Library construction system for gene detection

Abstract

The invention discloses a library construction system for gene detection, and relates to the technical field of detection, the library construction system comprises: an extraction bin equipped with extraction equipment; library building equipment is mounted in the library building bin; the transfer bin is arranged between the extraction bin and the library building bin, the conveying mechanism penetrates through the transfer bin, and the extraction bin and the library building bin are selectively communicated with the transfer bin so that the conveying mechanism can convey a to-be-detected sample in the extraction bin to the library building bin through the transfer bin. According to the high-throughput sequencing laboratory, by arranging the transfer device, the conveying mechanism conveys the to-be-tested sample in the extraction bin to the library building bin through the transfer bin, and the extraction bin and the library building bin can be arranged in the same laboratory area, so that the occupied area of the high-throughput sequencing laboratory is effectively reduced; according to the method, the construction cost of a high-throughput sequencing laboratory is saved, the landing time of gene detection products is saved, full-process automation from an extraction link to a library construction link is realized, detection result errors caused by manual operation errors are effectively prevented, and the accuracy of the detection results is improved.

Description

Technical Field

[0001] The present invention relates to the field of detection technology, and in particular to a library construction system for gene detection. Background Art

[0002] In the related art, in order to avoid cross-contamination of samples in high-throughput sequencing laboratories (NGS), extraction equipment and library construction equipment are respectively set up in corresponding laboratory areas. However, setting up the extraction equipment and library construction equipment in different laboratory areas will result in high construction costs, long construction time, and large required floor space for the high-throughput sequencing laboratory. In addition, the process connection between the extraction link and the library construction link requires professional personnel to operate, which cannot effectively prevent erroneous test results caused by human operational errors. Summary of the invention

[0003] The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, one purpose of the present invention is to provide a library construction system for gene detection, reduce the floor space of a high-throughput sequencing laboratory, save the construction cost and time of a high-throughput sequencing laboratory, and improve the accuracy of the detection results.

[0004] The library construction system for gene detection according to the present invention comprises: an extraction chamber, in which an extraction device is installed, and the extraction device is used to extract a sample to be tested; a library building chamber, in which a library building device is installed, and the library building device is used to convert the extracted sample to be tested into an identifiable standardized library, and the extraction chamber and the library building chamber are arranged along a first direction and are spaced apart; a transfer device, the transfer device comprises a transfer chamber and a conveying mechanism, the transfer chamber is arranged between the extraction chamber and the library building chamber, the conveying mechanism is penetrated in the transfer chamber along the first direction, and one end of the conveying mechanism extends into the extraction chamber, and the other end of the conveying mechanism extends into the library building chamber, and both the extraction chamber and the library building chamber can be selectively connected to the transfer chamber, so that the conveying mechanism transports the sample to be tested in the extraction chamber to the library building chamber through the transfer chamber.

[0005] According to the library construction system for gene detection of the present invention, by setting a transfer device, the conveying mechanism transports the sample to be tested in the extraction chamber to the library construction chamber through the transfer chamber, and the extraction chamber and the library construction chamber can be set in the same laboratory area, which effectively reduces the floor space of the high-throughput sequencing laboratory. The library construction system can completely simulate the environment required for sample extraction and library construction, saving the construction cost of the high-throughput sequencing laboratory and the time for the implementation of gene detection products. In addition, the whole process from the extraction link to the library construction link is automated, which effectively prevents the error of the detection result caused by human operation error and improves the accuracy of the detection result.

[0006] In some examples of the present invention, a first notch is formed on a first extraction chamber side wall opposite to the transfer chamber, and a second notch is formed on a first side wall of the transfer chamber opposite to the first extraction chamber side wall, the first notch and the second notch are opposite and connected along a first direction, the second notch is selectively opened or closed to connect or separate the extraction chamber and the transfer chamber, and when the second notch is opened, the conveying mechanism transports the sample to be tested to the transfer chamber through the first notch and the second notch.

[0007] In some examples of the present invention, the first side wall is movably provided with a first sealing door, and the first sealing door is used to open or close the second gap.

[0008] In some examples of the present invention, the first sealing door is slidably disposed on the first side wall along the second direction to open or close the second gap, and the first direction is perpendicular to the second direction.

[0009] In some examples of the present invention, a first through hole is also formed on the side wall of the first extraction bin, the first through hole and the first notch are adjacent and connected along the second direction, a second through hole is formed on the first side wall, the second through hole and the second notch are adjacent and connected along the second direction, the first through hole and the second through hole are opposite and connected along the first direction, and the conveying mechanism is passed through the first through hole and the second through hole, and the first direction and the second direction are perpendicular.

[0010] In some examples of the present invention, the first sealing door has an abutting end, and a first sealing member is fixed to the abutting end. When the first sealing door closes the second gap, the first sealing member abuts and seals against the conveying mechanism.

[0011] In some examples of the present invention, along the third direction, two conveying mechanism side walls of the conveying mechanism are both abutted and sealed against the transfer bin and the extraction bin, and the first direction, the second direction and the third direction are perpendicular to each other.

[0012] In some examples of the present invention, a second seal is fixedly provided between the first side wall and the first extraction bin side wall, the second seal abuts against both the first side wall and the first extraction bin side wall, the second seal is arranged around the second notch along the circumference of the second notch, and the second seal is arranged around the first notch along the circumference of the first notch.

[0013] In some examples of the present invention, a third seal is fixedly provided between the first side wall and the first extraction bin side wall, the third seal abuts against both the first side wall and the first extraction bin side wall, the third seal is located on the side of the second seal away from the first notch, and the third seal is arranged around the second seal along the circumference of the second seal.

[0014] In some examples of the present invention, the third sealing member has a first sub-sealing strip, a second sub-sealing strip and a third sub-sealing strip, the second sub-sealing strip is connected between the first sub-sealing strip and the third sub-sealing strip, the first sub-sealing strip and the third sub-sealing strip both extend along the second direction and are respectively arranged on two side edges of the first side wall opposite to each other along the third direction, along the second direction, the second sub-sealing strip is arranged on the upper edge of the first side wall, and the first direction, the second direction and the third direction are perpendicular to each other.

[0015] In some examples of the present invention, the third sealing member also has a fourth sub-sealing strip and a fifth sub-sealing strip. Along the third direction, the fourth sub-sealing strip and the fifth sub-sealing strip are respectively located on both sides of the conveying mechanism. Along the second direction, the lower end of the first sub-sealing strip is connected to the fourth sub-sealing strip, and the lower end of the third sub-sealing strip is connected to the fifth sub-sealing strip. The fourth sub-sealing strip and the fifth sub-sealing strip both extend along the third direction and are arranged at the lower edge of the first side wall. The fourth sub-sealing strip and the fifth sub-sealing strip are respectively abutted and sealed against the two conveying mechanism side walls of the conveying mechanism.

[0016] In some examples of the present invention, the warehouse building warehouse has a first warehouse building warehouse side wall opposite to the transfer warehouse, the transfer warehouse has a second side wall opposite to the first warehouse building warehouse side wall, the first warehouse building warehouse side wall and the first extraction warehouse side wall are constructed with the same structure, the second side wall and the first side wall are constructed with the same structure, and the assembly method of the first warehouse building warehouse side wall and the second side wall is the same as the assembly method of the first extraction warehouse side wall and the first side wall.

[0017] In some examples of the present invention, at least one side wall of the extraction bin is provided with a first bin door, which is used to open or close the extraction bin, and / or at least one side wall of the warehouse building bin is provided with a second bin door, which is used to open or close the warehouse building bin.

[0018] In some examples of the invention, the first bin door and the second bin door are identical.

[0019] In some examples of the present invention, the first bin door and the second bin door both include: a door frame and a movable door, the door frame is formed with a taking-in and putting hole, and the movable door can be movably arranged on the door frame to open or close the taking-in and putting hole.

[0020] In some examples of the present invention, the movable door and the door frame are arranged opposite to each other and are slidably arranged on the door frame along a second direction, the second direction is perpendicular to the first direction, a fourth seal is fixedly provided on the surface of the door frame facing the movable door, the fourth seal abuts against the movable door, a fifth seal is fixedly provided on the surface of the movable door facing the door frame, the fifth seal abuts against the door frame, and when the movable door closes the access hole, the fourth seal and the fifth seal are arranged around the access hole.

[0021] In some examples of the present invention, the fourth seal includes: a sixth sub-sealing strip and two seventh sub-sealing strips, along the second direction, the sixth sub-sealing strip is located at the upper edge of the door frame, the two seventh sub-sealing strips extend along the second direction and are respectively located at two side edges of the door frame, and the sixth sub-sealing strip is connected between the two fifth sub-sealing strips; the fifth seal includes: an eighth sub-sealing strip and two ninth sub-sealing strips, along the second direction, the eighth sub-sealing strip is located at the lower edge of the movable door, the two ninth sub-sealing strips extend along the second direction and are respectively located at two side edges of the movable door, the eighth sub-sealing strip is connected between the two ninth sub-sealing strips, and the seventh sub-sealing strip and the corresponding ninth sub-sealing strip are arranged along the width direction of the access hole.

[0022] In some examples of the present invention, the air pressure in the extraction chamber is greater than the air pressure in the storage chamber.

[0023] In some examples of the present invention, the air pressure in the transfer chamber is lower than the air pressure in the extraction chamber and higher than the air pressure in the storage chamber.

[0024] In some examples of the present invention, the extraction chamber has a first gas drive assembly and a first filter element. The first gas drive assembly is used to drive gas into and out of the extraction chamber to adjust the air pressure in the extraction chamber, and the first filter element is used to filter the gas flowing into the extraction chamber.

[0025] In some examples of the present invention, the warehouse building warehouse has a second gas drive component and a second filter element. The second gas drive component is used to drive gas into and out of the warehouse building warehouse to adjust the air pressure in the warehouse building warehouse, and the second filter element is used to filter the gas flowing into the warehouse building warehouse.

[0026] In some examples of the present invention, the conveying mechanism includes a driving mechanism, a guide rail and a placement table. The guide rail passes through the transfer bin along a first direction, and the two ends of the guide rail extend into the extraction bin and the library building bin respectively. The placement table is located outside the guide rail and is used to place the samples to be tested. At least a portion of the driving mechanism is disposed in the guide rail and is transmission-connected to the placement table. The driving mechanism is used to drive the placement table to reciprocate relative to the guide rail along the first direction so that the placement table moves into one of the extraction bin, the library building bin and the transfer bin.

[0027] In some examples of the present invention, the guide rail has a guide rail wall facing the placement table, the guide rail wall is formed with an avoidance hole extending along a first direction, the driving mechanism includes a driving rod, the driving rod is passed through the avoidance hole, the avoidance hole is provided with a sixth sealing member, the sixth sealing member is used to seal the avoidance hole, the sixth sealing member includes a first sealing strip and a second sealing strip, the first sealing strip and the second sealing strip both extend along the first direction and are respectively located on both sides of the driving rod, and the first sealing strip and the second sealing strip both abut against the driving rod for sealing.

