Composition for inhibiting color change of ascorbyl glucoside and application of composition in antioxidant skin care product

Abstract

The invention discloses a composition for inhibiting color change of ascorbyl glucoside and application of the composition in antioxidant skin care products, and particularly belongs to the field of dressing preparations. The composition for inhibiting ascorbyl glucoside discoloration is prepared from the following components in parts by mass: 10 to 15 parts of ascorbyl glucoside, 2 to 4 parts of tricholoma matsutake extract, 2 to 5 parts of cymbidium hookerianum root extract and 1 to 3 parts of astaxanthin. According to the composition provided by the invention, the stability is good when the ascorbyl glucoside, the tricholoma matsutake extract, the cymbidium hookerianum root extract and the astaxanthin are compounded for use, so that the color change of the ascorbyl glucoside over time is effectively inhibited, and the oxidation resistance of the ascorbyl glucoside is enhanced; the invention has important significance for effective application of ascorbyl glucoside in skin care products.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and in particular relates to a composition for inhibiting discoloration of ascorbyl glucoside and application of the composition in antioxidant skin care products. Background Art

[0002] In cosmetics, ascorbyl glucoside is a whitening antioxidant component. Its main mechanism of action is to inhibit the activity of tyrosinase in the skin, reduce the production of dopaquinone, and thus inhibit the production of melanin, achieving the effect of brightening the skin tone and reducing pigmentation. In addition, ascorbyl glucoside can also promote the production of collagen, help reduce wrinkles and improve skin roughness, showing a significant anti-aging effect. At the same time, it can also effectively resist the damage to the skin caused by ultraviolet radiation, reduce the production of free radicals, and protect the skin from oxidative invasion.

[0003] However, despite the many advantages of ascorbyl glucoside, its color stability is still a problem that needs to be solved in practical applications. Due to the influence of environmental factors (such as light, temperature, humidity, etc.), the color of ascorbyl glucoside may change, which not only affects its appearance, but also may affect its use effect in cosmetics.

[0004] Therefore, developing a composition capable of stabilizing the color of ascorbyl glucoside is of great significance for improving its application effect in antioxidant skin care products. Summary of the invention

[0005] In view of the deficiencies of the prior art, the present invention aims to provide a composition for inhibiting the discoloration of ascorbyl glucoside and its application in antioxidant skin care products, wherein the composition can effectively inhibit the discoloration of ascorbyl glucoside over time and maintain its good appearance. At the same time, the composition can further enhance the antioxidant properties of ascorbyl glucoside, so that it can play a better role in skin care products.

[0006] In order to achieve the above object, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention provides a composition for inhibiting discoloration of ascorbyl glucoside, wherein the composition comprises the following components in parts by mass:

[0008]

[0009]

[0010] Preferably, the composition comprises the following components in parts by mass:

[0011]

[0012] In a second aspect, the present invention provides use of the composition described in the first aspect in preparing a skin care product with whitening and / or anti-oxidation effects.

[0013] In a third aspect, the present invention provides a whitening and antioxidant essence, wherein the essence comprises the composition for inhibiting discoloration of ascorbyl glucoside according to the first aspect.

[0014] Preferably, the composition is added to the essence in an amount of 0.5-1.0 wt %.

[0015] More preferably, the essence also contains a moisturizer, a preservative, a pH adjuster, and a solvent.

[0016] More preferably, the moisturizing agent is selected from at least one of glycerol, allantoin, 1,3-propylene glycol, butylene glycol, and 1,2-hexanediol;

[0017] The preservative is selected from p-hydroxyacetophenone;

[0018] The pH regulator is selected from arginine;

[0019] The solvent is selected from deionized water.

[0020] In a fourth aspect, the present invention provides a method for preparing the whitening and antioxidant essence according to the third aspect, comprising the following steps:

[0021] S1. Mix the moisturizer and deionized water, heat to 55°C, stir at 200 rpm for 20 min, and keep warm for later use to obtain component A;

[0022] S2. The preservative and component A were mixed at 55°C and stirred at 200 rpm for 5 min to obtain component B;

[0023] S3. Cool component B to 40°C, add the composition for inhibiting discoloration of ascorbic acid glucoside at a rotation speed of 500 rpm, continue stirring and mixing for 10 minutes, and finally add a pH adjuster to adjust the pH to 6.5-7.0, stop stirring, and discharge the material to obtain the whitening antioxidant essence.

