Method for detecting medetomidate hydrochloride and analogues thereof by using metalloporphyrin

Abstract

The invention provides a rapid and cheap method for detecting medetomidate hydrochloride and analogues thereof by using metalloporphyrin, and aims to solve the problems of high cost, tedious operation and the like caused by dependence on complex equipment in the prior art. The method is based on the characteristic that metalloporphyrin and imidazole ring nitrogen atoms in medetomidate hydrochloride and analogues thereof are subjected to coordination reaction, and after a complex is formed, the color of a solution is changed from reddish brown to yellow green. In addition, an ultraviolet-visible spectrophotometer can be used for further verifying the red shift phenomenon of a Sore absorption peak in an absorbance curve. In specific implementation, a sample to be detected is dissolved in anhydrous dichloromethane, a metalloporphyrin solution is added and mixed, and qualitative analysis is realized by visually observing color change or detecting absorbance change by means of an instrument. Preferably, the metalloporphyrin comprises hemin, 5, 10, 15, 20-tetraphenylporphyrin iron (III) chloride and the like, and the substances to be detected contain analogues such as medetomidate hydrochloride, etomidate hydrochloride and the like. Experimental results show that the method has high sensitivity and reliability, is suitable for rapidly screening medetomidate hydrochloride-containing cigarettes and other samples, and provides a convenient detection means for law enforcement departments.

Description

Technical Field

[0001] The invention relates to the field of molecular sensors, and in particular to a method for detecting medetomidine and its analogs by using metalloporphyrin. Background Art

[0002] The present invention relates to the field of psychotropic drug detection technology, and specifically to a qualitative detection method for medetomide hydrochloride and its analogs. Medetomide is commonly used as a veterinary anesthetic, and etomidate has been listed as a controlled psychotropic drug since October 2023. Because medetomide is similar in structure to etomidate, medetomide hydrochloride is illegally added to cigarette tobacco and electronic cigarette oil as a substitute for etomidate hydrochloride. Current detection mainly relies on gas chromatography and liquid chromatography techniques. Although accurate quantitative analysis can be achieved, there are technical defects such as the need for complex sample pretreatment, reliance on professionals to analyze spectra, and the need to configure large-scale instruments and equipment such as high-precision chromatographic separation devices and mass spectrometers, resulting in the existing methods being expensive, cumbersome processes, and long detection times. There is an urgent need to develop new rapid detection solutions to solve the above-mentioned technical bottlenecks. Summary of the invention

[0003] In view of the defects of the existing detection technology such as high price and complicated process, the present invention develops a cheap and convenient method for qualitative detection of medetomidine hydrochloride and its analogs.

[0004] The technical solution adopted by the present invention to solve the above technical problems is as follows:

[0005] A method for qualitatively detecting medetomidine hydrochloride and its analogs, comprising:

[0006] Take the sample to be tested:

[0007] The sample to be tested is dissolved in anhydrous dichloromethane, and the metal porphyrin is dissolved in anhydrous dichloromethane, and the two are mixed to obtain a mixture solution.

[0008] The metal porphyrin itself is reddish brown. After adding the sample solution, the nitrogen atom on the imidazole ring of medetomidine and its analogs coordinates with the central metal atom of the metal porphyrin to form a complex. The complex exists in the above-mentioned mixture solution, causing the color of the metal porphyrin solution to change from reddish brown to yellow-green. The ultraviolet absorption spectrum shows a red shift of the porphyrin Soret absorption peak.

[0009] The above two changes can be observed by naked eyes and measured by UV-visible spectrophotometer respectively.

[0010] The following are preferred technical solutions of the present invention, but are not intended to limit the technical solutions provided by the present invention. Through the following preferred technical solutions, the objectives and beneficial effects of the present invention can be better achieved and realized.

[0011] As a preferred technical solution, the metal porphyrin includes: hemin, 5,10,15,20-tetraphenylporphyrin iron (III) chloride, 5,10,15,20-tetrakis (p-carboxyphenyl) porphyrin iron (III) chloride, and 5,10,15,20-tetrapyridyl porphyrin iron (III) chloride.

[0012] As a preferred technical solution, the medetomidine hydrochloride and its analogs to be detected include: medetomidine hydrochloride, etomidate hydrochloride, propamate hydrochloride, and ipropamate hydrochloride. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 This is a reference diagram for qualitatively detecting medetomidine hydrochloride powder by the visual detection method in Example 1 of the present invention.

[0014] Figure 2 This is a reference diagram for qualitatively detecting tobacco containing medetomidine hydrochloride by the visual detection method in Example 2 of the present invention.

[0015] Figure 3 This is the absorbance curve of qualitative detection of trace medetomidine hydrochloride by means of UV-visible spectrophotometer in Example 3 of the present invention.

[0016] Figure 4 The chemical structure of the complex formed by the combination of medetomidine hydrochloride and its analogs with metal porphyrin in the present invention. DETAILED DESCRIPTION

[0017] The present invention provides a method for detecting medetomidine hydrochloride and its analogs. In order to make the purpose, technical scheme and effect of the present invention clearer and more specific, the present invention is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0018] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various implementation methods. At the same time, the steps or actions in the method description can also be interchanged or adjusted in a manner that is obvious to those skilled in the art. Therefore, the various sequences in the specification and the drawings are only for the purpose of clearly describing a certain embodiment and do not mean that a necessary sequence is required, unless otherwise specified that a certain sequence must be followed. The serial numbers themselves, such as "first", "second", etc., assigned to the components herein are only used to distinguish the objects described and do not have any order or technical meaning. In addition, it should be understood that after reading the contents taught by the present invention, those skilled in the art may make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the claims attached to this application.

