Reagent bottle

Abstract

A reagent bottle comprises a sample hole and a plurality of reagent holes, and the sample hole is used for accommodating a to-be-tested sample and providing a reaction place for the to-be-tested sample and a test card strip; the plurality of reagent holes are used for providing a reaction place for a plurality of test steps of enzyme-linked immunosorbent assay of a single test card strip attached with a sample to be tested; one reagent hole provides a reaction place for one test step, and the reagent hole contains a reagent required by the corresponding test step. The combination of one sample hole and a plurality of reagent holes is used for providing a place for enzyme-linked immunosorbent assay of a single to-be-tested sample and a single test card strip, so that the reagent bottle can be used for enzyme-linked immunosorbent assay of a single animal sample; the reagent bottle is designed to be capable of testing a single animal sample, and the reagent bottle can be made into a disposable consumable, so that the risk of sample cross contamination caused by repeated use is avoided; reagents are preset in the reagent bottles, so that the step of adding the reagents is omitted during testing, and the testing efficiency can be improved.

Description

Technical Field

[0001] The invention relates to the technical field of immunoassay, and in particular to a reagent bottle for enzyme-linked immunosorbent assay. Background Art

[0002] Currently, the enzyme-linked immunosorbent assay (ELISA) available on the market all adopts the form of 96-well ELISA plates, and the test plates are consumed according to the number of tested animal samples; for the pet market, single animal sample tests are mostly carried out, and the traditional 96-well ELISA plates are extremely unsuitable. At the same time, the traditional 96-well ELISA plates are used to test multiple animal samples. There is a risk of mutual contamination during the testing of multiple animal samples. For example, the liquid left in the original empty position after the test may cause risks to other untested wells. If the tested animal samples contain zoonotic diseases, it will pose a biological risk threat to both the operator and the storage environment. Summary of the invention

[0003] The present invention provides a reagent bottle, which is used to solve the problems of single animal sample testing adapted to the pet market and the risk of contamination.

[0004] In one embodiment, a reagent bottle is provided, the reagent bottle comprising: a sample hole and a plurality of reagent holes, the sample hole is used to accommodate a sample to be tested and provide a reaction site for the sample to be tested and a test card strip; the plurality of reagent holes are used to provide a reaction site for a plurality of test steps of an enzyme-linked immunosorbent assay performed on a test card strip attached with the sample to be tested;

[0005] Among them, one of the reagent holes provides a reaction site for one of the test steps, and the reagent hole contains the reagents required for the corresponding test step.

[0006] In one embodiment, the reagent hole provided with the reagent is also provided with a sealing structure for sealing the reagent, and the sealing structure can be punctured or removed.

[0007] In one embodiment, the sealing structure includes a membrane that can be pierced by the test card strip.

[0008] In one embodiment, the sample well and the plurality of reagent wells are distributed on a circular trajectory of the reagent bottle.

[0009] In one embodiment, the reagent bottle further includes a slot for inserting the test card strip for completing the enzyme-linked immunosorbent assay.

[0010] In one embodiment, the reagent bottle is provided with a test result comparison reference map, which is arranged side by side with the slot, and the test result comparison reference map is used for color comparison with the test card strip that completes the enzyme-linked immunosorbent assay to obtain the test result.

[0011] In one embodiment, a press-jump device is further included, and the press-jump device includes a fixed end and a jump end, the fixed end is arranged on the reagent bottle, the jump end is movably connected to the fixed end, and the jump end is used to fix the test card strip. After the jump end is pressed, it will rise and fall and rotate relative to the fixed end to drive the test card strip to be inserted into the sample hole and the multiple reagent holes in sequence.

[0012] In one embodiment, a mounting hole is provided in the middle of the reagent bottle, the sample hole and the plurality of reagent holes are surrounded by the mounting hole, the fixed end is disposed in the mounting hole, and the jump end is exposed from the mounting hole.

[0013] In one embodiment, the jump end is provided with a clamping portion, and the clamping portion is used to clamp and fix the test card strip.

[0014] In one embodiment, the reagent bottle includes 6 reagent holes.

