7 A rectangular groove 6 is provided on the top outer wall of the shell 1 , and one end of the vertical rod 7 passes through the inside of the rectangular groove 6 . The rectangular groove 6 can play a good limiting and guiding role for the horizontal movement of the vertical rod 7 .

[0042] In order to ensure that the threaded sleeve 30 can be stably reset when the agitator is stopped, refer to Figure 4-Figure 8 The bottom inner wall of the shell 1 is fixedly connected with a vertical plate 16, one end of the second rotating column 34 passes through one side of the vertical plate 16, and the outer wall of one side of the vertical plate 16 is fixedly connected with a second spring 29. One end of the threaded sleeve 30 is fixedly connected with the circular plate 27. The second spring 29 can always give a lateral thrust to the threaded sleeve 30. When the threaded rod 33 rotates in the opposite direction, the threaded sleeve 30 can quickly form a threaded connection with it, thereby driving the clamping frame 4 to move away from the culture bottle 2, releasing the fixing effect on the culture bottle 2, and the end of the threaded sleeve 30 away from the circular plate 27 is located in the same vertical plane as one end of the threaded rod 33. At this time, when the threaded rod 33 continues to rotate, the threaded sleeve 30 will not rotate with it.

[0043] In order to expand the stirring area of ​​the rotor 48 in the culture bottle 2 in the vertical direction, refer to Figure 3-Figure 8The circumferential outer wall of the second rotating column 34 is fixedly connected with a cam 28, the circumferential inner wall of the placing platform 12 is fixedly connected with a guide cylinder 47, the inside of the guide cylinder 47 is plugged with a push rod 37, the bottom outer wall of the guide cylinder 47 is fixedly connected with a first spring 24, the bottom end of the push rod 37 is fixedly connected with a rolling seat 25, the inner walls on both sides of the rolling seat 25 are rotatably connected with the first roller 26, the first roller 26 is in contact with the circumferential outer wall of the cam 28, the end of the first spring 24 away from the guide cylinder 47 is fixedly connected to the top outer wall of the rolling seat 25, the circumferential inner wall of the clamping frame 4 is fixedly connected with a guide column 39, the circumferential outer wall of the guide column 39 is sleeved with a sliding sleeve, one side of the sliding sleeve is fixedly connected with a second magnetic block 38, the bottom of the second magnetic block 38 is fixedly connected with a docking seat, and the top of the push rod 37 cooperates with the docking seat. , through grooves 40 are provided at the bottom of the clamping frame 4 and the top of the placing table 12. When the clamping frame 4 clamps and fixes the culture bottle 2, as the first rotating column 22 continues to rotate, the second rotating column 34 will continue to make a circular motion. When the second rotating column 34 drives the cam 28 to make a circular motion, the cam 28 will gradually lift the first roller 26 and the push rod 37 upward. As the push rod 37 is lifted upward, the second magnetic block 38 located in the clamping frame 4 can be driven to rise together. At the same time, an attraction force will be generated between the second magnetic block 38 and the rotor 48. When the rotor 48 rotates to the vicinity of the second magnetic block 38, it will be lifted up by the attraction of the second magnetic block 38, and is not limited to the stirring of the culture solution and cells at the bottom of the culture bottle 2, but the vertical stirring area of ​​the rotor 48 for the culture solution and cells in the culture bottle 2 is expanded.

[0044] In order to improve the stirring effect of the rotor 48, refer to Figure 12-13 The rotor 48 includes a spherical portion and a supporting portion. There are two groups of spherical portions, which are respectively arranged on both sides of the supporting portion. The spherical portion not only reduces the shear force on the cells during stirring, avoiding damage to the cells during stirring, but also the spherical portion can expand the area of ​​attraction between the second magnetic block 38, so that the rotor 48 can stably follow the second magnetic block 38 to rise or fall.

