Oral liquid containing dandelion extract

Abstract

The invention belongs to the technical field of traditional Chinese medicine, and discloses oral liquid containing dandelion extract. The oral liquid comprises a dandelion extract, a pubescent holly root extract, a fructus forsythiae extract, a stephania japonica extract, a platycodon grandiflorum extract, a honeysuckle extract, a burdock extract, a houttuynia cordata extract, a turmeric extract, a liquorice extract, a spirulina extract and 3-5 parts of glucose. The dandelion extract rich in dandelion peptide, the pubescent holly root extract, the fructus forsythiae extract, the Japanese stephania root extract, the platycodon root extract, the honeysuckle flower extract, the burdock fruit extract, the heartleaf houttuynia herb extract, the turmeric extract and the liquorice root extract are simultaneously prepared by using a unique process; the oral liquid prepared by compounding the dandelion extract and other natural components has antibacterial and antiviral effects and can be used for improving immunity.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to an oral liquid containing dandelion extract. Background Art

[0002] Dandelion, also known as dandelion, grandma's grass or yellow flowered dandelion, is a perennial herb with a long flowering period. It is usually harvested in summer and autumn. The whole plant can be used as medicine. It tastes bitter and sweet and is cold in nature. It is mainly used for carbuncle, breast abscess, scrofula, red eyes, sore throat, lung abscess, intestinal abscess, damp-heat jaundice, hot and painful stranguria, etc.

[0003] Dandelion extract is often combined with other natural ingredients and developed into health products with unique functions to meet consumers' health needs. For example, Chinese patent CN114425074A discloses a natural compound oral liquid for antiviral and immunity enhancement and a preparation method, which includes: 3-5 parts of allicin, 10-15 parts of cabbage extract, 10-20 parts of dandelion extract, 3-5 parts of platycodon extract, 10-12 parts of honeysuckle extract, 3-5 parts of cornus officinalis, 8-10 parts of mulberry, 0.5-2.5 parts of burdock extract, 0.5-1 parts of houttuynia cordata extract, 3-5 parts of astragalus polysaccharide, 1-2 parts of turmeric extract, 1-2 parts of ganoderma lucidum powder, 5-10 parts of licorice extract, 5-8 parts of isatis root, 3-5 parts of glucose. The oral liquid is compounded with natural plant extracts, which can significantly enhance human immunity while killing various viral pathogens, and has no toxic side effects. However, the oral liquid is similar to most natural compound health products / medicines, which are a combination of multiple natural ingredients. However, the oral liquid contains many extracts, and the extraction methods of the various extracts are different, so that the preparation process of the oral liquid is extremely cumbersome and complicated. Although the raw materials used are basically not rare and expensive natural substances, the cumbersome process undoubtedly increases its economic cost. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings of the above-mentioned background technology and provide an oral liquid containing dandelion extract. As one of the extracts of dandelion, dandelion peptide has antibacterial, antiseptic, anti-inflammatory, antioxidant, anti-aging, anti-tumor and other biological activities, and is widely used in food, beverages, and health products. The present invention uses a unique process to simultaneously prepare dandelion extracts rich in dandelion peptides, as well as iris extracts, forsythia extracts, Stephania extracts, platycodon extracts, honeysuckle extracts, burdock extracts, houttuynia extracts, turmeric extracts, and licorice extracts. The oral liquid prepared by compounding dandelion extracts with other natural ingredients has antibacterial and antiviral effects and can be used to improve immunity.

[0005] To achieve the purpose of the present invention, the oral liquid containing dandelion extract of the present invention contains 80-150 parts of dandelion, 50-80 parts of iris, 30-50 parts of forsythia, 50-80 parts of stephania, 30-50 parts of platycodon, 100-120 parts of honeysuckle, 10-30 parts of burdock fruit, 10-20 parts of houttuynia, 10-20 parts of turmeric, 50-100 parts of licorice, 5-10 parts of spirulina extract, and 3-5 parts of glucose, wherein dandelion, iris, forsythia, stephania, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric, and licorice are present in the oral liquid containing dandelion extract in the form of dandelion extract, iris, forsythia, stephania, platycodon, honeysuckle extract, burdock fruit extract, houttuynia extract, turmeric extract, and licorice extract, respectively.

