A PCR instrument heating device

Abstract

The present invention relates to the technical field of polymerase chain reaction device, in particular to a heating device for a PCR instrument, which includes a fixed seat. A temperature control component is arranged on the fixed seat, a heat conduction module is arranged on the temperature control component, a placement cylinder for placing a PCR tube is arranged on the heat conduction module, and the top surface height of the heat conduction module is flush with the initial solution liquid level in the PCR tube. A first auxiliary heating component for auxiliary heating of the part of the PCR tube above the liquid level is arranged on the heat conduction module, and a second auxiliary heating component for auxiliary heating of the top of the PCR tube is arranged on the fixed seat. This application helps to prevent the generation of condensed water on the inner wall of the PCR tube, thereby helping to make the extractable liquid volume at the bottom of the PCR tube reach the experimental requirement, and further helping to ensure the accuracy of the experimental results of the PCR instrument.

Description

Technical Field

[0001] The present invention relates to the technical field of polymerase chain reaction devices, and more particularly to a heating device for a PCR instrument. Background Art

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. It can be regarded as a special DNA amplification in vitro. Specifically, it consists of several reaction steps such as high-temperature denaturation, low-temperature annealing, and appropriate-temperature extension to form a temperature cycle, enabling the target DNA to be rapidly amplified. It has the characteristics of strong specificity, high sensitivity, simple operation, and time-saving, and is a major innovation in gene amplification technology. The PCR technique can specifically amplify extremely trace targets by millions of times, thus greatly improving the ability to analyze and detect DNA molecules.

[0003] Existing polymerase chain reactions usually use a PCR instrument. A PCR instrument generally includes a housing and a temperature control device. After placing a PCR tube containing reactants inside the temperature control device, it is necessary to heat the temperature inside the PCR tube to 95 degrees Celsius by the temperature control device. The high temperature destroys the hydrogen bonds between the bases on the two strands of the template DNA, causing the two strands to separate and generate single-stranded DNA. At the same time, water vapor is generated during this process. Then, the temperature control device will lower the temperature inside the PCR tube to 50 degrees Celsius to complete low-temperature annealing. At the same time, during this process, the water vapor inside the PCR tube will form condensed water at the tube cap and the tube wall. To improve this defect, a thermal lid is usually added to existing PCR instruments to prevent water vapor from condensing on the tube cap by heating the tube cap.

[0004] However, during the existing use process, condensed water still appears on the inner wall of the PCR tube, and some of the condensed water shows a phenomenon of hanging on the wall, resulting in some water droplets hanging on the inner wall of the PCR tube and not moving downward. As a result, the extractable liquid volume at the bottom of the PCR tube is much lower than the liquid volume required for the experiment, which will further affect the experimental results of the PCR instrument. Summary of the Invention

[0005] In order to prevent condensed water from being generated on the inner wall of the PCR tube, so that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement, and further ensure the accuracy of the experimental results of the PCR instrument, the present application provides a heating device for a PCR instrument.

[0006] The present application provides a heating device for a PCR instrument, adopting the following technical solutions:

[0007] A heating device for a PCR instrument, comprising a fixed seat, a temperature control component is arranged on the fixed seat, a heat conduction module is arranged on the temperature control component, a placement cylinder for placing a PCR tube is arranged on the heat conduction module, and the top surface height of the heat conduction module is flush with the initial solution liquid level in the PCR tube. A first auxiliary heating component for auxiliary heating of the part of the PCR tube above the liquid level is arranged on the heat conduction module, and a second auxiliary heating component for auxiliary heating of the top of the PCR tube is arranged on the fixed seat; a containing cavity for placing the PCR tube is arranged in the placement cylinder. The heat conduction module comprises a heat conduction plate and a heat conduction block. The heat conduction plate is installed on the fixed seat and is placed in contact with the temperature control component. The heat conduction block is arranged on the heat conduction plate and is in contact with the circumferential side wall of the placement cylinder. The heat conduction block comprises a fixed part, a movable part and a connecting component. The fixed part is fixedly arranged on the heat conduction plate. The movable part is arranged above the fixed part and is connected to the fixed part through the connecting component. A height adjusting mechanism for adjusting the top surface height of the movable part is arranged on the heat conduction plate.

[0008] By adopting the above technical solution, setting the height of the heat conduction module helps to improve the concentration of heat received by the solution in the PCR tube, thereby helping to increase the heating rate of the solution in the PCR tube and also helping to reduce the weight of the heat conduction module. The first auxiliary heating component helps to perform auxiliary heating on the part of the PCR tube above the liquid level, thereby helping to maintain the temperature of the part of the PCR tube above the liquid level, and further helping to reduce the condensation probability of the inner wall of the PCR tube in the area above the liquid level, helping to ensure that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement, and helping to ensure the accuracy of the experimental results of the PCR instrument. The second auxiliary heating component helps to perform auxiliary heating on the top of the PCR tube, thereby helping to maintain the temperature of the top of the PCR tube, and further helping to reduce the condensation probability of the inner wall of the top of the PCR tube, further ensuring that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement, and further ensuring the accuracy of the experimental results of the PCR instrument. When the liquid level height of the solution in the PCR tube changes, the height adjusting mechanism helps to adjust the top surface height of the movable part, thereby helping to make the top surface of the heat conduction module flush with the changed solution liquid level, and further helping to improve the applicability of the heat conduction module when heating solutions with different liquid level heights.