[0028] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which: Figure 1 is a schematic diagram of the structure of a library construction system according to an embodiment of the present invention; Figure 2 is a schematic structural diagram of a transfer device according to an embodiment of the present invention; Figure 3 yes Figure 2 A magnified image of point A; Figure 4 is an exploded view of a first compartment door according to an embodiment of the present invention; Figure 5 is a structural schematic diagram of a first door (hidden movable door) according to an embodiment of the present invention; Figure 6 is a schematic structural diagram of a conveying mechanism according to an embodiment of the present invention; Figure 7 It is a schematic structural diagram from another angle of the transmission device according to an embodiment of the present invention.

[0030] Reference numerals: Library construction system 100; Extraction chamber 1; first extraction chamber side wall 11; first notch 111; first through hole 112; second sealing member 113; The third sealing member 114; the first sub-sealing strip 1141; the second sub-sealing strip 1142; the third sub-sealing strip 1143; the fourth sub-sealing strip 1144; The first door 12; the second door 13; the door frame 121; the fourth sealing member 1211; the sixth sub-sealing strip 1212; the seventh sub-sealing strip 1213; Movable door 122; fifth sealing member 1221; eighth sub-sealing strip 1222; ninth sub-sealing strip 1223; access hole 123; first gas drive assembly 13; first sub-gas drive member 131; first filter member 14; Warehouse building 2; second gas driving component 21; second sub-gas driving component 211; second filter component 22; Transfer device 3; transfer chamber 31; first side wall 311; second notch 312; first sealing door 313; abutting end 315; first sealing member 316; second side wall 317; Conveying mechanism 32; conveying mechanism side wall 321; driving mechanism 322; driving rod 3221; driving motor 3222; transmission belt 3223; connecting member 3224; guide rail 323; guide rail wall 3231; pressure strip 3232; sixth sealing member 3233; first sealing strip 3234; second sealing strip 3235; fixed base 3236; placement table 324. DETAILED DESCRIPTION

[0031] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0032] Reference below Figure 1-Figure 7 A library construction system 100 according to an embodiment of the present invention is described.

[0033] like Figure 1-Figure 2 As shown, the library construction system 100 according to the present invention includes: an extraction chamber 1, in which an extraction device is installed, and the extraction device is used to extract a sample to be tested; a library building chamber 2, in which a library building device is installed, and the library building device is used to convert the extracted sample to be tested into an identifiable standardized library, and the extraction chamber 1 and the library building chamber 2 are arranged along a first direction and are spaced apart; a transfer device 3, the transfer device 3 includes a transfer chamber 31 and a conveying mechanism 32, the transfer chamber 31 is arranged between the extraction chamber 1 and the library building chamber 2, the conveying mechanism 32 is penetrated in the transfer chamber 31 along the first direction, and one end of the conveying mechanism 32 extends into the extraction chamber 1, and the other end of the conveying mechanism 32 extends into the library building chamber 2, and the extraction chamber 1 and the library building chamber 2 can both be selectively connected to the transfer chamber 31, so that the conveying mechanism 32 transports the sample to be tested in the extraction chamber 1 to the library building chamber 2 through the transfer chamber 31.

[0034] Among them, the extraction chamber 1 is equipped with an extraction device for extracting nucleic acids. The extraction device can be assembled to the extraction chamber 1 by means of bolts, card connections, etc., but is not limited to the extraction device. The extraction device is used to extract the sample to be tested. The library construction chamber 2 is equipped with a library construction device for constructing a sequencing library. The library construction device can be assembled to the library construction chamber 2 by means of bolts, card connections, etc., but is not limited to the extraction device. The library construction device is used to convert the extracted sample to be tested into an identifiable standardized library.

[0035] The first direction is Figure 2In the X direction, the extraction chamber 1 and the warehouse building chamber 2 are arranged along the first direction, and the extraction chamber 1 and the warehouse building chamber 2 are spaced apart along the first direction to reduce the risk of cross contamination between the extraction chamber 1 and the warehouse building chamber 2. Along the first direction, the transfer chamber 31 is arranged between the extraction chamber 1 and the warehouse building chamber 2, and the conveying mechanism 32 is penetrated through the transfer chamber 31 along the first direction. Such an arrangement can make the arrangement of the conveying mechanism 32 reasonable, so that one end of the conveying mechanism 32 extends into the extraction chamber 1, and the other end of the conveying mechanism 32 extends into the warehouse building chamber 2.

[0036] Sealed doors can be provided between the extraction chamber 1 and the transfer chamber 31, and between the library building chamber 2 and the transfer chamber 31. When the corresponding sealed door is opened, the corresponding extraction chamber 1 or the corresponding library building chamber 2 is connected to the transfer chamber 31. When the corresponding sealed door is closed, the corresponding extraction chamber 1 or the corresponding library building chamber 2 is not connected to the transfer chamber 31. This arrangement allows the extraction chamber 1 and the library building chamber 2 to be selectively connected to the transfer chamber 31, so that the conveying mechanism 32 can transport the samples to be tested in the extraction chamber 1 to the library building chamber 2 through the transfer chamber 31.

[0037] By setting up the transfer device 3, so that the conveying mechanism 32 can transport the samples to be tested in the extraction chamber 1 to the library construction chamber 2 through the transfer chamber 31, the extraction chamber 1 and the library construction chamber 2 can be set in the same laboratory area, so that the library construction system 100 occupies a smaller area. For example, in this way, the library construction system 100 can occupy an area of ​​about 6 square meters (including detection equipment and gene analysis all-in-one machine), and the extraction equipment and the library construction equipment are respectively set in different laboratory areas, occupying an area of ​​about 80 square meters. It can be seen that the library construction system 100 effectively reduces the floor space of the high-throughput sequencing laboratory, saves the construction cost and time of the high-throughput sequencing laboratory, and the conveying mechanism 32 transports the samples to be tested in the extraction chamber 1 to the library construction chamber 2 through the transfer chamber 31, which can realize the process automation between the extraction link and the library construction link, effectively prevent the detection result errors caused by manual operation errors, improve the standardization, normalization and convenience of genetic testing, and thus improve the accuracy of the detection results.

[0038] Specifically, the extraction device is assembled in the extraction chamber 1, the library building device is assembled in the library building chamber 2, the extraction chamber 1 and the library building chamber 2 are arranged along the first direction and the extraction chamber 1 and the library building chamber 2 are spaced apart, and the transfer chamber 31 is assembled in the interval between the extraction chamber 1 and the library building chamber 2, so that the extraction chamber 1 and the library building chamber 2 can be selectively connected to the transfer chamber 31. The conveying mechanism 32 is arranged in the transfer chamber 31, and one end of the conveying mechanism 32 extends into the extraction chamber 1, and the other end of the conveying mechanism 32 extends into the library building chamber 2.

[0039] The library construction system 100 is provided with a detection program. When the sample to be tested needs to be transferred from the extraction chamber 1 to the library construction chamber 2 through the transfer chamber 31, the air pressure in the extraction chamber 1 is greater than the air pressure in the library construction chamber 2. This application takes the air pressure in the library construction chamber 2 as 5Pa and the air pressure in the extraction chamber 1 as 10Pa as an example for explanation. The sample to be tested and the reagents and consumables required by the extraction equipment are placed in the extraction chamber 1, and the reagents and consumables required for library construction are placed in the library construction chamber 2. The extraction equipment in the extraction chamber 1 extracts the sample to be tested. After the extraction is completed, the detection program first controls the connection between the extraction chamber 1 and the transfer chamber 31. It should be noted that at this time, the library construction chamber 2 and the transfer chamber 31 are not connected, and the air pressure in the extraction chamber 1 and the transfer chamber 31 will be quickly exchanged and balanced, and finally the overall pressure will drop to slightly below 10Pa (at this time, the air pressure in the library construction chamber 2 is still about 5Pa).

[0040] At the same time, the detection program controls the conveying mechanism 32 to transport the sample to be tested after extraction in the extraction chamber 1 to the transfer chamber 31. After the sample to be tested completely enters the transfer chamber 31, the detection program controls the extraction chamber 1 and the transfer chamber 31 to be disconnected, and the warehouse building chamber 2 and the transfer chamber 31 to be connected. Based on the same principle, the air pressure in the transfer chamber 31 and the warehouse building chamber 2 will quickly exchange and reach equilibrium, and finally the overall pressure will rise to slightly above 5Pa (at this time the air pressure in the extraction chamber 1 returns to about 10Pa).

[0041] During this process, the air in the transfer chamber 31 and the library building chamber 2 will not flow to the extraction chamber 1. After the detection program controls the conveying mechanism 32 to continue to transport the samples to be tested in the transfer chamber 31 to the library building chamber 2, the detection program controls the library building chamber 2 and the transfer chamber 31 to be disconnected, and the library building equipment in the library building chamber 2 is used to convert the extracted samples to be tested into an identifiable standardized library. During the whole process, the airflow always flows from the extraction chamber 1 to the library building chamber 2 through the transfer chamber 31, and the flow direction is single, which effectively reduces the risk of cross-contamination of gases in the extraction chamber 1 and the library building chamber 2.

[0042] Therefore, by setting up the transfer device 3 so that the conveying mechanism 32 can transport the sample to be tested in the extraction chamber 1 to the library construction chamber 2 through the transfer chamber 31, the extraction chamber 1 and the library construction chamber 2 can be set in the same laboratory area, which effectively reduces the floor space of the high-throughput sequencing laboratory. The library construction system 100 can completely simulate the environment required for sample extraction and library construction, save the construction cost of the high-throughput sequencing laboratory and save the time for the implementation of genetic testing products. In addition, the process automation between the extraction link and the library construction link is realized, which effectively prevents the error in the test result caused by human operation error and improves the accuracy of the test result.

[0043] In some examples of the present invention, Figure 1 and Figure 2As shown, a first notch 111 is formed on the first extraction chamber side wall 11 opposite to the extraction chamber 1 and the transfer chamber 31, and a second notch 312 is formed on the first side wall 311 of the transfer chamber 31 opposite to the first extraction chamber side wall 11. The first notch 111 and the second notch 312 are opposite and connected along a first direction, and the second notch 312 is selectively opened or closed to connect or separate the extraction chamber 1 and the transfer chamber 31, and when the second notch 312 is opened, the conveying mechanism 32 transports the sample to be tested to the transfer chamber 31 through the first notch 111 and the second notch 312.

[0044] The extraction chamber 1 has a first extraction chamber side wall 11, which is a side wall opposite to the extraction chamber 1 and the transfer chamber 31, and a first notch 111 is formed on the first extraction chamber side wall 11. The transfer chamber 31 has a first side wall 311, which is a side wall opposite to the first extraction chamber side wall 11 of the transfer chamber 31, and a second notch 312 is formed on the first side wall 311. The first notch 111 and the second notch 312 are opposite to each other along a first direction, and the first notch 111 and the second notch 312 are connected to each other, so that when the first notch 111 and the second notch 312 are connected, the conveying mechanism 32 can be smoothly avoided, so that the conveying mechanism 32 can transport the sample to be tested in the extraction chamber 1 to the transfer chamber 31.

[0045] The second gap 312 is selectively opened or closed to connect or separate the extraction chamber 1 and the transfer chamber 31. For example, the second gap 312 can be provided with a sealing door. When the sealing door is opened, the second gap 312 is opened, and the conveying mechanism 32 can smoothly transport the sample to be tested to the transfer chamber 31 through the first gap 111 and the second gap 312. When the sealing door is closed, the second gap 312 is closed, thereby reducing the risk of gas exchange between the extraction chamber 1 and the transfer chamber 31, thereby reducing the risk of cross-contamination of the sample to be tested.

