CAR-T cell amplifier

Abstract

The invention relates to the technical field of amplifiers, and discloses a CAR-T cell amplifier which comprises a shell, a cell refrigerating box and a control room, a partition plate is fixedly connected to the inner wall of one side of the shell, a plurality of through holes are formed in the partition plate, an extrusion assembly for blocking the through holes is arranged at the top of the shell, a plurality of connecting holes are formed in the bottom of the shell, and the connecting holes are communicated with the partition plate. A plurality of connecting holes are formed in the shell, sliding barrels are movably connected into the multiple connecting holes, top plates are fixedly connected to the two sides of the tops of the sliding barrels, culture dishes are arranged on the tops of the sliding barrels, multiple sliding rods are movably inserted into the bottom of the shell, and first fixing plates are fixedly connected between the bottoms of the multiple adjacent sliding rods. According to the device, a worker can conveniently and independently take out the culture dishes, the situation that when the single culture dish is taken out, the remaining culture dishes are completely exposed, and pollution is likely to be caused is avoided, and the height of the moved first fixing plate can be fixed through cooperative use of the first magnetic plate and the second magnetic plate.

Description

Technical Field

[0001] The present invention relates to the technical field of amplifiers, and in particular to a CAR-T cell amplifier. Background Art

[0002] T cells, also called T lymphocytes, are a type of white blood cell in the human body. They are derived from peripheral blood and play an immune role in the human body. Their role is equivalent to that of a "warrior" in the human body, able to resist and eliminate "enemies" such as infection, tumors, and foreign bodies. In the laboratory, technicians use genetic engineering technology to activate T cells and install a positioning and navigation device CAR, transforming the ordinary "warrior" T cell into a "super soldier", namely CAR-T cell.

[0003] After searching, a Chinese patent with announcement number CN111197006B discloses a CAR-T cell amplifier, including: a main body, a culture bag, a cell refrigerator for refrigerating culture reagents, and a pipeline delivery system capable of delivering the liquid in the cell refrigerator to the culture bag. If the culture reagent is needed, the culture reagent can be heated by a pipeline heating module. However, the following defects still exist. When it is necessary to take out the culture dish, the door panel needs to be opened to take it out. After the door panel is opened, various bacteria, fungi and other microorganisms in the external environment enter the amplifier, which may contaminate the remaining CAR-T cells in the culture dish, causing cell growth stagnation or even death. Summary of the invention

[0004] 1. Technical issues to be solved

[0005] In view of the shortcomings of the prior art, the present invention provides a CAR-T cell amplifier, which is mainly used to solve the problem that when the door panel is opened, various microorganisms such as bacteria and fungi in the external environment enter the amplifier and may contaminate the CAR-T cells in the culture dish, causing cell growth stagnation or even death.

[0006] (II) Technical solution

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A CAR-T cell amplifier comprises a shell, a cell refrigerator and a control room, wherein a partition is fixedly connected to an inner wall of one side of the shell, a plurality of through holes are provided inside the partition, an extrusion assembly for blocking the through holes is provided on the top of the shell, a plurality of connection holes are provided at the bottom of the shell, a slide cylinder is movably connected to the inside of the plurality of connection holes, a top plate is fixedly connected to both sides of the top of the slide cylinder, a culture dish is provided on the top of the slide cylinder, a plurality of slide rods are movably inserted at the bottom of the shell, a first fixed plate is fixedly connected between the bottoms of the plurality of adjacent slide rods, a second telescopic rod is fixedly connected to the upper surface of the first fixed plate, and an extended end of the second telescopic rod is fixed to the slide cylinder, and a first telescopic rod is sleeved on the outer side of the second telescopic rod. Three springs, and both ends of the third spring are respectively fixed to the slide cylinder and the first fixed plate, a plurality of positioning components are installed on the bottom inner wall of the shell, the positioning component includes two sliders, the two sliders are arranged on the bottom inner wall of the shell, the top of the slider is fixedly connected to a limiting block, and the limiting block contacts the top of the culture dish, both ends of the slider are fixedly connected to an extension rod, one side of the slider is fixedly connected to two guide rods, the bottom inner wall of the shell is fixedly connected to a second fixed plate, and the guide rod is slidably connected to the second fixed plate, one side of the slider is fixedly connected to a second spring, and the second spring is fixed to the second fixed plate, the top of the slide rod passes through the shell and is provided with an inclined surface, and the inclined surface contacts the extension rod.