[0006] Further, in some embodiments of the present invention, the preparation methods of the dandelion extract, ilex pubescens extract, forsythia suspensa extract, stephania jasminoides extract, platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and licorice extract are the same, all comprising: (1) Adding deionized water in an amount of 3-5 times the weight of the raw materials to dried dandelion, ilex, forsythia, schizonepeta, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric, and licorice raw materials, and then heating to boiling, boiling for 1-2 hours, and then cooling to 55-60° C., adding 0.1-0.2 times the weight of the raw materials of cellulase and 0.05-0.1 times the weight of the raw materials of neutral protease, and performing enzymolysis under stirring at a stirring rate of 100-150 rpm, after 25-45 minutes, heating to 85-90° C., and filtering after 10-15 minutes to obtain a first filtrate and a first filter residue; (2) drying the first filter residue, adding 80-90% ethanol in an amount of 2-3 times its weight to the dried first filter residue, soaking for 1-2 hours, heating to 55° C.-65° C., extracting for 1-2 hours, stirring once every 25-35 minutes during the extraction process, and stirring at a rate of 100-150 rpm; then standing at 6-9° C. for 20-26 hours, and filtering and separating to obtain a second filtrate and a second filter residue; (3) placing the second filter residue in a fermentation tank, adding deionized water in an amount of 2-3 times the weight of the second filter residue, cellulase in an amount of 0.1-0.2 times the weight of the second filter residue, and pectinase in an amount of 0.1-0.3 times the weight of the second filter residue, adjusting the pH to 6-7, and fermenting at 30-40° C. After fermenting for 16-22 hours, filtering and separating the third filtrate; (4) The first filtrate, the second filtrate and the third filtrate are combined to obtain a crude extract; the crude extract is passed through a macroporous adsorption resin at a flow rate of 3-5 BV / h; the crude extract discharged from the macroporous adsorption resin is passed through an anion resin column and a cation resin column in sequence at a flow rate of 2-4 BV / h; the crude extract discharged from the anion resin column and the cation resin column is passed through an activated carbon moving bed at a flow rate of 35-45 mL / min to obtain a refined extract, and the refined extract is concentrated and dried, and then crushed to obtain a finished extract.

[0007] Furthermore, in some embodiments of the present invention, the method for preparing the oral liquid containing dandelion extract comprises the following steps: (1) taking dried dandelion, iris, forsythia, radix nephrodisiac, platycodon, honeysuckle, burdock fruit, houttuynia cordata, turmeric and licorice according to required weight proportions to prepare dandelion extract, iris, forsythia extract, radix nephrodisiac, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and licorice extract; (2) Weigh the spirulina extract and glucose in the required amounts, add them to the prepared dandelion extract, ilex pubescens extract, forsythia suspensa extract, schizonepeta tenuifolia extract, platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and liquorice extract, stir evenly, place the resulting mixture in a vacuum concentration tank, and perform vacuum concentration at 50-60° C. to obtain a concentrated solution with a relative density of 1.0-1.1 at 80° C., add 2-2.5 times the weight of distilled water to the concentrated solution, and stir evenly to obtain the oral solution containing dandelion extract.

[0008] Furthermore, in some embodiments of the present invention, the enzymatic activity of the cellulase is 30-40 U / mg.

[0009] Furthermore, in some embodiments of the present invention, the enzymatic activity of the neutral protease is 30-40 U / mg.

[0010] Furthermore, in some embodiments of the present invention, the pH is adjusted using citric acid.

[0011] Furthermore, in some embodiments of the present invention, the macroporous adsorption resin is a D-101 macroporous resin.

[0012] Compared with the prior art, the advantages of the present invention are as follows: (1) The preparation methods of dandelion extract, ilex tomentosa extract, forsythia extract, schizonepeta tenuifolia extract, platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract, and licorice extract in the oral liquid of the present invention are improved compared with the prior art, and cellulase is added multiple times, supplemented with neutral protease and pectinase, thereby improving the extraction efficiency and activity of the active substances, and the oral liquid finally obtained has stronger antibacterial and antiviral capabilities; (2) Compared with the natural compound oral liquid for anti-virus and enhancing immunity disclosed in CN114425074A described in the background art, the present invention does not need to add allicin, cabbage extract, astragalus polysaccharide, cornus officinalis, mulberry, isatis root, ganoderma lucidum powder, etc., but only changes the extraction method of active substances in dandelion, platycodon, honeysuckle, burdock, houttuynia, turmeric and licorice, and additionally adds spirulina extract and three raw materials of ilex pubescens, forsythia and suspensa. This not only ensures the oral liquid's antibacterial and antiviral effects and immunity-enhancing effects, but also the overall process is relatively simple. There is no need to additionally prepare allicin, cabbage extract and astragalus polysaccharide. It only needs to mix the spirulina extract, glucose and other raw materials to extract the obtained substances for mixed preparation. DETAILED DESCRIPTION

[0013] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is further described in detail below in conjunction with embodiments. Additional aspects and advantages of the present invention will be given in part in the following description, and part will become apparent from the following description, or will be understood through the practice of the present invention. It should be understood that the following description is only used to explain the present invention and is not intended to limit the present invention.

