Automated endotoxin detection device and detection method thereof

Abstract

The present invention relates to the technical field of detection devices and detection methods thereof, and proposes an automated endotoxin detection device and a detection method thereof, which can form multiple relatively independent reaction spaces, and can successively form endotoxin detection operations for multiple samples while ensuring basic endotoxin detection. It has a high degree of automation, is more convenient to use, has higher efficiency, and consumes less energy. It includes a base, a constant temperature box and multiple carrier boxes. The base is provided with an installation strip, and the constant temperature box is provided with two covering pipes, which are respectively connected to a water inlet pipe and a return pipe. A circulation pump and a heater are installed in the base, and the water inlet pipe and the return pipe are respectively connected to the pump inlet and the pump outlet of the circulation pump. The heater is equipped with a water inlet pipe to form a covering heating, and a partition isolation structure is installed in the constant temperature box. The partition isolation structure divides the constant temperature box into multiple inlets, and the multiple carrier boxes all include a bottom shell and a top cover, and the multiple bottom shells are all provided with an insertion fixing frame.

Description

Technical Field

[0001] The present invention relates to the technical field of detection devices and detection methods thereof, and in particular to an automated endotoxin detection device and a detection method thereof. Background Art

[0002] As we all know, the gel-based horseshoe crab reagent is an important biological reagent, mainly used to detect endotoxins. Its working principle is based on the phenomenon that the coagulation protein in the blood of the marine organism "horseshoe crab" reacts specifically with bacterial endotoxins to form a gel. The speed of this reaction and the firmness of the gel are related to the concentration of endotoxins. By observing the formation of the gel, the content of endotoxins in the bacterial sample can be detected. In order to facilitate the automated detection of endotoxins, we propose an automated endotoxin detection device and its detection method.

[0003] After searching, the Chinese patent publication number CN218646986U and the Chinese patent publication number CN210442378U respectively disclose a serum endotoxin detection device and an endotoxin detection device for the Limulus amebocyte lysate reagent method, wherein the former is roughly described as including a base, the base is connected to a detection box, the detection box is connected to a cover body, the cover body is provided with a snap and an LED light, the detection box is provided with a detection plate, a recovery tank, a water bath and a heating device, the detection plate is provided with a handle and has a test tube hole, the test tube hole is equipped with an arc clip, the arc clip is connected to a connecting rod, the detection plate has a spring hole, the spring hole is connected to a spring, the detection plate is connected to a lifting plate, the bottom of the lifting plate is connected to a hydraulic rod, the base is provided with a control panel, the control panel is provided with a digital thermometer, a temperature adjustment knob, a power switch, a light switch, a pause switch, an up switch and a down switch, and when in use, the temperature adjustment knob is adjusted to control the temperature of the water in the water bath within an appropriate range. , add the prepared horseshoe crab reagent, the sample to be tested and the comparison sample into the test tube in a certain proportion according to the experimental requirements, and then put the test tube into the test tube hole respectively. The curvature of the arc clamp fits the size of the test tube, and the test tube is clamped under the elastic force of the spring to prevent the test tube from falling off from the test tube hole. Cover the lid and heat it in a water bath for the time required by the experiment. Adjust the hydraulic rod control switch. After the detection plate rises to a certain height, hold the handle to rotate the detection plate to a certain angle, turn on the light switch, and observe the gel shedding with the assistance of the LED light. The latter can be roughly described as including an ampoule rack, a constant temperature water bath device, a camera device and a display device electrically connected to the camera device. The ampoule rack includes a rotating rod for placing the ampoule, and a clamping mechanism for clamping the ampoule is provided on the rotating rod. The rotating rod is connected to a driving device for driving the rotating rod to rotate. When in use, after the ampoule has been in the water bath for one hour, the ampoule is inverted by the detection device, and images are taken before and after the inversion.

[0004] Although both of the above-mentioned two sets of existing technical solutions can realize the detection of endotoxins, the actual detection process usually involves the detection of multiple different samples, and in order to ensure the reliability of the detection data, the same sample is sometimes tested multiple times. The detection space in the above-mentioned two sets of technical solutions is relatively fixed and single, that is, once loaded, it needs to wait for the complete water bath reaction before it can be taken out. Since the endotoxin detection process requires a long time of water bath insulation, the convenience of use is poor. Summary of the Invention

[0005] In response to the shortcomings of the existing technology, the present invention provides an automated endotoxin detection device and a detection method thereof, which can form multiple relatively independent reaction spaces, and can successively form endotoxin detection operations for multiple samples while ensuring basic endotoxin detection. It has a high degree of automation, is more convenient to use, has higher detection efficiency, and consumes less energy.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an automated endotoxin detection device, comprising a base, a thermostat and a plurality of carrier boxes, the base being provided with a mounting strip, two covering pipes being provided in the thermostat, the two covering pipes being connected to a water inlet pipe and a water return pipe respectively, a circulating pump and a heater being installed in the base, the water inlet pipe and the water return pipe being connected to a pump inlet and a pump outlet of the circulating pump respectively, the heater being equipped with a matching water inlet pipe to form a covering heating, a partition isolation structure being installed in the thermostat, the partition isolation structure dividing the thermostat into a plurality of inlets, and the plurality of carrier boxes each comprising Including a bottom shell and a top cover, multiple top covers are respectively provided with insertion contact seals between the multiple bottom shells, multiple bottom shells are respectively provided with insertion fixing frames, multiple bottom shells are respectively matched with the multiple insertion ports, a take-and-place port is opened on the constant temperature box, a curved plate door is rotatably connected in the take-and-place port, a cutting-in follow-up structure is installed on the curved plate door, multiple top covers are respectively provided with insertion ports matching the cutting-in follow-up structure, an automatic lifting structure is provided in the constant temperature box, the automatic lifting structure is used for the movement and adjustment of multiple bottom shells, two side brackets are fixedly connected to the base, and the two side brackets are used for the bracket installation of the automatic lifting structure.

[0007] Preferably, the constant temperature box includes a water tank and an insulation cover, the water tank is fixedly connected to the mounting strip, the insulation cover is fixedly connected to the base, a contact seal is provided between the insulation cover and the water tank, the two covering tubes are both provided in the water tank, and the access port is opened on the insulation cover.

[0008] Preferably, the partition isolation structure includes a fixing bar, which is fixedly connected in the water tank. The fixing bar is fixedly connected to a plurality of partition plates, and the plurality of partition plates are fixedly connected in the water tank. The heights of the plurality of partition plates are less than the depth of the water tank, and the tops of the plurality of partition plates are flush with the top of the water tank. The inlet is the area between two adjacent partition plates, and a rotating isolation plate is rotatably connected between each two adjacent partition plates. The bottom ends of the plurality of rotating isolation plates are fixedly connected to return springs, and the plurality of return springs are fixedly connected in the water tank.