[0024] In the present invention:

[0025] Ascorbyl glucoside is a water-soluble vitamin C derivative with similar antioxidant activity to vitamin C. It can scavenge free radicals and prevent oxidation reactions. It has better stability than vitamin C, but it is still easily oxidized and causes discoloration.

[0026] The extract of Matsutake has excellent antioxidant effect, which contains a variety of antioxidant ingredients such as Matsutake polysaccharides, polyphenols and flavonoids, which can effectively remove free radicals and inhibit oxidation reactions. In addition, the extract of Matsutake also has a certain antibacterial effect, which can further improve the stability of the overall formula.

[0027] Tiger orchid root extract is rich in various antioxidant ingredients, such as polyphenols and alkaloid compounds, which can effectively scavenge free radicals and protect cells from oxidative damage. At the same time, tiger orchid root extract also has certain antibacterial effects, which can further improve the stability of the overall formula.

[0028] Astaxanthin is a carotenoid with strong antioxidant activity. The conjugated double bonds in its structure can effectively scavenge free radicals and prevent the occurrence of oxidation reactions. In addition, astaxanthin is lipophilic and hydrophilic, and can scavenge free radicals from the cell surface and the inside of the phospholipid membrane, and can more effectively penetrate the skin to exert its antioxidant effect.

[0029] The present invention has found that ascorbyl glucoside, Tricholoma matsutake extract, Cycas tiglerae root extract and astaxanthin all have strong antioxidant activity, and they can work together to inhibit the occurrence of oxidation reactions and improve the stability of the formula.

[0030] Beneficial effects of the present invention:

[0031] 1. The composition provided by the present invention has good stability when used in combination with ascorbyl glucoside, Tricholoma matsutake extract, Cycas cycad root extract and astaxanthin, effectively inhibits the discoloration of ascorbyl glucoside over time, and enhances the antioxidant property of ascorbyl glucoside;

[0032] 2. The whitening and antioxidant essence provided by the present invention has excellent antioxidant properties and tyrosinase activity inhibition properties, and can effectively reduce the production of melanin, thereby achieving a whitening effect. DETAILED DESCRIPTION

[0033] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0034] Unless otherwise specified, the test methods used in the specific implementation methods are all conventional methods; the materials, reagents, etc. used, unless otherwise specified, can be obtained from commercial channels; the percentages mentioned in the specific implementation methods, unless otherwise specified, are all mass percentages.

[0035] In the present invention:

[0036] The Tricholoma matsutake extract is a Tricholoma matsutake extract, the INCI name is TRICHOLOMA MATSUTAKE EXTRACT, purchased from Yunnan Yingge Biotechnology Co., Ltd., specification 10:1;

[0037] Cymbidium grandiflorum root extract is Cymbidium grandiflorum root extract, INCI name is CYMBIDIUM GRANDIFLORUM ROOT EXTRACT, purchased from Yunnan Yingge Biotechnology Co., Ltd., specification 20:1;

[0038] Astaxanthin: INCI name is ASTAXANTHIN, water-soluble astaxanthin prepared according to the preparation method of Example 1 in Chinese patent CN111793014B;

[0039] Ascorbyl glucoside: INCI name ASCORBYL GLUCOSIDE, purchased from Cosfa International Trading (Guangzhou) Co., Ltd.

[0040] Other raw materials are commercially available.

[0041] Preparation of the composition:

[0042] The raw materials were accurately weighed according to the mass ratio in Table 1, and the weighed raw materials were placed in a mixer and mixed evenly to obtain a composition for inhibiting the discoloration of ascorbyl glucoside.

