A biological agent for treating bottomless mud lake, preparation method and water self-purification device

Abstract

The invention discloses a biological agent for treating a bottom-mud lake, a preparation method, and a water quality self-purification device. The biological agent is composed of the following formula: 5-95 parts of Bacillus subtilis, 5-95 parts of photosynthetic bacteria, 10-80 parts of nitrifying bacteria, 10-80 parts of denitrifying bacteria, 5-75 parts of Thiobacillus, 5-75 parts of anaerobic ammonia-oxidizing bacteria, 3-70 parts of phosphate-solubilizing bacteria, 3-70 parts of sulfate-reducing bacteria, 10-70 parts of lactic acid bacteria, and 10-60 parts of yeast. The agent addition part contains the biological agent for treating a bottom-mud lake. The invention uses the water quality self-purification device and the addition of the biological agent to utilize the metabolic action of microorganisms to remove pollutants such as organic matter in the water body, degrade indicators such as COD, BOD, ammonia nitrogen, and total phosphorus, and inhibit the growth of cyanobacteria, thereby achieving self-purification and ecological restoration of the water quality of the bottom-mud artificial lake, and effectively solving the problems of cyanobacterial outbreaks and black and smelly water.

Description

Technical Field

[0001] The present invention relates to the technical field of lake water treatment, and in particular to a biological bacterial agent for treating a bottomless mud lake, a preparation method thereof, and a water quality self-purification device. Background Art

[0002] "No bottom mud" refers to an artificial lake with a cement bottom. Because of the cement bottom, the bottom of the lake does not have the ability to heal itself, and it is difficult to treat. Therefore, our biological island and bacterial agents are needed for treatment. However, since the existing methods are to manually add bacterial agents directly to specific locations in the lake, the operation is relatively unchanged. Even if traditional delivery devices are used for delivery, they are not convenient for self-propelled mobile cleaning, the cleaning area is small, and it is not convenient to set the cleaning route according to the shape of the lake. It is also not convenient to quickly recover the precipitated impurities after the natural precipitation of the bacterial agents, which easily leads to bottom mud accumulation.

[0003] To this end, we propose a biological bacterial agent for the treatment of bottomless mud lakes, a preparation method and a water quality self-purification device. Summary of the Invention

[0004] The purpose of the present invention is to provide a biological bacterial agent for the treatment of bottomless lakes, a preparation method and a water self-purification device, so as to solve the problem raised in the above background technology that the bottom of the current "bottomless lake" has no complete self-healing ability and is difficult to treat.

[0005] To achieve the above-mentioned object, the present invention provides the following technical solution: a biological agent for the treatment of a bottomless mud lake, characterized in that the biological agent comprises the following raw materials in parts by weight: 5-95 parts of Bacillus subtilis, 5-95 parts of photosynthetic bacteria, 10-80 parts of nitrifying bacteria, 10-80 parts of denitrifying bacteria, 5-75 parts of Thiobacillus, 5-75 parts of anaerobic ammonia-oxidizing bacteria, 3-70 parts of phosphate-solubilizing bacteria, 3-70 parts of sulfate-reducing bacteria, 10-70 parts of lactic acid bacteria and 10-60 parts of yeast.

[0006] Preferably, the biological agent further comprises: nutrients, trace elements, stabilizers and protective agents.

[0007] Preferably, the nutrients include at least one of a carbon source, a nitrogen source, a phosphorus source, an inorganic salt and a trace element.

[0008] Preferably, the stabilizer comprises at least one of glycerin, gelatin and lactitol.

[0009] Preferably, the protective agent includes at least one of sodium alginate, glycerol and β-cyclodextrin.

[0010] A method for preparing a biological agent for treating a bottomless mud lake comprises the following steps:

[0011] S1. Activation culture: inoculate the preserved bacteria into fresh culture medium for compound activation culture;

[0012] S2. Expanded culture: The activated strain is inoculated into a larger culture container for expanded culture. By controlling the culture conditions, the rapid growth and reproduction of the strain is promoted. During the culture process, samples are taken regularly to test the concentration and activity of the strain to ensure that the growth condition of the strain meets the requirements;

[0013] S3. Homogenization: The mixed bacteria, nutrients, trace elements, stabilizers and protective agents are fully mixed by stirring or oscillating to form a homogenized bacterial agent;

[0014] S4. Packaging: The prepared microbial agent is packaged. The packaging container should be made of sterile and well-sealed materials, such as glass bottles or plastic bottles.

[0015] S5. Storage: Store the encapsulated microbial agent in a cool, dry, dark place. At the same time, check the storage condition of the microbial agent regularly.

[0016] A water quality self-purification device for treating a bottomless mud lake comprises a purification installation member, on which a moving device is mounted, wherein the moving device is used to move and adjust the purification position; a row of fish-driving control members are mounted on the purification installation member; the fish-driving control members are used to drive away fish schools;

[0017] The mobile device is equipped with a microbial agent adding unit;

[0018] The microbial agent adding part contains biological microbial agents for treating bottom-sludge lakes;

[0019] A liquid surface floating island is installed on the bacterial agent adding part; the liquid surface floating island is used to aerate the lake water;

[0020] The mobile device is equipped with a brushing auxiliary component, and the brushing auxiliary component is used to clean the floating island component on the liquid surface;

[0021] The mobile device is equipped with a double-layer suction piece, which is used to prevent damage to aquatic plants.

[0022] The purification mounting component includes: a guide mounting seat and a raised guide rail, and a row of through holes are respectively provided on both sides of the guide mounting seat; the through holes on both sides of the guide mounting seat are used to pass bolts to install the guide mounting seat on the bottom of the water; the raised guide rail is fixedly installed on the guide mounting seat, and the guide mounting seat is composed of two round steel columns; the raised guide rail is used to guide the movement of the moving device.

[0023] Preferably, the purification mounting member further comprises: a fish-repelling seat and an underwater buzzer speaker; a row of fish-repelling seats are fixedly mounted on the side of the guide mounting seat by bolts, and underwater buzzers are fixedly mounted on a row of guide mounting seats respectively; the underwater buzzer speakers are used to drive away fish.

[0024] Preferably, the mobile device includes: a mobile bracket, a mobile extrusion rod, a waterproof driving motor, a driving sheave, a driven wheel frame, a spring shaft and a fitting matching sheave, the top of the mobile bracket is a circular structure; the mobile bracket is located outside the raised guide rail; a mobile extrusion rod is fixedly installed at the bottom of the mobile bracket, and the bottom of the mobile extrusion rod is an arc-shaped structure; two waterproof driving motors are fixedly installed on the mobile bracket; the output shafts of the two waterproof driving motors are respectively fixedly installed with driving sheaves, and the ends of the two driving sheaves are respectively rotatably installed on the mobile bracket; the two driving sheaves are attached to the raised guide rail; the tops of the two driving sheaves are respectively provided with a circle of meshing teeth; two driven wheel frames are attached to the inner side of the mobile bracket; two spring shafts are respectively fixedly installed on the two driven wheel frames, and the four spring shafts are respectively slidably inserted into the mobile bracket; the four spring shafts are respectively sleeved with springs; two fitting matching sheaves are respectively rotatably installed on the two driven wheel frames, and the two fitting matching sheaves are respectively elastically attached to the raised guide rail; the driving sheaves and the fitting matching sheaves are used to roll on the raised guide rail.

