Sample detection all-in-one machine and detection method

Abstract

The invention provides a sample detection all-in-one machine. The sample detection all-in-one machine comprises a sample loading module, a consumable loading module, a reagent loading module and a material moving module, the sample loading module comprises a sample conveying line, a sample picking mechanism and a sample fixing mechanism, the consumable loading module comprises a consumable bin and a material shifting mechanism, the reagent loading module comprises a reagent adding pipe and a reagent storage container, and the reagent adding pipe is connected with the reagent storage container through a liquid conveying part. The sample loading module can be matched with assembly line conveying and automatically complete sample tube picking and sample transferring, the consumable loading module can automatically complete sequential feeding of consumables, and the reagent loading module can automatically complete adding of various reaction reagents. And the consumables and the reagents do not need to be manually supplemented in the assembly line type extraction and detection process, so that the whole equipment is adaptive to an automatic assembly line, and the extraction and detection efficiency of large-batch samples is effectively improved. The invention also provides a sample detection method.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro diagnosis, and in particular to an all-in-one sample detection machine and a detection method. Background Art

[0002] Nucleic acid testing is a type of sample testing. Nucleic acid is a biological macromolecular compound composed of many nucleotides and is one of the most basic substances of life. Nucleic acids are widely present in all animal and plant cells and microorganisms. Nucleic acids in organisms often combine with proteins to form nucleoproteins. With the popularization of genetic testing, personalized drug delivery, prenatal diagnosis, etc., the limitations of traditional DNA extraction methods are becoming more and more obvious as all fields of the biological industry pursue high throughput and automation. Since the magnetic bead method for extracting nucleic acids can achieve automated extraction, large-scale operations, and is simple to operate and takes a short time, the magnetic bead method for extracting nucleic acids has received more and more attention.

[0003] The magnetic bead method usually includes upper extraction method and lower extraction method. The upper extraction method generally uses magnetic rod and magnetic bead separation technology to extract nucleic acid and finally obtain purified nucleic acid.

[0004] In the existing solutions, most equipment requires manual loading of consumables and reagents. After completing a batch, personnel need to repeatedly add consumables and reagents. Therefore, manpower is still required and it is impossible to achieve complete intelligence and automation. Therefore, the detection efficiency is low and it is difficult to adapt to large-scale detection. The detection method using a liberalized assembly line can significantly improve the detection efficiency. If the sample detection and extraction machine wants to be connected to the automated assembly line, it must add storage locations for consumables and reagents to truly achieve unattended operation. Summary of the invention

[0005] The purpose of the present invention is to provide a sample detection solution suitable for an automated assembly line that can realize automatic loading of samples, consumables and reagents in view of the deficiencies in the above-mentioned background technology.

[0006] In order to achieve the above-mentioned object, the present invention provides a sample detection integrated machine, including a sample loading module, a consumables loading module, a reagent loading module, and a material transfer module;

[0007] The sample loading module includes a sample conveying line, a sample picking mechanism and a sample fixing mechanism; the sample conveying line corresponds to the assembly line and is used to convey sample tubes containing samples, the sample picking mechanism is used to pick up the sample tubes on the sample conveying line and transfer them to the sample fixing mechanism, the sample fixing mechanism is used to fix the sample tubes, and the material transfer module can transfer the samples in the sample tubes to consumables;

[0008] The consumable loading module includes a consumable bin and a material shifting mechanism, the consumable bin is filled with the consumables, the material shifting mechanism is used to shift the consumables in the consumable bin to the discharge port of the consumable bin in sequence, and the material shifting module can shift the consumables at the discharge port;

[0009] The reagent loading module includes a reagent addition tube and a reagent storage container. The reagent addition tube is connected to the reagent storage container through an infusion part. The infusion part is used to transport the reaction reagent in the reagent storage container to the reagent addition tube. The reagent addition tube is used to introduce the reaction reagent into the consumable.

[0010] Furthermore, the sample picking mechanism includes a sample picking claw, a first finger cylinder, a rotating shaft, a rotating shaft seat, and a picking drive unit. The sample picking claw is arranged at the output end of the first finger cylinder, and at least one pair is arranged. The first finger cylinder is fixedly connected to the rotating shaft, and the rotating shaft is rotatably connected to the rotating shaft seat. The picking drive unit is used to drive the rotating shaft to rotate and lift.

[0011] Furthermore, the picking drive unit includes a base, on which a first motor is arranged, the first motor is transmission connected to the rotating shaft through a first synchronous belt and a first synchronous wheel, and the base is also provided with a lifting guide rail, a second motor, a second synchronous wheel and a second synchronous belt, the rotating shaft seat is slidingly connected to the lifting guide rail, the second motor is transmission connected to the second synchronous wheel, the second synchronous belt is wound around the second synchronous wheel, and the second synchronous belt is fixedly connected to the rotating shaft seat.

[0012] Furthermore, the sample fixing mechanism includes a sample clamp, a sample clamp arm, a second finger cylinder and a placement seat, the sample clamp is arranged at the first end of the sample clamp arm, the second end of the sample clamp arm is connected to the output end of the second finger cylinder, the sample clamp and the sample clamp arm are each arranged in a pair, and the placement seat is located between the sample clamps.

