Composition and application thereof in promoting keratinocyte proliferation
Through a specific proportion of ceramide, glycerose esters and glycerol phospholipids, the problem of poor effect of ceramide repair products in the prior art in promoting the proliferation of keratinocytes is solved, and a significant cell proliferation effect is achieved.
Patent Information
- Application Number
- CN202510086351.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-20
- Publication Date
- 2025-04-29
AI Technical Summary
Existing ceramide repair products have limited effects in improving skin moisturizing and reducing wrinkles. The addition concentration is low and the variety is single, making it difficult to effectively promote the proliferation of keratinocytes.
The effect of promoting keratinocyte proliferation is verified by cellular experiments using a specific proportion of ceramide, glycerose esters and glycerol phospholipids.
The proliferation rate of keratinocytes is significantly improved, which is better than the effect of using ceramide or glycerol ester alone, and the synergistic effect of the three components used in combination on cell proliferation is more significant.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of skin care, in particular to a composition and application thereof in promoting the proliferation of keratinocytes. Background Art
[0002] Ceramide is mainly located in the epidermis of the skin, with a content of up to 40~50wt%. It is the main lipid in the epidermis and has functions such as moisturizing, signal transduction, and immune regulation. The secretion of ceramide in the skin decreases with age. Atopic dermatitis and other conditions can lead to a decrease in the content of ceramide in the stratum corneum, which in turn causes dry skin, wrinkles, and some skin inflammation. At present, ceramide has been widely used in a variety of cosmetics. Due to the low concentration of ceramide added and the relatively single type of ceramide added, ceramide repair products generally have a repair effect that needs to be improved.
[0003] Based on this, the technical solution of the present invention is proposed. Summary of the invention
[0004] One of the objects of the present invention is to provide a composition that can effectively promote the proliferation of keratinocytes.
[0005] The present invention provides a composition, comprising ceramide and glycerol glycolipid in a mass ratio of 1:(1-15), or further comprising glycerol phospholipid, wherein the mass ratio of ceramide, glycerol glycolipid and glycerol phospholipid is 1:(1-15):(1-10); The structural formula of the ceramide in the composition is as follows: Where R is C n H 2n+1 , n in R is independently a positive integer between 15 and 25; The structural formula of the glyceroglycolipid in the composition is as follows: Wherein R1 and R2 are each independently C n H 2n+1 , C n H 2n-1 , C n H 2n-3 or C n H 2n-5 , n in R1 and R2 is independently a positive integer between 15 and 23; R3 is (C6H 10 O5) n , n in R3 is a positive integer between 1 and 4; The structural formula of the glycerophospholipid in the composition is as follows: or ; Wherein R4 and R5 are each independently C n H 2n+1 , C n H 2n-1 , C n H 2n-3 or C n H 2n-5 , n in R4 and R5 is independently a positive integer between 15 and 23; R6 is OH or C6H 10 O5; R7 is OCH2CH2N + (CH3)3.
[0006] The present invention has found that when a specific ceramide and a specific glycerol glycolic acid ester are combined in a specific ratio, or when a specific ceramide and a specific glycerol glycolic acid ester are further combined with a specific glycerophospholipid in a specific ratio, the effect of promoting keratinocyte proliferation can be achieved.
[0007] The composition of the present invention preferably comprises ceramide and glycerol glycolipid in a mass ratio of 1:(1-10), or comprises ceramide, glycerol glycolipid and glycerophospholipid in a mass ratio of 1:(1-10):(1-7).
[0008] More preferably, the composition of the present invention comprises ceramide and glycerol glycolipid in a mass ratio of 1:(5-10), or comprises ceramide, glycerol glycolipid and glycerophospholipid in a mass ratio of 1:(4-5):(3.5-5).
[0009] More preferably, in the structural formula of ceramide, n in R is 16, 17 or 23; in the structural formula of glyceroglycolipid, R1 and R2 are both C n H 2n+1 , n=17 in R1 and R2; n=1 or 2 in R3; in the structural formula of glycerophospholipids, R4 and R5 are both: C n H 2n+1 , n in R4 and R5 = 15 or 17; or R4 and R5 are both: C n H 2n-3 , n=17 in R4 and R5.
