Wheat gene breeding cultivation device and method

Abstract

The invention discloses a wheat gene breeding cultivation device and method, and relates to the technical field of agricultural planting.The wheat gene breeding cultivation device comprises a heat preservation mechanism, a water inlet pipe, a lifting mechanism and a base, the heat preservation mechanism is pre-buried underground, the water inlet pipe is arranged on one side of the heat preservation mechanism, and one end of the bottom of the water inlet pipe is communicated with the heat preservation mechanism; the other end of the water inlet pipe is connected with an external water source, and sunlight is collected through the light gathering cover and guided into the light guide pipe. The light can be totally reflected at the interface of the inner wall and air, so that a multi-reflection path is formed in the light guide pipe, the sunlight is reflected for multiple times by the light reflecting aluminum foil layer on the inner wall of the light guide pipe, and finally, the sunlight is uniformly scattered to the surface of each layer of culture dish through the tail end lens, so that each layer of wheat seedlings can obtain sufficient and uniform illumination; in the whole process, an external driving mechanism is not needed to adjust the positions of the seedlings, manual intervention is also not needed, use convenience is remarkably improved, and energy consumption is reduced.

Description

Technical Field

[0001] The present invention relates to the field of agricultural planting technology, and in particular to a wheat gene breeding and cultivation device and method. Background Art

[0002] As a cutting-edge biotechnology method, gene breeding technology can quickly breed new varieties with excellent traits such as high yield, high quality, resistance to diseases and pests, and strong stress resistance by precisely regulating the genetic characteristics of wheat; the development of wheat gene breeding and cultivation devices aims to provide an efficient, accurate and controllable experimental platform for gene breeding technology. After searching, the Chinese patent announcement number CN220693945U discloses a wheat seedling cultivation device. Although the device can adjust the position and direction of the seedlings by rotating the mounting bracket so that each plant can obtain sufficient sunlight and air circulation, which is beneficial to the growth and development of the plants, the device requires manual frequent rotation of the mounting bracket during use to ensure that each seedling can obtain sufficient light, which makes the operation more troublesome and reduces the convenience of using the device;

[0003] After searching, the Chinese patent publication number CN218789344U discloses a tea seedling cultivation rack. Although the device can drive the rotating pot to rotate at the same time through the rotating component, so that the cultivation rack can drive the tea seedlings to revolve and rotate at the same time, so that the tea seedlings can receive multi-directional light and the light received is more uniform, but the device needs to drive the seedlings to rotate for a long time through the driving device during use, so that the position of the seedlings can be constantly changed to obtain sufficient light, but the device requires the driving device to be driven for a long time, resulting in high energy consumption. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a wheat genetic breeding and cultivation device and method, which solves the problem that the prior art device mentioned in the background art is troublesome to operate and consumes a lot of energy in long-term use.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a wheat gene breeding and cultivation device and method, comprising a heat preservation mechanism, a water inlet pipe, a lifting mechanism and a base, the heat preservation mechanism is pre-buried underground, a water inlet pipe is arranged on one side of the heat preservation mechanism, one end of the bottom of the water inlet pipe is connected to the heat preservation mechanism, and the other end of the water inlet pipe is connected to an external water source, a top cover is arranged on the top of the heat preservation mechanism, a light guide mechanism is embedded in the top cover, the light guide mechanism is fixed by a support plate, one end of the top of the support plate is glued to the top cover, four groups of support plates are arranged, and each group is provided with two support plates, the four groups of support plates are distributed in annular equidistant shapes on the outside of the layer plate, the outside of the layer plate is welded to the surface of the support plate, the layer plates are distributed vertically equidistantly from top to bottom, a culture dish is placed on the surface of the layer plate, the culture dish is used to cultivate wheat seeds, a lifting mechanism is installed at the bottom of the support plate, the lifting mechanism is used for layer plate lifting and lowering, and the lifting mechanism is installed on the top of the base.