[0009] Furthermore, the extrusion assembly includes a plurality of first telescopic rods, which are fixed to the top inner wall of the shell by bolts, the extended ends of the plurality of first telescopic rods are fixedly connected to a pressure plate, and the pressure plate blocks the through hole, the top plate can contact the bottom of the pressure plate, the bottom of the pressure plate is fixedly connected to a sealing gasket, the outer side of the first telescopic rod is sleeved with a first spring, and the two ends of the first spring are respectively fixed to the pressure plate and the top inner wall of the shell.

[0010] On the basis of the above-mentioned solution, the bottom outer wall of the shell is fixedly connected to the limiting frame, and the bottom of the first fixed plate is in contact with the limiting frame.

[0011] As a further solution of the present invention, a first magnetic plate is fixedly connected to the upper surface of the first fixing plate, a second magnetic plate is fixedly connected to the bottom outer wall of the shell, and the first magnetic plate can be in contact with the second magnetic plate.

[0012] Furthermore, a groove is provided on the top of the slide cylinder, and the culture dish is stuck in the groove.

[0013] On the basis of the above scheme, a mounting opening is provided on one side of the shell, a door panel is movably connected in the mounting opening, and the door panel and the shell are fixed by a lock. An observation port is provided on one side of the shell, and a transparent plate is clamped in the observation port.

[0014] As a further solution of the present invention, a support frame is fixedly connected between the cell refrigerator and the bottom outer wall of the control room.

[0015] Furthermore, the cell refrigerator and the control room are respectively fixed on two sides of the shell, two sterilization lamps are fixedly connected to the inner walls on both sides of the shell, and a humidifier is fixedly connected to the bottom inner wall of the shell.

[0016] (III) Beneficial effects

[0017] Compared with the prior art, the present invention provides a CAR-T cell amplifier, which has the following beneficial effects:

[0018] 1. The present invention uses a partition to separate the upper and lower closed spaces of the shell, which makes it convenient for the staff to move the culture dishes they need to use to the top of the partition separately, and convenient for the staff to take out the culture dishes separately, avoiding the exposure of the remaining culture dishes when taking a single culture dish, which is easy to cause contamination, and improves the taking effect of the device.

[0019] 2. The present invention uses the first magnetic plate and the second magnetic plate in combination to fix the height of the first fixed plate after movement, thereby fixing the height of the culture dish moved above the partition, thereby improving the stability of the device.

[0020] 3. The present invention can not only position the culture dish through the groove, making it convenient for the staff to install and place it, but also limit the position of the culture dish through the limit block, so that the culture dish can be stably placed in the shell, thereby improving the stability of the culture dish.

[0021] 4. The present invention supports the bottom of the first fixing plate by means of a limiting frame, thereby limiting the height of the first fixing plate, preventing the slide cylinder from being separated from the connecting hole, and improving the use effect of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic diagram of the three-dimensional structure of a CAR-T cell amplifier proposed in the present invention;

[0023] Figure 2 This is a partial cross-sectional structural schematic diagram of a CAR-T cell amplifier proposed in the present invention;

[0024] Figure 3 This is a schematic diagram of the enlarged structure of part A of a CAR-T cell amplifier proposed in the present invention;

[0025] Figure 4 This is a schematic cross-sectional structure diagram of a first fixing plate of a CAR-T cell amplifier proposed in the present invention;

[0026] Figure 5This is a schematic diagram of the sliding tube amplification structure of a CAR-T cell amplifier proposed in the present invention.