[0009] In a specific feasible implementation, the first auxiliary heating component comprises an aluminum substrate and heat insulation cotton. The aluminum substrate is arranged on the heat conduction module, and the aluminum substrate is used to conduct the heat of the heat conduction module to the part of the PCR tube above the liquid level. The heat insulation cotton is arranged on the aluminum substrate, and the heat insulation cotton is used to block the part of the PCR tube above the aluminum substrate from contacting the external cold air.

[0010] By adopting the above technical solution, the aluminum substrate helps to conduct the heat of the heat conduction module to the part of the PCR tube above the liquid level. The heat insulation cotton helps to prevent the part of the PCR tube above the aluminum substrate from contacting the cold air outside, thereby helping to keep warm the part of the PCR tube above the aluminum substrate. The cooperation of the aluminum substrate and the heat insulation cotton helps to assist in heating and keeping warm the part of the PCR tube above the liquid level, thereby helping to maintain the temperature of the part of the PCR tube above the liquid level, and further helping to reduce the condensation probability of the inner wall of the PCR tube in the area above the liquid level, helping to ensure that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement, and helping to ensure the accuracy of the experimental results of the PCR instrument.

[0011] In a specific feasible implementation, the second auxiliary heating component includes a module cover and a heating film. The module cover is arranged on the fixed seat and covers the circumferential outer periphery of the placement cylinder. The heating film is arranged on the inner top wall of the module cover, and the heating film is used to assist in heating the top of the PCR tube through the conduction of the module cover and the placement cylinder.

[0012] By adopting the above technical solution, the heating film helps to conduct heat to the top of the PCR tube through the module cover and the placement cylinder, thereby helping to maintain the temperature of the top of the PCR tube, and further helping to reduce the condensation probability of the inner wall at the top of the PCR, further ensuring that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement, and further ensuring the accuracy of the experimental results of the PCR instrument.

[0013] In a specific feasible implementation, the height adjustment mechanism includes a fixed ring frame. An installation through hole is arranged on the heat conduction block in the vertical direction. The fixed ring frame is arranged on the heat conduction plate and located in the installation through hole. A cylindrical cam is arranged in the fixed ring frame. Two groups of arc-shaped tracks are arranged at intervals on the circumferential side wall of the cylindrical cam, and a vertical track for communicating the two groups of arc-shaped tracks is arranged on the circumferential side wall of the cylindrical cam. A fixed plug is arranged on the inner wall of the fixed ring frame, and the fixed plug is inserted into the arc-shaped track or the vertical track. A rotating column is rotatably arranged on the heat conduction plate, and the cylindrical cam is sleeved on the rotating column in a liftable manner. A plurality of card slots for the fixed plug to be inserted into are further arranged on the circumferential side wall of the cylindrical cam. All the plurality of card slots are communicated with the arc-shaped track and are arranged at intervals above the arc-shaped track. An elastic pressing component for elastically pressing the cylindrical cam is arranged above the cylindrical cam on the rotating column. A linkage component for driving the movable part to move synchronously is arranged on the cylindrical cam.

[0014] By adopting the above technical solution, when adjusting the top surface height of the movable part, the rotating column is used to help drive the cylindrical cam to rotate, so as to help the fixed plug to produce relative displacement in the arc track, and then help the cylindrical cam to move upward while rotating under the joint action of the fixed plug and the arc track, and the linkage assembly is used to help drive the movable part to move upward synchronously while the cylindrical cam moves upward, so as to help adjust the top surface height of the movable part. When the fixed plug moves relatively to the bottom of the slot on the arc track, the elastic tightening assembly is used to help elastically tighten the cylindrical cam, so as to help the fixed plug to be stuck in the slot, fix the position of the cylindrical cam, and then help keep the top surface height of the movable part at the adjusted height. Using a number of slots arranged at intervals helps to adjust the top surface height of the movable part multiple times, so as to help make the top surface height of the movable part flush with the liquid surface of solutions at different heights, and then help to improve the applicability of the thermal conductive module when heating solutions at different liquid levels.

[0015] In a specific possible implementation scheme, the elastic clamping assembly includes a fixing ring and a clamping spring, the fixing ring is sleeved on the rotating column and is located above the cylindrical cam, the clamping spring is sleeved on the rotating column, one end of the clamping spring is connected to the fixing ring, and the other end of the clamping spring is connected to the cylindrical cam.

[0016] By adopting the above technical solution, when the cylindrical cam moves upward while rotating, the use of a clamping spring in conjunction with a fixing ring helps to elastically clamp the cylindrical cam, thereby helping to fit the fixed plug into the slot and fix the position of the cylindrical cam, thereby helping to keep the top surface height of the movable part at the adjusted height.

