Three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress

Abstract

The invention discloses a three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress, which comprises a cell culture device main body, the cell culture device main body comprises a culture dish, a fixed frame and a movable frame, the movable frame is arranged on the fixed frame, the fixed frame is provided with a fixed clamp, the movable frame is provided with a movable clamp, and the movable clamp is fixed on the culture dish. A culture dish is placed on a fixing frame, cells based on collagen hydrogel embedding are cultured in the culture dish, hydrogel is fixed through a fixed clamp and a movable clamp, then a movable frame moves, and the movable clamp drives the collagen hydrogel to be away from the fixed clamp. Accurate tensile stress can be applied on the basis of cells embedded by the collagen hydrogel, the stability of the force is ensured, the strain is flexibly applied as required, the controllable range and the accuracy are greatly improved, and the maximum tensile strain can reach 200%. Quantitative drawing force can be applied to cells, operation is easy, and a culture medium is more convenient to replace.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture devices, and more particularly to a three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress. Background Art

[0002] Cells are in a three-dimensional dynamic microenvironment in the body and are constantly exposed to a variety of microenvironmental stimuli that regulate cell functions, including biochemical signals, mechanical stress fields, extracellular matrix (ECM), and cell-to-cell interactions. Its biological behavior is not only regulated by chemical signals, but also affected by complex mechanical stresses such as shear stress, compression, and stretching. In recent years, studies have found that mechanical stimulation in the cell microenvironment can affect the biological behavior of cells.

[0003] Currently, the cell mechanical stress loading devices used in biomechanical research are mostly limited to the two-dimensional plane strain of cells. Mechanical stimulation of cells is achieved by applying dynamic tensile or compressive strain to the silicone membrane. For example, the Flexell mechanical loading device uses a two-dimensional substrate deformation mode to culture cells on an elastic basement membrane. Force is applied to cause the basement membrane to deform and swell, thereby applying tensile force to the cells. It is expensive and cumbersome to operate. More importantly, the cells are in a two-dimensional culture environment, which is quite different from the three-dimensional environment of cells in the body, and the cells are subjected to uneven force.

[0004] As the research on cell culture models continues to deepen, it is found that two-dimensional cell culture models do not have the environment for cell growth in vivo, and it is difficult to simulate the interaction between cells and cells and cells and extracellular matrix, which may affect the expression of some genes and proteins of cells. In order to pursue more bionic cell culture, three-dimensional culture technology came into being, which can simulate the growth state of cells in in vivo tissues. Among them, the three-dimensional culture model based on hydrogel-embedded cells is one of the most studied models.

[0005] Hydrogel (hydrophilic polymer network) can have elasticity and stretchable deformation within a certain range, has good biocompatibility, can not only simulate the three-dimensional biological microenvironment of tissue cells, but also the mechanical load can be transmitted to the embedded cells through the deformation of the hydrogel. However, there are few studies on applying tensile stress to cells under three-dimensional culture conditions. Chinese patent application CN202110356156.3 discloses a controllable 3D stretch training bioreactor based on hydrogel, which relates to a device and method for applying controllable tensile strain to hydrogels and / or cells and / or tissues encapsulated and cultured in hydrogels. The device includes the composition, structure and mold of a three-dimensional construct based on hydrogel for three-dimensional cell culture, and a guide slider combined with a magnet for applying periodic tensile strain. The resulting device provides controllable periodic tensile strain to the hydrogel or cells and / or tissues encapsulated in the hydrogel.

[0006] However, the above technical solution uses methacrylated gelatin (GelMA) hydrogel to apply tensile stress to cells under the action of a magnetic field, which can only achieve an elongation of 10-45%, and the operation is complicated, and the stability of the force cannot be ensured during the process of increasing the force. In the existing cell stretching technology, cells are mostly in a two-dimensional culture environment, and there are problems such as unstable force value and complicated operation.

