Convection heating system for sterilization and control method

Abstract

The invention discloses a convection heating system for sterilization and a control method. The convection heating system comprises a hose, a peristaltic pump, a constant-temperature water bath kettle, an ice-water bath container, a stirring device and a thermocouple, the peristaltic pump is used for conveying original bacterial liquid, the constant-temperature water bath kettle is used for heating the original bacterial liquid, the ice-water bath container contains an ice-water mixture and is used for cooling the original bacterial liquid, and the bacterial liquid container is placed above the stirring device and is used for stirring and mixing the original bacterial liquid; a sterile test tube is placed in an ice-water bath container, a hose is wound on a stainless steel frame at equal intervals, the head end of the hose is connected to a peristaltic pump and then connected into a bacterial liquid container containing original bacterial liquid, then the hose is completely soaked in a constant-temperature water bath kettle, and finally the tail end of the hose is connected into the sterile test tube; the thermocouple is connected inside the hose and used for measuring the temperature of liquid inside the hose. By adopting the convection heating system, the heating rate can be increased, and the sterilization time and the sterilization temperature can be accurately controlled.

Description

Technical Field

[0001] The present invention belongs to the technical field of sterilization heating devices, and in particular relates to a convection heating system for sterilization and a control method. Background Art

[0002] In the traditional sterilization method of original bacterial liquid, glass test tubes are usually used for heating. However, this method has obvious limitations: the thick wall of the glass tube leads to low heat conduction efficiency and cannot ensure uniform heating; at the same time, since temperature errors are easily generated during the heat transfer process, it is difficult to accurately control the heating time and temperature, which not only affects the sterilization effect, but also poses a challenge to the accuracy and repeatability of the experimental results; in addition, the traditional method has more manual operations, which increases the risk of contamination, and its work efficiency is low for large-scale sample processing.

[0003] Based on this, the applicant has developed a more efficient, accurate and safe sterilization convection heating system to solve the above technical problems. Summary of the invention

[0004] The present invention provides a convection heating system and control method for sterilization, which solve the technical problems of low heating rate and large temperature error caused by heat transfer in the existing heating sterilization method.

[0005] The present invention provides a convection heating system for sterilization, which adopts the following technical scheme: comprising a hose, a peristaltic pump, a constant temperature water bath, and an ice water bath container; the peristaltic pump is used to transport the original bacterial liquid, the constant temperature water bath is used to heat the original bacterial liquid, and the ice water bath container contains an ice-water mixture for cooling the original bacterial liquid;

[0006] A sterile test tube is placed in the ice water bath container, the hose is equidistantly wound on a stainless steel frame, the first end of the hose is connected to a peristaltic pump, and then connected to the inside of a bacterial liquid container containing the original bacterial liquid, then the hose is completely immersed in a constant temperature water bath, and finally the end of the hose is connected to the inside of the sterile test tube.

[0007] Preferably, the heating system further comprises a stirring device, and a bacterial liquid container is placed above the stirring device for stirring and mixing the original bacterial liquid.

[0008] Preferably, the heating system further comprises a thermocouple, which is connected to the inside of the hose and is used to measure the temperature of the liquid inside the hose.

[0009] Preferably, the stirring device is a magnetic stirrer.

[0010] The present invention also provides a control method for a convection heating system for sterilization, the control method comprising the steps of controlling the thermal sterilization time:

[0011] S1. Before heat sterilization, clean the hose with sterile PBS and empty the liquid;

[0012] S2. Wrap the hose around the stainless steel frame at equal intervals, connect the first end of the hose to the peristaltic pump, and then connect it to the inside of the bacterial liquid container containing the original bacterial liquid, and then completely immerse the hose in a constant temperature water bath;

[0013] S3. The original bacteria liquid is sucked into the hose through a peristaltic pump. When the length of the hose remains unchanged, the sterilization time can be changed by changing the flow rate of the original bacteria liquid sucked by the peristaltic pump. The sterilization time and accuracy are calculated based on the flow rate of the original bacteria liquid sucked, the radius of the hose, and the length of the hose.

[0014] Preferably, the sterilization time is calculated according to the following formula:

[0015]

[0016] Where t is time, q is flow rate, r is hose radius, and l is hose length.

[0017] Preferably, the accuracy of the sterilization time is calculated according to the following formula:

[0018]

[0019] Preferably, when a certain volume of original bacterial liquid is discharged, the original bacterial liquid is connected to a sterile test tube placed in an ice water bath container at the end of the hose to terminate the sterilization; after each experiment is completed, the hose is cleaned and emptied with 75% alcohol for next use.

[0020] Preferably, the control method also includes a step of controlling the thermal sterilization temperature: placing a hose with a pre-marked length in a 60°C constant temperature water bath, inserting the temperature sensing wire of the thermocouple into the hose, changing the length of the hose, changing the heating distance of the original bacteria liquid, calculating the heating time, and recording the real-time temperature of the original bacteria liquid inside the hose.