[0015] According to the reagent bottle for enzyme-linked immunosorbent assay (ELISA) of the above-mentioned embodiment, a sample hole and a plurality of reagent holes are provided on the reagent bottle. The combination of a sample hole and a plurality of reagent holes is used to provide a place for performing ELISA on a single sample to be tested and a single test card strip. A reagent required for an ELISA test step is provided in the reagent hole, so that the reagent bottle can perform ELISA on a single animal sample. Since the reagent bottle is designed to test a single animal sample and its hole position is relatively small, the reagent bottle can be made into a disposable consumable and not reused, thus avoiding the risk of sample cross-contamination caused by repeated use. Furthermore, the reagent is pre-installed in the reagent bottle, which eliminates the step of adding reagents during testing, thereby improving test efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of a reagent bottle in an embodiment;

[0017] Figure 2 A top view of a reagent bottle in one embodiment;

[0018] Figure 3 This is a schematic diagram of the structure of a reagent bottle in an embodiment;

[0019] Figure 4 A top view of a reagent bottle in one embodiment;

[0020] Figure 5 This is a schematic diagram of the structure of a reagent bottle in an embodiment;

[0021] Figure 6 This is a schematic diagram of the structure of a reagent bottle in an embodiment;

[0022] Figure 7This is a schematic diagram of the structure of a reagent bottle in an embodiment;

[0023] Figure 8 This is a schematic diagram of the structure of a reagent bottle with a hidden upper cover in an embodiment;

[0024] Fig. 9 This is a schematic diagram of the structure of a reagent bottle in an embodiment;

[0025] The reference numerals are as follows:

[0026] 10-reagent bottle, 11-sample hole, 12-reagent hole, 13-test result comparison reference chart, 14-slot, 15-installation hole.

[0027] 20-test card strip;

[0028] 30 - press-jump device, 31 - fixed end, 32 - jump end, 321 - clamping part. DETAILED DESCRIPTION

[0029] The present invention is further described in detail below by specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments adopt associated similar element numbers. In the following embodiments, many detailed descriptions are for making the present application better understood. However, those skilled in the art can easily recognize that some features can be omitted in different situations, or can be replaced by other elements, materials, methods. In some cases, some operations related to the present application are not shown or described in the specification, this is to avoid the core part of the present application being overwhelmed by too much description, and for those skilled in the art, it is not necessary to describe these related operations in detail, and they can fully understand the related operations according to the description in the specification and the general technical knowledge in the art.

[0030] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various implementations. At the same time, the steps or actions in the method description can also be interchanged or adjusted in a manner that is obvious to those skilled in the art. Therefore, the various sequences in the specification and the drawings are only for the purpose of clearly describing a certain embodiment and are not meant to be a required sequence, unless otherwise specified that a certain sequence must be followed.

[0031] The serial numbers of the components in this document, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings).

[0032] In one embodiment, a reagent bottle is provided. The reagent bottle is a disposable consumable and is used for enzyme-linked immunosorbent assay of a single sample.

[0033] This reagent bottle is mainly used for enzyme-linked immunosorbent assay of animal samples. Generally, the number of animal samples tested is small, and the use of 96-well ELISA plates does not bring cost savings. Instead, there are problems of cross-contamination and low efficiency when testing multiple samples. In the process of adding reagents and samples, multiple samples are prone to splashing, overflowing, and adding the wrong wells, resulting in mutual contamination and interference among multiple tests.

[0034] In this embodiment, a reagent bottle for a single sample is used, and relatively fewer holes are set on the reagent bottle. The number of holes is sufficient to meet the needs of a single animal sample for ELISA testing, which can reduce the cost of the reagent bottle. This reagent bottle is used to test a single animal sample, which can completely avoid the problem of cross-contamination of multiple animal samples, and there is no problem of contamination of the traditional 96-well plate. In addition, reagents can be pre-set in the reagent bottle, and the step of adding reagents can be omitted during the test, and the test efficiency is higher.

[0035] Please refer to Figure 1 and Figure 2 The reagent bottle 10 of this embodiment may include a sample hole 11 and multiple reagent holes 12, wherein the number of reagent holes 12 can be the maximum value of the holes required for enzyme-linked immunosorbent assay of different samples and different items. For example, the reagent holes 12 may include 6 holes, and a total of 7 holes are arranged on the reagent bottle 10, which can meet the requirements of enzyme-linked immunosorbent assay of most items of most animal samples.