[0007] Therefore, it is necessary to propose a three-dimensional cell culture device that can quantitatively apply mechanical tensile stress to at least partially solve the problems existing in the prior art. Summary of the invention

[0008] A series of simplified concepts are introduced in the Summary of the Invention, which will be further described in detail in the Detailed Description of the Invention. The Summary of the Invention does not mean to attempt to define the key features and essential technical features of the claimed technical solution, nor does it mean to attempt to determine the scope of protection of the claimed technical solution.

[0009] In order to at least partially solve the above-mentioned problems, the present invention provides a three-dimensional cell culture device that can quantitatively apply mechanical tensile stress, including: a cell culture device body, the cell culture device body including a culture dish, a fixed rack, and a movable rack, the culture dish is arranged on the fixed rack, the movable rack is arranged on the fixed rack, the fixed rack has a fixed clamp, and the movable rack has a movable clamp.

[0010] According to the three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to an embodiment of the present invention, the fixed frame includes a fixed seat plate, a first vertical support, and a second vertical support, the first vertical support and the second vertical support are arranged on the fixed seat plate at intervals, and the fixed seat plate is also provided with a support seat, the culture dish is arranged on the support seat, the fixing clamp is arranged on the first vertical support, and the movable frame is arranged between the first vertical support and the second vertical support.

[0011] According to the three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to an embodiment of the present invention, the fixing fixture comprises a first fixing block, a first vertical fixing plate, and a first fixture block, the first fixing block is arranged on the first vertical bracket, the first vertical fixing plate is arranged on the first fixing block, the first fixture block is arranged at the bottom of the first vertical fixing plate, and the first fixture block is provided with a plurality of first fixture strips.

[0012] According to the three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to an embodiment of the present invention, the movable clamp includes a second fixed block, a second vertical fixing plate, and a second clamp block, the second fixed block is arranged on the second vertical bracket, the second vertical fixing plate is arranged on the second fixed block, the second clamp block is arranged at the bottom of the second vertical fixing plate, and the second clamp block is provided with a plurality of second clamp strips.

[0013] According to the three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to an embodiment of the present invention, two transverse guide rods are arranged between the first vertical support and the second vertical support, and the movable frame is arranged on the two transverse guide rods.

[0014] According to the three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to an embodiment of the present invention, the movable frame comprises a movable seat and a screw rod body, the movable seat is arranged on two transverse guide rods, one end of the screw rod body is rotatably connected to the movable seat, and the other end passes through the second vertical bracket and is connected to a handle body, and the handle body is rotatably arranged on the second vertical bracket.

[0015] According to an embodiment of the present invention, the three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress further includes: a distance measuring mechanism, the distance measuring mechanism including a light rod and a scale, the light rod is arranged at one end of the movable seat, the scale is arranged on the fixed seat plate, and the light rod is located above the scale.

[0016] According to an embodiment of the present invention, the three-dimensional cell culture device that can quantitatively apply mechanical tensile stress also includes: a photographing mechanism, which includes a photographing module, a first arc-shaped hoop, and a second arc-shaped hoop, one end of the first arc-shaped hoop and one end of the second arc-shaped hoop are respectively connected to the bottom of the photographing module, and the other end of the first arc-shaped hoop is connected to the other end of the second arc-shaped hoop through a locking assembly, so that the photographing mechanism can be configured on the transverse guide rod.

[0017] According to the three-dimensional cell culture device that can quantitatively apply mechanical tensile stress according to the embodiment of the present invention, the first arc-shaped hoop is provided with a first lock core cavity and a first locking cavity, the first lock core cavity is provided with a first arc-shaped lock core, and the outer wall of the first arc-shaped hoop is provided with a first lock core moving groove connected to the first lock core cavity, the first arc-shaped lock core is provided with a first driving head, the first driving head passes through the first lock core moving groove and is connected to the first arc-shaped lock core, and the first arc-shaped lock core can be connected to the locking assembly.

[0018] According to the three-dimensional cell culture device that can quantitatively apply mechanical tensile stress according to the embodiment of the present invention, the second arc-shaped hoop is configured with a second lock core cavity and a second locking cavity, the second lock core cavity is configured with a second arc-shaped lock core, and the outer wall of the second arc-shaped hoop is provided with a second lock core moving groove connected to the second lock core cavity, the second arc-shaped lock core is provided with a second drive head, the second drive head passes through the second lock core moving groove and is connected to the second arc-shaped lock core, and the second arc-shaped lock core can be connected to the locking assembly.

