Nutrient for improving quality of cornu cervi pantotrichum and preparation method thereof

Abstract

The invention belongs to the technical field of feed additives, and particularly relates to a nutrient for improving the quality of pilose antler and a preparation method thereof. The nutrient comprises the following raw materials in parts by weight: 15-20 parts of aged zeolite, 6-9 parts of modified montmorillonite, 1-5 parts of oyster polypeptide and 0.2-0.5 part of vitamin. The preparation process of the modified montmorillonite comprises the following steps: adding montmorillonite into a zinc chloride solution, then adding octadecyl trimethyl ammonium chloride to obtain a mixed solution, adjusting the pH value of the mixed solution, carrying out a stirring reaction, and carrying out purification and calcination to obtain pretreated montmorillonite; adding the pretreated montmorillonite into a mixed solution of ethanol and water, then adding a silane coupling agent, and carrying out a heating reaction to obtain silane coupling agent modified montmorillonite; adding the silane coupling agent modified montmorillonite into DMF (Dimethyl Formamide), then adding lauric acid, DCC (Dimethyl Cellulose) and DMAP (Dimethyl Ammonium Phosphate), and carrying out heating reaction to obtain the modified montmorillonite. All the components of the nutrient are matched for use, so that the yield and quality of pilose antler can be effectively improved.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and particularly relates to a nutrient for improving the quality of velvet antlers and a preparation method thereof. Background Art

[0002] Deer antler refers to the unossified and hairy young antlers of male sika deer or red deer, and is a precious Chinese medicinal material. Deer antler contains phospholipids, glycolipids, gelatin, hormones, fatty acids, amino acids, proteins, calcium, phosphorus, magnesium, sodium and other ingredients, of which amino acids account for more than half of the total ingredients. Deer antler is warm but not dry, and is a traditional Chinese medicine ingredient that has the effect of invigorating and improving the body. In addition, deer antler also has a high economic value, which has also enabled the rapid development of the sika deer breeding industry.

[0003] By visiting a large number of farmers, it was found that during the breeding process of sika deer, problems such as rumen food accumulation, decreased appetite, deer weight loss, and low antler production and quality were prone to occur, which significantly affected the antler production and quality of male deer. In order to give full play to the antler production performance of sika deer, reduce breeding costs, reduce the occurrence of diseases, and improve the antler production performance and antler quality of sika deer in daily production, the present invention provides a new feed additive. Summary of the invention

[0004] The first object of the present invention is to provide a nutrient for improving the quality of velvet antlers, wherein the nutrient can improve the yield and quality of velvet antlers.

[0005] The second object of the present invention is to provide a method for preparing nutrients for improving the quality of velvet antlers, with simple preparation steps.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is:

[0007] A nutrient for improving the quality of velvet antlers, comprising the following raw materials in parts by weight: 15-20 parts of aged zeolite, 6-9 parts of modified montmorillonite, 1-5 parts of oyster polypeptide, and 0.2-0.5 parts of vitamins;

[0008] The preparation process of the modified montmorillonite is as follows:

[0009] (1) adding montmorillonite to a zinc chloride solution, and then adding octadecyltrimethylammonium chloride to obtain a mixed solution; adjusting the pH of the mixed solution, and then stirring the mixed solution for reaction; after the reaction is completed, filtering, washing, drying, and calcining to obtain pretreated montmorillonite;

[0010] (2) adding the pretreated montmorillonite of step (1) to a mixed solution of ethanol and water, and then adding a silane coupling agent to carry out a heating reaction, and after the reaction is completed, purifying to obtain a silane coupling agent modified montmorillonite;

[0011] (3) adding the silane coupling agent modified montmorillonite of step (2) into DMF, and then adding lauric acid, DCC and DMAP for heating reaction. After the reaction is completed, the modified montmorillonite is purified to obtain the modified montmorillonite.