[0010] More preferably, the composition of the present invention comprises ceramide and glycerol glycolipid in a mass ratio of 1:5, or comprises ceramide, glycerol glycolipid and glycerophospholipid in a mass ratio of 1:4:3.5 or 1:5:5.
[0011] The present invention also provides application of the composition in preparing cosmetics.
[0012] In the application of the present invention, the cosmetic can promote the proliferation of keratinocytes.
[0013] The composition of the present invention can be used as a component of repair cosmetics to achieve the effect of promoting the proliferation of keratinocytes.
[0014] The present invention also provides a cosmetic comprising the above composition.
[0015] The present invention also provides application of the composition in promoting proliferation of keratinocytes.
[0016] The present invention also provides a product for promoting the proliferation of keratinocytes, comprising the above composition.
[0017] The beneficial effects of the present invention are at least: The composition of the present invention has been found to have a significant effect in promoting the proliferation of keratinocytes through cell experiments, and can be added as a repairing ingredient in cosmetics. DETAILED DESCRIPTION
[0018] The preferred embodiments of the present invention will be described in detail below in conjunction with examples. It should be understood that the following examples are provided only for the purpose of illustration and are not intended to limit the scope of the present invention. Those skilled in the art may make various modifications and substitutions to the present invention without departing from the purpose and spirit of the present invention.
[0019] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples are commercially available or prepared according to conventional methods in the art unless otherwise specified.
[0020] Example 1 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6) and glycerol glycolipid (cas: 92457-02-8) in a mass ratio of 1:5.
[0021] The structure of ceramide is as follows: Where R:C n H 2n+1 , n=23.
[0022] The structure of glycerol ester is as follows: Where R1 and R2 are both: C n H 2n+1 , n=17; R3: (C6H 10 O5) n , n=2.
[0023] Example 2 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 2304-81-6) and glycerol glycolipid (cas: 41670-62-6) in a mass ratio of 1:5.
[0024] The structure of ceramide is as follows: Where R:C n H 2n+1 , n=17.
[0025] The structure of glycerol ester is as follows: Where R1 and R2 are both: C n H 2n+1 , n=17; R3: (C6H 10 O5) n , n=1.
[0026] Example 3 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6) and glycerol glycolipid (cas: 92457-02-8) in a mass ratio of 1:15.
[0027] Example 4 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6) and glycerol glycolipid (cas: 92457-02-8) in a mass ratio of 1:10.
[0028] Example 5 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6) and glycerol glycolipid (cas: 92457-02-8) in a mass ratio of 1:1.
[0029] Example 6 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6), glycerol glycolipid (cas: 92457-02-8) and glycerophospholipid (cas: 8002-43-5) in a mass ratio of 1:4:3.5. The structure of glycerophospholipid is as follows: Where R4 and R5 are both: C n H 2n+1 , n=15; R7= OCH2CH2N + (CH3)3.
[0030] Example 7 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 2304-18-6), glycerol glycolipid (cas: 41670-62-6) and glycerophospholipid (cas: 97281-52-2) in a mass ratio of 1:4:3.5. The structure of glycerophospholipid is as follows: Where R4 and R5 are both: C n H 2n+1 , n=17; R6= C6H 10 O5.
[0031] Example 8 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 67492-16-4), glycerol glycolipid (cas: 41670-62-6) and glycerophospholipid (cas: 475995-54-1) in a mass ratio of 1:4:3.5.
[0032] The structure of ceramide is as follows: Where R:C n H 2n+1 , n=16.
[0033] The structure of glycerophospholipids is as follows: Where R4 and R5 are both: C n H 2n-3 , n=17; R6= OH.
[0034] Example 9 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6), glycerol glycolipid (cas: 92457-02-8) and glycerophospholipid (cas: 8002-43-5) in a mass ratio of 1:5:5.