[0046] In some examples of the present invention, Figure 2 As shown, the first side wall 311 is movably provided with a first sealing door 313 , and the first sealing door 313 is used to open or close the second notch 312 .

[0047] Among them, the first side wall 311 is movably provided with a first sealing door 313, and the first sealing door 313 is the sealing door of the above-mentioned embodiment. As some embodiments of the present application, the first side wall 311 is slidably provided with a first sealing door 313. As some embodiments of the present application, the first side wall 311 is rotatably provided with a first sealing door 313. The first sealing door 313 is used to open or close the second notch 312. When the first sealing door 313 is opened, the second notch 312 is opened, and the conveying mechanism 32 can smoothly transport the sample to be tested to the transfer chamber 31 through the first notch 111 and the second notch 312. When the first sealing door 313 is closed, the second notch 312 is closed, reducing the risk of gas exchange between the extraction chamber 1 and the transfer chamber 31, thereby reducing the risk of cross contamination of the sample to be tested. By movably providing the first sealing door 313 on the first side wall 311, the difficulty of controlling the opening and closing of the second notch 312 can be reduced, thereby facilitating the transportation of the sample to be tested, and achieving the effect that the conveying mechanism 32 can selectively transport the sample to be tested to the transfer chamber 31 through the first notch 111 and the second notch 312.

[0048] In some examples of the present invention, Figure 2 As shown, the first sealing door 313 is slidably disposed on the first side wall 311 along the second direction to open or close the second notch 312 , and the first direction is perpendicular to the second direction.

[0049] Among them, along the second direction, that is, Figure 2 In the Y direction, that is, the height direction of the library construction system 100 or the up-down direction of the library construction system 100, the first sealing door 313 is slidably disposed on the first side wall 311 to open or close the second notch 312, and the first direction is perpendicular to the second direction, that is, the X direction is perpendicular to the Y direction.

[0050] Specifically, when the first sealing door 313 slides upward along the second direction, the first sealing door 313 is opened, the second notch 312 is opened, and the conveying mechanism 32 can smoothly convey the sample to be tested to the transfer chamber 31 through the first notch 111 and the second notch 312. When the first sealing door 313 slides downward along the second direction, the first sealing door 313 is closed, and the second notch 312 is closed, thereby reducing the risk of gas exchange between the extraction chamber 1 and the transfer chamber 31, thereby reducing the risk of cross contamination of the sample to be tested.

[0051] By providing a first sealing door 313 that can slide along the second direction, during the opening or closing process of the first sealing door 313, the risk of the first sealing door 313 fanning the gas in the transfer chamber 31 into the extraction chamber 1 can be reduced, thereby reducing the risk of contamination of the sample to be tested in the extraction chamber 1, and further reducing the risk of cross-contamination of the sample to be tested.

[0052] In some examples of the present invention, Figure 1 and Figure 2 As shown, the first extraction bin side wall 11 is also formed with a first through hole 112, the first through hole 112 and the first notch 111 are adjacent and connected along the second direction, the first side wall 311 is formed with a second through hole, the second through hole and the second notch 312 are adjacent and connected along the second direction, the first through hole 112 and the second through hole are opposite and connected along the first direction, the conveying mechanism 32 is penetrated through the first through hole 112 and the second through hole, and the first direction and the second direction are perpendicular.

[0053] The first extraction chamber side wall 11 is further formed with a first through hole 112, and along the second direction, the first through hole 112 and the first notch 111 are adjacently arranged, and the first through hole 112 and the first notch 111 are connected. The first side wall 311 is formed with a second through hole, and along the second direction, the second through hole and the second notch 312 are adjacently arranged, and the second through hole and the second notch 312 are connected. The first direction is perpendicular to the second direction, that is, the X direction is perpendicular to the Y direction.

[0054] Along the first direction, the first through hole 112 and the second through hole are arranged opposite to each other, and the first through hole 112 and the second through hole are connected. The first through hole 112 and the second through hole are both used to avoid the conveying mechanism 32, so that the conveying mechanism 32 can be passed through the first through hole 112 and the second through hole, so that one end of the conveying mechanism 32 along the first direction can be passed through the first side wall 311 of the transfer chamber 31, and one end of the conveying mechanism 32 extends into the extraction chamber 1, thereby achieving the effect that the conveying mechanism 32 can transport the sample to be tested in the extraction chamber 1 to the transfer chamber 31.

[0055] In some examples of the present invention, Figure 2 and Figure 3 As shown, the first sealing door 313 has an abutting end 315 , and a first sealing member 316 is fixedly disposed on the abutting end 315 . When the first sealing door 313 closes the second notch 312 , the first sealing member 316 abuts and seals against the conveying mechanism 32 .

[0056] Among them, the first sealing door 313 has an abutting end 315. Along the second direction, the lower end of the first sealing door 313 is the abutting end 315. The abutting end 315 is fixed with a first sealing member 316. For example, the first sealing member 316 can be bonded to the abutting end 315, or the first sealing member 316 can be clamped on the abutting end 315. The first sealing member 316 can be but not limited to being constructed of rubber, silicone, foam and other materials. When the first sealing door 313 closes the second notch 312, the first sealing member 316 abuts and seals with the conveying mechanism 32 to smoothly seal the gap between the conveying mechanism 32 and the first sealing door 313, thereby reducing the risk of gas exchange between the extraction chamber 1 and the transfer chamber 31 when the first sealing door 313 closes the second notch 312, thereby reducing the risk of contamination of the sample to be tested in the extraction chamber 1.

[0057] In some examples of the present invention, Figure 2 As shown, along the third direction, the two conveying mechanism side walls 321 of the conveying mechanism 32 are both sealed against the transfer bin 31 and the extraction bin 1 , and the first direction, the second direction and the third direction are perpendicular to each other.

[0058] Among them, along the third direction, that is, Figure 2 In the Z direction, the conveying mechanism 32 has two conveying mechanism side walls 321, and the two conveying mechanism side walls 321 are both abutted and sealed with the transfer chamber 31 and the extraction chamber 1. For example, foam, silicone, rubber, etc. can be set in the gap between the two conveying mechanism side walls 321 and the transfer chamber 31 and the extraction chamber 1 to achieve the effect of both the two conveying mechanism side walls 321 abutting and sealing with the transfer chamber 31 and the extraction chamber 1, thereby reducing the risk of external air entering the transfer chamber 31 or the extraction chamber 1 through the gap between the conveying mechanism 32 and the transfer chamber 31 or the gap between the conveying mechanism 32 and the extraction chamber 1 during the process of conveying the sample by the conveying mechanism 32, thereby reducing the risk of contaminating the sample to be tested during transportation, which is beneficial to improving the accuracy of the test results.

[0059] In some examples of the present invention, Figure 2 As shown, a second seal 113 is fixedly provided between the first side wall 311 and the first extraction chamber side wall 11, the second seal 113 is in contact with both the first side wall 311 and the first extraction chamber side wall 11, the second seal 113 is arranged around the second notch 312 along the circumference of the second notch 312, and the second seal 113 is arranged around the first notch 111 along the circumference of the first notch 111.

[0060] Among them, a second seal 113 is fixedly provided between the first side wall 311 and the first extraction bin side wall 11. The second seal 113 can be but not limited to being constructed of materials such as rubber, silicone, foam, etc. The second seal 113 abuts against the first side wall 311 and the first extraction bin side wall 11. As some embodiments of the present application, a double-sided adhesive strip may be provided at one end of the second seal 113 along the first direction. The end of the second seal 113 provided with the double-sided adhesive strip is bonded to the first side wall 311, and the end of the second seal 113 not provided with the double-sided adhesive strip abuts against the first extraction bin side wall 11, so that the second seal 113 seals the gap between the first side wall 311 and the first extraction bin side wall 11.

[0061] As some embodiments of the present application, both ends of the second sealing member 113 along the first direction may be provided with double-sided adhesive strips, and the two ends of the second sealing member 113 along the first direction are respectively bonded to the first side wall 311 and the first extraction chamber side wall 11, so that the second sealing member 113 seals the gap between the first side wall 311 and the first extraction chamber side wall 11. The second sealing member 113 is arranged around the second notch 312 along the circumference of the second notch 312, and the second sealing member 113 is arranged around the first notch 111 along the circumference of the first notch 111. Such an arrangement can make the sealing between the extraction chamber 1 and the transfer chamber 31 reliable. During the opening process of the first sealing door 313, the risk of external air entering the extraction chamber 1 or the transfer chamber 31 through the gap between the extraction chamber 1 and the transfer chamber 31 can be reduced, further reducing the risk of the test sample contaminating the test sample during transportation, and further improving the accuracy of the test result.

[0062] In some examples of the present invention, Figure 2 As shown, a third seal 114 is fixedly provided between the first side wall 311 and the first extraction chamber side wall 11, the third seal 114 is in contact with both the first side wall 311 and the first extraction chamber side wall 11, the third seal 114 is located on the side of the second seal 113 away from the first notch 111, and the third seal 114 is arranged around the second seal 113 along the circumference of the second seal 113.

[0063] Among them, a third seal 114 is fixedly provided between the first side wall 311 and the first extraction bin side wall 11. The third seal 114 may be but is not limited to being constructed of materials such as rubber, silicone, foam, etc. The third seal 114 abuts against the first side wall 311 and the first extraction bin side wall 11. As some embodiments of the present application, a double-sided adhesive strip may be provided at one end of the third seal 114 along the first direction. The end of the third seal 114 provided with the double-sided adhesive strip is bonded to the first side wall 311, and the end of the third seal 114 not provided with the double-sided adhesive strip abuts against the first extraction bin side wall 11, so that the third seal 114 seals the gap between the first side wall 311 and the first extraction bin side wall 11.

[0064] As some embodiments of the present application, double-sided adhesive strips may be provided at both ends of the third seal 114 along the first direction, and the two ends of the third seal 114 along the first direction are respectively bonded to the first side wall 311 and the first extraction bin side wall 11, so that the third seal 114 seals the gap between the first side wall 311 and the first extraction bin side wall 11.

[0065] The third seal 114 is located on the side of the second seal 113 away from the first notch 111, and the third seal 114 is arranged around the second seal 113 along the circumference of the second seal 113. This arrangement can make the seal between the extraction chamber 1 and the transfer chamber 31 more reliable. During the opening process of the first sealing door 313, the second seal 113 and the third seal 114 are jointly sealed between the first side wall 311 and the first extraction chamber side wall 11, which greatly prevents ambient air from entering the interior of the library construction system 100 through the gap between the first side wall 311 and the first extraction chamber side wall 11, further reducing the risk of contaminating the test samples during transportation, and further improving the accuracy of the test results.

[0066] In some examples of the present invention, Figure 2 As shown, the third sealing member 114 has a first sub-sealing strip 1141, a second sub-sealing strip 1142 and a third sub-sealing strip 1143, the second sub-sealing strip 1142 is connected between the first sub-sealing strip 1141 and the third sub-sealing strip 1143, the first sub-sealing strip 1141 and the third sub-sealing strip 1143 both extend along the second direction and are respectively arranged on two side edges of the first side wall 311 opposite to each other along the third direction, along the second direction, the second sub-sealing strip 1142 is arranged on the upper edge of the first side wall 311, and the first direction, the second direction and the third direction are perpendicular to each other.

