Hemp water liquid fertilizer containing pest repellent as well as preparation method and application of hemp water liquid fertilizer

Abstract

The invention discloses a hemp water liquid fertilizer containing a pest repellent as well as a preparation method and application of the hemp water liquid fertilizer. The hemp water liquid fertilizer is prepared from the following components in parts by weight through mixed strain fermentation: 800-1000 parts of pretreated hemp water, 50-80 parts of castor bean meal, 10-20 parts of mint leaves, 5-15 parts of star anise, 3-8 parts of Chinese prickly ash, 2-10 parts of chitosan and 1-5 parts of a thickening agent. The mixed strain is prepared by mixing a mixed strain of domesticated bacillus thuringiensis, bacillus subtilis, bacillus mojavensis, lactobacillus casei and rhizopus oryzae with a photosynthetic bacterium strain. The invention also discloses an application of the hemp water liquid fertilizer in the aspect of expelling vegetable pests. According to the present invention, the hemp water can be turned into the valuable, the organic fertilizer is provided for vegetable growth, and the hemp water and the botanical insect repelling material act together to achieve the certain insect repelling effect so as to well promote the vegetable growth.

Description

Technical Field

[0001] The invention relates to the technical field of comprehensive utilization of hemp water, in particular to a hemp water liquid fertilizer containing an insect repellent, and a preparation method and application thereof. Background Art

[0002] Guangxi has rich sisal resources. After sisal is harvested, it can be obtained through scraping, combing, polishing, drying and other procedures to obtain sisal fiber. During the above-mentioned treatment process, a large amount of sisal water will be produced in the scraping stage. If the sisal water is directly discarded, it will produce a strong sour smell, causing a certain degree of pollution to the environment. In addition, sisal wastewater also contains nutrients such as sugars, proteins, saponins and alkaloids. If they can be utilized, it can also turn waste into treasure and reduce the waste of water resources.

[0003] Vegetables are an essential food in our daily life. Leafy vegetables have relatively large leaf areas and absorb nutrients faster than roots, making them easier for vegetables to absorb and utilize. If hemp water is made into liquid fertilizer, it can be used directly as foliar fertilizer, thus realizing the resource utilization of hemp water.

[0004] In addition, in the process of vegetable cultivation, in order to ensure the healthy growth of vegetables, in addition to supplementing nutrients, pest control is also a very important link. The traditional method of spraying chemical pesticides is likely to leave harmful ingredients in the vegetables, which will cause certain negative effects on the human body. After the hemp water is fermented, there will be some special smell. If it can be used to help drive away pests, it will be conducive to the greater use of hemp water, ensuring the yield and quality of vegetables, and reducing the negative impact of vegetable pesticide residues on the human body. Summary of the invention

[0005] The invention discloses a hemp water liquid fertilizer containing an insect repellent, a preparation method and an application thereof, which can turn hemp water into treasure, provide organic fertilizer for vegetable growth, and work together with plant-based insect repellent materials to exert a certain insect repellent effect and better promote vegetable growth.

[0006] To achieve the above object, the technical solution of the present invention is:

[0007] A hemp water liquid fertilizer containing an insect repellent is prepared by fermenting the following ingredients in parts by weight through mixed strains: 800-1000 parts of pretreated hemp water, 50-80 parts of castor meal, 10-20 parts of mint leaves, 5-15 parts of star anise, 3-8 parts of prickly ash, 2-10 parts of chitosan, and 1-5 parts of a thickener. The mixed strains are prepared by mixing tamed mixed strains of Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaivi, Lactobacillus casei and Rhizopus oryzae with photosynthetic bacterial strains.

[0008] Preferably, the pretreated hemp water is prepared by adding lime to the hemp water to adjust the pH to 5.5-6.5.