[0017] In a specific feasible implementation scheme, a movable cavity is provided in the heat conducting plate, a movable seat is provided in the movable cavity which can be raised and lowered, the bottom end of the rotating column is inserted into the movable cavity and is rotatably connected to the movable seat, a telescopic spring is provided on the movable seat, and the end of the telescopic spring away from the movable seat is connected to the inner top wall of the movable cavity.

[0018] By adopting the above technical solution, when the fixed plug is disengaged from the slot, the lifting of the rotating column helps to drive the movable seat to move upward in the movable cavity, so that the telescopic spring is converted into a compressed state, thereby helping to drive the cylindrical cam to move upward, and then helping to make the fixed plug disengage from the slot and enter the arc track. The telescopic spring helps to make the movable seat move downward and reset in the movable cavity under the action of the elastic force of the telescopic spring itself, thereby helping to drive the rotating column and the cylindrical cam to reset.

[0019] In a specific feasible implementation, the linkage assembly includes a connecting ring and a plugging rod. A weight-reducing hole is horizontally penetrated in the movable part. The connecting ring is rotatably connected to the cylindrical cam. The plugging rod is inserted into the weight-reducing hole and one end of the plugging rod is fixedly connected to the connecting ring.

[0020] By adopting the above technical solution, the plugging rod helps to penetrate into the weight-reducing hole, so that the movable part can synchronously lift and lower with the plugging rod. The connecting ring helps to connect the plugging rod to the bottom of the cylindrical cam, so that the displacement of the cylindrical cam in the vertical direction can drive the movable part to synchronously lift and lower, and further helps to adjust the top surface height of the movable part.

[0021] In a specific feasible implementation, the connecting assembly includes a plugging column and a connecting spring. The plugging column is arranged on the fixed part. A plugging hole for the plugging column to insert is arranged on the surface of the movable part facing the fixed part. An installation blind hole is arranged on the surface of the plugging column facing the movable part. One end of the connecting spring is arranged in the installation blind hole, and the other end of the connecting spring is connected to the hole wall of the plugging hole.

[0022] By adopting the above technical solution, the plugging column helps to keep the fixed part and the movable part in contact, so that the heat conduction between the fixed part and the movable part can be realized, and further helps to ensure the heat conduction effect of the movable part on the solution in the PCR tube. The connecting spring helps to realize the elastic connection between the fixed part and the movable part, so that the integrity between the fixed part and the movable part can be improved.

[0023] In a specific feasible implementation, an installation groove is arranged on the circumferential side wall of the cylindrical cam above the arc-shaped track. A communication hole for communicating the installation groove and the clamping groove is arranged on the circumferential side wall of the cylindrical cam. A movable plate is movably arranged in the installation groove. An adjusting bolt is threadedly connected to the cylindrical cam, and the adjusting bolt is rotatably connected to the movable plate. A connecting rod is arranged on the movable plate. The connecting rod passes through the communication hole and is inserted into the clamping groove. An abutting plate is arranged at one end of the connecting rod inserted into the clamping groove.

[0024] By adopting the above technical solution, the adjusting bolt helps to drive the movable plate to lift in the installation groove, so that the abutting plate can be driven to lift and move in the clamping groove through the connecting rod. Further, it helps to adjust the clamping position of the fixed plug in the clamping groove, helps to further finely adjust the top surface height of the movable part, and further improves the applicability of the heat conduction module when heating solutions with different liquid surface heights.

[0025] In summary, the present application includes at least one of the following beneficial technical effects:

[0026] 1. By providing a heat conduction module, a first auxiliary heating component, and a second auxiliary heating component, the height setting of the heat conduction module helps improve the concentration of heat received by the solution in the PCR tube, thereby helping to increase the heating rate of the solution in the PCR tube. At the same time, it also helps reduce the weight of the heat conduction module. The first auxiliary heating component helps to auxiliary heat the part of the PCR tube above the liquid level, thereby helping to maintain the temperature of the part of the PCR tube above the liquid level, and further helping to reduce the condensation probability on the inner wall of the part of the PCR tube above the liquid level. The second auxiliary heating component helps to auxiliary heat the top of the PCR tube, thereby helping to maintain the temperature of the top of the PCR tube, and further helping to reduce the condensation probability on the inner wall of the top of the PCR tube. The cooperation of the first auxiliary heating component and the second auxiliary heating component helps ensure that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement, thereby helping to ensure the accuracy of the experimental results of the PCR instrument.

[0027] 2. By providing a height adjustment mechanism, the height adjustment mechanism helps to adjust the top surface height of the movable part, thereby helping to make the top surface of the heat conduction module flush with the changed solution liquid level, and further helping to improve the applicability of the heat conduction module when heating solutions with different liquid levels. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is the overall structural schematic diagram of an embodiment of the present application.

[0029] Figure 2 is the overall structural sectional view of an embodiment of the present application.

[0030] Figure 3 is Figure 2 the enlarged view of part A in

[0031] Figure 4 is the schematic diagram showing the height relationship between the solution liquid level in the PCR tube and the top surface of the heat conduction block.