[0025] Preferably, the fish-driving control component includes: a control mounting tube, a pressing head and a delayed piezoelectric switch, the control mounting tube is fixedly sleeved on the fish-driving seat; the control mounting tube is aligned with the movable extrusion rod; the pressing head is slidably inserted on the control mounting tube, and the top of the pressing head is a hemispherical structure; a spring is provided at the bottom of the pressing head, and the spring at the bottom of the pressing head is sleeved inside the control mounting tube; the movable extrusion rod is used to move and extrude the pressing head; a delayed piezoelectric switch is fixedly installed inside the control mounting tube, and the delayed piezoelectric switch is located at the bottom of the pressing head; the delayed piezoelectric switch is electrically fused and connected to an underwater buzzer speaker.

[0026] Preferably, the bacterial agent adding part includes: a lifting mounting column, a lifting spray ring and a spacing adaptation spring, the lifting mounting column is fixedly mounted on the movable bracket; the upper section of the lifting mounting column is a hexagonal column structure; a row of lifting spray rings is slidably sleeved on the lifting mounting column; a circle of nozzles are respectively provided on the outer side of a row of the lifting spray rings; a row of lifting spray rings are connected by a hose; spacing adaptation springs are provided between a row of the lifting spray rings, and a row of spacing adaptation springs are respectively sleeved on the lifting mounting column; a row of spacing adaptation springs have the same elastic force; a row of spacing adaptation springs is used to separate a row of lifting spray rings.

[0027] Preferably, the liquid surface floating island component includes: a floating island box, an isolation mesh, a counterweight lead ring, a water filter net, a bacteria agent box and a suction pump, and a foam float is provided at the bottom of the floating island box; the floating island box is slidably plugged into the hexagonal column of the upper section of the lifting installation column; the bottom of the floating island box is fixedly installed with an isolation mesh; a row of counterweight lead rings is fixedly installed on the isolation mesh; the counterweight lead ring at the bottom is respectively connected to the side of the movable extrusion rod and the movable bracket through two steel wires; the floating island box is used to float on the liquid surface; the counterweight lead ring is used to pull down the isolation mesh; the floating island box is fixedly installed on the It is equipped with a water filter net; the water filter net is used to isolate impurities, and the bacterial agent box is fixedly installed on the floating island box; the top of the bacterial agent box is provided with an opening, and the bacterial agent box is used to store the biological bacterial agent; a water pump is fixedly installed at the bottom of the bacterial agent box, and the water pump passes through the floating island box; the water outlet pipe of the water pump is connected to the lifting spray ring above; an aeration pump is fixedly installed on the top of the floating island box, and the air jet pipe of the aeration pump passes through the floating island box, and the air jet pipe of the aeration pump is located inside the isolation mesh; the suction pump is fixedly installed on the floating island box; the suction pump is used to suck out precipitated impurities.

[0028] Preferably, the brushing auxiliary part includes: a brushing rotating ring and a cleaning brush, the brushing rotating ring is rotatably connected to the movable bracket; a gear is provided on the inner side of the brushing rotating ring, and the gear on the inner side of the brushing rotating ring is engaged with the meshing teeth on the top of the two driving groove wheels; a cleaning brush is fixedly installed on the side of the brushing rotating ring, and a row of elastic soft bristles is provided on the cleaning brush; the row of elastic soft bristles on the cleaning brush is used to brush the isolation mesh.

[0029] Preferably, the double-layer suction part includes: a double-layer suction main pipe, an inner pipe and an anti-adsorption sleeve, the double-layer suction main pipe is fixedly installed on the mobile bracket through a bracket; a row of inner pipes is fixedly installed on the double-layer suction main pipe, and absorption holes are provided on the outside of the inner pipe; the inner pipe is a rubber hose; the double-layer suction main pipe is connected to the water inlet of the suction pump through a hose, and a row of anti-adsorption sleeves is fixedly installed on the double-layer suction main pipe, and a row of anti-adsorption sleeves are respectively located outside a row of inner pipes; the anti-adsorption sleeves are rubber hoses; the anti-adsorption sleeves are used to reduce damage to aquatic plants.

[0030] Principles of sewage treatment

[0031] Biological islands replace bottom mud to carry microorganisms: biological fillers that allow microorganisms to attach and grow, such as ecological ceramsite, activated carbon, etc., are selected to provide a living environment for probiotics.

[0032] Strong reproductive capacity: The microbial population in the microbial agent has a very strong reproductive capacity, which allows it to multiply rapidly and dominate in sewage. This rapid reproduction allows the microorganisms to form a large biological colony in a relatively short period of time, thus effectively treating pollutants in sewage.

[0033] Fast metabolism: Microorganisms have a fast metabolism and can produce a variety of degradative enzymes. Under the action of microorganisms, these degradative enzymes can efficiently degrade organic matter in sewage and convert it into harmless substances such as carbon dioxide and water.

[0034] Synergistic Effects of Multiple Microorganisms: Microbial agents contain a diverse array of microorganisms capable of degrading different pollutants. These microorganisms collaborate to gradually break down complex organic matter in wastewater into simpler inorganic compounds through diverse metabolic pathways. This synergistic effect enables microbial agents to achieve greater efficiency and stability in treating complex wastewater.

[0035] The transformation capabilities of specialized microorganisms: Some specialized microbial strains are able to transform or degrade a variety of toxic substances, such as sulfur- and ammonia-containing gases, formaldehyde, benzene, and other toxic substances. Through adsorption, absorption, and metabolism, these microorganisms transform these harmful substances into harmless and odorless substances, thereby purifying water quality and improving environmental sanitation.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] The present invention uses a water self-purification device and the addition of biological agents to utilize the metabolic action of microorganisms to remove pollutants such as organic matter in the water body, degrade indicators such as COD, BOD, ammonia nitrogen, and total phosphorus, inhibit the growth of cyanobacteria, and achieve self-purification and ecological restoration of the water quality of the bottom mud artificial lake, effectively solving the problems of cyanobacteria outbreaks and black and smelly water.

[0038] The mobile device is combined with the purification installation parts, which can be moved on the bottom of the lake. The raised guide rail is used for guidance, and the raised guide rail can be set according to the shape of the lake, which can effectively increase the comprehensiveness of water treatment and better target the purification of non-flowing water. The elastically installed fitting groove wheel can realize elastic fitting and extrusion of the raised guide rail, which can be applied to the turning points of the raised guide rail, is more practical, can continuously move on the bottom of the lake, and perform steering guidance work.

[0039] The liquid surface floating island is used to aerate the water area. At the same time, the lifting spray ring can be used to spray biological bacteria or flocculants in the water area for purification. The isolation mesh used can reduce the diffusion of aeration and flocculation impurities and play an isolation role. The floating floating island box can be used to press and lift the spray ring according to the water level. A row of spacing adaptation springs is used to space the spray rings, which automatically adapt according to the water level to ensure the uniformity of the application of bacteria and drugs.