[0009] Preferably, the cutting-in follow-up structure includes an external hand ring, an internal slide and a rotating hook rod, the external hand ring is fixedly connected to the bent plate door, an installation cavity is provided on the bent plate door, a track groove is provided in the installation cavity, the internal slide is slidably connected in the track groove, and an elastic spring is fixedly connected in the track groove, the elastic spring is fixedly connected to the internal slide, the rotating hook rod is fixedly connected with a rotating cylinder, the rotating cylinder is rotatably connected to the internal slide, the rotating cylinder is located in the installation cavity, the internal slide is slidably connected with a screw-mouth drive rod, a through plate is provided in the screw-mouth drive rod, the through plate is fixedly connected in the rotating cylinder, and a progressive drive mechanism is installed in the bent plate door, and the progressive drive mechanism is used to drive the screw-mouth drive rod.

[0010] Preferably, the progressive drive mechanism includes a swing sleeve and a track ring sleeve, the swing sleeve is rotatably connected to the curved plate door, the swing sleeve is slidably connected with a telescopic bar, the telescopic bar is fixedly connected with a connecting spring, the connecting spring is fixedly connected to the swing sleeve, the telescopic bar is fixedly connected with a transmission rod, the transmission rod is inserted into the track ring sleeve, the track ring sleeve is fixedly connected to the internal slide, the swing sleeve is fixedly connected to an outward operating rod, the outward operating rod is fixedly connected with a first rotating link, the first rotating link is fixedly connected to a limiting spring, the limiting spring is fixedly connected to a second rotating link, and the second rotating link is rotatably connected to the external hand ring.

[0011] Preferably, the automatic lifting structure includes a servo motor, a lead screw and a plurality of optical bars, the lead screw is rotatably connected between the two side brackets, the plurality of optical bars are fixedly connected between the two side brackets, the servo motor is installed on one of the two side brackets, and the output shaft of the servo motor is fixedly connected to the lead screw, a wire sleeve is threadedly connected to the lead screw, and a movable frame is fixedly connected to the outside of the wire sleeve, a plurality of optical holes are opened on the movable frame, and the plurality of optical holes are respectively matched with a plurality of optical bars, the movable frame is slidably connected to a lifting frame, the movable frame is equipped with an external pressure sensor, and a plane matching the external pressure sensor is provided at the top of the lifting frame, a magnetic drive assembly is installed between the lifting frame and the movable frame, the lifting frame is fixedly connected with a bar hook cylinder, the bar hook cylinder is equipped with a plurality of track parts, and the plurality of track parts are respectively fixedly connected to a plurality of bottom shells, and two pressure alignment assemblies are installed on the lifting frame.

[0012] Preferably, the magnetic drive assembly includes a permanent magnet and multiple electromagnets. A mounting slot is provided on the lifting frame, the permanent magnet is fixedly connected in the mounting slot, the multiple electromagnets are fixedly connected to the moving frame, and the multiple electromagnets are matched with the permanent magnet.

[0013] Preferably, the pressure alignment component includes two lifting hooks and two built-in pressure sensors, the two built-in pressure sensors are installed on the lifting frame, the two lifting hooks are slidably connected to the lifting frame, the two lifting hooks are fixedly connected with auxiliary springs, the two auxiliary springs are fixedly connected with auxiliary push bars, the two auxiliary push bars are matched with the two built-in pressure sensors respectively, and the two auxiliary push bars are slidably matched with the lifting frame.

[0014] Preferably, the top of the track member is provided with two upper gradual slopes, which respectively match the two lifting hooks, and the bottom end of the track member is provided with two lower gradual slopes, which respectively match the two ends of the bar hook tube, and the fixed bar is provided with a limiting surface that matches the rotating isolation plate, and the front end of the extended operating rod is rotatably connected to a holding ball.

[0015] A detection method of an automated endotoxin detection device:

[0016] S1. Before use, the control circuit is installed in the automated endotoxin detection device, and warm water is pre-installed in the water tank. When the circulating pump is powered on, the warm water in the water tank is sucked out through the return pipe and re-pumped into the water tank through the inlet pipe. As the warm water passes through the inlet pipe, it is heated by the heater to increase its temperature, thereby ensuring that the temperature of the warm water in the water tank is maintained.

[0017] S2. During use, the automatic lifting structure can be used to lift and move a single bottom shell among the multiple bottom shells, so that the bottom shell can be adjusted in position between the insertion port and the access port. When the carrier box needs to be loaded with a sample, the automatic lifting structure is used to transfer the carrier box to the access port. The top cover at the access port is connected to the curved door by adjusting the cut-in follower structure. Then, when the curved door is opened, the top cover can be opened synchronously with the opening of the curved door. After opening, the test tube pre-loaded with the sample and the horseshoe crab reagent is inserted into the fixed rack.

[0018] S3. After the test tube is loaded, the curved plate door is closed relative to the access port, so that the bottom shell corresponding to the top cover is synchronously closed, and the top cover and the curved plate door are separated by adjusting the cut-in follower structure to reset. Thereafter, the automatic lifting mechanism continues to work to control the bottom shell and the top cover to reset the carrier box as a whole from the access port to the corresponding insertion port. When the bottom shell is placed in the insertion port, the bottom of the bottom shell contacts the warm water in the water storage tank, and the sample and the limulus amebocyte lysate in the bottom shell are heated by heat conduction;

[0019] S4. Complete the loading of multiple batches of samples into multiple carrier boxes in sequence according to the above steps. When the sample insulation reaction in the carrier box is completed, the corresponding carrier box is transferred from the placement entrance to the placement entrance again through the automatic lifting structure, and finally the samples and horseshoe crab reagents after the reaction are completed are taken out, and the samples and horseshoe crab reagents after the reaction in the test tube are compared and judged to form an endotoxin detection.

[0020] Compared with the prior art, the present invention provides an automated endotoxin detection device and a detection method thereof, which have the following beneficial effects:

[0021] (1) In the present invention, a constant temperature box is designed to match multiple carrier boxes to form a corresponding heat preservation space, so as to facilitate the heat preservation operation of multiple samples in the multiple carrier boxes. Since multiple samples can be kept warm at the same time, the detection efficiency is higher. Through the design of multiple carrier boxes, relatively independent reaction spaces are formed for multiple samples, and while ensuring basic endotoxin detection, endotoxin detection operations for multiple samples can be performed successively.

