Preparation method of antibacterial hemostatic gauze

Abstract

The invention discloses a preparation method of antibacterial hemostatic gauze, and belongs to the technical field of gauze preparation, the preparation method comprises pretreatment, first soaking treatment, second soaking treatment, third soaking treatment, fourth soaking treatment, fifth soaking treatment, sixth soaking treatment and post-treatment; the primary soaking treatment comprises the following steps: completely soaking the pretreated gauze in 1, 5-pentanediol, standing at room temperature, taking out the gauze, and vertically hanging to obtain the gauze subjected to the primary soaking treatment; the fourth soaking treatment comprises the following steps: completely soaking the gauze subjected to the third soaking treatment in a zinc oxide dispersion liquid, standing at room temperature, taking out the gauze, and vertically hanging to obtain the gauze subjected to the fourth soaking treatment; the gauze prepared by the preparation method disclosed by the invention is good in lasting antibacterial function, hemostatic function, operability, storage resistance and air permeability, and the indexes of the lasting antibacterial function of the surface are uniformly distributed.

Description

Technical Field

[0001] The present invention relates to the technical field of gauze preparation, and particularly relates to a preparation method of an antibacterial hemostatic gauze. Background Art

[0002] Gauze is a cotton fabric with sparse warp and weft, belonging to textile raw materials, and has wide applications in the medical field and the life field, such as wound dressing, hemostasis, skin protection, and skin cleaning. Classified by raw material components, gauze is mainly divided into pure cotton gauze, purified fiber gauze, and blended gauze. Among them, the raw material component of pure cotton gauze is 100% cotton, which has excellent air permeability, softness, skin-friendliness, and water absorbency, and is widely used in the medical field.

[0003] When applying pure cotton gauze to the medical field, it is necessary to perform a sterilization treatment on the pure cotton gauze. Commonly used sterilization treatment methods include boiling treatment, ultraviolet irradiation treatment, alcohol treatment, chemical treatment, etc. However, the pure cotton gauze treated by the above methods all has the problem of non-persistent antibacterial property. Moreover, with the continuous progress of medical technology and textile technology, the requirements for gauze are getting higher and higher. Although the existing pure cotton gauze has good air permeability, softness, skin-friendliness, and water absorbency, it does not have a hemostatic function and a persistent antibacterial function. In use, additional hemostatic drugs and antibacterial drugs need to be used. The excessive use of drugs will affect the air permeability of the wound. Therefore, it is necessary to develop a gauze with a persistent antibacterial function and a hemostatic function.

[0004] Through market research, it is found that the existing gauze with a persistent antibacterial function and a hemostatic function is mainly prepared by treating the gauze with a treatment solution. The treatment includes chemical treatment and physical treatment. Among them, the chemical treatment is to react the active ingredient in the chemical treatment solution with the hydroxyl groups on the surface of the pure cotton gauze, and use the method of chemical grafting to introduce functional groups with a persistent antibacterial function and a hemostatic function onto the surface of the gauze; the physical treatment is to interact the active ingredient in the physical treatment solution with the hydroxyl groups on the surface of the pure cotton gauze, and use physical adsorption to fix the components with a persistent antibacterial function and a hemostatic function on the surface of the gauze. However, through specific experiments, it is found that the chemical treatment method is complex and requires professional heating equipment and stirring equipment. Some chemical treatments also require centrifugal equipment and the use of protective gases for protection, with poor operability; the physical treatment method is simple and only requires simple stirring and soaking. However, the binding force between the components with a persistent antibacterial function and a hemostatic function and the gauze is poor, resulting in poor storage resistance of the prepared antibacterial hemostatic gauze. It is easy to fall off and become ineffective during long-term storage, and the binding amount is small and the air permeability is poor. Therefore, it will also cause the persistent antibacterial function, hemostatic function, and air permeability of the prepared antibacterial hemostatic gauze to deteriorate.

[0005] In view of the above problems, the inventor improved the physical treatment solution, specifically by adding an adsorbent material, such as hydroxyapatite or graphene oxide, to the physical treatment solution. The adsorbent material can promote the binding between the components with persistent antibacterial and hemostatic functions and the gauze, and can also increase the binding amount of the components with persistent antibacterial and hemostatic functions on the surface of the gauze, thereby improving the persistent antibacterial and hemostatic functions of the prepared antibacterial hemostatic gauze. However, the dispersibility of the adsorbent material is poor, and it is easy to have uneven dispersion on the surface of the gauze, resulting in uneven distribution of the indexes of the persistent antibacterial function on the surface of the prepared antibacterial hemostatic gauze. Summary of the Invention

[0006] In view of the deficiencies of the prior art, the present invention provides a preparation method of an antibacterial hemostatic gauze. The prepared gauze has good persistent antibacterial function, hemostatic function, operability, storage resistance, and air permeability, and the indexes of the persistent antibacterial function on the surface are evenly distributed.