[0013] Furthermore, the consumables bin is provided with a material tapping slot, and the material tapping mechanism includes a material tapping plate, a material tapping mounting seat fixedly connected to the material tapping plate, and a material tapping drive unit that drives the material tapping mounting seat and the material tapping plate to tap materials toward the material outlet. The material tapping plate matches the size of the material tapping slot, enters from the material tapping slot and moves toward the material outlet to tap materials.

[0014] Furthermore, the infusion part is a peristaltic pump, and the peristaltic pump and the reagent adding tube are arranged in a one-to-many correspondence, and each of the peristaltic pumps delivers a corresponding reaction reagent;

[0015] Or, the peristaltic pumps and the reagent adding tubes are arranged in a one-to-one correspondence, and each group of the peristaltic pumps correspondingly delivers one reaction reagent;

[0016] Each of the peristaltic pumps is independently controlled.

[0017] Furthermore, the sample detection integrated machine also includes a reaction carrying module, and the reaction carrying module includes a carrying platform and a carrying platform driving mechanism, the carrying platform is used to place consumables, and the carrying platform driving mechanism is used to drive the carrying platform to move.

[0018] Further, the sample extraction module includes a magnetic rod sleeve assembly and a magnetic rod assembly;

[0019] The magnetic rod sleeve assembly includes a first driving mechanism, a first mounting frame connected to the first driving mechanism, and a magnetic rod sleeve mounted on the first mounting frame; the magnetic rod assembly includes a second driving mechanism, a second mounting frame connected to the second driving mechanism, and a magnetic rod mounted on the second mounting frame, the magnetic rod is located directly above the magnetic rod sleeve, and the magnetic rod and the magnetic rod sleeve are arranged correspondingly;

[0020] The sample extraction module further includes a third driving mechanism and a driving seat. The third driving mechanism is connected to the driving seat. The first driving mechanism and the second driving mechanism are both arranged on the driving seat.

[0021] Further, the material transfer module includes a transport manipulator, a liquid transfer mechanism, a cover opening manipulator and a material transfer drive mechanism, wherein the transport manipulator, the liquid transfer mechanism and the cover opening manipulator are all connected to the material transfer drive mechanism, the transport manipulator is used to transport the consumables, the liquid transfer mechanism is used to transfer the solution, and the cover opening manipulator is used to open the tube cover of the sample tube;

[0022] The sample detection integrated machine also includes a gun tip retrieval rack and a gun tip discarding rack. The gun tip retrieval rack is used to place new gun tips, and the gun tip discarding rack is used to place discarded gun tips. The liquid transfer mechanism transfers liquid through the gun tips.

[0023] The present invention also provides a sample detection method, using the aforementioned sample detection integrated machine, comprising:

[0024] The consumables are pre-loaded in the consumables bin, and the reagents are pre-loaded in the reagent barrel. The consumables are loaded through the consumables bin, and the transport robot transfers them to the carrying table of the reaction carrying module, and the carrying table is in the first position;

[0025] The sample picking mechanism picks up each sample tube from the sample conveying line and fixes it to the sample fixing mechanism. The cover opening robot opens the tube cover of the sample tube. The pipetting mechanism picks up the gun tip and transfers the sample to the corresponding consumables by adsorption.

[0026] The carrier driving mechanism drives the carrier to move, and the consumables pass through the corresponding reagent adding tubes in sequence, and the reagent adding tubes add reaction reagents into the consumables in sequence;

[0027] After the carrier moves to the second position, the sample extraction module moves to each consumable in turn to operate until the eluent is obtained, and the sample extraction module is oscillated and the carrier is heated;

[0028] The pipetting mechanism picks up the tip and transfers the eluate into a PCR tube, and then transfers the PCR tube to the PCR module for PCR amplification detection.

[0029] The above scheme of the present invention has the following beneficial effects:

[0030] The sample detection integrated machine and detection method provided by the present invention have a sample loading module that can match the assembly line conveying, automatically complete the picking up of sample tubes and the transfer of samples, a consumable loading module that can automatically complete the sequential loading of consumables, and a reagent loading module that can automatically complete the addition of multiple reaction reagents, and consumables and reagents can be pre-added to consumable bins, reagent barrels, etc., and no manual replenishment is required during the assembly line extraction and detection process, so that the entire device is adapted to the automated assembly line, effectively improving the efficiency of large-scale sample extraction and detection, reducing manual labor intensity, and thus reducing the cost of sample extraction and detection; the consumable loading module can ensure the orderly discharge of consumables, that is, the sequential discharge method is convenient for the handling manipulator to pick up materials, and is further adapted to the automated assembly line; the reagent loading module adopts a non-contact liquid addition mode, and does not need to contact the solution in the consumables and the side walls of the consumables, so it can be reused and will not interfere with the next nucleic acid detection result, thereby simplifying the action setting, improving the efficiency of reagent addition, and further adapting to the automated assembly line;

[0031] Other beneficial effects of the present invention will be described in detail in the subsequent specific implementation section. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0033] Figure 2 It is a schematic diagram of a sample loading module of the present invention;

[0034] Figure 3 It is a schematic diagram of the consumable loading module of the present invention;

[0035] Figure 4 This is a schematic diagram of the installation of the reagent adding tube of the present invention;

[0036] Figure 5 It is a schematic diagram of the sample extraction module and the reaction carrying module of the present invention;

[0037] Figure 6 It is a schematic diagram of the material transfer module, gun tip rack and PCR tube of the present invention.

