Sample analyzer and sample detection method

Abstract

The invention discloses a sample analyzer and a sample detection method, according to the sample analyzer provided by the invention, reagents are added into a reaction tank in a time-sharing manner, so that a to-be-detected sample treated by a first reagent can be prevented from being influenced by a second reagent, and different cell parameters in the to-be-detected sample can be measured in a time-sharing manner; required measurement data can be obtained by one sample to be detected, the detection efficiency is improved, and the reagent cost is saved.

Description

Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a sample analyzer and a sample detection method. Background Art

[0002] In the prior art, electrical impedance is generally used to measure RBC (Erythrocyte / Red Blood Cell) and PLT (Platelet) counts and their volume size. However, the platelet counting process is affected by small-volume red blood cells, red blood cell fragments, etc., and it is impossible to identify large platelets, small red blood cells, red blood cell fragments or others, which affects the measurement of cell parameters in the blood sample.

[0003] The prior art further proposes to use optical detection to detect blood samples and to use reagents to spheronize the cells to change the flat state of red blood cells, thereby achieving the purpose of distinguishing red blood cells from platelets. In order to further distinguish the cells in the optical detection, dye solution is generally added to dye the cells, but the addition of dye solution will affect the size of the red blood cells after spheronization, thereby affecting the red blood cell nine-point diagram, and cannot truly reflect the typing of red blood cells.

[0004] Furthermore, in the process of testing the blood sample to obtain the cell parameters such as platelets and red blood cells, the blood sample needs to be tested more than twice to respectively measure the parameters of different cells in the blood sample, which is complicated. Summary of the invention

[0005] In order to solve the above technical problems, the present application provides a sample analyzer, including a reaction pool, a pipetting component, an optical detection component and a sheath liquid supply component, wherein the pipetting component and the reaction pool are spaced apart; the reaction pool is connected to a flow chamber of the optical detection component, and the sheath liquid supply component is connected to the flow chamber; Wherein, the pipetting component is used to inject the sample to be tested and the first reagent into the reaction pool to form a first sample liquid; The sheath liquid supply component is used to supply sheath liquid to the flow chamber of the optical detection component to form a sheath flow in the flow chamber; The optical detection component is used to perform optical detection on a portion of the first sample liquid to obtain a detection result of at least one of red blood cells, platelets and hemoglobin in the sample to be tested; The liquid transfer assembly is also used to inject a second reagent into the reaction pool so that the remaining first sample liquid and the second reagent form a second sample liquid; The optical detection component is also used to perform optical detection on the second sample liquid to obtain a detection result of at least one of reticulocytes and immature platelets in the sample to be tested.

[0006] Wherein, the sample analyzer further comprises a sample pipeline connecting the reaction pool and the flow chamber, and a power component connected to the sample pipeline; The power component is used to extract part of the first sample liquid in the reaction pool into the sample pipeline, and after the sheath liquid supply component forms a stable sheath flow in the flow chamber, push the first sample liquid in the sample pipeline into the flow chamber; The power assembly is also used to push the second sample liquid in the reaction pool to the flow chamber via the sample pipeline after the first sample liquid is detected and the sample pipeline is cleaned.

[0007] Wherein, the sample analyzer further comprises a first valve, and the first valve is arranged on the sample pipeline; After the power assembly draws part of the first sample liquid in the reaction pool into the sample pipeline, the first valve is disconnected, and the sheath liquid supply assembly is used to supply sheath liquid to the flow chamber to establish the sheath flow; After the sheath liquid supply assembly forms a stable sheath flow in the flow chamber, the first valve is turned on, and the power assembly pushes the first sample liquid in the sample pipeline into the flow chamber.

[0008] Wherein, the power assembly includes a first power component and a second power component; the first power component is connected to one end of the sample pipeline close to the flow chamber, and the second power component is connected to one end of the sample pipeline close to the reaction pool; The first power component is used to extract part of the first sample liquid or the second sample liquid in the reaction pool into the sample pipeline, and make the first end of the first sample liquid or the second sample liquid be located in the pipeline between the flow chamber and the first power component; The second power component is used to push the first sample liquid or the second sample liquid in the pipeline between the reaction pool and the flow chamber into the flow chamber.

[0009] Wherein, the sample pipeline includes a first pipeline and a second pipeline connected in parallel; The first pipeline is provided with the first valve, and the second pipeline is provided with the second valve; The power assembly is used to draw part of the first sample liquid in the reaction pool into the first pipeline when the first valve is turned on and the second valve is turned off, and push the first sample liquid in the first pipeline into the flow chamber; The power assembly is also used to draw the second sample liquid in the reaction pool into the second pipeline when the first valve is cut off and the second valve is turned on, and push the second sample liquid in the second pipeline into the flow chamber.

[0010] Wherein, the length of the pipeline section from the connection between the first pipeline and the reaction tank to the first valve is equal to the length of the pipeline section from the connection between the second pipeline and the reaction tank to the second valve; And / or, the length of the pipeline section from the connection point of the first pipeline and the flow chamber to the first valve is equal to the length of the pipeline section from the connection point of the second pipeline and the flow chamber to the second valve; And / or, the length of the pipeline section between the reaction pool and the flow chamber on the first pipeline is equal to the length of the pipeline section between the reaction pool and the flow chamber on the second pipeline.

[0011] Wherein, the sample analyzer further includes a liquid storage tube and a third valve, and the power assembly further includes a third power component. The reaction pool is connected to the sample pipeline through the third valve, one end of the liquid storage tube is connected to the reaction pool through the third valve, and the third power component is connected to the other end of the liquid storage tube; After the first sample liquid is formed in the reaction pool, the third valve is switched to connect the liquid storage tube and the reaction pool, and the third power component is used to extract part of the first sample liquid in the reaction pool into the liquid storage tube.

[0012] After the first sample liquid is poured into the liquid storage tube, the third valve is switched to connect the reaction pool and the sample pipeline, and the first power component is used to extract the remaining first sample liquid in the reaction pool into the sample pipeline; The third valve is switched for the second time so that the reaction pool is connected to the liquid storage tube, and the third power component is also used to push the first sample liquid in the liquid storage tube into the reaction pool.

[0013] Wherein, the first valve is arranged in the pipeline section between the reaction pool and the flow chamber, and the sample analyzer further comprises a diluent supply assembly, and the diluent supply assembly is connected to one end of the sample pipeline close to the reaction pool; The diluent supply component is used to supply diluent to the reaction pool to clean the reaction pool when the first valve is cut off and the sheath liquid supply component supplies sheath liquid to the flow chamber; The diluent supply assembly is also used to supply the diluent to the reaction pool, the sample pipeline and the flow chamber when the first valve is turned on, so as to clean the reaction pool, the sample pipeline and the flow chamber.