[0014] As used herein, the terms "comprises," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0015] When amount, concentration or other value or parameter is expressed as range, preferred range or a series of upper preferred value and lower preferred value limit range, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1 to 5", described range should be interpreted as including range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0016] Singular forms include plural references unless the context clearly indicates otherwise. "Optional" or "either" means that the subsequently described event or incident may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

[0017] In addition, the descriptions of the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" described below mean that the specific features, structures, materials, or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment or example. Moreover, the technical features involved in the various embodiments of the present invention can be combined with each other as long as they do not conflict with each other.

[0018] The enzymatic activities of the cellulase and neutral protease in the present invention are 35U / mg, the cellulase, neutral protease and spirulina extract are all conventional commercially available products, and the dried dandelion, ilex, forsythia, truncatum, platycodon, honeysuckle, burdock fruit, houttuynia cordata, turmeric and licorice are all commercially available Chinese medicinal materials. Example

[0019] An oral liquid containing dandelion extract comprises 120 parts of dandelion, 70 parts of ilex tomentosa, 40 parts of forsythia, 65 parts of stephania japonica, 40 parts of platycodon, 110 parts of honeysuckle, 20 parts of burdock fruit, 15 parts of houttuynia cordata, 15 parts of turmeric, 75 parts of licorice, 7 parts of spirulina extract and 4 parts of glucose. The dandelion, ilex tomentosa, forsythia, stephania japonica, platycodon, honeysuckle, burdock fruit, houttuynia cordata, turmeric and licorice are respectively present in the oral liquid containing dandelion extract in the form of dandelion extract, ilex tomentosa extract, forsythia extract, stephania japonica extract, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and licorice extract.

[0020] The oral solution containing the dandelion extract is prepared as follows: (1) Adding deionized water in an amount of 4 times the weight of the raw materials to dried dandelion, ilex, forsythia, schizonepeta, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric, and licorice raw materials, and then heating to boiling, boiling for 1.5 hours, and then cooling to 56° C., adding 0.15 times the weight of the raw materials of cellulase and 0.08 times the weight of the raw materials of neutral protease, and performing enzymolysis under stirring at a stirring rate of 130 rpm, after 35 minutes, heating to 88° C., and filtering after 15 minutes to obtain a first filtrate and a first filter residue; (2) drying the first filter residue, adding 85% ethanol in an amount of 2.5 times its weight to the dried first filter residue, soaking for 1.5 hours, heating to 60° C., extracting for 1.5 hours, stirring once every 30 minutes during the extraction process, and the stirring rate is 120 rpm; then standing at 6-9° C. for 24 hours, and filtering and separating to obtain a second filtrate and a second filter residue; (3) placing the second filter residue in a fermentation tank, adding deionized water in an amount of 2.5 times the weight of the second filter residue and cellulase and pectinase in an amount of 0.15 times the weight of the second filter residue, adjusting the pH to 7, and fermenting at 35° C. After fermenting for 20 hours, filtering and separating the third filtrate; (4) combining the first filtrate, the second filtrate and the third filtrate to obtain a crude extract; passing the crude extract through a macroporous adsorption resin at a flow rate of 3-5 BV / h; passing the crude extract discharged from the macroporous adsorption resin through an anion resin column and a cation resin column in sequence at a flow rate of 2-4 BV / h; passing the crude extract discharged from the anion resin column and the cation resin column through an activated carbon moving bed at a flow rate of 35-45 mL / min to obtain a refined extract, and then concentrating and drying the refined extract, and crushing it to obtain a finished extract; (5) Weigh the spirulina extract and glucose in the required amounts, add them to the prepared dandelion extract, ilex pubescens extract, forsythia suspensa extract, truncatula extract, platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and liquorice extract, stir evenly, place the resulting mixture in a vacuum concentration tank, and perform vacuum concentration at 55°C to obtain a concentrated solution with a relative density of 1.0-1.1 at 80°C, add 2.5 times the weight of distilled water to the concentrated solution, stir evenly to obtain the oral solution containing dandelion extract. Example

[0021] An oral liquid containing dandelion extract comprises 80 parts of dandelion, 50 parts of ilex pubescens, 30 parts of forsythia, 50 parts of stephania japonica, 30 parts of platycodon, 100 parts of honeysuckle, 10 parts of burdock fruit, 10 parts of houttuynia cordata, 10 parts of turmeric, 50 parts of licorice, 5 parts of spirulina extract and 3 parts of glucose. The dandelion, ilex pubescens, forsythia, stephania japonica, platycodon, honeysuckle, burdock fruit, houttuynia cordata, turmeric and licorice are respectively present in the oral liquid containing dandelion extract in the form of dandelion extract, ilex pubescens extract, forsythia extract, stephania japonica extract, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and licorice extract.