[0009] Preferably, the preparation method of the mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae is as follows: Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae are inoculated into a domestication medium containing hemp water for domestication, wherein the domestication medium containing hemp water is a liquid culture medium, and 1-2 g / L of mint juice and 0.3-0.8 g / L of pepper water are added, and the concentration of hemp water is set to 12-14 g / L, 24-28 g / L, 48-56 g / L, and 96-112 g / L, respectively. Each concentration gradient is passaged 2-3 times, cultured for 24-48 hours, and then inoculated to the next concentration gradient after repeated domestication until domestication is completed, and then inoculated into an expansion culture medium, and cultured step by step, and the target of each level of culture is set to reach a composite viable bacteria number of 1×10 8 ~1×10 9 cfu / mL, and after the cultivation is completed, dry powder is prepared to obtain domesticated Bacillus thuringiensis, domesticated Bacillus subtilis, domesticated Bacillus mohaiwei, domesticated Lactobacillus casei and domesticated Rhizopus oryzae respectively, which are then mixed in a weight ratio of 5-10:5-10:1-2:2-3:1-2 to obtain a mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae.

[0010] Preferably, the acclimation medium containing hemp water also contains the following ingredients: 250-300 g / L glucose, 10-15 g / L corn steep liquor, 10-15 g / L potato juice, 0.1-0.2 g / L MgSO4, and 0.3-1.0 g / L NaCl.

[0011] Preferably, the expansion medium contains the following ingredients: 250-300 g / L glucose, 10-15 g / L corn steep liquor, 10-15 g / L potato juice, 15-22 g / L CaCO3, 0.5-1.0 g / L KH2PO4, 0.1-0.2 g / L MgSO4, and 0.3-1.0 g / L NaCl.

[0012] Preferably, the weight ratio of the domesticated mixed strains of Bacillus thuringiensis, Bacillus subtilis, Bacillus mohairi, Lactobacillus casei and Rhizopus oryzae to the photosynthetic bacteria is 10-20:1.

[0013] The present invention also provides a method for preparing the above-mentioned liquid fertilizer containing insect repellent, comprising the following steps:

[0014] Add the mixed strains to the pretreated hemp water, mix and ferment for 3 to 4 days at 20 to 25°C, then add crushed mint leaves, crushed star anise and pepper, and castor meal, and continue to ferment for 7 to 12 days at 20 to 25°C. After the fermentation is completed, adjust the pH to 6.0 to 6.5, add chitosan and thickener, mix well, and obtain hemp water liquid fertilizer containing pest repellent.

[0015] The present invention also provides the use of the above-mentioned hemp water liquid fertilizer or the hemp water liquid fertilizer prepared by the above-mentioned preparation method in expelling vegetable pests.

[0016] Preferably, the vegetable pest is Plutella xylostella or Aphid.

[0017] The above-mentioned hemp water liquid fertilizer containing pest repellent has the following advantages:

[0018] (1) Castor oil contains rich nutrients such as carbohydrates, proteins, saponins and alkaloids. When combined with castor meal, it can be better converted into small molecules after fermentation by bacteria and made into liquid fertilizer to better meet the growth needs of vegetables.

[0019] (2) The fermentation of sisal water by bacteria can avoid the odor caused by the waste of sisal water, better protect the environment, and at the same time increase the added value of sisal utilization.

[0020] (3) The present invention also introduces mint leaves, star anise and peppermint as insect repellent ingredients, which are fermented together with hemp water. The odor produced can effectively repel vegetable pests, especially the diamondback moth or vegetable aphid, and can reduce the negative effects of pesticides on vegetables, soil and human body. DETAILED DESCRIPTION

[0021] The present invention is further described below in conjunction with specific embodiments, but the protection scope of the present invention is not limited to the following embodiments.

[0022] The preparation method of the mint juice adopted in this embodiment is: take fresh mint leaves, add 4 times the weight of water, squeeze the juice with a juicer, filter, and concentrate the liquid to 2 times the weight of the fresh mint leaves.

[0023] The preparation method of the pepper water used in this embodiment is: take dry pepper, add 15 times the weight of water, boil and keep boiling for 30 minutes, filter and separate the filtrate and residue, add 15 times the weight of water to the residue, boil and keep boiling for 30 minutes, filter and separate the filtrate, combine the two filtrates, and concentrate to twice the weight of the dry pepper.