[0043] Table 1 Raw materials and their mass fractions of the composition

[0044] Raw materials\group Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 Composition 7 Composition 8 Ascorbyl Glucoside 10 13 15 13 13 13 13 21 Tricholoma matsutake extract 2 3 4 - 4.8 4 - - Cynarostemon Root Extract 2 3 5 4.8 - 4 - - Astaxanthin 1 2 3 3.2 3.2 - 8 -

[0045] Note: “-” in the table means no addition.

[0046] Performance Test:

[0047] 1. Stability test

[0048] The compositions 1 to 8 were diluted with solvent to a concentration of 1 wt % of the test sample, and the following tests were performed, wherein the solvent was deionized water.

[0049] High temperature test: The constant temperature incubator was adjusted to 45±1℃. The test samples prepared above were placed in three transparent glass bottles with a sample volume of 20mL / bottle. After sealing, they were placed in a constant temperature incubator. After 3 months, they were taken out and returned to room temperature. The appearance changes were observed. The test results are shown in Table 2.

[0050] Room temperature test: The test samples prepared above were placed in three transparent glass bottles, with a sample volume of 20 mL / bottle. After sealing, the bottles were placed at room temperature for 3 months, and the changes in the appearance of the samples were observed. The test results are shown in Table 2.

[0051] Lighting test: A lighting device was installed 20-30cm above the experimental table for testing. The lighting conditions were set to LED light (light color 6000k) and light illumination (300lx) to meet the test requirements. The test samples prepared above were placed in three transparent glass bottles, with a sample volume of 20mL / bottle. After sealing, they were placed under the lighting conditions. After 3 months, they were taken out and returned to room temperature. The appearance changes were observed. The test results are shown in Table 2.

[0052] Table 2 Stability test results

[0053] Group\Test Item 45±1℃, place for 3 months Tested at room temperature, placed for 3 months Light test, 3 months Test Results Composition 1 constant constant constant Stablize Composition 2 constant constant constant Stablize Composition 3 constant constant constant Stablize Composition 4 Darkening Darkening Darkening Unstable Composition 5 Darkening Darkening Darkening Unstable Composition 6 Darkening Darkening Darkening Unstable Composition 7 Darkening Darkening Darkening Unstable Composition 8 Darkening Darkening Darkening Unstable

[0054] Note: The test sample is a colorless to orange transparent solution before being placed. If the color turns yellow or darker after being placed, it is unstable.

[0055] Result analysis:

[0056] It can be seen from the test results in Table 2 that under different temperature tests, the colors of the five groups of compositions 4-8 all changed, while the colors of the three groups of compositions 1-3 did not change, and the properties of compositions 1-3 were more stable than those of compositions 4-8, proving that ascorbyl glucoside, Tricholoma matsutake extract, Cycas tiglerae root extract and astaxanthin have good stability when compounded in the ratio provided by the present invention. After being placed at 45±1°C for 3 months, at room temperature, and under light for 3 months, the color and appearance of the composition for inhibiting the discoloration of ascorbyl glucoside provided by the present invention did not change. When the composition lacked any one or more of Tricholoma matsutake extract, Cycas tiglerae root extract and astaxanthin, the stability of the composition deteriorated, further proving that the composition has a significant effect on inhibiting the discoloration of ascorbyl glucoside.

[0057] 2. Antioxidant performance test

[0058] Before placement: Take a solvent to dilute the compositions 1-8 that have not been tested at room temperature to a concentration of 0.0001 wt % of the test sample, and perform the following tests, wherein the solvent is deionized water.

[0059] After standing: Compositions 1-8 after standing for 3 months at room temperature were further diluted with a solvent to a concentration of 0.0001 wt % of the test sample, and the following tests were performed, wherein the solvent was deionized water.

[0060] 2.1 Determination of DPPH free radical scavenging ability:

[0061] DPPH was prepared with anhydrous ethanol to 2×10 -4 mol / L DPPH solution, take several portions of 2mL of test sample, DPPH solution, and anhydrous ethanol, mix 2mL of test sample and 2mL of DPPH solution and place at room temperature for 30 minutes, and measure the absorbance at a wavelength of 517nm to obtain Ai; mix 2mL of test sample and 2mL of anhydrous ethanol and measure the absorbance according to the above method as Aj; mix 2mL of DPPH solution and 2mL of anhydrous ethanol and measure the absorbance according to the above method as Ac. Each sample is measured in parallel 3 times and the average value is taken.