[0022] The oral solution containing the dandelion extract is prepared as follows: (1) Add deionized water 3 times the weight of the raw materials to dried dandelion, ilex, forsythia, schizonepeta, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric, and licorice, and then heat to boiling. After boiling for 1.5 hours, cool to 60° C., add 0.2 times the weight of the raw materials of cellulase and 0.1 times the weight of the raw materials of neutral protease, and perform enzymolysis under stirring at a stirring rate of 100 rpm. After 35 minutes, heat to 85° C., and filter after 15 minutes to obtain a first filtrate and a first filter residue; (2) drying the first filter residue, adding 85% ethanol in an amount twice its weight to the dried first filter residue, soaking for 2 hours, heating to 55° C., extracting for 2 hours, stirring once every 25 minutes during the extraction process, and stirring at a rate of 120 rpm; then standing at 6-9° C. for 26 hours, and filtering and separating to obtain a second filtrate and a second filter residue; (3) placing the second filter residue in a fermentation tank, adding deionized water twice the weight of the second filter residue, cellulase 0.15 times the weight of the second filter residue, and pectinase 0.2 times the weight of the second filter residue, adjusting the pH to 7, and fermenting at 35° C. After fermenting for 20 hours, filtering and separating the third filtrate; (4) combining the first filtrate, the second filtrate and the third filtrate to obtain a crude extract; passing the crude extract through a macroporous adsorption resin at a flow rate of 3-5 BV / h; passing the crude extract discharged from the macroporous adsorption resin through an anion resin column and a cation resin column in sequence at a flow rate of 2-4 BV / h; passing the crude extract discharged from the anion resin column and the cation resin column through an activated carbon moving bed at a flow rate of 35-45 mL / min to obtain a refined extract, and then concentrating and drying the refined extract, and crushing it to obtain a finished extract; (5) Weigh the spirulina extract and glucose in the required amounts, add them to the prepared dandelion extract, ilex pubescens extract, forsythia suspensa extract, truncatula extract, platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and liquorice extract, stir evenly, place the resulting mixture in a vacuum concentration tank, and perform vacuum concentration at 55° C. to obtain a concentrated solution with a relative density of 1.0-1.1 at 80° C., add distilled water in an amount twice its weight to the concentrated solution, and stir evenly to obtain the oral solution containing dandelion extract. Example

[0023] An oral liquid containing dandelion extract comprises 150 parts of dandelion, 80 parts of iris, 50 parts of forsythia, 80 parts of stephania, 50 parts of platycodon, 120 parts of honeysuckle, 30 parts of burdock fruit, 20 parts of houttuynia, 20 parts of turmeric, 100 parts of licorice, 10 parts of spirulina extract and 5 parts of glucose. The dandelion, iris, forsythia, stephania, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric and licorice are respectively present in the oral liquid containing dandelion extract in the form of dandelion extract, iris, forsythia extract, stephania extract, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia extract, turmeric extract and licorice extract.

[0024] The oral solution containing the dandelion extract is prepared as follows: (1) Add 5 times the weight of deionized water to dried dandelion, ilex, forsythia, schizonepeta, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric, and licorice raw materials, and then heat to boiling, boil for 2 hours, then cool to 56° C., add 0.15 times the weight of cellulase and 0.1 times the weight of neutral protease, and perform enzymolysis under stirring at a stirring rate of 150 rpm. After 40 minutes, heat to 86° C., and filter after 15 minutes to obtain a first filtrate and a first filter residue; (2) drying the first filter residue, adding 85% ethanol in an amount of 3 times its weight to the dried first filter residue, soaking for 2 hours, heating to 60° C., extracting for 2 hours, stirring once every 30 minutes during the extraction process, and stirring at a rate of 120 rpm; then standing at 6-9° C. for 22 hours, and filtering and separating to obtain a second filtrate and a second filter residue; (3) placing the second filter residue in a fermentation tank, adding deionized water in an amount of 2.5 times the weight of the second filter residue, cellulase in an amount of 0.2 times the weight of the second filter residue, and pectinase in an amount of 0.3 times the weight of the second filter residue, adjusting the pH to 7, and fermenting at 35° C. After fermenting for 20 hours, filtering and separating the third filtrate; (4) combining the first filtrate, the second filtrate and the third filtrate to obtain a crude extract; passing the crude extract through a macroporous adsorption resin at a flow rate of 3-5 BV / h; passing the crude extract discharged from the macroporous adsorption resin through an anion resin column and a cation resin column in sequence at a flow rate of 2-4 BV / h; passing the crude extract discharged from the anion resin column and the cation resin column through an activated carbon moving bed at a flow rate of 35-45 mL / min to obtain a refined extract, and then concentrating and drying the refined extract, and crushing it to obtain a finished extract; (5) Weigh the spirulina extract and glucose in the required amounts, add them to the prepared dandelion extract, ilex pubescens extract, forsythia suspensa extract, truncatula extract, platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and liquorice extract, stir evenly, place the resulting mixture in a vacuum concentration tank, and perform vacuum concentration at 55°C to obtain a concentrated solution with a relative density of 1.0-1.1 at 80°C, add 2.5 times the weight of distilled water to the concentrated solution, stir evenly to obtain the oral solution containing dandelion extract. Example