[0019] Compared with the prior art, the present invention has at least the following beneficial effects:

[0020] The present invention provides a three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress, the three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress comprises a cell culture device body, the cell culture device body comprises a culture dish, a fixed frame, and a mobile frame, wherein the mobile frame is mounted on the fixed frame, the fixed frame has a fixed clamp, the mobile frame has a mobile clamp, the culture dish is placed on the fixed frame, the cells embedded in collagen hydrogel are cultured in the culture dish, the fixed clamp and the mobile clamp are used to fix the collagen hydrogel, and then the mobile frame is moved so that the mobile clamp drives the collagen hydrogel and moves away from the fixed clamp. Through the design of the above structure, the present invention can apply precise tensile stress based on the cells embedded in the collagen hydrogel, ensure the stability of the force, flexibly apply strain as needed, and greatly improve the controllable range and accuracy, up to 200% tensile strain. It is also possible to apply a quantitative tensile force to the cells, the operation is simple, and it is more convenient to replace the culture medium. The device can be reused many times.

[0021] The three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress described in the present invention, other advantages, objectives and features of the present invention will be partially reflected through the following description, and will also be partially understood by technicians in this field through research and practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0023] Figure 1 It is a structural schematic diagram of the present invention.

[0024] Figure 2 A partial structural top view of the present invention Figure 1 .

[0025] Figure 3 It is a schematic diagram of the structure of the fixing fixture in the present invention.

[0026] Figure 4 A partial structural top view of the present invention Figure 2 .

[0027] Figure 5 It is a structural schematic diagram of the mobile rack in the present invention.

[0028] Figure 6 It is a structural schematic diagram of the photographing mechanism in the present invention.

[0029] Figure 7 It is a schematic diagram of a partial explosion structure of the photographing mechanism in the present invention.

[0030] Figure 8 It is a schematic structural diagram of the first arc-shaped hoop in the present invention.

[0031] Fig. 9 It is a schematic structural diagram of the second arc-shaped hoop in the present invention.

[0032] Fig.10 It is a schematic diagram of the structure of the locking component in the present invention.

[0033] Fig.11 The structure of the first locking member in the present invention is schematically shown in FIG. Figure 1 .

[0034] Fig.12 The structure of the first locking member in the present invention is schematically shown in FIG. Figure 2 . DETAILED DESCRIPTION

[0035] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments so that those skilled in the art can implement the invention with reference to the description.

[0036] It should be understood that the terms such as “having”, “including” and “comprising” used herein do not exclude the existence or addition of one or more other elements or combinations thereof.

[0037] like Figure 1-Figure 5As shown, the present invention provides a three-dimensional cell culture device that can quantitatively apply mechanical tensile stress, including: a cell culture device body 100, the cell culture device body 100 includes a culture dish 1, a fixed frame 2, and a mobile frame 3, wherein the mobile frame 3 is installed on the fixed frame 2, the fixed frame 2 has a fixed clamp 20, and the mobile frame 3 has a mobile clamp 30, the culture dish 1 is placed on the fixed frame 2, and cells embedded in collagen hydrogel are cultured in the culture dish 1, and the fixed clamp 20 and the mobile clamp 30 are used to fix the collagen hydrogel, and then the mobile frame 3 is moved, so that the mobile clamp 30 drives the collagen hydrogel and moves away from the fixed clamp 20. Through the design of the above structure, the present invention can apply precise tensile stress based on cells embedded in collagen hydrogel, ensure the stability of force, flexibly apply strain as needed, and greatly improve the controllable range and accuracy, up to 200% tensile strain. It is also possible to apply quantitative tensile force to cells, which is simple to operate and more convenient to replace the culture medium. The device can be reused many times.

