Adsorbent for removing myoglobin in blood and preparation method thereof

Abstract

The invention relates to the technical field of blood purification, and particularly discloses an adsorbent for removing myoglobin in blood and a preparation method of the adsorbent. The adsorbent for removing the myoglobin in the blood is prepared by grafting double bonds, imprinting and crosslinking, eluting template molecules, imprinting and then modifying, the prepared adsorbent can specifically adsorb the myoglobin and can be repeatedly used for more than 5 times through desorption, the adsorption rate of the myoglobin can still reach more than 80%, and the adsorbent can be used for removing the myoglobin in the blood. Repeated recycling of the material is achieved, and the material can be used for treating hypermyohemoglobin through blood perfusion or plasma adsorption.

Description

Technical Field

[0001] The present invention relates to the technical field of blood purification, and in particular to an adsorbent for removing myoglobin in blood and a preparation method thereof. Background Art

[0002] Myoglobin (Mb) is a small molecule pigment protein composed of globin and heme, with a molecular weight of about 17-18kDa. It is mainly found in cardiac and skeletal muscle cells and is responsible for the storage and transport of oxygen. The molecular structure of myoglobin is similar to that of hemoglobin, but it has a higher oxygen affinity and can quickly release oxygen in a hypoxic environment to maintain cell metabolism. Under normal circumstances, the concentration of myoglobin in the blood is extremely low (<87μg / L), but in diseases such as myocardial infarction and rhabdomyolysis, a large amount of myoglobin is released into the blood due to cell membrane rupture. Myoglobin induces apoptosis of renal tubular epithelial cells through endoplasmic reticulum stress, leading to serious complications such as acute kidney injury.

[0003] Among the current blood purification adsorption materials, the more commonly used adsorption materials are activated carbon, resins and some high molecular polymers, which mainly remove toxins from the blood through physical adsorption or ion exchange. However, myoglobin has a small molecular weight, an isoelectric point (pI≈6.9) close to the plasma pH environment, and its surface charge distribution overlaps with plasma proteins such as albumin, resulting in extremely low selectivity of traditional adsorbents for it.

[0004] For example, Chinese patent document CN202311507220.9 discloses an adsorbent for removing myoglobin and a preparation method thereof, comprising the following steps: styrene monomers, polyvinyl cross-linking agents, halogen-containing monomers, initiators and porogens are mutually dissolved to obtain an oil phase, a dispersant is dissolved in water to obtain an aqueous phase, the aqueous phase and the oil are mutually dissolved to obtain an oil-in-water suspension, the oil-in-water suspension is subjected to a suspension polymerization reaction to obtain a styrene macroporous resin containing halogenated hydrocarbons; the styrene macroporous resin containing halogenated hydrocarbons and an amino acid containing a benzene ring are subjected to a nucleophilic substitution reaction to graft the amino acid onto the styrene macroporous resin to obtain an adsorbent; the prepared adsorbent has a high clearance rate for myoglobin, but the above patent does not involve the adsorption of hemoglobin in the blood. Summary of the invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide an adsorbent for removing myoglobin from blood and a preparation method thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical scheme:

[0007] In a first aspect, the present invention provides a method for preparing an adsorbent for removing myoglobin from blood, comprising the following steps:

[0008] S1. Preparation of vinyl grafted silica

[0009] The silica microspheres are dispersed in an ethanol aqueous solution, and then a vinyl silane coupling agent is added thereto, and the mixture is stirred for reaction. After the reaction is completed, the mixture is filtered, washed, and dried to obtain vinyl grafted silica.

[0010] In this step, the particle size of the selected silica microspheres is 100 μm-1 mm.

[0011] In this step, the mass ratio of silica microspheres to vinyl silane coupling agent is 10-15:1-5. In some embodiments of the present invention, for example, 10:1, 10:2, 10:3, 10:4, 10:5, 12:1, 12:2, 12:3, 12:4, 12:5, 15:1, 15:2, 15:3, 15:4, 15:5 can be selected, but it is not limited to the listed values, and other unlisted values ​​within the numerical range are also applicable.

[0012] In this step, the vinyl silane coupling agent is selected from vinyl trimethoxy silane, vinyl triethoxy silane or vinyl tri(β-methoxyethoxy) silane. It should be noted that other silane coupling agents containing double bonds not listed are also applicable.