[0025] An oral liquid containing dandelion extract comprises 120 parts of dandelion, 40 parts of forsythia, 65 parts of stephania japonica, 40 parts of platycodon, 110 parts of honeysuckle, 20 parts of burdock fruit, 15 parts of houttuynia, 15 parts of turmeric, 75 parts of licorice, 7 parts of spirulina extract and 4 parts of glucose. The dandelion, forsythia, stephania japonica, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric and licorice are respectively present in the oral liquid containing dandelion extract in the form of dandelion extract, forsythia extract, stephania japonica extract, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia extract, turmeric extract and licorice extract.

[0026] The oral solution containing the dandelion extract is prepared as follows: (1) Adding deionized water in an amount of 4 times the weight of the raw materials to dried dandelion, forsythia, Stephania, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric, and licorice raw materials, and then heating to boiling, boiling for 1.5 hours, cooling to 56° C., adding 0.15 times the weight of the raw materials of cellulase and 0.08 times the weight of the raw materials of neutral protease, and performing enzymolysis under stirring at a stirring rate of 130 rpm. After 35 minutes, heating to 88° C., and filtering after 15 minutes to obtain a first filtrate and a first filter residue; (2) drying the first filter residue, adding 85% ethanol in an amount of 2.5 times its weight to the dried first filter residue, soaking for 1.5 hours, heating to 60° C., extracting for 1.5 hours, stirring once every 30 minutes during the extraction process, and the stirring rate is 120 rpm; then standing at 6-9° C. for 24 hours, and filtering and separating to obtain a second filtrate and a second filter residue; (3) placing the second filter residue in a fermentation tank, adding deionized water in an amount of 2.5 times the weight of the second filter residue and cellulase and pectinase in an amount of 0.15 times the weight of the second filter residue, adjusting the pH to 7, and fermenting at 35° C. After fermenting for 20 hours, filtering and separating the third filtrate; (4) combining the first filtrate, the second filtrate and the third filtrate to obtain a crude extract; passing the crude extract through a macroporous adsorption resin at a flow rate of 3-5 BV / h; passing the crude extract discharged from the macroporous adsorption resin through an anion resin column and a cation resin column in sequence at a flow rate of 2-4 BV / h; passing the crude extract discharged from the anion resin column and the cation resin column through an activated carbon moving bed at a flow rate of 35-45 mL / min to obtain a refined extract, and then concentrating and drying the refined extract, and crushing it to obtain a finished extract; (5) Weigh the spirulina extract and glucose in the required amounts, add them to the prepared dandelion extract, forsythia extract, Stephania japonica extract, Platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and liquorice extract, stir evenly, place the resulting mixture in a vacuum concentration tank, and perform vacuum concentration at 55°C to obtain a concentrated solution with a relative density of 1.0-1.1 at 80°C, add 2.5 times the weight of distilled water to the concentrated solution, stir evenly to obtain the oral solution containing dandelion extract. Example

[0027] An oral liquid containing dandelion extract comprises 120 parts of dandelion, 70 parts of iris, 65 parts of stephania, 40 parts of platycodon, 110 parts of honeysuckle, 20 parts of burdock fruit, 15 parts of houttuynia, 15 parts of turmeric, 75 parts of licorice, 7 parts of spirulina extract and 4 parts of glucose. The dandelion, iris, stephania, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric and licorice are respectively present in the oral liquid containing dandelion extract in the form of dandelion extract, iris, stephania, platycodon, honeysuckle extract, burdock fruit extract, houttuynia extract, turmeric extract and licorice extract.

