Sampling device for immune infiltration

Abstract

The invention discloses a sampling device for immune infiltration, and relates to the technical field of tumor sampling, the sampling device comprises a cutting sampling assembly, and the cutting sampling assembly comprises a hollow cam, a side groove, a sampling port, an end groove, an end sliding pin and a cutting knife. In the using process, through the structural design that the side groove and the end groove are correspondingly formed in the circumferential direction and the end of the hollow cam, linear motion of the lower portal frame driven by the needle rod can be converted into rotary motion of the hollow cam, and synchronous opening and closing of the cutting knives on the two sides of the flange are further controlled through the rotary motion of the hollow cam; the inner cavity of the hollow cam can be filled with required medical liquid medicine in advance through the hollow design of the middle of the hollow cam, the lower piston is driven by the needle rod to be pumped in the cavity of the hollow cam, and the medical liquid medicine is sprayed to the focus part of a patient to meet the functional requirement; and the applicability is high.

Description

Technical Field

[0001] The present invention relates to the technical field of tumor sampling, and specifically provides a sampling device for immune infiltration. Background Art

[0002] Immune infiltration generally refers to the aggregation of immune cells in tumor or diseased tissues. Studying these cells helps to understand the immune microenvironment of the disease. Therefore, the sampling device needs to be able to effectively collect samples from these tissues, especially the part containing immune cells.

[0003] For example, the invention with the publication number CN107928714A discloses a tumor sampling device. This invention proposes to use negative pressure for adsorption to achieve the effect of rapid sampling to prevent patient pain. However, in the actual use process, since the tumor mass as a whole is a large tissue mass, the collection efficiency of directly adsorbing it is low, and it is difficult to remove a relatively complete tumor mass that can be used for observation from the tumor mass, having the disadvantage of poor sampling effect, and a negative pressure system needs to be connected during use, resulting in a high use cost.

[0004] Therefore, in view of this, in view of the deficiencies of the existing structure, research and improvement are carried out, and a sampling device for immune infiltration is proposed. Summary of the Invention

[0005] The purpose of the present invention is to provide a sampling device for immune infiltration to solve the problems raised in the above background art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A sampling device for immune infiltration, including a cutting and sampling assembly. The cutting and sampling assembly includes a hollow cam, a side groove, a sampling port, an end groove, an end sliding pin, and a cutting knife. The hollow cam is provided with a cavity along the axial direction inside, and a side groove is opened on the side surface of the hollow cam. A sampling port is opened in the middle of the end surface of the hollow cam, and end grooves are opened on both sides of the end surface of the hollow cam. End sliding pins are slidably installed in the corresponding end grooves on both sides, and the end sliding pins are integrally fixed to the end planes of the cutting knives on both sides.

[0007] Further, the side groove opened on the side surface of the hollow cam is a "W"-shaped closed ring structure, and the end groove opened on the end surface of the hollow cam is a two-stage involute structure.

[0008] Further, the hollow cam is rotatably installed at the inner bottom end of the sampler, and the central axis of the inner cavity of the sampler coincides with the axial direction of the hollow cam axis.

[0009] Further, a ring-shaped flange is integrally fixed to the outer edge of the end of the sampler, and through holes for sliding cooperation with the corresponding cutting knives are provided on both sides of the flange.

[0010] Further, a limiting ring is fixedly installed in the middle of the sampler, and side holes are symmetrically opened on both sides of the limiting ring.

[0011] Further, a gantry is slidably installed inside the side holes on both sides, and side sliding pins are arranged on the inner sides of the ends of the gantry, which are matched with the side grooves opened on the side surfaces of the hollow cam. Moreover, a first spring elastically matched with the gantry is arranged above the side holes.

[0012] Further, the side sliding pins on the inner sides of the ends of the gantry drive the hollow cam to rotate by themselves through the cooperation with the side grooves. During the rotation of the hollow cam, the end grooves on both sides of the end surface drive the two cutting knives on both sides to open and close synchronously through the cooperation with the corresponding end sliding pins.

[0013] Further, a middle hole is penetrated through the middle of the limiting ring, and a needle rod is slidably installed inside the middle hole.

[0014] Further, a piston is connected to the bottom end of the needle rod, and the piston is tightly fitted with the inner wall of the cavity of the hollow cam through a sealing ring. Moreover, a needle handle is fixedly connected to the top end of the needle rod.