[0007] To solve the above technical problems, the technical solutions adopted by the present invention are as follows: A preparation method of an antibacterial hemostatic gauze, including: pretreatment, first immersion treatment, second immersion treatment, third immersion treatment, fourth immersion treatment, fifth immersion treatment, sixth immersion treatment, and post-treatment; In the pretreatment, the degreased pure cotton gauze is washed with water and then dried to obtain the pretreated gauze; In the pretreatment, the drying temperature is 115 - 125 °C and the time is 1.5 - 2 h; In the first immersion treatment, the pretreated gauze is completely immersed in 1,5 - pentanediol, left standing at room temperature for 40 - 50 min, the gauze is taken out and vertically hung for 40 - 50 min to obtain the gauze after the first immersion treatment; In the first immersion treatment, the mass - to - volume ratio of the pretreated gauze to 1,5 - pentanediol is 500 g: 3000 - 3200 mL; In the second immersion treatment, the gauze after the first immersion treatment is completely immersed in an aqueous zinc chloride solution, left standing at room temperature for 40 - 50 min, the gauze is taken out and vertically hung for 20 - 30 min to obtain the gauze after the second immersion treatment; In the second immersion treatment, the mass fraction of the aqueous zinc chloride solution is 10%; In the first immersion treatment, the mass - to - volume ratio of the pretreated gauze to the aqueous zinc chloride solution in the second immersion treatment is 500 g: 8500 - 9500 mL; In the third immersion treatment, the gauze after the second immersion treatment is completely immersed in a tannic acid aqueous solution, left standing at room temperature for 40 - 50 min, the gauze is taken out and vertically hung for 20 - 30 min to obtain the gauze after the third immersion treatment; In the three - time soaking treatment, the mass fraction of the tannic acid aqueous solution is 5%; In the one - time soaking treatment, the mass - to - volume ratio of the pretreated gauze to the tannic acid aqueous solution in the three - time soaking treatment is 500 g: 8500 - 9500 mL; In the four - time soaking treatment, the gauze after the three - time soaking treatment is completely immersed in the zinc oxide dispersion liquid, left standing at room temperature for 60 - 70 min, the gauze is taken out, and vertically hung for 30 - 40 min to obtain the gauze after the four - time soaking treatment; The preparation method of the zinc oxide dispersion liquid is as follows: zinc oxide is added to purified water, stirred at room temperature for 50 - 60 min, α - lactalbumin is added, and stirring is continued for 30 - 40 min, then 1,5 - pentanediol is added, and stirring is continued for 20 - 30 min to obtain the zinc oxide dispersion liquid; In the preparation of the zinc oxide dispersion liquid, the particle size of the zinc oxide is 50 nm; The dosage ratio of zinc oxide, purified water, α - lactalbumin, and 1,5 - pentanediol is 48 - 50 g: 8320 - 9300 mL: 3 - 3.2 g: 180 - 200 mL; In the one - time soaking treatment, the mass - to - volume ratio of the pretreated gauze to the zinc oxide dispersion liquid in the four - time soaking treatment is 500 g: 8500 - 9500 mL; In the five - time soaking treatment, the gauze after the four - time soaking treatment is completely immersed in the carboxymethyl chitosan aqueous solution, left standing at room temperature for 40 - 50 min, the gauze is taken out, and vertically hung for 30 - 40 min to obtain the gauze after the five - time soaking treatment; In the five - time soaking treatment, the mass fraction of the carboxymethyl chitosan aqueous solution is 2%; In the carboxymethyl chitosan aqueous solution, the molecular weight of carboxymethyl chitosan is 30 kDa, the degree of deacetylation is 90%, and the degree of substitution is 0.75; In the one - time soaking treatment, the mass - to - volume ratio of the pretreated gauze to the carboxymethyl chitosan aqueous solution in the five - time soaking treatment is 500 g: 8500 - 9500 mL; In the six - time soaking treatment, the gauze after the five - time soaking treatment is completely immersed in the calcium chloride aqueous solution, left standing at room temperature for 40 - 50 min, the gauze is taken out, and vertically hung for 30 - 40 min to obtain the gauze after the six - time soaking treatment; In the six - time soaking treatment, the mass fraction of the calcium chloride aqueous solution is 5%; In the one - time soaking treatment, the mass - to - volume ratio of the pretreated gauze to the calcium chloride aqueous solution in the six - time soaking treatment is 500 g: 8500 - 9500 mL; The post-treatment is to irradiate the gauze after six times of immersion treatment under an ultraviolet lamp, let it stand at room temperature for 20 - 30 min, and then dry it to obtain the antibacterial hemostatic gauze; In the post-treatment, the power of the ultraviolet lamp is 300 W and the wavelength is 365 nm; The drying temperature is 115 - 125 °C and the time is 2.5 - 3 h; The gauze after six times of immersion treatment is placed at a position 80 - 90 cm away from the ultraviolet lamp.