[0036] The sample well 11 is used to add and accommodate samples, and provides a reaction site for the sample to be tested and the test card strip 20. The multiple reagent wells 12 are used to provide reaction sites for multiple test steps of the enzyme-linked immunosorbent assay performed on a single test card strip 20 with the sample to be tested, wherein one reagent well 12 provides a reaction site for one test step. The sample well 11 contains reagents required for the reaction between the sample to be tested and the test card strip 20, and the reagent well 12 contains reagents required for the corresponding test steps.

[0037] The principle steps of performing an ELISA test on a single sample to be tested and a single test card strip 20 are as follows:

[0038] First, add the sample into the sample well 11, and then insert the test card strip 20 into the sample well 11, so that the sample to be tested and the reagent in the sample well 11 and the reagent on the test card strip 20 undergo a first test reaction;

[0039] After the first test reaction is completed, the test card strip 20 is inserted into a reagent well 12 to perform a second test reaction, so that the test card strip 20 with the sample to be tested reacts with the reagent in the reagent well 12;

[0040] After the second step test reaction is completed, the test card strip 20 is inserted into another reagent well 12, and the test card strip 20 is inserted into multiple reagent wells 12 in sequence to complete the enzyme-linked immunosorbent assay;

[0041] After the test card strip 20 is subjected to the ELISA test, the reagent on the test card strip 20 will develop color, and then the test card strip 20 is compared with the test result with reference to FIG. 13 to obtain the ELISA test result of the sample to be tested.

[0042] Take the ELISA test performed on canines as an example:

[0043] The sample well 11 is pre-set with a diluent, the first reagent well 12 is pre-set with a cleaning solution, the second reagent well 12 is pre-set with a key reagent, the third reagent well 12 is pre-set with a cleaning solution, the fourth reagent well 12 is pre-set with a cleaning solution, the fifth reagent well 12 is pre-set with a colorimetric reagent, and the sixth reagent well 12 is pre-set with a termination reagent. In order to clearly distinguish the six reagent wells 12, the six reagent wells 12 are named the first to sixth reagent wells 12, respectively.

[0044] Step 1: During the ELISA test, the sample to be tested is first added to the sample well 11, and the diluent in the sample well 11 is mixed with the sample to be tested to dilute the sample to be tested; then the test card strip 20 is inserted into the sample well 11, and the diluted sample in the sample well 11 and the reagent on the test card strip 20 undergo the first test reaction;

[0045] Step 2: After the first step of the test reaction, the test card strip 20 is inserted into the first reagent well 12 for cleaning to remove the unattached and inadequately reacted sample on the test card strip 20;

[0046] Step 3: insert the tilted test card strip 20 into the second reagent hole 12, and the reagent combined with the sample on the test card strip 20 reacts with the key reagent;

[0047] Step 4: After the reaction is completed, the test card strip 20 is inserted into the third reagent well 12 for the first cleaning to remove the unattached and inadequately reacted reagents on the test card strip 20;

[0048] Step 5: After the first cleaning is completed, a second cleaning is performed, and the test card strip 20 is inserted into the fourth reagent hole 12 for a second cleaning to fully remove the unattached and inadequately reacted reagents on the test card strip 20;

[0049] Step 6: After the second cleaning, the test card strip 20 is inserted into the fifth reagent well 12, and the reagent of the test card strip 20 is incubated in the fifth reagent well 12 with the color developing reagent, so that the reagent on the test card strip 20 is colored.

[0050] Step 7: insert the color-developed test card strip 20 into the sixth reagent well 12. The sixth reagent well 12 contains a termination reagent, which is used to terminate the color development incubation of the test card strip 20, thereby ending the ELISA test.

[0051] In this embodiment, the reagent in a reagent well 12 is the reagent required for the corresponding test step. Different reagents are pre-stored in the reagent well 12 according to different test items, and the number of reagents is also related to the test steps. For example, if a test item involves a total of 5 steps and only 4 reagent wells 12 are needed, the fifth reagent well 12 and the sixth reagent well 12 may not be pre-stored with reagents.