[0013] In this step, the silica microspheres are treated with a vinyl silane coupling agent to introduce carbon-carbon double bonds on the surface of the silica microspheres, which is beneficial to the subsequent reaction.

[0014] S2. Preparation of modified silicon dioxide

[0015] The vinyl grafted silica is dispersed in deionized water, and then myoglobin is added thereto and stirred to dissolve, followed by adding itaconic acid, a crosslinking agent and an initiator, and a crosslinking reaction is carried out under a nitrogen atmosphere. After the crosslinking reaction is completed, the template molecule myoglobin is filtered, eluted, and dried to obtain modified silica.

[0016] In this step, the mass ratio of vinyl grafted silica, deionized water, myoglobin, itaconic acid, crosslinking agent and initiator is 4-8:100-150:0.3-0.6:1-2:0.5-1:0.2-0.4.

[0017] More specifically, the cross-linking agent is selected from N,N'-methylenebisacrylamide.

[0018] More specifically, the initiator is selected from persulfates, for example, sodium persulfate, potassium persulfate or ammonium persulfate.

[0019] In this step, the temperature of the cross-linking reaction is 60-80°C, for example, 60°C, 65°C, 70°C, 75°C, and 80°C can be selected; the time of the cross-linking reaction is 2-4h, for example, 2h, 2.5h, 3h, 3.5h, and 4h can be selected, but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0020] In this step, the solvent selected for eluting the template molecule is an acid solution, and the acid solution is an inorganic acid solution, for example, a sulfuric acid solution, a nitric acid solution or a hydrochloric acid solution can be selected, and the concentration of the acid solution is 0.1-0.5 mol / L, for example, 0.1 mol / L, 0.2 mol / L, 0.3 mol / L, 0.4 mol / L, 0.5 mol / L can be selected, but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0021] In this step, itaconic acid is selected as the functional monomer for preparing the myoglobin molecular imprinting polymer, so that the prepared molecular imprinting polymer has hydrophilicity. At the same time, the itaconic acid molecule contains two carboxyl groups, which can provide more adsorption sites compared with acrylic acid. The carboxyl groups form hydrogen bonds with the active groups in the myoglobin molecules to fix the myoglobin molecules on the surface of the silica microspheres. In addition, the hydrogen bonds formed are easy to break in an acidic environment, which is conducive to eluting the template molecules under acidic conditions, effectively solving the problem of traditional imprinting materials that the template molecules are difficult to elute due to the excessively deep embedding of the sites.

[0022] S3. Preparation of adsorbent

[0023] The modified silica is dispersed in deionized water, and then EDC and NHS are added thereto, stirred for reaction, and then 4-aminophenylboronic acid is added, heated and stirred for reaction, and after the reaction is completed, the adsorbent is obtained by filtering, washing and drying.

[0024] In this step, the mass ratio of modified silica, deionized water, EDC, NHS and 4-aminophenylboronic acid is 4-8:100:0.5-1:0.5-1:0.8-1.2.

[0025] In this step, the temperature of the heating and stirring reaction is 50-75°C, for example, 50°C, 55°C, 60°C, 65°C, 70°C, and 75°C can be selected; the time of the heating and stirring reaction is 1-3h, for example, 1h, 1.5h, 2h, 2.5h, and 3h can be selected, but it is not limited to the listed values, and other values ​​not listed within the numerical range are also applicable.

[0026] In this step, a post-imprinting modification scheme is adopted to perform site-specific modification in the imprinting recognition cavity. The carboxyl groups in the modified silica are first activated using EDC and NHS, and then 4-aminophenylboronic acid is grafted into the imprinting recognition cavity through chemical cross-linking of the carboxyl groups and amino groups, further enhancing the affinity and recognition selectivity of the imprinting site for the template molecule.

[0027] In a second aspect, the present invention provides an adsorbent for removing myoglobin from blood prepared by the above preparation method.

[0028] In a third aspect, the present invention also provides use of the above-mentioned adsorbent in the specific adsorption of myoglobin.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] (1) The present invention uses silica microspheres as adsorption carriers, and prepares an adsorbent for removing myoglobin from blood by grafting double bonds, imprinting cross-linking and eluting template molecules, and modifying after imprinting. The adsorbent prepared in this way can specifically adsorb myoglobin and can be reused more than 5 times through desorption, and can be used for blood perfusion or plasma adsorption to treat hypermyoglobinemia.