[0012] Furthermore, in step (3), the usage ratio of the silane coupling agent modified montmorillonite, lauric acid, DCC, DMAP and DMF in step (2) is 10 g: (25-35) g: (8-12) g: (4-9) g: 100 mL.

[0013] Furthermore, the temperature of the heating reaction in step (3) is 75-85° C., and the heating reaction time is 2-3 h.

[0014] Furthermore, in step (1), the mass ratio of montmorillonite, zinc chloride and octadecyltrimethylammonium chloride is 100:(4-12):(1-5); the concentration of the zinc chloride solution is 0.01-0.03 g / mL; and the pH of the mixed solution is adjusted to 7.

[0015] Furthermore, in step (1), the stirring reaction temperature is 75-85°C, and the stirring reaction time is 2-3h; the calcination temperature is 450-550°C, and the calcination time is 4-6h.

[0016] Furthermore, in step (2), the amount ratio of the pretreated montmorillonite, silane coupling agent and mixed solution of step (1) is 1g: (0.5-2)g: (120-200)mL; the silane coupling agent is 3-aminopropyltriethoxysilane; and the volume ratio of ethanol to water in the mixed solution of ethanol and water is 3:1.

[0017] Furthermore, the temperature of the heating reaction in step (2) is 45-55° C., and the heating reaction time is 10-12 h.

[0018] Furthermore, the preparation process of the oyster polypeptide is as follows:

[0019] According to the material-liquid ratio of 1g: (2-4)mL, water is added to the oyster meat homogenate to adjust the pH to 1-3; then pepsin is added according to 0.5% of the mass of the oyster meat homogenate, and enzymatic hydrolysis is carried out at 37-40°C for 3-4h. After the enzyme is inactivated in a boiling water bath for 10min, the supernatant is collected; after ultrafiltration of the supernatant, an oyster polypeptide with a molecular weight of less than 10kDa is obtained, which is obtained after drying.

[0020] Furthermore, the vitamin is vitamin C or vitamin E.

[0021] Furthermore, the preparation process of the aged zeolite is as follows: the zeolite is heated at 1200° C. for 2 h, cooled to room temperature, and then aged at 35° C. for 9 d to obtain the aged zeolite.

[0022] The method for preparing the above-mentioned nutrient for improving the quality of velvet antler comprises the following steps:

[0023] The raw materials are mixed according to the weight ratio.

[0024] Compared with the prior art, the beneficial effects of the present invention are mainly:

[0025] The nutrients provided by the present invention include aged zeolite, modified montmorillonite, oyster polypeptide and other ingredients, and the combination of the ingredients can effectively improve the yield and quality of velvet antlers. Specifically, montmorillonite has better adsorption, dispersibility, biocompatibility and biological activity after modification. It can adsorb harmful substances in the rumen and intestines of deer when used together with aged zeolite, and has the effects of deer removal and sterilization, and provides deer with trace elements necessary for growth, thereby improving the immune function of deer, improving intestinal health, promoting the absorption of nutrients in feed by deer, improving feed conversion rate, and significantly improving the yield and quality of velvet antlers. Oyster polypeptides have a variety of biological activities, such as anti-oxidation, anti-inflammatory and immunomodulation, etc. During the growth of velvet antlers, they improve the yield and quality of velvet antlers by improving immunity. DETAILED DESCRIPTION

[0026] The technical scheme of the present invention is further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only used to illustrate the present invention and should not be regarded as limiting the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0027] Example

[0028] Example 1

[0029] This embodiment provides a nutrient for improving the quality of velvet antlers. The nutrient is composed of the following raw materials in parts by weight: 18 parts of aged zeolite, 7 parts of modified montmorillonite, 3 parts of oyster polypeptide, and 0.4 parts of vitamin C.