[0014] In order to solve the above technical problems, the present application also provides a sample testing method, which is applied to the sample analyzer as described above, comprising: Injecting the sample to be tested and the first reagent into the reaction pool to form a first sample solution, and transporting the first sample solution to the optical detection component for optical detection to obtain a detection result of at least one of red blood cells, platelets and hemoglobin in the sample to be tested; During the detection of the first sample liquid, a second reagent is injected into the reaction pool to form a second sample liquid, and after the detection of the first sample liquid is completed, the second sample liquid is transported to the optical detection component for optical detection to obtain a detection result of at least one of reticulocytes and immature platelets in the sample to be tested.

[0015] Beneficial effects of the present application: Different from the prior art, the sample analyzer provided by the present application includes a reaction pool, a pipetting assembly, an optical detection assembly, and a sheath liquid supply assembly. The pipetting assembly is spaced apart from the reaction pool, the reaction pool is connected to the flow chamber of the optical detection assembly, and the sheath liquid supply assembly is connected to the flow chamber. Among them, the pipetting assembly is used to inject the sample to be tested and the first reagent into the reaction pool to form a first sample liquid, the sheath liquid supply assembly is used to supply sheath liquid to the flow chamber of the optical detection assembly to form a sheath flow in the flow chamber, and the optical detection assembly is used to perform optical detection on part of the first sample liquid to obtain at least one detection result of red blood cells, platelets and hemoglobin in the sample to be tested. The pipetting assembly is also used to inject a second reagent into the reaction pool so that the remaining first sample liquid and the second reagent form a second sample liquid, and the optical detection assembly is also used to perform optical detection on the second sample liquid to obtain at least one detection result of reticulocytes and immature platelets in the sample to be tested. By adding reagents to the reaction pool at different times, the second reagent can be prevented from affecting the sample to be tested after being treated with the first reagent, and different cell parameters in the sample to be tested can be measured at different times, so that the required measurement data can be obtained with one sample to be tested, thereby improving detection efficiency, saving reagent costs, and enhancing the user experience of the sample analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. Among them: Figure 1 It is a structural schematic diagram of the first embodiment of the sample analyzer of the present application; Figure 2 is a structural schematic diagram of a second embodiment of the sample analyzer of the present application; Figure 3 It is a structural schematic diagram of the third embodiment of the sample analyzer of the present application.

[0017] Figure numerals: sample analyzer 1; reaction pool 11; flow chamber 12; sheath liquid supply assembly 13; sample pipeline 14; first pipeline 141; second pipeline 142; power assembly 15; first power component 151; second power component 152; third power component 153; first valve 16; second valve 17; third valve 18; liquid storage tube 19. DETAILED DESCRIPTION

[0018] The scheme of the embodiment of the present application is described in detail below in conjunction with the drawings of the specification.

[0019] In the following description, for the purpose of explanation rather than limitation, specific details such as specific system structures, interfaces, and technologies are provided to facilitate a thorough understanding of the present application.

[0020] Reference to "embodiments" in this application means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0021] The term "and / or" in this application is only a description of the association relationship of associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the objects associated before and after are in an "or" relationship. In addition, "many" in this article means two or more than two. In addition, the term "at least one" in this article means any combination of at least two of any one or more of a plurality of, for example, including at least one of A, B, and C, and can mean including any one or more elements selected from the set consisting of A, B, and C. In addition, the terms "first", "second", and "third" in this application are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of indicated technical features.

[0022] In the prior art, electrical impedance is generally used to measure the red blood cell (RBC) and platelet (PLT) counts and their volume in blood samples. However, the platelet counting process is affected by small-volume red blood cells, red blood cell fragments, etc., and it is not possible to identify large platelets, small red blood cells, red blood cell fragments, or others. Usually, in order to identify large platelets, small red blood cells, red blood cell fragments, or others, an optical detection channel for RBC / PLT is introduced to improve the distinction between red blood cells and platelets in blood samples and improve the accuracy of blood sample detection.

[0023] In the process of optically testing a blood sample to obtain the parameters of each cell in the blood sample, a spheroidizing diluent reagent is generally added to the blood sample to spheroidize the red blood cells in the blood sample, so that the cells in the blood sample can be distinguished during the testing process, and the cells (such as flat red blood cells) will not be irradiated with laser light in the flow chamber at different angles and postures to obtain different optical signals, thereby preventing the cells from being misclassified and affecting the accuracy of the cell parameter test.

[0024] In order to better distinguish cells during optical detection, FR dye is generally added to the blood sample after the cells are spheroidized to dye the cells in the blood sample and enhance the differences between the cells. However, the addition of FR dye will affect the size of the red blood cells after spheroidization, thereby affecting the red blood cell nine-point diagram and failing to truly reflect the typing of red blood cells.

[0025] Based on the above problems, this application provides a sample analyzer, please refer to Figure 1 , Figure 1 1 is a schematic structural diagram of a first embodiment of a sample analyzer of the present application. The sample analyzer 1 provided in the embodiment of the present application comprises a reaction pool 11 , a liquid transfer component (not shown), an optical detection component and a sheath liquid supply component 13 .

[0026] The liquid transfer assembly is spaced apart from the reaction pool 11 , the reaction pool 11 is connected to the flow chamber 12 of the optical detection assembly, and the sheath liquid supply assembly 13 is connected to the flow chamber 12 .

[0027] Among them, the pipetting component is used to inject the sample to be tested and the first reagent into the reaction pool 11, wherein the sample to be tested can be a blood sample, and the first reagent can be a spheroidizing diluent reagent to spheroidize the cells in the sample to be tested to form a first sample liquid; the sheath liquid supply component 13 is used to supply sheath liquid to the flow chamber 12 of the optical detection component to form a sheath flow in the flow chamber 12; the optical detection component is used to perform optical detection on a portion of the first sample liquid to obtain a detection result of at least one of red blood cells, platelets and hemoglobin in the sample to be tested.

[0028] Furthermore, the pipetting component is also used to inject a second reagent into the reaction pool 11, wherein the second reagent can be FR dye solution, etc., so that the remaining first sample liquid and the second reagent form a second sample liquid; the optical detection component is also used to perform optical detection on the second sample liquid to obtain the detection result of at least one of reticulocytes and immature platelets in the sample to be tested.

[0029] By first mixing the sample to be tested with the first reagent, the cells in the sample to be tested are sphericalized to improve the difference between cells, improve the recognition efficiency of each cell in the sample to be tested, improve the detection efficiency of cells in the sample to be tested, improve the accuracy of detecting at least one of red blood cells, platelets and hemoglobin, and improve the user experience of the sample analyzer 1. The sample analyzer 1 can also obtain the red blood cell nine-point diagram corresponding to the sample to be tested through the obtained detection results of red blood cells, platelets and hemoglobin, and can further judge the type of anemia corresponding to the sample to be tested.

