Automatic feeding device of bioreactor

Abstract

The invention discloses an automatic feeding device of a bioreactor, and relates to the technical field of bioreactors, the automatic feeding device comprises a bearing frame body, a reactor body is arranged on a frame body of the bearing frame body, and a vertical magnetic suspension linear guide rail is arranged on the surface of the side wall of the reactor body; under the cooperation of the directional material feeding assembly, the magnetic drive non-contact valve and the vertical magnetic suspension linear guide rail, the whole reactor can be adjusted in orientation and angle in the reactor body according to the lamination gradient of different reactants, and trace and accurate material feeding can be realized, so that the material feeding position is more accurate, and the material feeding efficiency is improved. In the complex biological reaction process, the uniformity and stability of biological reaction are improved, the feeding reaction is flexibly and actively adjusted, the change in the biological reaction process is quickly responded, reaction failure or product quality reduction caused by out-of-control parameters is avoided, and diversified production requirements are met.

Description

Technical Field

[0001] The invention relates to the technical field of bioreactors, in particular to an automatic feeding device for a bioreactor. Background Art

[0002] At present, a bioreactor refers to a reaction system that uses naturally occurring microorganisms or microorganisms with special degradation capabilities to inoculate into a liquid or solid phase. In the process of continuous biological fermentation, it is often necessary to feed the reactor every day or every other day. These feeds include sludge, high-concentration sewage, livestock and poultry manure, etc. At the same time, bioreactors are widely used in biopharmaceuticals, food processing, environmental engineering and other fields to cultivate microorganisms, cells or enzymes to produce target products. The automatic feeding device is a key component of the bioreactor, responsible for accurately controlling the feeding of nutrients, substrates or other additives to ensure the stable progress of the biological process in the reactor.

[0003] At present, traditional automatic feeding devices usually rely on simple timers, flow meters or sensors to control feeding. Although these devices can achieve basic automation, they still have the following problems in actual use: traditional devices are difficult to cope with complex biological reaction processes, and the feed amount control is not precise enough, which may cause fluctuations in nutrient concentrations in the reactor and affect product quality and output. Different biological reaction processes have different requirements for feeding, and traditional devices are difficult to flexibly adjust feeding strategies and cannot adapt to diverse production needs. Therefore, it is necessary to propose an automatic feeding device for bioreactors. Summary of the invention

[0004] The purpose of the present invention is to provide an automatic feeding device for a bioreactor to solve the problem proposed in the above background technology that when in use, traditional devices are difficult to cope with complex biological reaction processes, and the feed amount control is not precise enough, which may cause fluctuations in the concentration of nutrient substances in the reactor and affect the quality and output of the product. Among them, different biological reaction processes have different requirements for feed, and traditional devices are difficult to flexibly adjust the feeding strategy and cannot adapt to diversified production needs.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an automatic feeding device for a bioreactor, comprising a supporting frame, a reactor body is mounted on the frame of the supporting frame, a vertical magnetic suspension linear guide is mounted on the side wall surface of the reactor body, a plurality of groups of magnetically driven contactless valves are mounted on the surface of the vertical magnetic suspension linear guide, a sliding position seat is slidably connected on the surface of the vertical magnetic suspension linear guide, a double guide slide is fastened and connected to the side end surface of the supporting frame, a feeding automatic control component is slidably connected to the outside of the double guide slide, a directional material dosing component is mounted inside the reactor body, and an integrated online sensor group is mounted on the inner surface of the reactor body; The feeding automatic control component includes a variable frequency control motor, the side end of the variable frequency control motor is connected to a barrel through a transmission, the top end of the barrel is provided with a feeding end, the feeding end is connected to an external multi-channel storage tank, an in-situ hydrogen peroxide sterilizer is installed on the top wall surface of the barrel, and a metering guide is installed on the bottom of the side end of the barrel; The directional material feeding assembly includes an annular flexible guide rail, the bottom end of the annular flexible guide rail is rotatably connected to an annular material pipe, the side end of the annular material pipe is connected to a rotary valve, the side end of the rotary valve is connected to a metering feeder, the top wall surface of the annular material pipe is connected to a material delivery pipe, and the top end of the material delivery pipe is connected to a sliding hollow micro pumping seat.

[0006] Preferably, a sliding link is installed at the bottom of the barrel, and the sliding link is located in an external sliding connection of a double guide slide column. An electromagnetic guide rod is installed at the side end of the sliding link, and the side end of the electromagnetic guide rod is inserted through a metering guide and is connected to a pushing plate. A discharge valve is installed at the bottom of the metering guide, and the side end surface of the metering guide is connected to an automatic docking guide groove, and a spiral pushing structure is connected to the output end of the frequency conversion control motor.

[0007] Preferably, the side end of the sliding hollow micro pumping seat is fastened to a shell, a connecting frame plate is installed inside the shell, a small driving servo motor is installed at the top and bottom ends of the connecting frame plate, and the output end of the small driving servo motor is connected to small rotating teeth, and the small rotating teeth are arranged in two groups, and there is a distance difference between the two groups of small rotating teeth.