[0040] The double-layer suction piece can automatically absorb and clean impurities on the lake bottom, improve the cleaning effect of lakes without bottom mud, and prevent silt accumulation from affecting the lake ecology. The inner tube is combined with the anti-adsorption sleeve and rubber structure, which is not easy to damage aquatic plants. The double-layer structure also prevents aquatic plants from clogging the anti-adsorption sleeve and inner tube, and is not easily squeezed and bent, thereby improving the stability of impurity absorption. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 Schematic diagram of the placement of a floating island in a bottomless mud lake in Example 1 of the present invention

[0042] Figure 2 This is a schematic diagram of the overall structure of a water quality self-purification device for treating a bottomless mud lake according to the present invention;

[0043] Figure 3 This is a schematic diagram of the installation position of the mobile device of the present invention;

[0044] Figure 4 This is a schematic diagram of the bottom structure of the mobile device of the present invention;

[0045] Figure 5 For the present invention Figure 3 A magnified view of the structure of the middle A area;

[0046] Figure 6 This is a cross-sectional view of the purification installation structure of the present invention;

[0047] Figure 7 It is an enlarged schematic diagram of the structure of the mobile device of the present invention;

[0048] Figure 8 This is a structural diagram of the fish driving control component of the present invention;

[0049] Figure 9 This is a schematic diagram of the structure of the bacterial agent adding part of the present invention;

[0050] Figure 10 This is a cross-sectional view of the structure of the brushing auxiliary component of the present invention;

[0051] Figure 11 This is a cross-sectional view of the liquid surface floating island structure of the present invention;

[0052] Figure 12 This is a schematic diagram of the installation position of the brushing auxiliary component of the present invention;

[0053] Figure 13 For the present invention Figure 12 A magnified view of the structure of the middle G region.

[0054] Figure: 1. Purification mounting part; 101. Guide mounting seat; 1011. Raised guide rail; 102. Fish repelling seat; 103. Underwater buzzer speaker; 2. Moving device; 201. Moving bracket; 202. Moving extrusion rod; 203. Waterproof driving motor; 204. Driving sheave; 205. Driven sheave frame; 206. Spring shaft; 207. Fitting matching sheave; 3. Fish repelling control part; 301. Control mounting cylinder; 302. Down pressure head; 303. Delay piezoelectric switch; 4. Bacterial agent addition Part; 401, lifting installation column; 402, lifting spray ring; 403, spacing adaptation spring; 5, liquid surface floating island component; 501, floating island box; 502, isolation mesh; 5021, counterweight lead ring; 503, water filter net; 504, bacterial agent box; 505, water pump; 506, aeration pump; 507, suction pump; 6, brushing auxiliary component; 601, brushing rotating ring; 602, cleaning brush; 7, double-layer suction component; 701, double-layer suction main pipe; 702, inner pipe; 703, anti-adsorption sleeve. DETAILED DESCRIPTION

[0055] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0056] Example 1: A biological agent for treating a bottomless lake, the biological agent being composed of the following formula: 5-95 parts of Bacillus subtilis, 5-95 parts of photosynthetic bacteria, 10-80 parts of nitrifying bacteria, 10-80 parts of denitrifying bacteria, 5-75 parts of Thiobacillus, 5-75 parts of anaerobic ammonia-oxidizing bacteria, 3-70 parts of phosphate-solubilizing bacteria, 3-70 parts of sulfate-reducing bacteria, 10-70 parts of lactic acid bacteria, and 10-60 parts of yeast.

[0057] Adding auxiliary ingredients: Adding appropriate amounts of nutrients, trace elements, stabilizers and protective agent auxiliary ingredients to the biological agent. These ingredients can improve the activity and stability of the agent, extend the shelf life of the agent, and enhance the agent's ability to degrade pollutants. The added nutrients include but are not limited to: carbon source, nitrogen source, phosphorus source, inorganic salts, trace elements. The added stabilizer includes at least one of glycerol, gelatin and lactitol. The added protective agent includes at least one of sodium alginate, glycerol and β-cyclodextrin.

[0058] Equipment used: sterile operating table, constant temperature incubator, shaker, centrifuge, spectrophotometer and other experimental equipment to ensure sterile operation and precise control of the experimental process.

[0059] The role of bacteria:

[0060] (1) Bacillus subtilis

[0061] Function: Bacillus subtilis plays a vital role in wastewater treatment. As an aerobic bacterium, it effectively decomposes organic matter, reducing the chemical oxygen demand (COD) and biochemical oxygen demand (BOD) in wastewater. It uses enzymes to break down complex organic matter in wastewater, improving its biodegradability. Furthermore, Bacillus subtilis inhibits the growth of harmful bacteria through competition and the production of antimicrobial substances, thereby improving water quality.

[0062] (2) Photosynthetic bacteria

[0063] Function: Photosynthetic bacteria are a type of bacteria that can photosynthesize, using light energy, carbon dioxide, and small organic molecules to grow and reproduce. In wastewater treatment, photosynthetic bacteria can degrade pollutants such as organic matter, ammonia nitrogen, and nitrite, while also inhibiting the growth of harmful algae and improving water clarity and self-purification capabilities.

[0064] (3) Nitrifying bacteria

[0065] Function: Nitrifying bacteria are primarily responsible for converting ammonia nitrogen in wastewater into nitrite and then nitrate, a key step in the nitrogen cycle in wastewater treatment. Through nitrification, ammonia nitrogen in wastewater is removed, thereby reducing the toxicity of the water to aquatic life.

[0066] (4) Denitrifying bacteria

[0067] Function: Denitrifying bacteria are responsible for reducing the nitrates produced by nitrifying bacteria into nitrogen gas, which is then released into the air, thereby completely removing nitrogen. This process is important for reducing eutrophication and preventing black and smelly water.

[0068] (5) Thiobacillus

[0069] Function: Thiobacillus is a type of bacteria that oxidizes sulfide and produces sulfuric acid. It plays a crucial role in treating sulfide-containing wastewater, removing sulfide from the wastewater through oxidation, thereby reducing its toxicity. Furthermore, Thiobacillus can synergize with other microorganisms to promote the degradation of other pollutants in the wastewater.

[0070] (6) Anaerobic ammonium oxidizing bacteria:

[0071] Function: Anaerobic ammonium oxidizing bacteria (ANAMMOX) are bacteria that can directly convert ammonia nitrogen and nitrite into nitrogen gas under anaerobic conditions. This process does not require oxygen or an organic carbon source, making it highly energy-efficient and environmentally friendly. In wastewater treatment, ANAMMOX can be used as an effective denitrification method to reduce nitrogen emissions.

[0072] (7) Phosphate-solubilizing bacteria:

[0073] Function: Phosphate-solubilizing bacteria are a type of bacteria that can decompose organic and inorganic phosphorus compounds, releasing soluble phosphorus. In wastewater treatment, phosphate-solubilizing bacteria can help remove phosphorus pollution by breaking down phosphorus sources in wastewater, increasing phosphorus availability in the water. Furthermore, phosphate-solubilizing bacteria can form a symbiotic relationship with plant roots, promoting their absorption and utilization of phosphorus.

[0074] (8) Sulfate-reducing bacteria:

[0075] Function: Sulfate-reducing bacteria (SRBs) are a type of bacteria that can reduce sulfate to sulfide under anaerobic conditions. In wastewater treatment, they remove sulfate from water through this process, thereby reducing water acidity and heavy metal toxicity. Furthermore, SRBs can synergize with denitrifying bacteria to enhance wastewater denitrification.

[0076] (9) Lactic acid bacteria (LAB):

[0077] Function: Although lactic acid bacteria are not a mainstream bacterial species in sewage treatment, they can play a certain role under certain conditions. For example, in low-temperature or low-pH environments, lactic acid bacteria can secrete organic acids such as lactic acid to lower the pH value of sewage, thereby inhibiting the growth of harmful microorganisms and promoting the degradation of organic matter.