[0067] Among them, the second sub-sealing strip 1142 is connected between the first sub-sealing strip 1141 and the third sub-sealing strip 1143. Furthermore, the first sub-sealing strip 1141 and the third sub-sealing strip 1143 both extend along the second direction, and the first sub-sealing strip 1141 and the third sub-sealing strip 1143 are respectively arranged at two side edges of the first side wall 311 that are opposite to each other along the third direction. The second sub-sealing strip 1142 extends along the third direction, and the second sub-sealing strip 1142 is arranged at the upper edge of the first side wall 311. Such an arrangement can make the arrangement form of the third sealing member 114 reasonable, which is conducive to the third sealing member 114 reliably sealing the gap between the first side wall 311 and the first extraction chamber side wall 11, and improving the sealing effect of the third sealing member 114. The first direction, the second direction and the third direction are perpendicular to each other, that is, the X direction, the Y direction and the Z direction are perpendicular to each other.

[0068] In some examples of the present invention, Figure 2 and Figure 3As shown, the third sealing member 114 also has a fourth sub-sealing strip 1144 and a fifth sub-sealing strip. Along the third direction, the fourth sub-sealing strip 1144 and the fifth sub-sealing strip are respectively located on both sides of the conveying mechanism 32. Along the second direction, the lower end of the first sub-sealing strip 1141 is connected to the fourth sub-sealing strip 1144, and the lower end of the third sub-sealing strip 1143 is connected to the fifth sub-sealing strip. Both the fourth sub-sealing strip 1144 and the fifth sub-sealing strip extend along the third direction and are arranged at the lower edge of the first side wall 311. The fourth sub-sealing strip 1144 and the fifth sub-sealing strip are respectively abutted and sealed against the two conveying mechanism side walls 321 of the conveying mechanism 32.

[0069] Among them, along the third direction, that is, Figure 2 In the Z direction, the first sub-sealing strip 1141 and the third sub-sealing strip 1143 are respectively located on both sides of the conveying mechanism 32, and along the second direction, the lower end of the first sub-sealing strip 1141 is connected to the fourth sub-sealing strip 1144, and the lower end of the third sub-sealing strip 1143 is connected to the fifth sub-sealing strip, so that the fourth sub-sealing strip 1144 and the fifth sub-sealing strip are respectively located on both sides of the conveying mechanism 32, and the fourth sub-sealing strip 1144 and the fifth sub-sealing strip both extend along the third direction, and the fourth sub-sealing strip 1144 and the fifth sub-sealing strip are both arranged on the lower edge of the first side wall 311, and the fourth sub-sealing strip 1144 and the fifth sub-sealing strip are respectively abutted and sealed with the two conveying mechanism side walls 321 of the conveying mechanism 32, such an arrangement can make the arrangement form of the third seal 114 more reasonable, which is beneficial for the third seal 114 to seal the gap between the first side wall 311 and the first extraction bin side wall 11 at the outer edge of the first side wall 311, and is beneficial for improving the sealing effect of the third seal 114.

[0070] In some examples of the present invention, Figure 1 As shown, the warehouse building warehouse 2 has a first warehouse building warehouse side wall opposite to the transfer warehouse 31, the transfer warehouse 31 has a second side wall 317 opposite to the first warehouse building warehouse side wall, the first warehouse building warehouse side wall and the first extraction warehouse side wall 11 are constructed as the same structure, the second side wall 317 and the first side wall 311 are constructed as the same structure, and the assembly method of the first warehouse building warehouse side wall and the second side wall 317 is the same as the assembly method of the first extraction warehouse side wall 11 and the first side wall 311.

[0071] Among them, the library building warehouse 2 has a first library building warehouse side wall, and the first library building warehouse side wall is a side wall of the library building warehouse 2 opposite to the transfer warehouse 31. The transfer warehouse 31 has a second side wall 317, and the second side wall 317 is a side wall of the transfer warehouse 31 opposite to the first library building warehouse side wall. The first library building warehouse side wall and the first extraction warehouse side wall 11 are constructed as the same structure, the second side wall 317 and the first side wall 311 are constructed as the same structure, and the assembly method of the first library building warehouse side wall and the second side wall 317 is the same as the assembly method of the first extraction warehouse side wall 11 and the first side wall 311. Such a setting can make the structural setting of the library construction system 100 reasonable, which is conducive to reducing the production difficulty and assembly difficulty of the library construction system 100, and can also reduce the structural complexity of the library construction system 100, thereby reducing the production cost of the library construction system 100.

[0072] In some examples of the present invention, Figure 4 and Figure 5 As shown, at least one side wall of the extraction chamber 1 is provided with a first chamber door 12, which is used to open or close the extraction chamber 1, and / or at least one side wall of the storage chamber 2 is provided with a second chamber door 13, which is used to open or close the storage chamber 2.

[0073] Among them, as some embodiments of the present application, at least one side wall of the extraction bin 1 is provided with a first bin door 12, and the first bin door 12 is used to open or close the extraction bin 1. As some embodiments of the present application, at least one side wall of the library building bin 2 is provided with a second bin door 13, and the second bin door 13 is used to open or close the library building bin 2. As some embodiments of the present application, at least one side wall of the extraction bin 1 is provided with a first bin door 12, and the first bin door 12 is used to open or close the extraction bin 1, and at least one side wall of the library building bin 2 is provided with a second bin door 13, and the second bin door 13 is used to open or close the library building bin 2. This application is described by taking at least one side wall of the extraction bin 1 being provided with a first bin door 12, and the first bin door 12 is used to open or close the extraction bin 1, and at least one side wall of the library building bin 2 being provided with a second bin door 13, and the second bin door 13 is used to open or close the library building bin 2 as an example.

[0074] At least one side wall of the extraction chamber 1 is provided with a first chamber door 12, for example: one side wall of the extraction chamber 1 is provided with a first chamber door 12, or both side walls of the extraction chamber 1 are provided with a first chamber door 12, and the first chamber door 12 is used to open or close the extraction chamber 1. When the first chamber door 12 opens the extraction chamber 1, it is convenient for the staff to put the sample to be tested and the reagents and consumables required by the extraction equipment into the extraction chamber 1, so that the extraction equipment in the extraction chamber 1 can smoothly complete the extraction of the sample to be tested. When the first chamber door 12 closes the extraction chamber 1, the extraction equipment in the extraction chamber can smoothly complete the extraction of the sample to be tested, reducing the risk of the external environment contaminating the sample to be tested during the extraction process.

[0075] At least one side wall of the library building warehouse 2 is provided with a second door 13, for example: one side wall of the library building warehouse 2 is provided with a second door 13, or both side walls of the library building warehouse 2 are provided with a second door 13, and the second door 13 is used to open or close the library building warehouse 2. When the second door 13 opens the library building warehouse 2, it is convenient for the staff to put the reagents and consumables required by the library building equipment into the library building warehouse 2, so that the library building equipment in the library building warehouse 2 can successfully complete the library building work, or take the samples to be tested out of the library building warehouse 2. When the second door 13 closes the library building warehouse 2, the library building equipment in the library building warehouse 2 successfully completes the library building work, reducing the risk of the external environment contaminating the samples to be tested during the library building process.

[0076] In some examples of the present invention, Figure 4 and Figure 5 As shown, the first compartment door 12 and the second compartment door 13 are identical.

[0077] Among them, the first bin door 12 and the second bin door 13 are the same. Such an arrangement can make the arrangement of the first bin door 12 and the second bin door 13 reasonable. The first bin door 12 and the second bin door 13 of the same structure are conducive to reducing the production difficulty and assembly difficulty of the library construction system 100, and can also reduce the structural complexity of the library construction system 100. Furthermore, the first bin door 12 and the second bin door 13 are constructed as the same structure, which can reduce the number of molds for producing the first bin door 12 and the second bin door 13 during the production process, thereby reducing the production cost of the library construction system 100.

[0078] In some examples of the present invention, Figure 4 and Figure 5 As shown, the first compartment door 12 and the second compartment door 13 may include: a door frame 121 and a movable door 122 . The door frame 121 is formed with a take-in / take-out hole 123 . The movable door 122 may be movably disposed on the door frame 121 to open or close the take-in / take-out hole 123 .

[0079] Among them, the door frame 121 is formed with a loading and unloading hole 123, and the movable door 122 can be movably arranged on the door frame 121, for example: the movable door 122 can be slidably arranged on the door frame 121, or the movable door 122 can be rotatably arranged on the door frame 121, so that the movable door 122 can open or close the loading and unloading hole 123. When the movable door 122 opens the loading and unloading hole 123, the staff can put the corresponding reagent consumables into the corresponding extraction chamber 1 or the corresponding library construction chamber 2 through the loading and unloading hole 123, and the staff can put the sample to be tested into the extraction chamber 1 through the loading and unloading hole 123 or take the sample to be tested out of the library construction chamber 2 through the loading and unloading hole 123, so that the library construction system 100 can detect the sample to be tested multiple times.

[0080] In some examples of the present invention, Figure 4 and Figure 5As shown, the movable door 122 and the door frame 121 are arranged opposite to each other and are slidably arranged on the door frame 121 along a second direction, the second direction is perpendicular to the first direction, a fourth sealing member 1211 is fixedly provided on the surface of the door frame 121 facing the movable door 122, the fourth sealing member 1211 abuts against the movable door 122, a fifth sealing member 1221 is fixedly provided on the surface of the movable door 122 facing the door frame 121, the fifth sealing member 1221 abuts against the door frame 121, when the movable door 122 closes the access hole 123, the fourth sealing member 1211 and the fifth sealing member 1221 are arranged around the access hole 123.

[0081] The movable door 122 and the door frame 121 are arranged relative to each other, and along the second direction, the movable door 122 is slidably arranged on the door frame 121. This arrangement can make the movable door 122 arranged reasonably, reduce the risk of a large amount of exchange between the air in the extraction chamber 1 and the external environment air, and between the air in the building library and the external environment air during the opening or closing of the movable door 122, thereby further reducing the risk of contamination of the sample to be tested. The second direction is perpendicular to the first direction, that is, the X direction is perpendicular to the Y direction.

[0082] The door frame 121 is fixedly provided with a fourth seal 1211, which is arranged on the surface of the door frame 121 facing the movable door 122, and the fourth seal 1211 is in contact with the movable door 122. The movable door 122 is fixedly provided with a fifth seal 1221, which is formed on the surface of the movable door 122 facing the door frame 121, and the fifth seal 1221 is in contact with the door frame 121. When the movable door 122 closes the access hole 123, the fourth seal 1211 and the fifth seal 1221 are arranged around the access hole 123, for example: the fourth seal 1211 can be configured as an "n" type seal, the fifth seal 1221 can be configured as a "u" type seal, or the fourth seal 1211 can be configured as a "u" type seal, and the fifth seal 1221 can be configured as an "n" type seal. The fourth seal 1211 and the fifth seal 1221 can be fixed to the corresponding door frame 121 and the movable door 122 by means of bolts, bonding, etc., but not limited to. When the movable door 122 closes the access hole 123, the fourth seal 1211 and the fifth seal 1221 are arranged relatively along the second direction, and the fourth seal 1211 and the fifth seal 1221 are abutted, so that the fourth seal 1211 and the fifth seal 1221 jointly form a relatively sealed closed loop, so as to achieve a relatively sealed effect of the internal and external environments of the extraction chamber 1 and the storage chamber 2.

