Solid tumor immune microenvironment detection and analysis device and method

Abstract

The invention relates to the technical field of tumor immune microenvironment detection and analysis, and discloses a solid tumor immune microenvironment detection and analysis device and method, and the solid tumor immune microenvironment detection and analysis device comprises a detection mechanism which is used for being in contact with a tumor sample or puncturing a part of the tumor sample and then uploading data for an operator to analyze; the transporting and cleaning mechanism integrates transporting, cleaning and adjusting functions; two sides of a sample are synchronously cleaned in the transporting process, and the transporting and cleaning mechanism adapts to different sample sizes; the clamping steering mechanism is used for clamping the sample and turning and positioning the sample to expose the cleaning surface; the collecting assembly can be detached to recover fallen impurities; and the wiping assembly is used for cleaning a detection area of the detection mechanism after detection, so as to prevent residual substances from influencing subsequent detection results. According to the transporting and cleaning mechanism, impurities on the outer sides of the samples can be automatically cleaned while the tumor samples are transported, meanwhile, adjustment can be conducted according to the samples of different sizes, and the accuracy of subsequent detection is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of tumor immune microenvironment detection and analysis, and particularly to an apparatus and method for detecting and analyzing the immune microenvironment of solid tumors. Background Art

[0002] The detection of the tumor immune microenvironment is of great significance in modern medical research and clinical diagnosis. With the progress of science and technology, in-depth analysis of the tumor immune microenvironment can help researchers better understand the occurrence mechanism of tumors and their interaction with the immune system, thus providing strong support for the early diagnosis and personalized treatment of tumors. To ensure the accuracy of detection results, a series of operations such as precise transportation, cleaning, clamping, and probe cleaning of tumor samples are required.

[0003] In the prior art, the transportation and cleaning of tumor samples are usually carried out separately. Most devices move samples from one area to another through a separate transportation system, and the cleaning work relies on another set of devices or manual operations. Although such a design can complete basic functions, it often has the problems of cumbersome operation procedures and low efficiency. Moreover, during the transportation of tumor samples, impurities are likely to adhere to the outside of the samples, thus affecting subsequent detection results. To solve this problem, some devices have added cleaning components to remove impurities and improve the cleanliness of samples. Nevertheless, the effects of these technologies are still limited to single operations and cannot achieve efficient integration.

[0004] However, there are still some deficiencies in the prior art; firstly, in the prior art, the transportation and cleaning operations are carried out separately, resulting in cumbersome equipment and complex operations. For the detection of the tumor immune microenvironment that requires high precision and efficiency, the separate operation procedures not only increase the manual burden but also easily cause sample contamination due to improper operation; secondly, the existing clamping systems generally can only provide simple clamping, lacking flipping and adjustment functions, and cannot accurately control the position and angle of tumor samples, easily causing incomplete exposure of the detection area; thirdly, most of the prior art ignores the cleaning of the residual substances on the periphery of the detection probe, which means that there may be a risk of cross-contamination after each use, resulting in inaccurate subsequent detections. Summary of the Invention

[0005] Aiming at the deficiencies of the prior art, the present invention provides an apparatus and method for detecting and analyzing the immune microenvironment of solid tumors, which solves the problems of cumbersome, low-efficiency, and easy sample contamination caused by the separate operations of tumor sample transportation, cleaning, clamping, and probe cleaning in the prior art.