[0052] In this embodiment, a sample hole 11 and multiple reagent holes 12 are provided on the reagent bottle 10, and reagents corresponding to the test items are respectively provided in the sample hole 11 and the reagent hole 12, so that one reagent bottle 10 can realize the enzyme-linked immunosorbent assay of a single sample to be tested and a single test card strip 20. The reagent bottle 10 is a disposable consumable and is not reused, thereby avoiding cross contamination between samples of different tests. The reagent bottle 10 with a built-in reagent saves the step of adding reagents and improves the detection efficiency.

[0053] In one embodiment, the reagent hole 12 pre-set with the reagent is provided with a sealing structure for sealing the reagent. The sample hole 11 is also provided with a reagent, and the sample hole 11 is also provided with a sealing structure for sealing the reagent. The sealing structure seals the reagent in the hole, so that the reagent pre-set in the reagent bottle 10 will not overflow during storage and transportation of the reagent bottle 10, and the reagent can also be isolated and protected to prevent the reagent from being contaminated or deteriorated.

[0054] The sealing structure can be pierced or removed so that the test card strip 20 can be inserted into the sample well 11 and the reagent well 12 to contact with the reagents to achieve reaction.

[0055] The sealing structure can be a puncturable structure such as a film, for example, the sealing structure is a tin foil layer. The tin foil layer has a good water-proof and air-proof protective effect, and the tin foil is also easy to be punctured, so that the test card strip 20 can puncture the sealing structure and be inserted into the sample hole 11 and the reagent hole 12 during the test.

[0056] The sealing structure may also be a removable structure such as a sealing cover, for example, the sealing structure is a plastic cover, which may be threaded or snap-fitted with the reagent bottle 10, and the plastic cover may also play a sealing and protective role. During the test, the plastic cover is first opened or removed, and then the test card strip 20 is inserted into the sample well 11 and the reagent well 12. The sealing structure of the plastic cover structure may also seal the reagent and prevent the test card strip 20 from being inserted into the test.

[0057] In one embodiment, the sample hole 11 and the multiple reagent holes 12 are distributed on a circular track of the reagent bottle 10. The sample hole 11 and the multiple reagent holes 12 can be arranged in sequence at equal intervals on a circular track. The test card strip 20 can be inserted into the sample hole 11 and the multiple reagent holes 12 in sequence by jumping, and the test card strip 20 can be automatically jumped and inserted.

[0058] If a manual test method of inserting the test card strip 20 is adopted, the mutual spacings between the sample well 11 and the plurality of reagent wells 12 may be equal or unequal.

[0059] The reagent bottle 10 may be a cylindrical or tubular structure, the sample hole 11 and the multiple reagent holes 12 are arranged on the same axial end face of the reagent bottle 10, and the depth direction of the sample hole 11 and the multiple reagent holes 12 is parallel to the axial direction of the reagent bottle 10. The reagent bottle 10 with a cylindrical or tubular structure can make the layout of the sample hole 11 and the multiple reagent holes 12 more compact, which is conducive to miniaturization of the reagent bottle 10 and convenient storage and transportation of the reagent bottle 10.

[0060] In one embodiment, the reagent bottle 10 can also be a straight strip, a folded strip or a curved strip structure, and the sample hole 11 and the multiple reagent holes 12 are distributed on a straight line track, a folded line track or a curved track of the reagent bottle 10. The test card strip 20 can also be inserted into the sample hole 11 and the multiple reagent holes 12 in sequence by linear movement, folded line movement or curved line movement to realize enzyme-linked immunosorbent assay.

[0061] In one embodiment, the sample well 11 and the multiple reagent wells 12 can also be distributed in a rectangular array, such as one sample well 11 and six reagent wells 12 can be divided into two rows and four columns, one row includes one sample well 11 and three reagent wells 12, and the other row includes three reagent wells 12. The sample well 11 and the multiple reagent wells 12 can also be arranged compactly, which is conducive to miniaturization of the reagent bottle 10 and convenient storage and transportation of the reagent bottle 10.

