Composition containing umbilical cord collagen active peptide and preparation method thereof
By preparing a composition containing collagen-active peptide of umbilical cord, combined with medicinal materials such as Atractylodes, Asparagus, Achyranthes ulmoides, Eucommia ulmoides, musk, etc., the research problem of lacking compound anti-inflammatory drugs in the prior art was solved, and the goal of enhancing anti-inflammatory effects and improving system stability was achieved.
Patent Information
- Application Number
- CN202510234231.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2025-05-13
AI Technical Summary
There is a lack of research on compound anti-inflammatory drugs containing umbilical cord collagen active peptides in the prior art, and the preparation method of such compositions has not been fully explored.
A composition containing collagen-active peptide of umbilical cord is provided and a preparation method thereof, which is prepared by mixing Atractylodes, Asparagus, Achyranthes ulmoides, Eucommia ulmoides, Musk and Umbilical cord collagen-active peptides in a specific proportion, and are prepared by 85% ethanol immersion, extraction of low-temperature vibration mill, and rotary evaporator with reduced pressure distillation.
A unique anti-inflammatory system has been formed, with the ability to enhance anti-inflammatory effects, improve system stability and improve drug efficacy.
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Abstract
Description
Technical Field
[0001] The present application relates to the technical field of anti-inflammatory drugs, and in particular to a composition containing umbilical cord collagen active peptides and a preparation method thereof. Background Art
[0002] The umbilical cord is mainly composed of collagen and elastic fibers except blood vessels. The umbilical cord collagen active peptide of the present application is often called umbilical cord peptide, which is extracted from animal umbilical cord collagen by enzymatic hydrolysis.
[0003] Collagen has anti-inflammatory and pro-repair properties. Referring to human cells, it is warm in nature and can enter the lung and kidney meridians. Shi Yuanyuan et al.'s "Research Progress on the Effect of Collagen Sponge on the Level of Inflammatory Factors in Burn Wounds" records that "collagen sponges inhibit inflammatory cytokines, increase the thickness of granulation tissue, promote the formation of epithelial cells and reduce the number of inflammatory cells, thereby healing wounds in a faster manner and helping to reduce the quality of wound repair"; the umbilical cord collagen active peptide of this application, as a type of collagen peptide, combined with the umbilical cord anti-inflammatory record of Li Wenxin et al.'s "Umbilical Cord Decoction is Effective in Treating Intractable Colds and Enteritis in Infants", has research prospects as an anti-inflammatory drug.
[0004] However, there are few references to compound anti-inflammatory compositions containing umbilical cord collagen active peptides. Research on compound anti-inflammatory drugs combined with umbilical cord collagen active peptides obviously has great prospects; therefore, there is a need for a composition containing umbilical cord collagen active peptides and a preparation method thereof. Summary of the invention
[0005] In order to solve the above problems, the present invention aims to provide a composition containing umbilical cord collagen active peptides and a preparation method thereof.
[0006] On the one hand, the present application provides a method for preparing a composition containing umbilical cord collagen active peptides, the method for preparing the composition containing umbilical cord collagen active peptides comprising the following steps: Step 1: Take 25-36% by mass of Atractylodes lancea, 19-25% by mass of Asparagus cochinchinensis, 10-15% by mass of Achyranthes bidentata, 6-10% by mass of Eucommia ulmoides and make up the rest by mass of pure water, chop the above medicinal materials and pass through a 20-mesh sieve, add 85% ethanol with a mass of 6 times the total mass of the above medicinal materials and water, immerse at 13°C for 24h, pour the solid and liquid into a low-temperature vibration mill together, extract at -5°C for 6min, and filter at 1500 mesh to obtain the extract after discharge; Step 2: Add 0.3 times the mass of pure water to the extract, and then perform reduced pressure distillation in a rotary evaporator at 60° C. and a pressure of 150 mbar until no liquid continuously flows out, collect the remaining liquid, add umbilical cord collagen active peptides with a mass fraction of 13-22% of the mass of the remaining liquid and musk with a mass fraction of 2-4% of the mass of the remaining liquid, and mix them to obtain the composition containing umbilical cord collagen active peptides.
[0007] Furthermore, the mass fraction of the Atractylodes lancea is 30%.
[0008] Furthermore, the mass fraction of the asparagus is 22%.
[0009] Furthermore, the mass fraction of the Achyranthes bidentata is 13%.
[0010] Furthermore, the mass fraction of the Eucommia ulmoides is 8%.
[0011] Furthermore, the mass fraction of the umbilical cord collagen active peptide is 18%.