[0028] The oral solution containing the dandelion extract is prepared as follows: (1) Adding deionized water in an amount of 4 times the weight of the raw materials to dried dandelion, ilex, Stephania, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric, and licorice raw materials, and then heating to boiling, boiling for 1.5 hours, cooling to 56° C., adding 0.15 times the weight of the raw materials of cellulase and 0.08 times the weight of the raw materials of neutral protease, and performing enzymolysis under stirring at a stirring rate of 130 rpm. After 35 minutes, heating to 88° C., and filtering after 15 minutes to obtain a first filtrate and a first filter residue; (2) drying the first filter residue, adding 85% ethanol in an amount of 2.5 times its weight to the dried first filter residue, soaking for 1.5 hours, heating to 60° C., extracting for 1.5 hours, stirring once every 30 minutes during the extraction process, and the stirring rate is 120 rpm; then standing at 6-9° C. for 24 hours, and filtering and separating to obtain a second filtrate and a second filter residue; (3) placing the second filter residue in a fermentation tank, adding deionized water in an amount of 2.5 times the weight of the second filter residue and cellulase and pectinase in an amount of 0.15 times the weight of the second filter residue, adjusting the pH to 7, and fermenting at 35° C. After fermenting for 20 hours, filtering and separating the third filtrate; (4) combining the first filtrate, the second filtrate and the third filtrate to obtain a crude extract; passing the crude extract through a macroporous adsorption resin at a flow rate of 3-5 BV / h; passing the crude extract discharged from the macroporous adsorption resin through an anion resin column and a cation resin column in sequence at a flow rate of 2-4 BV / h; passing the crude extract discharged from the anion resin column and the cation resin column through an activated carbon moving bed at a flow rate of 35-45 mL / min to obtain a refined extract, and then concentrating and drying the refined extract, and crushing it to obtain a finished extract; (5) Weigh the spirulina extract and glucose in the required amounts, add them to the prepared dandelion extract, ilex pubescens extract, Stephania tetrandra extract, Platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and liquorice extract, stir evenly, place the resulting mixture in a vacuum concentration tank, and perform vacuum concentration at 55° C. to obtain a concentrated solution with a relative density of 1.0-1.1 at 80° C., add 2.5 times the weight of distilled water to the concentrated solution, stir evenly to obtain the oral solution containing dandelion extract. Example

[0029] An oral liquid containing dandelion extract comprises 120 parts of dandelion, 70 parts of ilex pubescens, 40 parts of forsythia, 40 parts of platycodon, 110 parts of honeysuckle, 20 parts of burdock fruit, 15 parts of houttuynia cordata, 15 parts of turmeric, 75 parts of licorice, 7 parts of spirulina extract and 4 parts of glucose. The dandelion, ilex pubescens, forsythia, platycodon, honeysuckle, burdock fruit, houttuynia cordata, turmeric and licorice are respectively present in the oral liquid containing dandelion extract in the form of dandelion extract, ilex pubescens extract, forsythia extract, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and licorice extract.

[0030] The oral solution containing the dandelion extract is prepared as follows: (1) Adding deionized water in an amount of 4 times the weight of the raw materials to dried dandelion, ilex, forsythia, platycodon, honeysuckle, burdock fruit, houttuynia cordata, turmeric, and licorice raw materials, and then heating to boiling, boiling for 1.5 hours, and then cooling to 56° C., adding 0.15 times the weight of the raw materials of cellulase and 0.08 times the weight of the raw materials of neutral protease, and performing enzymolysis under stirring at a stirring rate of 130 rpm. After 35 minutes, the temperature is raised to 88° C., and filtered after 15 minutes to obtain a first filtrate and a first filter residue; (2) drying the first filter residue, adding 85% ethanol in an amount of 2.5 times its weight to the dried first filter residue, soaking for 1.5 hours, heating to 60° C., extracting for 1.5 hours, stirring once every 30 minutes during the extraction process, and the stirring rate is 120 rpm; then standing at 6-9° C. for 24 hours, and filtering and separating to obtain a second filtrate and a second filter residue; (3) placing the second filter residue in a fermentation tank, adding deionized water in an amount of 2.5 times the weight of the second filter residue and cellulase and pectinase in an amount of 0.15 times the weight of the second filter residue, adjusting the pH to 7, and fermenting at 35° C. After fermenting for 20 hours, filtering and separating the third filtrate; (4) combining the first filtrate, the second filtrate and the third filtrate to obtain a crude extract; passing the crude extract through a macroporous adsorption resin at a flow rate of 3-5 BV / h; passing the crude extract discharged from the macroporous adsorption resin through an anion resin column and a cation resin column in sequence at a flow rate of 2-4 BV / h; passing the crude extract discharged from the anion resin column and the cation resin column through an activated carbon moving bed at a flow rate of 35-45 mL / min to obtain a refined extract, and then concentrating and drying the refined extract, and crushing it to obtain a finished extract; (5) Weigh the spirulina extract and glucose in the required amounts, add them to the prepared dandelion extract, ilex pubescens extract, forsythia extract, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and liquorice extract, stir evenly, place the resulting mixture in a vacuum concentration tank, and perform vacuum concentration at 55°C to obtain a concentrated solution with a relative density of 1.0-1.1 at 80°C, add 2.5 times the weight of distilled water to the concentrated solution, stir evenly to obtain the oral solution containing dandelion extract. Example