[0008] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The preparation method of the antibacterial hemostatic gauze of the present invention includes pre-treatment, first immersion treatment, second immersion treatment, third immersion treatment, fourth immersion treatment, fifth immersion treatment, sixth immersion treatment, and post-treatment. Among them, the pre-treatment is to wash and dry the degreased pure cotton gauze. The first immersion treatment is to treat the pre-treated gauze with 1,5-pentanediol. 1,5-Pentanediol can treat the surface of the gauze, improve the wettability of the gauze surface, and is more conducive to the later combination with tannic acid and the promotion of the dispersion of tannic acid and zinc oxide. The second immersion treatment is to treat the gauze with an aqueous solution of zinc chloride. Zinc ions can be adsorbed on the surface of the gauze and 1,5-pentanediol, playing a role in persistent antibacterial. The third immersion treatment is to treat the gauze with an aqueous solution of tannic acid. Tannic acid crosslinks with the hydroxyl groups and zinc ions on the surface of the gauze to form a crosslinked substance on the surface of the gauze. The fourth immersion treatment is to treat the gauze with a zinc oxide dispersion. When preparing the zinc oxide dispersion, first disperse zinc oxide in pure water, then add α-lactalbumin. α-Lactalbumin can be adsorbed on the surface of zinc oxide to improve the dispersion of zinc oxide, and then add 1,5-pentanediol. 1,5-Pentanediol can be adsorbed on the outer layer of zinc oxide to further improve the dispersion of zinc oxide. During the treatment process, zinc oxide in the zinc oxide dispersion can be combined with the surface of the gauze through 1,5-pentanediol on the surface, and 1,5-pentanediol can crosslink with tannic acid through hydroxyl groups, thereby improving the binding force with the gauze. α-Lactalbumin can play a role in promoting synergistic antibacterial. The fifth immersion treatment is to treat the gauze with an aqueous solution of carboxymethyl chitosan. Carboxymethyl chitosan binds to the surface of zinc oxide through the interaction with 1,5-pentanediol, playing an antibacterial and hemostatic role. The sixth immersion treatment is to treat the gauze with an aqueous solution of calcium chloride. Calcium ions promote the crosslinking of carboxymethyl chitosan, and 1,5-pentanediol on the surface of zinc oxide can achieve the purpose of secondary crosslinking, improving the binding force of the tannic acid crosslinked layer, zinc oxide layer, and carboxymethyl chitosan layer; (2) For the preparation method of the antibacterial hemostatic gauze of the present invention, the prepared gauze has a strong persistent antibacterial function. The inhibition rate of Staphylococcus aureus is 98.6 - 99.2%, the inhibition rate of Escherichia coli is 98.5 - 99.1%, and the inhibition rate of Candida albicans is 97.8 - 98.8%; (3) The preparation method of the antibacterial hemostatic gauze of the present invention has a strong hemostatic function for the prepared gauze, and the whole blood coagulation index (BCI) is 15.54 - 16.58; (4) The preparation method of the antibacterial hemostatic gauze of the present invention has good operability, is simple, does not require professional heating equipment and stirring equipment, nor centrifugation equipment and does not use protective gas for protection; (5) The preparation method of the antibacterial hemostatic gauze of the present invention has good storage resistance for the prepared gauze. After storing the antibacterial hemostatic gauze prepared by the present invention in a light - proof environment at a temperature of 23°C and a relative humidity of 60% for 30 days, the inhibition rate decline rate of Staphylococcus aureus is 0.13 - 0.21%, the inhibition rate decline rate of Escherichia coli is 0.07 - 0.14%, and the inhibition rate decline rate of Candida albicans is 0.06 - 0.15%; (6) The preparation method of the antibacterial hemostatic gauze of the present invention has good air permeability for the prepared gauze. After stacking 10 layers of the antibacterial hemostatic gauze prepared by the present invention and fixing them, the water vapor transmission rate is 1909.44 - 1984.52 g / (m 2 •d); (7) The preparation method of the antibacterial hemostatic gauze of the present invention has a uniform distribution of the indexes of the persistent antibacterial function on the surface of the prepared gauze. Taking 10 samples on the gauze prepared by the present invention, with an interval of 10 cm between each sample, the difference between the maximum Staphylococcus aureus inhibition rate and the minimum Staphylococcus aureus inhibition rate is 0.17 - 0.31%, the difference between the maximum Escherichia coli inhibition rate and the minimum Escherichia coli inhibition rate is 0.14 - 0.25%, and the difference between the maximum Candida albicans inhibition rate and the minimum Candida albicans inhibition rate is 0.26 - 0.33%. Detailed implementation mode

[0009] In order to have a clearer understanding of the technical features, objectives, and effects of the present invention, the specific implementation mode of the present invention is now described.