[0024] Example 1

[0025] A hemp water liquid fertilizer containing an insect repellent is prepared by fermenting the following components in parts by weight through mixed strains: 900 parts of pretreated hemp water, 70 parts of castor meal, 18 parts of mint leaves, 10 parts of star anise, 5 parts of prickly ash, 8 parts of chitosan, and 2 parts of xanthan gum, wherein the mixed strain is prepared by mixing domesticated mixed strains of Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaivi, Lactobacillus casei, and Rhizopus oryzae with photosynthetic bacterial strains.

[0026] The pre-treated hemp water is prepared by adding lime to the hemp water in advance to adjust the pH to 6.0.

[0027] The preparation method of the mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae is as follows: Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae are inoculated into a domestication medium containing hemp water for domestication, respectively, the domestication medium containing hemp water is a liquid culture medium, the concentration of hemp water is set to 12g / L, 24g / L, 48g / L, 96g / L in sequence, each concentration gradient is passaged twice, cultured for 48h, and after repeated domestication, inoculated to the next concentration gradient until domestication is completed, and then inoculated into an expansion medium, and cultured step by step, and the target of each level of culture is set to reach a composite viable bacteria number of 1×10 9 cfu / mL, and after the cultivation is completed, dry powder is made to obtain domesticated Bacillus thuringiensis, domesticated Bacillus subtilis, domesticated Bacillus mohaiwei, domesticated Lactobacillus casei and domesticated Rhizopus oryzae respectively, which are then mixed in a weight ratio of 8:8:1:2:2 to obtain a mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae.

[0028] The acclimation medium containing hemp water also contains the following ingredients: 280 g / L glucose, 15 g / L corn steep liquor, 12 g / L potato juice, 0.1 g / L MgSO4, 0.5 g / L NaCl, 1.8 g / L mint juice, and 0.5 g / L pepper. The acclimation medium containing hemp water is sterilized at high temperature in advance.

[0029] The expansion medium contains the following ingredients: glucose 280 g / L, corn steep liquor 15 g / L, potato juice 12 g / L, CaCO3 20 g / L, KH2PO4 0.8 g / L, MgSO4 0.1 g / L, and NaCl 0.5 g / L.

[0030] Preferably, the weight ratio of the mixed strains of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohairi, Lactobacillus casei and Rhizopus oryzae to the photosynthetic bacteria is 16:1.

[0031] The method for preparing liquid hemp fertilizer containing pest repellent comprises the following steps:

[0032] Add the mixed strains into the pretreated hemp water, mix and ferment at 25°C for 4 days, then add crushed mint leaves, crushed star anise and pepper, and castor meal, and continue to ferment at 25°C for 10 days. After the fermentation is completed, adjust the pH to 6.5, add chitosan and xanthan gum, mix well, and obtain hemp water liquid fertilizer containing pest repellent.

[0033] Example 2

[0034] A hemp water liquid fertilizer containing an insect repellent is prepared by fermenting the following components in parts by weight through mixed strains: 800 parts of pretreated hemp water, 50 parts of castor meal, 10 parts of mint leaves, 5 parts of star anise, 4 parts of prickly ash, 4 parts of chitosan and 1 part of xanthan gum, wherein the mixed strain is prepared by mixing domesticated mixed strains of Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaivi, Lactobacillus casei and Rhizopus oryzae with photosynthetic bacterial strains.

[0035] The pre-treated hemp water is prepared by adding lime to the hemp water in advance to adjust the pH to 6.0.

[0036] The preparation method of the mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae is as follows: Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae are inoculated into a domestication medium containing hemp water for domestication, respectively, the domestication medium containing hemp water is a liquid culture medium, the concentration of hemp water is set to 12g / L, 24g / L, 48g / L, 96g / L in sequence, each concentration gradient is passaged twice, cultured for 48h, and after repeated domestication, inoculated to the next concentration gradient until domestication is completed, and then inoculated into an expansion medium, and cultured step by step, and the target of each level of culture is set to reach a composite viable bacteria number of 1×10 9 cfu / mL, and after the cultivation is completed, dry powder is made to obtain domesticated Bacillus thuringiensis, domesticated Bacillus subtilis, domesticated Bacillus mohaiwei, domesticated Lactobacillus casei and domesticated Rhizopus oryzae respectively, which are then mixed in a weight ratio of 8:8:2:3:2 to obtain a mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae.