[0030] Among them, the preparation process of modified montmorillonite is as follows:

[0031] (1) According to the mass ratio of montmorillonite, zinc chloride and octadecyltrimethylammonium chloride of 100:10:3, montmorillonite is added to a zinc chloride solution with a concentration of 0.02 g / mL, and then octadecyltrimethylammonium chloride is added to obtain a mixed solution; the pH of the mixed solution is adjusted to 7, and the mixture is stirred at 80° C. for 2.5 hours. After the reaction is completed, the mixture is filtered, washed, dried, and calcined at 500° C. for 5 hours to obtain a pretreated montmorillonite;

[0032] (2) adding the pretreated montmorillonite of step (1) to a mixed solution of ethanol and water (v / v=3:1) according to the amount ratio of the pretreated montmorillonite, 3-aminopropyltriethoxysilane and the mixed solution of 1 g:1 g:150 mL, then adding 3-aminopropyltriethoxysilane, heating at 50° C. for reaction for 11 h, filtering, washing and drying after the reaction is completed to obtain silane coupling agent modified montmorillonite;

[0033] (3) According to the amount ratio of silane coupling agent modified montmorillonite, lauric acid, DCC, DMAP and DMF in step (2) of 10g:30g:10g:6g:100mL, the silane coupling agent modified montmorillonite in step (2) is added to DMF, and then lauric acid, DCC and DMAP are added, and the mixture is heated at 80°C for reaction for 2.5h. After the reaction is completed, the mixture is filtered, washed with water and dried to obtain modified montmorillonite.

[0034] The preparation process of oyster polypeptide is as follows:

[0035] According to the material-liquid ratio of 1g:3mL, water was added to the oyster meat homogenate to adjust the pH to 2; then, pepsin was added according to 0.5% of the mass of the oyster meat homogenate, and enzymatic hydrolysis was performed at 38°C for 3.2h. After the enzyme was inactivated in a boiling water bath for 10min, the supernatant was collected; after ultrafiltration of the supernatant, an oyster polypeptide with a molecular weight of less than 10kDa was obtained, which was obtained after drying.

[0036] The preparation process of aged zeolite is as follows: the zeolite is heated at 1200°C for 2 hours, cooled to room temperature, and then aged at 35°C for 9 days to obtain the zeolite.

[0037] This embodiment also provides a nutrient for improving the quality of velvet antlers, which is prepared by the above-mentioned preparation method.

[0038] Example 2

[0039] This embodiment provides a nutrient for improving the quality of velvet antlers. The nutrient is composed of the following raw materials in parts by weight: 15 parts of aged zeolite, 6 parts of modified montmorillonite, 1 part of oyster polypeptide, and 0.2 parts of vitamin E.

[0040] Among them, the preparation process of modified montmorillonite is as follows:

[0041] (1) According to the mass ratio of montmorillonite, zinc chloride and octadecyltrimethylammonium chloride of 100:4:1, montmorillonite is added to a zinc chloride solution with a concentration of 0.01 g / mL, and then octadecyltrimethylammonium chloride is added to obtain a mixed solution; the pH of the mixed solution is adjusted to 7, and the mixture is stirred at 75° C. for 3 hours. After the reaction is completed, the mixture is filtered, washed, dried, and calcined at 450° C. for 6 hours to obtain a pretreated montmorillonite;

[0042] (2) adding the pretreated montmorillonite of step (1) to a mixed solution of ethanol and water (v / v=3:1) according to the amount ratio of the pretreated montmorillonite, 3-aminopropyltriethoxysilane and the mixed solution of 1 g:0.5 g:120 mL, then adding 3-aminopropyltriethoxysilane, heating at 45° C. for 12 h, filtering, washing and drying after the reaction is completed to obtain silane coupling agent modified montmorillonite;

[0043] (3) According to the amount ratio of silane coupling agent modified montmorillonite, lauric acid, DCC, DMAP and DMF in step (2) of 10:25 g:8 g:4 g:100 mL, the silane coupling agent modified montmorillonite in step (2) is added to DMF, and then lauric acid, DCC and DMAP are added, and the mixture is heated at 75° C. for reaction for 3 h. After the reaction is completed, the mixture is filtered, washed with water and dried to obtain modified montmorillonite.