[0030] Furthermore, after part of the first sample liquid is transported to the flow chamber 12 for optical detection, a second reagent is added to the reaction pool 11 so that the second reagent and the remaining first sample liquid form a second sample liquid. Since part of the first sample liquid is transported to the flow chamber for optical detection, at least one detection result of red blood cells, platelets and hemoglobin is obtained. Then, the addition of the second reagent that affects the volume of the cells does not affect the detection of red blood cells, platelets and hemoglobin in the sample to be tested, and the orderliness of the injection of the first reagent and the second reagent is achieved. By adding reagents to the reaction pool 11 in time-sharing, it is possible to avoid the second reagent from affecting the sample to be tested after being treated with the first reagent, and it is possible to measure different cell parameters in the sample to be tested in time-sharing, so that the required measurement data can be obtained with one sample to be tested, thereby improving the detection efficiency, saving reagent costs, and enhancing the user experience of the sample analyzer 1.

[0031] In one embodiment, after the optical detection component completes optical detection of the first sample liquid, the pipetting component injects a second reagent into the reaction pool 11 to form a second sample liquid with the second reagent and the remaining first sample liquid, and then the optical detection component performs optical detection on the second sample liquid.

[0032] In another embodiment, during the process of the optical detection component optically detecting the first sample liquid, the liquid transfer component injects the second reagent into the reaction pool 11, so that the second reagent and the remaining first sample liquid form a second sample liquid, that is, the formation time of the second sample liquid overlaps with the detection time of the first sample liquid at least partially, reducing the waiting time for the formation of the second sample liquid, thereby improving the detection efficiency of the sample analyzer 1 for the sample to be tested and improving the user experience of the sample analyzer 1. Furthermore, after the optical detection component completes the detection of the first sample liquid, the optical detection component can then detect the second sample liquid to obtain a complete detection result of the sample to be tested.

[0033] It is understandable that the user can set the start time of preparing the second sample liquid according to needs, and this application does not impose any limitation on this.

[0034] In summary, the sample analyzer 1 provided in the embodiment of the present application adopts the method of adding the first reagent and the second reagent to the reaction pool 11 in a time-sharing manner, and can detect at least one of the red blood cells, platelet hemoglobin, reticulocytes and immature platelets in the sample to be tested, without the need to separately detect different cells in the sample to be tested, thereby improving the efficiency of cell detection by the sample analyzer 1. At the same time, by setting the first reagent to be added to the sample to be tested to form a first sample liquid, and then adding the second reagent to the reaction pool 11 during the detection of the first sample liquid, it is avoided that the added second reagent will affect the detection of red blood cells, platelets and hemoglobin, and the order of adding different reagents is reasonably planned, thereby further improving the detection accuracy of the sample analyzer 1 for the sample to be tested.

[0035] In one embodiment, if Figure 1 As shown, the reaction pool 11 can also be connected to a power pump through a valve, and then, after the pipetting component injects the sample to be tested and the first reagent into the reaction pool 11, the valve can be opened, and the power pump injects gas into the reaction pool 11 to mix the sample to be tested and the first reagent to form a first sample liquid. After the pipetting component injects the second reagent into the reaction pool 11, the power pump can also be turned on to mix the first sample liquid and the second reagent to form a second sample liquid, thereby improving the preparation efficiency of the first sample liquid and the second sample liquid, and improving the detection efficiency of the sample analyzer 1 for the first sample liquid and the second sample liquid.

[0036] In another embodiment, the sample analyzer 1 may further include an incubation component (not shown), which may be spaced apart from the reaction pool 11 to incubate the sample to be tested and the first reagent, or the first sample liquid and the second reagent in the reaction pool 11 to form a first sample liquid or a second sample liquid.

[0037] Optionally, the sample analyzer 1 further includes a sample pipeline 14 connecting the reaction pool 11 and the flow chamber 12 , and a power assembly 15 connected to the sample pipeline 14 .

[0038] The power assembly 15 is used to extract part of the first sample liquid in the reaction pool 11 into the sample pipeline 14 , and push the first sample liquid in the sample pipeline 14 into the flow chamber 12 after the sheath liquid supply assembly 13 forms a stable sheath flow in the flow chamber 12 .

[0039] The power assembly 15 is also used to push the second sample liquid in the reaction pool 11 to the flow chamber 12 through the sample pipeline 14 after the first sample liquid is detected and the sample pipeline 14 is cleaned.

[0040] Specifically, after the first sample liquid is formed in the reaction pool 11, the power component 15 extracts the first sample liquid in the reaction pool 11 into the sample pipeline 14. As mentioned above, the sample analyzer 1 includes an incubation component, which can incubate the sample to be tested and the first reagent in the reaction pool 11. In practice, incubating the sample to be tested means providing a specific temperature for the sample to be tested so that the sample to be tested is mixed with the first reagent at a specific temperature. After the sample to be tested and the first reagent are incubated to form the first sample liquid, if the first sample liquid continues to stay in the reaction pool 11 at a specific temperature, the first sample liquid may be incubated for too long, thereby damaging the cells in the sample to be tested, thereby affecting the test results. After the first sample liquid is formed, the power component 15 immediately extracts part of the first sample liquid into the sample pipeline 14.

[0041] After the sheath liquid supply component 13 forms a stable sheath flow in the flow chamber 12, the power component 15 pushes the first sample liquid in the sample line 14 into the flow chamber 12. The detection principle of the first sample liquid in the flow chamber 12 is that the sheath liquid carries the cells in the first sample liquid through the detection area in the flow chamber 12 one by one, and the optical detection component counts the cells according to the changes in the light beam when the cells pass through. A stable sheath flow is the basis of detection. When the sheath flow is unstable, the first sample may mix with the sheath liquid in the flow chamber 12 when it enters the flow chamber 12, and the sheath liquid cannot carry the cells in the first sample liquid through the detection area in the flow chamber 12 one by one, which affects the detection efficiency of the first sample liquid. Therefore, this embodiment proposes that the power component 15 pushes the first sample liquid in the sample line 14 into the flow chamber only after the sheath liquid supply component 13 forms a stable sheath flow in the flow chamber 12.

[0042] After the first sample liquid is detected, the sample analyzer 1 can first clean the sample line 14 and the flow chamber 12. As can be seen from the foregoing, during the detection of the first sample liquid, the pipetting component injects the second reagent into the reaction pool 11, and the first sample liquid and the second reagent are then incubated in the reaction pool 11 to form the second sample liquid. After the sample line 14 is cleaned and the second sample liquid is incubated, the power component 15 can extract the second sample into the sample line 14. Since the sheath liquid supply component 13 forms a stable sheath flow before the first sample liquid is detected, it does not stop supplying the sheath flow to the flow chamber 12 after the first sample liquid is detected, that is, a stable sheath flow is maintained in the flow chamber 12. Therefore, the power component 15 does not need to wait and can push the second sample liquid in the sample line 14 into the flow chamber 12.

