Small-molecule milk peptide and preparation method and device thereof

Abstract

The invention belongs to the technical field of milk peptides, and particularly relates to a preparation method of small molecular milk peptides, which comprises the following steps: by taking liquid containing dairy products or a mixture of liquid containing dairy products and solid powder containing dairy products as raw materials, carrying out multiple times of enzymolysis on the raw materials and an adaptive enzymolysis agent in a multi-stage enzymolysis device, so that the enzymolysis reaction is more sufficient; the temperature during the enzymolysis reaction is variable, stirring is continuously performed at the stirring speed of 8-35 rpm during the variable temperature period, the molecular weight distribution of the prepared small-molecular milk peptide finished product is 1-2000 Da, the proportion exceeds 90%, the molecular weight is small, and compared with milk or milk powder, the small-molecular milk peptide finished product is more beneficial to human body absorption.

Description

Technical Field

[0001] The invention belongs to the technical field of milk peptides, and in particular relates to a small molecule milk peptide and a preparation method and device thereof. Background Art

[0002] The main components of milk or milk powder include water, protein, fat, lactose, minerals, vitamins and other ingredients. Protein includes casein, whey protein, lactalbumin and lactoglobulin. However, the molecular weight of milk and milk powder is relatively large, generally ranging from 14,000 Daltons (Da) to 250,000 Daltons (Da), which is not conducive to human absorption. Summary of the invention

[0003] In view of this, the present invention provides a small molecule milk peptide and a preparation method and device thereof, so as to solve the technical problem in the prior art that the molecular weight of milk or milk powder is relatively large and is not conducive to human absorption.

[0004] To achieve the above objectives, this application adopts the following scheme:

[0005] A method for preparing small molecule milk peptides, comprising an S10 enzymatic hydrolysis step, wherein the enzymatic hydrolysis step comprises:

[0006] S11: obtaining a pre-treated raw material for standby use, wherein the raw material may be a liquid containing a dairy product or a mixture of a liquid containing a dairy product and a solid powder containing a dairy product;

[0007] S12: screening suitable enzymolysis agents for standby use, wherein the enzymolysis agents include a first enzymolysis agent, a second enzymolysis agent, a third enzymolysis agent, a fourth enzymolysis agent and an Nth enzymolysis agent;

[0008] S13: putting the raw material and the enzymolysis agent into a multi-stage enzymolysis device for multiple enzymolysis. After the last enzymolysis, a preliminary product of small molecule milk peptide is obtained:

[0009] Among them, the enzymatic hydrolysis includes temperature change treatment and stirring treatment, and the temperature change treatment includes: first heating to a first preset temperature and keeping warm for a preset time, then cooling to a second preset temperature and keeping warm for a preset time, and then heating to a third preset temperature and keeping warm for a preset time; the stirring rate during the stirring treatment is 8rpm to 35rpm.

[0010] Preferably, in step S11, the liquid containing dairy products is milk, and the solid powder containing dairy products is milk powder.

[0011] Preferably, in step S12, the first enzymolytic agent is a protease that degrades casein, the second enzymolytic agent is a protease that degrades whey protein, lactalbumin, and lactoglobulin, the third enzymolytic agent is an enzyme that degrades lactose, and the fourth enzymolytic agent is lipase.

[0012] Preferably, in step S13, the first preset temperature is 45°C to 65°C, the second preset temperature is 30°C to 42°C, the third preset temperature is 44°C to 50°C, and the insulation time is 23 min to 45 min.

[0013] Preferably, in the step S13, when the multiple enzymolysis is performed, the number of enzymolysis is four times, and the number of enzymolysis is four times, and specifically includes the following steps:

[0014] S131: putting the raw material and the casein-degrading protease into the multi-stage enzymatic hydrolysis device for primary enzymatic hydrolysis to obtain an enzymatic hydrolysis product A, wherein the mass ratio of the raw material to the casein-degrading protease is 100:(1 to 2);

[0015] S132: adding the enzymatic hydrolysis product A and the protease for degrading whey protein, lactalbumin and lactoglobulin into the multi-stage enzymatic hydrolysis device for secondary enzymatic hydrolysis to obtain an enzymatic hydrolysis product B, wherein the mass ratio of the enzymatic hydrolysis product A to the protease for degrading whey protein, lactalbumin and lactoglobulin is 100:(1 to 1.5);

[0016] S133: adding the enzymatic hydrolysis product B and the lactose-degrading enzyme into the multi-stage enzymatic hydrolysis device for three-stage enzymatic hydrolysis to obtain an enzymatic hydrolysis product C, wherein the mass ratio of the enzymatic hydrolysis product B to the lactose-degrading enzyme is 100:(0.5 to 1);

[0017] S134: putting the enzymatic hydrolysis product C and the lipase into the multi-stage enzymatic hydrolysis device for four-stage enzymatic hydrolysis to obtain the initial small molecule milk peptide product, and the mass ratio of the enzymatic hydrolysis product C to the lipase is 100:(0.5 to 0.8).

[0018] Preferably, the step S134 further includes: obtaining collagen liquid, mixing the collagen liquid with the small molecule milk peptide primary product in a mass ratio of (3:97) to (5:95), spray-drying, and obtaining a small molecule milk peptide finished product.

[0019] Preferably, the method further includes a drying step S20, wherein the drying step includes spraying and drying the small molecule milk peptide preliminary product to obtain a small molecule milk peptide finished product.

[0020] A small molecule milk peptide is prepared by the small molecule milk peptide preparation method described above.

[0021] A small molecule milk peptide preparation device, comprising a drying device, and also comprising a multi-stage enzymolysis device as described in the small molecule milk peptide preparation method described above, wherein the multi-stage enzymolysis device comprises at least N groups of enzymolysis components and a corresponding number of enzyme inactivation components, and the enzymolysis components are connected to the enzyme inactivation components; the enzymolysis components are used for enzymolysis reaction, and the enzyme inactivation components are used to inactivate enzymes to prevent enzymes of various levels of enzymolysis reactions from mixing with each other, wherein N≥4;

[0022] The enzymolysis component comprises an enzymolysis reaction tank, an enzyme material input part, a discharge switching part and a discharge pipe, wherein the enzyme material input part is installed at the top of the enzymolysis reaction tank, the discharge switching part is provided with an input end and two switchable output ends, the input end of the discharge switching part is connected to the output end of the enzymolysis reaction tank, and the two output ends are respectively connected to the discharge pipe and the enzyme inactivation component;

[0023] The enzyme inactivation component includes an enzyme inactivation input pipe, an enzyme inactivation output pipe, a filter element and a heating enzyme inactivation element. One end of the enzyme inactivation input pipe is connected to each discharge switching element, and the other end is connected to the input end of the filter element. One end of the enzyme inactivation output pipe is connected to the output end of the filter element, and the other end is connected to the top of each enzymatic reaction tank. The heating enzyme inactivation element is installed on the enzyme inactivation input pipe.