[0010] Example 1 A preparation method of an antibacterial hemostatic gauze is specifically as follows: 1. Pretreatment: After washing the degreased pure cotton gauze with water, it is dried at 115°C for 1.5 h to obtain the pretreated gauze; 2. Primary immersion treatment: The pretreated gauze is completely immersed in 1,5 - pentanediol, left standing at room temperature for 40 min, and then the gauze is taken out and vertically suspended for 40 min to obtain the gauze after primary immersion treatment; The mass - to - volume ratio of the pretreated gauze to 1,5 - pentanediol is 500 g:3000 mL; 3. Secondary immersion treatment: Immerse the gauze after the primary immersion treatment completely in an aqueous zinc chloride solution, let it stand at room temperature for 40 min, take out the gauze, and hang it vertically for 20 min to obtain the gauze after the secondary immersion treatment; The mass fraction of the aqueous zinc chloride solution is 10%; The mass-to-volume ratio of the pretreated gauze in the primary immersion treatment in Step 2 to the aqueous zinc chloride solution in the secondary immersion treatment in Step 3 is 500 g:8500 mL; 4. Tertiary immersion treatment: Immerse the gauze after the secondary immersion treatment completely in an aqueous tannic acid solution, let it stand at room temperature for 40 min, take out the gauze, and hang it vertically for 20 min to obtain the gauze after the tertiary immersion treatment; The mass fraction of the aqueous tannic acid solution is 5%; The mass-to-volume ratio of the pretreated gauze in the primary immersion treatment in Step 2 to the aqueous tannic acid solution in the tertiary immersion treatment in Step 4 is 500 g:8500 mL; 5. Quaternary immersion treatment: Immerse the gauze after the tertiary immersion treatment completely in a zinc oxide dispersion, let it stand at room temperature for 60 min, take out the gauze, and hang it vertically for 30 min to obtain the gauze after the quaternary immersion treatment; The preparation method of the zinc oxide dispersion is as follows: Add zinc oxide to purified water, stir at room temperature for 50 min, add α-lactalbumin, continue stirring for 30 min, add 1,5-pentanediol, and continue stirring for 20 min to obtain the zinc oxide dispersion; The particle size of the zinc oxide is 50 nm; The dosage ratio of zinc oxide, purified water, α-lactalbumin, and 1,5-pentanediol is 48 g:8320 mL:3 g:180 mL; The mass-to-volume ratio of the pretreated gauze in the primary immersion treatment in Step 2 to the zinc oxide dispersion in the quaternary immersion treatment in Step 5 is 500 g:8500 mL; 6. Quinary immersion treatment: Immerse the gauze after the quaternary immersion treatment completely in an aqueous carboxymethyl chitosan solution, let it stand at room temperature for 40 min, take out the gauze, and hang it vertically for 30 min to obtain the gauze after the quinary immersion treatment; The mass fraction of the aqueous carboxymethyl chitosan solution is 2%; The molecular weight of carboxymethyl chitosan in the aqueous carboxymethyl chitosan solution is 30 kDa, the degree of deacetylation is 90%, and the degree of substitution is 0.75; The mass-to-volume ratio of the pretreated gauze in the primary immersion treatment in Step 2 to the aqueous carboxymethyl chitosan solution in the quinary immersion treatment in Step 6 is 500 g:8500 mL; 7. Sixth immersion treatment: Immerse the gauze after the fifth immersion treatment completely in an aqueous calcium chloride solution, let it stand at room temperature for 40 min, take out the gauze, and hang it vertically for 30 min to obtain the gauze after the sixth immersion treatment; The mass fraction of the aqueous calcium chloride solution is 5%; The mass-to-volume ratio of the pretreated gauze in the first immersion treatment in Step 2 to the aqueous calcium chloride solution in the sixth immersion treatment in Step 7 is 500 g:8500 mL; 8. Post-treatment: irradiate the gauze after the sixth immersion treatment at a distance of 80 cm under an ultraviolet lamp, let it stand at room temperature for 20 min, and then dry it at 115 °C for 2.5 h to obtain the antibacterial hemostatic gauze; The power of the ultraviolet lamp is 300 W and the wavelength is 365 nm.