[0038] [Description of Reference Numerals]

[0039] 100-sample loading module; 110-sample tube; 120-sample conveying line; 130-sample picking mechanism; 131-sample picking claw; 132-first finger cylinder; 133-rotating shaft; 134-rotating shaft seat; 135-base; 136-first motor; 137-first synchronous belt; 138-first synchronous wheel; 139-lifting guide rail; 1310-second motor; 1311-second synchronous wheel; 1312-second synchronous belt; 140-sample fixing mechanism; 141-sample clamp; 142-sample clamp arm; 143-second finger cylinder; 144-placing seat; 200-consumable loading module; 201-deep well plate; 202-consumable bin; 203-feeding slot; 204-feeding plate; 205-feeding mounting seat; 206-feeding screw rod; 207-feeding driving motor; 208-feeding guide rail; 3 00-reagent loading module; 301-reagent adding tube; 302-reagent tube rack; 303-reagent barrel; 304-peristaltic pump; 400-sample extraction module; 410-magnetic rod sleeve assembly; 411-first driving mechanism; 412-first mounting bracket; 413-magnetic rod sleeve; 414-magnetic rod sleeve rack; 420-magnetic rod assembly; 421-second driving mechanism; 422-second mounting bracket; 423-magnetic rod; 431-third driving mechanism; 432-driving seat; 500-material transfer module; 501-handling robot; 502-liquid transfer mechanism; 503-cover opening robot; 504-material transfer drive mechanism; 600-PCR module; 601-PCR tube; 700-reaction carrying module; 701-carrying platform; 702-carrying platform drive mechanism; 801-gun tip; 802-gun tip material removal rack; 803-gun tip discard rack. DETAILED DESCRIPTION

[0040] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following will be described in detail with reference to the accompanying drawings and specific embodiments. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0041] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0042] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a locking connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0043] like Figure 1-Figure 6As shown, an embodiment of the present invention provides a sample detection integrated machine, including a chassis and a plurality of functional modules arranged in the chassis, and these functional modules specifically include a sample loading module 100, a consumable loading module 200, a reagent loading module 300, a sample extraction module 400, a material transfer module 500 and a PCR module 600. Among them, the sample loading module 100 docks with the sample conveying line, picks up and transfers the sample tube 110 to be detected, and the sample in the sample tube 110 is transferred to the consumables through the material transfer module 500. In this embodiment, the consumables take the deep well plate 201 as an example, and the deep well plate 201 is used as a reaction container for loading samples, reagents and reaction sites. After a group of samples are tested, a new deep well plate 201 needs to be replaced to avoid interference with subsequent tests. The consumable loading module 200 is used to continuously load the deep well plate 201 to a preset position, which is convenient for the material transfer module 500 to pick up and transport it to the operating position, and the consumable loading module 200 can pre-store a large number of deep well plates 201, and no manual addition is required in the subsequent assembly line extraction and detection process. The reagent loading module 300 is used to automatically load the corresponding reagents into the deep well plate 201. Taking nucleic acid extraction detection as an example, some deep wells of the deep well plate 201 are filled with samples to be extracted and tested, and the reagent loading module 300 adds a lysis reagent to the sample to lyse the nucleic acid and adsorb it on the magnetic beads. The magnetic beads are transferred to other deep wells by relying on the sample extraction module 400. These deep wells are filled with washing reagents added by the reagent loading module 300, and the nucleic acids adsorbed by the magnetic beads are washed with proteins. The magnetic beads are then transferred to other deep wells, and these deep wells are filled with elution reagents added by the reagent loading module 300. The nucleic acids adsorbed by the magnetic beads are eluted into eluents, and then the eluents are transferred, piped, and placed in the PCR module 600 for amplification detection through the material transfer module 500. The reagent loading module 300 also adopts a method of automatically adding reaction reagents, and no manual supplementation is required during the assembly line extraction and detection process, so that the entire device is adapted to the automated assembly line.

[0044] The sample detection integrated machine provided in this embodiment, including each functional module including the sample loading module 100, the consumables loading module 200, and the reagent loading module 300, all operates in an automated manner and can be adaptively connected to the assembly line, thereby ensuring the efficiency and reliability of large-scale sample extraction and detection.

[0045] Please refer again Figure 2In this embodiment, the sample loading module 100 includes a sample conveying line 120, a sample picking mechanism 130 and a sample fixing mechanism 140. The sample conveying line 120 adopts the form of track conveying, and the sample conveying line 120 is connected to the assembly line or is itself a part of the assembly line. The sample tube 110 containing the sample is conveyed along the sample conveying line 120 to one side of the sample picking mechanism 130, and the sample picking mechanism 130 picks up the sample tube 110 and transfers it to the sample fixing mechanism 140. The sample fixing mechanism 140 fixes the sample tube 110, and the material transfer module 500 transfers the sample in the sample tube 110 to the deep well plate 201, that is, the automatic loading of the sample is completed.