[0044] The preparation process of oyster polypeptide is as follows:

[0045] According to the material-liquid ratio of 1g:2mL, water was added to the oyster meat homogenate to adjust the pH to 1; then pepsin was added according to 0.5% of the mass of the oyster meat homogenate, and enzymatic hydrolysis was performed at 37°C for 4h. After the enzyme was inactivated in a boiling water bath for 10min, the supernatant was collected; after ultrafiltration of the supernatant, an oyster polypeptide with a molecular weight of less than 10kDa was obtained, which was obtained after drying.

[0046] The preparation process of aged zeolite is the same as that in Example 1.

[0047] This embodiment also provides a nutrient for improving the quality of velvet antlers, which is prepared by the above-mentioned preparation method.

[0048] Example 3

[0049] This embodiment provides a nutrient for improving the quality of velvet antlers. The nutrient is composed of the following raw materials in parts by weight: 20 parts of aged zeolite, 9 parts of modified montmorillonite, 5 parts of oyster polypeptide, and 0.5 parts of vitamin C.

[0050] Among them, the preparation process of modified montmorillonite is as follows:

[0051] (1) According to the mass ratio of montmorillonite, zinc chloride and octadecyltrimethylammonium chloride of 100:12:5, montmorillonite is added to a zinc chloride solution with a concentration of 0.03 g / mL, and then octadecyltrimethylammonium chloride is added to obtain a mixed solution; the pH of the mixed solution is adjusted to 7, and the mixture is stirred at 85° C. for 2 hours. After the reaction is completed, the mixture is filtered, washed, dried, and calcined at 450° C. for 6 hours to obtain a pretreated montmorillonite;

[0052] (2) adding the pretreated montmorillonite of step (1) to a mixed solution of ethanol and water (v / v=3:1) according to the amount ratio of the pretreated montmorillonite, 3-aminopropyltriethoxysilane and the mixed solution of 1 g:2 g:200 mL, then adding 3-aminopropyltriethoxysilane, heating at 55° C. for 10 h, filtering, washing and drying after the reaction is completed to obtain silane coupling agent modified montmorillonite;

[0053] (3) According to the amount ratio of silane coupling agent modified montmorillonite, lauric acid, DCC, DMAP and DMF in step (2), 10 g: 35 g: 12 g: 9 g: 100 mL, the silane coupling agent modified montmorillonite in step (2) is added to DMF, and then lauric acid, DCC and DMAP are added, and the mixture is heated at 85° C. for 2 h. After the reaction is completed, the mixture is filtered, washed with water and dried to obtain modified montmorillonite.

[0054] The preparation process of oyster polypeptide is as follows:

[0055] According to the material-liquid ratio of 1g:4mL, water was added to the oyster meat homogenate to adjust the pH to 3; then, pepsin was added according to 0.5% of the mass of the oyster meat homogenate, and enzymatic hydrolysis was performed at 40°C for 3h. After the enzyme was inactivated in a boiling water bath for 10min, the supernatant was collected; after ultrafiltration of the supernatant, an oyster polypeptide with a molecular weight of less than 10kDa was obtained, which was obtained after drying.

[0056] The preparation process of aged zeolite is the same as that in Example 1.

[0057] This embodiment also provides a nutrient for improving the quality of velvet antlers, which is prepared by the above-mentioned preparation method.

[0058] Comparative Example

[0059] Comparative Example 1

[0060] This comparative example provides a nutrient, which is composed of the following raw materials in parts by weight: 18 parts of aged zeolite, 7 parts of montmorillonite, 3 parts of oyster polypeptide, and 0.4 parts of vitamin C. The specific preparation method of the nutrient is the same as that of Example 1.