[0006] Preferably, the heat preservation mechanism includes a tank body, the inner wall of the bottom of the tank body is welded to the outer wall of the base, a hollow interlayer is arranged between the outer wall and the inner wall of the tank body, the hollow interlayer inside the tank body is connected with the water inlet pipe, a heating rod is arranged inside the hollow interlayer inside the tank body, the heating rods are distributed in the tank body in a circular shape with equal spacing, an atomizing nozzle is embedded in the inner wall at one end of the top of the tank body, the atomizing nozzle is connected with the interior of the hollow interlayer of the tank body, and the atomizing nozzles are distributed in a circular shape with equal spacing, and by arranging a heating rod in the hollow interlayer of the tank body, the water in the interlayer can be heated; the heated water can evenly transfer heat to the inside of the tank body, thereby realizing temperature control of the cultivation environment; this heating method can ensure uniform distribution of temperature inside the tank body, avoid local temperature being too high or too low, and provide stable temperature conditions for the growth of wheat seedlings.

[0007] Preferably, the light guiding mechanism includes light guiding tubes, and four light guiding tubes are provided. One end of the top of the four light guiding tubes is glued to the top cover, and one end of the top of the light guiding tube is glued with a condenser. One end of the bottom of the light guiding tube is provided with an end lens, and the end lens and the light guiding tube are glued to each other. The end lenses are respectively located at the bottom of the layer plate, and the condenser is located at the top of the light guiding tube. The top surface is a hemispherical structure that can effectively collect natural light and focus it to the entrance of the light guiding tube. The hemispherical condenser can maximize the reception of sunlight from different directions, thereby improving the light collection efficiency. The light is transmitted through multiple reflections in the tube and is finally evenly scattered to the surface of the culture dish at the bottom of the layer plate through the end lens. This design can ensure that the wheat seedlings in each layer of the culture dish can obtain sufficient and uniform light, thereby avoiding growth differences caused by uneven light. The light guiding mechanism uses natural light as the light source, and introduces sunlight into the underground through the design of the condenser and the light guiding tube, without the need for additional lighting equipment or manual intervention; it not only saves energy, but also reduces the complexity of the equipment and the difficulty of operation.

[0008] Preferably, the interior of the light collector is hollow and the top surface of the light collector is hemispherical. The interior of the light guide tube is closed. A reflective aluminum foil layer is provided on the inner wall surface of the light guide tube. The light guide tube is multi-bend. The inner wall of the light guide tube at the bend is smooth. The bottom surface of the end lens is arc-shaped. The top of the light collector is hemispherical. This design can maximize the collection of natural light from different directions. The surface of the hemispherical structure can evenly reflect light to the entrance of the light guide tube, thereby improving the light collection efficiency. The interior of the light collector is hollow, which can reduce the use of materials while ensuring the lightness and light transmittance of the structure, which is conducive to the efficient transmission of light. A reflective aluminum foil layer with high reflectivity is provided on the inner wall surface of the light guide tube. This material can minimize the energy loss of light during transmission and ensure that the light can maintain a high intensity after multiple reflections. The light guide tube is multi-bend. This design can adapt to different spatial layouts so that light can be flexibly transmitted to the required location.

[0009] Preferably, the lifting mechanism includes a driving motor, the driving motor is embedded in the base, a transmission gear is set on the outside of the driving motor output end, the transmission gear is meshed with the driven gear, a fixing block is arranged on the top of the driven gear, the bottom of the fixing block is transmission-connected with the driven gear, a connecting block is arranged on the outside of the fixing block, the connecting block is welded to the fixing block, one end of the bottom of the connecting block is welded to the base, a threaded rod is arranged on the top end of the driven gear, one end of the bottom of the threaded rod is welded to the driven gear, one end of the bottom of the threaded rod is inserted into the inside of the hole groove on the outside of the fixing block, the threaded rod is threadedly connected to the inside of the lifting plate, the top of the lifting plate is welded to the bottom of the support plate, the lifting and lowering of the layer plate is controlled by the driving motor, the operator can conveniently adjust the height of the layer plate, which is convenient for placing and removing the culture dish, this design greatly improves the convenience of operation and reduces the difficulty of manual intervention, when not in use, the layer plate can be retracted to the ground, reducing the space occupancy of the device; not only improves the space utilization rate, but also makes the device more tidy and beautiful when not in use.

[0010] Preferably, the threaded rods are distributed in a circular shape with equal spacing, a limit block is provided at one end of the bottom of the threaded rod, the bottom of the limit block is welded to the top of the threaded rod, the driven gears are distributed in a circular shape with equal spacing on the outside of the transmission gear, and the threaded rods are distributed in a circular shape with equal spacing. This design allows the lifting plate to be subjected to uniform force during the lifting process, and multiple threaded rods share the load at the same time, avoiding tilting or deformation caused by single-point force, thereby improving the stability and reliability of the lifting process, and the threaded rods with equal spacing can ensure the precise movement of the lifting plate in the vertical direction, reduce errors caused by structural deflection, and thus improve the operating accuracy of the entire device.