[0021] Beneficial effects of the present invention:

[0022] (1) Improve heating rate and sterilization efficiency: The use of convection heating can significantly improve the heating rate, allowing the original bacterial solution to reach the sterilization temperature in a short time; at the same time, by accurately controlling the flow rate of the peristaltic pump, the sterilization time can be accurately controlled, thereby improving the sterilization efficiency.

[0023] (2) Precise control of sterilization temperature: The introduction of thermocouples enables real-time monitoring of the temperature of the liquid inside the hose, thereby achieving precise control of the sterilization temperature, helping to reduce temperature errors and improve sterilization effects.

[0024] (3) Strong adaptability: The system can adapt to sterilization requirements of different flow rates, different lengths of hoses, and different temperatures; by adjusting system parameters, sterilization treatment of different types of original bacterial liquids can be achieved.

[0025] (4) Easy to clean and maintain: The hoses and peristaltic pumps and other key components are easy to disassemble and clean, which facilitates cleaning and disinfection after each experiment, ensuring the hygiene and reliability of the system. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] For ease of explanation, the present invention is described in detail with reference to the following specific implementations and the accompanying drawings.

[0027] Figure 1 It is a structural schematic diagram of the heating system of this embodiment;

[0028] Figure 2 The temperature of the original bacterial solution at different flow rates in this embodiment changes at different times.

[0029] In the figure:

[0030] 1- bacterial liquid container; 2- magnetic stirrer; 3- peristaltic pump; 4- hose; 5- thermocouple; 6- constant temperature water bath; 7- ice water bath container. DETAILED DESCRIPTION

[0031] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solutions of the present invention, but the present invention is not limited to these embodiments; in the following description, specific details such as specific configurations and components are provided only to help fully understand the embodiments of the present invention. Therefore, it should be clear to those skilled in the art that various changes and modifications can be made to the embodiments described herein without departing from the scope and spirit of the present invention. In addition, for the sake of clarity and brevity, the description of known functions and structures is omitted.

[0032] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features of the embodiments may be combined with each other.

[0033] like Figure 1-Figure 2As shown, a convection heating system for sterilization provided by the present embodiment comprises a hose 4, a peristaltic pump 3, a constant temperature water bath 6, and an ice-water bath container 7; the peristaltic pump 3 is used to transport the original bacterial solution, the constant temperature water bath 6 is used to heat the original bacterial solution, and the ice-water bath container 7 contains an ice-water mixture, which is used to cool the original bacterial solution and terminate the sterilization; a sterile test tube is placed in the ice-water bath container 7, and a hose 4 with a length of 10 m is firstly wound around a stainless steel frame at equal intervals, and the first end of the hose 4 is connected to the peristaltic pump 3, and then connected to the interior of the bacterial solution container 1 containing the original bacterial solution, and then the 9.24 m hose 4 is completely immersed in the constant temperature water bath 6, and the original bacterial solution is adsorbed by the hose 4 through the operation of the peristaltic pump 3, and transported to the hose 4 in the constant temperature water bath 6 for heating, and the flow rate of the peristaltic pump 3 is changed so that the sterilization time can be accurately controlled. After the sterilization is completed, the end of the hose 4 is connected to the interior of the sterile test tube, and the sterilization process is terminated by the ice-water mixture in the ice-water bath container 7.

[0034] Furthermore, the heating system also includes a stirring device. Specifically, the stirring device can be a magnetic stirrer 2. A bacterial liquid container 1 is placed above the stirring device to stir and mix the original bacterial liquid.

[0035] Furthermore, the heating system also includes a thermocouple 5, and a temperature sensing wire of the thermocouple 5 is inserted into the hose 4 to measure the temperature of the liquid inside the hose.

[0036] This embodiment also provides a control method for a convection heating system for sterilization, including a step of controlling a thermal sterilization time and a step of controlling a thermal sterilization temperature;

[0037] Among them, the control steps of thermal sterilization time include:

[0038] S1. Before heat sterilization, clean the hose with sterile PBS and empty the liquid;

[0039] S2. The hose 4 is equidistantly wound around the stainless steel frame, the first end of the hose 4 is connected to the peristaltic pump 3, and then connected to the inside of the bacterial liquid container 1 containing the original bacterial liquid, and then the hose is completely immersed in a constant temperature water bath 6;

[0040] S3. The original bacteria liquid is sucked into the hose through the peristaltic pump 3. When the length of the hose is fixed, the sterilization time can be changed by changing the flow rate of the original bacteria liquid sucked by the peristaltic pump 3. The sterilization time and accuracy are calculated based on the flow rate of the sucked original bacteria liquid, the radius of the hose, and the length of the hose.

[0041]

[0042] According to formula ①, the transformation can be obtained:

[0043] Where t is time (min), q is flow rate (μL / min), and V is hose volume (mm 3), r is the hose radius (mm), 1 is the hose length (mm); by formula ②, the sterilization time can be calculated.