[0012] Furthermore, the mass fraction of the musk is 3%.
[0013] On the other hand, the present application provides a composition containing umbilical cord collagen active peptides prepared by the preparation method of the composition containing umbilical cord collagen active peptides as described above.
[0014] This application can bring the following beneficial effects: The composition containing umbilical cord collagen active peptide of the present application has a unique anti-inflammatory system: Atractylodes lancea dries dampness and strengthens the spleen and dispels wind and cold, has anti-inflammatory and immunomodulatory effects, and can be combined with umbilical cord collagen active peptide to enhance the anti-inflammatory effect. Further, Atractylodes lancea oil has a physical wrapping and preservation effect on each component, thereby improving the stability of the system; Asparagus cochinchinensis and umbilical cord collagen active peptide enter the lung and kidney meridians together, and the cold nature of Asparagus cochinchinensis can balance the warm nature of the umbilical cord collagen active peptide. At the same time, the asparagus polysaccharide contained in Asparagus cochinchinensis has the effect of scavenging free radicals and resisting lipid peroxidation, which is beneficial to maintaining the activity of each component under the process of the present application and improving the stability of the system; Achyranthes bidentata dredges the meridians, and is helpful for the drug to take effect when combined with the components of the present application; Eucommia ulmoides replenishes the middle and benefits the essence and can improve immunity, and can be combined with umbilical cord collagen active peptide to enhance the anti-inflammatory effect; Musk is warm in nature, and on the one hand, it is helpful for the drug to take effect when it moves around and opens the mind, and on the other hand, it has a fragrant smell that improves the taste when taken; Umbilical cord collagen active peptide can be combined with the components and processes of the present application to enhance the anti-inflammatory effect.
[0015] The above-mentioned components of the present application, under the unique process preparation and proportion of the present application, together form the anti-inflammatory oral drug system of the present application, including the synergy of drug efficacy and the coordination of system stability. It should be noted that the process parameters and drug proportions of the present application are not obtained by simple experiments, and have certain randomness and creativity. The proportions of the components cannot only consider their respective efficacy, but also have an impact on the overall stability. DETAILED DESCRIPTION
[0016] In order to more clearly explain the overall concept of the present application, the overall scheme of the present invention is described in detail in the form of an embodiment below; in the following description, a large number of specific details are given to provide a more thorough understanding of the present invention; however, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details; in other examples, in order to avoid confusion with the present invention, some technical features known in the art are not described.
[0017] In this application, the low-temperature vibration mill is Leimai ZD-10L, with a power of 1.5kW and a processing capacity of 5kg per feeding; the rotary evaporator is purchased from Qingdao Juchuang Environmental Protection Group Co., Ltd., model JC-ZFA-2L, two liters per batch; the water content of Atractylodes, Asparagus, Achyranthes, Eucommia and Musk is 10%, all purchased from Anhui Kangweifu Pharmaceutical Co., Ltd., and Achyranthes is specifically Sichuan Achyranthes; Umbilical cord collagen active peptide is purchased from Shaanxi Tiannong Biotechnology Co., Ltd., made from sheep umbilical cord, item number TN9148-7. The above-mentioned Chinese medicines all meet the standards of the State Food and Drug Administration's "Quality Management Standards for the Production of Chinese Medicinal Materials" and the "Chinese Pharmacopoeia".
[0018] The operation of white blood cell counting is as follows: prepare white blood cell diluent: 2mL glacial acetic acid, 98mL distilled water, 3 drops of 10g / L methylene blue solution, mix and filter for use; dilute blood: add 0.38mL white blood cell diluent to the test tube, accurately draw 20μL of blood sample with a pipette, then wipe the remaining blood along the pipette mouth with cotton, and check whether the blood volume is sufficient; insert the pipette into the bottom of the diluent in the small test tube, slowly drain the blood in the pipette, then draw the supernatant to wash the pipette 3 times, mix well, and make a white blood cell suspension; Flushing: Let it stand for a while until the red blood cells are completely destroyed, then mix again; draw 10μL and flush it into the counting pool along the gap between the cover glass and the counting plate; the counting pool should be full. If there are too much, too little or bubbles, you need to flush it again; counting: let it stand for 2-3 minutes after flushing, and after the white blood cells have completely sunk, use a low-power microscope to count the number of white blood cells in the 4 large squares in the four corners. Under the microscope, the white blood cells are round, with clear cytoplasm and darkly stained nuclei. For the white blood cells on the line, the principle of "record the upper ones but not the lower ones, count the left ones but not the right ones" is adopted.