[0024] Preferably, the multi-stage enzymatic hydrolysis device also includes at least N groups of stirring components with the same structure and N groups of temperature control components with the same structure, and each of the stirring components and each of the temperature control components are correspondingly installed on each of the enzymatic hydrolysis components, each of the stirring components is used to stir the reactants in each of the enzymatic hydrolysis reaction tanks, and each of the temperature control components is used to heat, cool and keep the reactants in each of the enzymatic hydrolysis reaction tanks warm.

[0025] The technical solution adopted in this application can achieve the following beneficial effects:

[0026] The present invention provides a method for preparing small molecule milk peptides. The method adopts a liquid containing dairy products or a mixture of a liquid containing dairy products and a solid powder containing dairy products as a raw material. The raw material and an adaptive enzymolysis agent are subjected to multiple enzymolysis in a multi-stage enzymolysis device to make the enzymolysis reaction more complete. The temperature during the enzymolysis reaction is variable. During the variable temperature period, the product is continuously stirred at a stirring rate of 8 rpm to 35 rpm. The molecular weight distribution of the prepared small molecule milk peptide product is more than 90% in the range of 1 Da to 2000 Da. The molecular weight is relatively small, which is more conducive to human absorption than milk or milk powder.

[0027] The small molecule milk peptide provided by the present invention has a smaller molecular weight than the milk peptide in the prior art and is more conducive to human absorption; and the present application also provides a small molecule milk peptide, which has a smaller molecular weight and not only has the efficacy and function of milk peptide, but also has the efficacy and function of collagen peptide.

[0028] The present invention provides a small molecule milk peptide preparation device, which is provided with at least N groups of enzymatic hydrolysis components and enzyme inactivation components, wherein the feed ends of the enzyme inactivation components are respectively connected to the discharge ends of the enzymatic hydrolysis components, and the discharge ends are respectively connected to the feed ends of the enzymatic hydrolysis components; the enzymatic hydrolysis components are used to respectively carry out enzymatic hydrolysis reactions at various levels, and the enzyme inactivation components are used to inactivate enzymes to prevent the enzymes of the enzymatic hydrolysis reactions at various levels from mixing with each other, and can make the enzymatic hydrolysis reactions more sufficient. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is the molecular weight chromatogram of finished product 1 in Example 1 of the present application.

[0030] Figure 2 This is the molecular weight chromatogram of finished product 2 in Example 2 of the present application.

[0031] Figure 3 This is the molecular weight chromatogram of finished product three in comparative example 1 of the present application.

[0032] Figure 4 This is the molecular weight chromatogram of finished product 4 in Comparative Example 2 of the present application.

[0033] Figure 5 This is the molecular weight chromatogram of finished product 5 in Example 3 of the present application.

[0034] Figure 6 This is the molecular weight chromatogram of finished product six in Example 3 of the present application.

[0035] Figure 7 This is the molecular weight chromatogram of finished product seven in Example 4 of the present application.

[0036] Figure 8 This is an oblique bird's-eye view of the multi-stage enzymatic hydrolysis device of the present application.

[0037] Fig. 9 This is an oblique bird's-eye view of an enzymatic hydrolysis component in the multi-stage enzymatic hydrolysis device of the present application.

[0038] Fig.10 It is a front view of the enzyme inactivation component of the present application.

[0039] Fig.11 This is a top view of the multi-stage enzymatic hydrolysis device of the present application.

[0040] Fig.12 This is a cross-sectional view of an enzymatic hydrolysis component in the multi-stage enzymatic hydrolysis device of the present application.

[0041] In the figure: a multi-stage enzymolysis device 10, an enzymolysis component 20, an enzymolysis reaction tank 21, an enzyme material input part 22, a discharge switching part 23, a discharge pipe 24, a sampling part 25, an enzyme inactivation component 30, an enzyme inactivation input pipe 31, an enzyme inactivation output pipe 32, a filter part 33, a heating enzyme inactivation part 34, a substrate input component 40, an input pipe 41, an input amount detection part 42, a positive pressure component 50, a positive pressure fan 51, a positive pressure air hole 52, a stirring component 60, a stirring motor 61, a stirring rod 62, an upper stirring blade 63, a lower stirring blade 64, a temperature control component 70, a temperature control tube 71, a heating component 72, a cooling component 73, and a heat preservation shell 74. DETAILED DESCRIPTION

[0042] In order to facilitate the understanding of the present application, the present application will be described more comprehensively below in conjunction with the accompanying drawings. And the preferred implementation of the present application is given. However, the present application can be implemented in many different forms and is not limited to the implementation described herein. On the contrary, the purpose of providing these implementations is to make the disclosure of the present application more thoroughly and comprehensively understood.

[0043] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0044] A method for preparing small molecule milk peptides, comprising an S10 enzymatic hydrolysis step, wherein the enzymatic hydrolysis step comprises:

[0045] S11: obtaining a pre-treated raw material for standby use, wherein the raw material may be a liquid containing a dairy product or a mixture of a liquid containing a dairy product and a solid powder containing a dairy product;

[0046] S12: screening suitable enzymolysis agents for standby use, wherein the enzymolysis agents include a first enzymolysis agent, a second enzymolysis agent, a third enzymolysis agent, a fourth enzymolysis agent and an Nth enzymolysis agent;

[0047] S13: putting the raw material and the enzymolysis agent into a multi-stage enzymolysis device for multiple enzymolysis. After the last enzymolysis, a preliminary product of small molecule milk peptide is obtained:

[0048] Among them, the enzymatic hydrolysis includes temperature change treatment and stirring treatment, and the temperature change treatment includes: first heating to a first preset temperature and keeping warm for a preset time, then cooling to a second preset temperature and keeping warm for a preset time, and then heating to a third preset temperature and keeping warm for a preset time; the stirring rate during the stirring treatment is 8rpm to 35rpm.

[0049] In this embodiment, a pretreated liquid containing dairy products or a mixture of a liquid containing dairy products and a solid powder is used as a raw material to prepare small molecule peptides through enzymolysis; the pretreatment includes sterilization, cooling, etc.; during the enzymolysis, multiple enzymolysis is performed by a multi-stage enzymolysis device (in stages), first, the preliminary enzymolysis (the first enzymolysis) hydrolyzes the macromolecular protein into smaller peptide segments, and then, other adaptive enzymolysis agents are selected to further enzymolyze the product after the preliminary enzymolysis to obtain peptides with lower molecular weight and higher biological activity, and the number of enzymolysis can be selected according to actual conditions. In this embodiment, the number of enzymolysis is not less than 4 times; the enzymolysis agents include a first enzymolysis agent, a second enzymolysis agent, a third enzymolysis agent, a fourth enzymolysis agent and an Nth enzymolysis agent, the first enzymolysis agent is used for the first enzymolysis, the second enzymolysis agent is used for the second enzymolysis, and so on, which are not listed here. It should be noted that the enzymolysis agents for each enzymolysis can be one or more, which are specifically selected by those skilled in the art according to actual conditions.