[0022] (2) In the present invention, the water tank is divided into a plurality of corresponding inlets by the provision of a partitioned isolation structure. On the one hand, it is convenient for the corresponding placement of a plurality of matching bottom shells. On the other hand, it is also convenient for the follow-up closure of the water tank after the bottom shell is lifted, thereby reducing the temperature loss in the water tank and lowering energy consumption.

[0023] (3) In the present invention, through the design of the automatic lifting structure, the matching carrier box forms a relative position adjustment between the self-loading and placing port and the corresponding placement port, so as to facilitate the operation of taking and placing samples in the carrier box, and the degree of automation is relatively high.

[0024] (4) In the present invention, by designing the cut-in follower structure, the connection and adjustment between the top cover and the curved door at the access opening can be realized, thereby facilitating the synchronous opening of the top cover and the curved door, making it more convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the present invention;

[0026] Figure 2 For the present invention Figure 1 Schematic diagram of the local enlarged structure at A in the middle;

[0027] Figure 3 This is a schematic diagram of the three-dimensional structure of the fixed strip, the partition plate and the rotating isolation plate of the present invention;

[0028] Figure 4 For the present invention Figure 3 Schematic diagram of the local enlarged structure at B in the middle;

[0029] Figure 5 It is a partially exploded three-dimensional structural diagram of the hook cylinder, track member and lifting hook of the present invention;

[0030] Figure 6 A schematic diagram of the three-dimensional structure of the present invention from another angle;

[0031] Figure 7 For the present invention Figure 6 Schematic diagram of the local enlarged structure at C in the middle;

[0032] Figure 8 It is a schematic diagram of a three-dimensional structure of a stepped cross-section of the curved door, the inner slide, the rotating hook rod, etc. according to the present invention;

[0033] Figure 9 It is a schematic diagram of the three-dimensional structure of the internal slide, elastic spring and screw drive rod of the present invention;

[0034] Figure 10 It is a partially cutaway three-dimensional structural diagram of the swing sleeve, track ring sleeve and telescopic strip of the present invention;

[0035] Figure 11 Schematic diagram of the exploded three-dimensional structure of the bottom shell, top cover and embedded fixing frame of the present invention;

[0036] Figure 12 It is a schematic diagram of a cross-sectional three-dimensional structure of the water storage tank, the covering pipe and the water inlet pipe of the present invention;

[0037] Figure 13 It is a partially cutaway perspective structural diagram of the present invention showing a state where the rotating hook rod is rotating relative to the top cover;

[0038] Figure 14 It is a schematic diagram of a three-dimensional structure of the bent plate door, the rotating hook rod and the extended operating rod in a stepped cross-section and viewed from below according to the present invention;

[0039] Figure 15 It is a schematic diagram of the exploded three-dimensional structure of the through plate, the swing sleeve and the track ring sleeve of the present invention;

[0040] Figure 16 A bottom-view schematic diagram of the three-dimensional structure of the fixed strip, the partition plate, the rotating isolation plate, etc. of the present invention;

[0041] Figure 17 Schematic diagram of the arrangement order of the test tubes of the present invention placed on the fixed rack.

[0042] In the figure: 1. Base; 2. Water storage tank; 3. Insulation cover; 4. Cover pipe; 5. Water inlet pipe; 6. Return pipe; 7. Circulation pump; 8. Heater; 9. Bottom shell; 10. Top cover; 11. Insertion bracket; 12. Bent door; 13. Insertion port; 14. Side bracket; 15. Fixing strip; 16. Divider plate; 17. Rotating isolation plate; 18. Return spring; 19. External hand ring; 20. Internal slide; 21. Rotating hook rod; 22. Mounting cavity; 23. Track groove; 24. Elastic spring; 25. Rotating cylinder; 26. Screw drive rod; 27. Through plate; 28. Swing sleeve; 29. ​​Track Ring sleeve; 30. Telescopic strip; 31. Connecting spring; 32. Transmission rod; 33. Extended operating rod; 34. First transfer link; 35. Limit spring; 36. Second transfer link; 37. Servo motor; 38. Lead screw; 39. Optical bar; 40. Wire sleeve; 41. Moving frame; 42. Lifting frame; 43. External pressure sensor; 44. Hook cylinder; 45. Track member; 46. Permanent magnet; 47. Electromagnet; 48. Lifting hook; 49. Built-in pressure sensor; 50. Auxiliary spring; 51. Auxiliary push strip; 52. Upper gradient slope; 53. Lower gradient slope; 54. Limit surface; 55. Holding ball. DETAILED DESCRIPTION

[0043] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0044] For examples, see Figures 1-17, an automated endotoxin detection device includes a base 1, a constant temperature box and a plurality of carrier boxes, a mounting strip is opened on the base 1, two covering pipes 4 are arranged in the constant temperature box, the constant temperature box includes a water reservoir 2 and a thermal insulation cover 3, the water reservoir 2 is fixedly connected in the mounting strip, the thermal insulation cover 3 is fixedly connected to the base 1, and a contact seal is provided between the thermal insulation cover 3 and the water reservoir 2, the two covering pipes 4 are both arranged in the water reservoir 2, and the two covering pipes 4 are respectively connected with a water inlet pipe 5 and a return pipe 6, a circulating pump 7 and a heater 8 are installed in the base 1, the water inlet pipe 5 and the return pipe 6 are respectively connected with the pump inlet and the pump outlet of the circulating pump 7, which can realize the circulation drive of the temperature transfer water in the constant temperature box, and the heater 8 is equipped with the water inlet pipe 5 to form a covering heating, and the constant The design of the incubator is equipped with multiple carrier boxes to form a space corresponding to the water bath insulation, so as to facilitate the water bath insulation operation of multiple samples in the multiple carrier boxes. Since multiple samples can be kept warm at the same time, the detection efficiency is higher. A partition isolation structure is installed in the constant temperature box. The partition isolation structure divides the constant temperature box into multiple insertion ports, providing the necessary insertion space for multiple bottom shells 9. The partition isolation structure includes a fixing bar 15, which is fixedly connected to the water reservoir 2. The fixing bar 15 is fixedly connected to multiple partition plates 16. The multiple partition plates 16 are all fixedly connected to the water reservoir 2. The height of the multiple partition plates 16 is less than the depth of the water reservoir 2. The top ends of the multiple partition plates 16 are flush with the top ends of the water reservoir 2, so that the bottom ends of the partition plates 16 are flush with the top ends of the water reservoir 2. There is a space between the inner bottom walls of the water tank 2 through which the warm water can flow. The inlet is the area between two adjacent partition plates 16. A rotating isolation plate 17 is rotatably connected between each two adjacent partition plates 16. The bottom ends of multiple rotating isolation plates 17 are fixedly connected to return springs 18. Multiple return springs 18 are fixedly connected to the water tank 2. Through the provision of a partitioned isolation structure, the water tank 2 is divided into multiple inlets. On the one hand, it is convenient for the corresponding placement of multiple bottom shells 9. On the other hand, it is also convenient for the follow-up closure of the water tank 2 after the bottom shell 9 is lifted, thereby reducing the temperature loss in the water tank 2 and consuming less energy. A limiting surface 54 matching the rotating isolation plate 17 is provided on the fixing bar 15. When the bottom shell 9 is lifted out of the water tank 2, When the downward pressure on the rotating isolation plate 17 fails, the rotating isolation plate 17 rotates and rises in the water tank 2 under the elastic reset action of the reset spring 18, and the limiting surface 54 forms a limit for the rotating isolation plate 17 after rotation and rising, so as to ensure that the rotating isolation plate 17 is just covered and closed when it is rotated and raised. The multiple carrier boxes include a bottom shell 9 and a top cover 10, and the multiple top covers 10 are respectively provided with an insertion contact seal between the multiple bottom shells 9, and the multiple bottom shells 9 are respectively provided with an insertion fixing frame 11. Through the design of multiple carrier boxes, relatively independent reaction spaces for multiple samples are formed, which can form endotoxin detection operations of multiple samples in succession while ensuring basic endotoxin detection, and the multiple bottom shells 9 are matched with the multiple insertion ports.