[0032] Figure 5 is the schematic diagram showing the specific structure of the heat conduction block and the connection component.

[0033] Figure 6 is Figure 2 the enlarged view of part B in

[0034] Figure 7 is the schematic diagram showing the connection relationship between the fixed ring frame and the cylindrical cam.

[0035] Figure 8 is the sectional view showing the specific mechanism on the cylindrical cam.

[0036] Figure 9 is the schematic diagram showing the specific structure of the linkage component.

[0037] Description of reference numerals: 1, fixing seat; 2, temperature control unit; 3, placement column; 31, accommodating cavity; 4, first auxiliary heating component; 41, aluminum substrate; 42, thermal insulation cotton; 5, second auxiliary heating component; 51, module cover; 52, heating film; 6, heat conducting plate; 7, heat conducting block; 8, fixing part; 9, movable part; 10, connecting component; 101, plug-in column; 102, connecting spring; 11, plug-in hole; 12, installation blind hole; 13, fixing ring frame; 14, installation through hole; 15 , cylindrical cam; 16, arc track; 17, vertical track; 18, fixed plug; 19, rotating column; 20, slot; 21, elastic clamping assembly; 211, fixed ring; 212, clamping spring; 22, linkage assembly; 221, connecting ring; 222, plug-in rod; 23, movable cavity; 24, movable seat; 25, telescopic spring; 26, weight-reducing hole; 27, mounting slot; 28, connecting hole; 29, movable plate; 30, adjusting bolt; 32, connecting rod; 33, abutment plate. DETAILED DESCRIPTION

[0038] The present application is further described in detail below in conjunction with the accompanying drawings.

[0039] The present application discloses a PCR instrument heating device, referring to Figure 1 and Figure 2 , including a fixing seat 1, on which a plurality of temperature control components 2 are fixedly mounted. In this embodiment, the temperature control components 2 are semiconductor cooling sheets. A heat conduction module is placed on the plurality of semiconductor cooling sheets. The heat conduction module includes a heat conduction plate 6 and a heat conduction block 7. The heat conduction plate 6 is placed on the top surface of the plurality of semiconductor cooling sheets in a fit, and the heat conduction plate 6 is fixedly connected to the fixing seat 1 by screws. The heat conduction block 7 is placed on the top surface of the heat conduction plate 6. A plurality of placement columns 3 are fixedly mounted on the top surface of the heat conduction plate 6 at intervals, and each placement column 3 is provided with a receiving cavity 31, and the circumferential side wall of each placement column 3 is fitted with the heat conduction block 7.

[0040] Reference Figure 2 and Figure 3 A first auxiliary heating component 4 is arranged on the heat conducting module, and the first auxiliary heating component 4 includes an aluminum substrate 41 and a thermal insulation cotton 42. The aluminum substrate 41 is fixedly placed on the top surface of the heat conducting block 7, and the aluminum substrate 41 is fitted and sleeved on the circumferential periphery of multiple placement columns 3. The thermal insulation cotton 42 is fixedly placed on the top surface of the aluminum substrate 41, and the thermal insulation cotton 42 is fitted and sleeved on the circumferential periphery of multiple placement columns 3.

[0041] Reference Figure 2 and Figure 3, a second auxiliary heating component 5 is provided on the fixing base 1. The second auxiliary heating component 5 includes a module cover 51 and a heating film 52. The module cover 51 is fixedly covered on the top of the fixing base 1. The top wall of the module cover 51 is located above the heat-insulating cotton 42. The top wall of the module cover 51 is fitted and sleeved on the outer periphery of the tops of a plurality of placing cylinders 3, and the top surface of the module cover 51 is flush with the top surface of the placing cylinders 3. The heating film 52 is fixedly installed on the inner top wall of the module cover 51.

[0042] Referring to Figure 1 and Figure 4 , when performing a PCR reaction, a PCR tube containing 30 μL of solution is snap-fitted and placed into the accommodation cavity 31, so that the height of the initial solution level in the PCR tube is flush with the top surface of the heat conduction block 7.

[0043] Referring to Figure 2 and Figure 3 , start a plurality of semiconductor refrigeration chips, so that the temperatures of the plurality of semiconductor refrigeration chips rise to 95 degrees Celsius. At the same time, conduct the heat on the semiconductor refrigeration chips to the inside of the PCR tube through the sequential conduction of the heat conduction plate 6, the heat conduction block 7 and the placing cylinders 3, and heat the solution in the PCR tube to 95 degrees Celsius; then, cooperate with the heat conduction plate 6, the heat conduction block 7 and the placing cylinders 3 through the semiconductor refrigeration chips to make the temperature of the solution in the PCR tube drop to 50 degrees Celsius.