[0050] During the preparation, first obtain a pretreated liquid containing dairy products or a mixture of a liquid containing dairy products and solid powder, for example: use milk as a raw material or use a mixture of milk and milk powder as a raw material. When the raw material is a mixture of milk and milk powder, the ratio of the milk to the milk powder can be 1: (0.5 to 1); then, put the selected enzymolysis agent (the first enzymolysis agent) into the multi-stage enzymolysis device, heat it to a first preset temperature and keep it warm for a preset time, then cool it to a second preset temperature and keep it warm for a preset time, and then heat it to a third preset temperature and keep it warm for a preset time. During this period, continue to stir at a low speed, and the stirring rate is 8rpm to 35rpm; after the last insulation, the initial product of the small molecule milk peptide is obtained.

[0051] A liquid containing dairy products or a mixture of a liquid containing dairy products and a solid powder containing dairy products is used as a raw material, and the raw material and an adaptive enzymolysis agent are subjected to multiple enzymolysis in a multi-stage enzymolysis device to make the enzymolysis reaction more complete. The temperature during the enzymolysis reaction is variable, and the product is continuously stirred at a stirring rate of 8 rpm to 35 rpm during the variable temperature period. The prepared small molecule milk peptide finished product has a smaller molecular weight and is more conducive to human absorption.

[0052] In addition, after the enzymatic hydrolysis is completed in this embodiment, in order to prevent the remaining enzymatic agent from continuing to hydrolyze, an enzyme inactivation treatment is required. Specifically, the enzyme inactivation treatment can be performed once after each enzymatic hydrolysis reaction. The enzyme inactivation treatment process can adopt an ultra-high temperature resin enzyme inactivation process for enzyme inactivation treatment. The enzymatic agent is passed into the enzyme inactivation component 30 and exposed to a temperature of 135°C for 2s to 4s. It should be noted that: this method is not limited to the use of this enzyme inactivation process, and other enzyme inactivation processes existing in the prior art can achieve the same effect.

[0053] The milk production in Ningxia Hui Autonomous Region continues to grow. This application uses milk as raw material, which can solve the problem of milk surplus on the one hand. On the other hand, the protein in the milk is enzymatically hydrolyzed to produce peptides, which can not only retain the original nutrients but also produce more biologically active peptides, such as casein phosphopeptides, immune active peptides, antioxidant peptides, ACE inhibitory peptides, etc. These peptides have multiple health benefits.

[0054] In some specific embodiments, in the step S12, the first enzymolytic agent is a protease that degrades casein, the second enzymolytic agent is a protease that degrades whey protein, lactalbumin, and lactoglobulin, the third enzymolytic agent is an enzyme that degrades lactose, and the fourth enzymolytic agent is a lipase. In this embodiment, the first enzymolytic agent is a protease that degrades casein and the second enzymolytic agent is a protease that degrades whey protein, lactalbumin, and lactoglobulin for the purpose of: reducing the molecular weight of protein in milk or milk powder, which is more conducive to human absorption; the third enzymolytic agent is selected to be an enzyme that degrades lactose for the purpose of: because the data of the "National Lactose Intolerance Survey" pointed out that there are about 310 million people in China who are lactose intolerant, and nearly 350 million people are suspected of being lactose intolerant, totaling up to 660 million people. Lactose intolerant people are mainly because of the lack or insufficient activity of lactase in the body, and milk contains a large amount of lactose. When people with lactose intolerance eat Gastrointestinal discomfort and skin problems may occur, which may lead to lactose malabsorption, causing symptoms such as intestinal rumbling, abdominal pain, gas production, osmotic diarrhea, etc. Therefore, in this embodiment, when the third enzymolytic agent is an enzyme that degrades lactose, the third enzymolytic agent is used to degrade the lactose in milk or milk powder, so that the lactose in the milk or milk powder is decomposed by the human body in time; the purpose of selecting lipase as the fourth enzymolytic agent is to hydrolyze the milk fat in milk or milk powder by lipase to obtain the main component with frankincense flavor, so that the prepared small molecule milk peptide has a stronger frankincense flavor, and the optimal conditions for preparing small molecule milk peptides by enzymolyzing the milk fat in milk or milk powder with lipase include: temperature change treatment and stirring treatment, and the temperature change treatment includes: first heating to a first preset temperature and keeping warm for a predetermined time, then cooling to a second preset temperature and keeping warm for a predetermined time, and then heating to a third preset temperature and keeping warm for a predetermined time; the stirring rate during the stirring treatment is 8rpm to 35rpm. The small molecule milk peptide prepared by the method provided in this embodiment has a small molecular weight and is easily absorbed by the human body. The lactose in the small molecule milk peptide is degraded and can be absorbed by the human body in time. The small molecule milk peptide has a strong milk flavor and a better taste.

[0055] In order to accelerate the enzymatic reaction rate and control enzyme denaturation to obtain small molecule milk peptides, in some specific embodiments, in the S13 step, the first preset temperature is 45°C to 65°C, the second preset temperature is 30°C to 42°C, the third preset temperature is 44°C to 50°C, and the insulation time is 23min to 45min.

[0056] As the temperature rises, the movement of enzyme molecules accelerates, the binding rate of substrate and enzyme increases, thereby accelerating the rate of enzymatic reaction. Although increasing the temperature is conducive to accelerating the reaction rate, too high a temperature will cause the enzyme protein to denature and inactivate, thereby reducing the reaction rate. Therefore, the first preset temperature is 45°C to 65°C, the second preset temperature is 30°C to 42°C, and the third preset temperature is 44°C to 50°C.

[0057] In some preferred embodiments, in the step S13, when the multiple enzymolysis is performed, the number of enzymolysis is four times, and the number of enzymolysis is four times, and specifically includes the following steps:

[0058] S131: putting the raw material and the casein-degrading protease into the multi-stage enzymatic hydrolysis device for primary enzymatic hydrolysis to obtain an enzymatic hydrolysis product A, wherein the mass ratio of the raw material to the casein-degrading protease is 100:(1 to 2);

[0059] S132: adding the enzymatic hydrolysis product A and the protease for degrading whey protein, lactalbumin and lactoglobulin into the multi-stage enzymatic hydrolysis device for secondary enzymatic hydrolysis to obtain an enzymatic hydrolysis product B, wherein the mass ratio of the enzymatic hydrolysis product A to the protease for degrading whey protein, lactalbumin and lactoglobulin is 100:(1 to 1.5);

[0060] S133: adding the enzymatic hydrolysis product B and the lactose-degrading enzyme into the multi-stage enzymatic hydrolysis device for three-stage enzymatic hydrolysis to obtain an enzymatic hydrolysis product C, wherein the mass ratio of the enzymatic hydrolysis product B to the lactose-degrading enzyme is 100:(0.5 to 1);

[0061] S134: putting the enzymatic hydrolysis product C and the lipase into the multi-stage enzymatic hydrolysis device for four-stage enzymatic hydrolysis to obtain the initial small molecule milk peptide product, and the mass ratio of the enzymatic hydrolysis product C to the lipase is 100:(0.5 to 0.8).