[0019] The instruments and reagents used in this example are all commercially available products.

[0020] The following is a further explanation and description of a method for detecting medetomidine hydrochloride and its analogs provided by the present invention through specific examples.

[0021] Example 1

[0022] Qualitative detection of medetomidine hydrochloride powder by visual inspection

[0023] Weigh 0.0035 g of 5,10,15,20-tetraphenylporphyrin iron (III) chloride and dissolve it in 250 ml of dichloromethane to make a solution and shake it well. Use a rubber-tipped dropper to take about 3 ml of the above solution and add it to the cuvette. Observe that the solution now appears as follows: Figure 1 The color is reddish brown as shown in the cuvette on the left. Soak a cotton swab in dichloromethane, pick up the solid medetomidine hydrochloride, immerse it in the cuvette and rotate it to dissolve the medetomidine hydrochloride in the above solution. Let it stand for 5 to 30 seconds and observe. The solution will turn into Figure 1 The yellow-green color shown in the cuvette on the right confirms that the powder contains medetomidine hydrochloride.

[0024] Example 2

[0025] Qualitative detection of medetomidine hydrochloride in cigarette tobacco by visual inspection

[0026] Take a cigarette and remove all the tobacco from it; take another cigarette of the same type and remove all the tobacco from it. Dissolve 100 mg of medetomidine hydrochloride in 3 ml of deionized water, immerse the tobacco, and dry it at 60 degrees Celsius to obtain tobacco containing medetomidine hydrochloride. Place the two tobaccos in 50 ml beakers, add 5 ml of dichloromethane to each, shake for 3 minutes, and use a syringe with a filter to extract solution 1 without medetomidine hydrochloride and solution 2 containing medetomidine hydrochloride. At this time, both may appear light brown because they contain tar. Weigh 0.0035 g of 5,10,15,20-tetraphenylporphyrin iron (III) chloride and dissolve it in 250 ml of dichloromethane to prepare solution 3 and shake well. Use a rubber-tipped dropper to take about 2 ml of the above solution 3, add it to two cuvettes, use a rubber-tipped dropper to drop about 1 ml of the above solution 1 and solution 2 into the cuvettes, and shake gently for 30 seconds. At this time, the mixture of solution 1 and 3 appears reddish brown. Figure 2 As shown in the cuvette on the left, solutions 2 and 3 appear yellow-green after mixing. Figure 2 As shown in the cuvette on the right, this phenomenon can confirm that Solution 2 contains medetomidine hydrochloride.

[0027] Example 3

[0028] Qualitative Detection of Trace Medetomidine Hydrochloride by UV-Vis Spectrophotometer

[0029] Medetomidine hydrochloride was prepared into a dichloromethane solution with a concentration of 10 μM, which was recorded as solution 1; 5,10,15,20-tetraphenylporphyrin iron (III) chloride was prepared into a dichloromethane solution with a concentration of 1000 μM, which was recorded as solution 2. Use a pipette to take 50 μL of solution 2, mix it thoroughly with 10 mL of solution 1, let it stand for 30 seconds, and then transfer it to a cuvette. Take another 10 ml of solution 1, add 50 μL of dichloromethane, shake well, and place it in a cuvette. Place the two cuvettes in a UV-visible spectrophotometer, scan the absorbance of the two at 350 nm to 500 nm, and draw an absorbance curve, the image of which is shown as follows. Figure 3 As shown. The solution containing medetomidine hydrochloride will produce the following results compared with the solution without medetomidine hydrochloride. Figure 3 The absorption peak shown is red-shifted.

Claims

1. A complex formed by a metalloporphyrin and medetomidine hydrochloride and its analogs, characterized in that: The imidazole nitrogen atom of medetomidine hydrochloride and its analogs coordinates with the central metal atom of porphyrin, and its chemical structure is as follows:

2. A complex formed by the metalloporphyrin and medetomidine hydrochloride and its analogs as claimed in claim 1, characterized in that: The metal porphyrins include: hemin, 5,10,15,20-tetraphenylporphyrin iron (III) chloride, 5,10,15,20-tetrakis (p-carboxylphenyl) porphyrin iron (III) chloride, and 5,10,15,20-tetrapyridyl porphyrin iron (III) chloride.

3. A complex formed by the metalloporphyrin as claimed in claim 1 and medetomidine hydrochloride and its analogs, characterized in that: The medetomidine hydrochloride and its analogs include medetomidine hydrochloride, etomidate hydrochloride, propamate hydrochloride and ipropamate hydrochloride.

4. A method for detecting the complex according to claim 1 using an ultraviolet-visible spectrophotometer, characterized in that: The Soret absorption peak of the complex is red-shifted compared to the original metal porphyrin, and the peak width becomes larger.

5. A method for detecting medetomidine hydrochloride and its analogs using metalloporphyrins, characterized in that: When medetomidine and its analogs are combined with metal porphyrins to form the complex as described in claim 1, they produce color visible to the naked eye and change the spectral characteristics as described in claim 4.

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