[0011] Preferably, the top cover is made of transparent tempered glass, the bottom of the top cover is tightly fitted with the top surface of the tank body through lifting and lowering, the top surface of the top cover is flush with the ground, the bottom of the top cover is embedded with a temperature sensor and a humidity sensor, and the temperature sensor and the humidity sensor are connected to the remote terminal signal; the top cover is made of transparent tempered glass, which can efficiently transmit natural light, ensure that sunlight can smoothly enter the inside of the device, and provide sufficient lighting conditions for the wheat seedlings, and the bottom of the top cover is tightly fitted with the top surface of the tank body through lifting and lowering. This design enables the top cover to be lifted and lowered as needed; when it is necessary to operate the internal device (such as placing or removing the culture dish), the top cover can be lifted; during the culture process, the top cover can be lowered and tightly fitted with the tank body to form a sealed space.

[0012] Preferably, the cultivation method comprises the following steps:

[0013] S01: Define breeding goals: Determine specific goals for wheat breeding based on market demand, environmental conditions and grower needs, including but not limited to high yield, disease resistance, stress resistance, high quality or adaptation to specific environments; decompose complex traits (such as high yield) into actionable sub-traits (such as plant height, number of grains per ear, 1000-grain weight), and determine the priority of each trait;

[0014] S02: Parent screening and identification: Screen parent materials with excellent traits from the germplasm resource bank. The parent materials can come from different ecological regions, varieties or wild species; use molecular marker technology to identify the genetic background of the parents to ensure that the parents have excellent genes for the target traits and the genetic background is complementary; the identification method includes but is not limited to analyzing the genome of the parents using single nucleotide polymorphism markers or simple sequence repeat markers; in this step, use a wheat culture device to pre-culture the parent materials, and the culture device can ensure uniform lighting and constant temperature and humidity environmental conditions to ensure the growth consistency of the parent materials in the pre-culture stage;

[0015] S03: Genome analysis and molecular marker development: perform whole genome sequencing on parental materials, analyze their genome structure, and find genes and regulatory elements related to target traits; based on the genome analysis results, develop molecular markers that are closely linked to target traits. Marker types include but are not limited to single nucleotide polymorphism markers, simple sequence repeat markers, or insertion / deletion markers;

[0016] S04: Application of gene editing and transgenic technology: Use gene editing technology to precisely edit the target gene in the parent material to enhance or improve the target trait; editing methods include but are not limited to gene knockout, gene replacement or gene insertion; at the same time, use transgenic technology to introduce exogenous excellent genes (such as disease resistance genes, stress resistance genes or quality improvement genes) into the wheat genome to obtain transgenic wheat materials; transgenic methods include but are not limited to Agrobacterium-mediated transformation or gene gun method;

[0017] S05: Hybridization and molecular marker-assisted selection: Design reasonable hybrid combinations based on the genetic background and target traits of the parents, and obtain hybrid offspring through artificial pollination; among the hybrid offspring, use the developed molecular markers for early screening to quickly identify individuals carrying the target gene and improve selection efficiency; the screening process includes molecular marker-assisted selection of F1 to F3 generations to ensure stable inheritance of the target gene;

[0018] S06: Field trials and trait identification: Plant the progeny selected with molecular markers in the field for multi-generation screening and trait identification; field trials include multi-generation screening of hybrid progeny in different ecological environments to evaluate their performance in actual environments; comprehensive identification of disease resistance, stress resistance, and quality traits of the selected excellent strains to ensure that they meet the breeding goals; identification methods include but are not limited to pathogen inoculation tests, stress tests, and quality analysis;

[0019] S07: Variety approval and promotion and application: Submit excellent strains that have undergone multiple generations of screening and identification to the Variety Approval Committee for variety approval; through seed breeding, technical training and promotion and demonstration, bring new varieties to the market to meet agricultural production needs.