[0062] It is proved by the following examples that when the number of enzymolysis is four times, and the enzymolytic agent is the protease that degrades casein in the first enzymolysis, the enzymolytic agent is the protease that degrades whey protein, lactalbumin, and lactoglobulin in the second enzymolysis, the enzymolytic agent is the enzyme that degrades lactose in the third enzymolysis, and the enzymolytic agent is lipase in the fourth enzymolysis, the molecular weight distribution of the prepared small molecule milk peptide finished product is more than 90% in the range of 1Da to 2000Da, the molecular weight is small, and it is more conducive to human absorption than milk or milk powder.

[0063] Furthermore, the step S134 also includes: obtaining collagen liquid, mixing the collagen liquid with the small molecule milk peptide primary product in a mass ratio of (3:97) to (5:95), spraying and drying, and obtaining a small molecule milk peptide finished product.

[0064] In this embodiment, first, the raw material (milk or milk and milk powder) and the enzymolysis agent are enzymolyzed four times by a multi-stage enzymolysis device to obtain a small molecule milk peptide primary product; then, the collagen solution is mixed with the small molecule milk peptide primary product to obtain a mixture, and the mixture is powdered and dried to obtain a small molecule milk peptide finished product; It should be noted that: According to the "Requirements for Organic Product Production, Processing, Labeling and Management System" issued by the State Administration for Market Regulation and the Standardization Administration of China on September 2, 2019, "the organic ingredient content is equal to or higher than According to the regulations that only products with an organic content of more than 95% and certified as organic products can be labeled "organic" before the product name, the collagen solution and the small molecule milk peptide preliminary product are mixed in a mass ratio of (3:97) to (5:95) and sprayed with powder and dried to obtain a small molecule milk peptide finished product, which is an organic small molecule milk peptide finished product. However, if the method provided in the present application does not produce organic small molecule milk peptides, the collagen solution and the small molecule milk peptide preliminary product can also be mixed in a mass ratio of (50:50) to (10:90) and sprayed with powder and dried to obtain a small molecule milk peptide finished product.

[0065] It should also be noted that the preparation method of the collagen liquid is the same as the preparation method of the small molecule milk peptide preliminary product. Both use the raw materials for preparing the collagen liquid and the enzymatic agent to carry out multiple enzymatic hydrolysis reactions in a multi-stage enzymatic hydrolysis device to obtain the collagen liquid. Finally, the collagen liquid and the small molecule milk peptide preliminary product are mixed and dried. Compared with directly mixing the raw materials for preparing the small molecule milk peptide preliminary product with the raw materials for preparing the collagen liquid and then carrying out enzymatic hydrolysis, this can avoid the problem of insufficient reaction or excessive reaction during enzymatic hydrolysis due to the large difference in the molecular weight of the raw materials.

[0066] In this embodiment, the prepared small molecule milk peptide product not only has the efficacy and function of milk peptide, but also has the efficacy and function of collagen peptide, and has a smaller molecular weight, which is more conducive to human absorption.

[0067] In some specific embodiments, a drying step S20 is also included, wherein the drying step includes spraying and drying the small molecule milk peptide preliminary product to obtain a small molecule milk peptide finished product.

[0068] A small molecule milk peptide is prepared by the small molecule milk peptide preparation method described above.

[0069] The small molecule milk peptide provided by the present invention has a smaller molecular weight than the molecular weight of milk peptide in the prior art, and is more conducive to human absorption; and the present application also provides a small molecule milk peptide, which not only has the efficacy and function of milk peptide, but also has the efficacy and function of collagen peptide, and has a smaller molecular weight, which is more conducive to human absorption.

[0070] Please refer to Figure 8 A small molecule milk peptide preparation device comprises a drying device and a multi-stage enzymolysis device as described in the small molecule milk peptide preparation method as described above, wherein the multi-stage enzymolysis device comprises at least N groups of enzymolysis components 20 and enzyme inactivation components 30, wherein the feed end of the enzyme inactivation component 30 is respectively connected to the discharge end of each enzymolysis component 20, and the discharge end is respectively connected to the feed end of each enzymolysis component 20; each enzymolysis component 20 is used to perform enzymolysis reactions at different levels respectively, and the enzyme inactivation component 30 is used to inactivate the enzyme to prevent the enzymes of the enzymolysis reactions at different levels from mixing; thus, a first-stage enzymolysis reaction is first performed in one enzymolysis component 20, and then the enzymolysis product is sent to the enzyme inactivation component 30, and the enzyme mixed in the enzymolysis product is inactivated and then sent to another enzymolysis component 20 for a second-stage enzymolysis reaction; the steps of the third-stage enzymolysis reaction to the Nth-stage enzymolysis reaction are the same as above, thereby separating the enzymes of the enzymolysis reactions at different levels to prevent mutual inhibition between different enzymes, thereby improving the production efficiency of the finished product.

[0071] Please refer to Fig. 9 Furthermore, each enzymolysis component 20 includes an enzymolysis reaction tank 21, an enzyme material input part 22, a discharge switching part 23 and a discharge pipe 24. The enzyme material input part 22 is installed at the top of the enzymolysis reaction tank 21. The discharge switching part 23 is provided with an input end and two switchable output ends. The input end of the discharge switching part 23 is connected to the output end of the enzymolysis reaction tank 21, and the two output ends are respectively connected to the discharge pipe 24 and the enzyme inactivation component 30; the enzyme material input part 22 is used to feed the enzyme required for the enzymolysis reaction into the enzymolysis reaction tank 21, the enzymolysis reaction tank 21 is used to perform the enzymolysis reaction, the discharge switching part 23 is used to send the product of the enzymolysis reaction to the discharge pipe 24 or the enzyme inactivation component 30 through a switching operation, and the discharge pipe 24 is used to output the final finished reaction product. Specifically, when the next level of enzymolysis reaction is still needed, the product of the enzymolysis reaction is sent to the enzyme inactivation component 30; when the next level of enzymolysis reaction is not needed, the product of the enzymolysis reaction is sent out from the discharge pipe 24.

[0072] In this embodiment, the enzyme feeder 22 realizes quantitative enzyme feed input through a spiral driving structure arranged in the pipe, and the spiral driving feeding method can break up the lumps in the enzyme feed to prevent the pipe from being blocked.

[0073] In this embodiment, the discharge switching element 23 selects an electric three-way valve or an electric four-way valve according to demand.

[0074] In this embodiment, a sampling piece 25 is provided on the outside of each enzymatic hydrolysis reaction tank 21 for sampling and inspection from the enzymatic hydrolysis reaction tank 21, so as to facilitate the staff to check whether the products of each level of enzymatic hydrolysis reaction meet the requirements. Specifically, the sampling piece 25 is a pipeline with a valve, such as a faucet.