[0083] In some examples of the present invention, Figure 4 and Figure 5As shown, the fourth sealing member 1211 may include: a sixth sub-sealing strip 1212 and two seventh sub-sealing strips 1213, along the second direction, the sixth sub-sealing strip 1212 is located at the upper edge of the door frame 121, the two seventh sub-sealing strips 1213 extend along the second direction and are respectively located at two side edges of the door frame 121, and the sixth sub-sealing strip 1212 is connected between the two fifth sub-sealing strips; The fifth sealing member 1221 may include: an eighth sub-sealing strip 1222 and two ninth sub-sealing strips 1223. Along the second direction, the eighth sub-sealing strip 1222 is located at the lower edge of the movable door 122. The two ninth sub-sealing strips 1223 extend along the second direction and are respectively located at the two side edges of the movable door 122. The eighth sub-sealing strip 1222 is connected between the two ninth sub-sealing strips 1223. The seventh sub-sealing strip 1213 and the corresponding ninth sub-sealing strips 1223 are arranged along the width direction of the access hole 123.

[0084] Among them, along the second direction, the sixth sub-sealing strip 1212 is located at the upper edge of the door frame 121, the two seventh sub-sealing strips 1213 extend along the second direction, and the two seventh sub-sealing strips 1213 are respectively located at the two side edges of the door frame 121, and the sixth sub-sealing strip 1212 is connected between the two fifth sub-sealing strips, so that the fourth sealing member 1211 forms a continuous sealing structure. Along the second direction, the eighth sub-sealing strip 1222 is located at the lower edge of the movable door 122, the two ninth sub-sealing strips 1223 extend along the second direction, and the two ninth sub-sealing strips 1223 are respectively located at the two side edges of the movable door 122, and the eighth sub-sealing strip 1222 is connected between the two ninth sub-sealing strips 1223, so that the fifth sealing member 1221 forms a continuous sealing structure.

[0085] The seventh sub-sealing strip 1213 and the corresponding ninth sub-sealing strip 1223 are arranged along the width direction of the loading and unloading hole 123, and the seventh sub-sealing strip 1213 and the corresponding ninth sub-sealing strip 1223 can be set in abutment with each other, or a certain gap can be left, and the gap is relatively small (less than or equal to 2 mm). For example, the gap between the seventh sub-sealing strip 1213 and the corresponding ninth sub-sealing strip 1223 is about 2 mm, so as to achieve the effect of smooth sliding of the movable door 122 while minimizing the air outlet of the extraction chamber 1 and the storage chamber 2 when the movable door 122 closes the loading and unloading hole 123.

[0086] The eighth sub-sealing strip 1222 is in close contact with the table of the extraction chamber 1 or the storage chamber 2, so as to achieve a relatively airtight environment inside and outside the extraction chamber 1 or the storage chamber 2. After the experiment, when the movable door 122 is opened, the fifth sealing member 1221 moves with the movable door 122, and the ninth sub-sealing strip 1223 is finally parallel to the seventh sub-sealing strip 1213, and the eighth sub-sealing strip 1222 is finally parallel to the sixth sub-sealing strip 1212, which not only improves the sliding efficiency of the movable door 122, but also avoids the accelerated aging of the rubber strip due to exposure to the air.

[0087] This design breaks through the limitations of traditional logical deduction. When solid rubber strips were used in the early days, there were problems such as large pushing resistance of the movable door 122 and inconvenient installation. Later, hollow rubber strips were used, which have low friction resistance and can be fixed by screws, and the installation effect is good. The layout of the fifth seal 1221 and the sixth seal 3233 has also been optimized from the initial double full circle design to a combination of "n" type and "u" type, which not only facilitates the pushing of the movable door 122 and reduces material costs, but also reduces the loss and aging of the fifth seal 1221 and the sixth seal 3233 during the door opening and closing process, prolongs the service life, reduces maintenance costs, and achieves efficient sealing and low-friction sliding. Combined with optimized airflow control, the system can maintain a stable pressure difference and unidirectional airflow, while reducing the loss and maintenance costs of the fifth seal 1221 and the sixth seal 3233, and meets the requirements of sealing and single airflow direction of the library construction system 100 in a simple and low-cost manner.

[0088] In some examples of the present invention, Figure 1 As shown, the air pressure in the extraction chamber 1 is greater than the air pressure in the storage chamber 2.

[0089] Among them, the air pressure in the extraction chamber 1 is greater than the air pressure in the library construction chamber 2, for example: the air pressure in the library construction chamber 2 is 5Pa, and the air pressure in the extraction chamber 1 is 10Pa, or the air pressure in the library construction chamber 2 is 6Pa, and the air pressure in the extraction chamber 1 is 12Pa. By making the air pressure in the extraction chamber 1 greater than the air pressure in the library construction chamber 2, during the movement of the sample to be tested, the airflow in the library construction system 100 can always be transmitted from the extraction chamber 1 to the library construction chamber 2, and the flow direction of the airflow remains single, effectively reducing the risk of cross-contamination of gases in the extraction chamber 1 and the library construction chamber 2.

[0090] In some examples of the present invention, Figure 1 As shown, the air pressure in the transfer chamber 31 is lower than the air pressure in the extraction chamber 1 and higher than the air pressure in the storage chamber 2 .

[0091] Among them, the air pressure in the transfer chamber 31 is lower than the air pressure in the extraction chamber 1 and higher than the air pressure in the library building chamber 2. This setting can make the air pressure settings in the extraction chamber 1, the transfer chamber 31 and the library building chamber 2 reasonable. During the movement of the sample to be tested, the airflow in the library construction system 100 can always be transmitted from the extraction chamber 1 to the transfer chamber 31, and then from the transfer chamber 31 to the library building chamber 2, which is conducive to maintaining a single flow direction of the airflow and effectively reducing the risk of cross-contamination of gases in the extraction chamber 1 and the library building chamber 2.

[0092] Specifically, the transfer chamber 31 has a second sealing door. The library construction system 100 is provided with a detection program. When the sample to be tested needs to be transferred from the extraction chamber 1 to the library construction chamber 2 through the transfer chamber 31, the air pressure in the library construction chamber 2 is 5 Pa, and the air pressure in the extraction chamber 1 is 10 Pa. The detection program first controls the motor of the first sealing door 313 to open the first sealing door 313.

[0093] Since the sealing effect of the rubber strips between the transfer bin 31 and the extraction bin 1 is good, the air pressure in the transfer bin 31 is the same as the external environmental pressure, while the air pressure in the extraction bin 1 is about 10Pa higher than that in the transfer bin 31. In addition, since the volume of the extraction bin 1 is much larger than that of the transfer bin 31 (the volume of the extraction bin 1 is about 17 times the volume of the transfer bin 31), after the first sealing door 313 is opened, the air pressures in the extraction bin 1 and the transfer bin 31 will quickly exchange and reach equilibrium, and eventually the overall pressure will drop to slightly below 10Pa (at this time the air pressure in the warehouse building bin 2 is still about 5Pa).

[0094] At the same time, the detection program controls the conveying mechanism 32 to convey the sample to be tested to the transfer chamber 31, and then closes the first sealing door 313. Since the contact surface between the first sealing door 313 and the conveying mechanism 32 is sealed by the first sealing member 316, after the first sealing door 313 is closed, the air in the transfer chamber 31 is almost no longer exchanged with the air in the extraction chamber 1. At this time, the extraction chamber 1, the transfer chamber 31 and the library building chamber 2 all form relatively sealed independent spaces.

[0095] Then, the detection program controls the second sealed door motor to open the second sealed door. Based on the same principle, the air pressure in the transfer chamber 31 and the library building chamber 2 will quickly exchange and reach equilibrium, and eventually rise to slightly above 5Pa (at this time, the air pressure in the extraction chamber 1 returns to about 10Pa). At the same time, the air in the transfer chamber 31 and the library building chamber 2 will not flow to the extraction chamber 1. After the software controls the conveying mechanism 32 to transfer the sample to be tested from the transfer chamber 31 to the library building chamber 2, the second sealed door is closed.

[0096] At this time, the air between the transfer chamber 31 and the library building chamber 2 is no longer exchanged, and the extraction chamber 1, the transfer chamber 31 and the library building chamber 2 form a relatively sealed physical space. The sample to be tested is successfully transferred from the extraction chamber 1 to the library building chamber 2. During the whole process, the airflow always flows from the extraction chamber 1 through the transfer chamber 31 to the library building chamber 2, and the flow direction is single, which effectively reduces the risk of cross-contamination of gas in the extraction chamber 1 and the library building chamber 2.

[0097] In some examples of the present invention, Figure 1 As shown, the extraction chamber 1 has a first gas drive component 13 and a first filter element 14. The first gas drive component 13 is used to drive gas to enter and flow out of the extraction chamber 1 to adjust the air pressure in the extraction chamber 1. The first filter element 14 is used to filter the gas flowing into the extraction chamber 1.

[0098] Among them, the first filter element 14 can be but is not limited to being constructed as filter cotton, and the first gas drive component 13 is used to drive the gas into and out of the extraction chamber 1 to adjust the air pressure in the extraction chamber 1, so that the air pressure in the extraction chamber 1 is always higher than the air pressure in the storage chamber 2, thereby achieving the effect of a single airflow direction and reducing the risk of cross-contamination of gases in the extraction chamber 1 and the storage chamber 2.

[0099] The first gas driving component 13 may include multiple first sub-gas driving components 131. For example, the first gas driving component 13 may include two, three or more first sub-gas driving components 131. The first sub-gas driving component 131 may be but is not limited to being constructed as a fan, an air pump, etc. This application takes two first sub-gas driving components 131 as an example for explanation. One first sub-gas driving component 131 is used to drive the gas into the extraction chamber 1, and the other first sub-gas driving component 131 is used to drive the gas out of the extraction chamber 1. By adjusting the driving speed of the two first sub-gas driving components 131, the air pressure in the extraction chamber 1 can be adjusted.

[0100] Furthermore, the first filter element 14 is disposed at the first sub-gas driving element 131 for driving the gas to enter the extraction chamber 1 , so that the gas can enter the extraction chamber 1 after being filtered by the first filter element 14 , thereby reducing the risk of contamination of the sample to be tested in the extraction chamber 1 .

[0101] In some examples of the present invention, Figure 1 As shown, the warehouse building warehouse 2 has a second gas driving component 21 and a second filter element 22. The second gas driving component 21 is used to drive the gas to enter and flow out of the warehouse building warehouse 2 to adjust the air pressure in the warehouse building warehouse 2. The second filter element 22 is used to filter the gas flowing into the warehouse building warehouse 2.

[0102] Among them, the second filter element 22 can be but is not limited to being constructed as filter cotton, and the second gas driving component 21 is used to drive the gas to enter and flow out of the warehouse building warehouse 2 to adjust the air pressure in the warehouse building warehouse 2, so that the air pressure in the warehouse building warehouse 2 is always lower than the air pressure in the extraction warehouse 1, thereby achieving the effect of a single airflow direction and reducing the risk of cross-contamination of gases in the extraction warehouse 1 and the warehouse building warehouse 2.

