Pressure-amplified column filling device
By designing a pressure amplification column filling device with unequal diameter pistons, the problem of insufficient filling power in the prior art will be solved, and more efficient column filling and performance improvements are achieved.
Patent Information
- Application Number
- CN202510129786.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-05
- Publication Date
- 2025-05-30
AI Technical Summary
The existing chromatographic column filling devices cannot effectively amplify the column filling pressure, which makes it difficult to obtain sufficient column filling power during the filling process, affecting the performance of the column.
A pressure amplification column filling device including pistons at both ends is designed to amplify the pressure of the external fluid by varying areas at both ends of the piston, thereby providing greater column filling power.
By amplifying the pressure, more efficient column filling is achieved, which can easily and at low cost to obtain higher column filling power, improving the performance of the column.
Smart Images
Figure CN120054037A_ABST
Abstract
Description
Technical Field
[0001] This patent relates to the filling of chromatographic columns. Background Art
[0002] The chromatographic column is a core component in chromatographic analysis, and the sample is separated in the chromatographic column. The chromatographic packed column is prepared by packing the filler in the empty tube of the chromatographic column. The preparation of a high-performance chromatographic packed column is closely related to the selection of the filler and the column packing technique. When the filler is determined, whether a uniform and dense column bed can be filled is the key to obtaining a high-performance column. Generally, the filling of a chromatographic column requires a pressurizing device, which on the one hand provides power to push the filler into the chromatographic column, and on the other hand needs to provide power to compact the chromatographic filler. In order to obtain the pressure required for column filling more conveniently, the present invention designs a column filling device with pressure amplification, which can further amplify the pressure of the external fluid and then use it for the filling of the chromatographic column.
[0003] This pressure amplification device is realized by pistons with unequal diameters at both ends. According to P1*S1 = P2*S2, the input pressure can be amplified according to the area ratio of the two ends of the piston to obtain a larger output pressure for the filling of the chromatographic column.
[0004] Generally, the filling power of a traditional column filling device is directly connected to the chromatographic column filling device. In the patent CN118671250A, a piston pump is directly used for the filling of the chromatographic column, and this scheme cannot further amplify the filling pressure generated. This device can amplify the pressure of the external fluid with a piston of unequal diameters and then use it for the filling of the chromatographic column, and can obtain higher filling power more easily and at lower cost. Summary of the Invention
[0005] The present invention designs a column filling device with pressure amplification, including pistons with unequal diameters at both ends and the matching piston cavities, packing cavities, and chromatographic column connection interfaces. The areas of the two ends of the piston are not equal. The large-area end of the piston is connected to the input end of the external fluid, and this fluid can be a gas or a liquid. The small-area end is in contact with the column filling liquid, and the amplified pressure can be used for the filling of the chromatographic column.
[0006] The packing cavity can be integrally designed and directly connected to the small-area end of the piston cavity, or can be connected to it through a connecting pipeline.
[0007] During column filling, the external fluid pushes the large-area end of the piston to generate a thrust, which drives the small-area end of the piston to compress the liquid (the liquid itself is almost incompressible), and the amplified liquid pressure can be calculated according to the area ratio. The liquid after power amplification pushes the slurry-state filler into the chromatographic column to achieve the filling of the chromatographic column. Description of the Drawings
[0008] Figure 1Structural schematic diagram of a column packing device with pressure amplification provided by the present invention. Detailed implementation manners
[0009] The device of the present invention will be further described below with reference to the accompanying drawings.
[0010] As Figure 1 shown, it is a structural schematic diagram of a column packing device with pressure amplification provided by the present invention, including a chromatographic column 1, a chromatographic column interface 2, a packing cavity 3, piston cavities 4 and 6, and a piston 5. The interface is used to fix the chromatographic column, the piston cavity is used to accommodate the piston, and the packing cavity is used to store the slurry packing. When filling the chromatographic column, first place the packing in the packing cavity, and fill the packing cavity and the piston cavity 4 with the column packing liquid. Then, apply pressure to the large-area end of the piston through an external fluid to drive the piston to move, thereby driving the small-area end of the piston to push the liquid in the piston cavity 4, and pushing the packing into the chromatographic column to achieve the packing of the chromatographic column.
[0011] The present invention is not limited to the above embodiments. All embodiments obtained without any creative work fall within the protection scope of the present invention.
Claims
1. A pressure-amplified column filling device, characterized in that: The fluid is used to provide the pressure required for filling the column, and the pressure is amplified by a piston with different areas at both ends for filling the chromatographic column. It mainly includes an unequal diameter piston, a piston cavity, a filler cavity and an interface connected to the chromatographic column.
2. The device according to claim 1, characterized in that There are two end faces of different areas at both ends of the piston; the large-area end of the piston contacts the external fluid through the pipeline, and the external fluid pushes the piston to move, thereby driving the small-area end to push the liquid at this end, pushing the filler in the filler cavity into the chromatographic column.
3. The device according to claim 1, characterized in that The liquid at the small area end of the piston can be connected to the packing cavity directly or indirectly through a pipeline; the packing cavity can be used in conjunction with a stirring device to keep the packing in a homogenous state.
4. The device according to claim 1, characterized in that The piston cavity and the filler cavity may be made of hard materials such as ceramics and stainless steel; the piston may be made of hard materials such as ceramics and stainless steel; the piston may be a whole or assembled from separate parts.
5. The device according to claim 1, characterized in that Both ends of the piston can be sealed with the piston cavity through a sealing ring or a combination of multiple sealing rings to ensure that there is no leakage.
6. The device according to claim 1, characterized in that An external pressure gauge can be connected to monitor the pressure of the column.
7. The device according to claim 1, characterized in that The packing cavity is connected with the piston cavity and can be separated by a sieve plate, so that the liquid can pass through but the packing cannot pass through.
8. The device according to claim 1, characterized in that After the external fluid pressure is released, the piston can return to its original position through a spring or a screw device.