[0031] An oral liquid containing dandelion extract comprises 120 parts of dandelion, 70 parts of ilex pubescens, 40 parts of forsythia, 65 parts of stephania japonica, 40 parts of platycodon, 110 parts of honeysuckle, 20 parts of burdock fruit, 15 parts of houttuynia cordata, 15 parts of turmeric, 75 parts of licorice and 4 parts of glucose. The dandelion, ilex pubescens, forsythia, stephania japonica, platycodon, honeysuckle, burdock fruit, houttuynia cordata, turmeric and licorice are respectively present in the oral liquid containing dandelion extract in the form of dandelion extract, ilex pubescens extract, forsythia extract, stephania japonica extract, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and licorice extract.

[0032] The oral solution containing the dandelion extract is prepared as follows: (1) Adding deionized water in an amount of 4 times the weight of the raw materials to dried dandelion, ilex, forsythia, schizonepeta, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric, and licorice raw materials, and then heating to boiling, boiling for 1.5 hours, and then cooling to 56° C., adding 0.15 times the weight of the raw materials of cellulase and 0.08 times the weight of the raw materials of neutral protease, and performing enzymolysis under stirring at a stirring rate of 130 rpm, after 35 minutes, heating to 88° C., and filtering after 15 minutes to obtain a first filtrate and a first filter residue; (2) drying the first filter residue, adding 85% ethanol in an amount of 2.5 times its weight to the dried first filter residue, soaking for 1.5 hours, heating to 60° C., extracting for 1.5 hours, stirring once every 30 minutes during the extraction process, and the stirring rate is 120 rpm; then standing at 6-9° C. for 24 hours, and filtering and separating to obtain a second filtrate and a second filter residue; (3) placing the second filter residue in a fermentation tank, adding deionized water in an amount of 2.5 times the weight of the second filter residue and cellulase and pectinase in an amount of 0.15 times the weight of the second filter residue, adjusting the pH to 7, and fermenting at 35° C. After fermenting for 20 hours, filtering and separating the third filtrate; (4) combining the first filtrate, the second filtrate and the third filtrate to obtain a crude extract; passing the crude extract through a macroporous adsorption resin at a flow rate of 3-5 BV / h; passing the crude extract discharged from the macroporous adsorption resin through an anion resin column and a cation resin column in sequence at a flow rate of 2-4 BV / h; passing the crude extract discharged from the anion resin column and the cation resin column through an activated carbon moving bed at a flow rate of 35-45 mL / min to obtain a refined extract, and then concentrating and drying the refined extract, and crushing it to obtain a finished extract; (5) Weigh glucose in the required amount, add it to the prepared dandelion extract, ilex pubescens extract, forsythia suspensa extract, schizonepeta tenuifolia extract, platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and liquorice extract, stir evenly, place the resulting mixture in a vacuum concentration tank, and perform vacuum concentration at 55° C. to obtain a concentrated solution having a relative density of 1.0-1.1 at 80° C., add 2.5 times the weight of distilled water to the concentrated solution, stir evenly, and obtain the oral solution containing dandelion extract. Example

[0033] (1) Mouse mortality and lung index detection According to the method of Chinese patent CN114425074A, 200 SPF mice were selected and randomly divided into: a normal control group, a virus control group, a positive control group and an oral liquid group (administered with the oral liquid containing dandelion extract in Examples 1-7 of the present invention respectively), and the mice were infected with diluted influenza virus drops in the nose under light ether anesthesia, with 20 SPF mice in each group.

[0034] The drug was administered by gavage 2 hours after the virus attack, wherein the normal control group and the virus control group were only supplied with the same volume of physiological saline; in the positive control group, ribavirin was first diluted with distilled water and administered at 70 mg / kg; the oral liquid group was gavaged with the oral liquid containing dandelion extract of the present invention at a dosage of 1.5 g / kg. Once a day, for a total of 1 week, during which the weight change and mortality of the mice before and after the administration were recorded, and the lung index was calculated. The test results are shown in Table 1.