[0011] Example 2 A preparation method of an antibacterial hemostatic gauze, specifically: 1. Pretreatment: Wash the degreased pure cotton gauze and then dry it at 120 °C for 2 h to obtain the pretreated gauze; 2. First immersion treatment: Immerse the pretreated gauze completely in 1,5-pentanediol, let it stand at room temperature for 45 min, take out the gauze, and hang it vertically for 45 min to obtain the gauze after the first immersion treatment; The mass-to-volume ratio of the pretreated gauze to 1,5-pentanediol is 500 g:3100 mL; 3. Second immersion treatment: Immerse the gauze after the first immersion treatment completely in an aqueous zinc chloride solution, let it stand at room temperature for 45 min, take out the gauze, and hang it vertically for 25 min to obtain the gauze after the second immersion treatment; The mass fraction of the aqueous zinc chloride solution is 10%; The mass-to-volume ratio of the pretreated gauze in the first immersion treatment in Step 2 to the aqueous zinc chloride solution in the second immersion treatment in Step 3 is 500 g:9000 mL; 4. Third immersion treatment: Immerse the gauze after the second immersion treatment completely in an aqueous tannic acid solution, let it stand at room temperature for 45 min, take out the gauze, and hang it vertically for 25 min to obtain the gauze after the third immersion treatment; The mass fraction of the aqueous tannic acid solution is 5%; The mass-to-volume ratio of the pretreated gauze in the first immersion treatment in Step 2 to the aqueous tannic acid solution in the third immersion treatment in Step 4 is 500 g:9000 mL; 5. Fourth immersion treatment: Immerse the gauze after the third immersion treatment completely in a zinc oxide dispersion, let it stand at room temperature for 65 min, take out the gauze, and hang it vertically for 35 min to obtain the gauze after the fourth immersion treatment; The preparation method of the zinc oxide dispersion is as follows: Add zinc oxide to purified water, stir at room temperature for 55 min, add α-lactalbumin, continue stirring for 35 min, add 1,5-pentanediol, and continue stirring for 25 min to obtain the zinc oxide dispersion; The particle size of the zinc oxide is 50 nm; The dosage ratio of zinc oxide, purified water, α-lactalbumin, and 1,5-pentanediol is 49 g: 9000 mL: 3.1 g: 190 mL; In the first immersion treatment in step 2, the mass-volume ratio of the pretreated gauze to the zinc oxide dispersion in the fourth immersion treatment in step 5 is 500 g: 9190 mL; 6. Fifth immersion treatment: Completely immerse the gauze after the fourth immersion treatment in an aqueous carboxymethyl chitosan solution, let it stand at room temperature for 45 min, take out the gauze, and hang it vertically for 35 min to obtain the gauze after the fifth immersion treatment; The mass fraction of the aqueous carboxymethyl chitosan solution is 2%; In the aqueous carboxymethyl chitosan solution, the molecular weight of carboxymethyl chitosan is 30 kDa, the degree of deacetylation is 90%, and the degree of substitution is 0.75; In the first immersion treatment in step 2, the mass-volume ratio of the pretreated gauze to the aqueous carboxymethyl chitosan solution in the sixth immersion treatment in step 6 is 500 g: 9000 mL; 7. Sixth immersion treatment: Completely immerse the gauze after the fifth immersion treatment in an aqueous calcium chloride solution, let it stand at room temperature for 45 min, take out the gauze, and hang it vertically for 35 min to obtain the gauze after the sixth immersion treatment; The mass fraction of the aqueous calcium chloride solution is 5%; In the first immersion treatment in step 2, the mass-volume ratio of the pretreated gauze to the aqueous calcium chloride solution in the seventh immersion treatment in step 7 is 500 g: 9000 mL; 8. Post-treatment: Irradiate the gauze after the sixth immersion treatment at a distance of 85 cm under an ultraviolet lamp, let it stand at room temperature for 25 min, and then dry it at 120 °C for 3 h to obtain the antibacterial hemostatic gauze; The power of the ultraviolet lamp is 300 W and the wavelength is 365 nm.