[0015] Further, a middle ring is fixedly installed in the middle of the needle rod, and the middle ring is elastically matched with the end plane of the gantry through a second spring. Moreover, the elastic coefficient of the second spring is greater than that of the first spring.

[0016] The present invention provides a sampling device for immune infiltration, which has the following beneficial effects;

[0017] 1. During the use of the present invention, in this application, not only through the structural design of providing side grooves and end grooves corresponding to the circumferential direction and the end of the hollow cam, the linear motion of the gantry driven by the needle rod can be converted into the rotational motion of the hollow cam, and further through the rotational motion of the hollow cam, the synchronous opening and closing of the cutting knives on both sides of the flange can be controlled, so as to realize the opening and closing control of the sampling port at the end of the sampler. Moreover, through the hollow design in the middle of the hollow cam, the internal cavity of the hollow cam can be pre-filled with the required medical liquid. Through the pumping of the piston located in the cavity of the hollow cam driven by the needle rod, the medical liquid is sprayed on the lesion site of the patient to meet the functional requirements. While the structure is compact, the linkage and cooperation between components are strong.

[0018] 2. During the use of the present invention, on the one hand, the generation of negative pressure does not rely on an external negative pressure pipeline system, but relies on the generation of negative pressure during the process of the piston resetting inside the cavity of the hollow cam to realize the negative pressure adsorption of the tumor mass. And on the other hand, through the self-rotation and reset of the hollow cam to drive the closing and cutting of the cutting knives on both sides of the flange, a relatively complete tumor mass that can be used for observation can be taken from the tumor mass. The sampling is complete to avoid the risk of diffusion. Furthermore, through the relatively complete sampling of the tumor mass, it is helpful to fully understand the immune microenvironment. Description of the Drawings

[0019] Figure 1 It is a schematic diagram of the overall structure of the device of the present invention;

[0020] Figure 2 It is a schematic sectional view of the device of the present invention;

[0021] Figure 3 It is a schematic front view of the device of the present invention;

[0022] Figure 4 It is an exploded schematic diagram of the device of the present invention;

[0023] Figure 5 It is a schematic diagram of the needle rod structure of the present invention;

[0024] Figure 6 It is a schematic diagram of the structure of the cutting and sampling assembly of the present invention.

[0025] In the figure: 1. Cutting and sampling assembly; 101. Hollow cam; 102. Side groove; 103. Sampling port; 104. End groove; 105. End sliding pin; 106. Cutting knife; 2. Sampler; 3. Flange; 4. Limit ring; 5. Side hole; 6. Gantry; 7. Side sliding pin; 8. Spring I; 9. Middle hole; 10. Needle rod; 11. Piston; 12. Needle handle; 13. Middle ring; 14. Spring II. Specific embodiments

[0026] The following further describes in detail the implementation manners of the present invention in conjunction with the accompanying drawings and embodiments. The following embodiments are used to illustrate the present invention, but cannot be used to limit the scope of the present invention

[0027] Please refer to Figures 1 to 6 , the present invention provides a technical solution: a sampling device for immune infiltration, including a cutting and sampling assembly 1, the cutting and sampling assembly 1 includes a hollow cam 101, a side groove 102, a sampling port 103, an end groove 104, an end sliding pin 105 and a cutting knife 106. A cavity is provided inside the hollow cam 101 along the axial direction, and a side groove 102 is opened on the side surface of the hollow cam 101. A sampling port 103 is opened in the middle of the end surface of the hollow cam 101, and end grooves 104 are opened on both sides of the end surface of the hollow cam 101. End sliding pins 105 are slidably installed corresponding to the inside of the two end grooves 104, and the end sliding pins 105 are integrally fixed to the end planes of the two cutting knives 106. The side groove 102 opened on the side surface of the hollow cam 101 is a "W"-shaped closed ring structure, and the end groove 104 opened on the end surface of the hollow cam 101 is a two-stage involute structure;