[0078] (10) Yeasts:

[0079] Function: Yeasts are primarily used as bioflocculants or biosorbents in wastewater treatment. They can absorb and remove pollutants such as suspended solids and heavy metal ions from wastewater, improving wastewater purification. Yeasts also secrete bioactive substances, such as enzymes and antibiotics, which influence the microbial communities in wastewater.

[0080] These strains work synergistically to promote the decomposition of organic matter, the removal of nitrogen and phosphorus, and the inhibition of cyanobacteria. The culture medium can, to a certain extent, act as a stabilizer and protective agent to ensure the activity and stability of the bacterial agent during storage and use.

[0081] A method for preparing a biological agent for treating a bottomless mud lake comprises the following steps:

[0082] S1. Activation culture: Inoculate the preserved bacterial strains onto fresh culture medium for composite activation culture. Under suitable temperature and light conditions, observe the growth of the composite bacterial strains to ensure that the composite bacterial strains have recovered their activity and are in a good growth state.

[0083] S2. Expanded culture: The activated strain is inoculated into a larger culture container for expanded culture. By controlling the culture conditions (such as temperature, pH value, dissolved oxygen, etc.), the rapid growth and reproduction of the strain is promoted. During the culture process, samples are taken regularly to test the concentration and activity of the strain to ensure that the growth of the strain meets the requirements;

[0084] S3. Homogenization: The mixed bacteria and auxiliary ingredients are thoroughly mixed by stirring or oscillating to form a homogenized inoculum. Homogenization ensures that the components in the inoculum are evenly distributed, thereby improving the effectiveness of the inoculum.

[0085] S4. Packaging: The prepared microbial agent is packaged. The packaging container should be made of sterile, well-sealed materials, such as glass bottles or plastic bottles. During the packaging process, care should be taken to avoid contamination and leakage to ensure the purity and activity of the microbial agent.

[0086] S5. Storage: Store the encapsulated microbial agent in a cool, dry, dark place to avoid direct sunlight and high temperature environment which may have adverse effects on the microbial agent. At the same time, regularly check the storage condition of the microbial agent to ensure that the microbial agent maintains good activity and stability within the validity period.

[0087] refer to Figure 1 In this embodiment, a method of adding a biological agent for treating a lake without bottom mud can be adopted, which is manual addition. The method of using the biological agent for treating a lake without bottom mud is as follows:

[0088] Dosage calculation

[0089] The dosage of bacterial agent should be reasonably calculated according to the scale of the treatment system, water quality and treatment objectives. Generally speaking, the dosage of bacterial agent for domestic sewage is about 1%-30% (i.e. 10L-300L per cubic meter), and the dosage of bacterial agent for industrial sewage is 10%-45% per cubic meter (i.e. 100L-450L per cubic meter).

[0090] Dosing point and time

[0091] Select appropriate locations in the artificial lake to set up dosing points (such as the center of the regional lake surface, around the floating island or at the intersection of water flows), and determine the dosing time and amount based on water quality monitoring results and treatment needs.

[0092] Dosing method

[0093] According to the water quality monitoring results, biological agents are added to the lake regularly. During the normal operation of the system, in order to maintain the activity and quantity of the bacteria, a batch addition method can be adopted, that is, the bacteria agent is divided into several batches and gradually added to the system over a period of time. Batch addition can reduce the impact load of the bacteria agent in the system, allow the bacteria to gradually adapt to the new environment, and help improve the treatment effect.

[0094] Direct dosing method: When re-dosing the bacterial agent at system startup or after overhaul, a one-time dosing method can be adopted, that is, the calculated total amount of bacterial agent is added to the system at one time, and the bacterial agent is directly added to a specific location of the sewage treatment system, such as the water inlet of the anaerobic tank, aerobic tank or bioreactor. This method is simple to operate, but it should be noted that the bacterial agent may lose some activity due to water impact during the dosing process.

[0095] Dilution dosing method: Mix and dilute the bacterial agent with an appropriate amount of clean water or sewage, and then add it to the treatment system. The dilution dosing method can reduce the loss of the bacterial agent during the dosing process and help to evenly distribute the bacterial agent in the system.

[0096] Activation dosing method: For some bacterial agents that require specific environmental conditions, activation treatment can be performed first. This involves mixing the bacterial agent, a nutrient source (such as glucose, urea, potassium dihydrogen phosphate, etc.), and water in a certain proportion and then activating the agent under appropriate temperature and conditions. The activated bacterial agent is more active and can better adapt to the environment of the treatment system after dosing, thereby improving treatment efficiency.

[0097] Continuous dosing: For certain treatment systems that require maintaining a high bacterial concentration, a continuous dosing method can be used. This involves continuously dosing the bacterial agent into the system at a constant flow rate through a specialized dosing device. This method ensures a continuous supply of bacterial agents and stable activity in the system, but requires careful control of the dosing amount and stable operation of the dosing device.

[0098] Maintenance Management

[0099] 1. Regular inspection and monitoring

[0100] During the process of adding bacterial agents, water quality indicators and treatment effects should be monitored regularly, and the dosage and environmental conditions should be adjusted in time according to the monitoring results to ensure stable operation and efficient treatment of the treatment system.

[0101] Monitor changes in water quality indicators (such as COD, BOD, ammonia nitrogen, total phosphorus, etc.), evaluate treatment effects, and adjust treatment plans in a timely manner.

[0102] Regularly inspect and maintain the biological island, aeration equipment and bacterial agent addition points to ensure their normal operation and effective treatment effect.

[0103] 2. Cleaning and repair

[0104] Regularly clean plant debris and impurities in biological fillers on the floating island to keep the floating island clean and promote plant growth.

[0105] 3. Supplementation and adjustment of microbial agents

[0106] According to the water quality monitoring results and treatment effects, the type and dosage of bacterial agents should be supplemented or replaced in a timely manner.

[0107] Adjust aeration intensity and operation time to adapt to water quality changes and treatment needs.

[0108] Advantages of microorganisms

[0109] 1. Strong environmental adaptability: The microorganisms in microbial agents are highly adaptable to the environment and are prone to mutation when affected by environmental factors. They can form a rapid and effective exchange of substances with the surrounding environment. This allows microbial agents to function under different water quality conditions, including changes in environmental factors such as temperature, pH, and dissolved oxygen.

[0110] 2. Low temperature resistance and impact resistance: Some special microbial agents are also resistant to low temperatures and impacts. Even in low temperatures or when water quality fluctuates greatly, these microorganisms can still maintain high activity and degradation capabilities, ensuring the effectiveness of sewage treatment.

[0111] 3. Aerobic and anaerobic treatment: In practical applications, microbial agents can treat wastewater in both aerobic and anaerobic ways. Under aerobic conditions, microorganisms oxidize and decompose organic matter into carbon dioxide and water through catabolism and anabolism. Under anaerobic conditions, microorganisms can break down large organic molecules into soluble substances, which are then further degraded by specific anaerobic bacteria into low-molecular organic acids and alcohols, ultimately producing gases such as methane.

[0112] 4. In-situ remediation: Microbial agents are used to treat water bodies without the need to extract the water. Once added, the agents rapidly multiply and degrade pollutants, quickly restoring the water's self-purification capacity. This in-situ remediation approach not only saves time and costs but also avoids potential secondary contamination during water transportation and handling.