[0044] Referring to Figure 3 and Figure 4 , during the process of raising and lowering the temperature of the solution in the PCR tube, the heat on the heat conduction block 7 will be conducted to the aluminum substrate 41 at the same time. The aluminum substrate 41 then conducts the heat to the part of the PCR tube above the liquid level, and performs auxiliary heating on the part of the PCR tube near the upper part of the liquid level. At the same time, the heat-insulating cotton 42 blocks the part of the PCR tube above the aluminum substrate 41 from contacting the outside cold air, which helps to maintain the temperature of the part of the PCR tube above the liquid level, and further helps to reduce the condensation probability of the inner wall of the part of the PCR tube above the liquid level, helps to ensure that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement, and thus helps to ensure the accuracy of the experimental results of the PCR instrument.

[0045] Referring to Figure 3 and Figure 4 , during the process of raising and lowering the temperature of the solution in the PCR tube, continuously heat the module cover 51 through the heating film 52, so that the temperature of the top wall of the module cover 51 is maintained at 60 degrees Celsius, and conduct the heat to the top of the PCR tube through the module cover 51, which helps to maintain the temperature of the top of the PCR tube, and further helps to reduce the condensation probability of the inner wall at the top of the PCR, further ensures that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement, and further ensures the accuracy of the experimental results of the PCR instrument.

[0046] Referring toFigure 5 , the heat conducting block 7 includes a fixed part 8 and a movable part 9. The fixed part 8 is fixedly installed on the top surface of the heat conducting plate 6. The movable part 9 is placed above the fixed part 8, and a plurality of groups of connecting components 10 are arranged between the fixed part 8 and the movable part 9. Each group of connecting components 10 includes a plugging column 101 and a connecting spring 102. The plugging column 101 is fixedly installed on the top surface of the fixed part 8. A plugging hole 11 is formed on the bottom surface of the movable part 9, and the plugging column 101 is plugged in the plugging hole 11. An installation blind hole 12 is axially formed on the top surface of the plugging column 101. One end of the connecting spring 102 is fixedly connected to the bottom hole wall of the installation blind hole 12, and the other end of the connecting spring 102 is fixedly connected to the top hole wall of the plugging hole 11.

[0047] Refer to Figure 2 and Figure 6 , a height adjusting mechanism acting on the movable part 9 is arranged on the heat conducting plate 6. The height adjusting mechanism includes a fixed ring frame 13. An installation through hole 14 is jointly formed in the central positions of the fixed part 8 and the movable part 9 in the vertical direction. The fixed ring frame 13 is fixedly installed on the heat conducting plate 6 and is located inside the installation through hole 14.

[0048] Refer to Figure 6 and Figure 7 , a cylindrical cam 15 is movably placed inside the fixed ring frame 13. The cylindrical cam 15 is coaxially arranged with the fixed ring frame 13, and the circumferential side wall of the cylindrical cam 15 is in sliding fit with the circumferential inner wall of the fixed ring frame 13. Two groups of arc-shaped tracks 16 are spaced apart on the circumferential side wall of the cylindrical cam 15, and two vertical tracks 17 are also spaced apart on the circumferential side wall of the cylindrical cam 15. The top end of the arc-shaped track 16 is communicated with the top end of one vertical track 17, and the bottom end is communicated with the bottom end of the other vertical track 17. A fixed plug 18 is fixedly installed on the top inner wall of the fixed ring frame 13. In the initial state, the fixed plug 18 is plugged in the top end of one vertical track 17.

[0049] Refer to Figure 6 , an activity cavity 23 is formed inside the heat conducting plate 6 below the cylindrical cam 15. An activity seat 24 can be placed in the activity cavity 23 in a lifting manner. A rotating column 19 is rotatably installed on the top surface of the activity seat 24. A telescopic spring 25 is sleeved on a section of the rotating column 19 located inside the activity cavity 23. One end of the telescopic spring 25 is fixedly connected to the activity seat 24, and the other end is fixedly connected to the inner top wall of the activity cavity 23. The rotating column 19 is coaxially arranged with the cylindrical cam 15 and penetrates through the cylindrical cam 15. The cylindrical cam 15 and the rotating column 19 are connected in a liftable and clamped manner.

[0050] Refer to Figure 8, on the circumferential side wall of the cylindrical cam 15 above each arc-shaped track 16, a plurality of card slots 20 are provided, and the plurality of card slots 20 are arranged at intervals and are all communicated with the arc-shaped track 16. On the circumferential side wall of the cylindrical cam 15 above each arc-shaped track 16, an installation groove 27 is also provided. The installation groove 27 is located above the plurality of card slots 20. A plurality of communication holes 28 are provided on the circumferential side wall of the cylindrical cam 15. One end of the communication hole 28 is communicated with the card slot 20, and the other end is communicated with the installation groove 27. A movable plate 29 is liftably clamped in the installation groove 27. The top of the cylindrical cam 15 is threadedly connected with an adjusting bolt 30, and the bottom end of the adjusting bolt 30 is rotatably connected with the movable plate 29. A plurality of connecting rods 32 are fixedly connected to the movable plate 29. The connecting rods 32 pass through the communication holes 28 and are inserted into the card slots 20. One end of the connecting rod 32 inserted into the card slot 20 is fixedly connected with an abutting plate 33.

