Portal frame and automatic extraction equipment

Abstract

The portal frame comprises a base and a moving beam, and the moving beam can move relative to the base; the moving beam comprises one or more stand columns perpendicular to the base and a cross beam perpendicular to the one or more stand columns, and the cross beam is connected with the one or more stand columns. The automatic extraction equipment comprises a sample processing device, a portal frame, a consumable component and a pipetting device. The sample treatment device comprises a uniform mixing mechanism and a magnetic suction mechanism which are respectively used for performing uniform mixing treatment and magnetic suction treatment on the biological sample. The sample processing device and the consumable component are arranged on the base. The pipetting device is arranged on the moving beam and used for achieving pipetting operation between the consumable assembly and the sample processing device.

Description

Description of the case

[0001] This application is a divisional application of the invention patent application with application number 202280021472.8, application date July 15, 2022, and name “An automatic extraction device and its use method”. Cross-references

[0002] This application claims priority to Chinese application No. 202110801973.5 filed on July 15, 2021, the entire contents of which are incorporated herein by reference. Technical Field

[0003] The present invention relates to the field of biological instrumentation, and in particular to a gantry and automatic extraction equipment. Background Art

[0004] In modern bioengineering, the extraction or purification process of target biological substances (e.g., plasmids) is usually carried out by combining manual and mechanical methods, but the manual operation accounts for a high proportion, which not only requires the selection of appropriate extraction or purification processes, but also requires a large workload and is time-consuming. At the same time, since manual operations often have individual differences, the accuracy of the results will be affected, and the consistency of process execution is not ideal. When the number of samples is large, the throughput of the test is also limited, making it difficult to meet the rapid development of current bioengineering for the extraction or purification of high-throughput samples.

[0005] Therefore, it is necessary to provide an automatic extraction device and a method for using the same, so as to achieve mechanical automation of the extraction or purification process of the target biological substance. Summary of the invention

[0006] One of the embodiments of the present specification provides a gantry, including a base and a shifting beam, wherein the shifting beam can move relative to the base; the shifting beam includes one or more columns arranged perpendicular to the base, and a cross beam perpendicular to the one or more columns, wherein the cross beam is connected to the one or more columns.

[0007] One of the embodiments of the present specification provides an automatic extraction device for extracting target biological substances, the automatic extraction device comprising: a sample processing device, a consumable component, a pipetting device and the above-mentioned gantry; the sample processing device comprises a mixing mechanism and a magnetic attraction mechanism, which are respectively used to perform mixing and magnetic attraction treatments on the biological sample; the sample processing device and the consumable component are arranged on the base of the gantry, the pipetting device is arranged on the moving beam of the gantry, and the pipetting device can be used to implement pipetting operations between the consumable component and the sample processing device. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] This specification will be further described in the form of exemplary embodiments, which will be described in detail by the accompanying drawings. These embodiments are not restrictive, and in these embodiments, the same number represents the same structure, wherein:

[0009] Figure 1 is a schematic structural diagram of a first-view automatic extraction device according to some embodiments of this specification;

[0010] Figure 2 is a schematic structural diagram of a second viewing angle of an automatic extraction device according to some embodiments of this specification;

[0011] Figure 3 is a schematic structural diagram of an automatic extraction device according to some embodiments of this specification from a third perspective;

[0012] Figure 4 is a schematic diagram of the structure of the consumable assembly according to some embodiments of this specification;

[0013] Figure 5 is a schematic diagram of the structure of the kit components according to some embodiments of this specification;

[0014] Figure 6 It is a schematic diagram of the structure of the kit according to some embodiments of this specification;

[0015] Figure 7 is a schematic diagram of the structure of a gun tip box according to some embodiments of this specification;

[0016] Figure 8 is a schematic diagram of the internal structure of a gun tip box according to some embodiments of this specification;

[0017] Fig. 9 is a schematic diagram of the structure of a liquid transfer device according to some embodiments of the present specification;

[0018] Fig.10 is a schematic diagram of the structure of a sample processing device according to some embodiments of this specification;

[0019] Fig.11 is a schematic diagram of the structure of a mixing mechanism according to some embodiments of the present specification;

[0020] Fig.12 is a schematic diagram of the structure of a tube holder according to some embodiments of this specification;

[0021] Fig.13 is a schematic diagram of the structure of the magnetic attraction mechanism shown in some embodiments of this specification;

[0022] Fig.14is a schematic diagram of the structure of the magnetic attraction mechanism shown in other embodiments of this specification;

[0023] Fig.15 is a schematic diagram of the magnet arrangement structure of the magnetic attraction mechanism shown in some embodiments of this specification;

[0024] Fig.16 It is a magnetic attraction demonstration diagram of the magnetic attraction mechanism shown in some embodiments of this specification;

[0025] Fig.17 It is a magnetic attraction demonstration diagram of the magnetic attraction mechanism shown in some embodiments of this specification;

[0026] Fig.18 It is a schematic diagram of multiple extraction channels of the automatic extraction device shown in some embodiments of the present specification.

[0027] Reference numerals: 100 - sample processing device, 110 - sample tube, 120 - reaction tube, 130 - mixing mechanism, 131 - mixing drive motor, 132 - tube holder, 1321 - notch, 133 - positioning monitoring component, 1331 - photoelectric sensor, 1332 - baffle, 140 - magnetic attraction mechanism, 141 - magnet, 1411 - magnetic attraction bracket, 142 - first magnetic attraction mechanism, 1421 - first magnet, 1421a - upper sub-magnet, 1421b - lower sub-magnet, 1422 - first magnetic attraction drive motor, 1423 - Magnetic shield, 1424-second magnetic drive motor, 1425-first magnetic bracket, 1426-two linear guide rails, 1427-screw, 143-second magnetic mechanism, 1431-second magnet, 1432-third magnetic drive motor, 1433-fourth magnetic drive motor, 1434-second magnetic bracket, 1435-guide shaft, 150-support structure, 200-gantry, 201-base, 202-transfer beam, 203-transfer beam drive motor, 204-transfer beam guide rail, 205-transfer beam lead screw nut pair, 206 - first synchronous pulley assembly, 300-consumable assembly, 301- reagent kit assembly, 3011- reagent kit, 3012- sealing layer, 3013- cavity, 302- gun tip box, 3021- gun tip, 3022- box body, 3027- cavity, 3023- cover plate, 3024- baffle, 3025- through hole, 3026- target biological material collection tube, 303- reagent kit support, 3031- handle, 304- consumable support seat, 305- consumable assembly drive motor, 306- consumable assembly guide rail, 400- pipetting device, 401-pipette, 4011-piston, 4012-pipette tip, 402-piston drive motor, 403-drive plate, 404-support plate, 405-piston guide, 406-puncture mechanism, 407-spring, 408-push plate, 409-push rod, 410-pipette drive motor, 411-pipette guide, 412-pipette screw nut pair, 413-second synchronous pulley assembly, 500-waste liquid device, 501-waste liquid box, 502-waste liquid box support, 503-waste liquid box cover plate through hole, 600-coordinate system. DETAILED DESCRIPTION

[0028] In order to more clearly illustrate the technical solutions of the embodiments of this specification, the following is a brief introduction to the drawings required for the description of the embodiments. Obviously, the drawings described below are only some examples or embodiments of this specification. For ordinary technicians in this field, this specification can also be applied to other similar scenarios based on these drawings without creative work. Unless it is obvious from the language environment or otherwise explained, the same reference numerals in the figures represent the same structure or operation.

[0029] The words "first", "second" and similar words used in the specification and claims of this application do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, words such as "one" or "an" do not indicate a quantitative limitation, but rather indicate the presence of at least one. Unless otherwise indicated, words such as "front", "rear", "lower" and / or "upper" are used for ease of description only and are not limited to one position or one spatial orientation. Generally speaking, the terms "include" and "comprise" only indicate the inclusion of clearly identified steps and elements, and these steps and elements do not constitute an exclusive list, and the method or device may also include other steps or elements.

[0030] The automatic extraction device involved in some embodiments of the present application can be used for automatic extraction and / or purification of target biological substances in biological samples, which can greatly reduce the workload of operators (e.g., experimenters, workers, etc.), shorten the working time, and adopt mechanical automation operation throughout the process, with high consistency of repetition and good accuracy of results. In some embodiments, the automatic extraction device can be used for the processing of high-throughput samples, and multiple samples can be processed at the same time, and the efficiency of extraction and / or purification is significantly improved. The target biological substance here refers to the substance to be extracted (or separated) and / or purified in the biological sample. In some embodiments, the target biological substance includes plasmids (e.g., DNA plasmids, RNA plasmids, etc.), proteins, nucleic acids, and any other feasible target biological substances. For example, the automatic extraction device can be used for automatic extraction and / or purification of plasmids. For another example, the automatic extraction device can be used for automatic extraction and / or purification of proteins. For another example, the automatic extraction device can be used for automatic extraction and / or purification of nucleic acids. In some embodiments, the biological sample can be a microorganism (including bacteria, viruses, fungi, etc., such as Escherichia coli), blood, saliva, animal tissue, plant, food, or any other feasible biological sample. It should be understood that the application scenarios of the automatic extraction equipment in the description of this application are merely some examples or embodiments of the present application. For ordinary technicians in this field, without paying any creative work, they can also apply the present application to other similar scenarios based on these drawings.

[0031] Figure 1 is a schematic structural diagram of a first-view automatic extraction device according to some embodiments of this specification; Figure 2 is a schematic structural diagram of a second viewing angle of an automatic extraction device according to some embodiments of this specification; Figure 3 is a schematic diagram of the structure of the automatic extraction device from a third perspective according to some embodiments of the specification. It should be noted that Figure 1-Figure 3 The structure of the automatic extraction device shown in the figure is for illustrative purposes only and is not intended to limit the scope of the present application.

[0032] like Figure 1 and Figure 2 As shown, the automatic extraction device includes a gantry 200, a sample processing device 100, a consumable component 300, a liquid transfer device 400 and a waste liquid device 500. It should be noted that, according to different requirements, the automatic extraction device may not be provided with the waste liquid device 500.

[0033] For ease of description, use Figure 1 The coordinate system 600 shown in the figure is used to further describe the components and / or devices of the automatic extraction device. The coordinate system 600 (e.g., a Cartesian coordinate system) includes an X-axis, a Y-axis, and a Z-axis. Among them, the Z-axis is parallel to the vertical direction (e.g., perpendicular to the ground), the X-axis and the Y-axis are parallel to the horizontal direction (e.g., parallel to the ground) and the X-axis is perpendicular to the Y-axis. The Y-axis direction is also referred to as the first direction. The X-axis direction is also referred to as the second direction. The Z-axis direction is also referred to as the third direction.