[0012] Example 3 A preparation method of an antibacterial hemostatic gauze is specifically as follows: 1. Pretreatment: Wash the degreased pure cotton gauze and dry it at 125 °C for 2 h to obtain the pretreated gauze; 2. First immersion treatment: Completely immerse the pretreated gauze in 1,5-pentanediol, let it stand at room temperature for 50 min, take out the gauze, and hang it vertically for 50 min to obtain the gauze after the first immersion treatment; The mass-volume ratio of the pretreated gauze to 1,5-pentanediol is 500 g: 3200 mL; 3. Secondary immersion treatment: Immerse the gauze after the primary immersion treatment completely in an aqueous zinc chloride solution, let it stand at room temperature for 50 min, take out the gauze, and hang it vertically for 30 min to obtain the gauze after the secondary immersion treatment; The mass fraction of the aqueous zinc chloride solution is 10%; The mass-volume ratio of the pretreated gauze in the primary immersion treatment in step 2 to the aqueous zinc chloride solution in the secondary immersion treatment in step 3 is 500 g: 9500 mL; 4. Tertiary immersion treatment: Immerse the gauze after the secondary immersion treatment completely in an aqueous tannic acid solution, let it stand at room temperature for 50 min, take out the gauze, and hang it vertically for 30 min to obtain the gauze after the tertiary immersion treatment; The mass fraction of the aqueous tannic acid solution is 5%; The mass-volume ratio of the pretreated gauze in the primary immersion treatment in step 2 to the aqueous tannic acid solution in the tertiary immersion treatment in step 4 is 500 g: 9500 mL; 5. Quaternary immersion treatment: Immerse the gauze after the tertiary immersion treatment completely in a zinc oxide dispersion, let it stand at room temperature for 70 min, take out the gauze, and hang it vertically for 40 min to obtain the gauze after the quaternary immersion treatment; The preparation method of the zinc oxide dispersion is as follows: Add zinc oxide into purified water, stir at room temperature for 60 min, add α-lactalbumin, continue to stir for 40 min, add 1,5-pentanediol, and continue to stir for 30 min to obtain the zinc oxide dispersion; The particle size of the zinc oxide is 50 nm; The dosage ratio of zinc oxide, purified water, α-lactalbumin, and 1,5-pentanediol is 50 g: 9300 mL: 3.2 g: 200 mL; The mass-volume ratio of the pretreated gauze in the primary immersion treatment in step 2 to the zinc oxide dispersion in the quaternary immersion treatment in step 5 is 500 g: 9500 mL; 6. Quinary immersion treatment: Immerse the gauze after the quaternary immersion treatment completely in an aqueous carboxymethyl chitosan solution, let it stand at room temperature for 50 min, take out the gauze, and hang it vertically for 40 min to obtain the gauze after the quinary immersion treatment; The mass fraction of the aqueous carboxymethyl chitosan solution is 2%; The molecular weight of carboxymethyl chitosan in the aqueous carboxymethyl chitosan solution is 30 kDa, the degree of deacetylation is 90%, and the degree of substitution is 0.75; The mass-volume ratio of the pretreated gauze in the primary immersion treatment in step 2 to the aqueous carboxymethyl chitosan solution in the quinary immersion treatment in step 6 is 500 g: 9500 mL; 7. Sixth immersion treatment: Immerse the gauze after the fifth immersion treatment completely in the calcium chloride aqueous solution, let it stand at room temperature for 50 min, take out the gauze, and hang it vertically for 40 min to obtain the gauze after the sixth immersion treatment; The mass fraction of the calcium chloride aqueous solution is 5%; The mass-to-volume ratio of the pretreated gauze in the first immersion treatment in Step 2 to the calcium chloride aqueous solution in the sixth immersion treatment in Step 7 is 500 g: 9500 mL; 8. Post-treatment: Irradiate the gauze after the sixth immersion treatment at a distance of 90 cm under an ultraviolet lamp, let it stand at room temperature for 30 min, and then dry it at 125 °C for 3 h to obtain the antibacterial hemostatic gauze; The power of the ultraviolet lamp is 300 W, and the wavelength is 365 nm.

[0013] Comparative Example 1 On the basis of the preparation method of the antibacterial hemostatic gauze in Example 2, omit the first immersion treatment step in Step 2 and the second immersion treatment step in Step 3, that is, directly perform the third immersion treatment in Step 4 on the pretreated gauze obtained in the first pretreatment step.

[0014] Comparative Example 2 On the basis of the preparation method of the antibacterial hemostatic gauze in Example 2, omit the first immersion treatment step in Step 2, that is, directly perform the second immersion treatment in Step 3 on the pretreated gauze obtained in the first pretreatment step; and in the preparation of the zinc oxide dispersion liquid in the fourth immersion treatment step in Step 5, omit the addition of 1,5-pentanediol. Specifically, change the preparation method of the zinc oxide dispersion liquid to: Add zinc oxide to purified water, stir at room temperature for 55 min, add α-lactalbumin, and continue to stir for 35 min to obtain the zinc oxide dispersion liquid; The particle size of the zinc oxide is 50 nm; The dosage ratio of zinc oxide, purified water, and α-lactalbumin is 49 g: 9000 mL: 3.1 g.