[0020] The present invention provides a wheat gene breeding device and method, which has the following beneficial effects:

[0021] (1) A wheat genetic breeding and cultivation device and method, wherein the device cultivates wheat seedlings in a culture dish, places the culture dish on a multi-layer plate, collects sunlight using a condenser, and guides the sunlight into a light guide; the light will be totally reflected at the interface between the inner wall and the air, thereby forming multiple reflection paths in the light guide; the inner wall of the light guide is covered with a reflective aluminum foil layer with high reflectivity, the surface of which is smooth and the reflectivity is extremely high; when the sunlight enters the light guide, the light will be reflected multiple times on the inner wall; the reflective aluminum foil layer is used to minimize the energy loss of the light during the transmission process, ensuring that the sunlight can be efficiently transmitted to the end of the light guide; the reflective aluminum foil layer on the inner wall of the light guide reflects the sunlight multiple times, and finally the sunlight is evenly scattered to the surface of each layer of the culture dish through the end lens, ensuring that each layer of wheat seedlings can obtain sufficient and uniform light; the entire process does not require an external drive mechanism to adjust the position of the seedlings, and does not require manual intervention, which significantly improves the convenience of use and reduces energy consumption.

[0022] (2) The wheat genetic breeding and cultivation device and method drive the transmission gear to rotate by driving the motor. The transmission gear can drive the driven gear to rotate while rotating. The driven gear can drive the threaded rod to rotate while rotating. The threaded rod can drive the lifting plate to rotate by rotating, so that the layer plate on which the culture dish is placed can be moved up and down. By controlling the lifting of the layer plate, it is convenient to place the culture dish on the surface of the layer plate at a high place, which greatly improves the convenience of using the device. At the same time, the device can drive the layer plate to lift and lower so that multiple layers can be retracted to the ground, which is beneficial to reduce the space occupied. After the layer plate of the device is retracted to the ground, since the condenser is located on the ground, the condenser and the light guide can guide the sunlight to the ground, which can effectively avoid the disadvantage of insufficient lighting underground. In addition, since the soil and underground medium have good thermal barrier properties and large heat capacity, they can effectively buffer the influence of external temperature changes, so that the internal temperature of the device is relatively stable, which is beneficial to the cultivation of small face seedlings.

[0023] (3) In the wheat genetic breeding and cultivation device and method, when the layer plate is retracted to the ground, the interior of the tank body is in a sealed space through the top cover, and the internal temperature is monitored by the temperature sensor and the humidity sensor. When the internal temperature is low, the water in the interlayer inside the tank body is heated by the heating rod. The heated water can evenly transfer heat to the interior of the tank body, which is conducive to achieving a better warming effect. The water in the interlayer of the tank body is atomized and sprayed out by the atomizing nozzle, which can achieve a humidification effect on the interior of the tank body, thereby improving the functionality of the device.

[0024] This solves the problem that the existing device is relatively troublesome to operate and consumes a lot of energy during long-term use. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0026] Figure 2 It is a lifting schematic diagram of the present invention;

[0027] Figure 3 It is a schematic diagram of the internal structure of the present invention;

[0028] Figure 4 This is a schematic diagram of the light guide structure of the present invention;

[0029] Figure 5 It is a schematic diagram of the lifting mechanism structure of the present invention;

[0030] Figure 6 This is a schematic diagram of the structure of the heating rod of the present invention;

[0031] Figure 7 This is a schematic diagram of the support plate structure of the present invention;

[0032] Figure 8 It is a schematic diagram of the top cover structure of the present invention.

[0033] In the figure, 1. insulation mechanism; 101. heating rod; 102. atomizing nozzle; 103. tank body; 2. top cover; 3. light guide mechanism; 301. light guide tube; 302. end lens; 303. condenser; 4. water inlet pipe; 5. support plate; 6. shelf; 7. culture dish; 8. lifting mechanism; 801. transmission gear; 802. driving motor; 803. fixing block; 804. connecting block; 805. driven gear; 806. threaded rod; 807. limit block; 808. lifting plate; 9. base. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Embodiment 1:

[0036] See also Figure 1-8The embodiment of the present invention provides a technical solution: a wheat gene breeding and cultivation device and method, including a heat preservation mechanism 1, a water inlet pipe 4, a lifting mechanism 8 and a base 9, the heat preservation mechanism 1 is pre-buried in the ground, a water inlet pipe 4 is arranged on one side of the heat preservation mechanism 1, one end of the bottom of the water inlet pipe 4 is connected to the heat preservation mechanism 1, and the other end of the water inlet pipe 4 is connected to an external water source, a top cover 2 is arranged on the top of the heat preservation mechanism 1, a light guide mechanism 3 is embedded in the top cover 2, the light guide mechanism 3 is fixed by a support plate 5, one end of the top of the support plate 5 is glued to the top cover 2, four groups of support plates 5 are arranged, and each group is provided with two support plates 5, and the four groups of support plates 5 are distributed in an annular shape with equal spacing on the outside of the layer plate 6, and the outside of the layer plate 6 The support plate 5 is welded to the surface of the support plate 5, and the layer plates 6 are distributed vertically and equidistantly from top to bottom. A culture dish 7 is placed on the surface of the layer plate 6, and the culture dish 7 is used to culture wheat seeds. A lifting mechanism 8 is installed at the bottom of the support plate 5, and the lifting mechanism 8 is used for lifting the layer plate 6. The lifting mechanism 8 is installed on the top of the base 9. The light guide mechanism 3 includes a light guide tube 301, and four light guide tubes 301 are provided. One end of the top of the four light guide tubes 301 is glued to the top cover 2, and a condenser 303 is glued to one end of the top of the light guide tube 301. An end lens 302 is provided at one end of the bottom of the light guide tube 301, and the end lens 302 and the light guide tube 301 are glued to each other. The end lenses 302 are respectively located at the bottom of the layer plate 6, and the condenser 303 is glued to one end of the top of the light guide tube 301. The interior of the light shield 303 is a hollow structure, and the top surface of the condenser 303 is a hemispherical structure. The interior of the light guide 301 is closed, and a reflective aluminum foil layer is provided on the inner wall surface of the light guide 301. The light guide 301 is multi-bend, and the inner wall of the light guide 301 at the bend is a smooth surface. The bottom surface of the end lens 302 is an arc-shaped structure. The device cultivates wheat seedlings in a culture dish 7, places the culture dish 7 on a multi-layer plate 6, collects sunlight using the condenser 303, and guides the sunlight into the light guide 301. The light will be totally reflected at the interface between the inner wall and the air, thereby forming multiple reflection paths in the light guide 301. The inner wall of the light guide 301 is covered with a reflective aluminum foil layer. It is covered with a reflective aluminum foil layer with high reflectivity, and its surface is smooth and has extremely high reflectivity. When sunlight enters the light guide tube 301, the light will be reflected multiple times on the inner wall. The function of the reflective aluminum foil layer is to minimize the energy loss of light during transmission, ensuring that the sunlight can be efficiently transmitted to the end of the light guide tube 301. The reflective aluminum foil layer on the inner wall of the light guide tube 301 reflects the sunlight multiple times, and finally scatters the sunlight evenly to the surface of each layer of culture dish 7 through the end lens 302, ensuring that each layer of wheat seedlings can obtain sufficient and uniform light. The entire process does not require an external driving mechanism to adjust the position of the seedlings, nor does it require manual intervention, which significantly improves the convenience of use and reduces energy consumption.

[0037] Embodiment 2:

[0038] The embodiment of the present invention provides a technical solution: a wheat gene breeding and cultivation device and method, wherein a heat preservation mechanism 1 comprises a tank body 103, wherein the inner wall at the bottom of the tank body 103 is welded to the outer wall of the base 9, a hollow interlayer is arranged between the outer wall and the inner wall of the tank body 103, the hollow interlayer inside the tank body 103 is connected to the water inlet pipe 4, a heating rod 101 is arranged inside the hollow interlayer inside the tank body 103, the heating rod 101 is distributed inside the tank body 103 in an annular shape with equal spacing, an atomizing nozzle 102 is embedded in the inner wall at one end of the top of the tank body 103, the atomizing nozzle 102 is connected to the inside of the hollow interlayer of the tank body 103, and the atomizing nozzle 102 is distributed in an annular shape with equal spacing, the top cover 2 is made of transparent tempered glass, and the bottom of the top cover 2 is lifted and matched with the tank body 103 The top surface fits tightly, and the top surface of the top cover 2 is flush with the ground. A temperature sensor and a humidity sensor are embedded at the bottom of the top cover 2, and the temperature sensor and the humidity sensor are connected to the remote terminal signal. When the layer plate 6 shrinks to the ground, the inside of the tank body 103 is in a sealed space through the top cover 2, and the internal temperature is monitored by the temperature sensor and the humidity sensor. When the internal temperature is low, the water in the interlayer inside the tank body 103 is heated by the heating rod 101, and the heated water can evenly transfer heat to the inside of the tank body 103, which is conducive to achieving a better warming effect. The water inside the interlayer of the tank body 103 is atomized and sprayed out by the atomizing nozzle 102, which can humidify the inside of the tank body 103, thereby improving the functionality of the device.