[0008] Preferably, the bottom ends of one group of the small rotating teeth are meshedly connected with the inner rotating teeth, and the top ends of the other group of the small rotating teeth are meshedly connected with the outer rotating teeth, the side ends of the inner rotating teeth are connected with the inner rotating rod, the side ends of the outer rotating teeth are connected with the outer connecting rod, and the side ends of the inner rotating rod are connected with the first bevel gear.

[0009] Preferably, the bottom end of the first bevel gear is meshingly connected with the second bevel gear, the top end of the second bevel gear is connected to the surface of the external connecting rod, the surface of the shell is rotatably connected with the stator and rotor structure, the bottom end of the second bevel gear is connected to a metering feeding conduit, the top of the metering feeding conduit is connected to a microfluidic chip feeding rack end, and the side end of the microfluidic chip feeding rack end is connected to a sliding hollow micro pumping seat through a flexible telescopic conduit.

[0010] Preferably, surfaces of the two groups of small rotating teeth, inner rotating teeth, outer rotating teeth, first bevel gear and second bevel gear are all coated with antibacterial coating.

[0011] Preferably, the bottom of the reactor body is connected to an exhaust valve tube, the side end of the exhaust valve tube is connected to a micro worm gear, the side end of the micro worm gear and an external piezoelectric ceramic pump are connected in a closed loop through a line, and the external piezoelectric ceramic pump is used to form a connection with an external multi-channel storage tank.

[0012] Preferably, the top of the reactor body is connected with a cleaning connecting mist pipe, the cleaning connecting mist pipe is connected to an external cleaning mister, and the bottom side end of the reactor body is connected with a discharge guide valve pipe.

[0013] Preferably, a fine-tuning cylinder is installed on the side end of the sliding position seat, and a docking feed valve end is slidably connected on the surface of the sliding position seat.

[0014] Preferably, the output end of the fine-tuning cylinder is connected to the end of the feed valve, and laser position sensors are installed on the top and bottom ends of the sliding position seat.

[0015] Compared with the prior art, the present invention has the following beneficial effects: In the present invention, by cooperating with the directional material feeding component, the magnetically driven contactless valve and the vertical magnetic suspension linear guide rail, the whole can be adjusted in azimuth and angle according to the layered gradient of different reactants inside the reactor body, so that the metering feeding conduit can accurately deliver the material to the required position according to the parameter changes in different areas inside the reactor body monitored by the sensor group, such as uneven distribution of biomass, differences in substrate concentration at different depths, etc. For example, when culturing specific microorganisms in the reactor body, the microorganisms in the bottom area may have different requirements for nutrients from those in the upper area due to different growth environments and metabolic activities, thereby enabling the metering feeding conduit to be adjusted in azimuth and angle. , accurately deliver the right amount of nutrient materials to the bottom area to meet the growth needs of microorganisms, improve the efficiency of biological reactions and product quality, and the microfluidic chip delivery rack end is connected to the sliding hollow micro pumping seat, which is convenient for the microfluidic chip to perform micro-quantity processing and distribution of materials, so that the metering feeding catheter can not only control the delivery position when delivering materials, but also realize micro-amount and precise material delivery, making the material delivery position more accurate, which is beneficial to improve the uniformity and stability of biological reactions in complex biological reaction processes, flexibly and actively adjust the feeding reaction, quickly respond to changes in the biological reaction process, avoid reaction failure or product quality degradation due to parameter loss of control, and adapt to diversified production needs.

[0016] 2. In the present invention, with the cooperation of the automatic feeding control component, when the integrated online sensor group monitors that the parameters in the reactor body deviate from the preset range, such as the decrease in substrate concentration, the change in pH value, etc., the automatic feeding control component is triggered to start, that is, the frequency conversion control motor starts to work, and its output end drives the spiral pushing structure to rotate, and the spiral pushing structure pushes the material in the barrel along the barrel wall to the bottom. When the material is pushed to the metering guide, the metering guide accurately meters the material according to the preset feed amount. At this time, the electromagnetic guide rod pushes the push plate after receiving the instruction of the external PLC controller, and pushes the measured material to the automatic docking guide groove through the discharge valve. The automatic docking guide groove is connected with the magnetically driven contactless valve on the sliding position seat and the vertical magnetic suspension linear guide rail. The door cooperates to transport the material to the metering feeder, and the sliding link slides on the double guide slide column at the same time. By adjusting its own position, the automatic feed control component can align with the magnetic-driven contactless valve in different positions to realize the transportation of materials in different heights or areas, and the extension and retraction of the electromagnetic guide rod can also flexibly control the position and pushing force of the push plate according to actual needs to ensure the accurate transportation of materials. By setting an in-situ hydrogen peroxide sterilizer as a whole, disinfection is carried out in real time during the material storage and transportation process to prevent microbial contamination of materials, thereby improving production efficiency, ensuring the sterile environment in the bioreactor, reducing the risk of biological reaction failure, and being able to accurately adjust the feed amount according to the real-time reaction situation in the reactor body under the detection of the integrated online sensor group. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic structural diagram of the main view of an automatic feeding device of a bioreactor according to the present invention; Figure 2 It is a schematic structural diagram of a side view of an automatic feeding device of a bioreactor of the present invention; Figure 3 It is a structural schematic diagram of an automatic feeding control component in an automatic feeding device of a bioreactor according to the present invention; Figure 4 It is a schematic diagram of the installation position structure of a sliding position seat, a laser position sensor, a fine-tuning cylinder and a docking feed valve end in an automatic feed device of a bioreactor of the present invention; Figure 5 This is a schematic diagram of the installation position structure of a directional material dosing component in an automatic feeding device of a bioreactor according to the present invention; Figure 6 It is a structural schematic diagram of a directional material dosing component in an automatic feeding device of a bioreactor of the present invention; Figure 7 The present invention is a partial structural schematic diagram of a directional material dosing component in an automatic feeding device of a bioreactor.