[0038] Exemplary Mounting Bracket

[0039] Further, some embodiments of the present invention provide a specific structure of the above-mentioned fixed frame 2, wherein the fixed frame 2 of the structure includes a fixed seat plate 21, a first vertical bracket 22, and a second vertical bracket 23, wherein the first vertical bracket 22 and the second vertical bracket 23 are installed on the fixed seat plate 21 at intervals, and the mobile frame 3 is installed between the first vertical bracket 22 and the second vertical bracket 23, and there is enough space for the mobile frame 3 to move between the first vertical bracket 22 and the second vertical bracket 23, and a support seat 211 is also installed on the fixed seat plate 21, and the culture dish 1 can be fixed by the support seat 211, so that it is more convenient to replace the culture medium and it can be reused many times;

[0040] The above-mentioned fixed fixture 20 is installed on the first vertical support 22, so by moving the movable frame 3, the movable fixture 30 can be moved away from the fixed fixture 20, thereby achieving the stretching of the cells embedded in the collagen hydrogel.

[0041] Furthermore, two transverse guide rods 24 are installed between the first vertical support 22 and the second vertical support 23, and the mobile rack 3 is installed on the two transverse guide rods 24. The two transverse guide rods 24 provide fixed guidance for the movement of the mobile rack 3, so that the mobile rack 3 is more stable when stretching cells.

[0042] It can be understood that the first vertical bracket 22 and the second vertical bracket 23 can adopt the same structure. Here, the first vertical bracket 22 is taken as an example for explanation. The first vertical bracket 22 includes two vertical support rods 221 and a bracket seat 222. The bracket seat 222 is installed on the two vertical support rods 221. Two fixing holes are opened on the bracket seat 222, and the ends of the transverse guide rod 24 can be installed in the fixing holes.

[0043] Exemplary Fixture

[0044] Furthermore, some embodiments of the present invention provide a specific structure of the above-mentioned fixing fixture 20, wherein the fixing fixture 20 of this structure includes a first fixing block 201, a first vertical fixing plate 202, and a first fixture block 203, wherein the first fixing block 201 is installed on the first vertical bracket 22, the first vertical fixing plate 202 is installed on the first fixing block 201, the first fixture block 203 is installed at the bottom of the first vertical fixing plate 202 and faces the movable frame 3, and a plurality of first fixture strips 204 are installed on the first fixture block 203, for example, two first fixture strips 204 can be installed, so as to fix two collagen hydrogel-embedded cells and improve work efficiency.

[0045] Exemplary Mobile Fixture

[0046] Further, some embodiments of the present invention provide a specific structure of the mobile fixture 30, wherein the mobile fixture 30 of the structure includes a second fixed block 301, a second vertical fixing plate 302, and a second fixture block 303. Specifically, the second fixed block 301 is mounted on the second vertical bracket 23, and the second vertical fixing plate 302 is mounted on the second fixed block 301. The second fixture block 303 is mounted on the bottom of the second vertical fixing plate 302. The second fixture block 303 faces the fixed fixture 20, and a plurality of second fixture strips 304 are mounted on the second fixture block 303. For example, two second fixture strips 304 can be installed, opposite to the first fixture strips 204, so that two collagen hydrogel-based cells can be fixed to improve work efficiency.

[0047] Exemplary mobile rack

[0048] Furthermore, some embodiments of the present invention provide a specific structure of the above-mentioned moving frame 3, where the moving frame 3 of the structure includes a moving seat 31 and a screw rod body 32, wherein the moving seat 31 is mounted on two transverse guide rods 24, and one end of the screw rod body 32 is rotatably connected to the moving seat 31, and the other end passes through the second vertical bracket 23 and is connected to the handle body 33, and the handle body 33 is rotatably mounted on the second vertical bracket 23, so by rotating the handle body 33, the screw rod 32 can be moved in the handle body 33, and then the moving seat 31 is driven to move along the two transverse guide rods 24, and the moving fixture 30 is driven to apply precise tensile stress to the cells embedded in the collagen hydrogel, thereby ensuring the stability of the force. For example, one rotation of the handle body 33 is 1mm, and the handle body 33 is also marked with a scale with an accuracy of 0.1mm.

