New bunyavirus antigen-antibody detection kit

Abstract

The invention discloses a new bunyavirus antigen-antibody detection kit, relates to the field of new bunyavirus detection, and solves the problem that the detection result is influenced by more manual intervention in the prior art, the new bunyavirus antigen-antibody detection kit comprises a device shell, a swab is inserted in the device shell, a notch is formed in the device shell, a detection piece is arranged in the notch, and the swab is inserted into the detection piece. A reagent tube is arranged above the detection piece, and a liquid outlet tube is mounted at the bottom end of the reagent tube; the mixing assembly is used for fully mixing reagents in the swab and the reagent tube, and the mixing assembly is mounted right above the reagent tube; through mutual cooperation of the mixing assembly, the liquid inlet assembly and the moving assembly, the swab and the reagent are mixed through a mechanical structure, and the mixed solution is automatically dripped and guided, so that in the detection process, excessive manual intervention is not needed, automatic detection can be performed, and the effect of more accurate detection result is achieved.

Description

Technical Field

[0001] The present invention relates to the field of detection of novel bunyaviruses, and specifically to a kit for detecting novel bunyavirus antigen and antibody. Background Art

[0002] The novel bunyavirus is a new type of virus isolated from ticks, mainly transmitted through ticks, or infected by contact with the blood and hemorrhagic secretions of patients. The main symptoms of this virus include persistent high fever, general muscle soreness, superficial lymph node enlargement, abdominal pain, diarrhea, etc., and may lead to multiple organ failure and death in severe cases. Currently, the existing kits for detecting novel bunyavirus antigen and antibody mostly rely on traditional colloidal gold chromatography or ELISA methods. Although the detection of novel bunyaviruses has been achieved to a certain extent, there is still room for improvement in terms of operation convenience, detection efficiency, and result accuracy. In particular, when the existing kits detect the virus, there are too many manual intervention processes, such as the mixing of swabs and reagents. In addition, the mixed solution needs to be manually dropped into the drip tank on the test strip. Manual mixing will result in incomplete mixing, and manually dropping the solution will make it difficult to control the amount of the dropped solution. Therefore, more manual interventions will lead to inaccurate detection results. Summary of the Invention

[0003] The purpose of the present invention is to provide a kit for detecting novel bunyavirus antigen and antibody to solve the problems raised in the above background art.

[0004] To achieve the above purpose, the present invention provides the following technical solutions: A kit for detecting novel bunyavirus antigen and antibody, comprising a device housing, a mixing assembly, a liquid inlet assembly, and a moving assembly. A swab is inserted into the device housing. The swab is inserted inside the device housing, which is convenient for carrying the swab and can ensure the cleanliness of the swab at the same time. And a notch is provided on the device housing, and a test strip is arranged in the notch. The position of the notch is on the side of the device housing, which is convenient for putting the test strip into the device housing and can facilitate taking out the test strip for observation after the detection. A reagent tube is arranged above the test strip, and a liquid outlet tube is installed at the bottom end of the reagent tube. A drip tank and an observation tank are arranged on the test strip. The reagent tube is installed directly above the drip tank. The mixing assembly is used to fully mix the swab and the reagent inside the reagent tube. The mixing assembly is installed directly above the reagent tube. The liquid inlet assembly is used to transport the mixed reagent solution inside the reagent tube to the test strip. The liquid inlet assembly is installed below the reagent tube. The moving assembly is used to drive the test strip to move. The moving assembly is arranged in cooperation with the liquid inlet assembly, and the moving assembly is installed at the bottom end of the device housing.

[0005] Preferably, the mixing assembly includes a rotating block rotatably connected to the device housing. A clamping member for clamping the swab is mounted on the rotating block. The swab is clamped by the clamping member so that the swab can be stably inserted into the reagent tube. A gear ring fixedly connected to the outer side of the rotating block and rotatably connected to the device housing is provided. A spur gear is meshed and driven on the outer side of the gear ring. A rotating shaft rotatably connected to the device housing is fixedly connected to the center of the spur gear. A driven wheel is fixedly connected to the end of the rotating shaft. A belt is connected to the outer side of the driven wheel in a transmission manner. A driving wheel is connected to the belt in a transmission manner. A driving member is connected to the center of the driving wheel. Through the driving force provided by the driving member and the transmission effect of the mechanism, the rotating block can be rotated. Through the rotation of the rotating block, the clamping member drives the swab to rotate inside the reagent tube, so as to uniformly mix the sample adsorbed on the swab in the reagent in the reagent tube.