[0018] In the figure: 1. Carrying frame; 2. Reactor body; 3. Cleaning connecting mist pipe; 4. Magnetic drive non-contact valve; 5. Vertical magnetic suspension linear guide rail; 6. Exhaust valve pipe; 7. Micro worm gear; 8. Discharge guide valve pipe; 9. Feed automatic control component; 91. Frequency conversion control motor; 92. Barrel; 93. In-situ hydrogen peroxide sterilizer; 94. Feed end; 95. Spiral pusher structure; 96. Sliding link; 97. Metering guide; 98. Electromagnetic guide rod; 99. Pusher plate; 990. Discharge valve; 991. Automatic docking guide groove; 10. Double guide slide column; 11. Sliding position seat; 12. Laser position sensor; 1 3. Fine-tuning cylinder; 14. Docking feed valve end; 15. Directional material feeding assembly; 150. Annular flexible guide rail; 151. Rotary valve; 152. Metering feeder; 153. Annular material pipe; 154. Sliding hollow micro pumping seat; 155. Feed pipe; 156. Connecting frame plate; 157. Small driving servo motor; 158. Small rotating gear; 159. Internal rotating gear; 1590. External rotating gear; 1591. Stator and rotor structure; 1592. External connecting rod; 1593. Internal rotating rod; 1595. First bevel gear; 1596. Second bevel gear; 1597. Microfluidic chip feeding rack end; 1598. Metering feeding conduit. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the implementation regulations described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Example 1: Reference Figure 1 - Figure 7 As shown: an automatic feeding device for a bioreactor, comprising a supporting frame 1, a reactor body 2 is installed on the frame of the supporting frame 1, a vertical magnetic suspension linear guide 5 is installed on the side wall surface of the reactor body 2, a plurality of groups of magnetically driven contactless valves 4 are installed on the surface of the vertical magnetic suspension linear guide 5, a sliding position seat 11 is slidably connected on the surface of the vertical magnetic suspension linear guide 5, a double guide slide 10 is fastened and connected on the side end surface of the supporting frame 1, a feeding automatic control component 9 is slidably connected on the outside of the double guide slide 10, a directional material dosing component 15 is installed inside the reactor body 2, and an integrated online sensor group is installed on the inner surface of the reactor body 2; The directional material feeding assembly 15 includes an annular flexible guide rail 150, the bottom end of the annular flexible guide rail 150 is rotatably connected to an annular material pipe 153, the side end of the annular material pipe 153 is connected to a rotary valve 151, the side end of the rotary valve 151 is connected to a metering feeder 152, the top wall surface of the annular material pipe 153 is connected to a feed pipe 155, and the top end of the feed pipe 155 is connected to a sliding hollow micro pumping seat 154.

[0021] The side end of the sliding hollow micro pumping seat 154 is fastened to the shell, and a connecting frame plate 156 is installed inside the shell. A small driving servo motor 157 is installed at the top and bottom ends of the connecting frame plate 156. The output end of the small driving servo motor 157 is connected to a small rotating gear 158. The small rotating gear 158 is arranged in two groups, and there is a distance difference between the two groups of small rotating gears 158.

[0022] The bottom end of one group of small rotating teeth 158 is meshedly connected with the inner rotating teeth 159, and the top end of the other group of small rotating teeth 158 is meshedly connected with the outer rotating teeth 1590. The side end of the inner rotating teeth 159 is connected with the inner rotating rod 1593, the side end of the outer rotating teeth 1590 is connected with the outer connecting rod 1592, and the side end of the inner rotating rod 1593 is connected with the first bevel gear 1595.