[0028] The specific operation is as follows. The medical staff holds the housing of the sampler 2 and inserts it into the patient's body through the stoma, so that the flange 3 at the end of the sampler 2 is closely attached to the tumor surface for positioning. When in use, the medical staff drives the needle rod 10 to move in a guiding manner by the limiting ring 4 located in the cavity of the sampler 2 by pressing the needle handle 12. Thanks to the structural design that the spring coefficient of the second spring 14 at the middle ring 13 in this application is greater than that of the first spring 8 at the gantry 6, on the one hand, it makes the first spring 8 at the gantry 6 easier to be compressed than the second spring 14 during the movement of the needle rod 10. Therefore, during the first-stage movement of the needle rod 10, the first spring 8 at the gantry 6 is first compressed, and then the needle rod 10 drives the sliding pins on the inner sides of both ends of the gantry 6 to slide in the circumferential side grooves 102 of the hollow cam 101, thereby converting the linear movement of the gantry 6 driven by the needle rod 10 into the rotational movement of the hollow cam 101. Further, the hollow cam 101 drives the cutting knives 106 on both sides of the flange 3 of the sampler 2 to expand synchronously through the cooperation between the end grooves 104 provided at its end and the end sliding pins 105 of the corresponding cutting knives 106, exposing the sampling port 103 in the middle of the end face of the hollow cam 101. After that, during the second-stage movement of the needle rod 10, the second spring 14 at the middle ring 13 is compressed later, so that the needle rod 10 drives the piston 11 to move in the hollow cavity of the hollow cam 101, and sprays the medical liquid such as disinfectant or anesthetic pre-filled in the cavity of the hollow cam 101 onto the sampling position through the sampling port 103, thereby realizing the disinfection and local anesthesia of the patient's lesion site. This application not only can convert the linear movement of the gantry 6 driven by the needle rod 10 into the rotational movement of the hollow cam 101 through the structural design of providing side grooves 102 and end grooves 104 corresponding to the circumference and end of the hollow cam 101, and further controls the synchronous opening and closing of the cutting knives 106 on both sides of the flange 3 through the rotational movement of the hollow cam 101, thereby realizing the opening and closing control of the sampling port 103 at the end of the sampler 2, but also through the hollow design in the middle of the hollow cam 101, the internal cavity of the hollow cam 101 can be pre-filled with the required medical liquid. Through the pumping of the piston 11 driven by the needle rod 10 in the cavity of the hollow cam 101, the medical liquid is sprayed onto the patient's lesion site to meet the functional requirements. The structure is compact and the linkage cooperation between components is strong;

[0029] Please refer to Figures 2 to 5, the hollow cam 101 is rotatably installed at the inner bottom end of the sampler 2, and the central axis of the inner cavity of the sampler 2 coincides with the axis direction of the hollow cam 101. A flange 3 with a ring structure is integrally fixed to the outer edge of the end of the sampler 2, and through holes for slidingly cooperating with the corresponding cutting knives 106 are provided on both sides of the flange 3. A limiting ring 4 is fixedly installed in the middle of the sampler 2, and side holes 5 are symmetrically opened on both sides of the limiting ring 4. A gantry 6 is slidably installed inside the side holes 5 on both sides, and side sliding pins 7 for cooperating with the side grooves 102 opened on the side of the hollow cam 101 are provided inside the end of the gantry 6. And a first spring 8 for elastically cooperating with the gantry 6 is provided above the side hole 5. The side sliding pin 7 inside the end of the gantry 6 drives the hollow cam 101 to rotate by itself through the cooperation with the side groove 102. During the rotation of the hollow cam 101, the end grooves 104 on both sides of the end surface drive the two cutting knives 106 to open and close synchronously through the cooperation with the corresponding end sliding pins 105. A central hole 9 is penetrated through the middle of the limiting ring 4, and a needle rod 10 is slidably installed inside the central hole 9. A piston 11 is connected to the bottom end of the needle rod 10, and the piston 11 is tightly fitted with the inner wall of the cavity of the hollow cam 101 through a sealing ring. And a needle handle 12 is fixedly connected to the top end of the needle rod 10. A middle ring 13 is fixedly installed in the middle of the needle rod 10, and the middle ring 13 is elastically cooperated with the end plane of the gantry 6 through a second spring 14. And the elastic coefficient of the second spring 14 is greater than that of the first spring 8;