[0006] Preferably, the clamping member includes a sliding sleeve inserted into the rotating block. A slot adapted to the sliding sleeve is provided on the rotating block, so that the sliding sleeve and the rotating block are detachably connected, which is convenient for taking the sliding sleeve. Two groups of clamping blocks are symmetrically mounted in the sliding sleeve. Both groups of clamping blocks are slidably connected to the sliding sleeve. A spring fixedly connected to the sliding sleeve is fixedly connected to the clamping block. Through the action of the spring, the two groups of clamping blocks tightly clamp the swab. A first groove located on both sides of the sliding sleeve is provided on the end face of the rotating block. Through the design of the first groove, it is convenient to remove the sliding sleeve from the rotating block.

[0007] Preferably, the liquid inlet assembly includes a support sheet installed above the detection sheet. The support sheet is convenient for protecting the detection sheet to avoid damage. A connecting pipe is fixedly connected to the end of the support sheet. A plurality of liquid outlet holes are provided on the connecting pipe. A thimble is fixedly connected to the end of the connecting pipe. Through the movement of the thimble, the thimble is inserted into the reagent tube. After that, the mixed reagent in the reagent tube will flow into the connecting pipe through the thimble, and then flow out from the liquid outlet holes and flow to the detection sheet. The thimble is communicated with the connecting pipe, and the thimble is inserted on the liquid outlet pipe.

[0008] Preferably, the moving assembly includes a sliding frame sleeved on the outer side of the detection sheet. The sliding frame is slidably connected to the device housing. The detection sheet is placed on the sliding frame. Through the movement of the sliding frame, the detection sheet presses the thimble to move. A threaded block is fixedly connected to the sliding frame. A reciprocating lead screw is threadedly connected to the threaded block. A rotation limiting member is connected to the end of the reciprocating lead screw. The function of the rotation limiting member is that during the mixing of the reagent, the reciprocating lead screw will not rotate. On the contrary, after the mixing is completed, the reciprocating lead screw will rotate to make the sliding frame move. The rotation limiting member is connected to the driving member.

[0009] Preferably, the rotation limiting member includes a rotating plate connected to the driving member. The end of the rotating plate is rotatably connected to a ratchet gear fixedly connected to the reciprocating lead screw. A ratchet pawl is engaged with the outside of the ratchet gear. A rotating column fixedly connected to the ratchet pawl is rotatably connected to the rotating plate. A torsion spring fixedly connected to the rotating column and fixedly connected to the rotating plate is provided. During the process of mixing the swab and the reagent, the ratchet pawl and the ratchet gear will be disengaged from each other. Therefore, the rotation of the rotating plate will not drive the rotation of the ratchet gear, and thus the reciprocating lead screw will not rotate in this state. Conversely, the reciprocating lead screw will rotate.

[0010] Preferably, the driving member includes a motor fixed inside the device housing. The output end of the motor is fixedly connected to a coupling. The end of the coupling is fixedly connected to a drive shaft fixedly connected to the driving wheel. The drive shaft is also fixedly connected to the rotating plate, providing a stable driving force for the rotation of the reciprocating lead screw and the rotation of the rotating shaft.

[0011] Preferably, a sliding plate is slidably connected to the device housing. A clamp is fixedly connected to the sliding plate. The clamp is clamped with the reagent tube. The reagent tube is fixed by the clamp, which is convenient for pulling out the reagent tube for replacement. Second grooves are provided at both ends of the sliding plate on the device housing, facilitating the pulling out of the sliding plate.

[0012] Preferably, an anti-slip pad is provided at the position where the clamping block contacts the swab. Through the setting of the anti-slip pad, the friction between the swab and the clamping block is increased, which is convenient for improving the stability of the swab and preventing relative sliding between the clamping block and the swab, thus affecting the mixing efficiency of the swab and the reagent.

[0013] Preferably, a top cover is provided at the top of the device housing. Through the design of the top cover, the swab and the parts in the device can be protected.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: In the present invention, through the mixing component, during the mixing process, the motor is controlled to rotate forward, and then the rotation of the motor is combined with the mechanical transmission to make the rotating block rotate. The clamping member on the rotating block will clamp and fix the swab. Therefore, when the rotating block rotates, the clamping member will drive the swab to rotate. Through the control of the mechanical structure, the swab rotates at a high speed in the reagent tube, thereby achieving a more thorough mixing effect of the swab and the reagent.