[0027] In the figure: 1. shell; 2. door panel; 3. cell refrigerator; 4. support frame; 5. transparent plate; 6. first telescopic rod; 7. first spring; 8. germicidal lamp; 9. pressure plate; 10. sealing gasket; 11. partition; 12. slide cylinder; 13. slide rod; 14. first fixed plate; 15. humidifier; 16. culture dish; 17. limit block; 18. slider; 19. second fixed plate; 20. second spring; 21. guide rod; 22. extension rod; 23. inclined plane; 24. top plate; 25. first magnetic plate; 26. second telescopic rod; 27. third spring; 28. limit frame; 29. ​​second magnetic plate; 30. through hole; 31. groove. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Reference Figure 1-Figure 5A CAR-T cell amplifier comprises a shell 1, a cell refrigerator 3 and a control room. A partition 11 is fixed to an inner wall of one side of the shell 1 by bolts. A plurality of through holes 30 are provided inside the partition 11. An extrusion assembly for blocking the through holes 30 is provided on the top of the shell 1. A plurality of connecting holes are provided at the bottom of the shell 1. A slide cylinder 12 is slidably connected to the interior of the plurality of connecting holes. Top plates 24 are fixed to the top sides of the slide cylinder 12 by bolts. The top of the slide cylinder 12 passes through the shell 1 and is provided with a culture dish 16. A plurality of slide rods 13 are movably inserted at the bottom of the shell 1. A first fixing plate 14 is fixed between the bottoms of the plurality of adjacent slide rods 13 by bolts. The top of the first fixing plate 14 A second telescopic rod 26 is fixed to the surface by bolts, and the extended end of the second telescopic rod 26 is fixed to the slide tube 12, and a third spring 27 is sleeved on the outer side of the second telescopic rod 26, and the two ends of the third spring 27 are respectively fixed to the slide tube 12 and the first fixed plate 14, and a plurality of positioning components are installed on the bottom inner wall of the shell 1, and the positioning components include two sliders 18, and the two sliders 18 are arranged on the bottom inner wall of the shell 1, and the top of the slider 18 is fixed with a limited block 17 by bolts, and the limited block 17 is in contact with the top of the culture dish 16, and the two ends of the slider 18 are fixed with an extension rod 22 by bolts, select the first fixed plate 14 corresponding to the culture dish 16 to be taken out, and push The first fixing plate 14 is moved to make the slide bar 13 move upward along the shell 1, so that the inclined surface 23 on the slide bar 13 squeezes the extension rod 22 to move outward respectively, thereby making the slide block 18 drive the limit block 17 to move outward respectively, and the second spring 20 contracts until the limit block 17 is separated from the culture dish 16. One side of the slide bar 18 is fixed with two guide rods 21 by bolts, and the bottom inner wall of the shell 1 is fixed with a second fixing plate 19 by bolts, and the guide rod 21 is slidably connected with the second fixing plate 19, and the guide rod 21 can guide the slide bar 18 so that it can move stably, and one side of the slide bar 18 is welded with a second spring 20, and the second spring 20 is connected with the second The fixed plates 19 are fixed, the top end of the slide rod 13 passes through the shell 1 and is provided with a slope 23, and the slope 23 contacts the extension rod 22, and the first fixed plate 14 moves upward while the slide cylinder 12 moves upward along the connecting hole, so that the culture dish 16 and the top plate 24 move upward, and the top plate 24 separates the extrusion assembly from the through hole 30 until the culture dish 16 passes through the through hole 30, and the slide cylinder 12 blocks the through hole 30, and the culture dish 16 is located above the partition 11, and the first magnetic plate 25 on the first fixed plate 14 contacts the second magnetic plate 29, so as to fix the height of the first fixed plate 14, and then the staff opens the door panel 2 and takes out the culture dish 16 on the top of the partition 11.