[0051] Referring to Figure 6 and Figure 8 , an elastic abutting assembly 21 is provided on the rotating column 19. The elastic abutting assembly 21 includes a fixed ring 211 and an abutting spring 212. The fixed ring 211 is fixedly sleeved on the circumference of the rotating column 19 and is located above the cylindrical cam 15. The abutting spring 212 is sleeved on the rotating column 19, and one end of the abutting spring 212 is fixedly connected with the fixed ring 211, and the other end is fixedly connected with the cylindrical cam 15.

[0052] Referring to Figure 6 and Figure 9 , a linkage assembly 22 is provided at the bottom of the cylindrical cam 15. The linkage assembly 22 includes a connecting ring 221 and a plurality of insertion rods 222. A weight-reducing hole 26 is horizontally penetrated in the movable part 9. The connecting ring 221 is rotatably connected to the bottom surface of the cylindrical cam 15. The plurality of insertion rods 222 are inserted into the weight-reducing hole 26, and one ends of the plurality of insertion rods 222 are fixedly connected to the circumferential side wall of the connecting ring 221.

[0053] Referring to Figure 6 and Figure 7 , when performing PCR reactions on solutions of different volumes, that is, when the liquid level height of the solution in the PCR tube changes, the operator rotates the rotating column 19 to drive the cylindrical cam 15 to rotate synchronously, so that the fixed plug 18 generates a relative displacement in the arc-shaped track 16, and the cylindrical cam 15 moves upward under the combined action of the fixed plug 18 and the arc-shaped track 16, that is, the cylindrical cam 15 moves upward while rotating. When the cylindrical cam 15 moves upward, the plurality of insertion rods 222 are driven to move upward synchronously through the connecting ring 221, thereby driving the movable part 9 to move upward, and further adjusting the top surface height of the movable part 9, which helps to make the top surface of the movable part 9 flush with the changed liquid level of the solution, and thus helps to improve the applicability of the heat conduction module when heating solutions with different liquid level heights.

[0054] Referring to Figure 5 , during the upward movement of the movable part 9, the insertion post 101 slides relative to the insertion hole 11, and the insertion post 101 always remains in contact with the hole wall of the insertion hole 11, which helps to achieve heat conduction between the fixed part 8 and the movable part 9, and further helps to ensure the heat conduction effect of the movable part 9 on the solution in the PCR tube.

[0055] Referring to Figure 7 and Figure 8 , when the relative position of the fixed plug 18 in the arc-shaped track 16 is below the card slot 20, due to the elastic tightening of the cylindrical cam 15 by the tightening spring 212 in cooperation with the fixed ring 211, the cylindrical cam 15 has a tendency to move downward, and further the fixed plug 18 is snapped into the inside of the card slot 20 and abuts against the abutting plate 33, which helps to position the cylindrical cam 15 through the fixed plug 18, and thus helps to keep the top surface height of the movable part 9 stable after adjustment.

[0056] Referring to Figure 6 and Figure 7 , when the top surface height of the movable part 9 needs to be adjusted again, the operator lifts the rotating column 19 to drive the movable seat 24 to move upward in the movable cavity 23, so that the telescopic spring 25 is converted into a compressed state, and at the same time drives the cylindrical cam 15 to move upward, so that the fixed plug 18 is disengaged from the inside of the card slot 20 and enters the arc-shaped track 16.

[0057] Referring to Figure 7 and Figure 8 , the operator rotates the rotating column 19 to deviate the fixed plug 18 from the opening of the card slot 20, and then releases the lifting of the rotating column 19. Due to the elastic force of the telescopic spring 25 itself, the movable seat 24 moves downward and resets in the movable cavity 23, thereby driving the rotating column 19 and the cylindrical cam 15 to reset. Then the operator rotates the rotating column 19 again, so that the fixed plug 18 is snapped into another card slot 20, and the top surface height of the movable part 9 can be adjusted again.

[0058] Referring to Figure 7 and Figure 8 , when the fixed plug 18 relatively moves to the intersection position of the arc-shaped track 16 and the vertical track 17 at the bottom, due to the elastic tightening of the cylindrical cam 15 by the tightening spring 212 in cooperation with the fixed ring 211, the cylindrical cam 15 has a tendency to move downward, and further the fixed plug 18 relatively moves to the top of the vertical track 17 in the vertical track 17, that is, the cylindrical cam 15 moves downward to the initial position.

[0059] Referring to Figure 7 and Figure 8, when it is necessary to finely adjust the top surface height of the movable part 9, the operator rotates the adjusting bolt 30 to drive the movable plate 29 to move up and down in the installation groove 27, so as to drive the abutting plate 33 to move up and down inside the clamping groove 20 through the connecting rod 32, and then finely adjust the clamping position of the fixed plug 18 inside the clamping groove 20, which helps to further finely adjust the top surface height of the movable part 9, and further improves the applicability of the heat conduction module when heating solutions with different liquid surface heights.