[0103] The second gas drive assembly 21 may include multiple second sub-gas drivers 211. For example, the second gas drive assembly 21 may include two, three or more second sub-gas drivers 211. The second sub-gas drivers 211 may be but are not limited to being constructed as fans, air pumps, etc. This application takes two second sub-gas drivers 211 as an example for illustration. One second sub-gas driver 211 is used to drive the gas into the warehouse building warehouse 2, and the other second sub-gas driver 211 is used to drive the gas out of the warehouse building warehouse 2. By adjusting the driving speeds of the two second sub-gas drivers 211, the gas pressure in the warehouse building warehouse 2 can be adjusted.

[0104] Furthermore, the second filter element 22 is arranged at the second sub-gas driving element 211 used to drive the gas into the library building chamber 2, so that the gas can enter the library building chamber 2 after being filtered by the second filter element 22, thereby reducing the risk of contamination of the samples to be tested in the library building chamber 2.

[0105] In some examples of the present invention, Figure 6 As shown, the conveying mechanism 32 includes a driving mechanism 322, a guide rail 323 and a placement table 324. The guide rail 323 is arranged in the transfer chamber 31 along a first direction, and the two ends of the guide rail 323 extend into the extraction chamber 1 and the library building chamber 2 respectively. The placement table 324 is located outside the guide rail 323 and is used to place the sample to be tested. At least a part of the driving mechanism 322 is arranged in the guide rail 323 and is connected to the placement table 324 in transmission. The driving mechanism 322 is used to drive the placement table 324 to reciprocate relative to the guide rail 323 along the first direction, so that the placement table 324 moves into one of the extraction chamber 1, the library building chamber 2 and the transfer chamber 31.

[0106] The guide rail 323 extends along the first direction and penetrates the transfer chamber 31, and the two ends of the guide rail 323 along the first direction extend into the extraction chamber 1 and the library building chamber 2 respectively, and the placement table 324 is located outside the guide rail 323, so that the staff can place the samples to be tested on the placement table 324. At least part of the driving mechanism 322 is arranged in the guide rail 323, for example: part of the driving mechanism 322 is arranged in the guide rail 323, or the entire structure of the driving mechanism 322 is arranged in the guide rail 323, and the driving mechanism 322 is connected to the placement table 324 in a transmission manner, so that the driving mechanism 322 can drive the placement table 324 to reciprocate relative to the guide rail 323 along the first direction, which is conducive to the placement table 324 being smoothly moved into one of the extraction chamber 1, the library building chamber 2 and the transfer chamber 31, and then extracting and building the samples to be tested in the placement table 324 in sequence.

[0107] In some examples of the present invention, Figure 6 and Figure 7 As shown, the guide rail 323 has a guide rail wall 3231 facing the placement table 324, and the guide rail wall 3231 is formed with an avoidance hole extending along the first direction. The driving mechanism 322 includes a driving rod 3221, and the driving rod 3221 is inserted into the avoidance hole. The avoidance hole is provided with a sixth sealing member 3233, and the sixth sealing member 3233 is used to seal the avoidance hole. The sixth sealing member 3233 includes a first sealing strip 3234 and a second sealing strip 3235. The first sealing strip 3234 and the second sealing strip 3235 both extend along the first direction and are respectively located on both sides of the driving rod 3221. The first sealing strip 3234 and the second sealing strip 3235 both abut against the driving rod 3221 for sealing.

[0108] The guide rail 323 has a guide rail wall 3231, which forms a side wall of the guide rail 323 facing the placement table 324. The guide rail wall 3231 is formed with an avoidance hole extending along the first direction, and the avoidance hole can avoid the driving mechanism 322. Further, the driving mechanism 322 includes a driving rod 3221, which is inserted into the avoidance hole so that the driving rod 3221 can be fixedly connected with the placement table 324, thereby achieving the effect that the driving mechanism 322 drives the placement table 324 to move through the driving rod 3221. The avoidance hole is provided with a sixth sealing member 3233, which can be but not limited to rubber, silicone, foam, etc. The sixth sealing member 3233 is used to seal the avoidance hole to reduce the risk of external air entering the extraction warehouse 1, the warehouse building warehouse 2 or the transfer warehouse 31 through the avoidance hole.

[0109] The sixth sealing member 3233 includes a first sealing strip 3234 and a second sealing strip 3235, both of which extend along the first direction. Along the third direction, the first sealing strip 3234 and the second sealing strip 3235 are respectively located on both sides of the driving rod 3221, and both of the first sealing strip 3234 and the second sealing strip 3235 are sealed against the driving rod 3221 to reduce the risk of external air entering the extraction bin 1, the storage bin 2 or the transfer bin 31 through the gap between the driving rod 3221 and the guide rail wall 3231 when the driving rod 3221 moves along the first direction.

[0110] like Figure 7 As shown, the guide rail 323 can also be fixed with two pressure strips 3232, and the two pressure strips 3232 are respectively fixed at the ends of the first sealing strip 3234 and the second sealing strip 3235 that are away from each other. The first sealing strip 3234 and the second sealing strip 3235 use elastic materials. For example, the first sealing strip 3234 and the second sealing strip 3235 can be but not limited to being constructed of rubber or silicone materials. By setting the pressure strips 3232, the first sealing strip 3234 and the second sealing strip 3235 can be interference fit between the corresponding pressure strips and the driving rod 3221, which not only improves the sealing effect of the first sealing strip 3234 and the second sealing strip 3235, thereby further improving the sealing effect of the library construction system 100, but also can ensure the smooth sliding of the driving rod 3221.

[0111] The first sealing strip 3234 and the second sealing strip 3235 do not affect the back and forth movement of the driving rod 3221, and can keep the height of the placing platform 324 relatively stable, avoiding the shaking of the guide rail 323 due to excessive friction and affecting the accuracy of the height of the placing platform 324. This design ensures the stability of the driving mechanism 322 during operation, and at the same time fills the gap in the transmission direction of the guide rail 323, avoiding the exchange of air between the extraction chamber 1, the warehouse building chamber 2 or the transfer chamber 31 and the outside. In addition, the sixth sealing member 3233 in the guide rail 323 is in close contact with the rubber strips at the bottom of the first sealing door 313 and the second sealing door of the transfer chamber 31, which can effectively prevent the air flow between the extraction chamber 1, the warehouse building chamber 2 or the transfer chamber 31.

[0112] The guide rail 323 located in the extraction chamber 1 and the library building chamber 2 also plays the role of supporting the placement table 324. The guide rail 323 may have a fixed base 3236. By adjusting the height of the fixed base 3236 at the bottom of the guide rail 323, the placement table 324 can remain horizontal when it is at both ends of the guide rail 323 along the first direction (i.e., in the extraction chamber 1 or the library building chamber 2), ensuring that the automatic pipette in the chamber can adaptively perform accurate pipetting actions. This design meets the demand for automatic transfer of the placement table 324 between the extraction chamber 1 and the library building chamber 2, and realizes the function of the placement table 324 as a reagent exchange carrier for the extraction chamber 1 and the library building chamber 2.

[0113] As some embodiments of the present application, the driving mechanism 322 also includes a driving motor 3222, a transmission belt 3223 and a connecting piece 3224. The driving motor 3222 can be but is not limited to being constructed as a stepping motor, a linear motor, etc. The driving motor 3222 has an output end, which is transmission-connected to the transmission belt 3223 so that the driving motor 3222 can drive the transmission belt 3223 to rotate. The connecting piece 3224 and the transmission belt 3223 can be but is not limited to being fixedly connected by bolts, clamping, etc., and the connecting piece 3224 is fixedly connected to the driving rod 3221. The driving motor 3222 drives the transmission belt 3223 to drive the connecting piece 3224 to move. The movement of the connecting piece 3224 can drive the driving rod 3221 to drive the placement table 324 to reciprocate relative to the guide rail 323 along the first direction, which is conducive to the placement table 324 being smoothly moved into one of the extraction chamber 1, the library building chamber 2 and the transfer chamber 31, and then the samples to be tested in the placement table 324 are extracted and library built in turn.

[0114] It should be noted that, in the process of testing the sample to be tested, the placement table 324 only needs to be transferred from the extraction chamber 1 to the library building chamber 2. After the test operation is completed, the placement table 324 will be controlled to return to the extraction chamber 1 through the test program.

[0115] This application innovatively integrates the extraction chamber 1 and the library building chamber 2 into the same IVD device (in vitro diagnostic device), and realizes efficient and pollution-free transmission of the placement table 324 through the coordinated optimization of software and hardware. The extraction chamber 1 and the library building chamber 2 have a single-direction independent sealing system for airflow; the core component of the pollution-free system for object exchange between the extraction chamber 1 and the library building chamber 2 is the conveying mechanism 32. This module not only has the function of transmitting the sample to be tested, but also can realize the coordinated control of the motor and transmission belt 3223 of the conveying placement table 324 and the independent opening and closing of the first sealing door 313 and the second sealing door through the overall control of the software when connecting the extraction chamber 1 and the library building chamber 2. In the process of transmitting the sample to be tested, the module can maintain the relative stability of the air pressure in each chamber and the stability of the pressure difference between the extraction chamber 1 and the library building chamber 2, thereby ensuring the unidirectional flow of the airflow and minimizing the risk of cross contamination.

[0116] As some embodiments of the present application, aerosols are easily generated during the PCR (polymerase chain reaction) process of gene detection library construction, causing cross contamination. This method uses mineral oil to seal the liquid surface during the PCR process to avoid the generation of aerosols. In addition, the extraction chamber 1 and the library construction chamber 2 are regularly monitored for environmental pollution to ensure that the pollution in the chamber is lower than the positive detection threshold. In the whole process of using the library construction system 100 to detect gene products, the product quality control system is used for monitoring to determine that there is no cross contamination in the test results of the same batch.

[0117] Aerosols are easily generated during the library construction PCR process, so oil sealing technology is used. The specific steps of applying the oil sealing technology in this system are as follows: Step 1: The library construction system 100 is started, and the pressure difference between the extraction chamber 1 and the library construction chamber 2 is maintained at about 5Pa, and the airflow direction is single. Step 2: When the pathogen gene is detected, the sample to be tested, the product quality control and the blank are detected at the same time to monitor the whole process. Step 3: After the sample is extracted in the extraction chamber 1, it is transferred to the library construction chamber 2 through the transfer chamber 31 whose structure can control the airflow direction between the chambers. Step 4: Mineral oil is sealed during library construction PCR to avoid aerosol cross contamination. Step 5: After sequencing based on the second-generation sequencing platform, the integrated machine supporting the library construction system 100 performs quality control on the original sequencing data, removes the data from the host and obtains the results to be compared. Step 6: The integrated machine supporting the library construction system 100 will obtain the results to be compared and compare them with the comparison database pre-established in the system to determine the detection of the main pathogenic organisms.

[0118] Two methods for regular contamination detection are as follows: Surface wiping sampling method: After the swab is sampled at multiple points on the inner surface of the library construction system 100, routine detection is performed (steps 1 to 6).