[0045] Working principle: When in use, the staff places the culture bottle 2 containing the culture fluid and cells on the placing table 12 and then starts the stirrer. At this time, the motor 20 drives the driving gear 19 to rotate. During the rotation of the driving gear 19, it will mesh with the driven gear 21, thereby driving the first rotating column 22 to rotate. During the rotation of the first rotating column 22, the rotating ring plate 18 and the two groups of first magnetic blocks 35 can be driven to rotate together. Through the attraction between the first magnetic block 35 and the rotor 48, the rotor 48 can make a circular motion in the culture bottle 2, realizing the stirring of the culture fluid and cells in the culture bottle 2, so that the nutrients can be diffused more quickly and evenly in the culture bottle 2, ensuring that there is always sufficient nutrition supply around the cells. At the same time, the stirring of the rotor 48 can effectively avoid the aggregation of cells in the culture bottle 2, which may cause uneven supply of nutrients and oxygen between cells and affect cell growth and function. At the same time, when the two groups of first magnetic blocks 35 follow the rotating ring plate 18 to make circular motion together, the roller will roll along the outer wall of the guide rail 42. During the rolling process of the roller, the limit frame 44, the connecting plate 43 and the first magnetic block 35 can be driven to move together. However, a limit groove 41 is provided on the top of the cross bar 36. When the first magnetic block 35 follows the movement of the roller, the rotating shaft will be restricted by the limit groove 41, thereby driving the first magnetic block 35 to reciprocate along the direction of the limit groove 41. When the two groups of magnetic blocks move toward each other, the rotation radius of the rotor 48 located in the culture bottle 2 will also be reduced accordingly. When the two groups of magnetic blocks move away from each other, the rotation radius of the rotor 48 located in the culture bottle 2 will also be increased accordingly, so that the rotation path of the rotor 48 in the culture bottle 2 is not constant, but reciprocates inward and outward, thereby effectively expanding the rotation radius of the rotor 48 in the culture bottle 2, and then more evenly stirring the culture fluid and cells in the culture bottle 2; When the first rotating column 22 rotates, the first bevel gear 31 is driven to rotate, and the mutual engagement between the first bevel gear 31 and the second bevel gear 32 can drive the second rotating column 34 and the threaded rod 33 to rotate together. During the rotation of the threaded screw, the vertical rod 7 of the threaded sleeve 30 threadedly connected to the outer wall thereof and the clamping frame 4 can move together, so that the culture bottle 2 can be quickly clamped and fixed when the stirrer is started, ensuring that the culture bottle 2 can always remain stable during the stirring operation, avoiding the situation where the culture bottle 2 is tipped over or tilted due to excessive stirring amplitude, and at the same time, the center of the culture bottle 2 is always the same as the center of the rotating ring plate 18, ensuring the stirring effect of the rotor 48 on the culture solution and cells in the culture bottle 2; After the clamping frame 4 clamps and fixes the culture bottle 2, as the first rotating column 22 continues to rotate, the second rotating column 34 will continue to make a circular motion. When the second rotating column 34 drives the cam 28 to make a circular motion, the cam 28 will gradually lift the first roller 26 and the push rod 37 upward. As the push rod 37 is lifted upward, the second magnetic block 38 located in the clamping frame 4 can be driven to rise together. At the same time, an attraction force will be generated between the second magnetic block 38 and the rotor 48. When the rotor 48 rotates to the vicinity of the second magnetic block 38, it will be lifted up by the attraction of the second magnetic block 38, and is no longer limited to the stirring of the culture fluid and cells at the bottom of the culture bottle 2, but the vertical stirring area of ​​the rotor 48 for the culture fluid and cells in the culture bottle 2 is expanded.

[0046] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A magnetically driven cell culture stirrer, comprising a housing (1), a support column (3), a locking seat (10), a transverse plate (8), a thermometer (9) and a fixing bolt (11), characterized in that: A circular groove (13) is provided on the top of the shell (1), a placement table (12) is fixedly connected to the inside of the circular groove (13), a rotating ring plate (18) is provided inside the shell (1), the rotating ring plate (18) is located below the placement table (12), and a first magnetic block (35) is provided on the top of the rotating ring plate (18), the number of the first magnetic blocks (35) is two groups, and the two groups of the first magnetic blocks (35) are symmetrically distributed; A rotating assembly for driving the two groups of the first magnetic blocks (35) to rotate is arranged inside the housing (1); A guide assembly is provided below the rotating ring plate (18) to enable the first magnetic block (35) to move horizontally when rotating; A clamping assembly for fixing the culture bottle (2) is provided above the shell (1); A rotor (48) is placed at the bottom of the culture bottle (2), and the rotor (48) and the first magnetic block (35) attract each other.

2. A magnetically driven cell culture agitator according to claim 1, characterized in that: The rotating assembly comprises a reinforcing frame (15) fixedly connected to an inner wall of one side of the housing (1); the reinforcing frame (15) is fixedly connected to a motor (20); an output end of the motor (20) is fixedly connected to a driving gear (19); a driven gear (21) is meshed on a circumferential outer wall of the driving gear (19); a first rotating column (22) is fixedly connected to a circumferential inner wall of the driven gear (21); and the rotating ring plate (18) is fixedly connected to the circumferential outer wall of the first rotating column (22).