[0034] The gantry 200 is used to support one or more components and / or devices of the automatic extraction equipment (for example, the sample processing device 100, the consumable component 300, the pipetting device 400 and / or the waste liquid device 500). In some embodiments, the gantry 200 includes a base 201 and a shift beam 202. The sample processing device 100, the consumable component 300 and the waste liquid device 500 are arranged on the base 201. For example, the sample processing device 100, the consumable component 300 and the waste liquid device 500 can be arranged in sequence on the base 201 (for example, along the X-axis direction (i.e., the second direction)). The interval mentioned here refers to a certain distance apart. There is no limitation on the components and / or the arrangement order. The arrangement order can be as follows: Figure 1 As shown, the sample processing device 100 is located between the consumable assembly 300 and the pipetting device 400 and arranged on the base 201, or it can be any other arrangement order. For another example, the sample processing device 100, the consumable assembly 300 and / or the waste liquid device 500 are detachably mounted on the base 201. The pipetting device 400 is arranged on the shift beam 202. For example, the pipetting device 400 is detachably mounted on the shift beam 202. For another example, the pipetting device 400 is movably mounted on the shift beam 202. That is, the pipetting device 400 can move relative to the shift beam 202 (for example, move along the Z-axis direction) to achieve liquid suction or discharge. In some embodiments, the shift beam 202 includes a column arranged perpendicular to the base 201. For example, the base 201 is arranged parallel to the ground (for example, parallel to the XY plane), and the column is arranged perpendicular to the ground (for example, parallel to the YZ plane). In some embodiments, the shift beam 202 may be a single-column structure (a structure including a single column) or a multi-column structure (for example, a structure including multiple columns). Figure 1As shown, the shifting beam 202 may include two columns (i.e., a double-column structure), which are respectively arranged in parallel on both sides of the base 201 along the Y-axis direction (i.e., the first direction). For another example, the shifting beam 202 may include a column (i.e., a single-column structure), which is arranged on any side of the base 201 along the Y-axis direction (i.e., the first direction). The columns may include shapes such as round columns and square columns. In some embodiments, when the shifting beam 202 is a multi-column structure, in addition to two parallel columns, the shifting beam 202 is also provided with a crossbeam perpendicular to the two columns (for example, parallel to the Y-axis). The two ends of the crossbeam are respectively fixedly connected to the two columns, and such an arrangement is conducive to the stability of the shifting beam 202 structure.

[0035] In some embodiments, the shifting beam 202 is movably mounted on the base 201, that is, the shifting beam 202 can move relative to the base 201 (for example, along the X-axis direction (i.e., the second direction)). As the shifting beam 202 moves, the pipetting device 400 can move above the position where liquid needs to be aspirated or discharged. In some embodiments, the shifting beam 202 can be connected to the base 201 via a shifting beam guide rail 204. The shifting beam guide rail 204 can be a linear guide rail. The shifting beam 202 can be transmission-connected to the shifting beam drive motor 203. Driven by the shifting beam drive motor 203, the shifting beam 202 moves on the shifting beam guide rail (for example, along the X-axis direction (i.e., the second direction)). In some embodiments, the shifting beam 202 can be transmission-connected to the shifting beam drive motor 203 via a shifting beam screw nut pair 205. For example, the shifting beam 202 can be connected to the shifting beam screw nut pair 205, and the shifting beam screw nut pair 205 can be connected to the shifting beam drive motor 203 through the first synchronous pulley assembly 206. The shifting beam drive motor 203 drives the shifting beam screw nut pair 205 to rotate, thereby driving the shifting beam 202 to move along the X-axis direction (i.e., the second direction). In some embodiments, the shifting beam guide is fixedly mounted on the base 201. For example, when the shifting beam 202 is a double-beam structure, the shifting beam guides corresponding to the two columns can be fixedly mounted on both sides of the base 201 along the Y-axis direction (i.e., the first direction), so that the two columns can move on the corresponding shifting beam guides. For another example, when the shifting beam 202 is a column structure, the shifting beam guide can be fixedly mounted on the side of the base 201 corresponding to the shifting beam 202 along the Y-axis direction (i.e., the first direction). In some embodiments, the shifting beam drive motor 203 is fixedly mounted on the base 201. For example, the beam shifting driving motor 203 may be fixedly mounted at the lower portion of the base 201 (eg, close to the lower portion of any side of the base 201 along the X-axis direction (ie, the second direction)).

[0036] The consumables assembly 300 is used to carry consumables used in the biological sample processing process. The consumables may include a reagent kit, a gun tip, a target biological substance collection tube, and the like. Figure 4 Schematic diagram of the structure of the consumable components according to some embodiments of this specification. Figure 4As shown, the consumable assembly 300 may include a reagent kit assembly 301, a gun tip box 302, and a consumable support seat 304. The reagent kit assembly 301 and the gun tip box 302 are disposed on the consumable support seat 304. The reagent kit assembly 301 and the gun tip box 302 are arranged along the X-axis direction (ie, the second direction).

[0037] The kit component 301 is used to provide reagents and / or magnetic beads used in the biological sample processing process. Figure 5 Schematic diagram of the structure of the exemplary kit components according to some embodiments of this specification. Figure 5 As shown, the reagent kit assembly 301 includes a reagent kit holder 303 and one or more reagent kits 3011. The reagent kit 3011 is placed on the consumables support 304 through the reagent kit holder 303. Before the biological sample is processed, one or more reagent kits can be loaded onto the reagent kit holder 303 as required by the biological sample processing process, so as to add the reagents and / or magnetic beads required for processing to the biological sample during the biological sample processing process.

[0038] The reagent box support 303 is used to load the reagent box 3011. One or more reagent boxes 3011 can be loaded on the reagent box support 303. For example, one or more reagent boxes 3011 can be installed on the reagent box support 303 by means of snap connection or limiting. In some embodiments, one or more reagent box support moving parts (e.g., handles 3031) are provided on the reagent box support 303 to move the reagent box support 303. For example, the operator can hold the reagent box support 303 by the handle 3031. Before the biological sample is processed, the operator takes the reagent box support 303 out of the consumables support 304 by holding the handle 3031, and then the operator loads the reagent box 3011 into the reagent box support 303, and then the operator installs the reagent box support 303 loaded with the reagent box 3011 onto the consumables support 304. The reagent kit support 303 facilitates the installation of multiple reagent kits 3011 as a whole into the consumable assembly 300, and also facilitates the unloading of multiple reagent kits 3011 from the consumable assembly 300 and / or the replacement of reagent kits 3011 after the use of the automatic extraction device. The reagent kit support moving member (e.g., handle 3031) makes the operation of moving the reagent kit support 303 more convenient.

[0039] The reagent box 3011 may include one or more cavities. When the reagent box 3011 includes multiple cavities, the multiple cavities may be arranged in one or more rows (e.g., 1 row, 2 rows, 3 rows, etc.) along the Y-axis direction (i.e., the first direction). Each row of cavities extends along the X-axis direction (or the second direction). Arranging the multiple cavities in multiple rows can avoid the reagent box 3011 being too long along the X-axis due to too many required cavities, thereby avoiding the overall length of the automatic extraction device along the X-axis direction (or the second direction) being too long. Figure 6Schematic diagram of the structure of the kit according to some embodiments of this specification. Figure 6 As shown, the reagent kit 3011 includes a plurality of cavities 3013 arranged in two rows.

[0040] In some embodiments, the capacity of multiple cavities 3013 may be the same or different. For example, the cavity 3031 may be determined according to the amount of reagents to be contained. The cavity 3013 of the test kit is used to contain reagents and / or magnetic beads required for the biological sample processing process. Each cavity 3013 may contain the same or different reagents and / or magnetic beads. For example, the corresponding type of reagents and / or magnetic beads may be selected according to the target biological substance to be extracted and / or the different processing procedures. The magnetic beads described herein are a kind of magnetic particles having surface active groups. Under certain conditions, the surface active groups of the magnetic beads can be combined (for example, coupled) with the target biological substance (for example, the substance to be extracted) or impurities (for example, other substances except the substance to be extracted) in the biological sample, and the magnetic beads can be magnetically attracted under the action of the external magnetic field, thereby achieving separation of active substances and impurities and / or purification of active substances. In some embodiments, the corresponding type of magnetic beads may be selected in combination with different particle sizes, different dispersities, different magnetic response times, and different coating matrices of the magnetic beads, and the types of magnetic beads are not limited here. The types of reagents may include solvents for reacting with biological samples, for washing magnetic bead conjugates, and for eluting magnetic bead conjugates to separate magnetic beads, etc. The types of reagents are not limited here. Taking the extraction of plasmids from bacterial mud as an example, the reagents may include bacterial mud lysis solution, washing solution, elution solution, etc. Magnetic beads may include magnetic beads that can bind to impurities after bacterial mud lysis, magnetic beads that can bind to plasmids, etc.

[0041] In some embodiments, the reagent box 3011 further includes a sealing layer. The sealing layer can be used to seal the cavity 3013 to prevent the reagent in the cavity from spilling and / or deteriorating (e.g., reacting with gas in the air) when the reagent box 3011 is removed. In some embodiments, one reagent box 3011 can correspond to one or more sealing layers. For example, the sealing layer can be as follows: Figure 6 The sealing layer 3012 shown in the figure can be used to seal multiple cavities 3013 of a reagent box 3011. For another example, the sealing layer can correspond to the cavities of the reagent box 3011 one by one, and the multiple cavities of the reagent box 3011 are sealed with separate sealing layers. In some embodiments, the sealing layer can be in the form of a sealing film, and the sealing film can be an aluminum film or a plastic film, etc., and the material of the sealing film is not limited here.

[0042] The gun tip box 302 is used to provide gun tips and / or target biological material collection tubes required for pipetting during biological sample processing. In some embodiments, the gun tip box 302 corresponds to the reagent box 3011 one-to-one. That is, one reagent box 3011 corresponds to one gun tip box 302. By having a one-to-one correspondence between the gun tip box 302 and the reagent box 3011, when there are multiple reagent boxes 3011, it is possible to simultaneously perform pipetting operations on multiple reagent box devices 3011, simplify the operation of the automatic extraction device, and reduce the time consumption of the automatic extraction process. Figure 7 Schematic diagram of the structure of the gun tip box according to some embodiments of this specification. Figure 7 As shown, the gun tip box assembly includes one or more gun tips 3021, a box body 3022 with an upper opening, a cover plate 3023, and one or more target biological substance collection tubes 3026. The cover plate 3023 is connected to the opening of the box body 3022. The gun tip 3021 and the target biological substance collection tube 3026 are loaded on the cover plate 3023 and the box body 3022 through the through hole 3025 provided on the cover plate. In some embodiments, the size of the through hole 3025 can be set to allow the gun tip 3021 to be placed vertically or nearly vertically, so as to facilitate the cooperation between the tail of the gun tip 3021 and the pipetting device 400. Before the biological sample is processed, one or more gun tips 3021 and the target biological substance collection tube 3026 can be loaded on the cover plate 3023 and the box body 3022 as needed in the biological sample processing process, so as to add / remove the reagents, magnetic beads and / or waste liquid required for processing to the biological sample during the biological sample processing process.