[0015] Comparative Example 3 On the basis of the preparation method of the antibacterial hemostatic gauze in Example 2, in the preparation of the zinc oxide dispersion liquid in the fourth immersion treatment step in Step 5, omit the addition of α-lactalbumin and 1,5-pentanediol. Specifically, change the preparation method of the zinc oxide dispersion liquid to: Add zinc oxide to purified water, stir at room temperature for 55 min to obtain the zinc oxide dispersion liquid; The particle size of the zinc oxide is 50 nm; The dosage ratio of zinc oxide and purified water is 49 g: 9000 mL.

[0016] Performance Test 1 According to the national standard GB / T 20944.3-2008 "Evaluation of antibacterial properties of textiles - Part 3: Oscillation method", the inhibition rates of Staphylococcus aureus, Escherichia coli, and Candida albicans of the antibacterial hemostatic gauzes prepared in Examples 1-3 and Comparative Examples 1-3 were sampled and tested, and the test results are as follows:

[0017] Performance test 2 The whole blood coagulation index (BCI) of the antibacterial hemostatic gauzes prepared in Examples 1-3 and Comparative Examples 1-3 was tested. The test method was as follows: Fresh blood of SD rats was drawn using a blood collection tube containing 3.8% sodium citrate anticoagulant. Seven 10 mL centrifuge tubes were taken, and 25 μL of anticoagulant-containing blood was added to each tube. Then, the antibacterial hemostatic gauzes prepared in Examples 1-3 and Comparative Examples 1-3 with an area of 2 cm × 2 cm were taken and added to 6 of the centrifuge tubes, which were used as test centrifuge tubes. The centrifuge tube without adding the gauze was used as a reference centrifuge tube. Each centrifuge tube was left standing at room temperature for 10 min, then 10 mL of purified water was added to each centrifuge tube, vortexed for 2 min, and then 200 μL of the sample was taken from each centrifuge tube and added to a 96-well plate. The absorbance value A was measured at 414 nm using an enzyme-linked immunosorbent assay (ELISA) reader. The BCI of the samples in each test centrifuge tube was calculated respectively. The calculation formula was as follows: BCI = absorbance value A of the sample in the test centrifuge tube / absorbance value A of the sample in the reference centrifuge tube × 100; The test was repeated 3 times, and the average value was taken. The calculation results are as follows:

[0018] Performance test 3 According to the national standard GB / T 20944.3-2008 "Evaluation of antibacterial properties of textiles - Part 3: Oscillation method", after sampling and testing the inhibition rates of Staphylococcus aureus, Escherichia coli, and Candida albicans of the antibacterial hemostatic gauzes prepared in Examples 1-3 and Comparative Examples 1-3, the remaining antibacterial hemostatic gauzes were stored in a light-proof environment at a temperature of 23 °C and a relative humidity of 60% for 30 d, and then the inhibition rates of Staphylococcus aureus, Escherichia coli, and Candida albicans of the antibacterial hemostatic gauzes were sampled and tested continuously. The decline rates of the inhibition rates of Staphylococcus aureus, Escherichia coli, and Candida albicans were calculated respectively. The calculation results are as follows:

[0019] Performance test 4 After the antibacterial hemostatic gauzes prepared in Examples 1-3 and Comparative Examples 1-3 were stacked 10 layers and fixed respectively, the water vapor transmission rates were tested respectively. The test results are as follows:

[0020] Performance test 5 According to the national standard GB / T 20944.3-2008 "Evaluation of antibacterial properties of textiles - Part 3: Oscillation method", the inhibition rates of Staphylococcus aureus, Escherichia coli, and Candida albicans of the antibacterial hemostatic gauzes prepared in Examples 1-3 and Comparative Examples 1-3 were sampled and tested. When sampling and testing, 10 samples were taken respectively, with an interval of 10 cm between each sample. Then, the differences between the maximum inhibition rate and the minimum inhibition rate of Staphylococcus aureus, the maximum inhibition rate and the minimum inhibition rate of Escherichia coli, and the maximum inhibition rate and the minimum inhibition rate of Candida albicans were calculated respectively. The calculation results are as follows:

[0021] Unless otherwise specified, the percentages used in the present invention are all mass percentages.