[0075] Please refer to Fig.10 Further, the enzyme inactivation component 30 includes an enzyme inactivation input pipe 31, an enzyme inactivation output pipe 32, a filter element 33 and a heating enzyme inactivation element 34, one end of the enzyme inactivation input pipe 31 is connected to each discharge switching element 23, and the other end is connected to the input end of the filter element 33, one end of the enzyme inactivation output pipe 31 is connected to the output end of the filter element 33, and the other end is connected to the top of each enzymolysis reaction tank 21, and the heating enzyme inactivation element 34 is installed on the enzyme inactivation input pipe 31; the enzyme inactivation input pipe 31 is used to send the enzymolysis product of each enzymolysis reaction tank 21 into the filter element 33, the heating enzyme inactivation element 34 is used to inactivate the enzyme in the enzyme inactivation input pipe 31 by heating, the filter element 33 is used to filter out the inactivated enzyme from the enzymolysis product, thereby improving the purity of each level of enzymolysis product, and the enzyme inactivation output pipe 32 is used to send the enzymolysis product after enzyme inactivation to each enzymolysis reaction tank 21 to participate in the next level of enzymolysis reaction.

[0076] Please refer to Fig. 9 Furthermore, the multi-stage enzymatic hydrolysis device 10 also includes a substrate input component 40, which includes an input tube 41 and an input quantity detection component 42. The discharge end of the input tube 41 is installed at the top of any enzymatic hydrolysis reaction tank 21, and the input quantity detection component 42 is installed on the input tube 41; the input tube 41 is used to input the substrate into the enzymatic hydrolysis reaction tank 21, and the input quantity detection component 42 is used to obtain the volume data of the substrate input from the input tube into the enzymatic hydrolysis reaction tank.

[0077] Please refer to Fig.11 Furthermore, the multi-stage enzymatic hydrolysis device 10 also includes a positive pressure component 50, which includes a positive pressure fan 51 and a positive pressure air hole 52 opened at the top of each enzymatic hydrolysis reaction tank 21, and the air outlet end of the positive pressure fan 51 is respectively connected to the top of each enzymatic hydrolysis reaction tank 21; the positive pressure fan 51 is used to blow sterile gas into each enzymatic hydrolysis reaction tank 21, and the positive pressure air hole 52 is used to exhaust gas to the outside, thereby adjusting the air pressure in each enzymatic hydrolysis reaction tank 21, so that the air pressure in each enzymatic hydrolysis reaction tank 21 meets the requirements of the enzymatic hydrolysis reaction to be carried out.

[0078] Please refer to Fig.12 Further, the multi-stage enzymolysis device 10 also includes at least N groups of stirring components 60 with the same structure, each stirring component 60 is correspondingly installed on each enzymolysis component, each stirring component 60 includes a stirring motor 61, a stirring rod 62, a plurality of upper stirring blades 63 with the same structure and a plurality of lower stirring blades 64 with the same structure, the fixed end of the stirring motor 61 is installed at the top of the enzymolysis reaction tank 21, one end of the stirring rod 62 is drivingly connected to the driving end of the stirring motor 61, and the other end is rotatably connected to the bottom of the enzymolysis reaction tank 21, each upper stirring blade 63 is fixedly installed on the stirring rod 62 and is located in the middle of the enzymolysis reaction tank 21 to stir the substrate and enzyme material and accelerate the enzymolysis reaction, each lower stirring blade 64 is fixedly installed on the stirring rod 62 and is located at the bottom of the enzymolysis reaction tank 21; the bottom end of each lower stirring blade 64 is fan-shaped to be close to the tank wall at the bottom of the enzymolysis reaction tank 21, so as to stir the bottom of the enzymolysis reaction tank 21 during rotation to prevent the substrate and enzyme material from sticking to the bottom of the tank.

[0079] Please refer to Fig.12 Furthermore, the multi-stage enzymolysis device 10 further includes N groups of temperature control components 70 with the same structure, each temperature control component 70 is correspondingly installed on each enzymolysis component 20, each temperature control component 70 includes a temperature control tube 71, a heating element 72, a cooling element 73 and a heat preservation shell 74, the temperature control tube 71 is wound around the tank body of the enzymolysis reaction tank 21, the top of the temperature control tube 71 is respectively connected to the output end of the heating element 72 and the input end of the cooling element 73, and the bottom end of the temperature control tube 71 is respectively connected to the heating element 72 The input end is connected to the output end of the cooling component 73, the insulation shell 74 is arranged on the outside of the tank body of the enzymolysis reaction tank 21, and covers the temperature control tube 71; the temperature control tube 71 is used to heat up or cool down the enzymolysis reaction tank 21, the heating component 72 is used to provide a heat source for the temperature control tube 71, the cooling component 73 is used to provide a cold source for the temperature control tube 71, and the insulation shell 74 is used to isolate the temperature control tube 71 from the outside world, so as to control the temperature in each enzymolysis reaction tank 21 to meet the requirements of the enzymolysis reaction to be carried out.

[0080] Furthermore, each enzymatic hydrolysis reaction tank 21 is provided with a temperature detection component and a pressure detection component. The temperature detection component is used to collect temperature data in each enzymatic hydrolysis reaction tank, and the pressure detection component is used to collect air pressure data in each enzymatic hydrolysis reaction tank.

[0081] Furthermore, the multi-stage enzymatic hydrolysis device also includes a control terminal, which is respectively communicatively connected with the dosage detection component, the pressure detection component and the temperature detection component to upload the data obtained by the dosage detection component, the pressure detection component and the temperature detection component to the control terminal; the control terminal is respectively electrically connected with the enzyme material input component 22, the discharge switching component 23, the positive pressure fan 51, the stirring motor 61, the heating component 72 and the cooling component 73 to control the start and stop of the enzyme material input component 22, the discharge switching component 23, the positive pressure fan 51, the stirring motor 61, the heating component 72 and the cooling component 73.

[0082] In one embodiment, the control terminal includes a display and an input component. The display is used to display the data obtained by the dosage detection component, each pressure detection component and each temperature detection component. The input component is a device that can input data, for example, a keyboard. The operator inputs data from the input component according to the data displayed on the display, thereby controlling the enzymatic hydrolysis reaction in each enzymatic hydrolysis reaction tank 21. Specifically, by controlling each enzyme material input component 22, the amount of enzyme material added to each enzymatic hydrolysis reaction tank 21 is adjusted; by controlling the output direction of each discharge switching component 23, the output direction of the enzymatic hydrolysis product in each enzymatic hydrolysis reaction tank 21 is switched; by controlling the wind force of the positive pressure fan 51, the air pressure in each enzymatic hydrolysis reaction tank 21 is adjusted; by controlling the rotation speed of each stirring motor 61, the stirring speed in each enzymatic hydrolysis reaction tank 21 is adjusted; by controlling the output of each heating component 72 and each cooling component 73, the temperature in each enzymatic hydrolysis reaction tank 21 is adjusted.

[0083] In one embodiment, the control terminal controls each enzyme material input component 22, each material discharging switching component 23, the positive pressure fan 51, each stirring motor 61, each heating component 72 and each cooling component 73 according to preset parameters, so as to adjust the amount of enzyme material introduced into the enzymatic hydrolysis reaction tank 21 by each enzyme material input component 22 according to the substrate volume data uploaded by the dosage detection component 42; controls the material discharging switching component 23 to output the enzymatic hydrolysis product or send it to the enzyme inactivation component 30 for the next enzymatic hydrolysis reaction according to the number of enzymatic hydrolysis reactions required for the finished product; controls the positive pressure fan 51 to adjust the air pressure in each enzymatic hydrolysis reaction tank 21 according to the air pressure data uploaded by each pressure detection component; controls the rotation speed of each stirring motor 61 according to the requirements of the enzymatic hydrolysis reaction; controls each heating component 72 and the cooling component 73 to adjust the temperature in each enzymatic hydrolysis reaction tank 21 according to the temperature data uploaded by each temperature detection component.