[0043] The box body 3022 and the cover plate 3023 are used to load one or more gun tips 3021 and a target biological substance collection tube 3026. The box body 3022 is divided into multiple cavities by barriers, and the cover plate 3023 is provided with multiple through holes 3025 corresponding to the multiple cavities. For example, a single through hole 3025 corresponds to the opening of a single cavity. At least part of the cavities and their corresponding through holes 3025 are used to load one or more gun tips 3021. In some embodiments, the multiple cavities of the box body 3022 can be arranged in one or more columns (for example, 1 column, 2 columns, 3 columns, etc.) along the Y-axis direction (i.e., the first direction). Each column of cavities extends along the X-axis direction (i.e., the second direction). Figure 8 Schematic diagram of the internal structure of the gun tip box according to some embodiments of this specification. Figure 7 and Figure 8 As shown, the box body 3022 includes a plurality of baffles 3024 to divide the box body 3022 into a plurality of cavities 3027. The plurality of cavities 3027 are arranged in two rows along the Y-axis direction (i.e., the first direction). By loading a gun tip 3021 in a cavity 3027, it is possible to avoid the gun tip that has pipetted different reagents to generate aerosols and other contamination in the box body 3022, which may affect the biological sample processing process.

[0044] The gun tip 3021 is used to absorb different reagents, magnetic beads and / or waste liquids in combination with the pipetting device 400 during the biological sample processing process. Multiple gun tips 3021 can be arranged in one or more rows along the Y-axis direction (i.e., the first direction). Each row of gun tips extends along the Y-axis direction (i.e., the first direction). In some embodiments, the number of columns of multiple gun tips 3021 and the number of columns of the cavities 3013 of the corresponding reagent box 3011 can be the same or different. For example, multiple gun tips 3021 are arranged in two rows, and the corresponding cavities 3013 of the reagent box 3011 are arranged in three rows. For another example, multiple gun tips 3021 and the corresponding cavities 3013 of the reagent box 3011 are both arranged in three rows. In some embodiments, the gun tips 3021 are loaded into the corresponding cavities 3027 through the through holes 3025 of the cover plate 3023. In combination Figure 7 and Figure 8 As shown, the head of the gun tip 3021 for aspirating liquid passes through the through hole 3025 and is placed in the cavity 3027 corresponding to the through hole 3025, and the tail of the gun tip 3021 that cooperates with the liquid transfer device 400 is placed outside the cavity 3027. In some embodiments, multiple gun tips 3021 may be identical or not identical. For example, the size of the gun tip 3021 may be set according to the amount of liquid that the gun tip 3021 needs to transfer. For another example, gun tips 3021 with different structures may be used to adapt to different reagents, which is conducive to the smooth liquid extraction of each reagent. In some embodiments, during the biological sample processing process, a single gun tip 3021 may be used once or repeatedly. For example, for two stages in which the same reagent needs to be transferred from the reagent box 3011 during the biological sample processing process, the same gun tip 3021 may be reused to transfer the same reagent. That is, the gun tip 3021 may not be set one-to-one with the cavity 3013 of the reagent box 3011, and the cavity 3013 containing the same reagent may correspond to the same gun tip 3021. For another example, in order to make the reagent amount more accurate or avoid mutual contamination, each gun tip 3021 is used to transfer liquid only once. That is, the gun tip 3021 can be set in a one-to-one correspondence with the cavity 3013 of the reagent box 3011, so that different gun tips 3021 are used to absorb reagents from different cavities 3013.

[0045] The target biological substance collection tube 3026 is used to load the target biological substance extracted, separated and / or purified during the biological sample processing. In some embodiments, the gun tip box 302 may include one or more target biological substance collection tubes 3026. For example, the number and / or size of the target biological substance collection tubes may be determined based on the amount of target biological substance that can be collected. In some embodiments, the target biological substance collection tube 3026 is loaded into the corresponding cavity 3027 through the through hole 3025 of the cover plate 3023. Figure 7As shown, at least a portion of the target biological substance collection tube 3026 is located above the corresponding through hole 3025 to facilitate the removal of the target biological substance into the target biological substance collection tube 3026 .

[0046] In some embodiments, the consumable assembly 300 may further include a consumable assembly drive motor. The consumable assembly drive motor 305 is used to drive the consumable assembly 300 to move along the Y-axis direction (i.e., the first direction) to adjust the position of the consumable assembly 300, so as to facilitate the pipetting operation. For example, when the number of columns of the test kit 3011 and the gun tip box 302 is inconsistent, the pipetting can be realized by multiple movements of the entire consumable assembly 300, such as first moving the consumable assembly 300 to the position where the pipetting device 400 can take the gun tip 3021 to assemble the gun tip 3021, and then moving the consumable assembly 300 and the pipetting device 400 so that the assembled gun tip 3021 can remove reagents from the test kit 3011. In some embodiments, the consumable assembly drive motor 305 is connected to the consumable support seat 304 in transmission. In some embodiments, the consumable assembly drive motor 305 can be a screw motor. For example, the consumable assembly drive motor 305 can be connected to the consumable assembly drive motor 305 through the consumable assembly screw nut pair (similar to the beam moving screw nut pair), and the consumable assembly drive motor 305 drives the consumable assembly screw nut pair to rotate, thereby driving the consumable assembly 300 to move along the Y-axis direction (i.e., the first direction). In some embodiments, the consumable assembly drive motor 305 can be mounted and fixed on the base 201. In some embodiments, the consumable assembly 300 may include a plurality of consumable assembly drive motors. For example, the consumable assembly drive motor 305 may include a first consumable assembly drive motor and a second consumable assembly drive motor. The first consumable assembly drive motor is used to drive the reagent kit assembly 301 to move along the Y-axis (i.e., the first direction). The second consumable assembly drive motor is used to drive the gun tip box 302 to move along the Y-axis (i.e., the first direction). Through multiple consumable assembly drive motors, when the number of columns of the reagent kit 3011 and the gun tip box 302 is inconsistent, the gun tip box 302 and / or the reagent kit assembly 301 can be moved separately to make the pipetting operation more efficient. For example, when the number of columns of the reagent box 3011 and the gun tip box 302 are inconsistent, the pipetting device 400 and the gun tip box 302 can be moved first to assemble the gun tip 3021, and then the reagent box assembly 301 and the pipetting device 400 can be moved so that the assembled gun tip 3021 can transfer the reagent from the reagent box 3011. For another example, when the number of columns of the reagent box 3011 and the gun tip box 302 are inconsistent, the gun tip box 302 and the reagent box assembly 301 can be moved at the same time, so that after the pipetting device 400 is assembled with the gun tip 3021, it can transfer the reagent from the reagent box 3011 after moving along the X axis.

[0047] In some embodiments, the consumable support seat 304 can be connected to the base 201 via a consumable assembly rail 306. In some embodiments, the consumable assembly rail 306 can be a linear rail. The consumable assembly rail extends along the Y-axis direction (i.e., the first direction). The consumable assembly 300 can move on the consumable assembly rail under the drive of the consumable assembly drive motor. In some embodiments, the consumable assembly rail (e.g., the second linear guide 306) is located at the lower part of the consumable support seat 304. In some embodiments, the consumable assembly rail (e.g., the second linear guide 306) can be mounted and fixed on the base 201. In some embodiments, the consumable assembly 300 may include a plurality of consumable assembly rails (e.g., Figure 4 Two second linear guide rails 306 are shown. Through multiple consumable assembly rails, the consumable assembly 300 can be stably moved without deviating from the preset direction. In some embodiments, when the consumable assembly 300 includes multiple consumable assembly drive motors, each consumable assembly drive motor can correspond to one or more consumable assembly guide rails.

[0048] The liquid transfer device 400 is used to implement a liquid transfer operation in a biological sample processing process. For example, the liquid transfer device 400 can be used for a liquid transfer operation between the consumable component 300, the sample processing device 100 and / or the waste liquid device 500. Fig. 9 Schematic diagram of the structure of the liquid transfer device according to some embodiments of this specification. Fig. 9 As shown, the pipetting device 400 includes one or more pipettes 401. The one or more pipettes 401 are arranged along the Y-axis direction (ie, the first direction).

[0049] The pipette 401 can be detachably sealed and connected to the gun tip 3021 in the gun tip box 302, so as to realize the replacement of the gun tip 3021 by the pipette 401. When the pipette 401 is sealed and connected to the gun tip 3021, the pipette 401 can realize liquid suction and liquid discharge. After the pipette 401 finishes discharging, the gun tip 3021 sealed and connected thereto can be disassembled and placed in the cavity 3027 corresponding to the gun tip. In some embodiments, the structure of the pipette 401 can be a pipette gun structure, a syringe structure or other structure that can perform liquid suction and liquid discharge operations. In some embodiments, the pipette 401 includes a cavity and a piston 4011. The cavity can be detachably connected to the gun tip 3021. For example, the cavity has a suction tip 4012, and the pipette 401 is detachably sealed and connected to the gun tip 3021 through the suction tip 4012. In some embodiments, the suction tip 4012 is aligned with a row of gun tips 3021 extending along the X-axis direction (i.e., the second direction) so that the suction tip 4012 and the gun tips 3021 are detachably sealed and connected. The first end (e.g., the lower end) of the piston 4011 can seal the cavity. The piston 4011 can move up and down relative to the cavity (e.g., move up and down parallel to the Z-axis direction (i.e., the third direction)). When the piston 4011 moves upward along the Z-axis direction (i.e., the third direction), the pipette 401 can control the gun tip 3021 to absorb liquid. When the piston moves downward along the Z-axis direction (i.e., the third direction), the pipette 401 can control the gun tip 3021 to discharge liquid.

[0050] In some embodiments, the pipetting device 400 further includes a piston drive assembly. For example, the piston drive assembly includes a piston drive motor 402, a drive plate 403, a piston guide rail 405, etc. or any combination thereof. The piston drive motor 402 is used to drive the piston 4011 to move up and down parallel to the Z-axis direction (i.e., the third direction). In some embodiments, the piston drive motor 402 can be a screw motor. The drive plate 403 is used to be fixedly connected to the second end (e.g., the upper end) of the piston 4011. The drive plate 403 is connected to the piston drive motor 402 through a transmission connection, and the piston drive motor 402 can drive the drive plate 403 to move along the Z-axis direction (i.e., the third direction), thereby driving the piston 4011 to move along the Z-axis direction (i.e., the third direction). In some embodiments, the pipetting device 400 can further include a support plate 404. The support plate 404 is used to install and fix one or more components (e.g., a cavity, a piston drive motor 402, a piston guide rail 405, etc.) of the pipetting device 400. For example, the piston guide rail 405 can be arranged on the support plate 404 along the Z-axis direction (i.e., the third direction), and the driving plate 403 is connected to the piston guide rail 405. The driving plate 403 can move on the piston guide rail 405 under the drive of the piston driving motor 402, thereby driving the piston 4011 to move up and down to achieve liquid suction and discharge. In some embodiments, the piston guide rail 405 may include a linear guide rail.