[0046] The sample picking mechanism 130 includes a sample picking jaw 131, a first finger cylinder 132, a rotating shaft 133, and a picking drive unit. The sample picking jaw 131 is arranged at the output end of the first finger cylinder 132, and a pair of the sample picking jaws 131 are arranged, and the opening and closing are controlled by the first finger cylinder 132. The first finger cylinder 132 is fixedly connected to the first end of the rotating shaft 133, and the rotating shaft 133 is arranged for vertical rotation. The second end of the rotating shaft 133 is rotatably connected to the rotating shaft seat 134. When the rotating shaft 133 rotates, it drives the first finger cylinder 132 to rotate, so that the first finger cylinder 132 and the sample picking jaw 131 have a degree of freedom of rotation around the vertical direction, and can rotate to the top of the sample conveying line 120 to pick up the sample tube 110, and rotate to the top of the sample fixing mechanism 140 to place the sample tube 110. The pickup drive unit includes a base 135, on which a first motor 136 is disposed. The first motor 136 is connected to the rotating shaft 133 through a first synchronous belt 137 and a first synchronous wheel 138 to drive the rotating shaft 133 to rotate. At the same time, a lifting rail 139, a second motor 1310, a second synchronous wheel 1311 and a second synchronous belt 1312 are also disposed on the base 135. The rotating shaft seat 134 is slidably connected to the lifting rail 139. The second motor 1310 drives the second synchronous wheel 1311 to rotate so that the second synchronous belt 1312 is circulated and transported. The second synchronous belt 1312 is connected to the rotating shaft seat 134 to drive the rotating shaft seat 134 to rise and fall along the lifting rail 139, thereby adjusting the height position of the first finger cylinder 132 and the sample picking clamp 131 to adapt to the height position of clamping and placing the sample tube 110. It should be noted that, since the rotating shaft 133 is raised and lowered synchronously, and the first synchronous wheel 138 does not rise and fall with the rotating shaft 133, the first synchronous wheel 138 and the rotating shaft 133 cooperate in such a way that the rotating shaft 133 can be displaced relative to the first synchronous wheel 138, and the first synchronous wheel 138 drives the rotating shaft 133 to rotate synchronously, and transmission can be achieved by using a spline or the like.

[0047] The sample fixing mechanism 140 includes a sample chuck 141, a sample clamp arm 142, a second finger cylinder 143 and a placement seat 144. The sample chuck 141 is fixed to the first end of the sample clamp arm 142, and the second end of the sample clamp arm 142 is connected to the output end of the second finger cylinder 143. The sample chuck 141 and the sample clamp arm 142 are each provided in a pair, and the second finger cylinder 143 controls the opening and closing of the sample chuck 141 to clamp or loosen the sample tube 110. The placement seat 144 is located between the sample chucks 141 to carry the placed sample tube 110. After the sample fixing mechanism 140 clamps the sample tube 110, the material transfer module 500 can rotate the tube cover of the sample tube 110 to open it, and then transfer the sample to the deep well plate 201 to prepare for nucleic acid extraction.

[0048] After the sample in the sample tube 110 is transferred, the sample fixing mechanism 140 releases the sample tube 110, and the sample picking mechanism 130 can pick up the sample tube 110 and transfer it to the sample conveying line 120 in the opposite manner to the above, or transfer it to a sample tube collection box for placement, etc., so that the sample tube 110 can be recovered, all of which are completed in an automated manner.

[0049] Please refer again Figure 3 In this embodiment, the consumable loading module 200 includes a consumable bin 202, in which consumables such as a deep-hole plate 201 are placed. At the same time, a material-discharging slot 203 is provided on the bottom surface and one of the side surfaces of the consumable bin 202, and is connected to form an L-shaped material-discharging slot 203, and a material-discharging mechanism is provided on the side corresponding to the side surface. Among them, the material discharging mechanism includes a material discharging plate 204, a material discharging mounting seat 205 fixedly connected to the material discharging plate 204, a material discharging screw rod 206 and a material discharging driving motor 207. The material discharging driving motor 207 is connected to the material discharging screw rod 206 to drive the material discharging screw rod 206 to rotate. The material discharging screw rod 206 cooperates with the threaded hole on the material discharging mounting seat 205, so that the material discharging mounting seat 205 drives the material discharging screw rod 206 to move along the material discharging screw rod 206 during rotation, so that the material discharging plate 204 enters from the material discharging slot 203 on the bottom surface of the consumable bin 202, and the deep hole plate 201 is pushed upward in turn, so that the deep hole plate 201 discharges materials from the top of the consumable bin 202 in turn, which is convenient for the material moving module 500 to pick up. Of course, the material discharging mounting seat 205 and the material discharging plate 204 can also be regarded as an integrated structure, that is, a part of the material discharging plate 204, and the material discharging screw rod 206 directly drives the material discharging plate 204 to rise and fall. In addition, when all the deep hole plates 201 in the consumable bin 202 have been taken out, it is necessary to replenish the deep hole plates 201 to the consumable bin 202. At this time, the material stripping plate 204 has moved to the highest position. Under the premise of setting only a single material stripping plate 204, it is necessary to drive the material stripping plate 204 to reset and withdraw from the material stripping slot 203. This is achieved by driving the material stripping drive motor 207 to drive the material stripping screw 206 to rotate in the opposite direction. At this time, the material stripping plate 204 can be directly driven to move to the lowest position without the need to gradually control the material stripping plate 204 to move downward.