[0006] To achieve the above object, the present invention is realized through the following technical solutions: An apparatus for detecting and analyzing the immune microenvironment of solid tumors, comprising: A detection mechanism, which is used to come into contact with a tumor sample or puncture a part of the tumor sample and then upload data for operators to analyze; A transportation and cleaning mechanism, which is arranged on the upper side of the detection mechanism. The transportation and cleaning mechanism includes a transportation component, a cleaning component and an adjustment component. The transportation component is used to provide support and storage for the tumor sample and transport the tumor sample to the detection area. The cleaning component is used to sweep both sides of the tumor sample during the transportation of the tumor sample to make the impurities adsorbed on the outside of the tumor sample fall off. The adjustment component is used to adjust the space for storing the tumor sample to accommodate tumor samples of different sizes; A clamping and turning mechanism, which is arranged on the upper side of the detection mechanism, used to clamp and move the tumor sample to the detection area and turn the tumor sample during the movement to expose the area of the tumor sample to be cleaned in the detection area; A collection component, which is arranged at the bottom of the detection mechanism, used to collect the impurities falling off the outside of the tumor sample and extract them later for unified cleaning; A wiping component, which is arranged inside the detection mechanism, used to clean the detection area of the detection mechanism after detection to prevent residual substances from affecting subsequent detection results.

[0007] Preferably, the detection mechanism includes a support frame. A frame is fixedly connected to the upper side of the support frame. A cylinder is fixedly connected to the top of the frame. The output end of the cylinder is fixedly connected to a detection probe.

[0008] Preferably, the transportation component includes a first hydraulic cylinder. The first hydraulic cylinder is fixedly connected to the upper side of the support frame. The output end of the first hydraulic cylinder is fixedly connected to a push plate. Two connecting plates are arranged on the other side of the push plate.

[0009] Preferably, the cleaning component includes two groups of brush rollers. The two groups of brush rollers are respectively rotatably connected to the middle parts of the two connecting plates. A connecting component is fixedly connected to the bottom of the brush roller. The connecting component is composed of a tensioning sprocket and a tensioning chain, used to rotate any group of brush rollers simultaneously. A gear is fixedly connected to the upper side of the brush roller. An L-shaped connecting block is fixedly connected to the outside of the connecting plate. Two limit blocks are slidably connected to the inner bottom of the frame. A rack is fixedly connected to the bottom of the limit block. The gear and the rack are meshed with each other. The side of the L-shaped connecting block away from the connecting plate is set as a T shape. The side of the L-shaped connecting block away from the connecting plate is slidably connected to the middle part of the rack.

[0010] Preferably, the adjustment component includes a bidirectional lead screw. The bidirectional lead screw is rotatably connected to the middle part of the push plate. Two nut seats are threadedly connected to the outer periphery of the bidirectional lead screw. The connecting plate is fixedly connected to the outside of the nut seat.

[0011] Preferably, the clamping and steering mechanism includes a second hydraulic cylinder which is fixedly connected to the upper side of the support frame. The output end of the second hydraulic cylinder is rotatably connected to a connecting rod. A convex block is fixedly connected to the outer side of the connecting rod. An arc-shaped groove is formed inside the frame. The convex block is slidably connected to the middle of the arc-shaped groove. One end of the connecting rod away from the second hydraulic cylinder is fixedly connected to a casing. A connecting disk is fixedly connected to the other side of the casing. A motor is fixedly connected to the inside of the casing. The output end of the motor is fixedly connected to a turntable which is rotatably connected to the middle of the connecting disk. An arc-shaped plate is rotatably connected to the outer side of the turntable. A slider is rotatably connected to the other side of the arc-shaped plate. A clamping plate is fixedly connected to the other side of the slider.

[0012] Preferably, the collection assembly includes two L-shaped support blocks which are fixedly connected to the bottom of the support frame. A collection box is slidably connected between the two L-shaped support blocks.

[0013] Preferably, the wiping assembly includes a sleeve which is fixedly connected to the inner bottom of the frame. A plurality of groups of bristles are fixedly connected to the inside of the sleeve. The bristles are in contact with the detection probes.

[0014] Preferably, a chute is formed in the middle of the connecting disk. The slider is slidably connected to the middle of the chute.