[0015] Through the mutual cooperation of the liquid inlet assembly and the moving assembly, after the mixing is completed, the detection piece drives the liquid inlet assembly to move under the action of the moving assembly, and then the mixed solution is diverted to the liquid dropping groove on the detection piece through the action of the thimble. Therefore, the mechanical movement of the thimble is controlled by the motor to ensure that the amount of the solution dropped into the liquid dropping groove on the detection piece is fixed, achieving the effect that the amount of the dropped solution is convenient to control.

[0016] Through the mutual cooperation of the connecting pipe and the liquid outlet pipe, after the solution diversion is completed, the cooperation between the connecting pipe and the liquid outlet pipe in the liquid inlet assembly can automatically close the solution flow channel, thus avoiding the continuous flow of the mixed solution and achieving the effect that the device will not be contaminated and can be reused. Brief Description of the Drawings

[0017] Figure 1 is a schematic diagram of the overall structure of the present invention; Figure 2 is a schematic diagram of the sectional structure in the present invention; Figure 3 is a schematic diagram of the mixing assembly structure in the present invention; Figure 4 is a schematic diagram of the clamping member structure in the present invention; Figure 5 is a schematic diagram of the liquid inlet assembly structure in the present invention; Figure 6 In the present invention Figure 5 is a schematic diagram of the structure of Area A in; Figure 7 is a schematic diagram of the moving assembly structure in the present invention; Figure 8 is a schematic diagram of the rotation limiting member structure in the present invention; Figure 9 is a schematic diagram of the connection structure between the driving member and the mixing assembly in the present invention.

[0018] In the figure: 1. Device housing; 2. Swab; 3. Notch; 4. Detection piece; 5. Reagent tube; 6. Rotating block; 7. Gear ring; 8. Spur gear; 9. Rotating shaft; 10. Driven wheel; 11. Belt; 12. Driving wheel; 13. Sliding sleeve; 14. Slot; 15. Clamping block; 16. Spring; 17. First groove; 18. Support piece; 19. Connecting pipe; 20. Liquid outlet hole; 21. Thimble; 22. Liquid outlet pipe; 23. Sliding frame; 24. Threaded block; 25. Reciprocating lead screw; 26. Rotating plate; 27. Ratchet gear; 28. Ratchet pawl; 29. Rotating column; 30. Torsion spring; 31. Motor; 32. Coupling; 33. Driving shaft; 34. Sliding plate; 35. Clamp; 36. Second groove; 37. Top cover. Detailed Embodiments

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0020] Embodiment 1: Please refer to Figures 1 - 8 , a new bunyavirus antigen-antibody detection kit shown in the figure, including a device housing 1, a swab 2 is inserted into the device housing 1, a top cover 37 is arranged at the top end position of the device housing 1. Through the design of the top cover 37, the swab 2 and the parts in the device can be protected. The swab 2 is inserted inside the device housing 1, which is convenient for carrying the swab 2 and can ensure the cleanliness of the swab 2 at the same time. A notch 3 is provided on the device housing 1, and a detection piece 4 is arranged in the notch 3. The position of the notch 3 is on the side of the device housing 1, which is convenient for putting the detection piece 4 into the device housing 1. At the same time, after the detection is completed, it is convenient to take out the detection piece 4 for observation. A reagent tube 5 is arranged above the detection piece 4. A sliding plate 34 is slidably connected to the device housing 1, and a clamp 35 is fixedly connected to the sliding plate 34. The clamp 35 is clamped with the reagent tube 5. The reagent tube 5 is fixed by the clamp 35, so as to facilitate pulling out the reagent tube 5 for replacement. Second grooves 36 are provided at both ends of the sliding plate 34 on the device housing 1, which is convenient for pulling out the sliding plate 34. A liquid outlet pipe 22 is installed at the bottom end position of the reagent tube 5. A liquid dripping groove and an observation groove are provided on the detection piece 4. The reagent tube 5 is installed directly above the liquid dripping groove; it further includes: a mixing component for fully mixing the reagents inside the swab 2 and the reagent tube 5, and the mixing component is installed directly above the reagent tube 5; a liquid inlet component for delivering the mixed reagent solution inside the reagent tube 5 to the detection piece 4, and the liquid inlet component is installed below the reagent tube 5; a moving component for driving the detection piece 4 to move, the moving component is cooperatively arranged with the liquid inlet component, and the moving component is installed at the bottom end position of the device housing 1.