[0051] In some embodiments, the pipetting device 400 further includes a puncturing mechanism 406. The puncturing mechanism 406 is used to puncture the sealing layer (e.g., the sealing layer 3012) of the reagent kit 3011, so that the gun tip 3021 sealed with the pipette 401 can pass through the sealing layer to absorb the reagent in the reagent kit 3011. The puncturing mechanism 406 corresponds to the pipette 401 one by one. For example, one puncturing mechanism 406 corresponds to one pipette 401. In some embodiments, the puncturing mechanism 406 includes a sharp head for puncturing the sealing layer. For example, the puncturing mechanism 406 can be a magnetic film knife structure. In some embodiments, the pipetting device 400 can further include a push plate 408 and a push rod 409. The push rod 409 is connected to the push plate 408, and a spring 407 is sleeved on the push rod 409. Before the pipette 401 absorbs the reagent from the reagent box 301, when the driving plate 403 pushes the push rod 409 to move downward parallel to the Z-axis direction (i.e., the third direction), the push rod 409 pushes the push plate 408 to move downward and compresses the spring 407. When the driving plate 403 moves away from the push rod, the compressed spring 407 restores the elastic force, and the push plate 408 is reset by the restoring force of the spring 407. In some embodiments, the push plate 408 is located above the suction head 4012 and the piercing mechanism 406. The piercing mechanism 406 and the suction head 4021 are arranged along the X-axis direction (i.e., the second direction) or the Y-axis direction (i.e., the first direction). When the driving plate 403 pushes the push rod 409 to move downward, the push rod 409 pushes the push plate 408 to move downward, and the suction head 4021 of the pipette 401 can contact with the gun head 3021 and seal the connection or the piercing mechanism 406 pierces the sealing layer of the reagent box 3011.

[0052] In some embodiments, the pipetting device 400 may further include a pipetting device drive assembly. The pipetting device drive assembly includes a pipetting device drive motor 410, a pipetting device guide rail 411, etc. or any combination thereof. The pipetting device drive motor may be connected to the support plate 404 in a transmission manner. Driven by the pipetting device drive motor 410, the support plate 404 may move up and down relative to the shift beam 202 along the Z-axis direction (i.e., movement in the third direction), so that the pipette 401 as a whole can move up and down along the Z-axis direction (i.e., the third direction). In some embodiments, the pipetting device drive motor 410 may be a screw motor. In this case, the pipetting device 400 also includes a pipette screw nut pair 412 and a second synchronous pulley assembly 413. The pipette screw nut pair 412 may be connected to the pipetting device 400 (e.g., support plate 404). The pipette screw nut pair 412 can be connected to the pipette drive motor 410 through the second synchronous pulley assembly 413, and the pipette drive motor 410 drives the pipette screw nut pair 412 to rotate to drive the support plate 404 to move along the Z-axis direction (i.e., the third direction), so that the pipette 401 can move up and down along the Z-axis direction (i.e., the third direction). In some embodiments, the pipette 400 also includes a pipette guide rail 411. The pipette guide rail 411 can be a linear guide rail. The pipette guide rail 411 can be installed and fixed on the shift beam 202 along the Z-axis direction (i.e., the third direction). The support plate 404 can be connected to the shift beam 202 through the pipette guide rail 411. Under the drive of the pipette drive motor 410, the support plate 404 can move up and down along the pipette guide rail 411, thereby driving the pipette 401 to move up and down along the Z-axis direction (i.e., the third direction).

[0053] The waste liquid device 500 is used to hold the waste liquid generated during the biological sample processing process. The waste liquid device 500 may include a waste liquid box 501 and a waste liquid box support 502. The waste liquid box 501 is detachably mounted on the waste liquid box support 502. The waste liquid box 501 is relatively fixed to the base 201. For example, the waste liquid box 501 can be mounted on the sample processing device 100 through the waste liquid box support 502. In some embodiments, the waste liquid box 501 may include a waste liquid box body and a waste liquid box cover. The waste liquid box body is open at the upper end. One or more through holes 503 are provided on the waste liquid box cover to facilitate the transfer of waste liquid into the waste liquid box 501. The one or more through holes 503 correspond to the sample tubes 110 and / or the reaction tubes 120 in the sample processing device 100. For example, if the sample processing device 100 includes 6 sample tubes, the waste liquid box cover includes 6 through holes. In some embodiments, the waste liquid box 501 may include multiple waste liquid box bodies and corresponding multiple waste liquid box covers. The waste liquid box bodies correspond to the sample tubes 110 and / or reaction tubes 120 in the sample processing devices 100 one by one. The waste liquid box covers are respectively provided with a through hole. For example, if the sample processing device 100 includes 6 sample tubes, the waste liquid box 501 includes 6 waste liquid box bodies corresponding to the 6 sample tubes. In some embodiments, the waste liquid box 501 may not include a waste liquid box cover.

[0054] The sample processing device 100 is used to perform mixing and magnetic treatment on biological samples. In some embodiments, the sample processing device 100 may include a mixing mechanism and a magnetic attraction mechanism. The mixing mechanism is used to mix the biological samples during the treatment process. The magnetic attraction mechanism is used to perform magnetic treatment on the biological samples during the treatment process. In some embodiments, the sample processing device 100 may also include a sample tube and a reaction tube, the sample tube can be used to hold the biological sample, and the reaction tube can be used to hold the solution obtained by the sample tube treatment. In some embodiments, the capacity of the sample tube can be equal to or greater than the capacity of the reaction tube. In some embodiments, the sample tube can be the same height as the reaction tube. In some embodiments, the sample tube and / or the reaction tube can be in the shape of a test tube, or can be a box structure with an open top, which is not limited here.

[0055] Fig.10 It is a schematic diagram of the structure of a sample processing device according to some embodiments of the present specification. Fig.10 It is a schematic diagram of the structure of the mixing mechanism shown in some embodiments of this specification. Fig.12 It is a schematic diagram of the structure of the tube holder shown in some embodiments of this specification. Fig.13 It is a schematic diagram of the structure of the magnetic attraction mechanism shown in some embodiments of this specification. Fig.14 It is a structural schematic diagram of the magnetic attraction mechanism shown in other embodiments of this specification. Fig.15It is a schematic diagram of the magnet arrangement structure of the magnetic attraction mechanism shown in some embodiments of this specification. Fig.16 It is a magnetic attraction demonstration diagram of the magnetic attraction mechanism shown in some embodiments of this specification. Fig.17 It is a magnetic attraction demonstration diagram of the magnetic attraction mechanism shown in some embodiments of this specification.

[0056] The sample processing device 100 may include a mixing mechanism 130 and a magnetic attraction mechanism 140. The sample processing device 100 may also include at least one of a sample tube 110 and a reaction tube 120.

[0057] The sample tube 110 is a container for accommodating biological samples, pre-treating biological samples and / or reacting biological samples. The reaction tube 120 is a container for accommodating biological samples, pre-treating biological samples and / or reacting biological samples. In some embodiments, the sample processing device 100 may include only the sample tube 110, that is, only the sample tube 110 is set for sample processing. In some embodiments, the sample processing device 100 may include only the reaction tube 120, that is, only the reaction tube 120 is set for sample processing. In some embodiments, the sample processing device 100 may include the sample tube 110 and the reaction tube 120, and both of them jointly undertake the sample processing work. In some embodiments, the biological sample pretreatment may include biological culture, resuspension, magnetic adsorption, separation or any other feasible biological pretreatment operation. In some embodiments, the biological sample reaction may include resuspension, magnetic adsorption, separation, lysis, precipitation or other feasible biological sample reaction operations. In some embodiments, the sample tube 110 can be used to accommodate biological samples to be reacted, for example, the sample tube 110 can be used to accommodate biological samples to be placed in the reaction tube 120 for reaction. In some embodiments, the reaction tube 120 can be used to accommodate the biological sample transferred from the sample tube 110 . For example, the reaction tube 120 can be used to accommodate the biological sample transferred from the sample tube 110 that has undergone biological sample pretreatment.

[0058] In some embodiments, the structure and / or capacity of the sample tube 110 and the reaction tube 120 may be the same. For example, the tube body shapes of the sample tube 110 and the reaction tube 120 are both straight tubes. For another example, the capacities of the sample tube 110 and the reaction tube 120 are both 100 ml. In some embodiments, the structure and / or capacity of the sample tube 110 and the reaction tube 120 may be different. In a specific embodiment, the structural form, capacity and other specification parameters of the sample tube 110 or the reaction tube 120 may be set in any feasible manner, and the embodiments of this specification do not particularly limit them.

[0059] In some embodiments, the sample processing device 100 may include a sample tube 110 and a reaction tube 120. In some embodiments, the sample processing device 100 may include a sample tube 110 and multiple reaction tubes 120. In some embodiments, the sample processing device 100 may include multiple sample tubes 110 and multiple reaction tubes 120, so as to realize the processing of multiple samples and improve the efficiency of biological sample processing. In some embodiments, the number of sample tubes 110 or reaction tubes 120 may be 6, 8 or other numbers. In some embodiments, the sample processing device 100 may be provided with multiple processing channels arranged at intervals, and each processing channel may include a sample tube 110 and at least one reaction tube 120, that is, the number of reaction tubes 120 may be one or more (at least two). Here, each processing channel may be understood as a unit sample processing unit within a sample processing system, because usually each unit sample processing unit has a sample tube 110 and at least one reaction tube 120, which can meet a certain category or a certain sample processing flow. For more descriptions of processing channels, please refer to Fig.18 and its description.

[0060] When there are multiple sample tubes 110 and multiple reaction tubes 120, the channel setting method according to different processing channels not only expands the sample processing scale, but also facilitates the individual management of each sample processing channel. For example, each processing channel can be set differently. In some embodiments, the differentiated setting of the processing channel can be determined according to the characteristics of the sample processing process. For example, the sample processing process characteristics can include the required number of reaction tubes 120, the required capacity of sample tubes 110 or reaction tubes, etc. The sample processing process characteristics can be determined based on the sample processing volume (for example, a larger sample processing volume exceeding a certain threshold, or a smaller sample processing volume below a certain threshold), the sample processing type (for example, a plasmid purification process or a protein extraction process) or any other feasible sample processing parameters.