[0061] Comparative Example 2

[0062] This comparative example provides a nutrient, which is composed of the following raw materials in parts by weight: 18 parts of aged zeolite, 7 parts of a mixture of montmorillonite and lauric acid, 3 parts of oyster polypeptide, and 0.4 parts of vitamin C. The specific preparation method of the nutrient is the same as that in Example 1, and the amounts of montmorillonite and lauric acid used are consistent with those in Example 1.

[0063] Comparative Example 3

[0064] The difference between Comparative Example 3 and Example 1 is that the preparation process of the modified montmorillonite is adjusted. The details are as follows:

[0065] Step (1) was omitted, and montmorillonite was used instead of pretreated montmorillonite for subsequent preparation. The preparation conditions were the same as those in Example 1.

[0066] Comparative Example 4

[0067] This embodiment provides a nutrient, which is composed of the following raw materials in parts by weight: 18 parts of aged zeolite, 7 parts of modified montmorillonite, 3 parts of oyster polypeptide, and 0.4 parts of vitamin C, wherein the molecular weight of the oyster polypeptide is ≥10 kDa.

[0068] Test example

[0069] The properties of the nutrients obtained in Examples 1-3 and Comparative Examples 1-4 were tested as follows:

[0070] Test subjects: 40 3-year-old male sika deer with similar health, body condition and antler production were selected, 5 in each group, and randomly divided into Example 1-3 groups, Comparative Example 1-4 groups and a blank control group.

[0071] Feeding management: The Example 1-3 groups added 0.1% of the nutrients obtained in the Example 1-3 to the basic diet; the Comparative Example 1-4 groups added 0.1% of the nutrients obtained in the Comparative Example 1-4 to the basic diet; the blank control group was fed with a basic diet, which was 45% corn, 36% soybean meal, 15.7% wheat bran, 2% minerals, 1% calcium hydrogen phosphate, and 0.3% salt; feeding was performed at 7 am and 17 pm every day, and each head was fed 4kg of concentrate and 4.5kg of green feed every day during the antler growth period, and green feed was fed 1 hour after eating the concentrate. The pre-feeding period was 7 days, and the main feeding period was 2 months.

[0072] Antler yield: After the growth and maturity of the antlers meet the harvesting requirements, professional personnel at the test site anesthetize each sika deer and harvest fresh antlers. After the antlers are harvested, they are weighed and the antler base circumference (the circumference close to the middle of the horn handle of the frontal bone) and the trunk length (the natural length from the base of the antler to the tip) are accurately measured. The results are shown in Table 1.

[0073] Antler quality: The determination methods of antler dry matter, crude protein and crude fat were based on the method described in Zhang Liying (2016) Feed Analysis and Feed Quality Testing Technology 4th Edition. The results are shown in Table 1.

[0074] Table 1

[0075]

[0076] It can be seen from Table 1 that the nutrients obtained in Examples 1-3 can increase the yield of velvet antlers and improve the quality of velvet antlers.

[0077] Compared with Examples 1-3, in Comparative Example 1, modified montmorillonite is replaced by montmorillonite, in Comparative Example 2, modified montmorillonite is replaced by montmorillonite and lauric acid, in Comparative Example 3, montmorillonite is used instead of pretreated montmorillonite for subsequent preparation, and in Comparative Example 4, nutrients obtained by using oyster polypeptides with a molecular weight of ≥10kDa all lead to reduced antler yield and quality. As can be seen from the above, the nutrients obtained by the present invention include aged zeolite, modified montmorillonite, oyster polypeptides and other ingredients, and the combination of each ingredient can effectively improve the yield and quality of antlers. Among them, montmorillonite has better adsorption, dispersibility, biocompatibility and biological activity after modification. It can be used together with aged zeolite to adsorb harmful substances in the rumen and intestines of deer, has the effects of deworming and sterilization, and provides deer with trace elements necessary for growth, thereby improving the immune function of deer, improving intestinal health, promoting the absorption of nutrients in feed by deer, improving feed conversion rate, and significantly improving the yield and quality of antlers. Oyster polypeptides have multiple biological activities, such as anti-oxidation, anti-inflammatory and immunomodulatory. During the growth process of antlers, they improve the yield and quality of antlers by enhancing immunity.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.