[0021] The mixing assembly includes a rotating block 6 rotatably connected to the device housing 1, a clamping piece for clamping the swab 2 is installed on the rotating block 6, and the swab 2 is clamped by the clamping piece so that the swab 2 is stably inserted into the reagent tube 5, and the outer side of the rotating block 6 is fixedly connected to a gear ring 7 rotatably connected to the device housing 1, and the outer side of the gear ring 7 is meshed with a flat gear 8, and the axis of the flat gear 8 is fixedly connected to a rotating shaft 9 rotatably connected to the device housing 1, and the end of the rotating shaft 9 is fixedly connected to a driven wheel 10, and the outer side of the driven wheel 10 is transmission-connected to a belt 11, and the belt 11 is transmission-connected to a driving wheel 12, and the shaft of the driving wheel 12 A driving member is connected to the center position, and the driving force provided by the driving member, combined with the mechanical transmission effect, can make the rotating block 6 rotate, and the rotating block 6 drives the clamping member to rotate inside the reagent tube 5, so that the sample adsorbed on the swab 2 is evenly mixed in the reagent inside the reagent tube 5; the clamping member includes a sliding sleeve 13 plugged into the rotating block 6, and a slot 14 adapted to the sliding sleeve 13 is provided on the rotating block 6, so that the sliding sleeve 13 and the rotating block 6 are detachably connected, thereby facilitating the removal of the sliding sleeve 13, and two groups of clamping blocks 15 are symmetrically installed in the sliding sleeve 13, and the position where the clamping block 15 contacts the swab 2 An anti-skid pad is provided at the position, and the friction between the swab 2 and the clamping block 15 is increased by the setting of the anti-skid pad, so as to improve the stability of the swab 2, avoid relative sliding between the clamping block 15 and the swab 2, and affect the mixing efficiency of the swab 2 and the reagent. The two groups of clamping blocks 15 are slidably connected to the sliding sleeve 13, and the clamping block 15 is fixedly connected to the spring 16 fixedly connected to the sliding sleeve 13. The two groups of clamping blocks 15 are tightly clamped to the swab 2 by the action of the spring 16, and the end surface of the rotating block 6 is provided with a first groove 17 located on both sides of the sliding sleeve 13. The design of the first groove 17 makes it easy to move the sliding sleeve 13 from During the detection process, the swab 2 is inserted into the nasal cavity of the person to be detected and stirred to collect the sample, and then the collected sample is inserted into the reagent tube 5. Then, the driving wheel 12 drives the driven wheel 10 to rotate through the belt 11 through the driving member, and then the rotating shaft 9 drives the flat gear 8 to rotate. Therefore, the rotating block 6 is driven to rotate through the gear ring 7, and then the clamping member drives the swab 2 to rotate inside the reagent tube 5. The reagent inside the reagent tube 5 is mixed with the sample collected on the swab 2 through the rotation. Through mechanical mixing, manual intervention is avoided and the mixing efficiency is improved.

[0022] The liquid inlet assembly includes a support piece 18 installed above the detection piece 4. The support piece 18 facilitates the protection of the detection piece 4 to avoid damage. The end of the support piece 18 is fixedly connected to a connecting pipe 19. A number of groups of liquid outlet holes 20 are provided on the connecting pipe 19, and the end of the connecting pipe 19 is fixedly connected to a thimble 21. By moving the thimble 21, the thimble 21 is inserted into the reagent tube 5. After that, the mixed reagent in the reagent tube 5 will flow into the connecting pipe 19 through the thimble 21, and then be discharged from the liquid outlet holes 20 and flow to the detection piece 4. The thimble 21 is communicated with the connecting pipe 19, and the thimble 21 is inserted on the liquid outlet pipe 22; The moving assembly includes a sliding frame 23 sleeved on the outer side of the detection piece 4. The sliding frame 23 is slidably connected to the device housing 1. The detection piece 4 is placed on the sliding frame 23. By moving the sliding frame 23, the detection piece 4 squeezes the thimble 21 to move. A threaded block 24 is fixedly connected to the sliding frame 23, and a reciprocating lead screw 25 is threadedly connected to the threaded block 24. The end of the reciprocating lead screw 25 is connected to a rotation limiting part. The function of the rotation limiting part is that during the mixing of the reagent, the reciprocating lead screw 25 will not rotate. On the contrary, after the mixing is completed, the reciprocating lead screw 25 will rotate to move the sliding frame 23. The rotation limiting part is connected to the driving part. After the reagent and the sample are mixed, at this time, by controlling the driving part to reverse, during the reverse rotation of the driving part, the reciprocating lead screw 25 will rotate. By the rotation of the reciprocating lead screw 25, the threaded block 24 will drive the sliding frame 23 to move upward. By the upward movement of the sliding frame 23, the detection piece 4 will be driven to move upward. Therefore, the detection piece 4 will squeeze the thimble 21 to move upward. After the thimble 21 moves upward, the thimble 21 will be inserted into the reagent tube 5. After that, the mixed solution in the reagent tube 5 will enter the connecting pipe 19 through the thimble 21, and then flow out through the liquid outlet holes 20 into the drip trough on the detection piece 4. Therefore, the dripping process is automatically controlled mechanically, avoiding the situation that the dripping amount is difficult to control due to manual intervention.