[0062] In one embodiment, the sample hole 11 and the reagent hole 12 are flat holes that match the shape and structure of the test card strip 20, and the cross-sections of the sample hole 11 and the reagent hole 12 (perpendicular to the direction in which the test card strip 20 is inserted) are rectangular or track-shaped structures. The sample hole 11 and the reagent hole 12 are configured as flat holes that match the shape and structure of the test card strip 20, which can reduce the space occupied by the sample hole 11 and the reagent hole 12, is conducive to miniaturization of the reagent bottle 10, and reduces material costs.

[0063] Please refer to Figure 3 and Figure 4 In one embodiment, the reagent bottle 10 further includes a slot 14, and the slot 14 is used to insert a test card strip 20 for completing an ELISA test. The sample well 11, the plurality of reagent wells 12, and the slot 14 are arranged in sequence, for example, the sample well 11, the plurality of reagent wells 12, and the slot 14 are arranged in sequence on a circular track, or the sample well 11, the plurality of reagent wells 12, and the slot 14 can also be arranged in sequence on a linear track. After the test card strip 20 is inserted into the sample well 11 and the plurality of reagent wells 12 in sequence to complete the ELISA test, it is inserted into the slot 14 next to the last reagent well 12.

[0064] The slot 14 includes an insertion opening parallel to the insertion direction and an opening perpendicular to the insertion direction. The insertion opening of the slot 14 is parallel to the openings of the sample well 11 and the plurality of reagent wells 12, so that the test card strip 20 can be sequentially inserted into the slot 14. The opening of the slot 14 is used to display the color-developing reagent on the test card strip 20, so as to realize manual or automatic visual reading of the color of the reagent on the test card strip 20 to determine the result of the enzyme-linked immunosorbent assay.

[0065] In one embodiment, the slot 14 includes a socket parallel to the insertion direction and a transparent window perpendicular to the insertion direction. The transparent window can be transparent glass or transparent plastic. The transparent window and the opening have the same function, which is used to display the colored reagent on the test strip 20.

[0066] Please refer to Figure 5 In one embodiment, the reagent bottle 10 is provided with a test result comparison reference 13, which is located on the side of the reagent bottle 10, and is adjacent to and arranged side by side with the opening of the slot 14. The test result comparison reference 13 is used to compare the color with the test card strip 20 that completes the enzyme-linked immunosorbent assay in the slot 14 to obtain the test result. The color comparison between the test result comparison reference 13 and the test card strip 20 can be achieved by manual or automated visual reading.

[0067] The test result comparison reference 13 is a long paper tape, and a plurality of color blocks are arranged along the length direction of the long paper tape. The test card strip 20 is a long card, and a plurality of reagents are arranged along the length of the long card. The test result comparison reference 13 corresponds one by one to the color blocks on the test card strip 20, so that the test card strip 20 inserted into the slot 14 can be intuitively compared with the test result comparison reference 13, which is beneficial to improve the comparison efficiency and comparison accuracy.

[0068] The test result comparison reference 13 can be fixed to the side of the reagent bottle 10 by pasting. The side of the reagent bottle 10 can also be formed with a test result comparison reference 13 by printing or spraying.

[0069] Please refer to Figures 6 to 9 In one embodiment, the reagent bottle 10 can be inserted into the sample well 11 and the reagent well 12 in sequence by pressing and jumping the test card strip 20. The reagent bottle 10 also includes a press-jump device 30, which is used to install and fix the test card strip 20. The press-jump device 30 can drive the test card strip 20 to be inserted into the sample well 11 and multiple reagent wells 12 on the reagent bottle 10 in sequence. If the reagent bottle 10 is also provided with a slot, the press-jump device 30 can also drive the test card strip 20 to be inserted into the slot 14.

[0070] The reagent bottle 10 may be a cylindrical structure, and a mounting hole 15 is provided in the middle of the axial side of the reagent bottle 10, and the sample hole 11 and the plurality of reagent holes 12 surround the mounting hole 15. If the reagent bottle 10 is also provided with a slot, the sample hole 11, the plurality of reagent holes 12 and the slot 14 surround the mounting hole 15. The sample hole 11, the plurality of reagent holes 12 and the slot 14 are arranged in sequence and equidistantly on a circular track.