[0061] In some embodiments, each processing channel can be arranged in parallel with each other. In some embodiments, each processing channel can be arranged longitudinally or transversely. In some embodiments, the sample tube 110 and the reaction tube 120 of each processing channel can be arranged in parallel in sequence along (exemplarily) the X-axis direction (i.e., the second direction), exemplarily, as shown in FIG. Fig. 9 As shown, the sample processing device 100 includes 6 channels, and the sample tubes 110 and reaction tubes 120 in each channel are arranged in parallel at a certain interval along the X-axis direction (i.e., the second direction), and multiple channels are arranged in parallel along the Y-axis direction (i.e., the first direction). In some embodiments, a predetermined interval is set between the sample tubes 110 and the reaction tubes 120.

[0062] The mixing mechanism 130 is used to mix the biological sample in the sample tube 110 and / or the biological sample in the reaction tube 120, so as to achieve a mixing effect of the biological samples. In some embodiments, the mixing mechanism 130 can be used alone to mix the biological sample in the sample tube 110. In some embodiments, the mixing mechanism 130 can be used alone to mix the biological sample in the reaction tube 120. In some embodiments, the mixing mechanism 130 can be used to mix the biological sample in the sample tube 110 and the biological sample in the reaction tube 120 simultaneously or sequentially.

[0063] In some embodiments, the mixing mechanism 130 may be driven by a motor (e.g., an electric drive or a shaker electromagnetic drive, etc.) or any other feasible drive mode (e.g., a hydraulic drive, a pneumatic drive, etc.). In some embodiments, the mixing mechanism 130 may include a mixing drive motor 131 and a tube holder 132, wherein the mixing drive motor 131 is in transmission connection with the tube holder 132, wherein the sample tube 110 and / or the reaction tube 120 are disposed in the tube holder 132, and the mixing drive motor 131 can drive the tube holder 132 to move, thereby driving the biological sample in the sample tube 110 and / or the biological sample in the reaction tube 120 to be mixed.

[0064] The tube holder 132 is used to place the sample tube 110 and / or the reaction tube 120, and can drive the sample tube 110 and / or the reaction tube 120 to move. In some embodiments, the tube holder 132 can be a cylindrical structure that is sleeved with the internal sample tube 110 or the reaction tube 120. In some embodiments, the sample tube 110 or the reaction tube 120 can be freely sleeved on the tube holder 132, or can be freely taken out of the tube holder 132. In some embodiments, the sample tube 110 or the reaction tube 120 and the tube holder 132 can be installed in a fixed connection manner. In some embodiments, the sample tube 110 or the reaction tube 120 and the tube holder 132 are detachably connected.

[0065] In some embodiments, the tube holder 132 is connected to the rotating shaft (not shown in the drawings) of the mixing drive motor 131. Under the action of the rotating shaft, the mixing drive motor 131 can drive the tube holder 132 to rotate axially, so that the tube holder 132 drives the sample tube 110 and / or the reaction tube 120 to rotate axially to achieve mixing treatment of the biological sample. This axial rotation driving mode can achieve full mixing of the biological sample in the sample tube 110 and / or the reaction tube 120, thereby improving the mixing treatment efficiency. In some embodiments, the driving rotation mode of the mixing drive motor 131 and the tube holder 132 can also be set to any other feasible structural mode. For example, the mixing drive motor 131 can drive the tube holder 132 to rotate at multiple angles (such as 360 degrees, etc.) around the center line of the tube holder 132, and can also drive the tube holder 132 to rotate at a constant or variable inclination angle in a corresponding plane (such as a horizontal plane, etc.). In some embodiments, the mixing drive motor 131 can drive the tube holder 132 to rotate in one axial direction for a period of time and then rotate in the opposite axial direction (for example, first rotate in a clockwise axial direction and then rotate in a counterclockwise axial direction), so that the tube holder 132 drives the sample tube 110 and / or the reaction tube 120 to rotate in an axially changing direction. This axially changing direction rotation setting mode can ensure that the biological samples in the sample tube 110 and / or the reaction tube 120 are fully mixed and improve the mixing processing efficiency. In some embodiments, the rotation speed of the mixing drive motor 131 can be set differently according to the specific situation, so as to drive the tube holder 132 to rotate at different speeds (for example, high speed, slow speed, etc.), for example, it can be set to a rotation speed of 1500r / min.

[0066] In some embodiments, the mixing mechanism 130 may include multiple mixing drive motors 131 and multiple tube holders 132, and the multiple mixing drive motors 131 are connected to the multiple tube holders 132 in a one-to-one transmission manner, and each tube holder 132 is provided with a sample tube 110 or a reaction tube 120. This setting method can realize the driving of sample tubes or reaction tubes in multiple tube holders in multiple transformation modes, and different mixing drive motors 131 can be set to drive different drive modes of corresponding tube holders 132 respectively. Among them, the driving mode can be determined by driving parameters such as driving force parameters, driving start time, start stop time, etc. It is also possible to realize the specific driving mode setting of some mixing drive motors 131 in the multiple mixing drive motors 131, especially the sample processing scene of multiple processing channels, so as to better realize the targeted setting applicable to a variety of different mixing processing conditions, so that the mixing process of the sample processing process is more efficient and meets the diversified sample processing requirements. In some embodiments, each mixing drive motor 131 can be controlled separately to drive the corresponding tube holder 132 to move, so as to facilitate independent control and meet personalized mixing requirements.

[0067] The magnetic attraction mechanism 140 is used to achieve magnetic attraction of the biological sample in the sample tube 110 and / or the biological sample in the reaction tube 120 to facilitate the biological separation operation. The magnetic attraction mechanism 140 may include a magnet 141, which can move relative to the sample tube 110 and / or the reaction tube 120 to perform magnetic attraction treatment on the biological sample in the sample tube 110 and / or the biological sample in the reaction tube 120. In some embodiments, the magnet 141 has a predetermined magnetic attraction area. In some embodiments, the magnetic attraction area of ​​the magnet 141 may be the area of ​​the magnetic attraction surface facing the sample tube 110 and / or the reaction tube 120 for magnetic attraction treatment.

[0068] In some embodiments, for a single processing channel, a magnet 141 may be disposed on the other side of the sample tube 110 away from the reaction tube 120 along the X-axis direction. In some embodiments, for each processing channel, a magnet 141 may be disposed between two sample tubes 110 along the Y-axis direction. In some embodiments, for multiple processing channels, a magnet 141 may be disposed at any outermost side of the sample tubes 110 at both ends along the Y-axis direction. In some embodiments, for a single processing channel, one magnet 141 or two corresponding magnets 141 may be disposed between adjacent sample tubes 110 and reaction tubes 120, or between two adjacent reaction tubes 120. In some embodiments, the aforementioned two corresponding magnets 141 may be interspersed between adjacent sample tubes 110 and reaction tubes 120, or between two adjacent reaction tubes 120. In some embodiments, the magnet 141 may be disposed on a magnetic support 1411 of the magnetic attraction mechanism 140, and the magnetic support 1411 may be any feasible support structure as long as it can carry the magnet 141. As an example only, Fig.14 The figure shows several layouts of the magnetic attraction mechanism 140, namely (A), (B), (C), (D), and (E). Specifically, the two magnets 141 may be arranged on one side of two tubes (sample tube 110 and reaction tube 120) and spaced apart as shown in (A), or the two magnets 141 may be arranged adjacent to each other between the two tubes as shown in (B), or the two magnets 141 in (B) may be arranged on opposite sides of the magnetic attraction bracket 1411 as shown in (C), or multiple magnets 141 may be arranged between the two tubes and on the outside of each tube, or a multi-channel array arrangement may be shown in (E). It should be noted that the magnetic attraction mechanism 140 may also adopt any other feasible method as long as it can achieve its magnetic attraction processing of the sample tube 110 and / or the reaction tube 120.

[0069] In some embodiments, the side wall of the tube holder 132 may be provided with at least one notch 1321, and the mixing mechanism 130 may further include a positioning monitoring component 133, which is used to monitor the rotation position of the tube holder 132, so that when the tube holder 132 stops rotating, at least one notch 1321 faces the magnet 141, so as to perform magnetic attraction processing on the biological sample in the sample tube 110 and / or the reaction tube 120. By setting the matching structure of the notch 1321 of the tube holder 132 and the positioning monitoring component 133 in the mixing mechanism 130, it is convenient to monitor the rotation position of the notch 1321 during the mixing process, so as to control the mixing mechanism 130 to stop rotating when the notch 1321 rotates to a position close to the magnet 141. This setting satisfies the magnetic attraction positioning control in various mixing process control processes, ensures the accuracy of magnetic attraction positioning, and thus improves the mixing process efficiency and magnetic attraction process efficiency of the sample processing device and its automatic extraction device.

[0070] In some embodiments, the corresponding tube holder 132 of the sample tube 110 and / or the reaction tube 120 may be provided with a notch on one side of the tube holder 132, as long as it can realize the magnetic suction processing of the sample tube 110 and / or the reaction tube 120, and the embodiment of the present application does not specifically limit its opening position. In some embodiments, in order to realize the multi-directional and convenient magnetic suction processing operation of the sample tube 110 and / or the reaction tube 120, the corresponding tube holder 132 of the sample tube 110 and / or the reaction tube 120 may be provided with at least two notches 1321 on at least two sides of the tube holder 132, for example, one notch 1321 is respectively provided on two opposite sides of the tube holder 132. In some embodiments, the opening area of ​​the notch 1321 may be larger than the magnetic suction action area of ​​the magnetic suction mechanism 140, for example, the opening area of ​​the notch 1321 may be larger than the area of ​​the magnetic suction surface of the magnet 141 facing the notch 1321.

[0071] In some embodiments, the positioning monitoring component 133 may include a position sensor. In some embodiments, the position sensor may be at least one of the following types of sensors: a photoelectric sensor, a Hall sensor, and a laser sensor.

[0072] In some embodiments, the positioning monitoring component 133 may include a photoelectric sensor 1331 and a baffle 1332. The photoelectric sensor 1331 is relatively fixed to the housing of the mixing drive motor 131, and the baffle 1332 is relatively fixed to the tube holder 132. The photoelectric sensor 1331 is used to detect the position of the baffle 1332 and thus detect the rotation position of the tube holder 132. In some embodiments, the baffle 1332 is arranged corresponding to the notch 1321 of the tube holder 132 in the Z-axis direction (i.e., the third direction). In some embodiments, when the tube holder 132 drives the baffle 1332 to rotate, the rotation position of the tube holder 132 and thus the position of the notch 1321 can be determined according to the photoelectric signal change monitored by the photoelectric sensor 1331 and the corresponding monitoring program control, and then the corresponding control of the rotation or stop of the tube holder 132 is performed according to whether the position of the notch 1321 is facing the magnet 141. For example, when it is detected that the opening position of the notch 1321 is facing the magnet 141, the tube holder 132 is controlled to stop rotating so as to perform magnetic attraction processing. In some embodiments, the monitoring program includes a corresponding mapping relationship between the baffle 1332, the tube seat 132, the notch 1321 and / or the magnet 141, for example, a corresponding mapping relationship between the baffle 1332 and the notch 1321. In some embodiments, the photoelectric sensor 1331 may be an optical coupling sensor, and the baffle 1332 may be an optical coupling baffle. The use of an optical coupling component with a higher light sensing sensitivity can further improve the accuracy of notch positioning monitoring.