[0019] Unless otherwise specified, all raw material components in the following examples can be purchased from commercial sources, the experimental instruments used are conventional laboratory experimental instruments, and the performance test methods are known in the art. The overall space environment is 25°C and the air humidity is 30%.
[0020] The preferred implementation is as follows: Embodiment 1: The preparation method of the composition containing umbilical cord collagen active peptide is as follows: Step 1: Take 30% by mass of Atractylodes lancea, 22% by mass of Asparagus cochinchinensis, 13% by mass of Achyranthes bidentata, 8% by mass of Eucommia ulmoides and make up the rest by mass of pure water, chop the above medicinal materials and pass through a 20-mesh sieve, add 85% ethanol with a mass of 6 times the total mass of the above medicinal materials and water, immerse at 13°C for 24 hours, pour the solid and liquid into a low-temperature vibration mill together, extract at -5°C for 6 minutes, and filter through 1500 mesh to obtain the extract after discharging; Step 2: Add 0.3 times the mass of pure water to the extract and perform reduced pressure distillation in a rotary evaporator at 60°C and a pressure of 150 mbar until no liquid flows out continuously, collect the remaining liquid, add 18% by mass of umbilical cord collagen active peptides and 3% by mass of musk to mix to obtain the composition containing umbilical cord collagen active peptides.
[0021] The composition containing the umbilical cord collagen active peptide is prepared by adopting the preparation method of the composition containing the umbilical cord collagen active peptide as mentioned above.
[0022] Embodiment 2-13: The only difference between Example 2 and Example 1 is that the mass fraction of Atractylodes lancea is 25%; The only difference between Example 3 and Example 1 is that the mass fraction of Atractylodes lancea is 36%; The only difference between Example 4 and Example 1 is that the mass fraction of asparagus is 19%; The only difference between Example 5 and Example 1 is that the mass fraction of asparagus is 25%; The only difference between Example 6 and Example 1 is that the mass fraction of Achyranthes bidentata is 10%; The only difference between Example 7 and Example 1 is that the mass fraction of Achyranthes bidentata is 15%; The only difference between Example 8 and Example 1 is that the mass fraction of Eucommia ulmoides is 6%; The only difference between Example 9 and Example 1 is that the mass fraction of Eucommia ulmoides is 10%; The only difference between Example 10 and Example 1 is that the mass fraction of the umbilical cord collagen active peptide is 13%; The only difference between Example 11 and Example 1 is that the mass fraction of the umbilical cord collagen active peptide is 22%; The only difference between Example 12 and Example 1 is that the mass fraction of musk is 2%; The only difference between Example 13 and Example 1 is that the mass fraction of musk is 4%.
[0023] Comparative Examples 1-20: The difference between Comparative Example 1 and Example 1 is that the mass fraction of Atractylodes lancea is 10%; The difference between Comparative Example 2 and Example 1 is that the mass fraction of Atractylodes lancea is 50%; The only difference between Comparative Example 3 and Example 1 is that the mass fraction of Asparagus cochinchinensis is 10%; The only difference between Comparative Example 4 and Example 1 is that the mass fraction of Asparagus cochinchinensis is 40%; The difference between Comparative Example 5 and Example 1 is that the mass fraction of Achyranthes bidentata is 5%; The only difference between Comparative Example 6 and Example 1 is that the mass fraction of Achyranthes bidentata is 30%; The only difference between Comparative Example 7 and Example 1 is that the mass fraction of Eucommia ulmoides is 3%; The difference between Comparative Example 8 and Example 1 is that the mass fraction of Eucommia ulmoides is 20%; The only difference between Comparative Example 9 and Example 1 is that the mass fraction of umbilical cord collagen active peptide is 5%; The only difference between Comparative Example 10 and Example 1 is that the mass fraction of umbilical cord collagen active peptide is 35%; The only difference between Comparative Example 11 and Example 1 is that the mass fraction of musk is 1%; The only difference between Comparative Example 12 and Example 1 is that the mass fraction of musk is 8%; The only difference between Comparative Example 13 and Example 1 is that the low-temperature vibration mill extraction temperature is 25°C; The difference between Comparative Example 14 and Example 1 is that Atractylodes lancea is replaced with Asparagus cochinchinensis, Achyranthes bidentata and Eucommia ulmoides in the same mass ratio as in Example 1; The difference between Comparative Example 15 and Example 1 is that Asparagus cochinchinensis is replaced with Atractylodes lancea, Achyranthes bidentata and Eucommia ulmoides in the same mass ratio as in Example 1; The difference between Comparative Example 16 and Example 1 is that Achyranthes bidentata is replaced by Atractylodes lancea, Asparagus cochinchinensis and Eucommia ulmoides in the same mass ratio as in Example 1; The difference between Comparative Example 17 and Example 1 is that Eucommia ulmoides is replaced by Atractylodes lancea, Asparagus cochinchinensis and Achyranthes bidentata in the same mass ratio as in Example 1; The difference between Comparative Example 18 and Example 1 is that the umbilical cord collagen active peptide is replaced with musk of equal mass; The difference between Comparative Example 19 and Example 1 is that the musk is replaced with an equal mass of umbilical cord collagen active peptide; The only difference between Comparative Example 20 and Example 1 is that the composition containing umbilical cord collagen active peptides is replaced with an equal mass of pure water.