[0035] Example 10 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6), glycerol glycolipid (cas: 92457-02-8) and glycerophospholipid (cas: 8002-43-5) in a mass ratio of 1:10:7.
[0036] Embodiment 11 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6), glycerol glycolipid (cas: 92457-02-8) and glycerophospholipid (cas: 8002-43-5) in a mass ratio of 1:1:1.
[0037] Example 12 This embodiment provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6), glycerol glycolipid (cas: 92457-02-8) and glycerophospholipid (cas: 8002-43-5) in a mass ratio of 1:15:10.
[0038] Comparative Example 1 This comparative example provides a compound, which is ceramide (cas: 102917-80-6).
[0039] Comparative Example 2 This comparative example provides a compound, which is a glycerol sugar ester (cas: 92457-02-8).
[0040] Comparative Example 3 This comparative example provides a compound, which is glycerophospholipid (cas: 8002-43-5).
[0041] Comparative Example 4 This comparative example provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6) and glycerol glycolipid (cas: 92457-02-8) in a mass ratio of 1:20.
[0042] Comparative Example 5 This comparative example provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6), glycerol glycolipid (cas: 92457-02-8) and glycerophospholipid (cas: 8002-43-5) in a mass ratio of 1:20:15.
[0043] Comparative Example 6 This comparative example provides a composition containing ceramide, which contains ceramide (cas: 100403-19-8) and glycerol glycolipid (cas: 92457-02-8) in a mass ratio of 1:5.
[0044] Ceramide (cas: 100403-19-8) has the following structure: Where R:C n H 2n+1 , n=5.
[0045] Comparative Example 7 This comparative example provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6) and diglyceride (sugar-free) (cas: 25637-84-7) in a mass ratio of 1:5.
[0046] The structure of diglyceride (sugar-free) (cas: 25637-84-7) is as follows: Where R1 and R2 are both: C n H 2n-1 , n=17; R3=H.
[0047] Comparative Example 8 This comparative example provides a composition containing ceramide, which contains ceramide (cas: 102917-80-6), glycerol glycolipid (cas: 92457-02-8) and glycerophospholipid (cas: 39382-08-6) in a mass ratio of 1:4:3.5.
[0048] Glycerophospholipid (cas: 39382-08-6) has the following structure: Where R4 and R5 are both: C n H 2n-1 , n=7; R6= OCH2CH2N.
[0049] Comparative Example 9 This comparative example provides a compound, which comprises glycerol sugar ester (cas: 92457-02-8) and glycerophospholipid (cas: 8002-43-5) in a mass ratio of 4:3.5.
[0050] Example Cell experiment to verify the proliferation effect Blank group Human keratinocytes in the logarithmic growth phase were collected and the cell density was adjusted to be inoculated into 96-well plates, with each well containing 100 μL of culture medium. After culturing in a CO2 cell culture incubator for 24 hours, the culture medium was aspirated, the cells were washed twice with PBS buffer, and the cell culture medium was added. After that, the cells were cultured in a CO2 cell culture incubator for 48 hours, and then the MTT cell viability test was performed, and the absorbance OD value was read at 490 nm. The blank group was set up with 3 replicates. The relative cell proliferation rate was calculated.
[0051] Relative cell proliferation rate (%) = blank group OD 490 / Blank group OD 490,0h ×100%.
[0052] Note: The cell proliferation rate of the blank group at 0h was set as 100%.
[0053] Experimental Group Human keratinocytes in the logarithmic growth phase were collected, and the cell density was adjusted to be inoculated into a 96-well plate, with each well containing 100 μL of culture medium. After culturing in a CO2 cell culture incubator for 24 hours, the culture medium was aspirated, and the cells were washed twice with PBS buffer. Experimental groups 1-21 were set up, wherein experimental groups 1-12 were respectively added with the composition of Examples 1-12, and the concentration of the composition was 0.625 mg / mL, and experimental groups 13-21 were respectively added with the compound of Comparative Examples 1-9, and the concentration of the compound was 0.625 mg / mL. After that, the cells were cultured in a CO2 cell culture incubator for 48 hours, and then the MTT cell viability test was performed, and the absorbance OD value was read at 490 nm. Three replicates were set for each experimental group. The relative cell proliferation rate was calculated using the following formula: Relative cell proliferation rate (%) = Sample group OD 490 / Blank group OD 490,0h ×100%.