[0062] The scavenging rate of DPPH free radicals of each test sample was calculated according to the following formula (1) and recorded in the following Table 3.

[0063] Clearance (%) = (1-(Ai-Aj) / Ac) × 100% ........... (1)

[0064] Wherein: Ai is the absorbance of the mixture of 2 mL test sample + 2 mL DPPH solution; Aj is the absorbance of the mixture of 2 mL test sample + 2 mL anhydrous ethanol; Ac is the absorbance of the mixture of 2 mL DPPH solution + 2 mL anhydrous ethanol.

[0065] 2.2 Determination of superoxide free radical O2- scavenging ability:

[0066] Test sample absorbance test: accurately aspirate 1 mL of the test sample, add 4.5 mL of 50 mmol / L Tris-HCl buffer with a pH value of 8.2, and then mix well. Keep it in a constant temperature water bath at 25°C for 20 minutes, then take it out, immediately add 0.3 mL of 25 mmol / L pyrogallol solution, shake it quickly, and react it in a constant temperature water bath at 25°C for 5 minutes, then add 1 mL of 8 mmol / L HCl, mix well, and terminate the reaction. Measure the absorbance at 325 nm.

[0067] Blank control group absorbance test: Perform the above operation using distilled water instead of the test sample.

[0068] Each group of samples was measured three times in parallel and the average value was taken.

[0069] The clearance rate was calculated according to formula (2) and recorded in Table 3 below.

[0070] Clearance rate (%) = (A0-A1) / A0×100% ........... (2)

[0071] Where: A0 is the average absorbance of the blank; A1 is the average absorbance of the test sample.

[0072] 2.3 Determination of hydroxyl free radical scavenging ability:

[0073] Add 1mL 0.15mmol / L FeSO4, 0.4mL 2mmol / L salicylic acid, 0.2mL test sample, 1mL 6mmol / L H2O2 and 0.4mL distilled water into the test tube to prepare the sample as the test sample group; use distilled water instead of the above test sample to prepare the sample as the blank group; use distilled water instead of the above salicylic acid to prepare the sample as the control group; react at 37℃ for 30 minutes; each sample is measured in parallel 3 times and the average value is taken.

[0074] The hydroxyl radical scavenging capacity was calculated according to formula (3) and recorded in Table 3 below.

[0075] Clearance rate (%) = (A 空白 -(A 样品 -A 对照 )) / A 空白 ×100%........(3)

[0076] Table 3 Antioxidant performance test results

[0077]

[0078]

[0079] Result analysis:

[0080] According to the test results in Table 3, after storage, the antioxidant properties of the five groups of compositions 4-8 showed a more obvious decline, while the antioxidant properties of the three groups of compositions 1-3 only declined slightly, indicating that the ascorbic acid glucoside, Tricholoma matsutake extract, Cycas tiglerae root extract and astaxanthin of the present invention have good stability when used in combination. When any one or more of Tricholoma matsutake extract, Cycas tiglerae root extract and astaxanthin are missing, the antioxidant properties of the composition will deteriorate over time.

[0081] Preparation of application examples:

[0082] Weigh the raw materials according to Table 4;

[0083] S1. Mix the moisturizer and deionized water, heat to 55°C, stir at 200 rpm for 20 min, and keep warm for later use to obtain component A;

[0084] S2. The preservative and component A were mixed at 55°C and stirred at 200 rpm for 5 min to obtain component B;

[0085] S3. Component B is cooled to 40°C, the composition is added at a rotation speed of 500 rpm, and stirring and mixing is continued for 10 minutes. Finally, a pH adjuster is added to adjust the pH to 6.5, stirring is stopped, and the material is discharged to obtain an essence.

[0086] Table 4 Mass percentage of raw materials in application examples

[0087]

[0088] Note: “-” in the table means no addition.