[0043] It is understandable that, in the process of cleaning the sample line 14 and the flow chamber 12, specifically, a diluent may be introduced into the sample line 14 and the sample needle in the middle of the flow chamber 12 for cleaning. However, due to the structural limitation of the flow chamber 12, the outer wall of the flow chamber 12 is difficult to be cleaned. Therefore, in this embodiment, the sheath liquid supply assembly 13 is always in an open state, so that the flowing sheath liquid can maintain a stable sheath flow while also achieving the cleaning function of the outer wall of the flow chamber 12.

[0044] In one embodiment, the sheath liquid supply component 13 includes a sheath liquid tank, a sheath liquid sensor and a sheath liquid filter. One end of the sheath liquid filter is connected to the flow chamber 12, and the other end of the sheath liquid filter is connected to the sheath liquid sensor. The sheath liquid tank is connected to the sheath liquid filter through the sheath liquid sensor. Furthermore, when the sheath liquid supply component 13 supplies sheath liquid to the flow chamber 12, the sheath liquid tank can deliver the sheath liquid to the flow chamber 12 through the sheath liquid sensor and the sheath liquid filter. The sheath liquid sensor is used to detect the flow of the sheath liquid to determine whether the sheath liquid delivery pipeline is smooth and ensure the smoothness of the sheath liquid delivery; the sheath liquid filter is used to filter bubbles in the pipeline to prevent bubble blockage from affecting the delivery of the sheath flow.

[0045] In another embodiment, since the power component 15 first extracts part of the first sample liquid to the sample line 14, the first sample liquid on the sample line 14 is pushed to the flow chamber 12 for optical detection after the sheath liquid supply component 13 establishes a stable sheath flow. There is a period of time between the first sample liquid being delivered to the sample line 14 and the first sample liquid being delivered to the flow chamber 12. This embodiment further proposes that after the power component 15 extracts part of the first sample liquid from the reaction pool 11, the liquid transfer component can inject the second reagent into the reaction pool 11 to prepare the second sample liquid, further increasing the overlap time of the preparation time of the second sample liquid and the detection time of the first sample liquid in the sample analyzer 1, reducing the detection time of the sample analyzer 1 to detect the sample to be tested to obtain at least one of red blood cells, platelets, hemoglobin, reticulocytes and immature platelets, and improving the detection efficiency of the sample analyzer 1 to detect the sample to be tested.

[0046] In other embodiments, the sheath liquid supply component 13 supplies sheath liquid to the flow chamber 12 to establish a sheath flow in the flow chamber 12 only after the power component 15 draws the first sample liquid into the sample pipeline 14; or, when the pipetting component injects the sample to be tested and the first reagent into the reaction pool 11, the sheath liquid supply component 13 supplies sheath liquid to the flow chamber 12 to establish a sheath flow in the flow chamber 12, thereby reducing the waiting time for the first sample liquid. The specific action time of the sheath liquid supply component 13 can be determined by the user based on the balance between reagent cost and detection efficiency, and the present application does not impose any restrictions on this.

[0047] Alternatively, if Figure 1 As shown, the sample analyzer 1 further includes a first valve 16 , which is disposed on the sample pipeline 14 .

[0048] After the power assembly 15 draws part of the first sample liquid in the reaction pool 11 into the sample pipeline 14 , the first valve 16 is disconnected, and the sheath liquid supply assembly 13 is used to supply sheath liquid to the flow chamber 12 to establish a sheath flow.

[0049] After the sheath liquid supply assembly 13 forms a stable sheath flow in the flow chamber 12 , the first valve 16 is turned on, and the power assembly 15 pushes the first sample liquid in the sample pipeline 14 into the flow chamber 12 .

[0050] Specifically, the first valve 16 controls the cutoff and conduction of the sample pipeline 14. After the power assembly 15 draws the first sample liquid into the sample pipeline 14, the first valve 16 is disconnected, so that the first sample liquid is stored in the sample pipeline 14 first. The sheath liquid supply assembly 13 supplies sheath liquid to the flow chamber 12 to establish a sheath flow. After the sheath liquid supply assembly 13 forms a stable sheath flow in the flow chamber 12, the first valve 16 is turned on, and the power assembly 15 pushes the first sample in the sample pipeline 14 into the flow chamber 12 for optical detection.

[0051] Optionally, the sheath liquid supply component 13 forms a stable sheath flow in the flow chamber 12 by at least one of the following: the time for the sheath liquid supply component 13 to supply the sheath flow is greater than or equal to a preset time length, and the amount of sheath liquid supplied by the sheath liquid supply component 13 is greater than or equal to a predetermined amount. That is, after the power component 15 extracts part of the first sample liquid in the reaction pool 11 into the sample pipeline 14, the first valve 16 is disconnected, and after the sheath liquid supply component 13 supplies the sheath liquid to the flow chamber 12 for a time length greater than or equal to a preset time length, and / or the amount of sheath liquid supplied by the sheath liquid supply component 13 to the flow chamber 12 is greater than or equal to a preset amount, the first valve 16 is opened again, and then the power component 15 pushes the first sample in the sample pipeline 14 into the flow chamber 12 for optical detection.

[0052] By setting the first valve 16 to maintain the hydraulic pressure in the sample pipeline 14 stable before the sheath flow stabilizes, the dilution degree of the front and rear sections of the first sample liquid in the sample pipeline 14 can be reduced, while avoiding the problem of poor detection consistency caused by different dilution degrees of different sample liquids, thereby improving the detection accuracy of the sample analyzer 1 for the sample to be tested.

[0053] Optionally, the power assembly 15 includes a first power component 151 and a second power component 152. The first power component 151 is connected to one end of the sample line 14 close to the flow chamber 12, and the second power component 152 is connected to one end of the sample line 14 close to the reaction pool 11. The first power component 151 can be a power pump, a syringe, etc., and the second power component 152 can also be a power pump, a syringe, etc.

[0054] The first power component 151 is used to extract part of the first sample liquid or the second sample liquid in the reaction pool 11 into the sample pipeline 14, and make the first end of the first sample liquid or the second sample liquid be located in the pipeline between the flow chamber 12 and the first power component 151. The second power component 152 is used to push the first sample liquid or the second sample liquid in the pipeline between the reaction pool 11 and the flow chamber 12 into the flow chamber 12.

[0055] Specifically, the first power component 151 can apply negative pressure to the sample line 14 so that part of the first sample liquid or the second sample liquid in the reaction pool 11 is extracted into the sample line 14, and the head end of the first sample liquid or the second sample liquid is located in the line between the flow chamber 12 and the first power component 151. In fact, in the process of the first sample liquid or the second sample liquid being extracted into the sample line 14, the front section of the sample flow will be diluted by the diluent originally existing in the sample line 14. In order to ensure the accuracy of the detection, the front section of the sample flow is generally discarded and the middle section of the sample flow is used for detection.

[0056] Therefore, in this embodiment, it is proposed that the first power component 151 draws the head end of the first sample liquid or the second sample liquid into the pipeline between the flow chamber 12 and the first power component 151, and the second power component 152 can apply positive pressure to the sample pipeline 14 to push the first sample liquid or the second sample liquid in the pipeline between the reaction pool 11 and the flow chamber 12 into the flow chamber 12, which can further improve the detection efficiency of the sample.