[0084] In this embodiment, the control terminal is integrated on the positive pressure fan 51 .

[0085] Example 1: Performing multi-stage enzymatic hydrolysis reaction by increasing the number of enzymatic hydrolysis components 20

[0086] 1. The number of enzymatic hydrolysis components 20 is set according to the enzymatic hydrolysis reaction level required for product processing, and these enzymatic hydrolysis components 20 are sequentially a first enzymatic hydrolysis component, a second enzymatic hydrolysis component, ..., an Nth enzymatic hydrolysis component;

[0087] 2. Adding substrate into the enzymolysis reaction tank 21 of the first enzymolysis component through the substrate input part, and adding enzyme material into the enzymolysis reaction tank 21 of the first enzymolysis component according to the amount of substrate added;

[0088] 3. After the enzymatic hydrolysis reaction in the first enzymatic hydrolysis component is completed, the enzymatic hydrolysis product is sent to the enzyme inactivation component 30, the enzyme material is inactivated by the heat inactivation component, and the inactivated enzyme material is separated from the enzymatic hydrolysis product by the filter element 33;

[0089] 4. Send the treated enzymolysis product to the enzymolysis reaction tank 21 of the second enzymolysis component, and add enzyme material to the enzymolysis reaction tank 21 of the second enzymolysis component according to the amount of substrate added;

[0090] 5. After the enzymatic hydrolysis reaction in the second enzymatic hydrolysis component is completed, the enzymatic hydrolysis product is sent to the enzyme inactivation component 30, the enzyme material is inactivated by the heat inactivation component, and the inactivated enzyme material is separated from the enzymatic hydrolysis product by the filter element 33;

[0091] 6. Repeat the above steps until the product after N-stage enzymatic hydrolysis reaction is obtained.

[0092] Example 2. Performing multi-stage enzymolysis reaction by increasing the number of enzyme material input parts 22

[0093] In this embodiment, only two enzymatic hydrolysis components 20 are needed, and the two enzymatic hydrolysis components 20 are named as the first enzymatic hydrolysis component and the second enzymatic hydrolysis component respectively;

[0094] 1. According to the enzymatic reaction order required for product processing, enzyme material input parts 22 are respectively arranged on the first enzymatic hydrolysis component and the second enzymatic hydrolysis component, and they are named as the first enzyme material input part 22, the second enzyme material input part 22, ..., the Nth enzyme material input part 22 in the order of enzyme material input;

[0095] 2. The substrate is introduced into the enzymolysis reaction tank 21 of the first enzymolysis component through the substrate introduction part, and the first enzyme material introduction part 22 introduces enzyme material into the enzymolysis reaction tank 21 of the first enzymolysis component according to the introduction amount of the substrate;

[0096] 3. After the enzymatic hydrolysis reaction in the first enzymatic hydrolysis component is completed, the enzymatic hydrolysis product is sent to the enzyme inactivation component 30, the enzyme material is inactivated by the heat inactivation component, and the inactivated enzyme material is separated from the enzymatic hydrolysis product by the filter element 33;

[0097] 4. The treated enzymolysis product is sent to the enzymolysis reaction tank 21 of the second enzymolysis component, and the second enzyme material input part 22 inputs enzyme material into the enzymolysis reaction tank 21 of the second enzymolysis component according to the input amount of the substrate;

[0098] 5. After the enzymatic hydrolysis reaction in the second enzymatic hydrolysis component is completed, the enzymatic hydrolysis product is sent to the enzyme inactivation component 30, the enzyme material is inactivated by the heat inactivation component, and the inactivated enzyme material is separated from the enzymatic hydrolysis product by the filter element 33;

[0099] 6. The treated enzymolysis product is sent to the enzymolysis reaction tank 21 of the first enzymolysis component, and the third enzyme material input part 22 inputs enzyme material into the enzymolysis reaction tank 21 of the second enzymolysis component according to the input amount of the substrate;

[0100] 7. Repeat the above process until the product after N-level enzymatic hydrolysis reaction is obtained.

[0101] The following specific experimental examples are used to further illustrate the technical solutions and technical effects of the present invention. It should be noted that the following experimental examples are only for further explaining the present invention and do not limit the technical solutions of the present invention.

[0102] 1. Experimental Materials and Methods

[0103] 1.1. Experimental Materials

[0104] Milk, milk powder, protease that degrades casein, protease that degrades whey protein, lactalbumin, and lactoglobulin, enzyme that degrades lactose, and lipase.

[0105] 1.2 Experimental methods

[0106] The experiment was divided into 6 treatment groups, namely, Example 1, Example 2, Comparative Example 1, Comparative Example 2, Example 3, and Example 4. Different small molecule milk peptides were prepared by the 6 treatment groups, and their parameters were detected according to the following detection methods:

[0107] The molecular weight of the small molecule milk peptide is detected according to high performance liquid chromatography: HPLC separates peptides through a chromatographic column, and peptide molecules of different sizes have different distributions in the HPLC chromatographic column. The molecular weight of the peptide can be indirectly calculated by detecting the retention time of the peptide molecules and combining it with the standard curve.

[0108] 2. Example 1

[0109] 100 g of sterilized milk and 1.5 g of casein-degrading protease were put into a multi-stage enzymatic hydrolysis reaction device, and the multi-stage enzymatic hydrolysis reaction device was started to perform the first enzymatic hydrolysis. During the first enzymatic hydrolysis, the multi-stage enzymatic hydrolysis reaction device was first heated to a first preset temperature (55° C.) and kept warm for 35 min; then, the multi-stage enzymatic hydrolysis reaction device was cooled to a second preset temperature (36° C.) and kept warm for 35 min; next, the multi-stage enzymatic hydrolysis reaction device was heated to a third preset temperature (47° C.) and kept warm for 35 min; during this period, the reactants in the multi-stage enzymatic hydrolysis reaction device were continuously stirred at a stirring rate of 22 rpm. After the last heat preservation, an enzymatic hydrolysis product A was obtained;

[0110] 100 g of enzymolysis product A and 1.2 g of protease for degrading whey protein, lactalbumin and lactoglobulin are put into a multistage enzymolysis reaction device, and the multistage enzymolysis reaction device is started to perform a second enzymolysis, wherein the temperature and stirring rate of the temperature variable treatment during the second enzymolysis are the same as the temperature and stirring rate of the temperature variable treatment during the first enzymolysis, to obtain an enzymolysis product B;

[0111] 100 g of the enzymolysis product B and 1.2 g of the lactose-degrading enzyme are added to the multi-stage enzymolysis device for three-stage enzymolysis, and the temperature and stirring rate of the temperature-changing treatment during the third enzymolysis are the same as the temperature and stirring rate of the temperature-changing treatment during the first enzymolysis, to obtain an enzymolysis product C;

[0112] 100g of enzymatic hydrolysis product C and 0.7g of lipase are put into the multi-stage enzymatic hydrolysis device for four-stage enzymatic hydrolysis. The temperature and stirring rate of the variable temperature treatment during the fourth enzymatic hydrolysis are the same as the temperature and stirring rate of the variable temperature treatment during the first enzymatic hydrolysis, to obtain the initial product of the small molecule milk peptide.