Claims

1. A nutrient for improving the quality of velvet antler, characterized in that: The invention comprises the following raw materials in parts by weight: 15-20 parts of aged zeolite, 6-9 parts of modified montmorillonite, 1-5 parts of oyster polypeptide and 0.2-0.5 parts of vitamins; The preparation process of the modified montmorillonite is as follows: (1) adding montmorillonite to a zinc chloride solution, and then adding octadecyltrimethylammonium chloride to obtain a mixed solution; adjusting the pH of the mixed solution, and then stirring the mixed solution for reaction; after the reaction is completed, filtering, washing, drying, and calcining to obtain pretreated montmorillonite; (2) adding the pretreated montmorillonite of step (1) to a mixed solution of ethanol and water, and then adding a silane coupling agent to carry out a heating reaction, and after the reaction is completed, purifying to obtain a silane coupling agent modified montmorillonite; (3) adding the silane coupling agent modified montmorillonite in step (2) into DMF, and then adding lauric acid, DCC and DMAP to carry out heating reaction. After the reaction is completed, the modified montmorillonite is purified to obtain the modified montmorillonite.

2. The nutrient for improving the quality of velvet antler as claimed in claim 1, characterized in that: In step (3), the usage ratio of the silane coupling agent modified montmorillonite, lauric acid, DCC, DMAP and DMF in step (2) is 10 g: (25-35) g: (8-12) g: (4-9) g: 100 mL.

3. The nutrient for improving the quality of velvet antler as claimed in claim 1, characterized in that: The temperature of the heating reaction in step (3) is 75-85° C., and the heating reaction time is 2-3 h.

4. The nutrient for improving the quality of velvet antler according to claim 1, characterized in that: The mass ratio of montmorillonite, zinc chloride and octadecyltrimethylammonium chloride in step (1) is 100:(4-12):(1-5); the concentration of the zinc chloride solution is 0.01-0.03 g / mL; and the pH of the mixed solution is adjusted to 7.

5. The nutrient for improving the quality of velvet antler according to claim 1, characterized in that: In step (1), the stirring reaction temperature is 75-85°C, and the stirring reaction time is 2-3h; the calcination temperature is 450-550°C, and the calcination time is 4-6h.

6. The nutrient for improving the quality of velvet antler according to claim 1, characterized in that: In step (2), the dosage ratio of the pretreated montmorillonite, silane coupling agent and mixed solution of step (1) is 1g: (0.5-2)g: (120-200)mL; the silane coupling agent is 3-aminopropyltriethoxysilane; and the volume ratio of ethanol to water in the mixed solution of ethanol and water is 3:

1.

7. The nutrient for improving the quality of velvet antler according to claim 1, characterized in that: The temperature of the heating reaction in step (2) is 45-55° C., and the heating reaction time is 10-12 h.

8. The nutrient for improving the quality of velvet antler according to claim 1, characterized in that: The preparation process of the oyster polypeptide is as follows: According to the material-liquid ratio of 1g: (2-4)mL, water is added to the oyster meat homogenate to adjust the pH to 1-3; then pepsin is added according to 0.5% of the mass of the oyster meat homogenate, and enzymatic hydrolysis is carried out at 37-40°C for 3-4h. After the enzyme is inactivated in a boiling water bath for 10min, the supernatant is collected; after ultrafiltration of the supernatant, an oyster polypeptide with a molecular weight of less than 10kDa is obtained, which is obtained after drying.

9. The nutrient for improving the quality of velvet antler according to claim 1, characterized in that: The vitamin is vitamin C or vitamin E.

10. The method for preparing the nutrient for improving the quality of velvet antler according to any one of claims 1 to 9, characterized in that: The following steps are involved: The raw materials are mixed according to the weight ratio.

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