[0023] Embodiment 2: Please refer to Figure 5 and Figure 8, This embodiment further elaborates on Embodiment 1. In the illustrated figure, the driving member includes a motor 31 fixed inside the device housing 1. The output end of the motor 31 is fixedly connected to a coupling 32. The end of the coupling 32 is fixedly connected to a driving shaft 33 fixedly connected to the driving wheel 12. The driving shaft 33 is also fixedly connected to the rotating plate 26, providing a stable driving force for the rotation of the reciprocating lead screw 25 and the rotating shaft 9. During the process of mixing the sample and the reagent, at this time, the motor 31 is controlled to rotate forward. The forward rotation of the motor 31 will drive the coupling 32 to rotate. The rotation of the coupling 32 will drive the driving shaft 33 to rotate, and then drive the driving wheel 12 to rotate. During this process, the forward rotation of the driving shaft 33 will not cause the rotation limiting member to drive the reciprocating lead screw 25 to rotate. Therefore, during the mixing process, the detection piece 4 will not move along with the sliding carriage 23. After the mixing is completed, the motor 31 is controlled to rotate in reverse. At this time, the reverse rotation of the driving shaft 33 will cause the rotation limiting member to operate and drive the reciprocating lead screw 25 to rotate. At this time, the sliding carriage 23 will move upward due to the rotation of the reciprocating lead screw 25, and then the detection piece 4 will move upward to prepare to receive the mixed solution.

[0024] Embodiment 3: Please refer to Figure 8 and Figure 9 , This embodiment further elaborates on other embodiments. In the illustrated figure, the rotation limiting member includes a rotating plate 26 connected to the driving member. The end of the rotating plate 26 is rotatably connected to a ratchet gear 27 fixedly connected to the reciprocating lead screw 25. The outside of the ratchet gear 27 is engaged with a ratchet pawl 28. The ratchet pawl 28 is fixedly connected to a rotating column 29 rotatably connected to the rotating plate 26. The rotating column 29 is fixedly connected to a torsion spring 30 fixedly connected to the rotating plate 26. During the process of mixing the swab 2 and the reagent, the ratchet pawl 28 and the ratchet gear 27 will be disengaged from each other. Therefore, the rotation of the rotating plate 26 will not drive the ratchet gear 27 to rotate. Therefore, the reciprocating lead screw 25 will not rotate in this state. On the contrary, the reciprocating lead screw 25 will rotate. When the driving shaft 33 rotates forward, it will drive the rotating plate 26 to rotate forward. The forward rotation of the rotating plate 26 will drive the ratchet pawl 28 to rotate forward. During the forward rotation of the ratchet pawl 28, it will be disengaged from the ratchet gear 27. Therefore, during this process, the rotation of the rotating plate 26 will not drive the ratchet gear 27 to rotate. On the contrary, when the driving shaft 33 drives the rotating plate 26 to rotate in reverse, at this time, the ratchet pawl 28 will engage with the ratchet gear 27 to make the ratchet gear 27 rotate, and then drive the reciprocating lead screw 25 to rotate.