[0060] The implementation principle of the embodiment of this application is as follows: When performing a PCR reaction, a PCR tube containing 30 μL of solution is clamped and placed in the accommodation cavity 31, so that the initial liquid surface height in the PCR tube is flush with the top surface of the heat conduction block 7. Start multiple semiconductor refrigeration chips to raise the temperature of the multiple semiconductor refrigeration chips to 95 degrees Celsius, and at the same time conduct the heat on the semiconductor refrigeration chips to the inside of the PCR tube through the sequential conduction of the heat conduction plate 6, the heat conduction block 7 and the placement cylinder 3, and heat the solution in the PCR tube to 95 degrees Celsius; then, cooperate with the semiconductor refrigeration chips, the heat conduction plate 6, the heat conduction block 7 and the placement cylinder 3 to lower the temperature of the solution in the PCR tube to 50 degrees Celsius.

[0061] During the process of raising and lowering the temperature of the solution in the PCR tube, the heat on the heat conduction block 7 will be conducted to the aluminum substrate 41 at the same time, and the aluminum substrate 41 will then conduct the heat to the part of the PCR tube above the liquid surface, assisting in heating the part of the PCR tube near the liquid surface, and at the same time, the heat insulation cotton 42 blocks the part of the PCR tube above the aluminum substrate 41 from contacting the outside cold air, which helps to maintain the temperature of the part of the PCR tube above the liquid surface, and further helps to reduce the condensation probability of the inner wall of the PCR tube in the area above the liquid surface, helps to ensure that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement, and thus helps to ensure the accuracy of the experimental results of the PCR instrument.

[0062] During the process of raising and lowering the temperature of the solution in the PCR tube, the module cover 51 is continuously heated by the heating film 52, so that the top wall temperature of the module cover 51 is maintained at 60 degrees Celsius, and the heat is conducted to the top of the PCR tube through the module cover 51, which helps to maintain the temperature of the top of the PCR tube, and further helps to reduce the condensation probability of the inner wall of the top of the PCR tube, further ensuring that the extractable liquid volume at the bottom of the PCR tube reaches the experimental requirement and further ensuring the accuracy of the experimental results of the PCR instrument.

[0063] When performing PCR reactions on solutions of different volumes, that is, when the liquid level height of the solution in the PCR tube changes, the operator rotates the rotating column 19 to drive the cylindrical cam 15 to rotate synchronously, causing the fixed plug 18 to generate a relative displacement within the arc-shaped track 16, such that the cylindrical cam 15 moves upward under the combined action of the fixed plug 18 and the arc-shaped track 16, that is, the cylindrical cam 15 moves upward while rotating. When the cylindrical cam 15 moves upward, it drives multiple insertion rods 222 to move upward synchronously through the connecting ring 221, thereby driving the movable part 9 to move upward, and further adjusting the top surface height of the movable part 9, which helps to make the top surface of the movable part 9 flush with the changed liquid level of the solution, thus helping to improve the applicability of the heat conduction module when heating solutions with different liquid level heights.

[0064] During the upward movement of the movable part 9, the insertion column 101 slides relatively within the insertion hole 11, and the insertion column 101 always remains in contact with the hole wall of the insertion hole 11, which helps to achieve heat conduction between the fixed part 8 and the movable part 9, and further helps to ensure the heat conduction effect of the movable part 9 on the solution in the PCR tube.

[0065] When the relative position of the fixed plug 18 within the arc-shaped track 16 is below the card slot 20, since the pressing spring 212 elastically presses the cylindrical cam 15 in cooperation with the fixed ring 211, the cylindrical cam 15 has a tendency to move downward, and thus the fixed plug 18 is inserted into the interior of the card slot 20 and abuts against the abutting plate 33, which helps to position the cylindrical cam 15 through the fixed plug 18, and thus helps to keep the adjusted top surface height of the movable part 9 stable.

[0066] The above are all preferred embodiments of this application, and the protection scope of this application is not limited thereby. Therefore, all equivalent changes made according to the structure, shape, and principle of this application should be covered within the protection scope of this application.

Claims

1. A heating device for a PCR instrument, characterized in that: It includes a fixed base (1), a temperature control component (2) is arranged on the fixed base (1), a heat conduction module is arranged on the temperature control component (2), a placement cylinder (3) for placing a PCR tube is arranged on the heat conduction module, and the top surface height of the heat conduction module is flush with the initial liquid level in the PCR tube. A first auxiliary heating component (4) for assisting in heating the part of the PCR tube above the liquid level is arranged on the heat conduction module, and a second auxiliary heating component (5) for assisting in heating the top of the PCR tube is arranged on the fixed base (1); a receiving cavity (31) for placing a PCR tube is arranged in the placement cylinder (3). The heat conduction module includes a heat conduction plate (6) and a heat conduction block (7). The heat conduction plate (6) is installed on the fixed base (1) and is placed in contact with the temperature control component (2). The heat conduction block (7) is arranged on the heat conduction plate (6) and is in contact with the circumferential side wall of the placement cylinder (3). The heat conduction block (7) includes a fixed part (8), a movable part (9) and a connecting component (10). The fixed part (8) is fixedly arranged on the heat conduction plate (6). The movable part (9) is arranged above the fixed part (8) and is connected to the fixed part (8) through the connecting component (10). A height adjustment mechanism for adjusting the top surface height of the movable part (9) is arranged on the heat conduction plate (6). The first auxiliary heating component (4) includes an aluminum substrate (41) and heat insulation cotton (42). The aluminum substrate (41) is arranged on the heat conduction module, and the aluminum substrate (41) is used to conduct the heat of the heat conduction module to the part of the PCR tube above the liquid level. The heat insulation cotton (42) is arranged on the aluminum substrate (41), and the heat insulation cotton (42) is used to prevent the part of the PCR tube above the aluminum substrate (41) from contacting the external cold air. The second auxiliary heating component (5) includes a module cover (51) and a heating film (52). The module cover (51) is arranged on the fixed base (1) and covers the circumferential periphery of the placement cylinder (3). The heating film (52) is arranged on the inner top wall of the module cover (51), and the heating film (52) is used to assist in heating the top of the PCR tube through the conduction of the module cover (51) and the placement cylinder (3).