[0039] Embodiment 3:

[0040] The embodiment of the present invention provides a technical solution: a wheat gene breeding and cultivation device and method, the lifting mechanism 8 includes a driving motor 802, the driving motor 802 is embedded in the base 9, the output end of the driving motor 802 is externally provided with a transmission gear 801, the transmission gear 801 is meshed with a driven gear 805, a fixed block 803 is arranged on the top of the driven gear 805, the bottom of the fixed block 803 is connected to the driven gear 805, a connecting block 804 is arranged on the outside of the fixed block 803, the connecting block 804 is welded to the fixed block 803, one end of the bottom of the connecting block 804 is welded to the base 9, and the top of the driven gear 805 is meshed with the driven gear 805. A threaded rod 806 is provided at one end of the part, and one end of the bottom of the threaded rod 806 is welded to the driven gear 805. One end of the bottom of the threaded rod 806 is inserted into the inner hole groove of the outer side of the fixed block 803, and the threaded rod 806 is connected with the internal thread of the lifting plate 808, and the top of the lifting plate 808 is welded to the bottom of the support plate 5; the threaded rod 806 is distributed in a circular shape with equal spacing, and a limiting block 807 is provided at one end of the bottom of the threaded rod 806. The bottom of the limiting block 807 is welded to the top of the threaded rod 806, and the driven gear 805 is distributed in a circular shape with equal spacing on the outer side of the transmission gear 801, and the transmission gear 801 is driven by the driving motor 802 to rotate When the transmission gear 801 rotates, it can drive the driven gear 805 to rotate. When the driven gear 805 rotates, it can drive the threaded rod 806 to rotate. The threaded rod 806 can drive the lifting plate 808 to rotate by rotating, so that the layer plate 6 on which the culture dish 7 is placed can be moved up and down. By controlling the lifting of the layer plate 6, it is convenient to place the culture dish 7 on the surface of the layer plate 6 at a high place, which greatly improves the convenience of using the device. At the same time, the device can drive the layer plate 6 to rise and fall, so that multiple layer plates 6 can be retracted to the ground, which is conducive to reducing the occupation of space, and the After the device layer plate 6 is retracted underground, since the focus cover 303 is located on the ground, the sunlight can be guided to the underground through the focus cover 303 and the light guide tube 301, which can effectively avoid the disadvantage of insufficient lighting underground. In addition, since the soil and underground medium have good thermal barrier properties and large heat capacity, they can effectively buffer the impact of external temperature changes, making the internal temperature of the device relatively stable, which is conducive to the cultivation of small seedlings. The entire device is controlled by the main control button. Since the equipment matched with the control button is a commonly used equipment and belongs to the existing common sense technology, its electrical connection relationship and specific circuit structure will not be repeated here.

[0041] Embodiment 4:

[0042] The embodiment of the present invention provides a technical solution: a wheat gene breeding device and method, wherein the breeding method steps are as follows:

[0043] S01: Define breeding goals: Determine specific goals for wheat breeding based on market demand, environmental conditions and grower needs, including but not limited to high yield, disease resistance, stress resistance, high quality or adaptation to specific environments; decompose complex traits (such as high yield) into actionable sub-traits (such as plant height, number of grains per ear, 1000-grain weight), and determine the priority of each trait;

[0044] S02: Parent screening and identification: Screen parent materials with excellent traits from the germplasm resource bank. Parent materials can come from different ecological regions, varieties or wild species; use molecular marker technology to identify the genetic background of the parents to ensure that the parents have excellent genes for the target traits and that the genetic backgrounds are complementary; identification methods include but are not limited to analyzing the genome of the parents using single nucleotide polymorphism markers or simple sequence repeat markers; in this step, use a wheat culture device to pre-culture the parent materials. The culture device can ensure uniform lighting and constant temperature and humidity to ensure the growth consistency of the parent materials during the pre-culture stage;