[0049] Furthermore, it also includes: a distance measuring mechanism, which includes a light rod 34 and a scale 35. The light rod 34 is installed at one end of the movable seat 31, and the scale 35 is installed on the fixed seat plate 21. The light rod 34 is located above the scale 35, so after the handle body 33 is turned, the light rod 34 moves along the scale 35, so that strain can be flexibly applied as needed, and the controllable range and accuracy are greatly improved, up to 200% tensile strain, and a quantifiable tensile force can be applied to the cells, and the operation is simple.

[0050] Generally speaking, the length of the stretching part is 25 mm, and the maximum stretching length is 50 mm. When using a culture dish 1, the maximum stretching size of a culture dish 1 with a diameter of 85 mm is 55.46 mm (the stretching part in the middle). The maximum stretching size of a culture dish 1 with a diameter of 100 mm is 70.46 mm (the stretching part in the middle).

[0051] Exemplary photography mechanism

[0052] like Figure 6-Figure 12 As shown, further, some embodiments of the present invention provide a photographing mechanism 4, where the photographing mechanism 4 is installed on one of the transverse guide rods 24, and the photographing mechanism 4 is used to photograph the collagen hydrogel during the stretching process to obtain the shape changes of the cells at different stretching lengths, so that different stretching lengths are matched with the cell shape, making the research results more physiologically meaningful, and can be used to carry out research such as exploring the impact of stretching stress on cell behavior.

[0053] like Figure 6-Figure 7 As shown, specifically, the camera mechanism 4 of the structure includes a camera module 41, a first arc hoop 42, and a second arc hoop 43, wherein one end of the first arc hoop 42 and one end of the second arc hoop 43 are respectively connected to the bottom of the camera module 41, and the other end of the first arc hoop 42 is connected to the other end of the second arc hoop 43 through a locking assembly 5, so that the camera mechanism 4 can be installed on the transverse guide rod 24, and the locking assembly 5 also facilitates the subsequent separation of the first arc hoop 42 and the second arc hoop 43 from each other to accommodate the camera module 41.

[0054] Exemplary first arc-shaped hoop

[0055] like Figure 8As shown, further, some embodiments of the present invention provide a specific structure of the first arc-shaped hoop 42, wherein the first arc-shaped hoop 42 of the structure is provided with a first lock core cavity 421 and a first locking cavity 422, a first arc-shaped lock core 423 is installed in the first lock core cavity 421, and a first lock core moving groove 424 communicating with the first lock core cavity 421 is provided on the outer wall of the first arc-shaped hoop 42, a first drive head 425 is provided on the first arc-shaped lock core 423, and the first drive head 425 passes through the first lock core moving groove 424 and is connected to the first arc-shaped lock core 423, so the first arc-shaped lock core 423 can be driven by the first drive head 425 to move outward from the first lock core moving groove 424 to the second arc-shaped hoop 43, and fixed by the locking assembly 5;

[0056] Exemplary Second Arc Hoop

[0057] like Fig. 9 As shown, further, some embodiments of the present invention provide a specific structure of the second arc-shaped hoop 43, wherein the second arc-shaped hoop 43 of the structure is provided with a second lock core cavity 431 and a second locking cavity 432, a second arc-shaped lock core 433 is installed in the second lock core cavity 431, and a second lock core moving groove 434 communicating with the second lock core cavity 431 is provided on the outer wall of the second arc-shaped hoop 43, a second drive head 435 is provided on the second arc-shaped lock core 433, the second drive head 435 passes through the second lock core moving groove 434 and is connected to the second arc-shaped lock core 433, and the second arc-shaped lock core 433 can be connected to the locking assembly 5. Therefore, the second arc-shaped lock core 433 can be driven by the second drive head 435 to move outward from the second lock core cavity 431 to the first arc-shaped hoop 42, and is fixed by the locking assembly 5, so as to realize the locking of the first arc-shaped hoop 42 and the second arc-shaped hoop 43 by the locking assembly 5, and the process is simple to operate and convenient to use.