[0031] (2) The present invention selects itaconic acid as the functional monomer for preparing the myoglobin molecular imprinting polymer, so that the prepared molecular imprinting polymer has hydrophilicity. At the same time, the itaconic acid molecule contains two carboxyl groups, which can provide more adsorption sites compared with acrylic acid. The carboxyl groups form hydrogen bonds with the active groups in the myoglobin molecules to fix the myoglobin molecules on the surface of the silica microspheres. In addition, the hydrogen bonds formed are easy to break in an acidic environment, which is conducive to eluting the template molecules under acidic conditions, effectively solving the problem of traditional imprinting materials that the template molecules are difficult to elute due to the excessive embedding of the sites.

[0032] (3) The present invention adopts a post-imprinting modification scheme to perform site-specific modification in the imprinting recognition cavity. The carboxyl groups in the modified silica are first activated using EDC and NHS, and then 4-aminophenylboronic acid is grafted into the imprinting recognition cavity through chemical cross-linking of the carboxyl groups and amino groups, thereby further enhancing the affinity and recognition selectivity of the imprinting site for the template molecule. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 The adsorption performance test results of different groups of adsorbents provided by the present invention are shown in FIG.

[0034] Figure 2 This is a graph showing the test results of the reusability of the adsorbent provided in Example 1 of the present invention. DETAILED DESCRIPTION

[0035] The present invention is further described in detail below through specific preferred embodiments, but the present invention is not limited to the following embodiments.

[0036] It should be noted that, unless otherwise specified, the chemical reagents involved in the present invention were purchased through commercial channels.

[0037] The average particle size of the silica microspheres used in the embodiment of the present invention is 500 μm, and the CAS number of myoglobin is 100684-32-0.

[0038] Example 1

[0039] A method for preparing an adsorbent for removing myoglobin from blood comprises the following steps:

[0040] S1. Disperse 10 g of silica microspheres in 100 mL of 80 wt% ethanol aqueous solution, then add 2 g of vinyl triethoxysilane, stir and react at room temperature for 2 h, and after the reaction is completed, filter, wash and dry to obtain vinyl grafted silica;

[0041] S2, 4g of vinyl grafted silica was dispersed in 100g of deionized water, and then 0.3g of myoglobin was added thereto, stirred to dissolve, and then 1g of itaconic acid, 0.5g of cross-linking agent N,N'-methylenebisacrylamide and 0.2g of initiator ammonium persulfate were added, and a cross-linking reaction was carried out under a nitrogen atmosphere at a temperature of 60°C and a time of 4h. After the cross-linking reaction was completed, the mixture was filtered, and the template molecules were eluted with a 0.1mol / L hydrochloric acid solution, and then dried to obtain modified silica;

[0042] S3. Disperse 4 g of modified silica in 100 g of deionized water, then add 0.5 g of EDC and 0.5 g of NHS, stir and react at room temperature for 1 h, then add 0.8 g of 4-aminophenylboronic acid, heat and stir at 50°C for 3 h, and after the reaction is completed, filter, wash and dry to obtain an adsorbent.

[0043] Example 2

[0044] A method for preparing an adsorbent for removing myoglobin from blood comprises the following steps:

[0045] S1. Disperse 15 g of silica microspheres in 100 mL of 80 wt% ethanol aqueous solution, then add 5 g of vinyl triethoxysilane, stir and react at room temperature for 2 h, and after the reaction is completed, filter, wash and dry to obtain vinyl grafted silica;

[0046] S2, 8g of vinyl grafted silica was dispersed in 150g of deionized water, and then 0.6g of myoglobin was added thereto, stirred and dissolved, and then 2g of itaconic acid, 1g of cross-linking agent N,N'-methylenebisacrylamide and 0.4g of initiator ammonium persulfate were added, and a cross-linking reaction was carried out under a nitrogen atmosphere at a temperature of 80°C and a time of 2h. After the cross-linking reaction was completed, the mixture was filtered, and the template molecules were eluted with a 0.1mol / L hydrochloric acid solution, and then dried to obtain modified silica;

[0047] S3. Disperse 8 g of modified silica in 100 g of deionized water, then add 1 g of EDC and 1 g of NHS, stir and react at room temperature for 1 h, then add 1.5 g of 4-aminophenylboronic acid, heat and stir and react at 75°C for 1 h. After the reaction is completed, filter, wash and dry to obtain an adsorbent.