[0045] S03: Genome analysis and molecular marker development: perform whole genome sequencing on parental materials, analyze their genome structure, and find genes and regulatory elements related to target traits; based on the genome analysis results, develop molecular markers that are closely linked to target traits. Marker types include but are not limited to single nucleotide polymorphism markers, simple sequence repeat markers, or insertion / deletion markers;

[0046] S04: Application of gene editing and transgenic technology: Use gene editing technology to precisely edit the target gene in the parent material to enhance or improve the target trait; editing methods include but are not limited to gene knockout, gene replacement or gene insertion; at the same time, use transgenic technology to introduce exogenous excellent genes (such as disease resistance genes, stress resistance genes or quality improvement genes) into the wheat genome to obtain transgenic wheat materials; transgenic methods include but are not limited to Agrobacterium-mediated transformation or gene gun method;

[0047] S05: Hybridization and molecular marker-assisted selection: Design reasonable hybrid combinations based on the genetic background and target traits of the parents, and obtain hybrid offspring through artificial pollination; among the hybrid offspring, use the developed molecular markers for early screening to quickly identify individuals carrying the target gene and improve selection efficiency; the screening process includes molecular marker-assisted selection of F1 to F3 generations to ensure stable inheritance of the target gene;

[0048] S06: Field trials and trait identification: Plant the progeny selected with molecular markers in the field for multi-generation screening and trait identification; field trials include multi-generation screening of hybrid progeny in different ecological environments to evaluate their performance in actual environments; comprehensive identification of disease resistance, stress resistance, and quality traits of the selected excellent strains to ensure that they meet the breeding goals; identification methods include but are not limited to pathogen inoculation tests, stress tests, and quality analysis;

[0049] S07: Variety approval and promotion and application: Submit excellent strains that have undergone multiple generations of screening and identification to the Variety Approval Committee for variety approval; through seed breeding, technical training and promotion and demonstration, bring new varieties to the market to meet agricultural production needs.

[0050] This wheat genetic breeding method combines gene editing technology with molecular marker-assisted selection to achieve precise improvement and rapid screening of wheat target traits; it not only greatly shortens the breeding cycle, but also significantly improves breeding efficiency and accuracy; especially in the screening process of hybrid offspring, the use of molecular marker technology can quickly identify individuals carrying the target gene at the seedling stage, avoiding the screening difficulties and waste of resources caused by the unclear expression of traits in traditional breeding.

[0051] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the attached claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0052] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A wheat gene breeding and cultivation device, characterized in that: The invention comprises a heat preservation mechanism (1), a water inlet pipe (4), a lifting mechanism (8) and a base (9); the heat preservation mechanism (1) is pre-buried underground; a water inlet pipe (4) is arranged on one side of the heat preservation mechanism (1); one end of the bottom of the water inlet pipe (4) is connected to the heat preservation mechanism (1); the other end of the water inlet pipe (4) is connected to an external water source; a top cover (2) is arranged on the top of the heat preservation mechanism (1); a light guide mechanism (3) is embedded in the top cover (2); the light guide mechanism (3) is fixed by a support plate (5); one end of the top of the support plate (5) is glued to the top cover (2); Four groups of support plates (5) are provided, and each group is provided with two support plates (5). The four groups of support plates (5) are distributed in an annular shape at equal intervals on the outside of the layer plate (6). The outside of the layer plate (6) is welded to the surface of the support plate (5). The layer plates (6) are distributed vertically at equal intervals from top to bottom. A culture dish (7) is placed on the surface of the layer plate (6). The culture dish (7) is used to culture wheat seeds. A lifting mechanism (8) is installed at the bottom of the support plate (5). The lifting mechanism (8) is used for lifting the layer plate (6). The lifting mechanism (8) is installed on the top of the base (9).

2. A wheat gene breeding and cultivation device according to claim 1, characterized in that: The heat preservation mechanism (1) comprises a tank body (103), the inner wall of the bottom of the tank body (103) is welded to the outer wall of the base (9), a hollow interlayer is arranged between the outer wall and the inner wall of the tank body (103), the hollow interlayer inside the tank body (103) is connected to the water inlet pipe (4), a heating rod (101) is arranged inside the hollow interlayer inside the tank body (103), the heating rod (101) is distributed in the tank body (103) in an annular shape with equal spacing, an atomizing nozzle (102) is embedded in the inner wall of one end of the top of the tank body (103), the atomizing nozzle (102) is connected to the inside of the hollow interlayer of the tank body (103), and the atomizing nozzle (102) is distributed in an annular shape with equal spacing.