[0015] The present invention also provides a method for using the detection and analysis device for the immune microenvironment of solid tumors, including the following steps: Step 1: Rotate the bidirectional lead screw forward or backward to make the nut seat drive the two connecting plates to approach or move away from each other. After adjusting to the size where the current tumor sample can be placed, place the tumor sample between the two connecting plates. At this time, drive the first hydraulic cylinder to push the push plate and the connecting plate to move. When the push plate moves, through the limitation of the limiting block and within the sliding range provided by the L-shaped connecting block, the engagement between the gear and the rack can be maintained when the connecting plate moves, and the gear can drive the brush roller to rotate. When one of the brush rollers rotates, through the design of the tensioning sprocket and the tensioning chain, multiple brush rollers can rotate simultaneously, so as to clean the impurities attached to both sides of the tumor sample. Step 2: After the transportation and cleaning mechanism cleans and transports the tumor sample into the device, drive the second hydraulic cylinder to make the connecting rod drive the clamping part to move. While moving, through the limitation of the arc-shaped groove and the cooperation of the convex block, the connecting rod can be flipped, so that the clamping part is in a vertical state, and after flipping, it moves to the tumor sample area. At this time, drive the driving motor to rotate the turntable. During the rotation process, drive the arc-shaped plate to displace. At the same time, due to the limitation of the slider, the arc-shaped plate can drive the two clamping plates to move in parallel and clamp the tumor sample. After clamping, retract the connecting rod through the second hydraulic cylinder, and during the retraction process, make the clamping part flip and be in a parallel state. At this time, it is convenient for the detection probe to detect the tumor sample; Step 3: During detection, drive the detection probe to descend through the bristles and contact or puncture into the tumor sample for collection and detection through the driving cylinder. After the detection is completed, drive the driving cylinder to make the detection probe rise into the sleeve and be blocked by the bristles, so that the substances remaining on the outer periphery of the detection probe can be removed; Step 4: When the collected impurities need to be cleaned, pull the collection box, and under the limitation of the L-shaped support block, the collection box can be drawn out in parallel and the impurities collected in it can be cleaned. Under the positioning of the L-shaped support block, the collection box can be quickly put into use.

[0016] The present invention provides a device and method for detecting and analyzing the immune microenvironment of solid tumors. It has the following beneficial effects: 1. The transportation and cleaning mechanism of the present invention combines the functions of transportation, cleaning and adjustment. It can automatically clean the impurities outside the sample while transporting the tumor sample, avoiding the influence of impurities on the tumor detection effect. At the same time, compared with the prior art that can only transport and clean separately, this integrated design saves time and is efficient, directly improving the accuracy of subsequent detection.

[0017] 2. The design of the clamping and turning mechanism can clamp and flip the tumor sample, and flip the area after cleaning the tumor sample to the bottom of the detection probe, making the sample more accurate during detection.

[0018] 3. Through the combination of the bristles and the sleeve, after each detection, the bristles will clean the substances on the outer periphery of the detection probe, ensuring no cross-contamination and preventing impurities from remaining on the outer periphery of the detection probe, further improving the accuracy of the detection result. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a three-dimensional view of the present invention; Figure 2 is a schematic cross-sectional view of the framework of the present invention; Figure 3 is a schematic structural view of the wiping assembly of the present invention; Figure 4 is a schematic cross-sectional view of the connecting plate of the present invention; Figure 5 Schematic diagram of the arc-shaped groove of the present invention; Figure 6 Schematic diagram of the bump of the present invention; Figure 7 Schematic diagram of the arc-shaped plate of the present invention; Figure 8 Schematic diagram of the collection assembly of the present invention.