[0023] The bottom end of the first bevel gear 1595 is meshingly connected to the second bevel gear 1596, the top end of the second bevel gear 1596 is connected to the surface of the external connecting rod 1592, the surface of the shell is rotatably connected to the stator and rotor structure 1591, the bottom end of the second bevel gear 1596 is connected to the metering feeding conduit 1598, the top of the metering feeding conduit 1598 is connected to the microfluidic chip delivery rack end 1597, and the side end of the microfluidic chip delivery rack end 1597 is connected to the sliding hollow micro pumping seat 154 through a flexible telescopic conduit.

[0024] The surfaces of the two groups of small rotating teeth 158 , the inner rotating teeth 159 , the outer rotating teeth 1590 , the first bevel gear 1595 and the second bevel gear 1596 are all coated with an antibacterial coating.

[0025] In a specific solution, the support frame 1 provides stable support for the entire device, and then the reactor body 2 is installed thereon, and the vertical magnetic suspension linear guide rail 5 is installed on the side wall of the reactor body 2. The magnetic suspension technology is used to reduce friction and achieve high-precision linear motion. A plurality of sets of magnetically driven contactless valves 4 are installed on the surface of the vertical magnetic suspension linear guide rail 5, so that the opening and closing are controlled by magnetic drive without mechanical contact, thereby reducing the risk of wear and contamination. The sliding position seat 11 can slide freely on the vertical magnetic suspension linear guide rail 5 for positioning and installing other structures. The double guide slide column 10 is fixed to the side end of the support frame 1 to provide stable guidance for the automatic feeding control component 9 to ensure its accuracy and stability during the up and down movement. The integrated online sensor group monitors various parameters inside the reactor body 2 in real time, such as temperature, pH value, dissolved oxygen, biomass, etc., to provide data support for subsequent feeding control. When the integrated online sensor group detects that the parameters in the reactor body 2 deviate from the preset range, such as the substrate concentration decreases, the pH value changes, etc., the feeding automatic control component 9 is started. The feeding automatic control component 9 moves to a suitable position along the double guide slide column 10 according to the sensor data, and cooperates with the magnetically driven contactless valve 4 on the vertical magnetic suspension linear guide rail 5 through the sliding position seat 11 to control the entry path of the material. After the material is accurately measured and distributed by the directional material feeding component 15, it is transported to the reactor body 2 to complete the automatic feeding process. At the same time, The line sensor group collects data in real time and transmits it to the external PLC controller through AI. The external PLC controller analyzes the data through the AI ​​algorithm and determines the current reaction state. If it is detected that the nutrient concentration in a certain area is insufficient, the external PLC controller drives the vertical magnetic suspension linear guide rail 5 to move the sliding position seat 11 to the target area, and controls the opening of the feed pipe 155 through the magnetic drive non-contact valve 4. At the same time, the annular flexible guide rail 150 is installed inside the reactor body 2, and the bottom end thereof is internally rotated to connect the annular material pipe 153 so that the annular material pipe 153 can rotate around the central axis of the annular flexible guide rail 150. The annular material pipe 153 is used to store and transport materials, and its side end is connected to the rotary valve 151 and the metering feeder 152. The metering feeder 152 is connected to the feeder 155. The material is accurately measured according to the preset feed amount, and then the measured material is conveyed to the annular material pipe 153 through the rotary valve 151. The conveying pipe 155 connected to the top wall surface of the annular material pipe 153 conveys the material to the sliding hollow micro pumping seat 154. The small driving servo motors 157 on the top and bottom of the side of the sliding hollow micro pumping seat 154 are started respectively, and the output ends thereof drive the small rotating teeth 158 to rotate. The two groups of small rotating teeth 158 are respectively meshed with the inner rotating teeth 159 and the outer rotating teeth 1590. Since there is a distance difference between the two groups of small rotating teeth 158, when one group or the other group of small rotating teeth 158 rotates, the inner rotating teeth 159 and the outer rotating teeth 1590 can be driven to rotate in different directions respectively, and the inner rotating teeth 159 drive the inner rotating rod 1593 to rotate.The inner rotating rod 1593 is connected to the first bevel gear 1595, so that the first bevel gear 1595 rotates, the first bevel gear 1595 meshes with the second bevel gear 1596, and the power is transmitted to the second bevel gear 1596. The outer rotating gear 1590 drives the outer connecting rod 1592 to rotate, and the second bevel gear 1596 is connected to the outer connecting rod 1592 to form a rotation adjustment. At the same time, the bottom end of the second bevel gear 1596 is connected to the metering feeding conduit 1598. Driven by the second bevel gear 1596, the metering feeding conduit 1598 rotates, thereby realizing The quantitative delivery of materials is realized, and the microfluidic chip delivery rack end 1597 is connected to the sliding hollow micro pumping seat 154 through a flexible telescopic catheter. The microfluidic chip can process and distribute the materials more finely, realize the micro-amount and precise delivery of materials, and the whole can form an adjustment in direction and angle according to the layered gradient of different reactants inside the reactor body 2, so that the metering feeding conduit 1598 can adjust the parameters of different areas inside the reactor body 2 monitored by the sensor group, such as uneven distribution of biomass, substrate concentration at different depths, etc. Differences, etc., can accurately deliver materials to the required position. For example, when cultivating specific microorganisms in the reactor body 2, the microorganisms in the bottom area may have different requirements for nutrients from those in the upper area due to different growth environments and metabolic activities. As a result, the metering feeding conduit 1598 can accurately deliver the appropriate amount of nutrients to the bottom area by adjusting the orientation and angle to meet the growth needs of microorganisms and improve the efficiency and product quality of the biological reaction. In addition, the microfluidic chip delivery rack end 1597 is connected to the sliding hollow micro pumping seat 154, which is convenient for the microfluidic chip to perform micro-quantity processing and distribution of materials. When the metering feeding conduit 1598 delivers materials, it can not only control the delivery position, but also realize micro-amount and precise delivery of materials, making the material delivery position more accurate, which is beneficial to improve the uniformity and stability of the biological reaction in the complex biological reaction process, flexibly and actively adjust the feeding reaction, quickly respond to changes in the biological reaction process, avoid reaction failure or product quality degradation due to parameter loss of control, and adapt to diversified production needs.