[0050] Among them, the material-pickling screw 206, the material-pickling mounting seat 205, etc. are all arranged outside the consumable bin 202 to avoid occupying the internal space of the consumable bin 202. Based on the driving form of the material-pickling screw 206, it needs to be arranged on one side of the consumable bin 202 and parallel to the consumable bin 202 to ensure that the compactness of the structure is achieved while the material-pickling plate 204 has a sufficient stroke. Therefore, in order to enable the material-pickling mounting seat 205 located outside the consumable bin 202 to drive the material-pickling plate 204 to enter the consumable bin 202, in this embodiment, a material-pickling notch 203 is also opened on the side of the consumable bin 202, which is connected to the material-pickling notch 203 on the bottom surface of the consumable bin 202, so that the entire material-pickling notch 203 is L-shaped. When the material stripping plate 204 is lifted or lowered inside the consumable bin 202, the end of the material stripping plate 204 extends out from the material stripping slot 203 on the side of the consumable bin 202 and is connected to the material stripping mounting seat 205, ensuring that the material stripping plate 204 is lifted or lowered smoothly and can move along the consumable bin 202 to strip materials.

[0051] In addition, the consumable loading module 200 also includes a material-dipping guide rail 208, and the material-dipping mounting seat 205 is slidably connected to the material-dipping guide rail 208 via a slider, so that the material-dipping mounting seat 205 moves more smoothly and the deep-hole plate 201 in the consumable bin 202 is more reliably supported during material dipping.

[0052] The material discharging drive motor 207 is used to control the material discharging plate 204 to move a preset distance toward the top of the consumable bin 202 at a time, so that a preset number of deep-hole plates 201 are discharged and taken away, thereby ensuring the orderly discharge of the deep-hole plates 201. Since the volume of the consumable bin 202 is large enough, there is no need to manually replenish the deep-hole plates 201 in the middle of the assembly line operation, and it is suitable for automated assembly lines.

[0053] Please refer again Figure 4 In this embodiment, the reagent loading module 300 adopts a non-contact liquid addition mode, which is different from the traditional method. Specifically, the reagent loading module 300 includes a reagent addition tube 301 and a reagent tube rack 302 that supports and fixes the reagent addition tube 301. In this embodiment, the reagent addition tube 301 is fixedly set, and the reagent addition tube 301 is connected to the reagent barrel 303 through an infusion pump. The infusion pump is used to pump the reaction reagent in the reagent barrel 303 to the reagent addition tube 301, so that the reagent addition tube 301 introduces the reaction reagent into the deep hole after aligning the deep hole. When adding reaction reagents through the reagent addition tube 301, the reagent addition tube 301 does not need to contact the solution in the deep hole and the side wall of the deep hole, so it can be reused and will not interfere with the next nucleic acid test result. Relying on the setting of the reagent addition tube 301, the corresponding reaction reagent can be directly added to the deep hole, which simplifies the action setting and improves the efficiency of reagent addition.

[0054] As a preferred implementation of this embodiment, the infusion pump is in the form of a peristaltic pump 304, and each reagent addition tube 301 can be connected to a peristaltic pump 304. The advantage of the peristaltic pump 304 is that the liquid delivery is highly accurate, and it is adapted to the requirements of sample detection, and can accurately add a preset amount of reaction reagent to the deep hole. In this embodiment, the reagent barrel 303 containing different reagents and the corresponding reagent addition tube 301 are connected by different pipelines. When the corresponding reagent needs to be transported, the corresponding peristaltic pump 304 is started for transportation. Therefore, the reagent to be added can be pre-stored in the reagent barrel 303, and the volume of the reagent barrel 303 can be set to be very large. After the assembly line operation starts, there is no need to manually add reagents midway, and it is also adapted to the automated assembly line.

[0055] Since the deep well plate 201 has multiple rows of deep holes, it is necessary to add corresponding reagents to each row of deep holes. In order to reduce the space volume occupied by the fixed reagent addition tube 301, the reagent addition tube 301 is only arranged in a single row in this embodiment. When adding reagents, the deep well plate 201 moves so that each row of deep holes of the deep well plate 201 passes through the reagent addition tube 301 in sequence, so that the corresponding amount of reagents are added to all the deep holes of the deep well plate 201. In addition, as mentioned above, it is necessary to add lysis reagents, washing reagents and elution reagents in sequence during nucleic acid extraction, so at least three groups of different deep holes need to be added for three reactions. As a preferred embodiment, each reaction reagent in this embodiment corresponds to a deep well plate 201, that is, a group of nucleic acid extraction and detection operations are performed simultaneously through three deep well plates 201, the first deep well plate 201 is used to add lysis reagents and perform nucleic acid lysis, the second deep well plate 201 is used to add washing reagents and perform protein washing, and the third deep well plate 201 is used to add elution reagents and perform nucleic acid elution, which increases the number of one-time extractions, thereby improving the efficiency of sample extraction and detection. At the same time, three groups of reagent adding tubes 301 are also provided, and each group of reagent adding tubes 301 is used to add corresponding reaction reagents.