[0045] It should be further explained that the thermostat is provided with a take-out port, which is provided on the insulation cover 3, and a curved plate door 12 is rotatably connected in the take-out port to form a necessary closure for the take-out port. A cut-in follow-up structure is installed on the curved plate door 12, and multiple top covers 10 are provided with insertion ports 13 that match the cut-in follow-up structure. The cut-in follow-up structure includes an external hand ring 19, an internal slide 20 and a rotating hook rod 21. The external hand ring 19 is fixedly connected to the curved plate door 12, and an installation cavity 22 is provided on the curved plate door 12. A track groove 23 is provided in the installation cavity 22, and the internal slide 20 slides The inner slide 20 is fixedly connected to the track groove 23, and the track groove 23 is fixedly connected with an elastic spring 24, the elastic spring 24 is fixedly connected to the internal slide 20, the rotating hook rod 21 is fixedly connected with a rotating cylinder 25, the rotating cylinder 25 is rotatably connected to the internal slide 20, the rotating cylinder 25 is located in the installation cavity 22, the internal slide 20 is slidably connected with a screw drive rod 26, a through plate 27 is provided in the screw drive rod 26, the through plate 27 is fixedly connected to the rotating cylinder 25, and a progressive drive mechanism is installed in the curved door 12. The progressive drive mechanism is used to drive the screw drive rod 26. By cutting in, the progressive drive mechanism is rotated. The design of the dynamic structure can realize the connection and adjustment of the top cover 10 and the curved door 12 at the access port, thereby facilitating the synchronous opening of the top cover 10 and the curved door 12, making it more convenient to use. The progressive drive mechanism includes a swing sleeve 28 and a track ring sleeve 29. The swing sleeve 28 is rotatably connected to the curved door 12. A telescopic bar 30 is slidably connected to the swing sleeve 28. The telescopic bar 30 is fixedly connected to a connecting spring 31. The connecting spring 31 is fixedly connected to the swing sleeve 28. The telescopic bar 30 is fixedly connected to a transmission rod 32. The transmission rod 32 is inserted into the track ring sleeve 29. The track ring sleeve 29 is connected to the internal The slide 20 is fixedly connected, the swing sleeve 28 is fixedly connected to the outward operating rod 33, the outward operating rod 33 is fixedly connected to a first rotating link 34, the first rotating link 34 is fixedly connected to a limit spring 35, the limit spring 35 is fixedly connected to a second rotating link 36, the second rotating link 36 is rotatably connected to the external wristband 19, to realize the auxiliary limit after the rotating hook rod 21 is turned up and raised, and can also control the auxiliary limit after the rotating hook rod 21 is lowered and turned down. The front end of the outward operating rod 33 is rotatably connected to a holding ball 55, which is convenient for auxiliary gripping when the outward operating rod 33 applies force.

[0046] It should be further explained that an automatic lifting structure is provided in the constant temperature box, and the automatic lifting structure is used for the movement and adjustment of multiple bottom shells 9. Two side brackets 14 are fixedly connected to the base 1, and the two side brackets 14 are used for the bracket installation of the automatic lifting structure. The automatic lifting structure includes a servo motor 37, a screw 38 and a plurality of light bars 39. The screw 38 is rotatably connected between the two side brackets 14, and the plurality of light bars 39 are fixedly connected between the two side brackets 14. A through hole corresponding to the screw 38 and the plurality of light bars 39 is provided on the thermal insulation cover 3. The servo motor 37 is installed on one of the two side brackets 14, and the servo The output shaft of the motor 37 is fixedly connected to the lead screw 38, and a wire sleeve 40 is threadedly connected to the lead screw 38. The wire sleeve 40 is fixedly connected to a mobile frame 41 outside. A plurality of light holes are opened on the mobile frame 41, and the plurality of light holes are matched with a plurality of light bars 39 respectively. The mobile frame 41 is slidably connected to a lifting frame 42, and the mobile frame 41 is equipped with an external pressure sensor 43. The top of the lifting frame 42 is provided with a plane matching the external pressure sensor 43. A magnetic drive component is installed between the lifting frame 42 and the mobile frame 41. The lifting frame 42 is fixedly connected to a hook cylinder 44, and the hook cylinder 44 is equipped with a plurality of track parts 45. The plurality of track parts 45 are respectively connected to a plurality of The bottom shell 9 is fixedly connected, and two pressure alignment components are installed on the lifting frame 42. The magnetic drive component includes a permanent magnet 46 and a plurality of electromagnets 47. A mounting slot is provided on the lifting frame 42. The permanent magnet 46 is fixedly connected in the mounting slot. The plurality of electromagnets 47 are fixedly connected to the movable frame 41. The plurality of electromagnets 47 are matched with the permanent magnet 46. The pressure alignment component includes two lifting hooks 48 and two built-in pressure sensors 49. The two built-in pressure sensors 49 are both installed on the lifting frame 42. The two lifting hooks 48 are both slidably connected to the lifting frame 42. The two lifting hooks 48 are fixedly connected to the lifting frame 42. The two auxiliary springs 50 are fixedly connected to the two lifting hooks 48. The two auxiliary springs 50 are fixedly connected to the two lifting hooks 48. 0 are fixedly connected with auxiliary push bars 51, and the two auxiliary push bars 51 are matched with two built-in pressure sensors 49 respectively. The two auxiliary push bars 51 are slidably matched with the lifting frame 42. The top of the track part 45 is provided with two upper gradual slopes 52, and the two upper gradual slopes 52 are matched with the two lifting hooks 48 respectively. The bottom end of the track part 45 is provided with two lower gradual slopes 53, and the two lower gradual slopes 53 are matched with the two ends of the bar hook cylinder 44 respectively. Through the design of the automatic lifting structure, the relative position movement and adjustment between the self-loading and placing port and the corresponding insertion port of the matching carrier box are formed to facilitate the taking and placing operations of the samples in the carrier box, and the degree of automation is high.