[0113] The small molecule milk peptide preliminary product obtained in Example 1 was subjected to powder spray drying treatment to obtain a small molecule milk peptide finished product, which is marked as finished product 1 for ease of description;

[0114] The molecular weight of the finished product 1 was tested by high performance liquid chromatography. The test results are as follows: Figure 1 As shown:

[0115] from Figure 1 It reflects that the molecular weight range of the finished product one prepared in Example 1 is 100Da to 2000Da, accounting for 75.38%, of which the molecular weight range of the finished product one is 100Da to 1000Da, accounting for 59.9%, and the molecular weight range of the finished product one is 1Da to 100Da, accounting for 19.72%, indicating that the molecular weight distribution of the finished product one is more than 90% of 1Da to 2000Da. The small molecule milk peptide prepared by using milk as raw material in the method provided by the present invention has a smaller molecular weight, which is more conducive to human absorption than milk.

[0116] 3. Example 2

[0117] The difference between this embodiment 2 and the above embodiment 1 is that: the composition of the raw material is changed, and the raw material is a mixture of 62.5g of milk and 37.5g of milk powder, and the other steps are the same as those in the above embodiment 1;

[0118] The small molecule milk peptide preliminary product obtained in this comparative example 2 was subjected to powder spray drying treatment to obtain a small molecule milk peptide finished product, which is marked as finished product 2 for ease of description;

[0119] The molecular weight of the finished product 2 was tested by high performance liquid chromatography. The test results are as follows: Figure 2 shown.

[0120] from Figure 2It reflects that the molecular weight range of the finished product two prepared in Example 2 is 100Da to 2000Da, accounting for 77.04%, of which the molecular weight range of the finished product two is 100Da to 1000Da, accounting for 61.28%, and the molecular weight range of the finished product two is 4Da to 100Da, accounting for 15.85%, indicating that the molecular weight distribution of the finished product two is more than 90% between 4Da and 2000Da, and the molecular weight of the small molecule milk peptide prepared by using a mixture of milk and milk powder as raw materials in the method provided by the present invention is also small, which is more conducive to human absorption than milk or milk powder.

[0121] 4. Comparative Example 1

[0122] The difference between this comparative example 1 and the above-mentioned embodiment 1 is that: the temperature change treatment is performed during the first enzymolysis, the second enzymolysis, the third enzymolysis and the fourth enzymolysis, and the reactants in each stage of the multi-stage enzymolysis reaction device are not stirred during the temperature change treatment. After the last heat preservation is completed, the initial product of small molecule milk peptide is obtained, and the other steps are the same as those in the above-mentioned embodiment 1;

[0123] The small molecule milk peptide preliminary product obtained in this comparative example 1 was subjected to powder spray drying treatment to obtain a small molecule milk peptide finished product, which is marked as finished product three for ease of description;

[0124] The molecular weight of the above-mentioned finished product 3 was tested by high performance liquid chromatography. The test results are as follows: Figure 3 shown.

[0125] from Figure 3 It reflects that the molecular weight range of the finished product three prepared in this comparative example 1 is 78.92% in the range of 100Da to 2000Da, of which the molecular weight range of the finished product three is 28.65% in the range of 100Da to 1000Da, and in addition, the molecular weight range of the finished product three is 5.99% in the range of 1Da to 100Da, indicating that the reactants in each stage of the multi-stage enzymatic hydrolysis reaction device are not stirred during the temperature change treatment during multiple enzymatic hydrolysis, and the molecular weight distribution of the prepared finished product three is between 100Da and 1000Da, which accounts for a lower proportion in this range than the finished product one prepared by stirring treatment in Example 1.

[0126] 5. Comparative Example 2

[0127] The difference between this comparative example 2 and the above-mentioned embodiment 1 is that: no temperature change treatment is performed during the first enzymolysis, the second enzymolysis, the third enzymolysis and the fourth enzymolysis, and the first enzymolysis, the second enzymolysis, the third enzymolysis and the fourth enzymolysis are stirred at a stirring rate of 22 rpm for 140 min, and the initial product of small molecule milk peptide is obtained after the stirring is completed, and the other steps are the same as those in the above-mentioned embodiment 1;

[0128] The small molecule milk peptide preliminary product obtained in this comparative example 2 was subjected to powder spray drying treatment to obtain a small molecule milk peptide finished product, which is marked as finished product 4 for ease of description;

[0129] The molecular weight of the finished product 4 was tested by high performance liquid chromatography. The test results are as follows: Figure 4 shown.

[0130] from Figure 4 It reflects that the molecular weight range of the finished product four prepared in this comparative example 2 is 76.31% in the range of 100Da to 2000Da, of which the molecular weight range of the finished product four is 33.21% in the range of 100Da to 1000Da, and in addition, the molecular weight range of the finished product four is 4.09% in the range of 7Da to 100Da, indicating that when the temperature change treatment is not performed during the multiple enzymatic hydrolysis, the molecular weight distribution of the prepared finished product four is between 100Da and 1000Da, which accounts for a lower proportion in this range than the finished product one prepared by the temperature change treatment in Example 1.

[0131] 6. Example 3

[0132] The difference between this embodiment 3 and the above embodiment 1 is that the stirring rate is changed, and the other steps are the same as the steps in the above embodiment 1. The stirring rate is specifically shown in Table 1:

[0133] Table 1 Stirring rate table in Example 3

[0134]

[0135] The molecular weights of the finished products 5 and 6 were tested by high performance liquid chromatography. The test results were as follows: Figure 5 and Figure 6 As shown:

[0136] When the stirring rate is 8 rpm, Figure 5 The data in the figure show that the molecular weight range of the prepared product V is between 1Da and 2000Da, accounting for 92.04%; when the stirring rate is 35rpm, Figure 6 The data in the figure show that the molecular weight range of the prepared finished product VI is 88.85% in the range of 3Da to 2000Da; all of them are inferior to the effect of the finished product 1 prepared at a stirring rate of 22rpm in the above Example 1.