[0057] In one embodiment, in combination with the first valve 16 described above, the first valve 16 can be disposed on the pipeline between the second power component 152 and the reaction cell 11, or on the pipeline between the reaction cell 11 and the flow chamber 12. After the first power component 151 extracts the first end of the first sample liquid from the pipeline between the flow chamber 12 and the first power component 151, the first valve 16 is cut off, and after the sheath liquid supply assembly 13 establishes a stable sheath flow in the flow chamber 12, the first valve 16 is turned on, and the second power component 152 pushes the first sample liquid in the pipeline between the reaction cell 11 and the flow chamber 12 into the flow chamber 12.

[0058] Since the second sample liquid does not need to wait for the establishment of the sheath flow during detection, after the first power component 151 draws the head end of the second sample liquid into the pipeline between the flow chamber 12 and the first power component 151, the second power component 152 can be activated to push the second sample liquid in the pipeline between the reaction pool 11 and the flow chamber 12 into the flow chamber 12.

[0059] The first power component 151 may also be connected to the sample pipeline 14 via a valve, so that the first power component 151 and the sample pipeline 14 are connected or disconnected.

[0060] Optionally, see Figure 2 , Figure 2 1 is a schematic structural diagram of a second embodiment of a sample analyzer of the present application. The sample pipeline 14 includes a first pipeline 141 and a second pipeline 142 connected in parallel.

[0061] The first pipeline 141 is provided with a first valve 16, and the second pipeline 142 is provided with a second valve 17. The power assembly 15 is used to draw the first sample liquid in the reaction pool 11 into the first pipeline 141 when the first valve 16 is turned on and the second valve 17 is turned off, and the first sample liquid in the first pipeline 141 is pushed into the flow chamber 12; the power assembly 15 is also used to draw the second sample liquid in the reaction pool 11 into the second pipeline 142 when the first valve 16 is turned off and the second valve 17 is turned on, and the second sample liquid in the second pipeline 142 is pushed into the flow chamber 12.

[0062] Specifically, during the detection of the first sample liquid, the first valve 16 can be turned on and the second valve 17 can be turned off, that is, the first pipeline 141 is turned on and the second pipeline 142 is turned off. Then, the power assembly 15 extracts part of the first sample liquid from the reaction pool 11 into the first pipeline 141, and then pushes the first sample liquid in the first pipeline 141 into the flow chamber 12.

[0063] After the first sample liquid is detected, the first valve 16 is cut off and the second valve 17 is opened during the detection of the second sample liquid, that is, the first pipeline 141 is cut off and the second pipeline 142 is opened. Then, the power assembly 15 draws the second sample liquid from the reaction pool 11 into the second pipeline 142, and then pushes the second sample liquid in the second pipeline 142 into the flow chamber 12.

[0064] By respectively setting the first pipeline 141 to transport the first sample liquid and the second pipeline 142 to transport the second sample liquid, the first sample liquid can be prevented from being contaminated. In the process of cleaning the sample pipeline 14, since the power assembly 15 is set at the end of the sample pipeline 14, the cleaning effect of the end of the sample pipeline 14 is better, but the cleaning effect of the middle of the sample pipeline 14 cannot be guaranteed. If the cleaning effect in the sample pipeline 14 is not good after the second sample liquid is transported through the sample pipeline 14, the first sample liquid is transported through the sample pipeline 14 when the second sample liquid remains in the sample pipeline 14. As can be seen from the above, the second sample liquid contains a dye that affects the cell size, and the remaining second sample liquid will contaminate the first sample liquid, affecting the detection result of the first sample liquid. Therefore, by setting the first pipeline 141 and the second pipeline 142, the first sample liquid and the second sample liquid are transported in separate ways, avoiding contamination of the first sample liquid and improving the detection efficiency of the sample analyzer 1.

[0065] At the same time, the first pipeline 141 is set to transport the first sample liquid, and the second pipeline 142 is set to transport the second sample liquid. It can also be achieved that during the detection of the first sample liquid, the incubated second sample liquid can be extracted into the second pipeline 142 for storage, so as to avoid the situation where the second sample liquid is incubated but the detection of the first sample liquid is not completed, the first sample still exists in the sample pipeline 14, and the second sample liquid cannot be extracted from the reaction pool 11, and the second sample liquid continues to incubate in the reaction pool 11, resulting in excessive incubation of the second sample liquid, thereby affecting the detection result of the second sample liquid. By setting the second pipeline 142, this embodiment can achieve that when the first sample liquid exists in the first pipeline 141, the second sample liquid can still be extracted into the second pipeline 142 for storage, thereby improving the efficiency of sample transportation.

[0066] The first valve 16 and the second valve 17 may be valves of the same structural specifications, and this application does not impose any limitation on this.

[0067] Furthermore, the present embodiment also proposes that the length of the pipeline section from the connection between the first pipeline 141 and the reaction pool 11 to the first valve 16 is equal to the length of the pipeline section from the connection between the second pipeline 142 and the reaction pool 11 to the second valve 17; and / or, the length of the pipeline section from the connection between the first pipeline 141 and the flow chamber 12 to the first valve 16 is equal to the length of the pipeline section from the connection between the second pipeline 142 and the flow chamber 12 to the second valve 17; and / or, the length of the pipeline section between the reaction pool 11 and the flow chamber 12 on the first pipeline 141 is equal to the length of the pipeline section between the reaction pool 11 and the flow chamber 12 on the second pipeline 142.

[0068] Among them, as mentioned above, during the process of the sample flow being transported in the pipeline, the diluent in the pipeline will dilute the sample flow. Therefore, this embodiment limits the length of the upper part of the pipeline section of the first pipeline 141 and the second pipeline 142, so that the degree of dilution of the first sample liquid transported in the first pipeline 141 is consistent with the degree of dilution of the second sample liquid transported in the second pipeline 142, thereby ensuring the consistency of the detection of the first sample liquid and the second sample liquid, and improving the detection efficiency of the sample analyzer 1.

[0069] Optionally, see Figure 3 , Figure 3 1 is a schematic diagram of the structure of the third embodiment of the sample analyzer of the present application. The sample analyzer 1 provided in this embodiment further includes a third valve 18 and a liquid storage tube 19 , and the power assembly 15 further includes a third power component 153 .

[0070] The reaction pool 11 is connected to the sample pipeline 14 through the third valve 18 , one end of the liquid storage tube 19 is connected to the reaction pool 11 through the third valve 18 , and the third power component 153 is connected to one end of the liquid storage tube 19 .

[0071] After the first sample liquid is formed in the reaction pool 11 , the third valve 18 is switched to connect the liquid storage tube 19 to the reaction pool 11 , and the third power component 153 extracts part of the first sample liquid in the reaction pool 11 into the liquid storage tube 19 .