[0037] The acclimation medium containing hemp water also contains the following ingredients: 280 g / L glucose, 15 g / L corn steep liquor, 12 g / L potato juice, 0.1 g / L MgSO4, 0.5 g / L NaCl, 1.8 g / L mint juice, and 0.5 g / L pepper. The acclimation medium containing hemp water is sterilized at high temperature in advance.

[0038] The expansion medium contains the following ingredients: glucose 280 g / L, corn steep liquor 15 g / L, potato juice 12 g / L, CaCO3 20 g / L, KH2PO4 0.8 g / L, MgSO4 0.1 g / L, and NaCl 0.5 g / L.

[0039] Preferably, the weight ratio of the mixed strains of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohairi, Lactobacillus casei and Rhizopus oryzae to the photosynthetic bacteria is 16:1.

[0040] The method for preparing liquid hemp fertilizer containing pest repellent comprises the following steps:

[0041] Add the mixed strains into the pretreated hemp water, mix and ferment at 25°C for 4 days, then add crushed mint leaves, crushed star anise and pepper, and castor meal, and continue to ferment at 25°C for 10 days. After the fermentation is completed, adjust the pH to 6.5, add chitosan and xanthan gum, mix well, and obtain hemp water liquid fertilizer containing pest repellent.

[0042] Example 3

[0043] A hemp water liquid fertilizer containing an insect repellent is prepared by fermenting the following components in parts by weight through mixed strains: 950 parts of pretreated hemp water, 80 parts of castor meal, 20 parts of mint leaves, 15 parts of star anise, 8 parts of prickly ash, 8 parts of chitosan, and 3 parts of sodium carboxymethyl cellulose, wherein the mixed strain is prepared by mixing domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaivi, Lactobacillus casei, and Rhizopus oryzae mixed strains with photosynthetic bacterial strains.

[0044] The pre-treated hemp water is prepared by adding lime to the hemp water in advance to adjust the pH to 6.5.

[0045] The preparation method of the mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae is as follows: Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae are inoculated into a domestication medium containing hemp water for domestication, respectively, the domestication medium containing hemp water is a liquid culture medium, the concentration of hemp water is set to 14g / L, 28g / L, 56g / L, 112g / L in sequence, each concentration gradient is passaged twice, cultured for 48h, and then inoculated to the next concentration gradient after repeated domestication until domestication is completed, and then inoculated into an expansion medium, and cultured step by step, and the target of each level of culture is set to reach a composite viable bacteria number of 1×10 9cfu / mL, and after the cultivation is completed, dry powder is made to obtain domesticated Bacillus thuringiensis, domesticated Bacillus subtilis, domesticated Bacillus mohaiwei, domesticated Lactobacillus casei and domesticated Rhizopus oryzae respectively, which are then mixed in a weight ratio of 10:10:1:2:2 to obtain a mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae.

[0046] The acclimation medium containing hemp water also contains the following ingredients: 300 g / L glucose, 15 g / L corn steep liquor, 10 g / L potato juice, 0.1 g / L MgSO4, 0.5 g / L NaCl, 1.8 g / L mint juice, and 0.5 g / L pepper. The acclimation medium containing hemp water is sterilized at high temperature in advance.

[0047] The expansion medium contained the following ingredients: glucose 300 g / L, corn steep liquor 15 g / L, potato juice 10 g / L, CaCO3 22 g / L, KH2PO4 1.0 g / L, MgSO4 0.2 g / L, and NaCl 1.0 g / L.

[0048] Preferably, the weight ratio of the mixed strains of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohairi, Lactobacillus casei and Rhizopus oryzae to the photosynthetic bacteria is 10:1.