[0056] Please refer again Figure 5 , a reaction carrying module 700 is also provided in the chassis, and the reaction carrying module 700 includes a carrying platform 701 and a carrying platform driving mechanism 702. The carrying platform 701 is used to place the deep well plate 201, and multiple deep well plates 201 can be placed at the same time, so that the aforementioned three deep well plates 201 can be placed and reacted at the same time. In addition, a heating unit is also provided in the carrying platform 701, and the heating unit is used to generate heat to heat the solution in the deep well plate 201 to ensure the high temperature required for the reaction between the sample and the reagent. The carrying platform driving mechanism 702 can adopt the common forms in the prior art such as guide rail sliders and screw rods or belt drives, and the specific structural settings will not be repeated here.

[0057] Please refer again Figure 5In this embodiment, the sample extraction module 400 includes a magnetic rod sleeve assembly 410 and a magnetic rod assembly 420. The magnetic rod sleeve assembly 410 includes a first mounting frame 412 connected to a first driving mechanism 411 and a magnetic rod sleeve 413 installed on the first mounting frame 412. The first driving mechanism 411 is used to drive the first mounting frame 412 to move vertically. The magnetic rod assembly 420 includes a second mounting frame 422 connected to a second driving mechanism 421 and a magnetic rod 423 installed on the second mounting frame 422. The second driving mechanism 421 is used to drive the second mounting frame 422 to move vertically, thereby driving the magnetic rod 423 on the second mounting frame 422 to move vertically. The first driving mechanism 411 drives the first mounting frame 412 and the magnetic rod sleeve 413 on the first mounting frame 412 to move vertically, so that they are inserted into the deep well plate 201 to extract the sample, and at the same time, the sample, the lysis reagent, and the magnetic beads are evenly mixed by reciprocating oscillation. After the uniform mixing is completed, the second driving mechanism 421 drives the second mounting frame 422 and the magnetic rod 423 to be inserted into the magnetic rod sleeve 413 one by one, so that the magnetic beads are adsorbed on the surface of the magnetic rod sleeve 413. Subsequently, the magnetic rod sleeve 413 adsorbed with the magnetic beads is sequentially inserted into the deep hole containing the washing reagent and the deep hole containing the elution reagent, and the protein washing and nucleic acid elution processes are performed respectively, so that the nucleic acid is retained in the elution solution for subsequent PCR.

[0058] It is understandable that the magnetic rod sleeve assembly 410 and the magnetic rod assembly 420 as a whole need to be moved and switched to the top of different deep holes, so the sample extraction module 400 also includes a third driving mechanism 431, and the third driving mechanism 431 is connected to the driving seat 432. The first driving mechanism 411 and the second driving mechanism 421 are both arranged on the driving seat 432. The third driving mechanism 431 is used to horizontally displace the driving seat 432, so that the magnetic rod sleeve assembly 410 and the magnetic rod assembly 420 as a whole can be horizontally displaced to switch to the top of different deep holes.

[0059] It is understandable that the outer surface of the magnetic rod sleeve 413 will come into contact with the liquid in the deep hole during the extraction process, so it will adhere to the liquid containing nucleic acid, etc. This may affect the test results during the next group of nucleic acid extraction tests. Based on this, the present embodiment adopts a solution of replacing the magnetic rod sleeve 413, that is, the magnetic rod sleeve 413 itself is also used as a consumable, and a new magnetic rod sleeve 413 is replaced after each group of nucleic acid extraction tests are completed. Among them, a magnetic rod sleeve rack 414 is also provided in the chassis, and multiple magnetic rod sleeves 413 are placed on the magnetic rod sleeve rack 414, or the magnetic rod sleeve 413 also adopts a solution of conveying by a feeding bin, and then relies on the material transfer module 500 to pick up and install it on the first mounting frame 412.

[0060] Of course, in other embodiments, a cleaning solution for the magnetic rod cover 413 may be used to clean the residual liquid on the outer surface of the magnetic rod cover 413 after completing a set of nucleic acid extraction tests, which also will not affect subsequent test results.

[0061] Please refer again Figure 6 In this embodiment, after the nucleic acid is eluted, the nucleic acid adsorbed on the surface of the magnetic beads is eluted and retained in the eluent, and the eluent needs to be transferred to the PCR tube 601, so that the PCR tube 601 can be moved to the PCR module 600 for PCR amplification detection. In this embodiment, the transfer of the eluent is still carried out by the gun tip 801. A gun tip material rack 802 and a gun tip discarding rack 803 are provided in the chassis. A new gun tip 801 is placed on the gun tip material rack 802, so that the material transfer module 500 can pick up the gun tip 801, absorb the eluent from the deep well plate 201, and then transfer it to the PCR tube 601. After completion, the gun tip 801 is detached and placed on the gun tip discarding rack 803. In addition, the sample in the sample tube 110 is transferred to the deep well plate 201 through the material transfer module 500 and the gun tip 801. The solution of using the gun tip 801 for transfer is also a prior art, which will not be repeated here.