[0072] Specifically, after the first sample liquid is formed in the reaction pool 11, the third valve 18 is switched to connect the reaction pool 11 with the liquid storage tube 19, and then the third power component 153 extracts part of the first sample liquid into the liquid storage tube 19, wherein the reagent amount of the first sample liquid stored in the liquid storage tube 19 can be the reagent amount required to form the second sample liquid, so as to ensure the amount of the sample to be tested in the second sample liquid and ensure the detection efficiency of the second sample liquid.

[0073] In one embodiment, the third power component 153 may be a quantitative pump to draw a quantitative amount of the first sample liquid into the liquid storage tube 19 for storage.

[0074] In fact, as mentioned above, the second sample liquid is a mixture of the first sample liquid and the second reagent. In order to ensure the detection efficiency of the second sample liquid, the reagent amount of the first sample liquid in the second sample liquid needs to be ensured. If the reagent amount of the first sample liquid required by the second sample liquid is not removed first, when the first power component 151 draws the first sample liquid into the sample pipeline 14, the reagent amount of the first sample liquid drawn cannot be determined, and the reagent amount of the first sample liquid remaining in the reaction pool 11 cannot be determined, which may affect the reagent amount of the first sample liquid in the second sample liquid.

[0075] For example, if the reagent amount of the first sample liquid extracted by the first power component 151 is too much, the reagent amount of the first sample liquid remaining in the reaction pool 11 is less, and the amount of the first sample liquid contained in the formed second sample liquid is too little, the amount of cells is insufficient, and the second reagent will be relatively more, which may cause excessive processing of the sample, damage the cells, and affect the detection efficiency of the second sample liquid. Or if the reagent amount of the first sample liquid extracted by the first power component 151 is less, the reagent amount of the first sample liquid remaining in the reaction pool 11 is more, and the second reagent will be relatively less, which may cause insufficient processing of the second sample liquid, and also affect the detection efficiency of the second sample liquid.

[0076] Based on the above analysis, this embodiment proposes to use the cooperation of the liquid storage tube 19 and the third power component 153, so that before the first power component 151 extracts the first sample liquid, the reagent amount of the first sample liquid required by the second sample liquid is extracted into the liquid storage tube 19 for storage to ensure the stability of the reagent amount.

[0077] Furthermore, after the first sample liquid is poured into the liquid storage tube 19, the third valve 18 can be switched to connect the reaction pool 11 with the sample pipeline 14, and the first power component 151 extracts the remaining first sample liquid in the reaction pool 11 into the sample pipeline 14, so that the first sample liquid can be optically detected. The third valve 18 is then switched for the second time to connect the reaction pool 11 with the liquid storage tube 19, and the third power component 153 pushes the first sample liquid in the liquid storage tube 19 into the reaction pool 11, and then the liquid transfer assembly can inject the second reagent into the reaction pool 11 to form a second sample liquid.

[0078] It should be noted that the first power component 151 completely evacuates the remaining first sample liquid in the reaction pool 11 to further ensure that the reagent amount of the first sample liquid involved in forming the second sample liquid is only the reagent amount of the first sample liquid stored in the liquid storage tube 19 .

[0079] In one embodiment, after the first power component 151 extracts the remaining first sample liquid in the reaction pool 11 into the sample pipeline 14, the third valve 18 is immediately switched to the liquid storage tube 19 connected to the reaction pool 11, or the third valve 18 is switched to the liquid storage tube 19 connected to the reaction pool 11 during or after the first sample liquid detection. The user can set it according to needs, and this application does not impose any restrictions on this.

[0080] In this embodiment, a liquid storage tube 19 is provided in the sample analyzer 1 to store part of the first sample liquid, thereby ensuring the reagent amount of the first sample liquid in the second sample liquid, thereby ensuring the detection efficiency of the second sample liquid, improving the detection efficiency and detection accuracy of the sample analyzer 1 for the first sample liquid and the second sample liquid, and improving the user experience of the sample analyzer 1.

[0081] Optionally, the first valve 16 is disposed in the pipeline section between the reaction pool 11 and the flow chamber 12, and the sample analyzer 1 further includes a diluent supply assembly (not shown), which is connected to one end of the sample pipeline 14 close to the reaction pool 11, so that when at least one of the sample pipeline 14, the reaction pool 11 and the flow chamber 12 needs to be cleaned, the diluent supply assembly supplies the diluent through the second power component to perform the cleaning operation. In one embodiment, the diluent supply assembly can be connected to the second power component 152 to supply the diluent to the sample pipeline 14 through the second power component 152.

[0082] The diluent supply assembly is used to supply diluent to the reaction pool 11 when the first valve 16 is cut off and the sheath liquid supply assembly 13 supplies sheath liquid to the flow chamber 12, so as to clean the reaction pool 11. The diluent supply assembly is also used to clean the reaction pool 11, the sample pipeline 14 and the flow chamber 12 when the first valve 16 is turned on.

[0083] Specifically, since the first valve 16 is arranged in the pipeline between the reaction pool 11 and the flow chamber 12, and the diluent supply assembly is connected to one end of the sample pipeline 14 close to the reaction pool 11, when the first valve 16 is cut off, that is, the pipeline between the reaction pool 11 and the flow chamber 12 is cut off, the diluent supply assembly can supply diluent to the reaction pool 11 alone to clean the reaction pool 11 without affecting the flow chamber 12 and the sheath liquid supply assembly 13, and will not affect the process of the sheath liquid supply assembly 13 establishing the sheath flow in the flow chamber 12.

[0084] When the first valve 16 is turned on, that is, the pipeline between the reaction pool 11 and the flow chamber 12 is turned on, the diluent supply component can supply diluent to the reaction pool 11, the sample pipeline 14 and the flow chamber 12 to clean the reaction pool 11, the sample pipeline 14 and the flow chamber 12 to avoid contamination of subsequent samples and improve the detection efficiency of the sample analyzer 1.

[0085] In another embodiment, after the first sample liquid is detected, the first valve 16 may be turned on and the third valve 18 may be turned off, and the diluent supply assembly may supply diluent to the sample line 14 and the flow chamber 12 to clean the sample line 14 and the flow chamber 12, thereby improving the detection efficiency of the second sample liquid.

[0086] In summary, the sample analyzer 1 provided in the embodiment of the present application adopts the method of adding the first reagent and the second reagent to the reaction pool 11 in a time-sharing manner, and can detect at least one of the red blood cells, platelets, hemoglobin, reticulocytes and immature platelets in the sample to be tested, without the need to separately detect different cells in the sample to be tested, thereby improving the efficiency of cell detection by the sample analyzer 1. At the same time, by setting the method of adding the first reagent to the sample to be tested to form a first sample liquid, and then testing the first sample liquid before adding the second reagent to the reaction pool 11, it is avoided that the added second reagent will affect the detection results of red blood cells, platelets and hemoglobin, and the order of adding different reagents is reasonably planned, thereby further improving the detection accuracy of the sample analyzer 1 for the sample to be tested.