[0049] The method for preparing liquid hemp fertilizer containing pest repellent comprises the following steps:

[0050] Add the mixed strains into the pretreated hemp water, mix and ferment at 25°C for 4 days, then add crushed mint leaves, crushed star anise and pepper, and castor meal, and continue to ferment at 25°C for 10 days. After the fermentation is completed, adjust the pH to 6.2, add chitosan and xanthan gum, mix well, and obtain hemp water liquid fertilizer containing pest repellent.

[0051] Comparative Example 1

[0052] A hemp water liquid fertilizer containing an insect repellent is prepared by fermenting the following components in parts by weight through mixed strains: 900 parts of pretreated hemp water, 70 parts of castor meal, 18 parts of mint leaves, 10 parts of star anise, 5 parts of peppermint, 8 parts of chitosan and 2 parts of xanthan gum, wherein the mixed strains are mixed strains of Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaivi, Lactobacillus casei and Rhizopus oryzae with photosynthetic bacterial strains.

[0053] The pre-treated hemp water is prepared by adding lime to the hemp water in advance to adjust the pH to 6.0.

[0054] Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaivi, Lactobacillus casei and Rhizopus oryzae are mixed in a weight ratio of 8:8:1:2:2, and then mixed with photosynthetic bacteria species in a weight ratio of 16:1.

[0055] The method for preparing liquid hemp fertilizer containing pest repellent comprises the following steps:

[0056] Add the mixed strains into the pretreated hemp water, mix and ferment at 25°C for 4 days, then add crushed mint leaves, crushed star anise and pepper, and castor meal, and continue to ferment at 25°C for 10 days. After the fermentation is completed, adjust the pH to 6.5, add chitosan and xanthan gum, mix well, and obtain hemp water liquid fertilizer containing pest repellent.

[0057] Comparative Example 2

[0058] Comparative Example 2 is basically the same as Comparative Example 1, but in the preparation method, the pretreated hemp water, mixed strains are mixed with mint leaves, crushed star anise and peppermint, and castor meal, and mixed and fermented at 25°C for 14 days. After the fermentation is completed, the pH is adjusted to 6.5, chitosan and xanthan gum are added, and mixed evenly to obtain hemp water liquid fertilizer containing pest repellent.

[0059] Comparative Example 3

[0060] Comparative Example 3 refers to Comparative Example 1, but the pretreated hemp water is replaced by water, and the fermentation is carried out once, mixed and fermented at 25°C for 14 days. After the fermentation is completed, the pH is adjusted to 6.5, chitosan and xanthan gum are added, and mixed evenly to obtain a pest repellent.

[0061] The following experimental verification is carried out using Chinese cabbage as an example.

[0062] Plant spring Chinese cabbage in early February 2024. Before planting, turn the soil, dig trenches and apply fertilizers. Apply 1500kg / mu of decomposed organic fertilizer and 50kg / mu of compound fertilizer. Plant cabbage seedlings with a row spacing of 50cm×40cm. Use the hemp water liquid fertilizer / pest repellent containing insect repellent of the above-mentioned Examples 1-3 and Comparative Examples 1-3 for topdressing. Apply topdressing once after planting and survival, in the rosette stage and the early stage of heart-wrapping. Among them, apply 200kg / mu of hemp water liquid fertilizer / pest repellent containing insect repellent after planting and survival, and apply 280kg / mu of hemp water liquid fertilizer / pest repellent containing insect repellent in the rosette stage and the early stage of heart-wrapping. In addition, when using the insect repellent of Comparative Example 3, high-nitrogen compound fertilizer is also applied in combination. Apply 25kg / mu of high-nitrogen compound fertilizer after planting and survival, and 30kg / mu of high-nitrogen compound fertilizer in the rosette stage and the early stage of heart-wrapping.

[0063] The test area for each treatment was 0.5 mu. The effects of different treatments on Chinese cabbage yield, soluble sugar content and insect pests were investigated. The results are shown in Table 1:

[0064] Table 1 Chinese cabbage yield and pest survey

[0065]

[0066] It can be seen from the above survey results that the hemp water liquid fertilizer containing the insect repellent of this embodiment can effectively increase the yield of cabbage, and can have a good repellent effect on cabbage aphids and diamondback moths. In addition, the hemp water liquid fertilizer containing the insect repellent of this embodiment uses hemp water as the main component, is environmentally friendly, and effectively realizes the resource utilization of waste.