3. A wheat gene breeding and cultivation device according to claim 1, characterized in that: The light guide mechanism (3) comprises a light guide tube (301), four of which are provided, one end of the top of the four light guide tubes (301) being glued to the top cover (2), one end of the top of the light guide tube (301) being glued to a condenser (303), one end of the bottom of the light guide tube (301) being provided with a terminal lens (302), the terminal lens (302) and the light guide tube (301) being glued to each other, and the terminal lenses (302) being respectively located at the bottom of the layer plate (6).

4. A wheat gene breeding and cultivation device according to claim 1, characterized in that: The interior of the condenser (303) is a hollow structure, and the top surface of the condenser (303) is a hemispherical structure. The interior of the light guide (301) is closed. The inner wall surface of the light guide (301) is provided with a reflective aluminum foil layer. The light guide (301) is multi-bend, and the inner wall of the light guide (301) at the bend is a smooth surface. The bottom surface of the terminal lens (302) is an arc-shaped structure.

5. The wheat gene breeding and cultivation device according to claim 1, characterized in that: The lifting mechanism (8) comprises a driving motor (802), the driving motor (802) is embedded in the base (9), a transmission gear (801) is mounted on the outside of the output end of the driving motor (802), the transmission gear (801) is meshed with a driven gear (805), a fixing block (803) is arranged on the top of the driven gear (805), the bottom of the fixing block (803) is transmission-connected to the driven gear (805), a connecting block (804) is arranged on the outside of the fixing block (803), and the connecting block (804) is arranged on the outside of the fixing block (803). The block (804) is welded to the fixed block (803), one end of the bottom of the connecting block (804) is welded to the base (9), one end of the top of the driven gear (805) is provided with a threaded rod (806), one end of the bottom of the threaded rod (806) is welded to the driven gear (805), one end of the bottom of the threaded rod (806) is inserted into the inner hole groove of the outer side of the fixed block (803), the threaded rod (806) is connected to the inner thread of the lifting plate (808), and the top of the lifting plate (808) is welded to the bottom of the support plate (5).

6. A wheat gene breeding and cultivation device according to claim 5, characterized in that: The threaded rods (806) are distributed in a circular shape with equal spacing, a limit block (807) is provided at one end of the bottom of the threaded rod (806), the bottom of the limit block (807) is welded to the top of the threaded rod (806), and the driven gears (805) are distributed in a circular shape with equal spacing on the outside of the transmission gear (801).

7. The wheat gene breeding and cultivation device according to claim 1, characterized in that: The top cover (2) is made of transparent tempered glass. The bottom of the top cover (2) is tightly fitted with the top surface of the tank body (103) through lifting and lowering. The top surface of the top cover (2) is flush with the ground. A temperature sensor and a humidity sensor are embedded in the bottom of the top cover (2), and the temperature sensor and the humidity sensor are connected to the remote terminal signal.

8. A wheat genetic breeding method according to any one of claims 1 to 7, characterized in that: The cultivation method steps are as follows: S01: Define breeding goals: Determine specific goals for wheat breeding based on market demand, environmental conditions and grower needs; S02: Parent screening and identification: Screen parent materials with excellent traits from the germplasm resource bank; use molecular marker technology to identify the genetic background of the parents, use wheat culture equipment to pre-culture the parent materials, ensure uniform lighting and constant temperature and humidity environment conditions, and ensure the growth consistency of the parent materials during the pre-culture stage; S03: Genome analysis and molecular marker development: perform whole genome sequencing on parental materials to analyze their genome structure; Develop molecular markers that are tightly linked to target traits based on genomic analysis results; S04: Application of gene editing and transgenic technology: Use gene editing technology to precisely edit the target genes in parental materials to enhance or improve target traits; S05: Hybridization and molecular marker-assisted selection: Design reasonable hybrid combinations based on the genetic background and target traits of the parents, and obtain hybrid offspring through artificial pollination; S06: Field trials and trait identification: Plant the progeny selected by molecular markers into the field for multi-generation screening and trait identification; S07: Variety approval and promotion and application: Submit excellent strains that have undergone multiple generations of screening and identification to the Variety Approval Committee for variety approval; through seed breeding, technical training and promotion and demonstration, bring new varieties to the market to meet agricultural production needs.

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