[0030] The specific operation is as follows. On the other hand, during the reset process of the needle rod 10, the second spring 14 at the middle ring 13 will be reset first because its elastic coefficient is greater than that of the first spring 8 at the gantry 6. Therefore, when the needle rod 10 drives the piston 11 to reset inside the cavity of the hollow cam 101 under the elastic force of the second spring 14, a negative pressure will be generated inside the cavity of the hollow cam 101 where the liquid medicine has been emptied, so that the lesion located at the flange 3 at the end of the sampler 2 is sucked into the sampling port 103 under the negative pressure. Then, under the elastic force of the first spring 8, the gantry 6 drives the hollow cam 101 to rotate and reset through the end sliding pin 105, so that the cutting knives 106 on both sides of the flange 3 close towards each other, realizing the cutting and breaking of the root of the lesion sucked into the sampling port 103. On the one hand, in this application, the generation of negative pressure does not rely on an external negative pressure pipeline system, but on the generation of negative pressure during the reset process of the piston 11 inside the cavity of the hollow cam 101, realizing the negative pressure adsorption of the tumor mass. And on the other hand, through the rotation and reset of the hollow cam 101, the closing and cutting of the cutting knives 106 on both sides of the flange 3 are driven, and a relatively complete tumor mass that can be used for observation can be taken from the tumor mass. The sampling is complete and the risk of diffusion is avoided. Furthermore, through the relatively complete sampling of the tumor mass, it is helpful to fully understand the immune microenvironment.

[0031] In summary, when using the sampling device for immune infiltration, medical staff hold the housing of the sampler 2 and insert it into the patient's body through the stoma, so that the flange 3 part at the end of the sampler 2 is closely attached to the surface of the tumor for positioning. When in use, medical staff drive the needle rod 10 to move under the guidance of the limit ring 4 in the cavity of the sampler 2 by pressing the needle handle 12. Thanks to the structural design in this application that the elastic coefficient of the second spring 14 at the middle ring 13 is greater than that of the first spring 8 at the gantry 6, on the one hand, it makes the first spring 8 at the gantry 6 easier to be compressed than the second spring 14 during the movement of the needle rod 10. Therefore, during the first-stage movement of the needle rod 10, the first spring 8 at the gantry 6 is first compressed, and then the needle rod 10 drives the sliding pins on the inner sides of both ends of the gantry 6 to slide in the circumferential side grooves 102 of the hollow cam 101, thereby converting the linear movement of the gantry 6 driven by the needle rod 10 into the rotational movement of the hollow cam 101. Further, the hollow cam 101 drives the cutting knives 106 on both sides of the flange 3 of the sampler 2 to expand synchronously through the cooperation of the end groove 104 provided at the end and the end sliding pin 105 of the corresponding cutting knife 106, exposing the sampling port 103 in the middle of the end face of the hollow cam 101. After that, during the second-stage movement of the needle rod 10, the second spring 14 at the middle ring 13 is compressed later, so that the needle rod 10 drives the piston 11 to move in the hollow cavity of the hollow cam 101, and sprays medical liquid such as disinfectant or anesthetic pre-filled in the cavity of the hollow cam 101 onto the sampling position through the sampling port 103, thereby realizing the disinfection and local anesthesia of the patient's lesion site. This application not only can convert the linear movement of the gantry 6 driven by the needle rod 10 into the rotational movement of the hollow cam 101 through the structural design of providing side grooves 102 and end grooves 104 at the circumference and end of the hollow cam 101 respectively, and further controls the synchronous opening and closing of the cutting knives 106 on both sides of the flange 3 through the rotational movement of the hollow cam 101, thereby realizing the opening and closing control of the sampling port 103 at the end of the sampler 2, but also through the hollow design in the middle of the hollow cam 101, the internal cavity of the hollow cam 101 can be pre-filled with the required medical liquid, and through the pumping of the piston 11 driven by the needle rod 10 in the cavity of the hollow cam 101, the medical liquid is sprayed onto the patient's lesion site to meet the functional requirements. The structure is compact and the linkage cooperation between components is strong. On the other hand, during the reset process of the needle rod 10, the second spring 14 at the middle ring 13 will be reset first because its elastic coefficient is greater than that of the first spring 8 at the gantry 6. Therefore, when the needle rod 10 drives the piston 11 to reset inside the cavity of the hollow cam 101 under the elastic force of the second spring 14, a negative pressure will be generated in the internal cavity of the hollow cam 101 where the liquid medicine has been emptied, so that the lesion positioned at the flange 3 at the end of the sampler 2 is sucked into the sampling port 103. Then, the gantry 6 drives the hollow cam 101 to rotate and reset through the end sliding pin 105 under the elastic force of the first spring 8, and further makes the cutting knives 106 on both sides of the flange 3 close towards each other, realizing the cutting and breaking of the root of the lesion sucked into the sampling port 103.On the one hand, the generation of negative pressure in this application does not rely on an external negative pressure pipeline system, but on the generation of negative pressure during the reset process of the piston 11 inside the cavity of the hollow cam 101, realizing the negative pressure adsorption of the tumor mass. On the other hand, the rotation and reset of the hollow cam 101 drive the closing and cutting of the cutting knives 106 on both sides of the flange 3, enabling the removal of a relatively complete tumor mass for observation from the tumor mass. The sampling is complete, avoiding the risk of spread. Furthermore, the relatively complete tumor mass sampling helps to fully understand the immune microenvironment.