[0047] The servo motor 37, electromagnet 47, circulation pump 7, heater 8, external pressure sensor 43 and built-in pressure sensor 49 in this embodiment are all conventional devices purchased on the market and well known to those skilled in the art. In the present invention, we only use them and do not improve their structure and function. For those skilled in the art, their setting method, installation method and electrical connection method only need to be debugged according to the requirements of the instruction manual, and will not be described in detail here.

[0048] In summary, the working principle of the automatic endotoxin detection device and the detection method thereof is as follows: before use, a control circuit is installed for the automatic endotoxin detection device, and warm water is pre-installed in the water tank 2. The circulation pump 7 is powered on to realize the external suction of the warm water in the water tank 2 through the return pipe 6, and the sucked warm water is pumped back into the water tank 2 through the water inlet pipe 5. When the warm water passes through the water inlet pipe 5, it will be heated by the heater 8 to form a temperature-raising treatment of the warm water to ensure that the temperature of the warm water in the water tank 2 is maintained. During use, the automatic lifting structure can realize the lifting and moving of a single bottom shell 9 among multiple bottom shells 9, so that The bottom shell 9 can be adjusted to move between the insertion port and the take-out port. When the sample needs to be loaded into the carrier box, the carrier box is transferred to the take-out port by the automatic lifting structure. The top cover 10 located at the take-out port is connected to the curved plate door 12 by adjusting the cut-in follower structure. Then, in the process of opening the curved plate door 12, the top cover 10 can be opened synchronously with the opening of the curved plate door 12. After opening, the test tube pre-loaded with the sample and the horseshoe crab reagent is inserted into the fixed rack 11. In order to facilitate the synchronous loading of multiple test tubes, the fixed rack 11 and the bottom shell 9 adopt a quickly detachable structure, which enables multiple test tubes to be pre-inserted and placed in the outside world. The fixing frame 11 is installed relative to the bottom shell 9 to achieve the synchronous and rapid loading of multiple test tubes relative to the bottom shell 9. After the test tubes are loaded, the curved plate door 12 is closed relative to the take-out port, so that the top cover 10 and the corresponding bottom shell 9 are synchronously closed, and the top cover 10 and the curved plate door 12 are separated by adjusting the cut-in follower structure to separate them. After that, the automatic lifting structure continues to work to control the bottom shell 9 and the top cover 10 to form a carrier box that is matched with the bottom shell 9 and the top cover 10 to reset and move the entire carrier box from the take-out port to the corresponding insertion port. When the bottom shell 9 is placed in the insertion port, the bottom of the bottom shell 9 contacts the temperature transfer water in the water storage tank 2, and the bottom shell 9 is formed under the action of heat conduction. The reaction heating of the sample and the horseshoe crab reagent is completed in the order of the above steps. Multiple batches of samples are loaded into multiple carrier boxes respectively. When the insulation reaction of the sample in the carrier box is completed, the corresponding carrier box is transferred from the entrance to the take-out port again through the automatic lifting structure, and finally the sample and horseshoe crab reagent after the reaction are taken out. The sample and horseshoe crab reagent after the reaction in the test tube are compared and judged to form an endotoxin detection. In order to facilitate the observation of the reaction status of the test tube in the carrier box and the recording of the reaction process, the insulation cover 3 and multiple top covers 10 are made of transparent material, and multiple cameras are installed in the insulation cover 3 to form a camera record of the inside of multiple carrier boxes.

[0049] Furthermore, the operating principle of the automatic lifting structure is that the servo motor 37 is powered on to realize the rotation adjustment of the screw 38 relative to the two side brackets 14. Under the cooperation of the screw 38 and the wire sleeve 40 and the guidance of the optical rod 39 on the movable frame 41, the rotating screw 38 can realize the position movement of the movable frame 41 along the optical rod 39. Since the lifting frame 42 and the movable frame 41 are connected in an up and down sliding manner, the lifting frame 42 will move synchronously with the position movement of the movable frame 41 along the optical rod 39. When the multiple electromagnets 47 are not powered on to generate an electromagnetic field, under the action of the carrier box's own gravity, the carrier box falls into the corresponding insertion port. Since the track member 45 is fixedly connected to the bottom shell 9, the track member 45 also moves when the bottom shell 9 falls into the insertion port. The plurality of bottom shells 9 fall into the plurality of insertion openings respectively, and the plurality of track members 45 are also at the same height with a similar height. Due to the gravity of the lifting frame 42 itself, it will also fall relative to the moving frame 41, and the plurality of track members 45 are all in sliding cooperation with the hook cylinder 44. Therefore, when the plurality of electromagnets 47 do not generate an electromagnetic field, the hook cylinder 44 can form a sliding switching cooperation relative to the plurality of track members 45. The two lifting hooks 48 are pre-adjusted to enter the upper gradient slope 52 at both ends of the same track member 45 respectively. By comparing and observing the readings of the two built-in pressure sensors 49, due to the action of the two auxiliary springs 50, the two lifting hooks 48 will form contact and fit relative to the two upper gradient slopes 52 respectively, and the two lifting hooks 48 are kept in contact. The contact and fitting state of the upper gradual slope 52 at both ends of the same track member 45 is respectively determined, and the relative position of the two lifting hooks 48 relative to the track member 45 is fine-tuned until the two built-in pressure sensors 49 have similar readings within a certain range, and the range value is set. Thereafter, as long as the two built-in pressure sensors 49 are within the range value, the position of the two lifting hooks 48 can be determined. At this position, the lower hook cylinder 44 has complete coverage relative to the single track member 45 therein and does not form a connection with other track members 45. In this state, multiple electromagnets 47 are correspondingly energized. Under the relative cooperation of the electromagnets 47 and the permanent magnets 46, the relative position of the lifting frame 42 relative to the moving frame 41 can be raised. In order to ensure the raising of the lifting frame 42 The N pole and S pole of the permanent magnet 46 are arranged opposite to each other in an upper and lower manner, and multiple electromagnets 47 are energized in sequence from bottom to top to form an electromagnetic field acting on the permanent magnet 46. The magnetic pole directions of the electromagnets 47 are controlled so that the electromagnets 47 on the upper side of the permanent magnet 46 generate a mutual attraction force on the permanent magnet 46, while the electromagnets 47 on the lower side of the permanent magnet 46 generate a mutual repulsion force on the permanent magnet 46, so that the lifting frame 42 has the force to pull up and push down, ensuring that the lifting frame 42 is raised relative to the moving frame 41. When the plane on the lifting frame 42 contacts the external pressure sensor 43 and the external pressure sensor 43 has a pressure reading, it means that the lifting frame 42 has been raised to the position. In this state, the bottom shell 9 connected to the track member 45 located in the hook cylinder 44 is lifted synchronously.There is a safe height distance between the bottom end of the lifted bottom shell 9 and the other top covers 10 that have not been lifted. After that, the servo motor 37 is running, which can realize the movement of the lifted bottom shell 9 in the thermal insulation cover 3, making it convenient for the lifted bottom shell 9 to enter and adjust relative to the access opening.