[0020] Among them, 10, detection mechanism; 101, support frame; 102, frame; 103, cylinder; 104, detection probe; 20, transportation and cleaning mechanism; 201, first hydraulic cylinder; 202, push plate; 203, connecting plate; 204, brush roller; 205, tensioning sprocket; 206, tensioning chain; 207, gear; 208, L-shaped connecting block; 209, rack; 210, limit block; 211, bidirectional lead screw; 212, nut seat; 30, clamping and steering mechanism; 301, second hydraulic cylinder; 302, connecting rod; 303, bump; 304, arc-shaped groove; 305, housing; 306, connecting disk; 307, motor; 308, turntable; 309, arc-shaped plate; 310, slider; 311, clamping plate; 40, collection assembly; 401, L-shaped support block; 402, collection box; 50, wiping assembly; 501, sleeve; 502, bristles. Detailed implementation manners

[0021] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the specification of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0022] Please refer to Figures 1-8 , an apparatus for detecting and analyzing the immune microenvironment of solid tumors provided by an embodiment of the present invention includes: A detection mechanism 10 for contacting with a tumor sample or puncturing a part of the tumor sample and then uploading data for an operator to analyze; A transportation and cleaning mechanism 20, which is arranged on the upper side of the detection mechanism 10. The transportation and cleaning mechanism 20 includes a transportation component, a cleaning component, and an adjustment component. The transportation component is used to provide support and storage for the tumor sample and transport the tumor sample to the detection area. The cleaning component is used to sweep both sides of the tumor sample during the transportation of the tumor sample, so that the impurities adsorbed on the outside of the tumor sample fall off. The adjustment component is used to adjust the space for storing the tumor sample to accommodate tumor samples of different sizes; The clamping steering mechanism 30 is arranged on the upper side of the detection mechanism 10 and is used to clamp and move the tumor sample to the detection area, and flip the tumor sample during the movement so that the area to be cleaned of the tumor sample is exposed in the detection area; The collection component 40 is arranged at the bottom of the detection mechanism 10 and is used to collect the impurities shed from the outside of the tumor sample and extract them for unified cleaning later; The wiping component 50 is arranged inside the detection mechanism 10 and is used to clean the detection area of the detection mechanism 10 after detection to prevent residual substances from affecting subsequent detection results.

[0023] Please refer to Figure 1 、 Figure 2 and Figure 5 In a preferred embodiment of the present invention, the detection mechanism 10 includes a support frame 101. A frame 102 is fixedly connected to the upper side of the support frame 101. A cylinder 103 is fixedly connected to the top of the frame 102. The output end of the cylinder 103 is fixedly connected to a detection probe 104. The support frame 101 is used to support the device for the convenience of operation by the operator. The cylinder 103 is used to adjust the height of the detection probe 104. When the cylinder 103 is driven, the detection probe 104 moves downward and contacts the tumor sample to complete the detection operation. The detection operation of the detection probe 104 is prior art and will not be elaborated in detail in this text.

[0024] Please refer to Figure 1 、 Figure 2 and Figure 4 In a preferred embodiment of the present invention, the transportation component includes a first hydraulic cylinder 201. The first hydraulic cylinder 201 is fixedly connected to the upper side of the support frame 101. The output end of the first hydraulic cylinder 201 is fixedly connected to a push plate 202. Two connecting plates 203 are arranged on the other side of the push plate 202. The connecting plates 203 are used to store and support the tumor sample. At the same time, the first hydraulic cylinder 201 is used to drive the movement of the push plate 202 and the connecting plates 203, so as to transport the tumor into the device and wait for the detection operation to be carried out.