[0026] Embodiment 2: According to Figure 1 - Figure 3 As shown, the automatic feeding control component 9 includes a variable frequency controlled motor 91, the side end of the variable frequency controlled motor 91 is connected to a barrel 92 through a transmission, a feeding end 94 is opened at the top end of the barrel 92, and the feeding end 94 is connected to an external multi-channel storage tank. An in-situ hydrogen peroxide sterilizer 93 is installed on the top wall surface of the barrel 92, and a metering guide 97 is installed on the bottom of the side end of the barrel 92.

[0027] A sliding link 96 is installed at the bottom of the barrel 92, and the sliding link 96 is located in the external sliding connection of the double guide slide column 10. An electromagnetic guide rod 98 is installed at the side end of the sliding link 96. The side end of the electromagnetic guide rod 98 is inserted through the metering guide 97 and is connected to a pushing plate 99. A discharge valve 990 is installed at the bottom of the metering guide 97. The side end surface of the metering guide 97 is connected to an automatic docking guide groove 991, and the output end of the frequency conversion control motor 91 is connected to a spiral pushing structure 95.

[0028] In a specific solution, an external multi-channel storage tank is connected to the barrel 92 through a feed end 94, and different types of materials are transported into the barrel 92, while an in-situ hydrogen peroxide sterilizer 93 disinfects and sterilizes the internal space of the barrel 92 and the materials to ensure that the materials entering the bioreactor are in a sterile state, providing a pure environment for subsequent biological reactions. When the integrated online sensor group detects that the parameters in the reactor body 2 deviate from the preset range, such as a decrease in substrate concentration, a change in pH value, etc., the automatic feed control component 9 is triggered to start, that is, the variable frequency control motor 91 starts to work, and its output end drives the spiral pusher structure 95 to rotate. The spiral pusher structure 95 pushes the material in the barrel 92 along the barrel wall to the bottom. When the material is pushed to the metering guide 97, the metering guide 97 accurately meters the material according to the preset feed amount. At this time, the electromagnetic guide rod 98 pushes the pusher plate 99 after receiving the instruction of the external PLC controller, and the measured material passes through the discharge valve 99 0 is pushed to the automatic docking guide groove 991, and the automatic docking guide groove 991 cooperates with the sliding position seat 11 and the magnetically driven contactless valve 4 on the vertical magnetic suspension linear guide rail 5 to transport the material to the metering feeder 152. At the same time, the sliding link 96 slides on the double guide slide column 10, and by adjusting its own position, the feeding automatic control component 9 can be aligned with the magnetically driven contactless valve 4 at different positions to realize the transportation of materials at different heights or areas, and the extension and contraction of the electromagnetic guide rod 98 can also flexibly control the position and pushing force of the push plate 99 according to actual needs to ensure the accurate transportation of materials. By setting an in-situ hydrogen peroxide sterilizer 93 as a whole, disinfection is carried out in real time during the material storage and transportation process to prevent microbial contamination of materials, thereby improving production efficiency, while ensuring the sterile environment in the bioreactor, reducing the risk of biological reaction failure, and being able to accurately adjust the feed amount according to the real-time reaction situation in the reactor body 2 under the detection of the integrated online sensor group.

[0029] Embodiment 3: According to Figure 1 , Figure 2 , Figure 4 and Figure 5As shown, the bottom of the reactor body 2 is connected to an exhaust valve pipe 6, the side end of the exhaust valve pipe 6 is connected to a micro worm gear 7, the side end of the micro worm gear 7 and an external piezoelectric ceramic pump are connected in a closed loop through a line, and the external piezoelectric ceramic pump is used to form a connection with an external multi-channel storage tank.

[0030] The top of the reactor body 2 is connected with a cleaning connecting mist pipe 3 , and the cleaning connecting mist pipe 3 is connected with an external cleaning mister. The bottom side of the reactor body 2 is connected with a discharge guide valve pipe 8 .