[0050] Furthermore, when the bottom shell 9 enters the access port, the top cover 10 on the bottom shell 9 also enters the access port. By controlling the number of revolutions of the servo motor 37, the bottom shell 9 is completely aligned with the access port. Then, the servo motor 37 is controlled to stop. Figure 2As shown, under normal conditions, the swing sleeve 28 is in a relatively raised state relative to the exposed end of the curved door 12. In this state, the limit spring 35 can limit the swing sleeve 28. In this state, the screw drive rod 26 is in a certain relatively pulled-out state relative to the rotating cylinder 25, and the rotating hook rod 21 rotates and falls completely into the installation cavity 22. On the basis of the above-mentioned state, the operator holds the holding ball 55 with the palm of his hand and applies force to the outward operating rod 33 through the holding ball 55, so that the outward operating rod 33 is rotated and adjusted relative to the curved door 12 to control the rotation and lifting of the telescopic bar 30. Due to the action of the connecting spring 31 and the elastic spring 24, the screw drive rod 26 will be raised relative to the exposed end of the telescopic bar 30 in the early stage of rotation and lifting. The sliding drive of the internal slide 20, that is, the screw drive rod 26 is slidably inserted relative to the rotating cylinder 25. Under the transmission action of the through plate 27 and the screw drive rod 26, during the insertion of the screw drive rod 26 relative to the rotating cylinder 25, the rotating cylinder 25 will be rotated relative to the internal slide 20, and finally the lifting drive of the rotating hook rod 21 is achieved. The rotating hook rod 21 that is rotated and raised will be inserted relative to the insertion port 13, and when the rotating hook rod 21 rotates into the extreme position of the insertion port 13, the rotating hook rod 21 will not rotate and rise, but further rotate to press the swing sleeve 28, and under the transmission action of the telescopic bar 30, the transmission rod 32 will be further raised. Since the track ring sleeve 29 is fixed to the screw drive rod 26 The inner slide 20 is raised along the track groove 23, and the telescopic bar 30 overcomes the elastic force of the connecting spring 31 and is further inserted relative to the swing sleeve 28. The inner slide 20 is raised through the rotating cylinder 25, which drives the rotating hook rod 21 to rise. The raised rotating hook rod 21 drives the top cover 10 connected thereto to rise synchronously until the top cover 10 and the inner top wall of the curved door 12 are tightly fitted to each other and limited. The top cover 10 cannot be further raised, and the rotating hook rod 21 cannot be further raised. During this process, The raised top cover 10 will be pulled out relative to the bottom shell 9 into which it was originally inserted, so as to avoid mutual interference between the top cover 10 and the originally inserted bottom shell 9 during the subsequent synchronous opening process of the curved door 12. In this state, the rotation angle of the swing sleeve 28 will cause the limit spring 35 to pass through the extreme compression state from the elongated state and then enter the elongated state again. The limit spring 35 in this state can form an auxiliary limit for the telescopic strip 30 that is rotated and raised after it is raised, so as to avoid failure of the connection limit between the rotating hook rod 21 and the top cover 10. In this state, the curved door 12 can be opened by applying a rotational opening force to the curved door 12 through the external hand ring 19, and the top cover 10 with a fixed limit in the curved door 12 will also be opened synchronously.

[0051] Continuing further, after the operation is completed, the curved door 12 is closed first and then the extended operating rod 33 is lifted, so that the top cover 10 can be inserted and closed relative to the bottom shell 9. Due to the elastic reset effect of the elastic spring 24, the top cover 10 will first fall and be inserted relative to the bottom shell 9, and then the rotating hook rod 21 will be rotated out and escaped relative to the insertion port 13 on the top cover 10, and in this process, the limit spring 35 will pass through the extreme compression state again and enter the extension state, so that the telescopic bar 30 is rotated and lowered to the limit position, that is, the rotating hook rod 21 is received into the installation cavity 22. After that, the servo motor 37 is operated to realize the reset of the base 1 relative to the original corresponding insertion entrance, and finally the multiple electromagnets 47 are sequentially powered off from top to bottom to realize the falling reset of the lifting frame 42 relative to the moving frame 41, so as to ensure that the bottom shell 9 falls smoothly into the installation cavity. In the entrance, after the bottom shell 9 falls into the entrance, the rotating isolation plate 17 will be driven to rotate downward, so the bottom end of the bottom shell 9 will be in contact with the temperature transfer water in the water tank 2 to ensure effective heat conduction. The covering pipe 4 connected to the water inlet pipe 5 is located on the side where the rotating isolation plate 17 falls. The temperature transfer water entering the water tank 2 through the covering pipe 4 will be guided by the rotating isolation plate 17 in an inclined state, so that the temperature transfer water after heating can better form contact with the bottom shell 9. Considering the influence of the middle part of the covering pipe 4 and the length of the water channels at both ends, the diameter of the flow hole on the covering pipe 4 adopts a structure that gradually becomes smaller from both ends to the middle. When the test tube needs to be oscillated, the test tube is repeatedly raised and lowered by controlling one or two electromagnets 47 located at the bottom to be repeatedly energized and de-energized, thereby achieving the purpose of test tube oscillation.