[0062] Please refer again Figure 6 In this embodiment, the material transfer module 500 includes a handling manipulator 501, a liquid transfer mechanism 502, and a cover opening manipulator 503, etc., which are all arranged on a material transfer drive mechanism 504. The material transfer drive mechanism 504 has multiple degrees of freedom of translation in the longitudinal, transverse and vertical directions, so that the handling manipulator 501, the liquid transfer mechanism 502, and the cover opening manipulator 503 can move to the corresponding position, and then adjust the height to perform the operation, and the cover opening manipulator completes the cover opening of the sample tube 110 at the sample loading module 100, and the liquid transfer mechanism 502 picks up the gun head 801 to complete the liquid transfer of the sample, and the handling manipulator 501 completes the transfer of the deep well plate 201 and the transfer of the magnetic rod sleeve 413, and the liquid transfer mechanism 502 transfers the eluate to the PCR tube 601. The material transfer drive mechanism 504 can also adopt the common forms in the prior art such as guide rail sliders and screw rods or belt drives, and the specific structural settings are not repeated here.

[0063] The sample detection integrated machine provided in this embodiment has the sample conveying line 120 docked with the assembly line after installation, the consumables bin 202 is loaded with a sufficient number of deep-well plates 201, and the reagent barrel 303 is loaded with a corresponding amount of reagents. During operation, the sample picking mechanism 130 picks up each sample tube 110 from the sample conveying line 120 and fixes it to the sample fixing mechanism 140. After the cover opening manipulator 503 opens the tube cover of the sample tube 110, the liquid transfer mechanism 502 picks up the gun tip 801 and transfers the sample to the corresponding deep-well plate 201 by adsorption. Prior to this, the deep-well plate 201 has been loaded through the consumables bin 202 and transferred to the carrier 701 of the reaction carrier module 700 by the transport manipulator 501, and the carrier 701 is in the first position at this time. After the sample is transferred to the deep-well plate 201, the carrier driving mechanism 702 drives the carrier 701 to move, and each row of deep holes of the deep-well plate 201 passes through the corresponding reagent adding tube 301 in turn, and the reagent adding tube 301 adds reaction reagents to each row of deep holes in the deep-well plate 201 in turn, and one of the deep-well plates 201 is a mixed solution of lysis reagent, sample and magnetic beads. After the carrier 701 moves to the second position, the sample extraction module 400 moves to the top of each deep-well plate 201 in turn to operate until the eluent is obtained. In this process, the sample extraction module 400 is relied on to oscillate and the carrier 701 is heated to ensure that the reaction is sufficient and smooth. Finally, the liquid transfer mechanism 502 picks up the gun head 801 and transfers the eluent to the PCR tube 601, and then transfers the PCR tube 601 to the PCR module 600 for PCR amplification detection, that is, the extraction and detection of the sample is fully automated, which is suitable for large-scale, assembly-line detection.

[0064] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0065] The above embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the attached claims.

Claims

1. A sample detection integrated machine, characterized in that: It comprises a sample loading module (100), a consumable material loading module (200), a reagent loading module (300), and a material transfer module (500); The sample loading module (100) comprises a sample conveying line (120), a sample picking mechanism (130) and a sample fixing mechanism (140); the sample conveying line (120) corresponds to the assembly line and is used to convey a sample tube (110) containing a sample; the sample picking mechanism (130) is used to pick up the sample tube (110) on the sample conveying line (120) and transfer it to the sample fixing mechanism (140); the sample fixing mechanism (140) is used to fix the sample tube (110); and the material transfer module (500) is capable of transferring the sample in the sample tube (110) to a consumable; The consumable material loading module (200) comprises a consumable material bin (202) and a material shifting mechanism, the consumable material bin (202) is filled with the consumable material, the material shifting mechanism is used to shift the consumable material in the consumable material bin (202) to the material outlet of the consumable material bin (202) in sequence, and the material shifting module (500) is capable of shifting the consumable material at the material outlet; The reagent loading module (300) comprises a reagent adding tube (301) and a reagent storage container, wherein the reagent adding tube (301) is connected to the reagent storage container via an infusion section, wherein the infusion section is used to transport the reaction reagent in the reagent storage container to the reagent adding tube (301), and the reagent adding tube (301) is used to introduce the reaction reagent into the consumable.

2. The sample detection integrated machine according to claim 1, characterized in that: The sample picking mechanism (130) comprises a sample picking jaw (131), a first finger cylinder (132), a rotating shaft (133), a rotating shaft seat (134), and a picking drive unit. The sample picking jaw (131) is arranged at the output end of the first finger cylinder (132), and at least one pair is arranged. The first finger cylinder (132) is fixedly connected to the rotating shaft (133), and the rotating shaft (133) is rotatably connected to the rotating shaft seat (134). The picking drive unit is used to drive the rotating shaft (133) to rotate and rise and fall.

3. The sample detection integrated machine according to claim 2, characterized in that: The pickup drive unit comprises a base (135), on which a first motor (136) is arranged, the first motor (136) being transmission-connected to the rotating shaft (133) via a first synchronous belt (137) and a first synchronous wheel (138), the base (135) also being provided with a lifting guide rail (139), a second motor (1310), a second synchronous wheel (1311) and a second synchronous belt (1312), the rotating shaft seat (134) being slidingly connected to the lifting guide rail (139), the second motor (1310) being transmission-connected to the second synchronous wheel (1311), the second synchronous belt (1312) being wound around the second synchronous wheel (1311), and the second synchronous belt (1312) being fixedly connected to the rotating shaft seat (134).