[0054] Note: The cell proliferation rate of the blank group at 0h was set as 100%.
[0055] The results are shown in Table 1.
[0056] Table 1 Proliferation of human keratinocytes in blank group and experimental group When ceramide and glycerol glycolic acid ester are used in combination (Examples 1-5), the two have a synergistic effect on the proliferation of human keratinocytes, and the proliferation effect of the combination is significantly better than the proliferation effect of the two alone. When ceramide, glycerol glycolic acid ester and glycerophospholipid are used in combination (Examples 6-12), the three can have a synergistic effect on the proliferation of human keratinocytes, and the effect of the combination is significantly better than the proliferation effect of the three alone. Secondly, the proliferation effect of the composition containing the three components on human keratinocytes is better than that of the composition containing the two components, which indicates that the three substances have a synergistic effect on the proliferation of human keratinocytes.
[0057] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.
Claims
1. A composition, characterized in that comprising ceramide and glycerol glycolipid in a mass ratio of 1:(1-15), or further comprising glycerol phospholipid, wherein the mass ratio of ceramide, glycerol glycolipid and glycerol phospholipid is 1:(1-15):(1-10); The structural formula of the ceramide in the composition is as follows: Where R is C n H 2n+1 , n in R is independently a positive integer between 15 and 25; The structural formula of the glyceroglycolipid in the composition is as follows: Wherein R1 and R2 are each independently C n H 2n+1 , C n H 2n-1 , C n H 2n-3 or C n H 2n-5 , n in R1 and R2 is independently a positive integer between 15 and 23; R3 is (C6H 10 O5) n , n in R3 is a positive integer between 1 and 4; The structural formula of the glycerophospholipid in the composition is as follows: or Wherein R4 and R5 are each independently C n H 2n+1 , C n H 2n-1 , C n H 2n-3 or C n H 2n-5 , n in R4 and R5 is independently a positive integer between 15 and 23; R6 is OH or C6H 10 O5; R7 is OCH2CH2N + (CH3)3.
2. The composition according to claim 1, characterized in that It comprises ceramide and glycerol glycolipid in a mass ratio of 1:(1-10), or comprises ceramide, glycerol glycolipid and glycerophospholipid in a mass ratio of 1:(1-10):(1-7).
3. The composition according to claim 2, characterized in that It includes ceramide and glycerol glycolipid in a mass ratio of 1:(5-10), or includes ceramide, glycerol glycolipid and glycerophospholipid in a mass ratio of 1:(4-5):(3.5-5).
4. The composition according to any one of claims 1 to 3, characterized in that In the structural formula of ceramide, n in R is 16, 17 or 23; In the structural formula of glyceroglycolipid, R1 and R2 are both C n H 2n+1 , n=17 in R1 and R2; n=1 or 2 in R3; In the structural formula of glycerophospholipids, R4 and R5 are both: C n H 2n+1 , n in R4 and R5 = 15 or 17; or R4 and R5 are both: C n H 2n-3 , n=17 in R4 and R5.
5. The composition according to claim 4, characterized in that It comprises ceramide and glycerol glycolipid in a mass ratio of 1:5, or comprises ceramide, glycerol glycolipid and glycerophospholipid in a mass ratio of 1:4:3.5 or 1:5:
5.
6. Use of the composition according to any one of claims 1 to 5 in the preparation of cosmetics.
7. The use according to claim 6, characterized in that: The cosmetics can promote the proliferation of keratinocytes.
8. A cosmetic, characterized in that: The invention comprises the composition according to any one of claims 1 to 5.
9. Use of the composition according to any one of claims 1 to 5 in promoting keratinocyte proliferation.
10. A product for promoting keratinocyte proliferation, characterized in that: The invention comprises the composition according to any one of claims 1 to 5.