[0052] The sample and lysate in the sample and lysate tubes are prepared as follows:

[0053] λ is the labeled sensitivity (EU / ml) of the limulus amebocyte lysate in the gel-based assay. It is a known number. Due to different purposes of use, manufacturers have multiple specifications of labeled sensitivity, such as:

[0054] 0.03EU / ml, 0.06EU / ml, 0.125EU / ml, 0.25EU / ml, 0.5EU / ml, 1.0EU / ml, 2.0EU / ml.

[0055] During use, the sensitivity of the limulus amebocyte lysate purchased should be indicated.

[0056] The sensitivity is not specified here. Instead, it is represented by λ (an industry-wide sensitivity symbol). This can be considered a known value and does not affect the calculation.

[0057] Reagent preparation (prepared using endotoxin working standard)

[0058]

[0059] Experimental procedures

[0060] Reaction tube preparation (the order of arrangement of the test tubes on the fixed rack 11 is as shown in the attached Figure 17 shown)

[0061]

[0062] Dissolution of the limulus amebocyte lysate: Add water for bacterial endotoxin testing to the limulus amebocyte lysate according to the labeled amount (specifications of the purchased reagent), and shake gently to completely dissolve the limulus amebocyte lysate. Be careful not to cause bubbles. The dissolved reagent should be used within 10 minutes (ready to use).

[0063] The reaction must be carried out with 0.1ml / test tube of Limulus Amebocyte Lysate (LAL) reagent (marked in red in the corresponding table). Any excess should be dispensed into 0.1ml / EP test tube.

[0064] Reaction on the machine

[0065] Seal the mouth of the test tube and place it in 37°C warm water in the water reservoir and incubate for 60 minutes ± 2 minutes. The test tube should be oscillated at the initial stage when the base is reset relative to the inlet to facilitate the shaking of the sample and limulus amebocyte lysate in the test tube. Vibration should be avoided during the subsequent incubation period.

[0066] The basis for result determination and observation is as follows:

[0067] Gently remove the test tube from the holder 11 and slowly invert it 180°. If the reaction between the sample and the limulus amebocyte lysate in the test tube forms a solid gel that does not deform or slip from the wall of the test tube, it is positive and recorded as (+).

[0068] No gel is formed or a gel is formed but cannot remain intact and slides off the wall of the test tube, which is negative and recorded as (-).

[0069] Interpretation of the results

[0070] 1. The test is valid only when the results of the negative control test tube are all negative, and the results of the positive control test tube and the test sample positive control test tube are all positive. Otherwise, it is invalid.

[0071] 2. If the negative control test tube is positive, it indicates that the limulus amebocyte lysate, the water used for bacterial endotoxin testing, or the laboratory equipment may be contaminated. If the positive control test tube is negative, it indicates that the limulus amebocyte lysate activity has been lost, the potency of the endotoxin standard solution has been reduced, the sensitivity of the limulus amebocyte lysate or the endotoxin potency label is inaccurate, or the endotoxin standard solution has been incorrectly diluted.

[0072] 3. If the positive control test tube of the test sample is negative, it indicates that there are interfering factors in the reaction system.

[0073] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An automated endotoxin detection device, comprising a base (1), characterized in that: The invention also includes a thermostat and a plurality of carrier boxes. The base (1) is provided with a mounting strip. Two covering pipes (4) are provided in the thermostat. The two covering pipes (4) are respectively connected to a water inlet pipe (5) and a water return pipe (6). A circulation pump (7) and a heater (8) are installed in the base (1). The water inlet pipe (5) and the water return pipe (6) are respectively connected to the pump inlet and the pump outlet of the circulation pump (7). The heater (8) is matched with the water inlet pipe (5) to form a covering heating. A partition isolation structure is installed in the thermostat. The partition isolation structure divides the thermostat into a plurality of inlets. The plurality of carrier boxes Each comprises a bottom shell (9) and a top cover (10), and an insertion contact seal is provided between each of the top covers (10) and the bottom shells (9), and an insertion fixing frame (11) is provided in each of the bottom shells (9). Each of the bottom shells (9) matches the insertion ports, and a take-out port is provided on the thermostat, and a curved plate door (12) is rotatably connected in the take-out port, and a cut-in follow-up structure is installed on the curved plate door (12). Each of the top covers (10) is provided with an insertion port (13) matching the cut-in follow-up structure, and an automatic lifting structure is provided in the thermostat, and the automatic lifting structure is used for the plurality of bottom shells. (9) is movable and adjusted, two side brackets (14) are fixedly connected to the base (1), and the two side brackets (14) are used for the bracket installation of the automatic lifting structure. The cut-in follow-up structure includes an external hand ring (19), an internal slide (20) and a rotating hook rod (21). The external hand ring (19) is fixedly connected to the curved plate door (12), and a mounting cavity (22) is provided on the curved plate door (12). A track groove (23) is provided in the mounting cavity (22). The internal slide (20) is slidably connected in the track groove (23), and an elastic spring (21) is fixedly connected in the track groove (23). 4), the elastic spring (24) is fixedly connected to the internal slide (20), the rotating hook rod (21) is fixedly connected to a rotating cylinder (25), the rotating cylinder (25) is rotatably connected to the internal slide (20), the rotating cylinder (25) is located in the installation cavity (22), the internal slide (20) is slidably connected to a screw drive rod (26), a through plate (27) is provided in the screw drive rod (26), the through plate (27) is fixedly connected to the rotating cylinder (25), a progressive drive mechanism is installed in the curved plate door (12), and the progressive drive mechanism is used to drive the screw drive rod (26); The automatic lifting structure comprises a servo motor (37), a lead screw (38) and a plurality of optical rods (39), wherein the lead screw (38) is rotatably connected between the two side brackets (14), and the plurality of optical rods (39) are fixedly connected between the two side brackets (14). The servo motor (37) is mounted on one of the two side brackets (14), and the output shaft of the servo motor (37) is fixedly connected to the lead screw (38). A threaded sleeve (40) is threadedly connected to the lead screw (38), and the threaded sleeve (40) is fixedly connected to the outside. The movable frame (41) is connected to the movable frame (41), and a plurality of light holes are opened on the movable frame (41), and the plurality of light holes are matched with a plurality of light bars (39) respectively. The movable frame (41) is slidably connected to the lifting frame (42), and the movable frame (41) is installed with an external pressure sensor (43). The top of the lifting frame (42) is provided with a plane matching the external pressure sensor (43). A magnetic drive component is installed between the lifting frame (42) and the movable frame (41). The lifting frame (42) is fixedly connected to a hook cylinder (44), and the hook cylinder (44) is equipped with A plurality of track members (45), wherein the plurality of track members (45) are respectively fixedly connected to the plurality of bottom shells (9), two pressure alignment components are installed on the lifting frame (42), the magnetic drive component comprises a permanent magnet (46) and a plurality of electromagnets (47), the lifting frame (42) is provided with a mounting groove, the permanent magnet (46) is fixedly connected in the mounting groove, the plurality of electromagnets (47) are all fixedly connected to the moving frame (41), the plurality of electromagnets (47) are all matched with the permanent magnet (46), and the pressure alignment component comprises The invention comprises two lifting hooks (48) and two built-in pressure sensors (49), the two built-in pressure sensors (49) are both mounted on the lifting frame (42), the two lifting hooks (48) are both slidably connected to the lifting frame (42), the two lifting hooks (48) are both fixedly connected with auxiliary springs (50), the two auxiliary springs (50) are both fixedly connected with auxiliary push bars (51), the two auxiliary push bars (51) are respectively matched with the two built-in pressure sensors (49), and the two auxiliary push bars (51) are both slidably matched with the lifting frame (42).