[0073] In some embodiments, the magnet 141 of the magnetic attraction mechanism 140 can cover multiple processing channels, so as to be used for magnetic attraction treatment of biological samples in multiple sample tubes 110 and / or biological samples in multiple reaction tubes 120 in multiple processing channels. The magnetic attraction treatment of multiple processing channels can be achieved only by a single magnet 141, and the device is easy to install and convenient to operate. In some embodiments, the magnetic attraction mechanism 140 may include multiple magnets 141, each magnet 141 is used to perform magnetic attraction treatment on biological samples in sample tubes 110 and / or biological samples in reaction tubes 120 in a processing channel, so as to facilitate the separate control of magnetic attraction treatment of different processing channels, meet the personalized requirements of different processing channels, and set a single magnet (such as a magnet) in a single processing channel, reducing the production cost of the magnet. In some embodiments, each sample tube 110 or reaction tube 120 and its corresponding magnet 141 are respectively provided with a corresponding positioning monitoring component 133, so as to achieve the positioning monitoring of each sample tube 110 or reaction tube 120 and the separate control of its magnetic attraction treatment.

[0074] In some embodiments, the magnetic attraction mechanism 140 may include a first magnetic attraction mechanism 142, and the first magnetic attraction mechanism 142 may include a first magnet 1421 and a first magnetic attraction drive motor 1422, and the first magnet 1421 is transmission-connected to the first magnetic attraction drive motor 1422; the first magnet 1421 is arranged between the sample tube 110 and the reaction tube 120; the first magnetic attraction drive motor 1422 can drive the first magnet 1421 to move between the sample tube 110 and the reaction tube 120 to perform magnetic attraction processing on the biological sample in the sample tube 110 and / or the biological sample in the reaction tube 120. For example, the first magnetic attraction drive motor 1422 can drive the first magnet 1421 to move between the sample tube 110 and the reaction tube 120, for example, it can move along the X-axis direction (i.e., the second direction) and / or the Y-axis direction (i.e., the first direction), so that the first magnet 1421 is close to the sample tube 110 and / or the reaction tube 120. In some embodiments, the first magnet 1421 may also be disposed on the other side of the sample tube 110 away from the reaction tube 120 along the X-axis direction.

[0075] In some embodiments, the first magnetic attraction mechanism 142 may further include a magnetic shielding cover 1423, which is disposed between the upper portion of the first magnet 1421 and the reaction tube 120, so as to adapt to the situation where the biological sample in the reaction tube 120 is small and close to the bottom, and achieve more effective corresponding magnetic attraction processing. In some embodiments, the magnetic shielding cover 1423 may be a magnetic shielding plate made of pure iron or iron-nickel alloy.

[0076] In some embodiments, the first magnet 1421 may include an upper sub-magnet 1421a and a lower sub-magnet 1421b arranged along the vertical direction (Z-axis direction (i.e., the third direction)); the magnetic shielding cover 1423 is disposed between the upper sub-magnet 1421a and the reaction tube 120, and also realizes the control of the size of the magnetic attraction area during the magnetic attraction treatment process, so as to more efficiently adapt to the magnetic attraction requirements of the reaction tube 120.

[0077] In some embodiments, the first magnetic attraction mechanism 142 may include a second magnetic attraction drive motor 1424, which is used to drive the first magnet 1421 to move in the vertical direction (Z-axis direction (i.e., the third direction)) to achieve independent control of the first magnetic attraction mechanism 142 in the vertical direction, further optimizing the movement control function of the first magnetic attraction mechanism 142 to meet more complex magnetic attraction processing work scenarios (for example, enabling the first magnet 1421 to perform magnetic attraction processing only on the lower part of the reaction tube).

[0078] In some embodiments, the first magnetic attraction mechanism 142 may include a first magnetic attraction bracket 1425 arranged along the Y-axis direction (i.e., the first direction). In some embodiments, the two ends of the first magnetic attraction bracket 1425 may be respectively arranged on two linear guide rails 1426 arranged along the X-axis direction (i.e., the second direction) to achieve better positioning guidance. In some embodiments, the first magnetic attraction drive motor 1422 is connected to the first magnetic attraction bracket 1425 to drive the first magnetic attraction mechanism 142 to move along the X-axis direction (i.e., the second direction). A lead screw 1427 is provided between the first magnetic attraction drive motor 1422 and the first magnetic attraction bracket 1425 along the X-axis direction (i.e., the second direction). In some embodiments, the first magnetic attraction drive motor 1422 may be a lead screw motor or any other possible type of motor. In some embodiments, the first magnetic attraction drive motor 1422 may drive the first magnetic attraction mechanism 142 to move along the X-axis direction (i.e., the second direction) through a lead screw nut mechanism.

[0079] In some embodiments, the magnetic attraction mechanism 140 may further include a second magnetic attraction mechanism 143. The second magnetic attraction mechanism 143 may include a second magnet 1431 and a third magnetic attraction drive motor 1432. The second magnet 1431 is transmission-connected to the third magnetic attraction drive motor 1432. The second magnet 1431 and the first magnet 1421 are arranged on opposite sides of the reaction tube 120. The third magnetic attraction drive motor 1432 can drive the second magnet 1431 to move relative to the reaction tube 120, for example, it can move along the X-axis direction (i.e., the second direction) and / or the Y-axis direction (i.e., the first direction) to perform magnetic attraction treatment on the biological sample in the reaction tube 120. Since the first magnetic attraction mechanism 142 and its first magnet 1421, the second magnetic attraction mechanism 143 and its second magnet 1431 are respectively arranged corresponding to the corresponding sample tube 110 and the reaction tube 120, it can further ensure the good magnetic attraction effect of the sample tube 110 and the reaction tube 120, and at the same time realize independent magnetic attraction processing control of the two, meet the higher requirements of magnetic attraction processing in the sample processing process, and improve the overall sample processing efficiency.

[0080] In some embodiments, the magnetic attraction area of ​​the second magnet 1431 may be different from the magnetic attraction area of ​​the first magnet 1421. In some embodiments, the magnetic attraction area of ​​the second magnet 1431 may be the same as the magnetic attraction area of ​​the first magnet 1421.

[0081] In some embodiments, the magnetic attraction area of ​​the second magnet 1431 is smaller than that of the first magnet 1421. When the second magnet 1431 is close to the reaction tube 120, the second magnet 1431 is close to the bottom of the reaction tube 120, and the magnetic attraction requirements of the reaction tube 120 are more specifically met through the separate magnetic attraction control of the second magnet 1431 of the second magnetic attraction mechanism 143.

[0082] In some embodiments, the second magnetic attraction mechanism 143 may also include a fourth magnetic attraction drive motor 1433; the fourth magnetic attraction drive motor 1433 is used to drive the second magnet 1431 to move in the vertical direction (Z-axis direction (ie, the third direction)) to achieve independent control of the second magnetic attraction mechanism 143 in the vertical direction.

[0083] In some embodiments, the second magnetic attraction mechanism 143 may include a second magnetic attraction bracket 1434 arranged along the Y-axis direction (i.e., the first direction). In some embodiments, the two ends of the second magnetic attraction bracket 1434 may be respectively arranged on two linear guide rails 1426 arranged along the X-axis direction (i.e., the second direction) to achieve better positioning guidance. In some embodiments, the third magnetic attraction drive motor 1432 is connected to the second magnetic attraction bracket 1434 to drive the second magnetic attraction mechanism 143 to move along the X-axis direction (i.e., the second direction). One or more guide shafts 1435 are provided between the third magnetic attraction drive motor 1432 and the second magnetic attraction bracket 1434 along the X-axis direction (i.e., the second direction). In some embodiments, the third magnetic attraction drive motor 1432 may be a screw motor or any other possible type of motor.

[0084] In some embodiments, when there are multiple processing channels, the first magnetic attraction mechanism 142 may include multiple first magnets 1421, and the second magnetic attraction mechanism 143 may include multiple second magnets 1431, and the multiple first magnets 1421 and the multiple second magnets 1431 correspond to the multiple sample tubes 110 and the reaction tubes 120, respectively. In some embodiments, the first magnetic attraction mechanism 142 may include a first magnetic attraction drive motor 1422 and a second magnetic attraction drive motor 1424; the second magnetic attraction mechanism 143 may include a third magnetic attraction drive motor 1432 and a fourth magnetic attraction drive motor 1433. In some embodiments, the first magnetic attraction mechanism 142 may include multiple first magnetic attraction drive motors 1422 and multiple second magnetic attraction drive motors 1424; the second magnetic attraction mechanism 143 may include multiple third magnetic attraction drive motors 1432 and multiple fourth magnetic attraction drive motors 1433. In some embodiments, the magnetic attraction processing of the second magnet 1431 and the first magnet 1421 between each processing channel can meet the predetermined magnetic attraction requirements (such as strong magnetic attraction, weak magnetic attraction, etc.) and do not interfere with each other. For the corresponding sample tube 110 and reaction tube 120 of each processing channel in the multiple processing channels, the corresponding first magnet 1421, the second magnet 1431 and the corresponding magnetic attraction drive motor are respectively set through the first magnetic attraction mechanism 142 and the second magnetic attraction mechanism 143, which can not only meet the needs of each independent magnetic attraction processing work, but also facilitate the drive movement control when each independent magnetic attraction processing is performed (especially suitable for complex sample processing scenarios where differentiated magnetic attraction processing and differentiated motor driver settings are required between different processing channels), thereby improving the overall processing efficiency of the sample processing device 100.

[0085] In some embodiments, the sample processing device 100 may further include a support structure 150 to provide good support for the sample tube 110, the reaction tube 120, the mixing mechanism 130 and the magnetic attraction mechanism 140, thereby improving the stability of the device and ensuring the flexibility of overall translation.

[0086] In some embodiments, the automatic extraction device may include one or more extraction channels. For example, one or more (e.g., 3, 6, 8, etc.) extraction channels may be set according to the number of samples to be processed at one time. Each extraction channel may include a sample tube, one or more (e.g., 1, 2, 3, etc.) reaction tubes, a reagent kit, a gun tip box, and a pipette. In some embodiments, each extraction channel can be covered by an external magnetic field for magnetically treating the biological samples in the sample tube and / or reaction tube of each channel. For example, the external magnetic field may be generated by a single magnet that can cover multiple extraction channels. For another example, each extraction channel may correspond to a magnet, and the external magnetic field may be generated by the corresponding magnet of each channel. By setting multiple extraction channels, each extraction channel may process biological samples, and the extraction processes of multiple extraction channels may be performed simultaneously, thereby improving the efficiency of biological sample processing.