[0022] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or perform equivalent replacements for some of the technical features. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A method for preparing an antibacterial hemostatic gauze, characterized in that: include: Pretreatment, one-time immersion treatment, two-time immersion treatment, three-time immersion treatment, four-time immersion treatment, five-time immersion treatment, six-time immersion treatment, post-treatment; The one-time soaking treatment comprises completely soaking the pretreated gauze in 1,5-pentanediol, leaving it to stand at room temperature, taking out the gauze, and hanging it vertically to obtain the gauze after the one-time soaking treatment; The three-immersion treatment comprises completely immersing the gauze after the second immersion treatment in a tannic acid aqueous solution, leaving it to stand at room temperature, taking out the gauze, and hanging it vertically to obtain the gauze after the three-immersion treatment; The four-time soaking treatment comprises completely soaking the gauze after three-time soaking treatment in the zinc oxide dispersion, standing at room temperature, taking out the gauze, and hanging it vertically to obtain the gauze after four-time soaking treatment; The preparation method of the zinc oxide dispersion is as follows: adding zinc oxide to purified water, stirring at room temperature, adding α-lactalbumin, continuing stirring, adding 1,5-pentanediol, continuing stirring, and obtaining the zinc oxide dispersion.

2. The method for preparing the antibacterial hemostatic gauze according to claim 1, characterized in that: The pretreatment comprises washing the degreased pure cotton gauze with water and then drying it to obtain the pretreated gauze; In the pretreatment, the drying temperature is 115-125° C. and the drying time is 1.5-2 h.

3. The method for preparing the antibacterial hemostatic gauze according to claim 1, characterized in that: In the one immersion treatment, the mass volume ratio of the pretreated gauze to 1,5-pentanediol is 500g:3000-3200mL.

4. The method for preparing the antibacterial hemostatic gauze according to claim 1, characterized in that: The secondary soaking treatment comprises completely soaking the gauze after the primary soaking treatment in a zinc chloride aqueous solution, leaving it to stand at room temperature, taking out the gauze, and hanging it vertically to obtain the gauze after the secondary soaking treatment; In the secondary immersion treatment, the mass fraction of the zinc chloride aqueous solution is 10%; The mass volume ratio of the gauze pretreated in the first immersion treatment to the zinc chloride aqueous solution in the second immersion treatment is 500g:8500-9500mL.

5. The method for preparing the antibacterial hemostatic gauze according to claim 1, characterized in that: In the three immersion treatments, the mass fraction of the tannic acid aqueous solution is 5%; The mass volume ratio of the gauze pretreated in the one immersion treatment to the tannic acid aqueous solution in the three immersion treatments is 500g:8500-9500mL.

6. The method for preparing the antibacterial hemostatic gauze according to claim 1, characterized in that: In the preparation of the zinc oxide dispersion, the particle size of the zinc oxide is 50 nm; The dosage ratio of zinc oxide, purified water, α-lactalbumin and 1,5-pentanediol is 48-50g:8320-9300mL:3-3.2g:180-200mL.

7. The method for preparing the antibacterial hemostatic gauze according to claim 1, characterized in that: The mass volume ratio of the gauze pretreated in the first immersion treatment to the zinc oxide dispersion in the fourth immersion treatment is 500g:8500-9500mL.

8. The method for preparing the antibacterial hemostatic gauze according to claim 1, characterized in that: The five soaking treatments are as follows: the gauze after four soaking treatments is completely soaked in a carboxymethyl chitosan aqueous solution, left to stand at room temperature, the gauze is taken out, and is hung vertically to obtain the gauze after five soaking treatments; In the five immersion treatments, the mass fraction of the carboxymethyl chitosan aqueous solution is 2%; The molecular weight of carboxymethyl chitosan in the carboxymethyl chitosan aqueous solution is 30 kDa, the degree of deacetylation is 90%, and the degree of substitution is 0.75; The mass volume ratio of the gauze pretreated in the one immersion treatment to the carboxymethyl chitosan aqueous solution in the five immersion treatments is 500g:8500-9500mL.

9. The method for preparing the antibacterial hemostatic gauze according to claim 1, characterized in that: The six-time soaking treatment comprises completely soaking the gauze after five-time soaking treatment in a calcium chloride aqueous solution, leaving it to stand at room temperature, taking out the gauze, and hanging it vertically to obtain the gauze after six-time soaking treatment; In the six immersion treatments, the mass fraction of the calcium chloride aqueous solution is 5%; The mass volume ratio of the gauze pretreated in the one immersion treatment to the calcium chloride aqueous solution in the six immersion treatments is 500g:8500-9500mL.

10. The method for preparing the antibacterial hemostatic gauze according to claim 1, characterized in that: The post-treatment comprises irradiating the gauze after six times of immersion treatment under ultraviolet light, standing at room temperature, and drying to obtain antibacterial hemostatic gauze; In the post-treatment, the power of the ultraviolet lamp is 300W and the wavelength is 365nm; The drying temperature is 115-125°C and the drying time is 2.5-3h; The gauze after the six immersion treatments was placed at 80-90 cm under the ultraviolet lamp.

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