[0025] Please refer to Figure 4 and Figure 5, in a preferred embodiment of the present invention, the cleaning component includes two groups of brush rollers 204. The two groups of brush rollers 204 are respectively rotatably connected to the middle parts of two connecting plates 203. A connecting component is fixedly connected to the bottom of the brush roller 204. The connecting component is composed of a tensioning sprocket 205 and a tensioning chain 206, and is used to rotate any group of brush rollers 204 simultaneously. Through the setting of the connecting component, when one of the brush rollers 204 rotates, it can drive all the brush rollers 204 in this group to rotate, so as to complete the cleaning of the tumor sample. At the same time, the connecting component is a prior art and will not be elaborated in this text. A gear 207 is fixedly connected to the upper side of the brush roller 204. An L-shaped connecting block 208 is fixedly connected to the outside of the connecting plate 203. Two limiting blocks 210 are slidably connected to the inner bottom of the frame 102. A rack 209 is fixedly connected to the bottom of the limiting block 210. The gear 207 and the rack 209 are meshed with each other. Through the setting of the limiting block 210, the position of the rack 209 can be adjusted, and the connection with the gear 207 can be maintained, so as to ensure that while transporting the tumor, the gear 207 drives the brush roller 204 to rotate, thereby completing the cleaning operation on the outside of the tumor sample. The side of the L-shaped connecting block 208 away from the connecting plate 203 is set to be T-shaped. The side of the L-shaped connecting block 208 away from the connecting plate 203 is slidably connected to the middle of the rack 209. Through the setting of the L-shaped connecting block 208 and the T-shaped setting, when the connecting plate 203 is adjusted, the rack 209 can be driven to be adjusted simultaneously.

[0026] Please refer to Figure 4 , in a preferred embodiment of the present invention, the adjusting component includes a bidirectional lead screw 211. The bidirectional lead screw 211 is rotatably connected to the middle of the push plate 202. Two nut seats 212 are threadedly connected to the outer periphery of the bidirectional lead screw 211. The connecting plate 203 is fixedly connected to the outside of the nut seat 212. By rotating the bidirectional lead screw 211, the nut seat 212 can drive the connecting plate 203 to move closer to or away from each other, so as to adjust the distance between the two connecting plates 203 according to the size of the tumor sample, and enable the cleaning component to clean the outside of the tumor sample of any size.

[0027] Please refer to Figures 5-7, in a preferred embodiment of the present invention, the clamping and steering mechanism 30 includes a second hydraulic cylinder 301. The second hydraulic cylinder 301 is fixedly connected to the upper side of the support frame 101. The output end of the second hydraulic cylinder 301 is rotatably connected to a connecting rod 302. A convex block 303 is fixedly connected to the outside of the connecting rod 302. An arc-shaped groove 304 is formed inside the frame 102. The convex block 303 is slidably connected to the middle of the arc-shaped groove 304. One end of the connecting rod 302 away from the second hydraulic cylinder 301 is fixedly connected to a housing 305. A connecting disc 306 is fixedly connected to the other side of the housing 305. A motor 307 is fixedly connected to the inside of the housing 305. The output end of the motor 307 is fixedly connected to a turntable 308. The turntable 308 is rotatably connected to the middle of the connecting disc 306. An arc-shaped plate 309 is rotatably connected to the outside of the turntable 308. A slider 310 is rotatably connected to the other side of the arc-shaped plate 309. A clamping plate 311 is fixedly connected to the other side of the slider 310. Through the rotational design of the connecting rod 302, it is allowed to rotate the connecting rod 302 when driven by the second hydraulic cylinder 301. Under the limitation of the arc-shaped groove 304, when the second hydraulic cylinder 301 drives the connecting rod 302 to move forward, the connecting rod 302 is flipped by extrusion, so that the two clamping plates 311 can be changed from the left and right sides to the up and down sides for clamping. At this time, the driving motor 307 rotates the turntable 308. While it rotates, the arc-shaped plate 309 can only drive the clamping plate 311 to move parallelly through the limitation of the slider 310, so as to clamp the tumor sample. When the second hydraulic cylinder 301 drives the connecting rod 302 to reset after clamping, the connecting rod 302 can be reversely flipped and reset, so that the side for cleaning the tumor sample is perpendicular to the bottom of the detection probe 104, thereby improving the detection accuracy.

[0028] Please refer to Figure 1 and Figure 8 , in a preferred embodiment of the present invention, the collection assembly 40 includes two L-shaped support blocks 401. The L-shaped support blocks 401 are fixedly connected to the bottom of the support frame 101. A collection box 402 is slidably connected between the two L-shaped support blocks 401. The provided L-shaped support blocks 401 can limit the collection box 402 and at the same time provide support for the collection box 402, prevent the collection box 402 from falling and provide a slidable space for the collection box 402. By sliding the collection box 402, the collection box 402 can be quickly assembled or disassembled and cleaned.