[0087] Fig.18 Schematic diagram of multiple extraction channels of the automatic extraction device shown in some embodiments of this specification. Fig.18 As shown, the automatic extraction device 1800 may include multiple (e.g., 6) extraction channels 1810. A single extraction channel 1810 may include a sample tube 1811, a magnet 1812, a reaction tube 1813, a magnet 1814, a reagent box 1815, a pipette (not shown) and a gun tip box (not shown). Multiple extraction channels 1810 are arranged at intervals along the Y-axis direction (i.e., the first direction). The components of a single extraction channel 1810 extend along the X-axis direction (i.e., the second direction). Multiple sample tubes 1811, multiple reaction tubes 1813, multiple reagent boxes 1815, multiple pipettes and multiple gun tip boxes are aligned along the Y-axis direction (i.e., the first direction). In some embodiments, multiple magnets 1812 or multiple magnets 1814 can be replaced by a single magnet that can cover multiple extraction channels 1810 or multiple magnets superimposed. In some embodiments, multiple magnets 1814 can be omitted. In some embodiments, a single extraction channel 1810 may include a waste liquid box (not shown).

[0088] In some embodiments, the automatic extraction device described in this application specification (such as Figure 1-Figure 3 and / or Fig.18The automatic extraction device described in this specification can be used to extract and / or purify target biological substances. The following provides a method for using the automatic extraction device described in this specification to extract, separate and / or purify target biological substances. Before starting the automatic extraction device, the gun tip box is loaded onto the consumable support seat, the reagent kit is loaded onto the reagent kit support seat to load the entire device onto the consumable support seat, and the sample tube containing the biological sample and the reaction tube are loaded onto the tube seat. After loading is completed, the automatic extraction device can be started to automatically extract and / or purify the target biological substance.

[0089] In some embodiments, the method of using the automatic extraction device may include the following steps:

[0090] Step 1: Control the pipetting device to add the first reagent and the first magnetic beads of the consumable component into the sample tube of the sample processing device.

[0091] In some embodiments, the first magnetic bead is used to bind to impurities or to bind to a target biological substance. In some embodiments, the first magnetic bead and the first reagent can be a mixture placed in a cavity of the reagent kit.

[0092] Step 2: Control the mixing mechanism to drive the sample tube to mix.

[0093] By mixing, the biological sample and the reagent in the sample tube can be fully reacted and / or the first magnetic beads can be fully combined with the impurities or the target biological substance.

[0094] In some embodiments, the start and stop of the mixing process and the rotation speed and rotation direction of the sample tube are controlled by controlling the mixing drive motor.

[0095] Step 3: Control the magnetic suction mechanism to magnetically suction the mixed liquid in the sample tube.

[0096] In some embodiments, the first magnetic attraction drive motor can be controlled to control the first magnet to move closer to the sample tube, thereby obtaining a better magnetic attraction effect. In some embodiments, the second magnetic attraction drive motor can be controlled to control the first magnet to move up and down according to the amount of solution in the sample tube so as to better adsorb the first magnetic bead conjugate in the sample tube.

[0097] Step 4: After standing for a first preset time, control the pipetting device to collect the first supernatant in the sample tube, or remove the first supernatant in the sample tube to retain the adsorbed first magnetic beads.

[0098] In some embodiments, the first preset time may be the time when the automatic extraction device stops operating. In some embodiments, the first preset time may be manually set according to the extraction and purification process flow or may be a default setting in the automatic extraction device. In some embodiments, during the first preset time, the magnetic attraction mechanism maintains magnetic attraction to the mixed liquid in the sample tube.

[0099] The supernatant mentioned here refers to the transparent liquid above the sediment layer (e.g., the first magnetic bead conjugate) in the sample tube. Taking the first magnetic bead used to bind to impurities as an example, the first supernatant mainly includes the target biological substance to be extracted and a small amount of impurities. The method of using the automatic extraction device also includes the following steps:

[0100] Step 5: Control the pipetting device to add the first supernatant in the sample tube into the reaction tube, and control the pipetting device to add the second reagent and the second magnetic beads of the consumable assembly into the reaction tube.

[0101] In some embodiments, the second reagent is the same as the first reagent, and / or the first magnetic bead is the same as the second magnetic bead. For example, the second magnetic bead and the first magnetic bead have the same binding object. For example, the first magnetic bead and the second magnetic bead are both used to bind to impurities. For another example, the second magnetic bead may be the same as or different from the first magnetic bead. In some embodiments, the second reagent is different from the first reagent, and / or the second magnetic bead is different from the first magnetic bead. For example, the first magnetic bead is used to bind to impurities, and the second magnetic bead is used to bind to target biological substances. In some embodiments, the second reagent and the second magnetic bead can be a mixture placed in a cavity of the test kit.

[0102] Step 6: Control the mixing mechanism to drive the reaction tube to perform mixing.

[0103] In some embodiments, the start and stop of the mixing process and the rotation speed and rotation direction of the sample tube are controlled by controlling the mixing drive motor.

[0104] Step 7: Control the magnetic suction mechanism to magnetically suction the mixed liquid in the reaction tube.

[0105] In some embodiments, the third magnetic attraction drive motor can be controlled to move the second magnet closer to the reaction tube to obtain a better magnetic attraction effect. In some embodiments, the second magnetic attraction drive motor can be controlled to move the first magnet up and down to better adsorb the second magnetic bead conjugate in the sample tube.

[0106] Step 8: After standing for a second preset time, control the pipetting device to remove the second supernatant in the reaction tube to retain the adsorbed second magnetic beads, or collect the second supernatant in the reaction tube.

[0107] In some embodiments, the second preset time may be the time when the automatic extraction device stops operating. In some embodiments, the second preset time may be manually set according to the extraction and purification process flow or may be a default setting in the automatic extraction device. In some embodiments, during the second preset time, the magnetic attraction mechanism maintains magnetic attraction to the mixed liquid in the reaction tube.

[0108] In some embodiments, when the second magnetic beads are used to bind to impurities, the pipetting device is controlled to collect the second supernatant in the reaction tube, which is the target biological substance to be removed and / or purified.

[0109] In some embodiments, when the second magnetic beads are used to bind to the target biological substance, the pipetting device is controlled to remove the second supernatant in the reaction tube. The second supernatant in the reaction tube is waste liquid. In this case, step 9 can be continued. Step 9 includes the following steps:

[0110] Step 9a: After controlling the magnetic attraction mechanism to stop magnetic attraction, controlling the liquid transfer device to add the third reagent of the consumable component into the reaction tube, the third reagent can elute the target biological substance from the second magnetic beads.

[0111] Step 9b: Control the mixing mechanism to drive the reaction tube to perform mixing.

[0112] Step 9c: Control the magnetic attraction mechanism to magnetically attract the mixed liquid in the reaction tube.

[0113] Step 9d: After standing for a third preset time, control the pipetting device to collect the third supernatant in the reaction tube.

[0114] The third supernatant is the target biological substance to be removed and / or purified. In some embodiments, the third preset time may be the time when the automatic extraction device stops operating. In some embodiments, the third preset time may be manually set according to the extraction and purification process flow or may be a default setting in the automatic extraction device. In some embodiments, during the third preset time, the magnetic attraction mechanism maintains magnetic attraction to the mixed liquid in the reaction tube.

[0115] In some embodiments, before step 9a, steps 5-8 may be repeated one or more times (eg, twice) to sufficiently remove impurities through the waste liquid.

[0116] In some embodiments, step 1 and step 5 include aspiration and discharge operations. When performing aspiration, the aspiration step includes: after assembling the gun tip, controlling the moving beam drive motor to move the moving beam along the X-axis direction (i.e., the second direction) to the position where the aspiration operation needs to be performed, so that the pipette is located above the position; controlling the pipetting device drive motor to move the pipette downward along the Z-axis direction (i.e., the third direction) until the gun tip contacts the solution; controlling the piston drive motor to drive the piston to move upward to achieve the aspiration operation. In some embodiments, assembling the gun tip may include: controlling the moving beam drive motor to move the moving beam to the gun tip box, so that the pipette is located above the gun tip to be assembled; controlling the pipetting device drive motor to move the pipette downward along the Z-axis direction (i.e., the third direction) until the pipette contacts the gun tip and is sealed; moving the pipette upward along the Z-axis direction (i.e., the third direction) to remove the gun tip from the gun tip box. In some embodiments, assembling the gun tip may include: after the pipette is located above the gun tip, controlling the pipetting device drive motor to move the pipette downward along the third direction to a suitable position; controlling the piston drive motor to make the drive plate push the push rod and the push plate to move downward until the suction tip and the gun tip are in contact and sealed connection, and the spring provided on the push rod can serve as a buffer for the piston drive motor, slowing down the impact transmitted to the suction tip by the piston drive motor, avoiding a violent collision between the suction tip and the gun tip, thereby avoiding damage to the suction tip and / or the gun tip. In some embodiments, the position of the gun tip box relative to the base 201 can be adjusted along the Y-axis direction (i.e., the first direction) by controlling the consumable assembly drive motor so that the gun tip in the gun tip box is aligned with the pipette.

[0117] In some embodiments, when the aspiration operation is performed, a puncture step is required before the aspiration step. The puncture step may include: controlling the puncture mechanism to move along the third direction so that the puncture mechanism punctures the sealing layer of the reagent box. In some embodiments, the puncture step may also include: controlling the moving beam drive motor to move the moving beam to the reagent box so that the pipette is located above the reagent box; controlling the pipetting device drive motor to move the pipette downward along the Z-axis direction (i.e., the third direction) to a suitable position; controlling the piston drive motor to drive the drive plate to push the push rod and the push plate downward to the puncture mechanism to puncture the sealing layer, so that the gun tip can pass through the punctured hole in the sealing layer to absorb the reagent. In some embodiments, the position of the reagent box relative to the base 201 can be adjusted along the Y-axis direction (i.e., the first direction) by controlling the consumable component drive motor to align the reagent box cavity with the pipette.

[0118] In some embodiments, when performing a liquid discharge operation, the liquid discharge step includes: controlling the moving beam drive motor to move the moving beam to a position where the liquid discharge operation is required, so that the pipette is located above the position; controlling the liquid transfer device drive motor to move the pipette downward along the Z-axis direction (i.e., the third direction) until the gun tip contacts the solution; and controlling the piston drive motor to drive the piston to move downward to achieve the liquid discharge operation. The detailed steps of the liquid discharge operation are similar to those of the liquid suction operation and will not be repeated here.