[0025] Working principle: During the detection process, first pull out the sliding plate 34, pour the reagent into the reagent tube 5, then insert the sliding plate 34 back into the device housing 1 to reset it. Then place the test strip 4 on the sliding rack 23. After that, insert the swab 2 into the nasal cavity of the person to be tested and stir to collect the sample. Then remove the sliding sleeve 13, put the swab 2 into the reagent tube 5. Subsequently, insert the sliding sleeve 13 into the slot 14 again. Through the action of the two sets of clamping blocks 15, the swab 2 is clamped, so that the swab 2 is fixed between the two sets of clamping blocks 15. Then control the motor 31 to rotate forward. During this process, the forward rotation of the motor 31 will drive the coupling 32 to rotate. Through the rotation of the coupling 32, the drive shaft 33 will be driven to rotate. The drive shaft 33 drives the driving wheel 12 to rotate, and then drives the driven wheel 10 to rotate through the belt 11. Further, the rotation of the driven wheel 10 will drive the rotating shaft 9 to rotate, thus driving the spur gear 8 to rotate. Through the rotation of the spur gear 8, the gear ring 7 will be driven to rotate, thus driving the rotating block 6 fixedly connected thereto to rotate. Since the sliding sleeve 13 is inserted into the rotating block 6, the sliding sleeve 13 will be driven to rotate when the rotating block 6 rotates. Further, through the rotation of the sliding sleeve 13, the two sets of clamping blocks 15 drive the swab 2 to rotate. Therefore, the swab 2 rotates rapidly in the reagent tube 5 and is fully mixed with the reagent in the reagent tube 5, avoiding manual intervention for mixing. Through mechanical automatic control of the mixing, the mixing efficiency is improved, and the situation that the sample and the reagent are not thoroughly mixed due to manual mixing, which affects the detection result, is avoided. During this process, the forward rotation of the drive shaft 33 will cause the rotating plate 26 to rotate forward. The forward rotation of the rotating plate 26 will cause the pawl 28 to rotate forward accordingly. However, the forward rotation of the pawl 28 will disengage from the ratchet gear 27. Therefore, when the drive shaft 33 rotates forward, the ratchet gear 27 will not drive the reciprocating lead screw 25 to rotate. That is, during the mixing of the swab 2 and the reagent, the sliding rack 23 will not drive the test strip 4 to move; After the swab 2 finishes mixing with the reagent, by controlling the reverse rotation of the motor 31, the reverse rotation of the motor 31 drives the reverse rotation of the drive shaft 33. At this time, the rotating plate 26 will follow the reverse rotation of the drive shaft 33. At this time, the pawl 28 will engage with the ratchet gear 27 to drive the ratchet gear 27 to rotate, thus driving the reciprocating lead screw 25 to rotate. During the rotation of the reciprocating lead screw 25, it will drive the threaded block 24 to move. The movement of the threaded block 24 will drive the sliding frame 23 and the detection piece 4 to move. During the movement of the detection piece 4, it will gradually approach the support piece 18 until they come into contact. After contact, the sliding frame 23 continues to drive the detection piece 4 to move so that the detection piece 4 squeezes the support piece 18. Therefore, the support piece 18 drives the connecting pipe 19 and the thimble 21 to move. After the thimble 21 moves, it will insert into the reagent tube 5. Subsequently, the mixed solution in the reagent tube 5 will flow into the connecting pipe 19 along with the thimble 21, and then flow through the liquid outlet hole 20 into the drip tank on the detection piece 4. After the threaded block 24 moves to the top position of the reciprocating lead screw 25, the connecting pipe 19 will be pushed into the liquid outlet pipe 22. At this time, the liquid outlet hole 20 will be covered by the liquid outlet pipe 22, so that the reagent in the reagent tube 5 will not continue to flow out, avoiding contamination and virus transmission. And after the threaded block 24 moves to the top position of the reciprocating lead screw 25, the threaded block 24 will move in the reverse direction due to the characteristics of the reciprocating lead screw 25. After the sliding frame 23 moves to the reset position, the motor 31 stops running. After waiting for a period of time, take out the detection piece 4 to judge the detection result, and then take out the reagent tube 5 and dispose of it together with the detection piece 4. Since the device has not come into contact with the mixed reagent, it is not contaminated, and thus can continue to be used reciprocally.

[0026] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0027] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A new Bunyavirus antigen-antibody detection kit, characterized in that: include: A device housing (1), a swab (2) is inserted into the device housing (1), and a notch (3) is provided on the device housing (1), a detection sheet (4) is provided in the notch (3), a reagent tube (5) is provided above the detection sheet (4), and a liquid outlet tube (22) is installed at the bottom end of the reagent tube (5); Also includes: A mixing component, used for fully mixing the swab (2) and the reagent in the reagent tube (5), the mixing component being installed directly above the reagent tube (5); A liquid inlet assembly, used for conveying the mixed reagent solution in the reagent tube (5) to the detection sheet (4), the liquid inlet assembly being installed at a position below the reagent tube (5); A moving component is used to drive the detection sheet (4) to move, the moving component and the liquid inlet component are arranged in cooperation with each other, and the moving component is installed at the bottom end of the device housing (1).