[0048] Example 3

[0049] A method for preparing an adsorbent for removing myoglobin from blood comprises the following steps:

[0050] S1. Disperse 12 g of silica microspheres in 100 mL of 80 wt% ethanol aqueous solution, then add 3 g of vinyl trimethoxysilane, stir and react at room temperature for 2 h, and after the reaction is completed, filter, wash and dry to obtain vinyl grafted silica;

[0051] S2, 6g of vinyl grafted silica was dispersed in 120g of deionized water, and then 0.5g of myoglobin was added thereto, stirred to dissolve, and then 1.5g of itaconic acid, 0.8g of cross-linking agent N,N'-methylenebisacrylamide and 0.3g of initiator ammonium persulfate were added, and a cross-linking reaction was carried out under a nitrogen atmosphere. The temperature of the cross-linking reaction was 70°C, and the time of the cross-linking reaction was 3h. After the cross-linking reaction was completed, the mixture was filtered, and the template molecules were eluted with a 0.1mol / L hydrochloric acid solution, and then dried to obtain modified silica;

[0052] S3. Disperse 6 g of modified silica in 100 g of deionized water, then add 0.8 g of EDC and 0.8 g of NHS, stir and react at room temperature for 1 h, then add 1 g of 4-aminophenylboronic acid, heat and stir at 60°C for 2 h, and after the reaction is completed, filter, wash and dry to obtain an adsorbent.

[0053] Example 4

[0054] A method for preparing an adsorbent for removing myoglobin from blood comprises the following steps:

[0055] S1. Disperse 12 g of silica microspheres in 100 mL of 80 wt% ethanol aqueous solution, then add 3 g of vinyl tri(β-methoxyethoxy)silane, stir and react at room temperature for 2 h. After the reaction is completed, filter, wash and dry to obtain vinyl grafted silica;

[0056] S2, 5g of vinyl grafted silica was dispersed in 120g of deionized water, and then 0.4g of myoglobin was added thereto, stirred and dissolved, and then 1.5g of itaconic acid, 1g of cross-linking agent N,N'-methylenebisacrylamide and 0.3g of initiator ammonium persulfate were added, and a cross-linking reaction was carried out under a nitrogen atmosphere at a temperature of 70°C and a time of 3h. After the cross-linking reaction was completed, the mixture was filtered, and the template molecules were eluted with a 0.1mol / L hydrochloric acid solution, and then dried to obtain modified silica;

[0057] S3. Disperse 5 g of modified silica in 100 g of deionized water, then add 0.6 g of EDC and 0.6 g of NHS, stir and react at room temperature for 1 h, then add 1 g of 4-aminophenylboronic acid, heat and stir and react at 75°C for 1 h. After the reaction is completed, filter, wash and dry to obtain an adsorbent.

[0058] Comparative Example 1

[0059] A method for preparing an adsorbent for removing myoglobin from blood comprises the following steps:

[0060] S1. Disperse 10 g of silica microspheres in 100 mL of 80 wt% ethanol aqueous solution, then add 2 g of vinyl triethoxysilane, stir and react at room temperature for 2 h, and after the reaction is completed, filter, wash and dry to obtain vinyl grafted silica;

[0061] S2, dispersing 4g of vinyl grafted silica in 100g of deionized water, then adding 1g of itaconic acid, 0.5g of crosslinking agent N,N'-methylenebisacrylamide and 0.2g of initiator ammonium persulfate, and carrying out crosslinking reaction under nitrogen atmosphere, the temperature of crosslinking reaction is 60°C, the time of crosslinking reaction is 4h, after the crosslinking reaction is completed, filtering, washing and drying to obtain modified silica;

[0062] S3. Disperse 4 g of modified silica in 100 g of deionized water, then add 0.5 g of EDC and 0.5 g of NHS, stir and react at room temperature for 1 h, then add 0.8 g of 4-aminophenylboronic acid, heat and stir at 50°C for 3 h, and after the reaction is completed, filter, wash and dry to obtain an adsorbent.

[0063] Compared with Example 1, in Comparative Example 1, myoglobin was not added in step S2.