[0030] In particular, the extrusion assembly includes a plurality of first telescopic rods 6, which are fixed to the top inner wall of the shell 1 by bolts, and the extended ends of the plurality of first telescopic rods 6 are fixed with a pressure plate 9 by bolts, and the pressure plate 9 blocks the through hole 30, and the top plate 24 can contact the bottom of the pressure plate 9, and a sealing gasket 10 is bonded to the bottom of the pressure plate 9, and the sealing gasket 10 can make the contact between the pressure plate 9 and the through hole 30 closer, and the outer side of the first telescopic rod 6 is sleeved with a first spring 7, and the two ends of the first spring 7 are respectively fixed to the pressure plate 9 and the top inner wall of the shell 1, and the top plate 24 moves upward to squeeze the pressure plate 9, so that the first telescopic rod 6 contracts, and the first spring 7 contracts, thereby separating the pressure plate 9 from the through hole 30.

[0031] It should be noted that the bottom outer wall of the shell 1 is fixed with a limit frame 28 by bolts, and the bottom of the first fixed plate 14 is in contact with the limit frame 28, and the limit frame 28 can limit the height of the first fixed plate 14. The upper surface of the first fixed plate 14 is fixed with a first magnetic plate 25 by bolts, and the bottom outer wall of the shell 1 is fixed with a second magnetic plate 29 by bolts, and the first magnetic plate 25 can be in contact with the second magnetic plate 29. By using the first magnetic plate 25 and the second magnetic plate 29 in coordination, the height of the first fixed plate 14 after moving is fixed, and then the height of the culture dish 16 moved above the partition 11 is fixed. A groove 31 is opened on the top of the slide 12, and the culture dish 16 is stuck in the groove 31. The groove 31 is fixed to the bottom outer wall of the shell 11. 1 can position the culture dish 16, which is convenient for the staff to place it stably. A mounting port is opened on one side of the shell 1, and a door panel 2 is rotatably connected in the mounting port, and the door panel 2 and the shell 1 are fixed by a lock. An observation port is opened on one side of the shell 1, and a transparent plate 5 is clamped in the observation port, which is convenient for the staff to observe the culture dish 16 inside the shell 1. A support frame 4 is fixed between the cell refrigerator 3 and the bottom outer wall of the control room by bolts. The cell refrigerator 3 and the control room are respectively fixed on both sides of the shell 1. Two sterilization lamps 8 are fixed to the inner walls of both sides of the shell 1 by bolts, so that the upper half of the space of the shell 1 separated by the partition 11 is sterilized, and a humidifier 15 is fixed to the bottom inner wall of the shell 1 by bolts.

[0032] Working principle: when it is necessary to take out the culture dish 16 containing CAR-T cells, select the first fixed plate 14 corresponding to the culture dish 16 to be taken out, push the first fixed plate 14, and move the slide bar 13 upward along the shell 1, so that the inclined surface 23 on the slide bar 13 squeezes the extension rod 22 to move outward respectively, and then the slider 18 drives the limit block 17 to move outward respectively, and the second spring 20 contracts until the limit block 17 is separated from the culture dish 16, and at the same time, the slide cylinder 12 moves upward along the connecting hole, so that the culture dish 16 and the top plate 24 move upward, at this time, the top plate 24 squeezes the pressing plate 9, so that the first telescopic rod 6 contracts, and the first spring 7 contracts until the culture dish 16 passes through the through hole 30, at this time, the slide cylinder 12 blocks the through hole 30, and the culture dish 16 is located above the partition 11, and at the same time, the first magnetic plate 25 on the first fixed plate 14 contacts the second magnetic plate 29 to achieve the height fixation of the first fixed plate 14, and then the staff opens the door panel 2 to take out the culture dish 16 on the top of the partition 11.

[0033] The electrical components mentioned in this article are all connected to an external main controller and 220V mains electricity, and the main controller can be a conventional known device for controlling a computer or the like.

[0034] In the description of this article, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection; it can be a mechanical connection, it can be an electrical connection, or it can be a direct connection. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0035] In the description herein, it should be noted that the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.