[0058] Exemplary Locking Assemblies

[0059] like Figure 10-12 As shown, further, some embodiments of the present invention provide a specific structure of the above-mentioned locking assembly 5, where the locking assembly 5 of the structure includes a first locking piece 6 and a second locking piece 7. It can be understood that the structures of the first locking piece 6 and the second locking piece 7 are the same, and the two are symmetrically installed at the other end of the first arc hoop 42 and the other end of the second arc hoop 43, and then the first locking piece 6 can be used to lock the above-mentioned first arc lock core 423 to fix the second arc hoop 43, and the second locking piece 7 can be used to lock the above-mentioned second arc lock core 433 to fix the first arc hoop 42, so as to achieve mutual firm locking between the first arc hoop 42 and the second arc hoop 43.

[0060] Exemplary first locking member

[0061] Further, some embodiments of the present invention provide a specific structure of the above-mentioned first locking member 6, wherein the first locking member 6 of the structure includes a locking head 61, on which are two locking supports 62, and between which are swinging spaces 63, an axis groove 621 is installed on the inner side of the locking support 62, a first inner shaft body 622 is installed in the axis groove 621, a first swing plate 623 is installed on the first inner shaft body 622, and tension spring bodies 624 are respectively arranged on both sides of the first swing plate 623, further, a second swing plate 625 is also installed at the end of the first inner shaft body 622, an inner locking card plate 611 is also installed on the locking head 61, and the second swing plate 625 is movably connected to the inner locking card plate 611;

[0062] When the second arc lock core 433 is moved out of the second lock core cavity 431, it enters the first locking cavity 422 of the first arc hoop 42. At this time, the operator can swing the two first swing plates 623, and then the two first swing plates 623 swing in the swing space 63 toward the locking head 61, thereby driving the second swing plate 625 to swing as well, and then the two inner locking cards 611 located in the first locking cavity 422 are moved away from each other, and the end of the second arc lock core 433 is further moved between the two inner locking cards 611. After the operator releases the first swing plate 623, the first swing plate 623 is pulled back to the original position under the action of the tension spring body 624, so that the two inner locking cards 611 are close to each other and lock the end of the second arc lock core 433. Similarly, by swinging the two first swing plates 623 again, the locking of the second arc lock core 433 by the two inner locking cards 611 can be easily unlocked. This process is simple to operate and convenient to use.

[0063] Further, a second inner shaft 612 is mounted on the locking head 61, a third swing plate 613 is mounted on one end of the second inner shaft 612, and the inner locking card plate 611 is mounted on the other end of the second inner shaft 612, and the inner locking card plate 611 has a plurality of convex teeth 614, a third inner shaft 626 is also mounted on the locking support 62, a V-shaped torsion spring 627 is mounted on the end of the third inner shaft 626, and a first torsion spring rod 6271 of the V-shaped torsion spring 627 is connected to the second swing plate 625 via a first pull rod 628, and a second torsion spring rod 6272 of the V-shaped torsion spring 627 is connected to the third swing plate 613 via a second pull rod 629;

[0064] Therefore, when the second swing plate 625 swings, the first pull rod 628 actuates the V-shaped torsion spring 627, so that the V-shaped torsion spring 627 swings on the third inner shaft 626, and then the second torsion spring rod 6272 drives the third swing plate 613 to swing through the second pull rod 629, and then the third swing plate 613 drives the inner locking card plate 611 to actuate through the second inner shaft 612, so that the two inner locking card plates 611 lock or unlock the second arc-shaped lock core 433;

[0065] Among them, there are multiple convex teeth 614 on the inner locking card plate 611, and the second arc-shaped lock core 433 has a locking groove corresponding to the convex teeth 614, which prevents the second arc-shaped lock core 433 from separating from the inner locking card plate 611, greatly improving the locking effect. The process is simple to operate and easy to use.

[0066] In the description of the present invention, it is to be understood that the terms “center”, “longitudinal”, “lateral”, “length”, “width”, “thickness”, “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inside”, “outside”, “clockwise”, “counterclockwise”, “axial”, “radial”, “circumferential”, etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0067] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection, an electrical connection, or communication with each other; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0068] Although the embodiments of the present invention have been disclosed as above, they are not limited to the applications listed in the specification and the implementation modes, and they can be fully applied to various fields suitable for the present invention. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the present invention is not limited to the specific details and the illustrations shown and described herein.