[0087] The present application also provides a sample detection method, which is applied to the sample analyzer 1 as described above, comprising: injecting a sample to be tested and a first reagent into a reaction pool 11 to form a first sample liquid, and transporting the first sample liquid to an optical detection component for optical detection to obtain a detection result of at least one of red blood cells, platelets and hemoglobin in the sample to be tested; during the detection of the first sample liquid, injecting a second reagent into the reaction pool 11 to form a second sample liquid, and after the detection of the first sample liquid is completed, transporting the second sample liquid to the optical detection component for optical detection to obtain a detection result of at least one of reticulocytes and immature platelets in the sample to be tested.

[0088] The following is a brief description of the sample detection method process: In some embodiments, the pipetting component injects the sample to be tested and the first reagent into the reaction pool 11, the mixing component and the incubation component mix and incubate the sample to be tested and the first reagent to form a first sample liquid. After the first sample liquid is formed, the power component 15 draws the first sample liquid into the sample line 14, and then pushes the first sample liquid in the sample line 14 into the flow chamber 12 for optical detection to obtain a detection result of at least one of red blood cells, platelets and hemoglobin in the sample to be tested.

[0089] During the detection of the first sample liquid, the pipetting component injects the second reagent into the reaction pool 11 to form a second sample liquid in the reaction pool 11. After the detection of the first sample liquid is completed and the sample pipeline 14 and the flow chamber 12 are cleaned, the power component 15 extracts the second sample liquid in the reaction pool 11 into the sample pipeline 14, and then pushes the second sample liquid in the sample pipeline 14 into the flow chamber 12, so that the optical detection component performs optical detection on the second sample to obtain the detection result of at least one of the reticulocytes and immature platelets in the sample to be tested.

[0090] By adding the first reagent and the second reagent to the reaction pool 11 at different times, the second reagent is added to the reaction pool 11 during the detection of the first sample liquid, thereby avoiding the added second reagent from affecting the detection results of red blood cells, platelets and hemoglobin. By rationally planning the order of adding different reagents, the red blood cells, platelets, hemoglobin, reticulocytes and immature platelets in the sample to be tested can be detected at one time, without the need to separately detect different cells in the sample to be tested, thereby improving the detection efficiency and detection accuracy of the sample to be tested.

[0091] In some embodiments, after the power assembly 15 draws the first sample liquid into the sample line 14, the first valve 16 is disconnected to stabilize the hydraulic pressure of the sample line 14, so that the first sample liquid is temporarily stored in the sample line 14, and the sheath liquid supply assembly 13 establishes a sheath flow in the flow chamber 12. After the sheath liquid supply assembly 13 forms a stable sheath flow in the flow chamber 12, the first valve 16 is opened, and the power assembly 15 transports the first sample liquid in the sample line 14 to the flow chamber 12 for optical detection, thereby improving the detection efficiency of the first sample liquid.

[0092] As described above, after the sheath liquid supply assembly 13 forms a stable sheath flow in the flow chamber 12, the sheath liquid supply assembly 13 is always open. Therefore, after the power assembly 15 draws the second sample liquid into the sample pipeline 14, the first valve 16 does not need to be operated, and the power assembly 15 can immediately push the second sample liquid into the flow chamber 12.

[0093] Specifically, after the first sample liquid or the second sample liquid is formed in the reaction pool 11 , the first power component 151 draws the first sample liquid or the second sample liquid into the sample line 14 , and then the second power component 152 pushes the first sample liquid or the second sample liquid in the sample line 14 into the flow chamber 12 .

[0094] When the first power component 151 extracts the first sample liquid or the second sample liquid, the first power component 151 may draw the head end of the first sample liquid or the second sample liquid into the pipeline between the flow chamber 12 and the first power component 151, so as to avoid affecting the detection result due to the head end of the first sample liquid or the second sample liquid being diluted by the diluent in the sample pipeline 14, thereby improving the detection efficiency of the first sample liquid and the second sample liquid.

[0095] In some embodiments, the power assembly 15 transports the first sample liquid through the first pipeline 141, and transports the second sample liquid through the second pipeline 142. Specifically, the first valve 16 on the first pipeline 141 is turned on, and the second valve 17 on the second pipeline 142 is turned off. Then the power assembly 15 can transport the first sample liquid in the reaction pool 11 through the first pipeline 141, so that the first sample liquid is transported to the flow chamber 12 through the first pipeline 141 for optical detection. Then, the first valve 16 is turned off, and the second valve 17 is turned on. Then the power assembly 15 can transport the second sample liquid in the reaction pool 11 through the second pipeline 142, so that the second sample liquid is transported to the flow chamber 12 through the second pipeline 142 for optical detection.

[0096] The first sample liquid and the second sample liquid are transported respectively through the first pipeline 141 and the second pipeline 142 connected in parallel, thereby reducing the contamination of the sample liquid and improving the detection efficiency of the first sample liquid and the second sample liquid.

[0097] Among them, the length of the pipeline section from the connection between the first pipeline 141 and the reaction pool 11 to the first valve 16 can be equal to the length of the pipeline section from the connection between the second pipeline 142 and the reaction pool 11 to the second valve 17; and / or, the length of the pipeline section from the connection between the first pipeline 141 and the flow chamber 12 to the first valve 16 can be equal to the length of the pipeline section from the connection between the second pipeline 142 and the flow chamber 12 to the second valve 17; and / or, the length of the pipeline section between the reaction pool 11 and the flow chamber 12 on the first pipeline 141 can be equal to the length of the pipeline section between the reaction pool 11 and the flow chamber 12 on the second pipeline 142. The dilution degree of the first sample liquid in the first pipeline 141 is consistent with the dilution degree of the second sample liquid in the second pipeline 142, which ensures the consistency of the detection of the first sample liquid and the second sample liquid, and improves the detection efficiency of the sample analyzer 1.

[0098] In some embodiments, after the first sample liquid is formed in the reaction pool 11, the third valve 18 is switched to the liquid storage tube 19 to communicate with the reaction pool 11, and then the third power component 153 can extract the reagent amount of the first sample liquid required for the second sample liquid into the liquid storage tube 19 for storage, and then the third valve 18 is switched to the reaction pool 11 to communicate with the sample pipeline 14, and the first power component 151 is activated to extract the remaining first sample liquid in the reaction pool 11 into the sample pipeline 14. After the remaining first sample liquid in the reaction pool 11 is extracted, the third valve 18 can be switched again to the liquid storage tube 19 to communicate with the reaction pool 11, and the third power component 153 pushes the first sample liquid in the liquid storage tube 19 into the reaction pool 11 to participate in the subsequent second sample liquid formation step.

[0099] By first storing the reagent amount of the first sample liquid required for the second sample liquid, the content of the first sample liquid in the prepared second sample liquid can be guaranteed, thereby improving the detection efficiency of the second sample liquid.