[0071] The press jump device 30 is installed in the installation hole 15 , and the press jump device 30 can drive the test card strip 20 to jump the same angle each time, so as to insert the test card strip 20 into the sample well 11 , multiple reagent wells 12 and the slot 14 in sequence.

[0072] The press-jump device 30 may include a fixed end 31 and a jump end 32. The fixed end 31 is fixedly disposed in the mounting hole 15 of the reagent bottle 10. The jump end 32 is movably connected to the fixed end 31. The jump end 32 is exposed from the mounting hole 15. The jump end 31 can be detachably installed with the test card strip 20 to drive the test card strip 20 to jump.

[0073] The jumping principle of the pressing jump device 30 is the same or similar to the jumping principle of the pressing head of an automatic ballpoint pen. A plurality of card slots 151 are arranged on the inner side wall of the mounting hole 15 of the reagent bottle 10. The plurality of card slots 151 are arranged in equal intervals in sequence. Bosses are formed between adjacent card slots. The circumferential angle between the card slots is equal to the circumferential angle between adjacent holes of the sample hole 11 and the reagent hole 12. The fixed end 31 is a columnar structure. The upper end of the outer circumferential side surface of the fixed end 31 is distributed with a first connecting tooth facing downward. The lower end of the fixed end 31 is fixed to the middle position of the bottom surface of the mounting hole 15. An annular space is formed between the fixed end 31 and the mounting hole 15. The jump end 32 is a cylindrical structure. The outer circumferential side surface of the jump end 32 is provided with a plurality of inserts corresponding to the card slots one by one. The inserts can be inserted into the card slots or can be placed on the bosses. The connection between the inserts and the card slots and the bosses is used to realize the lifting and lowering of the jump end 32 relative to the fixed end 31. The lower end of the inner circumferential side surface of the jump end 32 is provided with a second connecting tooth, the second connecting tooth is adapted to the first connecting tooth, the first connecting tooth and the second connecting tooth are both unidirectionally inclined teeth, and the connection between the second connecting tooth and the first connecting tooth is used to drive the jump end 32 to rotate relative to the fixed end 31. The mounting hole 15 is also equipped with a reset structure such as a spring, and the reset structure is connected to the jump end 32 to drive the jump end 32 to reset and rise.

[0074] For the sake of aesthetics and convenience in pressing the jumping end 32 , an upper cover may be provided at the upper end of the jumping end 32 , and the upper cover will cover the fixed end 31 located inside the jumping end 32 .

[0075] The steps of using the pressing jump device 30 to drive the test card strip 20 to jump are as follows:

[0076] In the initial state, the test card strip 20 is located directly above the sample hole 11. Pressing the jump end 32 of the jump device 30 once will drive the test card strip 20 to be inserted into the sample hole 11.

[0077] After the first reaction is finished, the jump end 32 is pressed again, and the jump end 32 drives the test card strip 20 to be pulled out of the sample well 11 and jump a certain angle, so that the test card strip 20 is located above the first reagent well 12 (the reagent well 12 adjacent to the sample well 11);

[0078] The jump end 32 is pressed continuously at intervals, and finally the test card strip 20 is inserted into the slot 14 to complete the pressing operation.

[0079] The pressing jump device 30 can be pressed manually or automatically by automated equipment.

[0080] In one embodiment, the jump end 32 is provided with a clamping portion 321, and the clamping portion 321 is used to clamp and fix the test card strip 20. The clamping portion 321 can be a mounting groove, and the test card strip 20 is inserted into the mounting groove. The mounting groove can limit and fix the test card strip 20, especially the insertion and removal direction of the test card strip 20, so that the jump end 32 can drive the insertion and removal jump of the test card strip 20. And it can also drive the test card strip 20 to pierce the sealing structure on the sample hole 11 and / or the reagent hole 12.

[0081] The clamping portion 321 can be a detachable fixed block, and the fixed block and the jump end 32 can be clamped together to form a mounting groove when the fixed block and the jump end 32 are clamped together. The fixed block can fix the test card strip 20 in the mounting groove from the radial direction of the test card strip 20.

[0082] In one embodiment, the clamping portion 321 may also be a screw, a threaded hole is provided on the jump end 32 , and a through hole for fixing is provided on the test card strip 20 . The screw can also detachably fix the test card strip 20 to the jump end 32 .