[0036] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A CAR-T cell expander, comprising a housing (1), a cell refrigerator (3) and a control chamber, characterized in that: A partition (11) is fixedly connected to the inner wall of one side of the shell (1), and a plurality of through holes (30) are provided inside the partition (11). An extrusion assembly for blocking the through holes (30) is provided at the top of the shell (1). A plurality of connection holes are provided at the bottom of the shell (1), and a slide cylinder (12) is movably connected to the inside of the plurality of connection holes. Both sides of the top of the slide cylinder (12) are fixedly connected to a top plate (24). A culture dish (16) is provided on the top of the slide cylinder (12). A plurality of slide rods (13) are movably inserted at the bottom of the shell (1), and a first fixed plate (14) is fixedly connected between the bottoms of adjacent plurality of slide rods (13). A second telescopic rod (26) is fixedly connected to the upper surface of the first fixed plate (14), and an extended end of the second telescopic rod (26) is fixed to the slide cylinder (12). A third spring (27) is sleeved on the outer side of the second telescopic rod (26), and two ends of the third spring (27) are respectively connected to the slide cylinder (12) and The first fixing plate (14) is fixed to the first fixing plate (14), and a plurality of positioning components are installed on the bottom inner wall of the shell (1). The positioning components include two sliders (18), which are arranged on the bottom inner wall of the shell (1). The top of the slider (18) is fixedly connected to a limit block (17), and the limit block (17) contacts the top of the culture dish (16). Both ends of the slider (18) are fixedly connected to an extension rod (22). One side of the slider (18) is fixedly connected to two guide rods (21). The bottom inner wall of the shell (1) is fixedly connected to a second fixing plate (19), and the guide rod (21) is slidably connected to the second fixing plate (19). One side of the slider (18) is fixedly connected to a second spring (20), and the second spring (20) is fixed to the second fixing plate (19). The top of the slider (13) passes through the shell (1) and is provided with an inclined surface (23), and the inclined surface (23) contacts the extension rod (22).

2. A CAR-T cell amplifier according to claim 1, characterized in that: The extrusion assembly comprises a plurality of first telescopic rods (6), the plurality of first telescopic rods (6) being fixed to the top inner wall of the shell (1) by bolts, the extended ends of the plurality of first telescopic rods (6) being fixedly connected to a pressure plate (9), and the pressure plate (9) blocking the through hole (30), the top plate (24) being able to contact the bottom of the pressure plate (9), the bottom of the pressure plate (9) being fixedly connected to a sealing gasket (10), the outer side of the first telescopic rod (6) being sleeved with a first spring (7), and the two ends of the first spring (7) being respectively fixed to the pressure plate (9) and the top inner wall of the shell (1).

3. A CAR-T cell amplifier according to claim 1, characterized in that: The bottom outer wall of the housing (1) is fixedly connected to the limiting frame (28), and the bottom of the first fixed plate (14) is in contact with the limiting frame (28).

4. A CAR-T cell amplifier according to claim 1, characterized in that: A first magnetic plate (25) is fixedly connected to the upper surface of the first fixing plate (14), a second magnetic plate (29) is fixedly connected to the bottom outer wall of the shell (1), and the first magnetic plate (25) can be in contact with the second magnetic plate (29).

5. A CAR-T cell amplifier according to claim 1, characterized in that: The top of the slide cylinder (12) is provided with a groove (31), and the culture dish (16) is stuck in the groove (31).

6. A CAR-T cell amplifier according to claim 1, characterized in that: A mounting opening is provided on one side of the shell (1), a door panel (2) is movably connected in the mounting opening, and the door panel (2) and the shell (1) are fixed by a lock; an observation opening is provided on one side of the shell (1), and a transparent plate (5) is snap-fitted in the observation opening.

7. A CAR-T cell amplifier according to claim 6, characterized in that: A support frame (4) is fixedly connected between the cell cold storage box (3) and the bottom outer wall of the control room.

8. A CAR-T cell amplifier according to claim 1, characterized in that: The cell refrigerator (3) and the control room are respectively fixed on both sides of the shell (1), two sterilization lamps (8) are fixedly connected to the inner walls on both sides of the shell (1), and a humidifier (15) is fixedly connected to the bottom inner wall of the shell (1).

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