[0064] Comparative Example 2

[0065] A method for preparing an adsorbent for removing myoglobin from blood comprises the following steps:

[0066] S1. Disperse 10 g of silica microspheres in 100 mL of 80 wt% ethanol aqueous solution, then add 2 g of vinyl triethoxysilane, stir and react at room temperature for 2 h, and after the reaction is completed, filter, wash and dry to obtain vinyl grafted silica;

[0067] S2. Disperse 4g of vinyl grafted silica in 100g of deionized water, then add 0.3g of myoglobin, stir to dissolve, then add 1g of itaconic acid, 0.5g of cross-linking agent N,N'-methylenebisacrylamide and 0.2g of initiator ammonium persulfate, and carry out cross-linking reaction under nitrogen atmosphere. The temperature of the cross-linking reaction is 60°C, and the time of the cross-linking reaction is 4h. After the cross-linking reaction is completed, filter, elute the template molecule with 0.1mol / L hydrochloric acid solution, and then dry to obtain an adsorbent.

[0068] Compared with Example 1, in Comparative Example 2, step S3 is omitted, and no grafting 4-aminophenylboronic acid treatment is performed.

[0069] Comparative Example 3

[0070] A method for preparing an adsorbent for removing myoglobin from blood comprises the following steps:

[0071] S1. Disperse 10 g of silica microspheres in 100 mL of 80 wt% ethanol aqueous solution, then add 2 g of vinyl triethoxysilane, stir and react at room temperature for 2 h, and after the reaction is completed, filter, wash and dry to obtain vinyl grafted silica;

[0072] S2, 4g of vinyl grafted silica was dispersed in 100g of deionized water, and then 0.3g of myoglobin was added thereto, stirred and dissolved, and then 1g of acrylic acid, 0.5g of cross-linking agent N,N'-methylenebisacrylamide and 0.2g of initiator ammonium persulfate were added, and a cross-linking reaction was carried out under a nitrogen atmosphere at a temperature of 60°C and a time of 4h. After the cross-linking reaction was completed, the mixture was filtered, and the template molecules were eluted with a 0.1mol / L hydrochloric acid solution, and then dried to obtain modified silica;

[0073] S3. Disperse 4 g of modified silica in 100 g of deionized water, then add 0.5 g of EDC and 0.5 g of NHS, stir and react at room temperature for 1 h, then add 0.8 g of 4-aminophenylboronic acid, heat and stir at 50°C for 3 h, and after the reaction is completed, filter, wash and dry to obtain an adsorbent.

[0074] Compared with Example 1, in Comparative Example 3, acrylic acid is used to replace itaconic acid in step S2.

[0075] Comparative Example 4

[0076] A method for preparing an adsorbent for removing myoglobin from blood comprises the following steps:

[0077] S1. Disperse 10 g of silica microspheres in 100 mL of 80 wt% ethanol aqueous solution, then add 2 g of vinyl triethoxysilane, stir and react at room temperature for 2 h, and after the reaction is completed, filter, wash and dry to obtain vinyl grafted silica;

[0078] S2, 4g of vinyl grafted silica was dispersed in 100g of deionized water, and then 0.3g of myoglobin was added thereto, stirred to dissolve, and then 1g of itaconic acid, 0.5g of cross-linking agent N,N'-methylenebisacrylamide and 0.2g of initiator ammonium persulfate were added, and a cross-linking reaction was carried out under a nitrogen atmosphere at a temperature of 60°C and a time of 4h. After the cross-linking reaction was completed, the mixture was filtered, and the template molecules were eluted with a 0.1mol / L hydrochloric acid solution, and then dried to obtain modified silica;

[0079] S3. Disperse 4 g of modified silica in 100 g of deionized water, then add 0.5 g of EDC and 0.5 g of NHS, stir and react at room temperature for 1 h, then add 0.8 g of phenylalanine, heat and stir and react at 50°C for 3 h. After the reaction is completed, filter, wash and dry to obtain the adsorbent.

[0080] Compared with Example 1, in Comparative Example 4, phenylalanine is used to replace 4-aminophenylboronic acid in step S3.