[0137] 7. Example 4

[0138] The difference between this embodiment 4 and the above embodiment 1 is that 5g of collagen solution (prepared by the same method as the preparation method of the milk peptide primary product) is mixed with 95g of the small molecule milk peptide primary product prepared in the above embodiment 1 and then dried. The other steps are the same as those in the above embodiment 1 to obtain a small molecule milk peptide finished product, which is marked as finished product seven for ease of description. The molecular weight of the finished product seven is detected by high performance liquid chromatography. The detection result is as follows Figure 7 As shown:

[0139] Depend on Figure 7 It can be seen from the data that when the collagen liquid is prepared by the enzymatic hydrolysis method (milk peptide) provided in the present application, and the collagen liquid is mixed with the small molecule milk peptide primary product, the molecular weight range of the finished product seven is 6Da to 2000Da, accounting for 83.47%, of which the molecular weight range of the finished product seven is 100Da to 1000Da, accounting for 54.62%, and in addition, the molecular weight range of the finished product seven is 6Da to 100Da, accounting for 20.09%, indicating that the molecular weight range of the finished product seven prepared by mixing the small molecule milk peptide primary product with the collagen liquid is 6Da to 2000Da, accounting for more than 80%, and its molecular weight is also small, which is easy to be absorbed by the human body, and the finished product seven not only has the efficacy and function of collagen peptides but also has the efficacy and function of milk peptides.

[0140] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or substitutions within the technical scope disclosed by the present invention, and these modifications or substitutions should be included in the protection scope of the present invention.

Claims

1. A method for preparing small molecule milk peptides, characterized in that: The method comprises an S10 enzymolysis step, wherein the enzymolysis step comprises: S11: obtaining a pre-treated raw material for standby use, wherein the raw material may be a liquid containing a dairy product or a mixture of a liquid containing a dairy product and a solid powder containing a dairy product; S12: screening suitable enzymolysis agents for standby use, wherein the enzymolysis agents include a first enzymolysis agent, a second enzymolysis agent, a third enzymolysis agent, a fourth enzymolysis agent and an Nth enzymolysis agent; S13: putting the raw material and the enzymolysis agent into a multi-stage enzymolysis device for multiple enzymolysis. After the last enzymolysis, a preliminary product of small molecule milk peptide is obtained: Among them, the enzymatic hydrolysis includes temperature change treatment and stirring treatment, and the temperature change treatment includes: first heating to a first preset temperature and keeping warm for a preset time, then cooling to a second preset temperature and keeping warm for a preset time, and then heating to a third preset temperature and keeping warm for a preset time; the stirring rate during the stirring treatment is 8 rpm to 35 rpm.

2. The method for preparing small molecule milk peptide according to claim 1, characterized in that: In step S11, the liquid containing dairy products is milk, and the solid powder containing dairy products is milk powder.

3. The method for preparing small molecule milk peptide according to claim 1, characterized in that: In step S12, the first enzymolytic agent is a protease that degrades casein, the second enzymolytic agent is a protease that degrades whey protein, lactalbumin, and lactoglobulin, the third enzymolytic agent is an enzyme that degrades lactose, and the fourth enzymolytic agent is lipase.

4. The method for preparing small molecule milk peptide according to claim 3, characterized in that: In the step S13, the first preset temperature is 45°C to 65°C, the second preset temperature is 30°C to 42°C, the third preset temperature is 44°C to 50°C, and the insulation time is 23 min to 45 min.

5. The method for preparing small molecule milk peptide according to claim 4, characterized in that: In the step S13, when the enzymatic hydrolysis is performed multiple times, the number of times of enzymatic hydrolysis is four times, and the number of times of enzymatic hydrolysis is four times, specifically comprising the following steps: S131: putting the raw material and the casein-degrading protease into the multi-stage enzymatic hydrolysis device for primary enzymatic hydrolysis to obtain an enzymatic hydrolysis product A, wherein the mass ratio of the raw material to the casein-degrading protease is 100:(1 to 2); S132: adding the enzymatic hydrolysis product A and the protease for degrading whey protein, lactalbumin and lactoglobulin into the multi-stage enzymatic hydrolysis device for secondary enzymatic hydrolysis to obtain an enzymatic hydrolysis product B, wherein the mass ratio of the enzymatic hydrolysis product A to the protease for degrading whey protein, lactalbumin and lactoglobulin is 100:(1 to 1.5); S133: adding the enzymatic hydrolysis product B and the lactose-degrading enzyme into the multi-stage enzymatic hydrolysis device for three-stage enzymatic hydrolysis to obtain an enzymatic hydrolysis product C, wherein the mass ratio of the enzymatic hydrolysis product B to the lactose-degrading enzyme is 100:(0.5 to 1); S134: putting the enzymatic hydrolysis product C and the lipase into the multi-stage enzymatic hydrolysis device for four-stage enzymatic hydrolysis to obtain the initial small molecule milk peptide product, wherein the mass ratio of the enzymatic hydrolysis product C to the lipase is 100:(0.5 to 0.8).

6. The method for preparing small molecule milk peptide according to claim 5, characterized in that: The step S134 also includes: obtaining collagen liquid, mixing the collagen liquid with the small molecule milk peptide primary product in a mass ratio of (3:97) to (5:95), spraying and drying, and obtaining a small molecule milk peptide finished product.

7. The method for preparing small molecule milk peptide according to claim 1, characterized in that: It also includes a S20 drying step, which includes spraying and drying the small molecule milk peptide preliminary product to obtain a small molecule milk peptide finished product.

8. A small molecule milk peptide, characterized in that: The small molecule milk peptide is prepared by the preparation method of any one of claims 1 to 7.

9. A small molecule milk peptide preparation device, comprising a drying device, characterized in that: It also includes the multi-stage enzymolysis device described in the method for preparing small molecule milk peptides according to any one of claims 1 to 7, wherein the multi-stage enzymolysis device includes at least N groups of enzymolysis components and a corresponding number of enzyme inactivation components, and the enzymolysis components are connected to the enzyme inactivation components; the enzymolysis components are used for enzymolysis reactions, and the enzyme inactivation components are used to inactivate enzymes to prevent enzymes of various levels of enzymolysis reactions from mixing with each other, wherein N≥4; The enzymolysis component comprises an enzymolysis reaction tank, an enzyme material input part, a discharge switching part and a discharge pipe, wherein the enzyme material input part is installed at the top of the enzymolysis reaction tank, the discharge switching part is provided with an input end and two switchable output ends, the input end of the discharge switching part is connected to the output end of the enzymolysis reaction tank, and the two output ends are respectively connected to the discharge pipe and the enzyme inactivation component; The enzyme inactivation component includes an enzyme inactivation input pipe, an enzyme inactivation output pipe, a filter element and a heating enzyme inactivation element. One end of the enzyme inactivation input pipe is connected to each discharge switching element, and the other end is connected to the input end of the filter element. One end of the enzyme inactivation output pipe is connected to the output end of the filter element, and the other end is connected to the top of each enzymatic reaction tank. The heating enzyme inactivation element is installed on the enzyme inactivation input pipe.

10. The small molecule milk peptide preparation device according to claim 1, characterized in that: The multi-stage enzymatic hydrolysis device also includes at least N groups of stirring components with the same structure and N groups of temperature control components with the same structure. Each stirring component and each temperature control component are correspondingly installed on each enzymatic hydrolysis component. Each stirring component is used to stir the reactants in each enzymatic hydrolysis reaction tank, and each temperature control component is used to heat, cool and keep the reactants in each enzymatic hydrolysis reaction tank warm.

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