[0044] According to formula ②, we can get: lnt=lnπ+2lnr+lnl-lnq------Formula ③

[0045] According to formula ③, we can get the following derivative:

[0046] The accuracy of the sterilization time can be calculated by formula ④; after many experiments, it can be concluded that the accuracy of the sterilization time can reach 0.01%.

[0047] S4. When a certain volume of original bacterial liquid is discharged, the original bacterial liquid is connected to a sterile test tube placed in an ice water bath container 7 at the end of the hose 4 to achieve the purpose of stopping sterilization; after each experiment is completed, the hose is cleaned and emptied with 75% alcohol for next use.

[0048] Among them, the control step of thermal sterilization temperature is as follows: place the hose 4 with a pre-marked length in a 60°C constant temperature water bath 6, insert the temperature sensing wire of the thermocouple 5 into the hose, change the heating distance of the original bacterial liquid by changing the length of the hose, calculate the heating time, and record the real-time temperature of the original bacterial liquid inside the hose, and obtain the changes in the temperature rise process of the bacterial liquid inside the hose at different flow rates (1450μL / min, 725μL / min, 362.5μL / min), such as Figure 2 shown.

[0049] After the heating time is 0.10min, the inside of the hose with different flow rates can reach the set sterilization temperature, and the temperature change difference of different flow rates is not large, and changing the flow rate will not have a significant impact on the sterilization effect; the temperature accuracy can be calculated by the ratio of the minimum scale of the thermocouple 0.01℃ and the measured temperature 60℃, with an accuracy of 0.02%. Therefore, the convection heating system can accurately control the sterilization temperature.

[0050] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0051] In the description of the present application, it should be understood that the terms "upper", "lower", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

Claims

1. A convection heating system for sterilization, characterized in that: It comprises a hose (4), a peristaltic pump (3), a constant temperature water bath (6), and an ice water bath container (7); the peristaltic pump (3) is used to transport the original bacterial liquid, the constant temperature water bath (6) is used to heat the original bacterial liquid, and the ice water bath container (7) contains an ice-water mixture for cooling the original bacterial liquid; A sterile test tube is placed in the ice water bath container (7), the hose (4) is equidistantly wound on a stainless steel frame, the head end of the hose (4) is connected to a peristaltic pump (3), and then connected to the inside of a bacterial liquid container (1) containing the original bacterial liquid, and then the hose is completely immersed in a constant temperature water bath pot (6), and finally the end of the hose (4) is connected to the inside of the sterile test tube.

2. A convection heating system for sterilization according to claim 1, characterized in that: The heating system also includes a stirring device, and a bacterial liquid container (1) is placed above the stirring device for stirring and mixing the original bacterial liquid.

3. A convection heating system for sterilization according to claim 1, characterized in that: The heating system further comprises a thermocouple (5), which is connected to the inside of the hose (4) and is used to measure the temperature of the liquid inside the hose.

4. A convection heating system for sterilization according to claim 2, characterized in that: The stirring device is a magnetic stirrer (2).

5. A control method for a convection heating system for sterilization, characterized in that: The control method comprises the steps of controlling the thermal sterilization time: S1. Before heat sterilization, clean the hose with sterile PBS and empty the liquid; S2. Wrap the hose (4) equidistantly around the stainless steel frame, connect the first end of the hose (4) to the peristaltic pump (3), and then connect it to the inside of the bacterial liquid container (1) containing the original bacterial liquid, and then completely immerse the hose in a constant temperature water bath (6); S3. The bacterial liquid is sucked into the hose (4) through the peristaltic pump (3). When the length of the hose is fixed, the sterilization time can be changed by changing the flow rate of the bacterial liquid sucked by the peristaltic pump. The sterilization time and accuracy are calculated based on the flow rate of the bacterial liquid sucked, the radius of the hose, and the length of the hose.

6. A control method for a sterilization convection heating system according to claim 5, characterized in that: Calculate the sterilization time according to the following formula: Where t is time, q is flow rate, r is hose radius, and 1 is hose length.

7. A control method for a sterilization convection heating system according to claim 6, characterized in that: The accuracy of sterilization time is calculated according to the following formula:

8. The control method of a sterilization convection heating system according to claim 5, characterized in that: When a certain volume of the original bacterial liquid is discharged, the original bacterial liquid is connected to a sterile test tube placed in an ice water bath container (7) at the end of the hose (4) to achieve the purpose of terminating the sterilization; After each experiment, clean and drain the hose with 75% alcohol for next use.

9. The control method of a sterilization convection heating system according to claim 5, characterized in that: The control method also includes a step of controlling the thermal sterilization temperature: placing a hose (4) with a pre-marked length in a 60°C constant temperature water bath (6), inserting a temperature sensing line of a thermocouple (5) into the hose, changing the length of the hose to change the heating distance of the original bacteria liquid, calculating the heating time, and recording the real-time temperature of the original bacteria liquid in the hose.

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