[0113] Principles of sewage treatment

[0114] Biological islands replace bottom mud to carry microorganisms: biological fillers that allow microorganisms to attach and grow, such as ecological ceramsite, activated carbon, etc., are selected to provide a living environment for probiotics.

[0115] Strong reproductive capacity: The microbial population in the microbial agent has a very strong reproductive capacity, which allows it to multiply rapidly and dominate in sewage. This rapid reproduction allows the microorganisms to form a large biological colony in a relatively short period of time, thus effectively treating pollutants in sewage.

[0116] Fast metabolism: Microorganisms have a fast metabolism and can produce a variety of degradative enzymes. Under the action of microorganisms, these degradative enzymes can efficiently degrade organic matter in sewage and convert it into harmless substances such as carbon dioxide and water.

[0117] Synergistic Effects of Multiple Microorganisms: Microbial agents contain a diverse array of microorganisms capable of degrading different pollutants. These microorganisms collaborate to gradually break down complex organic matter in wastewater into simpler inorganic compounds through diverse metabolic pathways. This synergistic effect enables microbial agents to achieve greater efficiency and stability in treating complex wastewater.

[0118] The transformation capabilities of specialized microorganisms: Some specialized microbial strains are able to transform or degrade a variety of toxic substances, such as sulfur- and ammonia-containing gases, formaldehyde, benzene, and other toxic substances. Through adsorption, absorption, and metabolism, these microorganisms transform these harmful substances into harmless and odorless substances, thereby purifying water quality and improving environmental sanitation.

[0119] The test data of a hotel in Beijing is as follows

[0120]

[0121] Example 2:

[0122] See also Figure 2 - Figure 13 As shown:

[0123] The present invention provides a technical solution: a water quality self-purification device for the treatment of bottomless lakes, comprising a purification installation part 1, a moving device 2 installed on the purification installation part 1, and the moving device 2 is used to move and adjust the purification position; a row of fish-driving control parts 3 are installed on the purification installation part 1; the fish-driving control parts 3 are used to drive away fish schools; a microbial agent adding part 4 is installed on the moving device 2; the microbial agent adding part 4 is used to spray biological microbial agents in the lake water, and the microbial agent adding part contains biological microbial agents for the treatment of bottomless lakes; a liquid surface floating island part 5 is installed on the microbial agent adding part 4; the liquid surface floating island part 5 is used to aerate the lake water; a brush is installed on the mobile device 2 The wiping auxiliary part 6 is used to clean the liquid surface floating island part 5; a double-layer suction part 7 is installed on the mobile device 2; the double-layer suction part 7 is used to prevent damage to aquatic plants; the purification installation part 1 includes: a guide mounting seat 101 and a raised guide rail 1011, and a row of through holes are respectively provided on both sides of the guide mounting seat 101; the through holes on both sides of the guide mounting seat 101 are used to pass bolts to install the guide mounting seat 101 on the bottom of the water; a raised guide rail 1011 is fixedly installed on the guide mounting seat 101, and the guide mounting seat 101 consists of two round steel columns; the raised guide rail 1011 is used to guide the movement of the mobile device 2.

[0124] Among them, the purification installation part 1 also includes: a fish-driving seat 102 and an underwater buzzer speaker 103, a row of fish-driving seats 102 are fixedly installed on the side of the guide installation seat 101 by bolts, and underwater buzzer speakers 103 are fixedly installed on a row of guide installation seats 101; the underwater buzzer speakers 103 are used to drive away fish; the moving device 2 includes: a moving bracket 201, a moving extrusion rod 202, a waterproof driving motor 203, a driving groove wheel 204, a driven wheel frame 205, a spring shaft 206 and a fitting matching groove wheel 207, and the driving groove wheel 204 and the fitting matching groove wheel 207 are respectively provided with grooves for adapting to the raised guide rail 1011; the top of the moving bracket 201 is a circular structure; the moving bracket 201 is located outside the raised guide rail 1011; a moving extrusion rod 202 is fixedly installed on the bottom of the moving bracket 201, and the bottom of the moving extrusion rod 202 is an arc structure; Two waterproof driving motors 203 are equipped; driving sheaves 204 are fixedly mounted on the output shafts of the two waterproof driving motors 203, and the ends of the two driving sheaves 204 are rotatably mounted on the mobile bracket 201; the two driving sheaves 204 are attached to the raised guide rail 1011; the tops of the two driving sheaves 204 are respectively provided with a circle of meshing teeth; two driven wheel frames 205 are attached to the inner side of the mobile bracket 201; two spring shafts 206 are respectively fixedly mounted on the two driven wheel frames 205, and the four spring shafts 206 are respectively slidably inserted into the mobile bracket 201; springs are respectively sleeved on the four spring shafts 206; two matching sheaves 207 are respectively rotatably mounted on the two driven wheel frames 205, and the two matching sheaves 207 are respectively elastically attached to the raised guide rail 1011; the driving sheaves 204 and the matching sheaves 207 are used to roll on the raised guide rail 1011;The mobile device 2 is used in conjunction with the purification installation part 1, which can be moved on the bottom of the lake. The raised guide rail 1011 is used to guide the raised guide rail 1011, and the raised guide rail 1011 can be set according to the shape of the lake, which can effectively increase the comprehensiveness of water treatment and better target the purification of non-flowing water. The driving groove wheel 204 is used in conjunction with the fitting groove wheel 207, which can be clamped on the raised guide rail 1011 for movement. The elastically installed fitting groove wheel 207 can achieve elastic fitting and extrusion of the raised guide rail 1011, which can be suitable for the bending part of the raised guide rail 1011, which is more practical, can continue to move on the bottom of the lake, and perform steering guidance work. The guide mounting seat 101 is placed on the bottom of the lake without bottom mud and installed with bolts. According to the actual lake area and shape, the guide mounting base 101 and the raised guide rail 1011 are manufactured. The raised guide rail 1011 can be curved in an "S" shape to adapt to the lake's shape. The waterproof drive motor 203 drives the drive sheave 204 to rotate, driving the mobile bracket 201 to move on the lake bottom. Under the strong pressure of the spring on the spring shaft 206, the matching sheave 207 can elastically roll and fit at any position on the raised guide rail 1011, ensuring the normal movement and operation of the mobile bracket 201. The cylindrical structure of the raised guide rail 1011 also ensures that the drive sheave 204 and the matching sheave 207 can use their own grooves to fit and limit, preventing them from falling off.