[0100] In addition, based on the above embodiment, the third valve 18 may be cut off, and the diluent supply assembly may supply diluent to the sample line 14 and the flow chamber 12 to clean the sample line 14 and the flow chamber 12 without affecting the liquid in the reaction pool 11 .

[0101] In some embodiments, the first valve 16 may be disposed between the flow chamber 12 and the reaction cell 11, and when the first valve 16 is cut off, the diluent supply assembly may only supply the diluent to the reaction cell 11 to clean the reaction cell 11 without affecting the flow chamber 12 and the sheath flow in the flow chamber 12. When the first valve 16 is turned on, the diluent supply assembly may supply the diluent to the reaction cell 11, the sample line 14, and the flow chamber 12 to clean the reaction cell 11, the sample line 14, and the flow chamber 12.

[0102] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A sample analyzer, characterized in that: It includes a reaction pool, a pipetting component, an optical detection component and a sheath liquid supply component, wherein the pipetting component and the reaction pool are spaced apart; the reaction pool is connected to a flow chamber of the optical detection component, and the sheath liquid supply component is connected to the flow chamber; Wherein, the pipetting component is used to inject the sample to be tested and the first reagent into the reaction pool to form a first sample liquid; The sheath liquid supply component is used to supply sheath liquid to the flow chamber of the optical detection component to form a sheath flow in the flow chamber; The optical detection component is used to perform optical detection on a portion of the first sample liquid to obtain a detection result of at least one of red blood cells, platelets and hemoglobin in the sample to be tested; The liquid transfer assembly is also used to inject a second reagent into the reaction pool so that the remaining first sample liquid and the second reagent form a second sample liquid; The optical detection component is also used to perform optical detection on the second sample liquid to obtain a detection result of at least one of reticulocytes and immature platelets in the sample to be tested.

2. The sample analyzer according to claim 1, characterized in that: The sample analyzer also includes a sample pipeline connecting the reaction pool and the flow chamber, and a power component connected to the sample pipeline; The power component is used to extract part of the first sample liquid in the reaction pool into the sample pipeline, and after the sheath liquid supply component forms a stable sheath flow in the flow chamber, push the first sample liquid in the sample pipeline into the flow chamber; The power assembly is also used to push the second sample liquid in the reaction pool to the flow chamber via the sample pipeline after the first sample liquid is detected and the sample pipeline is cleaned.

3. The sample analyzer according to claim 2, characterized in that: The sample analyzer further comprises a first valve, which is disposed on the sample pipeline; After the power assembly draws part of the first sample liquid in the reaction pool into the sample pipeline, the first valve is disconnected, and the sheath liquid supply assembly is used to supply sheath liquid to the flow chamber to establish the sheath flow; After the sheath liquid supply assembly forms a stable sheath flow in the flow chamber, the first valve is turned on, and the power assembly pushes the first sample liquid in the sample pipeline into the flow chamber.

4. The sample analyzer according to claim 2, characterized in that: The power assembly includes a first power component and a second power component; the first power component is connected to one end of the sample pipeline close to the flow chamber, and the second power component is connected to one end of the sample pipeline close to the reaction pool; The first power component is used to extract part of the first sample liquid or the second sample liquid in the reaction pool into the sample pipeline, and make the first end of the first sample liquid or the second sample liquid be located in the pipeline between the flow chamber and the first power component; The second power component is used to push the first sample liquid or the second sample liquid in the pipeline between the reaction pool and the flow chamber into the flow chamber.

5. The sample analyzer according to claim 3, characterized in that: The sample pipeline includes a first pipeline and a second pipeline connected in parallel; The first pipeline is provided with the first valve, and the second pipeline is provided with the second valve; The power assembly is used to draw part of the first sample liquid in the reaction pool into the first pipeline when the first valve is turned on and the second valve is turned off, and push the first sample liquid in the first pipeline into the flow chamber; The power assembly is also used to draw the second sample liquid in the reaction pool into the second pipeline when the first valve is cut off and the second valve is turned on, and push the second sample liquid in the second pipeline into the flow chamber.

6. The sample analyzer according to claim 5, characterized in that: The length of the pipeline section from the connection between the first pipeline and the reaction tank to the first valve is equal to the length of the pipeline section from the connection between the second pipeline and the reaction tank to the second valve; and / or, the length of the pipeline section from the connection point between the first pipeline and the flow chamber to the first valve is equal to the length of the pipeline section from the connection point between the second pipeline and the flow chamber to the second valve; And / or, the length of the pipeline section between the reaction pool and the flow chamber on the first pipeline is equal to the length of the pipeline section between the reaction pool and the flow chamber on the second pipeline.

7. The sample analyzer according to claim 4, characterized in that: The sample analyzer further includes a liquid storage tube and a third valve, and the power assembly further includes a third power component. The reaction pool is connected to the sample pipeline through the third valve, one end of the liquid storage tube is connected to the reaction pool through the third valve, and the third power component is connected to the other end of the liquid storage tube; After the first sample liquid is formed in the reaction pool, the third valve is switched to connect the liquid storage tube and the reaction pool, and the third power component is used to extract part of the first sample liquid in the reaction pool into the liquid storage tube.

8. The sample analyzer according to claim 7, characterized in that: After the first sample liquid is poured into the liquid storage tube, the third valve is switched to connect the reaction pool and the sample pipeline, and the first power component is used to extract the remaining first sample liquid in the reaction pool into the sample pipeline; The third valve is switched for the second time so that the reaction pool is connected to the liquid storage tube, and the third power component is also used to push the first sample liquid in the liquid storage tube into the reaction pool.

9. The sample analyzer according to claim 3, characterized in that: The first valve is disposed in the pipeline section between the reaction pool and the flow chamber, and the sample analyzer further comprises a diluent supply assembly, and the diluent supply assembly is connected to one end of the sample pipeline close to the reaction pool; The diluent supply component is used to supply diluent to the reaction pool to clean the reaction pool when the first valve is cut off and the sheath liquid supply component supplies sheath liquid to the flow chamber; The diluent supply assembly is also used to supply the diluent to the reaction pool, the sample pipeline and the flow chamber when the first valve is turned on, so as to clean the reaction pool, the sample pipeline and the flow chamber.

10. A sample detection method, characterized in that: A sample analyzer as claimed in any one of claims 1 to 9, comprising: Injecting the sample to be tested and the first reagent into the reaction pool to form a first sample solution, and transporting the first sample solution to the optical detection component for optical detection to obtain a detection result of at least one of red blood cells, platelets and hemoglobin in the sample to be tested; During the detection of the first sample liquid, a second reagent is injected into the reaction pool to form a second sample liquid, and after the detection of the first sample liquid is completed, the second sample liquid is transported to the optical detection component for optical detection to obtain a detection result of at least one of reticulocytes and immature platelets in the sample to be tested.

Images