2. The heating device of a PCR instrument according to claim 1, characterized in that: The height adjustment mechanism includes a fixed ring frame (13). An installation through hole (14) is arranged on the heat conduction block (7) in the vertical direction. The fixed ring frame (13) is arranged on the heat conduction plate (6) and located inside the installation through hole (14). A cylindrical cam (15) is arranged inside the fixed ring frame (13). Two groups of arc-shaped tracks (16) are arranged at intervals on the circumferential side wall of the cylindrical cam (15), and a vertical track (17) for communicating the two groups of arc-shaped tracks (16) is arranged on the circumferential side wall of the cylindrical cam (15). A fixed plug (18) is arranged on the inner wall of the fixed ring frame (13), and the fixed plug (18) is inserted into the arc-shaped track (16) or the vertical track (17). A rotating column (19) is rotatably arranged on the heat conduction plate (6), and the cylindrical cam (15) is sleeved on the rotating column (19) in a liftable manner. A plurality of card slots (20) for the fixed plug (18) to be inserted into are also arranged on the circumferential side wall of the cylindrical cam (15). All the plurality of card slots (20) are communicated with the arc-shaped track (16) and are arranged at intervals above the arc-shaped track (16). An elastic pressing component (21) for elastically pressing the cylindrical cam (15) is arranged above the cylindrical cam (15) on the rotating column (19). A linkage component (22) for driving the movable part (9) to move synchronously is arranged on the cylindrical cam (15).

3. The heating device of a PCR instrument according to claim 2, wherein: The elastic pressing component (21) includes a fixed ring (211) and a pressing spring (212). The fixed ring (211) is sleeved on the rotating column (19) and located above the cylindrical cam (15). The pressing spring (212) is sleeved on the rotating column (19), and one end of the pressing spring (212) is connected to the fixed ring (211), and the other end of the pressing spring (212) is connected to the cylindrical cam (15).

4. The heating device of a PCR instrument according to claim 2, characterized in that: An activity cavity (23) is arranged inside the heat conduction plate (6). An activity seat (24) is arranged in the activity cavity (23) in a liftable manner. The bottom end of the rotating column (19) is inserted into the activity cavity (23) and is rotatably connected to the activity seat (24). A telescopic spring (25) is arranged on the activity seat (24), and the end of the telescopic spring (25) far away from the activity seat (24) is connected to the inner top wall of the activity cavity (23).

5. The heating device of a PCR instrument according to claim 2, wherein: The linkage component (22) includes a connecting ring (221) and a plugging rod (222). A weight-reducing hole (26) is arranged horizontally through the movable part (9). The connecting ring (221) is rotatably connected to the cylindrical cam (15). The plugging rod (222) is inserted into the weight-reducing hole (26), and one end of the plugging rod (222) is fixedly connected to the connecting ring (221).

6. The heating device of a PCR instrument according to claim 1, characterized in that: The connection component (10) includes a plug post (101) and a connection spring (102). The plug post (101) is arranged on the fixed part (8). A plug hole (11) for the plug post (101) to insert is arranged on the surface of the movable part (9) facing the fixed part (8). An installation blind hole (12) is arranged on the surface of the plug post (101) facing the movable part (9). One end of the connection spring (102) is arranged in the installation blind hole (12), and the other end of the connection spring (102) is connected to the hole wall of the plug hole (11).

7. The heating device of a PCR instrument according to claim 2, wherein: An installation groove (27) is arranged on the circumferential side wall of the cylindrical cam (15) above the arc-shaped track (16). A communication hole (28) for communicating the installation groove (27) and the clamping groove (20) is arranged on the circumferential side wall of the cylindrical cam (15). A movable plate (29) is movably arranged in the installation groove (27). An adjusting bolt (30) is threadedly connected to the cylindrical cam (15), and the adjusting bolt (30) is rotatably connected to the movable plate (29). A connecting rod (32) is arranged on the movable plate (29). The connecting rod (32) passes through the communication hole (28) and is inserted into the clamping groove (20). A contact plate (33) is arranged at one end of the connecting rod (32) inserted into the clamping groove (20).

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