[0031] A fine-tuning cylinder 13 is installed at the side end of the sliding position seat 11 , and a butt feed valve end 14 is slidably connected on the surface of the sliding position seat 11 .

[0032] The output end of the fine-tuning cylinder 13 is connected to the feed valve end 14 , and the top and bottom ends of the sliding position seat 11 are both installed with laser position sensors 12 .

[0033] In a specific scheme, when the pressure in the reactor body 2 reaches a certain threshold, the exhaust valve pipe 6 opens, causing the micro worm gear 7 to rotate under the action of the gas flow, and its rotation signal is transmitted to the external piezoelectric ceramic pump through the line. The external piezoelectric ceramic pump adjusts the working state according to the signal, extracts the gas from the reactor body 2, and maintains the pressure balance in the reactor body 2. At the same time, the external piezoelectric ceramic pump can also process or recycle the extracted gas. When a biological reaction is completed or the reactor body 2 needs to be maintained, the cleaning operation is started, and the external cleaning mister sprays the cleaning liquid mist into the reactor body 2 through the cleaning connecting mist pipe 3, so that the cleaning liquid mist can evenly cover every corner inside the reactor, and the inner wall and internal components of the reactor body 2 are cleaned. Cleaning is performed to remove residual materials, biological residues, etc., to ensure the cleanliness of the reactor and prepare for the next reaction. When the biological reaction is completed and the reaction product needs to be discharged, the discharge guide valve pipe 8 is opened, and the reaction product is discharged from the reactor body 2 through the discharge guide valve pipe 8 and enters the subsequent processing link. When the above-mentioned sliding position seat 11 slides on the vertical magnetic suspension linear guide rail 5, its position is monitored in real time by the laser position sensor 12 to ensure that it reaches the target position accurately, so that the fine-tuning cylinder 13 fine-tunes the position of the docking feed valve end 14 according to the feedback information of the laser position sensor 12, so that it is accurately docked with the magnetically driven contactless valve 4, thereby facilitating the material to enter the reactor body 2 through the docking feed valve end 14 and the magnetically driven contactless valve 4.

[0034] The wiring diagram of the in-situ hydrogen peroxide sterilizer 93, the laser position sensor 12, the small drive servo motor 157 and the microfluidic chip delivery rack end 1597 in the present invention is common knowledge in the field, and its working principle is a well-known technology. The model is selected according to the actual use. Therefore, the control method and wiring layout of the in-situ hydrogen peroxide sterilizer 93, the laser position sensor 12, the small drive servo motor 157 and the microfluidic chip delivery rack end 1597 will not be explained in detail.