[0081] The adsorbents prepared in Examples 1-2 and Comparative Examples 1-4 were tested for adsorption performance, and the specific steps were as follows:

[0082] 0.1 g of the adsorbent prepared in Examples 1-2 and Comparative Examples 1-4 was added to 10 mL of plasma, the concentration of myoglobin in the plasma was 1 mg / L, and the concentration of hemoglobin was 1 mg / L. The mixture was adsorbed at 37 ° C for 1 h and filtered. The concentrations of myoglobin and hemoglobin in the plasma after adsorption were tested, and the adsorption rate was calculated, where the adsorption rate = [(C0-C1) / C0] × 100%, C0 is the concentration of the substance in the solution before adsorption, and C1 is the concentration of the substance in the solution after adsorption. The results are as follows: Figure 1 As shown, from Figure 1 It can be seen that the adsorbent prepared in the embodiment of the present invention has good selective adsorption to myoglobin, and the adsorption rate to myoglobin can reach more than 90%, while the adsorption rate to hemoglobin is only about 2%.

[0083] The adsorbent prepared in Example 1 of the present invention was tested for reusability. 0.1 g of the adsorbent prepared in Example 1 was added to 10 mL of plasma, the concentration of myoglobin in the plasma was 1 mg / L, and the concentration of hemoglobin was 1 mg / L. The adsorbent was shaken and adsorbed at 37°C for 1 hour, filtered, and the concentration of myoglobin in the plasma after adsorption was tested. The adsorbent was then analyzed using a 0.1 mol / L hydrochloric acid solution. After washing and drying, the above experimental steps were repeated. The adsorbent used for the first time was recorded as the first time, and it was reused 5 times. The results are as follows: Figure 2 As shown, from Figure 2 It can be seen that the adsorbent prepared in the embodiment of the present invention can be reused 5 times through desorption, and the adsorption rate of myoglobin can still reach more than 80%, realizing the repeated recycling of materials.

[0084] Finally, it should be noted that the above embodiments do not limit the present invention in any form. For those skilled in the art, some modifications and improvements can be made to the present invention. Therefore, any modification or improvement made without departing from the spirit of the present invention belongs to the scope of protection claimed in the present invention.

Claims

1. A method for preparing an adsorbent for removing myoglobin from blood, characterized in that: The steps include: S1, dispersing silica microspheres in an ethanol aqueous solution, then adding a vinyl silane coupling agent thereto, stirring for reaction, and after the reaction is completed, filtering, washing, and drying to obtain vinyl grafted silica; S2, dispersing the vinyl grafted silica in deionized water, then adding myoglobin thereto, stirring to dissolve, then adding itaconic acid, a crosslinking agent and an initiator, and performing a crosslinking reaction under a nitrogen atmosphere. After the crosslinking reaction is completed, filtering, eluting the template molecule myoglobin, and drying to obtain modified silica; S3, dispersing the modified silica in deionized water, then adding EDC and NHS thereto, stirring to react, then adding 4-aminophenylboronic acid, heating and stirring to react, after the reaction is completed, filtering, washing and drying to obtain an adsorbent.

2. The preparation method according to claim 1, characterized in that: In step S1, the mass ratio of silica microspheres to vinyl silane coupling agent is 10-15:1-5.

3. The preparation method according to claim 1, characterized in that: In step S1, the vinyl silane coupling agent is selected from vinyl trimethoxy silane, vinyl triethoxy silane or vinyl tri (β-methoxyethoxy) silane.

4. The preparation method according to claim 1, characterized in that: In step S2, the mass ratio of vinyl grafted silica, deionized water, myoglobin, itaconic acid, crosslinking agent and initiator is 4-8:100-150:0.3-0.6:1-2:0.5-1:0.2-0.

4.

5. The preparation method according to claim 1, characterized in that: In step S2, the cross-linking agent is selected from N,N'-methylenebisacrylamide, and the initiator is selected from persulfate.

6. The preparation method according to claim 1, characterized in that: In step S2, the temperature of the cross-linking reaction is 60-80° C., and the time of the cross-linking reaction is 2-4 hours.

7. The preparation method according to claim 1, characterized in that: In step S3, the mass ratio of modified silica, deionized water, EDC, NHS and 4-aminophenylboronic acid is 4-8:100:0.5-1:0.5-1:0.8-1.

2.

8. The preparation method according to claim 1, characterized in that: In step S3, the temperature of the heating and stirring reaction is 50-75° C., and the time of the heating and stirring reaction is 1-3 h.

9. An adsorbent for removing myoglobin from blood prepared by the preparation method according to any one of claims 1 to 8.

10. Use of the adsorbent according to claim 9 in specific adsorption of myoglobin.

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