[0029] Please refer to Figure 2 and Figure 3 , in a preferred embodiment of the present invention, the wiping assembly 50 includes a sleeve 501. The sleeve 501 is fixedly connected to the inner bottom of the frame 102. A plurality of groups of bristles 502 are fixedly connected to the inside of the sleeve 501. The bristles 502 are in contact with the detection probe 104. The provided bristles 502 can remove the substances remaining on the outer periphery of the detection probe 104 when the detection probe 104 is retracted, thereby improving the accuracy of the next detection.

[0030] Please refer to Figure 6 Figure 6 In a preferred embodiment of the present invention, a chute is provided in the middle of the connecting plate 306, and the slider 310 is slidably connected to the middle of the chute. The chute can limit the slider 310, so that the slider 310 only drives the clamping plate 311 to move parallelly, avoiding the inclination or deviation of the clamping plate 311 and improving the clamping accuracy and effect.

[0031] The present invention also provides a method for using an apparatus for detecting and analyzing the immune microenvironment of solid tumors, including the following steps: Step 1: Rotate the bidirectional lead screw 211 forward or backward to make the nut seat 212 drive the two connecting plates 203 to approach or move away from each other. After adjusting to the size where the current tumor sample can be placed, place the tumor sample between the two connecting plates 203. At this time, drive the hydraulic cylinder 201 to move the push plate 202 and the connecting plate 203. When the push plate 202 moves, through the limitation of the limiting block 210 and within the sliding range provided by the L-shaped connecting block 208, the engagement between the gear 207 and the rack 209 can be maintained when the connecting plate 203 moves, and the gear 207 drives the brush roller 204 to rotate. When one of the brush rollers 204 rotates, through the design of the tensioning sprocket 205 and the tensioning chain 206, multiple brush rollers 204 can rotate simultaneously, so as to clean the impurities attached to both sides of the tumor sample; Step 2: When the transportation and cleaning mechanism 20 cleans and transports the tumor sample to the inside of the apparatus, drive the hydraulic cylinder 301 to make the connecting rod 302 drive the clamping part to move. During the movement, through the limitation of the arc-shaped groove 304 and the cooperation of the convex block 303, the connecting rod 302 can be turned over, so that the clamping part is in a vertical state, and after turning over, it moves to the tumor sample area. At this time, drive the driving motor 307 to rotate the turntable 308. During the rotation process, drive the arc-shaped plate 309 to displace. At the same time, due to the limitation of the slider 310, the arc-shaped plate 309 can drive the two clamping plates 311 to move parallelly and clamp the tumor sample. After clamping, drive the hydraulic cylinder 301 to retract the connecting rod 302, and during the retraction process, the clamping part is turned over and in a parallel state. At this time, it is convenient for the detection probe 104 to detect the tumor sample; Step 3: During detection, drive the detection probe 104 to descend through the bristles 502 by the driving cylinder 103 and contact or puncture into the tumor sample for collection and detection. After the detection is completed, drive the driving cylinder 103 to make the detection probe 104 rise into the sleeve 501 and be blocked by the bristles 502, so as to remove the substances remaining on the outer periphery of the detection probe 104; Step 4: When the collected impurities need to be cleaned, the collection box 402 can be pulled out in parallel under the limit of the L-shaped support block 401 to clean the impurities collected therein, and the collection box 402 can be quickly put into use under the positioning of the L-shaped support block 401.