Claims

1. A three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress, characterized in that: include: A cell culture device body (100) comprises a culture dish (1), a fixed frame (2), and a movable frame (3); the culture dish (1) is arranged on the fixed frame (2); the movable frame (3) is arranged on the fixed frame (2); the fixed frame (2) has a fixed fixture (20); and the movable frame (3) has a movable fixture (30).

2. A three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to claim 1, characterized in that: The fixed frame (2) comprises a fixed seat plate (21), a first vertical support (22), and a second vertical support (23); the first vertical support (22) and the second vertical support (23) are arranged on the fixed seat plate (21) at intervals, and the fixed seat plate (21) is also provided with a support seat (211); the culture dish (1) is arranged on the support seat (211); the fixed clamp (20) is arranged on the first vertical support (22); and the movable frame (3) is arranged between the first vertical support (22) and the second vertical support (23).

3. A three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to claim 1, characterized in that: The fixing fixture (20) comprises a first fixing block (201), a first vertical fixing plate (202), and a first fixture block (203); the first fixing block (201) is arranged on the first vertical bracket (22); the first vertical fixing plate (202) is arranged on the first fixing block (201); the first fixture block (203) is arranged at the bottom of the first vertical fixing plate (202); and the first fixture block (203) is provided with a plurality of first fixture strips (204).

4. The three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to claim 1, characterized in that: The movable clamp (30) comprises a second fixed block (301), a second vertical fixing plate (302), and a second clamp block (303); the second fixed block (301) is arranged on the second vertical bracket (23); the second vertical fixing plate (302) is arranged on the second fixed block (301); the second clamp block (303) is arranged at the bottom of the second vertical fixing plate (302); and the second clamp block (303) is provided with a plurality of second clamp strips (304).

5. The three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to claim 2, characterized in that: Two transverse guide rods (24) are arranged between the first vertical support (22) and the second vertical support (23), and the movable frame (3) is arranged on the two transverse guide rods (24).

6. The three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to claim 2, characterized in that: The movable frame (3) comprises a movable seat (31) and a screw rod body (32); the movable seat (31) is arranged on two transverse guide rods (24); one end of the screw rod body (32) is rotatably connected to the movable seat (31); the other end passes through the second vertical bracket (23) and is connected to a handle body (33); the handle body (33) is rotatably arranged on the second vertical bracket (23).

7. The three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to claim 6, characterized in that: Also includes: The distance measuring mechanism comprises a polished rod (34) and a scale (35), wherein the polished rod (34) is arranged at one end of a movable seat (31), the scale (35) is arranged on a fixed seat plate (21), and the polished rod (34) is located above the scale (35).

8. The three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to claim 6, characterized in that: Also includes: A photographing mechanism (4), the photographing mechanism (4) comprising a photographing module (41), a first arc-shaped hoop (42), and a second arc-shaped hoop (43), one end of the first arc-shaped hoop (42) and one end of the second arc-shaped hoop (43) are respectively connected to the bottom of the photographing module (41), and the other end of the first arc-shaped hoop (42) is connected to the other end of the second arc-shaped hoop (43) through a locking assembly (5), so that the photographing mechanism (4) can be arranged on the transverse guide rod (24).

9. The three-dimensional cell culture device capable of quantitatively applying mechanical tensile stress according to claim 8, characterized in that: The first arc-shaped hoop (42) is provided with a first lock core cavity (421) and a first locking cavity (422); the first lock core cavity (421) is provided with a first arc-shaped lock core (423); and the outer wall of the first arc-shaped hoop (42) is provided with a first lock core movable groove (424) which is connected with the first lock core cavity (421); the first arc-shaped lock core (423) is provided with a first driving head (425); the first driving head (425) passes through the first lock core movable groove (424) and is connected with the first arc-shaped lock core (423); the first arc-shaped lock core (423) can be connected with the locking assembly (5).

Images