[0125] The fish-driving control component 3 includes: a control installation tube 301, a pressure head 302 and a time-delay piezoelectric switch 303. The GE-320A underwater buzzer speaker 103 can be used to disperse the fish school. The time-delay piezoelectric switch 303 can be used with a micro switch in conjunction with a LADT2 delay module. The control installation tube 301 is fixedly sleeved on the fish-driving seat 102; the control installation tube 301 is aligned with the movable extrusion rod 202; the pressure head 302 is slidably inserted on the control installation tube 301, and the top of the pressure head 302 is a hemispherical structure; A spring is provided at the bottom of the pressure head 302, and the spring provided at the bottom of the lower pressure head 302 is sleeved inside the control installation tube 301; the mobile extrusion rod 202 is used to move and extrude the lower pressure head 302; a delayed piezoelectric switch 303 is fixedly installed inside the control installation tube 301, and the delayed piezoelectric switch 303 is located at the bottom of the lower pressure head 302; the delayed piezoelectric switch 303 is electrically fused to the underwater buzzer speaker 103; the fish driving control part 3 is used to use the mobile bracket 201 to move and apply medicine, thereby driving the mobile extrusion rod 202 to automatically control the fish driving. The work of the fish school improves the safety of the actual lake water purification treatment, and can effectively prevent the problem that the medicine is not completely diluted and mixed when the lifting spray ring 402 just sprays out the medicine, the concentration is too high, and it is easy to cause irreversible damage to the fish. This structure does not need to drive away the fish school in the entire lake area, and can be automatically controlled on the moving route of the raised guide rail 1011. At the same time, this structure uses the delayed piezoelectric switch 303, which has a delay function and pre-sets the time to ensure that the medicine is fully mixed and diluted, and automatically turns off the underwater buzzer after the water quality is purified. Device 103 ensures a normal growth environment for fish, can ensure the range of fish activities, and can avoid high concentrations of drugs damaging fish. When the movable bracket 201 moves, it drives the movable squeezing rod 202 to squeeze the pressing head 302. After the hemispherical top of the pressing head 302 is squeezed by the arc shape of the bottom of the movable squeezing rod 202, the pressing head 302 moves downward to press the delayed piezoelectric switch 303. At this time, the delayed piezoelectric switch 303 can be energized to control the underwater buzzer speaker 103 to play noise to drive away the fish. At this time, the fish will not approach the drug delivery area.

[0126] The microbial agent adding part 4 includes: a lifting installation column 401, a lifting spray ring 402 and a spacing adaptation spring 403. The lifting installation column 401 is fixedly installed on the mobile bracket 201; the upper part of the lifting installation column 401 is a hexagonal column structure; a row of lifting spray rings 402 are slidably sleeved on the lifting installation column 401; a row of lifting spray rings 402 are respectively provided with a circle of nozzles on the outside; a row of lifting spray rings 402 are connected by a hose; a row of lifting spray rings 402 are provided with a spacing adaptation spring 403 , and a row of spacing adaptation springs 403 are respectively sleeved on the lifting installation column 401; a row of spacing adaptation springs 403 have the same elastic force; a row of spacing adaptation springs 403 are used to separate a row of lifting spray rings 402; the liquid surface floating island part 5 includes: a floating island box 501, an isolation mesh 502, a counterweight lead ring 5021 and a water filter net 503, the counterweight lead ring 5021 at the bottom is connected to the side of the mobile extrusion rod 202 and the mobile bracket 201 by two steel wires; a foam float is provided at the bottom of the floating island box 501, refer to Figure 1, the floating island box 501 can be installed on the floating island in the center, and the other outer floating islands can be removed; the floating island box 501 is slidably plugged into the hexagonal column on the upper section of the lifting installation column 401; an isolation mesh 502 is fixedly installed at the bottom of the floating island box 501; a row of counterweight lead rings 5021 are fixedly installed on the isolation mesh 502; the floating island box 501 is used to float on the liquid surface; the counterweight lead rings 5021 are used to pull down the isolation mesh 502; a water filter net 503 is fixedly installed on the floating island box 501; the water filter net 503 is used to isolate impurities; the floating island on the liquid surface Component 5 also includes: a bacterial agent box 504, a water pump 505, an aeration pump 506 and a suction pump 507. The bacterial agent box 504 is fixedly installed on the floating island box 501; the top of the bacterial agent box 504 is provided with an opening; the bottom of the bacterial agent box 504 is fixedly installed with a water pump 505, and the water pump 505 passes through the floating island box 501; the outlet pipe of the water pump 505 is connected to the upper lifting spray ring 402; the top of the floating island box 501 is fixedly installed with an aeration pump 506, and the jet pipe of the aeration pump 506 passes through the floating island box 501, and the jet pipe of the aeration pump 506 is located at the bottom of the floating island box 501. The suction pump 507 is fixedly mounted on the floating island box 501; the suction pump 507 is used to suck out the precipitated impurities. The liquid surface floating island 5 is used to aerate the water area. At the same time, the lifting spray ring 402 can be used to spray biological bacteria or flocculants in the water area for purification. The isolation mesh 502 used can reduce the diffusion of aeration and flocculated impurities and play an isolation role. The floating floating island box 501 can be used to press the lifting spray ring 402 according to the water level to ensure that a row of The lifting spray rings 402 are all located in the water, and a row of spacing adaptation springs 403 are used to space the lifting spray rings 402, which automatically adapt according to the water level to ensure the uniformity of the application of the bacterial agent. When the floating island box 501 is lifted and floated, the upper hexagonal column of the lifting installation column 401 can limit the floating island box 501 to only slide vertically, preventing the isolation mesh 502 from rotating and affecting the subsequent brushing of the brushing auxiliary part 6. The counterweight lead ring 5021 can be used to pull down the isolation mesh 502 for adaptation to improve the vertical effect of the isolation mesh 502.

[0127] It should be noted that the floating island box 501 is made of environmentally friendly, weather-resistant and buoyant materials, such as HDPE (high-density polyethylene) floating boards, to ensure that the floating island can float stably in the water and can be connected to the surrounding Figure 1 Used in conjunction with the central floating island.

[0128] The layout and number of floating islands should be rationally designed based on the size, shape, and pollution level of the artificial lake to ensure coverage and purification effectiveness. In principle, they should cover 1%-15% of the total lake area, with a minimum size of 4m*4m.

[0129] At the same time, a small biological filler (not shown in the figure) can be installed on the bottom of the floating island box 501. The biological filler is a biological filler suitable for the attachment and growth of microorganisms, such as ecological ceramsite, activated carbon, etc., to provide a living environment for the probiotics.

[0130] At the same time, plants (not shown in the figure) can also be planted on the outer panels of the floating island box 501. Plant selection: According to the local climate and water quality conditions, suitable ornamental plants such as lotus, water grass, reed, cattail, etc. can be selected, while considering the water purification ability and ecological effect of the plants.

[0131] Floating island boxes 501, biofill, and plants are assembled into a complete floating island unit according to design requirements. A bio-island consisting of floating island boxes 501 and biofill (such as ecological ceramsite and activated carbon) is placed in an artificial lake. Ornamental plants such as lotus and waterweed are planted on the island, beautifying the environment and providing a substrate for microbial growth.