2. A novel bunyavirus antigen-antibody detection kit according to claim 1, characterized in that: The mixing assembly comprises a rotating block (6) rotatably connected to the device housing (1), a clamping member for clamping the swab (2) being mounted on the rotating block (6), and a gear ring (7) rotatably connected to the device housing (1) being fixedly connected to the outer side surface of the rotating block (6), a flat gear (8) being meshed and driven on the outer side surface of the gear ring (7), a rotating shaft (9) rotatably connected to the device housing (1) being fixedly connected to the axis of the flat gear (8), a driven wheel (10) being fixedly connected to the end of the rotating shaft (9), a belt (11) being transmission-connected to the outer side surface of the driven wheel (10), a driving wheel (12) being transmission-connected to the belt (11), and a driving member being connected to the axis of the driving wheel (12).

3. A new bunyavirus antigen-antibody detection kit according to claim 2, characterized in that: The clamping member comprises a sliding sleeve (13) plugged into the rotating block (6); a slot (14) adapted to the sliding sleeve (13) is provided on the rotating block (6); two groups of clamping blocks (15) are symmetrically installed in the sliding sleeve (13); both groups of clamping blocks (15) are slidably connected to the sliding sleeve (13); a spring (16) fixedly connected to the sliding sleeve (13) is fixedly connected to the clamping block (15); and a first groove (17) located on both sides of the sliding sleeve (13) is provided on an end surface of the rotating block (6).

4. A novel bunyavirus antigen-antibody detection kit according to claim 1, characterized in that: The liquid inlet assembly comprises a support sheet (18) mounted above the detection sheet (4); the end of the support sheet (18) is fixedly connected to a connecting tube (19); the connecting tube (19) is provided with a plurality of groups of liquid outlet holes (20); and the end of the connecting tube (19) is fixedly connected to a ejector pin (21); the ejector pin (21) is connected to the connecting tube (19) and is plugged into the liquid outlet tube (22).

5. A new bunyavirus antigen-antibody detection kit according to claim 2, characterized in that: The moving assembly comprises a sliding frame (23) sleeved on the outer side of the detection sheet (4), the sliding frame (23) being slidably connected to the device housing (1), and a threaded block (24) being fixedly connected to the sliding frame (23), a reciprocating screw rod (25) being threadedly connected to the threaded block (24), an end of the reciprocating screw rod (25) being connected to a limited rotation member, and the limited rotation member being connected to the driving member.

6. A novel bunyavirus antigen-antibody detection kit according to claim 5, characterized in that: The rotation limiting member comprises a rotating plate (26) connected to the driving member, the end of the rotating plate (26) is rotatably connected to a ratchet gear (27) fixedly connected to the reciprocating screw rod (25), a pawl (28) is meshed on the outer side of the ratchet gear (27), a rotating column (29) rotatably connected to the rotating plate (26) is fixedly connected to the pawl (28), and a torsion spring (30) fixedly connected to the rotating plate (26) is fixedly connected to the rotating column (29).

7. A novel bunyavirus antigen-antibody detection kit according to claim 6, characterized in that: The driving member comprises a motor (31) fixed inside the device housing (1); an output end of the motor (31) is fixedly connected to a coupling (32); an end of the coupling (32) is fixedly connected to a driving shaft (33) fixedly connected to the driving wheel (12); and the driving shaft (33) is also fixedly connected to the rotating plate (26).

8. A novel bunyavirus antigen-antibody detection kit according to claim 1, characterized in that: A sliding plate (34) is slidably connected to the device housing (1), a clamp (35) is fixedly connected to the sliding plate (34), the clamp (35) is clamped with the reagent tube (5), and second grooves (36) are provided on the device housing (1) at both ends of the sliding plate (34).

9. A novel bunyavirus antigen-antibody detection kit according to claim 3, characterized in that: An anti-slip pad is provided at the position where the clamping block (15) contacts the swab (2).

10. A novel bunyavirus antigen-antibody detection kit according to claim 1, characterized in that: A top cover (37) is provided at the top end of the device housing (1).

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