[0119] Air precipitation sampling method: After the sampling tubes filled with physiological saline are placed at multiple points inside the library construction system 100 for 24 hours, routine testing is performed (steps 1 to 6).

[0120] As some embodiments of the present application, the library construction system 100 controls the operation of the equipment through program settings, and can automatically complete the full process experiment and analysis from sample to report. The construction of conventional NGS laboratories (high-throughput sequencing laboratories) needs to pay attention to the wind direction to form convection to reduce cross contamination, and the full-process automated all-in-one equipment can also meet this demand. By separating the two experimental chambers of the extraction chamber 1 and the library construction chamber 2, the extraction chamber 1 and the library construction chamber 2 are sealed with rubber strips around them, and are respectively equipped with HEPA (high-efficiency air filtration) systems, filter cotton and small fans to ensure that the air pressure difference in the extraction chamber 1 and the library construction chamber 2 is relatively stable. When the placement table 324 is transported by the conveying mechanism 32, the opening order of the first sealing door 313 and the second sealing door is controlled so that the airflow flows from the extraction chamber 1 to the library construction chamber 2 in a single manner.

[0121] Experimental process pollution control description: Oil seal technology is to add mineral oil to all the reaction links in the PCR instrument to avoid aerosol cross contamination. Regular environmental monitoring process of the equipment In the extraction chamber 1 and the library chamber 2, after obtaining environmental samples by surface wiping sampling and air deposition sampling, the conventional sample detection process is used to monitor the pollution of the environment.

[0122] ① Oil sealing technology; in the fully automated process, mineral oil is added to the reaction links of all PCR instruments for liquid sealing to avoid aerosol contamination and prevent the reaction solution from evaporating at high temperatures, thereby improving the stability of the PCR reaction. The experiment designed two groups of controls: high-positive corporate reference products and negative samples were arranged alternately. One group added mineral oil to the process for testing, and the results showed that no positive pathogens were detected in the negative sample wells; the other group did not add mineral oil, and positive pathogens were detected in some negative sample wells. This shows that in the fully automated process, oil sealing technology can effectively prevent false positive results caused by aerosol contamination during the detection process. In the fully automated process of genetic testing, mineral oil is innovatively added to the reaction link of the PCR instrument for liquid sealing, and combined with a fully automated equipment system, aerosol contamination between test samples is effectively avoided.

[0123] ②Regular environmental monitoring process of the equipment: An environmental monitoring experiment was designed to regularly obtain environmental samples in the extraction chamber 1 and the library chamber 2 through surface wiping sampling and air deposition sampling, and then process them according to the fully automated process of clinical samples to monitor the pollution of the use environment. In the entire fully automated equipment system, an effective regular environmental monitoring process was innovatively introduced to ensure the reliability of the test results.

[0124] The present invention effectively solves the strict requirements of genetic testing on the experimental environment and experimenters by implementing a complete set of comprehensive solutions. In a conventional laboratory environment of about 8 square meters, a single person can complete the full process of automated genetic testing and generate a report. The cost of the library construction system 100 is reasonable and controllable, and the design meets the basic requirements of a standard molecular PCR laboratory. By physically partitioning the extraction and library construction links and connecting them through a transfer device 3, a rubber strip sealing design is adopted around the equipment, so that the extraction chamber 1 and the library construction chamber 2 can achieve a negative pressure difference of 5Pa, thereby effectively avoiding cross contamination. During the experiment, the necessary links innovatively adopted the "oil seal technology", and regularly conducted environmental pollution assessments to ensure the accuracy of the test results.

[0125] Example of system anti-pollution effect verification: In the oil seal technology, Solarbio M8040 mineral oil (paraffin oil) is used, which is a colorless and transparent liquid with a density of 0.84g / ml (25°C). It is stable to light, heat and acid, insoluble in water and ethanol, but soluble in volatile oils and most non-volatile oils. Its melting point is -24°C (literature value), boiling point is 300°C (literature value), and storage conditions are sealed at room temperature. This product is widely used as a matrix raw material for cosmetics and is used to cover samples in PCR reactions to prevent the reaction mixture from evaporating during the reaction.

[0126] In the library construction system 100 of this invention, the method of using mineral oil seal has significant advantages in detecting pathogenic products, and can effectively avoid cross contamination between samples of the same batch in the equipment, and play an isolation and stabilization role: Isolation: Cover the reaction system to form an oil film, block aerosol diffusion, and prevent sample cross contamination.

[0127] Stability: Protect the reaction system from the influence of external temperature and humidity, improve the efficiency and specificity of PCR reaction, and ensure the accuracy and reliability of test results.

[0128] Compatible: Compatible with automated equipment and does not affect subsequent detection processes, such as sequencing and data analysis.

[0129] A. Evaluation of the aerosol prevention and control effect of "oil seal technology": (1) Comparison of the experimental design of oil-sealed and non-oil-sealed automated gene detection: DNA was extracted from negative and high-positive corporate reference products, negative and positive quality control products in the kit, and blank control samples. The library was prepared using fully automated gene detection equipment according to the detection process of BGI's PTseq respiratory infection pathogen targeted high-throughput gene detection kit. Afterwards, sequencing was performed on the DNB sequencing device using the matching kit. Finally, the results were analyzed using a gene analysis all-in-one machine and a test report was issued. Two rounds of testing were performed: in the first round of control experiments, oil sealing was not performed, and the high-positive reference products and negative reference products were arranged alternately in a chessboard pattern for full-process testing; in the second round of experiments, the reaction system was oil-sealed in the reaction link of the PCR instrument, and the rest of the design was consistent with the first round. Standard equipment cleaning procedures were performed before and after each round of experiments. The results of the comparative analysis are shown in Table 1 below.

[0130] Table 1 Comparison of the detection results of high-throughput automated gene detection of pathogenic microorganisms with and without oil seals:

[0131] Note: The original detection results of the high positive reference products are Streptococcus pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, Candida albicans, and human gamma herpes virus type 4 (EBV). The concentrations of each pathogen in the positive reference products exceed 95% of the corresponding pathogen concentrations in patient samples in the intended use population. The original detection results of the positive control are Schizosaccharomyces pombe and MS2; the original detection results of the negative reference (HeLa cell matrix) and the negative control are negative.

[0132] (2) Results of automated gene detection with and without oil sealing: As shown in Table 1, when the high-throughput automated gene detection of pathogenic microorganisms was not oil-sealed, human gamma herpesvirus type 4 (EBV) was detected in two negative reference wells. However, when mineral oil seal was added during the reaction in the PCR instrument, no bacteria / virus was detected in the negative reference wells. Calculation of contamination ratio: When not oil-sealed, 3 reads of human gamma herpesvirus type 4 (EBV) were detected in the negative reference wells, and 2,622,493 reads of human gamma herpesvirus type 4 (EBV) were detected in the positive reference wells, with a contamination rate of 1 / 874,164; when oil-sealed, 0 reads of human gamma herpesvirus type 4 (EBV) were detected in the negative reference wells, and 11,707,887 reads of human gamma herpesvirus type 4 (EBV) were detected in the positive reference wells, with a contamination rate of less than 1 / 11,707,887. It is shown that the use of mineral oil seals in the detection of pathogenic products in this fully automated equipment can effectively avoid cross contamination between samples of the same batch in the equipment and ensure that the results of the detected samples are completely consistent with the expected results.

[0133] (3) Comparison of the experimental design of fully automated and manual testing: DNA was extracted from negative and high-positive corporate reference samples, negative and positive quality control samples in the kit, and blank control samples. The library was prepared using fully automated genetic testing equipment according to the detection process of the large gene PTseq respiratory pathogen targeted high-throughput gene detection kit. Afterwards, sequencing was performed on the DNB sequencing equipment using the matching kit. Finally, the results were analyzed using a gene analysis machine and a test report was issued. Two rounds of testing were conducted: the first round of control experiments used oil sealing technology to alternately arrange high-positive reference samples and negative reference samples in a chessboard pattern for full-process automated testing; the second round of experiments was completed in a standard high-throughput laboratory, and each step of the test was performed in the corresponding partition by laboratory personnel with work permits (i.e., the laboratory personnel completed the extraction and library construction operations on the extraction equipment in the sample extraction laboratory and the library construction equipment in the library construction laboratory, and finally used the sequencer and gene analysis machine to complete the sequencing and analysis work). Standard equipment cleaning procedures were performed before and after each round of experiments. The comparative analysis results are shown in Table 2 below.

[0134] Table 2 Comparison of results between high-throughput fully automated gene detection and manual detection of pathogenic microorganisms

[0135] Note: The original detection results of the high positive reference products are Streptococcus pneumoniae, Acinetobacter baumannii, Staphylococcus aureus, Candida albicans, and human gamma herpes virus type 4 (EBV). The concentrations of each pathogen in the positive reference products exceed 95% of the corresponding pathogen concentrations in patient samples in the intended use population. The original detection results of the positive control are Schizosaccharomyces pombe and MS2; the original detection results of the negative reference (HeLa cell matrix) and the negative control are negative.

[0136] (4) Comparison of the results of fully automated and manual testing: As shown in Table 2, in the high-throughput automated gene detection of pathogenic microorganisms (using oil sealing technology), no bacteria / viruses were detected in the negative reference wells. Contamination ratio calculation: 0 reads of human gamma herpesvirus type 4 (EBV) were detected in the negative reference wells, and 11,707,887 reads of human gamma herpesvirus type 4 (EBV) were detected in the positive reference wells, with a contamination rate of less than 1 / 11,707,887. In the manual detection results, 1 read of human gamma herpesvirus type 4 (EBV) was detected in the negative reference wells, and 2,319,337 reads of human gamma herpesvirus type 4 (EBV) were detected in the positive reference wells, with a contamination rate of 1 / 2,319,337; indicating that the overall effect of the automated system (including oil sealing technology) is better than the manual detection results under standard laboratory conditions.

[0137] B. Regular environmental monitoring and evaluation: Standard steps for equipment cleaning: After use, the cover and base of the PCR instrument need to be wiped once with a nucleic acid remover, then wiped once with ultrapure water, and finally wiped once with 75% ethanol. The software selects post-cleaning and irradiates with ultraviolet light for 15 to 30 minutes to complete the post-cleaning. Before using the equipment, wipe each table surface with ultrapure water, then wipe it again with 75% ethanol, the software selects pre-cleaning and irradiates with ultraviolet light for 15 to 30 minutes.

[0138] Regular environmental monitoring: Surface wiping and air deposition sampling monitoring are carried out once a month, and once every two weeks when the equipment is used frequently.

[0139] Surface wiping sampling: Sample processing & nucleic acid extraction area, place the sterile cotton swab in the ultrapure water sampling tube and soak it, gently wipe the magnetic head, hole position and workstation table (magnetic head, 6 holes are randomly selected, and the table is marked with an "S" shape), try to cover the main operation area, and after sampling, place the cotton swab in the sampling tube at a certain angle, break the cotton swab, and close the cover for inspection (No. A1); Library amplification / product detection area, place the sterile cotton swab in the ultrapure water sampling tube and soak it, gently wipe the workstation robotic arm, pipette tip and workstation table (6 holes are randomly selected from the table and an "S" shape is drawn), 6 holes are randomly selected from the PCR module, the bottom of the PCR cover and the bottom of the reagent cover, and after sampling, place the cotton swab in the sampling tube at a certain angle, break the cotton swab, and close the cover for inspection (No. A2).