[0035] The method of using the device and the working principle are as follows: first, the integrated online sensor group monitors various parameters inside the reactor body 2 in real time, such as temperature, pH value, dissolved oxygen, biomass, etc., to provide data support for subsequent feeding control. When the integrated online sensor group detects that the parameters in the reactor body 2 deviate from the preset range, such as the substrate concentration decreases, the pH value changes, etc., the feeding automatic control component 9 is started, and the feeding automatic control component 9 moves to a suitable position along the double guide slide column 10 according to the sensor data, and uses the laser position sensor 12 to monitor its position in real time to ensure that it reaches the target position accurately, so that the fine-tuning cylinder 13 drives the magnetic force drive on the vertical magnetic suspension linear guide 5 through the sliding position seat 11 according to the feedback information of the laser position sensor 12. The contact valve 4 cooperates to control the entry path of the material, so that the external multi-channel storage tank is connected to the barrel 92 through the feed end 94, and different types of materials are transported into the barrel 92, and the in-situ hydrogen peroxide sterilizer 93 disinfects and sterilizes the internal space of the barrel 92 and the materials to ensure that the materials entering the bioreactor are in a sterile state, providing a pure environment for subsequent biological reactions. When the integrated online sensor group monitors that the parameters in the reactor body 2 deviate from the preset range, such as the decrease in substrate concentration, the change in pH value, etc., the feed automatic control component 9 is triggered to start, that is, the frequency conversion control motor 91 starts to work, and its output end drives the spiral pusher structure 95 to rotate, and the spiral pusher structure 95 pushes the material in the barrel 92 along the barrel wall to the bottom, and the material is pushed to the metering When the guide 97 is moved, the metering guide 97 accurately meters the material according to the preset feed amount. At this time, the electromagnetic guide rod 98 pushes the push plate 99 after receiving the command of the external PLC controller, and pushes the measured material to the automatic docking guide groove 991 through the discharge valve 990. The automatic docking guide groove 991 cooperates with the sliding position seat 11 and the magnetically driven non-contact valve 4 on the vertical magnetic suspension linear guide rail 5 to transport the material to the metering feeder 152. At the same time, the sliding link 96 slides on the double guide slide column 10. By adjusting its own position, the automatic feed control component 9 can align with the magnetically driven non-contact valve 4 at different positions to realize the transportation of materials at different heights or areas, and the extension and retraction of the electromagnetic guide rod 98 can also be adjusted according to actual needs. , flexibly control the position and pushing force of the push plate 99 to ensure accurate material transportation. After the material is accurately measured and distributed by the directional material feeding component 15, it is transported to the reactor body 2 to complete the automatic feeding process. At the same time, the online sensor group collects data in real time and transmits it to the external PLC controller through AI. The external PLC controller analyzes the data through the AI ​​algorithm and determines the current reaction state. If it is detected that the nutrient concentration in a certain area is insufficient, the external PLC controller drives the vertical magnetic suspension linear guide rail 5 to move the sliding position seat 11 to the target area, and controls the opening of the feed pipe 155 through the magnetic drive non-contact valve 4. At the same time, the annular flexible guide rail 150 is installed inside the reactor body 2, and its bottom end is internally rotated to connect the annular material pipe 153.The annular material pipe 153 can rotate around the central axis of the annular flexible guide rail 150. The annular material pipe 153 is used to store and transport materials. The side end of the annular material pipe 153 is connected to the rotary valve 151 and the metering feeder 152. The metering feeder 152 accurately meters the material according to the preset feed amount, and then transports the metered material to the annular material pipe 153 through the rotary valve 151. The feeding pipe 155 connected to the top wall surface of the annular material pipe 153 transports the material to the sliding hollow micro pumping seat 154. The small driving servo motors 157 on the top and bottom of the side of the sliding hollow micro pumping seat 154 are started respectively, and the output end thereof drives the small rotating gear 158 to rotate. The two groups of small rotating gears 158 are respectively engaged with the inner rotating gear 159 and the outer rotating gear 1590. There is a distance difference between the teeth 158. When one group or the other group of small rotating teeth 158 rotates, the inner rotating teeth 159 and the outer rotating teeth 1590 can be driven to rotate in different directions respectively. The inner rotating teeth 159 drive the inner rotating rod 1593 to rotate. The inner rotating rod 1593 is connected to the first bevel gear 1595, so that the first bevel gear 1595 rotates. The first bevel gear 1595 is meshed with the second bevel gear 1596 to transmit power to the second bevel gear 1596. The outer rotating teeth 1590 drive the outer connecting rod 1592 to rotate. The second bevel gear 1596 is connected to the outer connecting rod 1592 to form a rotation adjustment. At the same time, the bottom end of the second bevel gear 1596 is connected to the metering feeding conduit 1598. Driven by the second bevel gear 1596, the metering feeding conduit 1598 is fed into the feed tube 1598. The microfluidic chip feeding rack end 1597 is connected to the sliding hollow micro pumping seat 154 through a flexible telescopic catheter. The microfluidic chip can process and distribute the material more finely to achieve trace and accurate material feeding. The whole can form azimuth and angle adjustments according to the layered gradient of different reactants inside the reactor body 2, so that the metering feeding conduit 1598 can accurately deliver the material to the required position according to the parameter changes in different areas of the reactor body 2 monitored by the sensor group, such as uneven distribution of biomass and differences in substrate concentration at different depths. For example, when cultivating specific microorganisms in the reactor body 2, the microorganisms in the bottom area may have different growth environments and metabolic activities. , the demand for nutrients is different from that in the upper area, so that the metering feed conduit 1598 can accurately deliver the right amount of nutrients to the bottom area by adjusting the direction and angle to meet the growth needs of microorganisms. When the pressure in the reactor body 2 reaches a certain threshold, the exhaust valve pipe 6 opens, so that the micro worm gear 7 rotates under the action of gas flow, and its rotation signal is transmitted to the external piezoelectric ceramic pump through the line. The external piezoelectric ceramic pump adjusts the working state according to the signal, extracts the gas from the reactor body 2, and maintains the pressure balance in the reactor body 2. At the same time, the external piezoelectric ceramic pump can also process or recycle the extracted gas. When a biological reaction is completed or the reactor body 2 needs to be maintained, the cleaning operation is started.The external cleaning mist sprays the cleaning liquid mist into the reactor body 2 through the cleaning connecting mist pipe 3, so that the cleaning liquid mist can evenly cover every corner inside the reactor, clean the inner wall and internal components of the reactor body 2, remove residual materials, biological residues, etc., ensure the cleanliness of the reactor, and prepare for the next reaction. When the biological reaction is over and the reaction product needs to be discharged, the discharge guide valve pipe 8 is opened, and the reaction product is discharged from the reactor body 2 through the discharge guide valve pipe 8 and enters the subsequent processing link.