[0132] Example 3, based on Example 2, the brushing auxiliary part 6 includes: a brushing rotating ring 601 and a cleaning brush 602, the brushing rotating ring 601 is rotatably sleeved on the mobile bracket 201; a gear is provided on the inner side of the brushing rotating ring 601, and the gear on the inner side of the brushing rotating ring 601 is engaged with the meshing teeth on the top of the two driving groove wheels 204; a cleaning brush 602 is fixedly installed on the side of the brushing rotating ring 601, and a row of elastic soft bristles is provided on the cleaning brush 602; the row of elastic soft bristles provided on the cleaning brush 602 is used to brush Wipe the isolation mesh 502; the double-layer suction member 7 includes: a double-layer suction main pipe 701 and an inner pipe 702, the double-layer suction main pipe 701 is fixedly mounted on the mobile bracket 201 through a bracket; a row of inner pipes 702 are fixedly mounted on the double-layer suction main pipe 701, and the outer side of the inner pipe 702 is provided with an absorption hole; the inner pipe 702 is a rubber hose; the double-layer suction main pipe 701 is connected to the water inlet of the suction pump 507 through a hose; the double-layer suction member 7 also includes: an anti-adsorption sleeve 703, fixedly mounted on the double-layer suction main pipe 701 There is a row of anti-adsorption sleeves 703, and a row of anti-adsorption sleeves 703 are respectively located outside a row of inner tubes 702; the anti-adsorption sleeves 703 are rubber hoses; the anti-adsorption sleeves 703 are used to reduce damage to aquatic plants, and adopt a brushing auxiliary part 6, which can facilitate automatic brushing of the isolation mesh 502, and assist in improving the isolation effect of the isolation mesh 502. It is automatically controlled and has a simple and reasonable structure, which can prevent the isolation mesh 502 from being blocked as a whole and causing poor water circulation. The double-layer suction part 7 can automatically target impurities on the lake bottom. It performs absorption and cleaning work, improves the cleaning effect of lakes without bottom mud, and prevents silt accumulation from affecting the lake ecology. The inner tube 702 is combined with the anti-absorption sleeve 703 and the rubber structure, which is not easy to damage aquatic plants. The double-layer structure also prevents aquatic plants from clogging the anti-absorption sleeve 703 and the inner tube 702, and is not easy to be squeezed and bent, thereby improving the stability of absorbing impurities and being more suitable for underwater environments. Part of the sediment flocculent matter and part of the silt can be absorbed and discharged into the floating island box 501 and filtered through the filter water net 503, and then continue to be discharged into the lake water for circulation.

[0133] The working principle of this embodiment is as follows: first, the guide mounting seat 101 is placed on the bottom of the lake without bottom mud, and fixed by bolts. According to the actual area and shape of the lake, the guide mounting seat 101 and the raised guide rail 1011 are produced. The raised guide rail 1011 can have an "S" shape to adapt to the shape of the lake. The driving groove wheel 204 set by the waterproof driving motor 203 rotates, which can drive the mobile bracket 201 to move on the bottom of the lake; when the mobile bracket 201 moves, it drives the mobile extrusion rod 202 to squeeze the lower pressure head 302. After the hemispherical top of the lower pressure head 302 is squeezed by the arc shape of the bottom of the mobile extrusion rod 202, the lower pressure head 302 is lowered. The pressure head 302 moves downward to press the delayed piezoelectric switch 303. At this time, the delayed piezoelectric switch 303 is energized to control the underwater buzzer speaker 103 to play the noise to drive away the fish. At this time, the fish will not approach. At this time, the water pump 505 extracts the bacteria or medicine in the bacteria box 504 for spraying. The bacteria or medicine gradually mixes in this area for purification. Flocculants can be used to assist in flocculating impurities and improve the water quality of the lake. Biological bacteria agents can improve the self-cleaning ability of the lake water. The flocculated impurities are isolated inside the isolation mesh 502 and flocculate downward and settle on the bottom of the lake. As the mobile bracket 201 continues to move, the corresponding water pump 505 is pressed again to control the corresponding water pump 505. The buzzer speaker 103 drives away the fish. As time goes by, the medicine is fully mixed with the lake water and diluted. The delayed piezoelectric switch 303 automatically cuts off the power, preventing the underwater buzzer speaker 103 from continuously making sounds and affecting the fish activities. As the mobile bracket 201 moves, the floating island box 501 floats on the water surface. The floating island box 501 presses the lifting spray ring 402, and a row of spacing adaptation springs 403 are compressed, automatically spacing a row of lifting spray rings 402. The bacterial agent box 504 is opened at the top to add biological bacteria inside. The water pump 505 sucks the biological bacteria and discharges it through the lifting spray ring 402. The aeration pump 506 can fill air into the water for aeration. The air works, and the flocculated impurities of the medicine are precipitated on the bottom of the lake; when the movable bracket 201 moves, it drives the double-layer suction main pipe 701 to move at the same time, and the inner pipe 702 and the anti-adsorption sleeve 703 are elastically fitted to the bottom of the water. The suction is sucked by the suction pump 507, and part of the precipitated flocculants and part of the silt can be absorbed and discharged into the floating island box 501, filtered through the filter water net 503, and then continued to be discharged into the lake water for circulation, so as to maintain the effect of the lake bottom sludge pollution. When the waterproof driving motor 203 drives the driving groove wheel 204 to rotate and move, the meshing teeth on the driving groove wheel 204 engage and drive the brush wiping rotating ring 601 to rotate, and control the cleaning brush 602 to rotate and brush the isolation mesh 502 to perform cleaning work.

[0134] It should be noted that, in this document, relational terms such as first and second, etc., are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not explicitly listed, or elements that are inherent to such process, method, article, or apparatus.

[0135] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A biological agent for treating bottomless mud lakes, characterized in that: The biological agent comprises the following raw materials in parts by weight: 5-95 parts of Bacillus subtilis, 5-95 parts of photosynthetic bacteria, 10-80 parts of nitrifying bacteria, 10-80 parts of denitrifying bacteria, 5-75 parts of Thiobacillus, 5-75 parts of anaerobic ammonia-oxidizing bacteria, 3-70 parts of phosphate-solubilizing bacteria, 3-70 parts of sulfate-reducing bacteria, 10-70 parts of lactic acid bacteria, and 10-60 parts of yeast; The biological agent further comprises: nutrients, trace elements, stabilizers and protective agents; the stabilizer comprises at least one of glycerol, gelatin and lactitol; the protective agent comprises at least one of sodium alginate, glycerol and β-cyclodextrin; The method for preparing the biological agent for treating a bottomless mud lake comprises the following steps: S1. Activation culture: inoculate the preserved bacteria into fresh culture medium for compound activation culture; S2. Expanded culture: The activated strain is inoculated into a larger culture container for expanded culture. By controlling the culture conditions, the rapid growth and reproduction of the strain is promoted. During the culture process, samples are taken regularly to test the concentration and activity of the strain to ensure that the growth condition of the strain meets the requirements; S3. Homogenization: The mixed bacteria, nutrients, trace elements, stabilizers and protective agents are fully mixed by stirring or oscillating to form a homogenized bacterial agent; S4. Packaging: The prepared microbial agent is packaged. The packaging container should be made of sterile and well-sealed materials, such as glass bottles or plastic bottles. S5. Storage: Store the encapsulated microbial agent in a cool, dry, dark place. At the same time, check the storage condition of the microbial agent regularly.

2. The biological agent for treating bottomless mud lake according to claim 1, characterized in that: The nutrients include at least one of a carbon source, a nitrogen source, a phosphorus source, an inorganic salt and a trace element.

3. A water quality self-purification device for treating a bottomless lake comprising the biological microbial agent for treating a bottomless lake as claimed in any one of claims 1 to 2, characterized in that: The invention comprises a purification installation part (1), wherein a moving device (2) is installed on the purification installation part (1), and the characteristics are: the moving device (2) is used to move and adjust the purification position; a row of fish driving control parts (3) are installed on the purification installation part (1); the fish driving control parts (3) are used to drive away fish schools; The mobile device (2) is provided with a microbial agent adding portion (4); the microbial agent adding portion (4) contains the biological microbial agent for treating a bottomless mud lake as claimed in claim 1; A liquid surface floating island component (5) is installed on the bacterial agent adding portion (4); the liquid surface floating island component (5) is used to aerate the lake water; The moving device (2) is equipped with a brushing auxiliary part (6), and the brushing auxiliary part (6) is used to clean the liquid surface floating island part (5); A double-layer suction piece (7) is installed on the mobile device (2); the double-layer suction piece (7) is used to prevent damage to aquatic plants; The purification mounting member (1) comprises: a guide mounting seat (101) and a raised guide rail (1011), wherein a row of through holes are respectively provided on both sides of the guide mounting seat (101); the through holes on both sides of the guide mounting seat (101) are used to pass bolts to install the guide mounting seat (101) on the bottom of the water; the raised guide rail (1011) is fixedly mounted on the guide mounting seat (101), and the guide mounting seat (101) is composed of two round steel columns; the raised guide rail (1011) is used to guide the movement of the moving device (2).