[0119] Taking the first magnetic beads used to bind to the target biological substance as an example, the first supernatant is waste liquid. The method of using the automatic extraction device also includes: controlling the pipetting device to remove the first supernatant in the sample tube to retain the adsorbed first magnetic beads, and then controlling the magnetic attraction mechanism to stop magnetic attraction; controlling the pipetting device to add the fourth reagent of the consumable component into the sample tube for eluting the target biological substance from the first magnetic beads; controlling the mixing mechanism to drive the sample tube to mix; controlling the magnetic attraction mechanism to magnetically attract the mixed liquid in the sample tube; and controlling the pipetting device to collect the fourth supernatant in the sample tube, which is the target biological substance to be extracted and / or purified.

[0120] The beneficial effects that may be brought about by the embodiments of this specification include but are not limited to: (1) by arranging the pipetting device, consumables assembly and sample processing device on the gantry, the operator only needs to load the consumables and biological samples into the automatic extraction device to automatically operate the automatic extraction device, thereby realizing the mechanical automation of extracting the target biological substance and saving a lot of labor costs; (2) by arranging multiple extraction channels in parallel on the automatic extraction device, multiple biological samples can be processed simultaneously through multiple extraction channels, thereby improving the efficiency of biological sample processing and improving the quality and yield of target biological substance extraction; (3) by automatically and mechanizedly controlling the mixing mechanism and magnetic suction mechanism in the sample processing device, the mechanized operation of mixing and magnetic suction processing in the biological sample processing process is realized, thereby improving the operation efficiency; (4) by controlling the movement of the pipetting device in multiple directions through the pipetting device driving motor and the piston driving motor, the mechanized pipetting operation in the biological sample processing process is realized, and it can meet the biological sample The requirements for pipetting operations at different stages of the processing process are met, thereby improving the mechanization of the automatic extraction device; (5) By setting a puncture mechanism in the pipetting device, the sealing layer of the reagent box can be mechanically punctured before aspirating the liquid from the reagent box, thereby improving the automation of the pipetting operation and the biological sample processing process; (6) By controlling the movement of the consumable assembly by the consumable drive motor, it can be met that when there are many cavities and / or gun tips in the reagent box, the automatic extraction device can be arranged in multiple columns so that the channel direction is not too long, so that the structure of the automatic extraction device can be reasonably set; (7) By loading multiple reagent boxes on the reagent box support, it is convenient for the operator to load and remove multiple reagent boxes onto the automatic extraction device as a whole, thereby improving the operational convenience of the automatic extraction device; (8) By controlling the movement of the mixing mechanism and the magnetic attraction mechanism in multiple directions by the mixing drive motor and the magnetic attraction drive motor, the automated operation efficiency of the sample processing device and the automatic extraction device is further improved, and a variety of mixing processing requirements and magnetic attraction operation requirements can be met. It should be noted that different embodiments may produce different beneficial effects. In different embodiments, the beneficial effects that may be produced may be any one or a combination of the above, or any other beneficial effects that may be obtained.

[0121] The basic concepts have been described above. Obviously, for those skilled in the art, the above detailed disclosure is only for example and does not constitute a limitation of this specification. Although not explicitly stated here, those skilled in the art may make various modifications, improvements and corrections to this specification. Such modifications, improvements and corrections are suggested in this specification, so such modifications, improvements and corrections still belong to the spirit and scope of the exemplary embodiments of this specification.

[0122] At the same time, this specification uses specific words to describe the embodiments of this specification. For example, "one embodiment", "an embodiment", and / or "some embodiments" refer to a certain feature, structure or characteristic related to at least one embodiment of this specification. Therefore, it should be emphasized and noted that "one embodiment" or "an embodiment" or "an alternative embodiment" mentioned twice or more in different positions in this specification does not necessarily refer to the same embodiment. In addition, certain features, structures or characteristics in one or more embodiments of this specification can be appropriately combined.

[0123] In addition, unless explicitly stated in the claims, the order of processing elements and sequences, the use of alphanumeric characters, or the use of other names in this specification are not intended to limit the order of the processes and methods of this specification. Although the above disclosure discusses some embodiments currently considered useful through various examples, it should be understood that such details are only for illustrative purposes, and the attached claims are not limited to the disclosed embodiments. On the contrary, the claims are intended to cover all modifications and equivalent combinations that are consistent with the essence and scope of the embodiments of this specification. For example, although the system components described above can be implemented by hardware devices, they can also be implemented only by software solutions, such as installing the described system on an existing server or mobile device.

[0124] Similarly, it should be noted that in order to simplify the description disclosed in this specification and thus help understand one or more embodiments, in the above description of the embodiments of this specification, multiple features are sometimes combined into one embodiment, figure or description thereof. However, this disclosure method does not mean that the features required by the subject matter of this specification are more than the features mentioned in the claims. In fact, the features of the embodiments are less than all the features of the single embodiment disclosed above.

[0125] In some embodiments, numbers describing the number of components and attributes are used. It should be understood that such numbers used in the description of the embodiments are modified by the modifiers "about", "approximately" or "substantially" in some examples. Unless otherwise specified, "about", "approximately" or "substantially" indicate that the numbers are allowed to vary by ±20%. Accordingly, in some embodiments, the numerical parameters used in the specification and claims are approximate values, which may change according to the required features of individual embodiments. In some embodiments, the numerical parameters should take into account the specified significant digits and adopt the general method of retaining digits. Although the numerical domains and parameters used to confirm the breadth of their range in some embodiments of this specification are approximate values, in specific embodiments, the setting of such numerical values ​​is as accurate as possible within the feasible range.

[0126] Each patent, patent application, patent application publication, and other materials, such as articles, books, specifications, publications, documents, etc., cited in this specification is hereby incorporated by reference in its entirety. Except for application history documents that are inconsistent with or conflicting with the content of this specification, documents that limit the broadest scope of the claims of this specification (currently or later attached to this specification) are also excluded. It should be noted that if the descriptions, definitions, and / or use of terms in the materials attached to this specification are inconsistent or conflicting with the content of this specification, the descriptions, definitions, and / or use of terms in this specification shall prevail.

[0127] Finally, it should be understood that the embodiments in this specification are only used to illustrate the principles of the embodiments of this specification. Other variations may also fall within the scope of this specification. Therefore, as an example and not a limitation, alternative configurations of the embodiments of this specification may be considered consistent with the teachings of this specification. Accordingly, the embodiments of this specification are not limited to the embodiments explicitly introduced and described in this specification.

Claims

1. A gantry, comprising a base and a shift beam, characterized in that: The shifting beam is movable relative to the base; The shifting beam comprises one or more columns arranged perpendicularly to the base, and a crossbeam perpendicularly to the one or more columns, wherein the crossbeam is connected to the one or more columns.

2. The gantry as described in claim 1, wherein the shifting beam comprises two columns arranged perpendicular to the base, and a cross beam perpendicular to the two columns, and the cross beam is fixedly connected to the two columns.

3. The gantry as claimed in claim 1 or 2, characterized in that: The gantry also includes a beam-shifting guide rail, which is installed on the base. The beam-shifting guide rail is connected to the base via the beam-shifting guide rail, and the one or more columns can move on the beam-shifting guide rail.

4. The gantry as described in claim 3, further comprising a beam shifting drive motor and a beam shifting screw nut pair, the beam shifting is connected to the beam shifting screw nut pair, the beam shifting drive motor can drive the beam shifting screw nut pair to rotate, thereby driving the beam shifting to move on the beam shifting guide rail.

5. An automatic extraction device for extracting target biological substances, characterized in that: The automatic extraction device comprises: a sample processing device, a consumable component, a pipetting device and a gantry as described in any one of claims 1 to 4; The sample processing device includes a mixing mechanism and a magnetic attraction mechanism, which are used to perform mixing and magnetic attraction processing on the biological sample respectively; The sample processing device and the consumable component are arranged on the base of the gantry, and the pipetting device is arranged on the shifting beam of the gantry. The pipetting device can be used to implement a pipetting operation between the consumable component and the sample processing device.

6. The automatic extraction device according to claim 5, characterized in that: The sample processing device includes at least one of a sample tube and a reaction tube; The magnetic attraction mechanism includes a magnet, and the magnet can move relative to the sample tube and / or the reaction tube to perform magnetic attraction processing on the biological sample in the sample tube and / or the biological sample in the reaction tube.

7. The automatic extraction device according to claim 5, characterized in that: The mixing mechanism comprises a mixing drive motor and a tube holder, and the mixing drive motor is drivingly connected to the tube holder; The sample tube and / or the reaction tube are arranged in the tube holder; The mixing drive motor can drive the tube holder to move, thereby driving the biological sample in the sample tube and / or the biological sample in the reaction tube to be mixed.

8. The automatic extraction device according to claim 5, characterized in that: The consumables assembly includes a reagent kit and a gun tip box, the reagent kit includes one or more cavities for containing reagents, and the gun tip box includes one or more gun tips; The pipetting device comprises a pipette, and the pipette can be detachably and hermetically connected to the gun head. When the pipette is hermetically connected to the gun head, the pipette can control the gun head to achieve liquid aspiration and liquid discharge.

9. The automatic extraction device according to claim 8, characterized in that: The pipette comprises a cavity and a piston, wherein a first end of the piston is capable of sealing the cavity and the piston is capable of moving within the cavity.

10. The automatic extraction device according to claim 5, characterized in that: The liquid transfer device can move relative to the transfer beam to achieve liquid suction and liquid discharge.

11. The automatic extraction device according to claim 5, characterized in that: The pipetting device also includes a piston drive assembly and a support plate, the piston drive assembly includes a piston drive motor, a drive plate and a piston guide rail, the drive plate is connected to the second end of the piston, and the drive plate is transmission-connected to the piston drive motor, the piston guide rail is arranged on the support plate, the drive plate is connected to the piston guide rail, and the drive plate can move toward or away from the base along the piston guide rail.

12. The automatic extraction device according to claim 8, characterized in that: The reagent kit comprises a sealing layer, and the pipetting device further comprises a puncturing mechanism, and the puncturing mechanism can puncture the sealing layer.

13. The automatic extraction device according to claim 9, characterized in that: The cavity has a suction tip, and the pipetting device also includes a push plate and a push rod, wherein the push plate is connected to the push rod, and a spring is sleeved on the push rod. The push rod can push the push plate to move toward the base, and the spring is compressed during the process in which the push rod pushes the push plate to move toward the base. The suction tip and the puncture mechanism are arranged on a side of the push plate facing the base.

14. The automatic extraction device according to claim 11, characterized in that: The pipetting device also includes a pipetting device drive assembly, which includes a pipetting device drive motor and a pipetting device guide rail. The support plate is connected to the shift beam via the pipetting device guide rail. The pipetting device drive motor is transmission-connected to the support plate to drive the support plate to move toward or away from the base along the pipetting device guide rail relative to the shift beam.

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