[0032] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A solid tumor immune microenvironment detection and analysis device, characterized in that: include: A detection mechanism (10), used to contact with the tumor sample or puncture part of the tumor sample and upload data for analysis by an operator; A transport and cleaning mechanism (20) is arranged on the upper side of the detection mechanism (10), the transport and cleaning mechanism (20) comprising a transport component, a cleaning component and an adjustment component, the transport component being used to provide support and storage for tumor samples and to transport the tumor samples to the detection area, the cleaning component being used to clean both sides of the tumor samples during the process of transporting the tumor samples so as to remove impurities adsorbed on the outer side of the tumor samples, and the adjustment component being used to adjust the space for storing the tumor samples, so as to be suitable for tumor samples of different sizes; a clamping and steering mechanism (30), which is arranged on the upper side of the detection mechanism (10) and is used to clamp the tumor sample and move it to the detection area, and to flip the tumor sample during the movement so that the cleaned area of ​​the tumor sample is exposed to the detection area; A collection component (40), which is arranged at the bottom of the detection mechanism (10) and is used to collect impurities that fall off the outside of the tumor sample and subsequently extract them for unified cleaning; A wiping component (50) is arranged inside the detection mechanism (10) and is used to clean the detection area of ​​the detection mechanism (10) after detection to prevent residual substances from affecting subsequent detection results.

2. A solid tumor immune microenvironment detection and analysis device according to claim 1, characterized in that: The detection mechanism (10) comprises a support frame (101), the upper side of the support frame (101) is fixedly connected to a frame (102), the top of the frame (102) is fixedly connected to a cylinder (103), and the output end of the cylinder (103) is fixedly connected to a detection probe (104).

3. A solid tumor immune microenvironment detection and analysis device according to claim 2, characterized in that: The transport component comprises a hydraulic cylinder 1 (201), wherein the hydraulic cylinder 1 (201) is fixedly connected to the upper side of the support frame (101), an output end of the hydraulic cylinder 1 (201) is fixedly connected to a push plate (202), and two connecting plates (203) are arranged on the other side of the push plate (202).

4. A solid tumor immune microenvironment detection and analysis device according to claim 3, characterized in that: The cleaning assembly comprises two groups of brush sticks (204), the two groups of brush sticks (204) are rotatably connected to the middle parts of the two connecting plates (203), the bottom of the brush sticks (204) is fixedly connected to a connecting assembly, the connecting assembly consists of a tensioning sprocket (205) and a tensioning chain (206), and is used to make any group of brush sticks (204) rotate simultaneously, the upper side of the brush stick (204) is fixedly connected to a gear (207), and the outer side of the connecting plate (203) is fixedly connected to the gear (207). An L-shaped connection block (208) is provided, the inner bottom of the frame (102) is slidably connected to two limit blocks (210), the bottom of the limit block (210) is fixedly connected to a rack (209), the gear (207) and the rack (209) are meshed with each other, the side of the L-shaped connection block (208) away from the connection plate (203) is set to be T-shaped, and the side of the L-shaped connection block (208) away from the connection plate (203) is slidably connected to the middle part of the rack (209).

5. A solid tumor immune microenvironment detection and analysis device according to claim 4, characterized in that: The adjustment assembly comprises a bidirectional screw rod (211), the bidirectional screw rod (211) being rotatably connected to the middle part of the push plate (202), the outer periphery of the bidirectional screw rod (211) being threadedly connected to two nut seats (212), and the connecting plate (203) being fixedly connected to the outer sides of the nut seats (212).

6. A solid tumor immune microenvironment detection and analysis device according to claim 5, characterized in that: The clamping and steering mechanism (30) comprises a second hydraulic cylinder (301), wherein the second hydraulic cylinder (301) is fixedly connected to the upper side of the support frame (101), the output end of the second hydraulic cylinder (301) is rotatably connected to a connecting rod (302), the outer side of the connecting rod (302) is fixedly connected to a protrusion (303), an arc-shaped groove (304) is provided inside the frame (102), the protrusion (303) is slidably connected to the middle part of the arc-shaped groove (304), and the end of the connecting rod (302) away from the second hydraulic cylinder (301) is fixedly connected to A housing (305) is provided, the other side of the housing (305) being fixedly connected to a connection disk (306), the interior of the housing (305) being fixedly connected to a motor (307), the output end of the motor (307) being fixedly connected to a rotating disk (308), the rotating disk (308) being rotatably connected to the middle of the connection disk (306), the outer side of the rotating disk (308) being rotatably connected to an arc-shaped plate (309), the other side of the arc-shaped plate (309) being rotatably connected to a slider (310), and the other side of the slider (310) being fixedly connected to a clamping plate (311).