4. The water quality self-purification device for treating a bottomless mud lake according to claim 3 is characterized by: The purification installation part (1) further comprises: a fish-driving seat (102) and an underwater buzzer speaker (103); a row of fish-driving seats (102) are fixedly mounted on the side of the guide installation seat (101) by bolts; and underwater buzzers (103) are fixedly mounted on each of the row of guide installation seats (101); the underwater buzzers (103) are used to drive away fish; the moving device (2) comprises: a moving bracket (201), a moving extrusion rod (202), a waterproof driving motor (203), a driving groove wheel (204), a driven wheel frame (205), a spring shaft (206) and a fitting groove wheel (207); the top of the moving bracket (201) is a circular structure; the moving bracket (201) is located at a raised position. The outer side of the guide rail (1011); a movable extrusion rod (202) is fixedly installed at the bottom of the movable bracket (201), and the bottom of the movable extrusion rod (202) is an arc-shaped structure; two waterproof driving motors (203) are fixedly installed on the movable bracket (201); driving groove wheels (204) are respectively fixedly installed on the output shafts of the two waterproof driving motors (203), and the ends of the two driving groove wheels (204) are respectively rotatably installed on the movable bracket (201); the two driving groove wheels (204) are attached to the raised guide rail (1011); the tops of the two driving groove wheels (204) are respectively provided with a circle of meshing teeth; two driven wheel frames (205) are attached to the inner side of the movable bracket (201); the two driven wheels Two spring shafts (206) are fixedly mounted on the frame (205), and the four spring shafts (206) are respectively slidably plugged into the movable bracket (201); the four spring shafts (206) are respectively sleeved with springs; two driven wheel frames (205) are respectively rotatably mounted with two fitting groove wheels (207), and the two fitting groove wheels (207) are respectively elastically fitted on the raised guide rail (1011); the driving groove wheel (204) and the fitting groove wheel (207) are used to roll on the raised guide rail (1011); the fish driving control component (3) comprises: a control mounting cylinder (301), a lower pressure head (302) and a time-delay piezoelectric switch (303); the control mounting cylinder (301) is fixed The invention is sleeved on the fish driving seat (102); the control installation cylinder (301) is aligned with the movable extrusion rod (202); a pressing head (302) is slidably inserted on the control installation cylinder (301), and the top of the pressing head (302) is a hemispherical structure; a spring is provided at the bottom of the pressing head (302), and the spring provided at the bottom of the pressing head (302) is sleeved inside the control installation cylinder (301); the movable extrusion rod (202) is used to move and extrude the pressing head (302); a time-delay piezoelectric switch (303) is fixedly installed inside the control installation cylinder (301), and the time-delay piezoelectric switch (303) is located at the bottom of the pressing head (302); the time-delay piezoelectric switch (303) is electrically fused and connected to the underwater buzzer speaker (103).

5. The water quality self-purification device for treating a bottomless mud lake according to claim 4 is characterized in that: The microbial agent adding part (4) comprises: a lifting installation column (401), a lifting liquid spraying ring (402) and a spacing adaptation spring (403), wherein the lifting installation column (401) is fixedly mounted on the movable bracket (201); the upper section of the lifting installation column (401) is a hexagonal column structure; a row of lifting liquid spraying rings (402) are slidably sleeved on the lifting installation column (401); a circle of spray heads are respectively provided on the outer side of a row of lifting liquid spraying rings (402); a row of lifting liquid spraying rings (402) are connected by a hose; a row of lifting liquid spraying rings (402) are provided with a spacing adaptation spring (403), and a row of spacing adaptation springs ( 403) are respectively sleeved on the lifting installation column (401); a row of spacing adaptation springs (403) have the same elastic force; a row of spacing adaptation springs (403) are used to space a row of lifting spray rings (402), the liquid surface floating island member (5) includes: a floating island box (501), an isolation mesh (502), a counterweight lead ring (5021), a water filter net (503), a bacterial agent box (504) and a suction pump (507), and a foam floating block is provided at the bottom of the floating island box (501); the floating island box (501) is slidably plugged into the hexagonal column of the upper section of the lifting installation column (401); the bottom of the floating island box (501) is fixedly installed with an isolation mesh (502); a row of weighted lead rings (5021) are fixedly mounted on the isolation mesh (502); the weighted lead ring (5021) at the bottom is connected to the side of the movable extrusion rod (202) and the movable bracket (201) through two steel wires; the floating island box (501) is used to float on the liquid surface; the weighted lead ring (5021) is used to pull down the isolation mesh (502); a water filter net (503) is fixedly mounted on the floating island box (501); the water filter net (503) is used to isolate impurities, and the bacterial agent box (504) is fixedly mounted on the floating island box (501); the top of the bacterial agent box (504) is provided with an opening, and the bacterial agent box (504) is provided with an opening. The agent box (504) is used to store the biological agent; a water pump (505) is fixedly installed at the bottom of the agent box (504), and the water pump (505) passes through the floating island box (501); the water outlet pipe of the water pump (505) is connected to the upper lifting spray ring (402); an aeration pump (506) is fixedly installed on the top of the floating island box (501), and the air injection pipe of the aeration pump (506) passes through the floating island box (501), and the air injection pipe of the aeration pump (506) is located inside the isolation mesh (502); the suction pump (507) is fixedly installed on the floating island box (501); the suction pump (507) is used to suck out precipitated impurities.

6. The water quality self-purification device for treating a bottomless mud lake according to claim 5 is characterized by: The brushing auxiliary part (6) includes: a brushing rotating ring (601) and a cleaning brush (602), the brushing rotating ring (601) is rotatably sleeved on the movable bracket (201); a gear is provided on the inner side of the brushing rotating ring (601), and the gear on the inner side of the brushing rotating ring (601) is engaged with the meshing teeth on the top of the two driving groove wheels (204); a cleaning brush (602) is fixedly installed on the side of the brushing rotating ring (601), and a row of elastic soft bristles is provided on the cleaning brush (602); the row of elastic soft bristles provided on the cleaning brush (602) is used to brush the isolation mesh (502), and the double-layer suction part (7) includes: a double-layer suction main pipe (701), an inner pipe (702) and an anti- Adsorption sleeve (703), the double-layer suction main pipe (701) is fixedly mounted on the mobile bracket (201) through a bracket; a row of inner pipes (702) is fixedly mounted on the double-layer suction main pipe (701), and absorption holes are provided on the outer sides of the inner pipes (702); the inner pipes (702) are rubber hoses; the double-layer suction main pipe (701) is connected to the water inlet of the suction pump (507) through a hose; a row of anti-adsorption sleeves (703) is fixedly mounted on the double-layer suction main pipe (701), and a row of anti-adsorption sleeves (703) are respectively located outside a row of inner pipes (702); the anti-adsorption sleeves (703) are rubber hoses; the anti-adsorption sleeves (703) are used to reduce damage to aquatic plants.

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