[0083] In one embodiment, the push jump device 30 may also be other structures, for example, the push jump device 30 includes a lifting component and a rotating component, the lifting component and the rotating component are operated separately, the lifting component is located at the lower end, the rotating component is installed on the lifting component, the lifting component drives the rotating component and the test card strip 20 to rise and fall, and the rotating component is used to drive the test card strip 20 to rotate. During operation, the lifting component can be operated by pressing or other methods to drive the test card strip 20 to rise and fall, and then the test card strip 20 can be operated to rotate by rotating.

[0084] The lifting assembly may include an upper cylinder and a lower cylinder that can be lifted and lowered relative to each other, and the rotating assembly may include a chassis and a rotating disk that can rotate relative to each other, the chassis is fixedly connected to the upper cylinder, and the test card strip 20 is installed and fixed on the rotating disk. Components such as springs and clamping structures may also be provided between the upper cylinder and the lower cylinder of the lifting assembly to achieve the lowering limit and automatic rising and resetting of the test card strip 20. A limiting structure such as clamping teeth may be provided between the chassis and the rotating disk of the rotating assembly to enable the rotating disk to rotate a certain angle each time.

[0085] The above specific examples are used to illustrate the present invention, which is only used to help understand the present invention and is not intended to limit the present invention. For those skilled in the art, according to the concept of the present invention, some simple deductions, modifications or substitutions can be made.

Claims

1. A reagent bottle, characterized in that, the reagent bottle (10) includes: a sample hole (11) and a plurality of reagent holes (12), the sample hole (11) is used for accommodating a sample to be tested and providing a reaction site for the sample to be tested and the test strip (20); the plurality of reagent holes (12) are used for providing reaction sites for a plurality of test steps of performing an enzyme-linked immunosorbent assay on one test strip (20) attached with the sample to be tested; wherein, one reagent hole (12) provides a reaction site for one test step, and the reagent hole (12) accommodates the reagent required for the corresponding test step.

2. The reagent bottle according to claim 1, characterized in that, the reagent hole (12) containing the reagent is further provided with a sealing structure for sealing the reagent, and the sealing structure can be punctured or removed.

3. The reagent bottle according to claim 2, characterized in that, the sealing structure includes a film that can be punctured by the test strip (20).

4. The reagent bottle according to claim 1, characterized in that, the sample hole (11) and the plurality of reagent holes (12) are distributed on a circumferential track of the reagent bottle (10).

5. The reagent bottle according to claim 1, characterized in that, the reagent bottle (10) further includes a slot (14), and the slot (14) is used for inserting the test strip (20) that has completed the enzyme-linked immunosorbent assay.

6. The reagent bottle according to claim 5, characterized in that, the reagent bottle (10) is provided with a test result comparison reference diagram (13), the test result comparison reference diagram (13) is arranged side by side with the slot (14), and the test result comparison reference diagram (13) is used for color comparison with the test strip (20) that has completed the enzyme-linked immunosorbent assay to obtain a test result.

7. The reagent bottle according to claim 1, characterized in that, it further includes a pressing and jumping device (30), the pressing and jumping device (30) includes a fixed end (31) and a jumping end (32), the fixed end (31) is arranged on the reagent bottle (10), the jumping end (32) is movably connected to the fixed end (31), the jumping end (32) is used for fixing the test strip (20), and after the jumping end (32) is pressed, it will move up and down and rotate relative to the fixed end (31) to drive the test strip (20) to be inserted into the sample hole (11) and the plurality of reagent holes (12) in sequence.

8. The reagent bottle according to claim 7, characterized in that, a mounting hole (15) is provided in the middle of the reagent bottle (10), the sample hole (11) and the plurality of reagent holes (12) surround the mounting hole (15), the fixed end (31) is arranged in the mounting hole (12), and the jumping end (32) is exposed outside the mounting hole (15).

9. The reagent bottle according to claim 7, characterized in that, the jumping end (32) is provided with a clamping portion (321), and the clamping portion (321) is used for clamping and fixing the test strip (20).

10. The reagent bottle according to any one of claims 1 to 9, Characterized in that, The reagent bottle (10) includes six of the reagent holes (12).

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