3. A magnetically driven cell culture agitator according to claim 2, characterized in that: The guide assembly comprises a fixing plate (14) fixedly connected to the inner walls of both sides of the housing (1), one end of the fixing plate (14) being fixedly connected to a circular ring plate (17), the top outer wall of the circular ring plate (17) being fixedly connected to a connecting column (46), the top of the connecting column (46) being fixedly connected to a guide rail (42), one side of the guide rail (42) being fixedly and rollingly connected to a second roller (45), both sides of the second roller (45) being rotatably connected to a limit frame (44), the limit frame ( A notch is provided at the bottom of the first magnetic block (35), one end of the limit frame (44) is fixedly connected to a connecting plate (43), the top outer wall of the connecting plate (43) is rotatably connected to a rotating shaft, one end of the rotating shaft away from the connecting plate (43) is rotatably connected to the bottom outer wall of the first magnetic block (35), the circumferential inner wall of the rotating ring plate (18) is fixedly connected to a cross bar (36), the top outer wall of the cross bar (36) is provided with a limit groove (41), and one end of the rotating shaft passes through the inside of the limit groove (41).

4. A magnetically driven cell culture agitator according to claim 3, characterized in that: The guide rail (42) comprises a first arc-shaped portion (4201), a second arc-shaped portion (4202), and a third arc-shaped portion (4203); the number of the first arc-shaped portion (4201), the second arc-shaped portion (4202), and the third arc-shaped portion (4203) is four groups; the four groups of the first arc-shaped portion (4201), the second arc-shaped portion (4202), and the third arc-shaped portion (4203) are connected end to end to form a closed track.

5. The magnetically driven cell culture agitator according to claim 3, characterized in that: The circumferential outer wall of the first rotating column (22) is fixedly connected to a first bevel gear (31), and the circumferential outer wall of the first bevel gear (31) is meshed with a second bevel gear (32).

6. A magnetically driven cell culture agitator according to claim 5, characterized in that: The clamping assembly comprises a second rotating column (34) fixedly connected to the circumferential inner wall of the second bevel gear (32); one end of the second rotating column (34) is fixedly connected to a threaded rod (33); the circumferential outer wall of the threaded rod (33) is threadedly connected to a threaded sleeve (30); the circumferential outer wall of the threaded sleeve (30) is fixedly connected to a vertical rod (7); both sides of the vertical rod (7) extending to the outer wall outside the shell (1) are fixedly connected to arc rods (5); the arc rods (5) are fixedly connected to a clamping frame (4); two groups of the clamping frames (4) clamp and fix the culture bottle (2); the bottom inner wall of the shell (1) is fixedly connected to a fixed frame (23); the second rotating column (34) is rotatably connected to the fixed frame (23).

7. A magnetically driven cell culture agitator according to claim 6, characterized in that: A rectangular groove (6) is provided on the top outer wall of the shell (1), and one end of the vertical rod (7) passes through the inside of the rectangular groove (6).

8. The magnetically driven cell culture agitator according to claim 6, characterized in that: A vertical plate (16) is fixedly connected to the inner wall of the bottom of the housing (1), one end of a second rotating column (34) passes through one side of the vertical plate (16), a second spring (29) is fixedly connected to the outer wall of one side of the vertical plate (16), one end of the threaded sleeve (30) is fixedly connected to the circular plate (27), and one end of the threaded sleeve (30) away from the circular plate (27) is located on the same vertical plane as one end of the threaded rod (33).

9. A magnetically driven cell culture agitator according to claim 8, characterized in that: The circumferential outer wall of the second rotating column (34) is fixedly connected to a cam (28); the circumferential inner wall of the placing platform (12) is fixedly connected to a guide cylinder (47); a push rod (37) is inserted into the interior of the guide cylinder (47); the bottom outer wall of the guide cylinder (47) is fixedly connected to a first spring (24); the bottom end of the push rod (37) is fixedly connected to a rolling seat (25); the inner walls on both sides of the rolling seat (25) are rotatably connected to first rollers (26); the first rollers (26) are in contact with the circumferential outer wall of the cam (28); One end of the first spring (24) away from the guide cylinder (47) is fixedly connected to the top outer wall of the rolling seat (25); the circumferential inner wall of the clamping frame (4) is fixedly connected to a guide column (39); the circumferential outer wall of the guide column (39) is sleeved with a sliding sleeve; one side of the sliding sleeve is fixedly connected to a second magnetic block (38); the bottom of the second magnetic block (38) is fixedly connected to a docking seat; the top end of the push rod (37) cooperates with the docking seat; the bottom of the clamping frame (4) and the top of the placement table (12) are both provided with through grooves (40).

10. The magnetically driven cell culture agitator according to claim 1, characterized in that: The rotor (48) comprises a spherical portion and a supporting portion. The spherical portions are in two groups, and the two groups of spherical portions are respectively arranged on both sides of the supporting portion.

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