[0032] The embodiments of the present invention are given for purposes of illustration and description, and are not exhaustive or limit the invention to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are chosen and described to better explain the principles of the invention and its practical application, and to enable those of ordinary skill in the art to understand the invention and design various implementations with various modifications suitable for specific purposes.

Claims

1. A sampling device for immune infiltration, comprising a cutting sampling assembly (1), characterized in that: The cutting sampling assembly (1) comprises a hollow cam (101), a side groove (102), a sampling port (103), an end groove (104), an end sliding pin (105) and a cutting knife (106); a cavity is provided inside the hollow cam (101) along the axial direction, and a side groove (102) is provided on the side of the hollow cam (101); a sampling port (103) is provided in the middle of the end surface of the hollow cam (101), and end grooves (104) are provided on both sides of the end surface of the hollow cam (101); end sliding pins (105) are slidably installed inside the end grooves (104) on both sides, and the end sliding pins (105) are integrally fixed to the end planes of the cutting knives (106) on both sides.

2. A sampling device for immune infiltration according to claim 1, characterized in that: The side groove (102) provided on the side surface of the hollow cam (101) is a "W"-shaped closed annular structure, and the end groove (104) provided on the end surface of the hollow cam (101) is a two-stage involute structure.

3. A sampling device for immune infiltration according to claim 2, characterized in that: The hollow cam (101) is rotatably mounted on the inner bottom end of the sampler (2), and the central axis of the inner cavity of the sampler (2) coincides with the axial direction of the hollow cam (101).

4. A sampling device for immune infiltration according to claim 3, characterized in that: A flange (3) with an annular structure is integrally fixed to the outer edge of the end of the sampler (2), and through holes for slidingly cooperating with corresponding cutting knives (106) are provided on both sides of the flange (3).

5. A sampling device for immune infiltration according to claim 4, characterized in that: A limiting ring (4) is fixedly installed at the middle end of the sampler (2), and side holes (5) are symmetrically provided on both sides of the limiting ring (4).

6. A sampling device for immune infiltration according to claim 5, characterized in that: A gantry (6) is slidably installed inside the side holes (5) on both sides, and a side sliding pin (7) is provided on the inner side of the end of the gantry (6) to match the side groove (102) opened on the side of the hollow cam (101), and a spring (8) is provided above the side hole (5) to elastically match the gantry (6).

7. A sampling device for immune infiltration according to claim 6, characterized in that: The side sliding pin (7) on the inner side of the end of the gantry (6) drives the hollow cam (101) to rotate by cooperating with the side groove (102), and during the rotation of the hollow cam (101), the end grooves (104) on both sides of the end surface drive the cutting knives (106) on both sides to open and close synchronously by cooperating with the corresponding end sliding pins (105).

8. A sampling device for immune infiltration according to claim 7, characterized in that: A central hole (9) is provided through the middle of the limiting ring (4), and a needle rod (10) is slidably mounted inside the central hole (9).

9. A sampling device for immune infiltration according to claim 8, characterized in that: The bottom end of the needle rod (10) is connected to a piston (11), and the piston (11) is tightly matched with the inner wall of the hollow cam (101) cavity through a sealing ring, and the top end of the needle rod (10) is fixedly connected to a needle handle (12).

10. A sampling device for immune infiltration according to claim 9, characterized in that: A middle ring (13) is fixedly installed in the middle of the needle rod (10), and the middle ring (13) is elastically matched with the end plane of the gantry (6) through the second spring (14), and the elastic coefficient of the second spring (14) is greater than that of the first spring (8).

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