2. An automated endotoxin detection device according to claim 1, characterized in that: The thermostatic box comprises a water tank (2) and a heat-insulating cover (3); the water tank (2) is fixedly connected to the mounting strip; the heat-insulating cover (3) is fixedly connected to the base (1); a contact seal is provided between the heat-insulating cover (3) and the water tank (2); the two covering pipes (4) are both provided in the water tank (2); and the access opening is provided on the heat-insulating cover (3).

3. An automated endotoxin detection device according to claim 2, characterized in that: The partition isolation structure comprises a fixing bar (15), the fixing bar (15) being fixedly connected in the water tank (2), the fixing bar (15) being fixedly connected to a plurality of partition plates (16), the plurality of partition plates (16) being fixedly connected in the water tank (2), the heights of the plurality of partition plates (16) being less than the depth of the water tank (2), the tops of the plurality of partition plates (16) being flush with the top of the water tank (2), the insertion port being the area between two adjacent partition plates (16), a rotating isolation plate (17) being rotatably connected between each two adjacent partition plates (16), the bottom ends of the plurality of rotating isolation plates (17) being fixedly connected to a return spring (18), and the plurality of return springs (18) being fixedly connected in the water tank (2).

4. An automated endotoxin detection device according to claim 3, characterized in that: The progressive driving mechanism comprises a swing sleeve (28) and a track ring sleeve (29), wherein the swing sleeve (28) is rotatably connected to the curved plate door (12), a telescopic bar (30) is slidably connected to the swing sleeve (28), the telescopic bar (30) is fixedly connected to a connecting spring (31), the connecting spring (31) is fixedly connected to the swing sleeve (28), the telescopic bar (30) is fixedly connected to a transmission rod (32), the transmission rod (32) is inserted into the track ring sleeve (29), the track ring sleeve (29) is fixedly connected to the internal slide (20), the swing sleeve (28) is fixedly connected to an outward-extending operating rod (33), the outward-extending operating rod (33) is fixedly connected to a first transfer link (34), the first transfer link (34) is fixedly connected to a limit spring (35), the limit spring (35) is fixedly connected to a second transfer link (36), and the second transfer link (36) is rotatably connected to the external hand ring (19).

5. An automated endotoxin detection device according to claim 4, characterized in that: The top of the track member (45) is provided with two upper gradual slopes (52), and the two upper gradual slopes (52) are matched with the two lifting hooks (48) respectively. The bottom of the track member (45) is provided with two lower gradual slopes (53), and the two lower gradual slopes (53) are matched with the two ends of the bar hook cylinder (44) respectively. The fixed bar (15) is provided with a limiting surface (54) that matches the rotating isolation plate (17). The front end of the extended operating rod (33) is rotatably connected to a holding ball (55).

6. A detection method for an automated endotoxin detection device, characterized in that: An automated endotoxin detection device according to any one of claims 1 to 5 is used, comprising the following steps: S1. Before use, a control circuit is installed for the automatic endotoxin detection device, and warm water is pre-installed in the water tank (2). The circulating pump (7) is powered on to suck the warm water out of the water tank (2) through the return pipe (6), and the sucked warm water is pumped back into the water tank (2) through the water inlet pipe (5). When the warm water passes through the water inlet pipe (5), it is heated by the heater (8) to form a temperature-raising treatment of the warm water, so as to ensure that the temperature of the warm water in the water tank (2) is maintained; S2. During use, the automatic lifting structure can realize the lifting and moving operation of a single bottom shell (9) among the multiple bottom shells (9), so that the bottom shell (9) can be adjusted to move its position between the insertion port and the take-out port. When it is necessary to load a sample into the carrier box, the automatic lifting structure is used to transfer the carrier box to the take-out port. By adjusting the cut-in follower structure, the top cover (10) located at the take-out port and the curved plate door (12) are connected to each other inside and outside. Then, in the process of opening the curved plate door (12), the top cover (10) can be opened synchronously with the opening of the curved plate door (12). After opening, the test tube pre-loaded with the sample and the horseshoe crab reagent is inserted into the fixed frame (11); S3. After the test tube is loaded, the curved plate door (12) is closed relative to the take-out port, so that the top cover (10) and the corresponding bottom shell (9) are synchronously closed, and the top cover (10) and the curved plate door (12) fixed to each other are reset by adjusting the cut-in follower structure, so that the top cover (10) and the curved plate door (12) are separated. Thereafter, the automatic lifting structure continues to work to control the bottom shell (9) and the top cover (10) to be matched with each other to reset and move the entire carrier box from the take-out port to the corresponding insertion port. When the bottom shell (9) is placed in the insertion port, the bottom of the bottom shell (9) contacts the warm water in the water storage tank (2), and the reaction heating of the sample and the horseshoe crab reagent in the bottom shell (9) is formed under the action of heat conduction; S4. Complete the loading of multiple batches of samples into multiple carrier boxes in sequence according to the above steps. When the sample insulation reaction in the carrier box is completed, the corresponding carrier box is transferred from the placement entrance to the placement entrance again through the automatic lifting structure, and finally the samples and horseshoe crab reagents after the reaction are completed are taken out, and the samples and horseshoe crab reagents after the reaction in the test tube are compared and judged to form an endotoxin detection.

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