[0140] Air deposition sampling method: Place one 2ml tube (numbered B1 and B2) containing 1mL of ultrapure water in each of the sample processing & nucleic acid extraction area and the library amplification / product detection area, open the tube cover, and place it on the countertop in the corresponding area of ​​the running equipment for 8 hours, or place it on the countertop in the corresponding area of ​​the equipment for 24 hours before the experiment (the equipment needs to be turned on to ensure the airflow direction); Detection method: The samples collected by the above sampling method (numbered A1, A2, B1, B2), 1 negative quality control, 1 weak positive quality control and 1 blank well are subjected to full-process automated testing.

[0141] Environmental monitoring results: Sample numbers A1, A2, B1, B2, negative quality control and blank wells were not detected, and the weak positive quality control detection result was Schizosaccharomyces pombe, MS2, which was consistent with expectations. The detection results of environmental monitoring samples showed that the environmental pollution in the warehouse of the fully automated equipment during operation was controllable.

[0142] In summary, the use of mineral oil seals to detect pathogenic products in this fully automated equipment can significantly reduce the risk of cross-contamination. At the same time, regular pollution detection is carried out during the entire process of equipment operation to ensure the controllability of environmental pollution and the accuracy of test results. It has important application value and promotion prospects.

[0143] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0144] In the description of the present invention, "first feature" or "second feature" may include one or more of the features.

[0145] In the description of the present invention, "plurality" means two or more.

[0146] In the description of the present invention, a first feature being “on” or “under” a second feature may include that the first and second features are directly in contact with each other, or may include that the first and second features are not in direct contact with each other but are in contact with each other via another feature therebetween.

[0147] In the description of the present invention, “on”, “over” and “above” a first feature from a second feature includes the first feature being directly above and obliquely above the second feature, or simply means that the first feature is horizontally higher than the second feature.

[0148] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0149] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A library construction system for gene detection, characterized in that: include: An extraction chamber, wherein an extraction device is installed in the extraction chamber, and the extraction device is used to extract a sample to be tested; A library building chamber, wherein a library building device is installed in the library building chamber, and the library building device is used to convert the extracted sample to be tested into an identifiable standardized library, and the extraction chamber and the library building chamber are arranged along a first direction and are spaced apart; A transfer device, the transfer device includes a transfer bin and a conveying mechanism, the transfer bin is arranged between the extraction bin and the library building bin, the conveying mechanism is penetrated through the transfer bin along the first direction, and one end of the conveying mechanism extends into the extraction bin, and the other end of the conveying mechanism extends into the library building bin, and the extraction bin and the library building bin can be selectively connected to the transfer bin, so that the conveying mechanism transports the sample to be tested in the extraction bin to the library building bin through the transfer bin.

2. The library construction system for gene detection according to claim 1, characterized in that: A first notch is formed on a first extraction chamber side wall opposite to the transfer chamber, and a second notch is formed on a first side wall of the transfer chamber opposite to the first extraction chamber side wall. The first notch and the second notch are opposite to and connected along the first direction, and the second notch is selectively opened or closed to connect or separate the extraction chamber and the transfer chamber, and when the second notch is opened, the conveying mechanism transports the sample to be tested to the transfer chamber through the first notch and the second notch.

3. The library construction system for gene detection according to claim 2, characterized in that: The first side wall is movably provided with a first sealing door, and the first sealing door is used to open or close the second gap.

4. The library construction system for gene detection according to claim 3, characterized in that: The first sealing door is slidably disposed on the first side wall along a second direction to open or close the second notch, and the first direction is perpendicular to the second direction.

5. The library construction system for gene detection according to claim 3, characterized in that: The first extraction bin side wall is also formed with a first through hole, the first through hole and the first notch are adjacent and connected along the second direction, the first side wall is formed with a second through hole, the second through hole and the second notch are adjacent and connected along the second direction, the first through hole and the second through hole are opposite and connected along the first direction, the conveying mechanism is penetrated through the first through hole and the second through hole, and the first direction is perpendicular to the second direction.

6. The library construction system for gene detection according to claim 5, characterized in that: The first sealing door has an abutting end, and a first sealing member is fixedly disposed on the abutting end. When the first sealing door closes the second notch, the first sealing member abuts and seals against the conveying mechanism.

7. The library construction system for gene detection according to claim 5, characterized in that: Along the third direction, the two conveying mechanism side walls of the conveying mechanism are both abutted and sealed against the transfer bin and the extraction bin, and the first direction, the second direction and the third direction are perpendicular to each other.

8. The library construction system for gene detection according to claim 2, characterized in that: A second seal is fixedly provided between the first side wall and the first extraction bin side wall, the second seal abuts against both the first side wall and the first extraction bin side wall, the second seal is arranged around the second notch along the circumference of the second notch, and the second seal is arranged around the first notch along the circumference of the first notch.

9. The library construction system for gene detection according to claim 8, characterized in that: A third seal is fixedly provided between the first side wall and the first extraction bin side wall, the third seal abuts against both the first side wall and the first extraction bin side wall, the third seal is located on the side of the second seal away from the first notch, and the third seal is arranged around the second seal along the circumference of the second seal.

10. The library construction system for gene detection according to claim 9, characterized in that: The third sealing member comprises a first sub-sealing strip, a second sub-sealing strip and a third sub-sealing strip, the second sub-sealing strip is connected between the first sub-sealing strip and the third sub-sealing strip, the first sub-sealing strip and the third sub-sealing strip both extend along the second direction and are respectively arranged on two side edges of the first side wall opposite to each other along the third direction, along the second direction, the second sub-sealing strip is arranged on an upper edge of the first side wall, and the first direction, the second direction and the third direction are perpendicular to each other.

11. The library construction system for gene detection according to claim 10, characterized in that: The third sealing member further comprises a fourth sub-sealing strip and a fifth sub-sealing strip. Along the third direction, the fourth sub-sealing strip and the fifth sub-sealing strip are respectively located on both sides of the conveying mechanism. Along the second direction, the lower end of the first sub-sealing strip is connected to the fourth sub-sealing strip, and the lower end of the third sub-sealing strip is connected to the fifth sub-sealing strip. Both the fourth sub-sealing strip and the fifth sub-sealing strip extend along the third direction and are arranged at the lower edge of the first side wall. The fourth sub-sealing strip and the fifth sub-sealing strip are respectively abutted and sealed against the two conveying mechanism side walls of the conveying mechanism.

12. The library construction system for gene detection according to any one of claims 2 to 11, characterized in that: The warehouse building warehouse has a first warehouse building warehouse side wall opposite to the transfer warehouse, and the transfer warehouse has a second side wall opposite to the first warehouse building warehouse side wall, the first warehouse building warehouse side wall and the first extraction warehouse side wall are constructed with the same structure, the second side wall and the first side wall are constructed with the same structure, and the assembly method of the first warehouse building warehouse side wall and the second side wall is the same as the assembly method of the first extraction warehouse side wall and the first side wall.

13. The library construction system for gene detection according to claim 11, characterized in that: At least one side wall of the extraction chamber is provided with a first chamber door, the first chamber door is used to open or close the extraction chamber, and / or At least one side wall of the warehouse building warehouse is provided with a second warehouse door, and the second warehouse door is used to open or close the warehouse building warehouse.

14. The library construction system for gene detection according to claim 13, characterized in that: The first door and the second door are identical.

15. The library construction system for gene detection according to claim 14, characterized in that: The first compartment door and the second compartment door both include: a door frame and a movable door, the door frame is formed with a taking-in and putting hole, and the movable door can be movably arranged on the door frame to open or close the taking-in and putting hole.

16. The library construction system for gene detection according to claim 15, characterized in that: The movable door and the door frame are arranged opposite to each other and are slidably arranged on the door frame along a second direction, the second direction is perpendicular to the first direction, a fourth seal is fixedly provided on the surface of the door frame facing the movable door, the fourth seal abuts against the movable door, a fifth seal is fixedly provided on the surface of the movable door facing the door frame, the fifth seal abuts against the door frame, and when the movable door closes the access hole, the fourth seal and the fifth seal are arranged around the access hole.

17. The library construction system for gene detection according to claim 16, characterized in that: The fourth sealing member comprises: a sixth sub-sealing strip and two seventh sub-sealing strips, wherein the sixth sub-sealing strip is located at an upper edge of the door frame along the second direction, the two seventh sub-sealing strips extend along the second direction and are respectively located at two side edges of the door frame, and the sixth sub-sealing strip is connected between the two fifth sub-sealing strips; The fifth sealing member includes: an eighth sub-sealing strip and two ninth sub-sealing strips, wherein the eighth sub-sealing strip is located at the lower edge of the movable door along the second direction, the two ninth sub-sealing strips extend along the second direction and are respectively located at two side edges of the movable door, the eighth sub-sealing strip is connected between the two ninth sub-sealing strips, and the seventh sub-sealing strip and the corresponding ninth sub-sealing strip are arranged along the width direction of the access hole.

18. The library construction system for gene detection according to any one of claims 1 to 11, characterized in that: The air pressure in the extraction chamber is greater than the air pressure in the storage chamber.

19. The library construction system for gene detection according to any one of claims 1 to 11, characterized in that: The air pressure in the transfer warehouse is lower than the air pressure in the extraction warehouse and higher than the air pressure in the storage warehouse.

20. The library construction system for gene detection according to any one of claims 1 to 11, characterized in that: The extraction chamber has a first gas drive component and a first filter element. The first gas drive component is used to drive gas to enter and flow out of the extraction chamber to adjust the gas pressure in the extraction chamber. The first filter element is used to filter the gas flowing into the extraction chamber.

21. The library construction system for gene detection according to any one of claims 1 to 11, characterized in that: The warehouse building warehouse has a second gas driving component and a second filter. The second gas driving component is used to drive gas to enter and flow out of the warehouse building warehouse to adjust the air pressure in the warehouse building warehouse. The second filter is used to filter the gas flowing into the warehouse building warehouse.

22. The library construction system for gene detection according to any one of claims 1 to 11, characterized in that: The conveying mechanism includes a driving mechanism, a guide rail and a placement table. The guide rail passes through the transfer bin along the first direction, and the two ends of the guide rail extend into the extraction bin and the library building bin respectively. The placement table is located outside the guide rail and is used to place the sample to be tested. At least a part of the driving mechanism is arranged in the guide rail and is transmission-connected to the placement table. The driving mechanism is used to drive the placement table to reciprocate relative to the guide rail along the first direction so that the placement table moves into one of the extraction bin, the library building bin and the transfer bin.

23. The library construction system for gene detection according to claim 22, characterized in that: The guide rail has a guide rail wall facing the placement table, and the guide rail wall is formed with an avoidance hole extending along the first direction. The driving mechanism includes a driving rod, and the driving rod is inserted into the avoidance hole. The avoidance hole is provided with a sixth sealing member, and the sixth sealing member is used to seal the avoidance hole. The sixth sealing member includes a first sealing strip and a second sealing strip, and the first sealing strip and the second sealing strip both extend along the first direction and are respectively located on both sides of the driving rod, and the first sealing strip and the second sealing strip both abut against the driving rod for sealing.

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