[0036] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An automatic feeding device for a bioreactor, characterized in that: The invention comprises a supporting frame (1), a reactor body (2) is mounted on the supporting frame (1), a vertical magnetic suspension linear guide rail (5) is mounted on the side wall surface of the reactor body (2), a plurality of groups of magnetically driven contactless valves (4) are mounted on the surface of the vertical magnetic suspension linear guide rail (5), a sliding position seat (11) is slidably connected on the surface of the vertical magnetic suspension linear guide rail (5), a double guide slide column (10) is fastened to the side end surface of the supporting frame (1), an automatic feed control component (9) is slidably connected to the outside of the double guide slide column (10), a directional material dosing component (15) is mounted inside the reactor body (2), and an integrated online sensor group is mounted on the inner surface of the reactor body (2); The feeding automatic control component (9) comprises a variable frequency control motor (91), the side end of the variable frequency control motor (91) is connected to a barrel (92) via a transmission, the top end of the barrel (92) is provided with a feeding end (94), the feeding end (94) is connected to an external multi-channel storage tank, an in-situ hydrogen peroxide sterilizer (93) is installed on the top wall surface of the barrel (92), and a metering guide (97) is installed at the bottom of the side end of the barrel (92); The directional material feeding assembly (15) comprises an annular flexible guide rail (150), the bottom end of the annular flexible guide rail (150) is rotatably connected to an annular material pipe (153), the side end of the annular material pipe (153) is connected to a rotary valve (151), the side end of the rotary valve (151) is connected to a metering feeder (152), the top wall surface of the annular material pipe (153) is connected to a material delivery pipe (155), and the top end of the material delivery pipe (155) is connected to a sliding hollow micro pumping seat (154).

2. The automatic feeding device for a bioreactor according to claim 1, characterized in that: A sliding link (96) is installed at the bottom of the barrel (92), and the sliding link (96) is located outside the double guide slide column (10) and is slidably connected. An electromagnetic guide rod (98) is installed at the side end of the sliding link (96), and the side end of the electromagnetic guide rod (98) is inserted through the metering guide (97) and is connected to a push plate (99). A discharge valve (990) is installed at the bottom of the metering guide (97), and the side end surface of the metering guide (97) is connected to an automatic docking guide groove (991). The output end of the variable frequency control motor (91) is connected to a spiral push structure (95).

3. The automatic feeding device for a bioreactor according to claim 1, characterized in that: The side end of the sliding hollow micro pumping seat (154) is fastened to a shell, a connecting frame plate (156) is installed inside the shell, a small driving servo motor (157) is installed at the top and bottom ends of the connecting frame plate (156), and a small rotating gear (158) is connected to the output end of the small driving servo motor (157), and the small rotating gear (158) is arranged in two groups, and there is a distance difference between the two groups of small rotating gears (158).

4. The automatic feeding device for a bioreactor according to claim 3, characterized in that: The bottom ends of one group of small rotating teeth (158) are meshedly connected with inner rotating teeth (159), and the top ends of another group of small rotating teeth (158) are meshedly connected with outer rotating teeth (1590). The side ends of the inner rotating teeth (159) are connected with inner rotating rods (1593), the side ends of the outer rotating teeth (1590) are connected with outer connecting rods (1592), and the side ends of the inner rotating rods (1593) are connected with the first bevel gear (1595).

5. The automatic feeding device for a bioreactor according to claim 4, characterized in that: The bottom end of the first bevel gear (1595) is meshingly connected with the second bevel gear (1596), the top end of the second bevel gear (1596) is connected to the surface of the external connecting rod (1592), the surface of the shell is rotatably connected with the stator and rotor structure (1591), the bottom end of the second bevel gear (1596) is connected to a metering feed conduit (1598), the top of the metering feed conduit (1598) is connected to a microfluidic chip delivery rack end (1597), and the side end of the microfluidic chip delivery rack end (1597) is connected to a sliding hollow micro pumping seat (154) via a flexible telescopic conduit.

6. The automatic feeding device for a bioreactor according to claim 3, characterized in that: The surfaces of the two groups of small rotating teeth (158), the inner rotating teeth (159), the outer rotating teeth (1590), the first bevel gear (1595) and the second bevel gear (1596) are all coated with an antibacterial coating.

7. The automatic feeding device for a bioreactor according to claim 1, characterized in that: The bottom of the reactor body (2) is connected to an exhaust valve pipe (6), the side end of the exhaust valve pipe (6) is connected to a micro worm gear (7), the side end of the micro worm gear (7) and an external piezoelectric ceramic pump are connected to form a closed loop through a line, and the external piezoelectric ceramic pump is used to form a connection with an external multi-channel storage tank.

8. The automatic feeding device for a bioreactor according to claim 1, characterized in that: The top end of the reactor body (2) is connected to a cleaning connecting mist pipe (3), the cleaning connecting mist pipe (3) is connected to an external cleaning mist device, and the bottom side end of the reactor body (2) is connected to a discharge guide valve pipe (8).

9. The automatic feeding device for a bioreactor according to claim 1, characterized in that: A fine-tuning cylinder (13) is installed on the side end of the sliding position seat (11), and a butt feed valve end (14) is slidably connected on the surface of the sliding position seat (11).

10. The automatic feeding device for a bioreactor according to claim 9, characterized in that: The output end of the fine-tuning cylinder (13) is connected to the end (14) of the feed valve, and laser position sensors (12) are installed at the top and bottom of the sliding position seat (11).

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