[0024] This experiment uses the change in white blood cell count to indicate the anti-inflammatory effect: In cooperation with the National Clinical Research Volunteer Database, 35-year-old male volunteers were recruited for this application. Their inflammatory conditions were basically the same before this experiment, and the white blood cell count was 16.9×10 9 / L, difference ±1×10 8 / L, no hemolysis; each volunteer corresponds to and only corresponds to one example of each experiment; each volunteer orally takes 15g of the composition obtained in each example every day, and is prohibited from taking other drugs during the period. After 3 days, the hospital is commissioned to test the blood for white blood cell count, and the unit of white blood cell count is 10 9 The results of each test are shown in Table 1.
[0025] Table 1: Test results of white blood cell count of volunteers after taking the medicine for 3 days
[0026] It can be seen from the data in Table 1 that, compared with other examples, the composition containing umbilical cord collagen active peptides prepared by the embodiments of the present application, especially the composition containing umbilical cord collagen active peptides and the preparation method thereof of Example 1 of the present application, has a smaller white blood cell count under the same usage amount and usage method; that is, the composition containing umbilical cord collagen active peptides obtained by the present application, especially the scheme of Example 1 of the present application, has a better anti-inflammatory effect.
[0027] It should be noted that the above process parameters and effects are nonlinearly correlated, and the matching conditions with other components and processes of the present application should be considered. The above description is only an embodiment of the present application and is not intended to limit the present application; for those skilled in the art, the present application may have various changes and variations; any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present application shall be included in the scope of the claims of the present application.
Claims
1. A method for preparing a composition containing umbilical cord collagen active peptides, characterized in that: The preparation method of the composition containing umbilical cord collagen active peptides comprises the following steps: Step 1: Take 25-36% by mass of Atractylodes lancea, 19-25% by mass of Asparagus cochinchinensis, 10-15% by mass of Achyranthes bidentata, 6-10% by mass of Eucommia ulmoides and make up the rest by mass of pure water, chop the above medicinal materials and pass through a 20-mesh sieve, add 85% ethanol with a mass of 6 times the total mass of the above medicinal materials and water, immerse at 13°C for 24h, pour the solid and liquid into a low-temperature vibration mill together, extract at -5°C for 6min, and filter at 1500 mesh to obtain the extract after discharge; Step 2: Add 0.3 times the mass of pure water to the extract, and then perform reduced pressure distillation in a rotary evaporator at 60° C. and a pressure of 150 mbar until no liquid continuously flows out, collect the remaining liquid, add umbilical cord collagen active peptides with a mass fraction of 13-22% of the mass of the remaining liquid and musk with a mass fraction of 2-4% of the mass of the remaining liquid, and mix them to obtain the composition containing umbilical cord collagen active peptides.
2. The method for preparing the composition containing umbilical cord collagen active peptide according to claim 1, characterized in that: The mass fraction of the Atractylodes lancea is 30%.
3. The method for preparing the composition containing umbilical cord collagen active peptide according to claim 1, characterized in that: The mass fraction of the asparagus is 22%.
4. The method for preparing the composition containing umbilical cord collagen active peptide according to claim 1, characterized in that: The mass fraction of the Achyranthes bidentata is 13%.
5. The method for preparing the composition containing umbilical cord collagen active peptide according to claim 1, characterized in that: The mass fraction of the Eucommia ulmoides is 8%.
6. The method for preparing the composition containing umbilical cord collagen active peptide according to claim 1, characterized in that: The mass fraction of the umbilical cord collagen active peptide is 18%.
7. The method for preparing the composition containing umbilical cord collagen active peptide according to claim 1, characterized in that: The mass fraction of the musk is 3%.
8. A composition containing umbilical cord collagen active peptides prepared by the method for preparing a composition containing umbilical cord collagen active peptides according to any one of claims 1 to 7.