Claims

1. A liquid fertilizer containing insect repellent, characterized in that: The invention is prepared by fermenting the following ingredients in parts by weight through mixed strains: 800-1000 parts of pretreated hemp water, 50-80 parts of castor meal, 10-20 parts of mint leaves, 5-15 parts of star anise, 3-8 parts of pepper, 2-10 parts of chitosan and 1-5 parts of thickener. The mixed strains are prepared by mixing acclimated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohairi, Lactobacillus casei and Rhizopus oryzae mixed strains with photosynthetic bacterial strains.

2. The hemp water liquid fertilizer containing insect repellent according to claim 1, characterized in that, The pretreated hemp water is prepared by adding lime to the hemp water to adjust the pH to 5.5-6.

5.

3. The hemp water liquid fertilizer containing insect repellent according to claim 1, characterized in that, The preparation method of the mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae is as follows: Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae are inoculated into a domestication medium containing hemp water for domestication, respectively, wherein the domestication medium containing hemp water is a liquid medium, and 1-2 g / L of mint juice and 0.3-0.8 g / L of pepper water are added, and the concentration of hemp water is set to 12-14 g / L, 24-28 g / L, 48-56 g / L, and 96-112 g / L, respectively, and each concentration gradient is passaged 2-3 times, cultured for 24-48 hours, and then inoculated to the next concentration gradient after repeated domestication until domestication is completed, and then inoculated into an expansion medium, and cultured step by step, and the target of each level of culture is set to reach a composite viable bacteria number of 1×10 8 ~1×10 9 cfu / mL, and after the cultivation is completed, dry powder is prepared to obtain domesticated Bacillus thuringiensis, domesticated Bacillus subtilis, domesticated Bacillus mohaiwei, domesticated Lactobacillus casei and domesticated Rhizopus oryzae respectively, which are then mixed in a weight ratio of 5-10:5-10:1-2:2-3:1-2 to obtain a mixed strain of domesticated Bacillus thuringiensis, Bacillus subtilis, Bacillus mohaiwei, Lactobacillus casei and Rhizopus oryzae.

4. The hemp water liquid fertilizer containing insect repellent according to claim 3, characterized in that, The acclimation culture medium containing hemp water also contains the following ingredients: 250-300 g / L of glucose, 10-15 g / L of corn steep liquor, 10-15 g / L of potato juice, 0.1-0.2 g / L of MgSO4, and 0.3-1.0 g / L of NaCl.

5. The hemp water liquid fertilizer containing insect repellent according to claim 3, characterized in that, The expansion culture medium contains the following ingredients: 250-300 g / L glucose, 10-15 g / L corn steep liquor, 10-15 g / L potato juice, 15-22 g / L CaCO3, 0.5-1.0 g / L KH2PO4, 0.1-0.2 g / L MgSO4, and 0.3-1.0 g / L NaCl.

6. The hemp water liquid fertilizer containing the pest repellent according to claim 1, characterized in that, The weight ratio of the domesticated mixed strains of Bacillus thuringiensis, Bacillus subtilis, Bacillus mohairi, Lactobacillus casei and Rhizopus oryzae to the photosynthetic bacteria is 10-20:

1.

7. The method for preparing the liquid fertilizer containing insect repellent according to any one of claims 1 to 6, characterized in that: The following steps are involved: Add the mixed strains to the pretreated hemp water, mix and ferment for 3 to 4 days at 20 to 25°C, then add crushed mint leaves, crushed star anise and pepper, and castor meal, and continue to ferment for 7 to 12 days at 20 to 25°C. After the fermentation is completed, adjust the pH to 6.0 to 6.5, add chitosan and thickener, mix well, and obtain hemp water liquid fertilizer containing pest repellent.

8. Use of the hemp water liquid fertilizer according to any one of claims 1 to 6 or the hemp water liquid fertilizer prepared by the preparation method according to claim 7 in repelling vegetable pests.

9. The use according to claim 8, characterized in that: The vegetable pests are diamondback moth or vegetable aphid.

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