4. The sample detection integrated machine according to claim 1, characterized in that: The sample fixing mechanism (140) comprises a sample clamp (141), a sample clamp arm (142), a second finger cylinder (143) and a placement seat (144); the sample clamp (141) is arranged at the first end of the sample clamp arm (142); the second end of the sample clamp arm (142) is connected to the output end of the second finger cylinder (143); the sample clamp (141) and the sample clamp arm (142) are each arranged in a pair; and the placement seat (144) is located between the sample clamps (141).

5. The sample detection integrated machine according to claim 1, characterized in that: The consumable material bin (202) is provided with a material shifting slot (203), and the material shifting mechanism comprises a material shifting plate (204), a material shifting mounting seat (205) fixedly connected to the material shifting plate (204), and a material shifting driving unit for driving the material shifting mounting seat (205) and the material shifting plate (204) to shift materials toward the material discharge port. The material shifting plate (204) matches the size of the material shifting slot (203), enters from the material shifting slot (203) and moves toward the material discharge port to shift materials.

6. The sample detection integrated machine according to claim 1, characterized in that: The infusion part is a peristaltic pump (304), and the peristaltic pump (304) and the reagent adding tube (301) are arranged in a one-to-many correspondence, and each peristaltic pump (304) is corresponding to the delivery of a reaction reagent; Alternatively, the peristaltic pumps (304) and the reagent adding tubes (301) are arranged in a one-to-one correspondence, and each group of the peristaltic pumps (304) conveys a corresponding reaction reagent; Each of the peristaltic pumps (304) is independently controlled.

7. The sample detection integrated machine according to claim 1, characterized in that: The sample detection integrated machine further comprises a reaction carrying module (700), wherein the reaction carrying module (700) comprises a carrying platform (701) and a carrying platform driving mechanism (702), wherein the carrying platform (701) is used for placing consumables, and the carrying platform driving mechanism (702) is used for driving the carrying platform (701) to move.

8. The sample detection integrated machine according to claim 1, characterized in that: The sample extraction module (400) comprises a magnetic rod sleeve assembly (410) and a magnetic rod assembly (420); The magnetic rod sleeve assembly (410) comprises a first driving mechanism (411), a first mounting frame (412) connected to the first driving mechanism (411), and a magnetic rod sleeve (413) mounted on the first mounting frame (412); the magnetic rod assembly (420) comprises a second driving mechanism (421), a second mounting frame (422) connected to the second driving mechanism (421), and a magnetic rod (423) mounted on the second mounting frame (422), the magnetic rod (423) being located directly above the magnetic rod sleeve (413), and the magnetic rod (423) and the magnetic rod sleeve (413) being arranged correspondingly; The sample extraction module (400) further comprises a third driving mechanism (431) and a driving seat (432); the third driving mechanism (431) is connected to the driving seat (432); and the first driving mechanism (411) and the second driving mechanism (421) are both arranged on the driving seat (432).

9. The sample detection integrated machine according to claim 1, characterized in that: The material transfer module (500) comprises a transport robot (501), a liquid transfer mechanism (502), a cover opening robot (503) and a material transfer drive mechanism (504); the transport robot (501), the liquid transfer mechanism (502) and the cover opening robot (503) are all connected to the material transfer drive mechanism (504); the transport robot (501) is used to transport the consumables, the liquid transfer mechanism (502) is used to transfer the solution, and the cover opening robot (503) is used to open the tube cover of the sample tube (110); The sample detection machine also includes a gun tip retrieval rack (802) and a gun tip discard rack (803), wherein the gun tip retrieval rack (802) is used to place new gun tips (801), and the gun tip discard rack (803) is used to place discarded gun tips (802), and the pipetting mechanism (502) transfers liquid through the gun tips (801).

10. A sample detection method, using the sample detection integrated machine as claimed in any one of claims 1 to 9, characterized in that: include: The consumables are pre-loaded in the consumable bin (202), and the reagents are pre-loaded in the reagent barrel (303). The consumables are loaded through the consumable bin (202), and the transport robot (501) transfers them to the carrier platform (701) of the reaction carrier module (700), and the carrier platform (701) is in the first position; The sample picking mechanism (130) picks up each sample tube (110) from the sample conveying line (120) and fixes it to the sample fixing mechanism (140), the cover opening robot (503) opens the tube cover of the sample tube (110), and the pipetting mechanism (502) picks up the gun tip (801) and transfers the sample to the corresponding consumable by adsorption; The carrier platform driving mechanism (702) drives the carrier platform (701) to move, and the consumables pass through the corresponding reagent adding tubes (301) in sequence, and the reagent adding tubes (301) add reaction reagents into the consumables in sequence; After the carrier (701) moves to the second position, the sample extraction module (400) moves to each consumable in turn to perform operations until the eluent is obtained, and the sample extraction module (400) is oscillated and the carrier (701) is heated; The pipetting mechanism (502) picks up the gun tip (801) and transfers the eluate to the PCR tube (601), and then transfers the PCR tube (601) to the PCR module (600) for PCR amplification detection.

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