7. A solid tumor immune microenvironment detection and analysis device according to claim 6, characterized in that: The collecting assembly (40) comprises two L-shaped supporting blocks (401), wherein the L-shaped supporting blocks (401) are fixedly connected to the bottom of the supporting frame (101), and a collecting box (402) is slidably connected between the two L-shaped supporting blocks (401).

8. A solid tumor immune microenvironment detection and analysis device according to claim 7, characterized in that: The wiping assembly (50) comprises a sleeve (501), the sleeve (501) being fixedly connected to the inner bottom of the frame (102), a plurality of groups of bristles (502) being fixedly connected inside the sleeve (501), the bristles (502) being in contact with the detection probe (104).

9. A solid tumor immune microenvironment detection and analysis device according to claim 8, characterized in that: A sliding groove is provided in the middle of the connection plate (306), and the sliding block (310) is slidably connected to the middle of the sliding groove.

10. A method for using a solid tumor immune microenvironment detection and analysis device, characterized in that: Using the solid tumor immune microenvironment detection and analysis device according to claim 9 comprises the following steps: Step 1: by rotating the bidirectional screw rod (211) in the forward or reverse direction, the nut seat (212) and the two connecting plates (203) are moved closer to or farther from each other. After the size of the tumor sample is adjusted to be able to be placed, the tumor sample is placed between the two connecting plates (203). At this time, the push plate (202) and the connecting plate (203) are moved by driving the hydraulic cylinder 1 (201). When the push plate (202) moves, the limit of the limit block (210) and the sliding range provided by the L-shaped connecting block (208) can maintain the meshing between the gear (207) and the rack (209) when the connecting plate (203) moves, and the gear (207) drives the brush rod (204) to rotate. When one of the brush rods (204) rotates, the design of the tensioning sprocket (205) and the tensioning chain (206) can cause multiple brush rods (204) to rotate simultaneously, so that impurities attached to both sides of the tumor sample can be cleaned; Step 2: After the transport and cleaning mechanism (20) cleans and transports the tumor sample to the inside of the device, the second hydraulic cylinder (301) is driven to make the connecting rod (302) drive the clamping part to move. During the movement, the connecting rod (302) can be flipped through the limit of the arc groove (304) and the cooperation of the protrusion (303), so that the clamping part is in a vertical state, and moves to the tumor sample area after flipping. At this time, the turntable (308) is rotated by the driving motor (307), and the arc plate (309) is driven to move during the rotation. At the same time, due to the limit of the slider (310), the arc plate (309) can drive the two clamping plates (311) to move in parallel and clamp the tumor sample. After clamping, the connecting rod (302) is retracted by driving the second hydraulic cylinder (301), and the clamping part is flipped and in a parallel state during the retraction process, which facilitates the detection probe (104) to detect the tumor sample; Step 3: During detection, the cylinder (103) is driven to make the detection probe (104) descend through the bristles (502) and contact the tumor sample or puncture the tumor sample for collection and detection. After the detection is completed, the cylinder (103) is driven to make the detection probe (104) ascend into the sleeve (501) and be blocked by the bristles (502), so that the residual substances on the periphery of the detection probe (104) can be removed; Step 4: When the collected impurities need to be cleaned, the collection box (402) can be pulled out in parallel and the collected impurities can be cleaned under the limit of the L-shaped support block (401). The collection box (402) can be quickly put into use under the positioning of the L-shaped support block (401).

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