[0089] Application example whitening effect test:

[0090] Test environment conditions: In a stable indoor environment, the indoor temperature is 24℃-27℃, and the relative humidity is 40%-50%.

[0091] Tested substances: Essences of Application Examples 1-3. All essences had been sealed and left to stand at room temperature for 3 months before the test and were used directly.

[0092] Subjects: Volunteers aged 30±2 years with similar skin color ITA values ​​were selected.

[0093] Test plan: Each test substance was tested on 5 volunteers, and the test part of the volunteers was the face. The multifunctional skin tester (model: MPA580) produced by Courage Khazaka of Germany was used to measure the initial ITA value of each group of volunteers. Then, the test substances of application examples 1 to 3 were used respectively, with the dosage of 10 ml at a time, and the application method was twice a day for 4 weeks. The ITA value of each group of volunteers was tested (the average value was taken), and the whitening effect of the test substance was evaluated. The higher the ITA value, the better the whitening effect. The test results are shown in Table 5 below.

[0094] Table 5 ITA value test results of application examples 1-3

[0095]

[0096] Result analysis:

[0097] According to the results in Table 5, the whitening effects of the test substances in Application Examples 1-3 show a certain positive correlation with the concentration of Composition 2. The higher the concentration of Composition 2, the better the whitening effect of the essence, which has a certain concentration dependence. At the same time, by comparing Application Examples 1-3, it can be seen that the test substance in Application Example 3 has obviously better whitening effect, which further proves that the composition of ascorbyl glucoside, Tricholoma matsutake extract, Cycas tiglerae root extract and astaxanthin provided by the present invention can maintain the stability of ascorbyl glucoside well when used in combination in this ratio, so that the product maintains a good skin care effect.

[0098] The relationship between tyrosinase and whitening is mainly reflected in the synthesis process of melanin. Tyrosinase is a key enzyme in the synthesis of melanin. It catalyzes the conversion of tyrosine into dopa, which is then further oxidized to form dopaquinone, and finally forms melanin. The formation of melanin is the main reason for darkening of the skin. Therefore, the composition of ascorbyl glucoside, Tricholoma matsutake extract, Cyperus tigrinus root extract and astaxanthin provided by the present invention has excellent antioxidant and tyrosinase activity inhibition effects, and can effectively reduce the generation of melanin, thereby achieving a whitening effect.

[0099] Finally, it should be noted that the above embodiments and comparative examples are only used to illustrate the technical solution of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for inhibiting discoloration of ascorbyl glucoside, characterized in that: The composition comprises the following components in parts by mass:

2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by mass:

3. Use of the composition according to claim 1 or 2 in the preparation of a skin care product with whitening and / or anti-oxidation effects.

4. A whitening and antioxidant essence, characterized in that: The essence contains the composition for inhibiting discoloration of ascorbyl glucoside according to claim 1 or 2.

5. The whitening and antioxidant essence according to claim 4, characterized in that: The composition is added in an amount of 0.5-1.0 wt % in the essence.

6. The whitening and antioxidant essence according to claim 4 or 5, characterized in that: The essence also contains a moisturizer, a preservative, a pH regulator and a solvent.

7. The whitening and antioxidant essence according to claim 6, characterized in that: The moisturizing agent is selected from at least one of glycerin, allantoin, 1,3-propylene glycol, butylene glycol, and 1,2-hexanediol; The preservative is selected from p-hydroxyacetophenone; The pH regulator is selected from arginine; The solvent is selected from deionized water.

8. The method for preparing the whitening and antioxidant essence according to claim 7, characterized in that: The following steps are involved: S1. Mix the moisturizer and deionized water, heat to 55°C, stir at 200 rpm for 20 min, and keep warm for later use to obtain component A; S2. The preservative and component A were mixed at 55°C and stirred at 200 rpm for 5 min to obtain component B; S3. Cool component B to 40°C, add the composition for inhibiting discoloration of ascorbic acid glucoside at a rotation speed of 500 rpm, continue stirring and mixing for 10 minutes, and finally add a pH adjuster to adjust the pH to 6.5-7.0, stop stirring, and discharge the material to obtain the whitening antioxidant essence.

Images