[0035]

[0036] It can be seen from the above Table 1 that the lung index and mortality rate of mice in the oral solution group are significantly reduced compared with the virus control group, indicating that the oral solution prepared by the oral solution in the present invention combines dandelion extract with other natural ingredients with a variety of natural antiviral components and has antiviral effects. It can be used to control the reproduction of viruses in mice and improve the immunity of mice.

[0037] 50,000 MDCK cells were cultured in a 24-well plate and 5 TCID 50 / ml added with influenza A H1N1 / rhinovirus 1A (i.e., HRV-1A); at 33°C, 5% CO 2 Incubate in the incubator for 1 h, wash off the virus solution with Hanks solution, add cell maintenance solution (100 μL of the oral solution of Example 1-7 diluted 4 times, positive control group: 100 μL of 40 μg / mL ribavirin), set up virus control group and cell control group at 33°C, 5% CO 2The observation was continued for 5 days in the incubator, and the results are shown in Table 2. “-” means no cytopathic effect, “****” means more than 3 / 4 cytopathic effect, “***” means 1 / 2-3 / 4 cytopathic effect, “**” means 1 / 4-1 / 2 cytopathic effect, “*” means 1 / 2-3 / 4 cytopathic effect, and “--” means delayed cytopathic effect.

[0038]

[0039] It can be seen from Table 2 that after treatment with the oral liquid of the present invention, the number of cytopathic effects was significantly reduced or delayed, indicating that the reproduction of influenza A (H1N1) virus and HRV-1A virus in mice was effectively inhibited.

[0040] Paratyphoid A and Enterobacterium were selected as the strains to be detected and divided into a control group and an oral liquid group of Examples 1-7. The control group used a conventional culture medium corresponding to the strain, and the experimental group used a conventional culture medium corresponding to the strain and added with 10% by mass fraction of the oral liquid of the present invention. The control group and the oral liquid group were inoculated with equal amounts of the corresponding strains, and the colony results were observed after culturing for 24 h and 48 h. The results are shown in Table 3, where "+" represents the relative number of colonies, the more "+"s there are, the more relatively the number of colonies there are, and "-" represents no colonies.

[0041]

[0042] It can be seen from Table 3 that: (1) during the culture period, at 24 h and 48 h, the number of colonies in the oral liquid group was significantly less than that in the control group; (2) in general, compared with the control group, the colony growth rate of the experimental group was lower than that of the oral liquid group, indicating that the oral liquid of the present invention is rich in antibacterial ingredients and can effectively inhibit bacteria, thereby improving the body's immunity.

[0043] It will be easily understood by those skilled in the art that the above description is merely an example of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An oral solution containing dandelion extract, characterized in that: The oral liquid comprises the following raw materials: 80-150 parts of dandelion, 50-80 parts of ilex pubescens, 30-50 parts of forsythia, 50-80 parts of stephania japonica, 30-50 parts of platycodon, 100-120 parts of honeysuckle, 10-30 parts of burdock fruit, 10-20 parts of houttuynia cordata, 10-20 parts of turmeric, 50-100 parts of licorice, 5-10 parts of spirulina extract, and 3-5 parts of glucose; wherein dandelion, ilex pubescens, forsythia, stephania japonica, platycodon, honeysuckle, burdock fruit, houttuynia cordata, turmeric, and licorice are respectively present in the oral liquid comprising dandelion extract in the form of dandelion extract, ilex pubescens extract, forsythia extract, stephania japonica extract, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract, and licorice extract.

2. The oral liquid containing dandelion extract according to claim 1, characterized in that The preparation methods of the dandelion extract, ilex tomentosa extract, forsythia suspensa extract, stephanotis extract, platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and licorice extract are the same, and all include: (1) Adding deionized water in an amount of 3-5 times the weight of the raw materials to dried dandelion, ilex, forsythia, schizonepeta, platycodon, honeysuckle, burdock fruit, houttuynia, turmeric, and licorice raw materials, and then heating to boiling, boiling for 1-2 hours, and then cooling to 55-60° C., adding 0.1-0.2 times the weight of the raw materials of cellulase and 0.05-0.1 times the weight of the raw materials of neutral protease, and performing enzymolysis under stirring at a stirring rate of 100-150 rpm, after 25-45 minutes, heating to 85-90° C., and filtering after 10-15 minutes to obtain a first filtrate and a first filter residue; (2) drying the first filter residue, adding 80-90% ethanol in an amount of 2-3 times its weight to the dried first filter residue, soaking for 1-2 hours, heating to 55° C.-65° C., extracting for 1-2 hours, stirring once every 25-35 minutes during the extraction process, and stirring at a rate of 100-150 rpm; then standing at 6-9° C. for 20-26 hours, and filtering and separating to obtain a second filtrate and a second filter residue; (3) placing the second filter residue in a fermentation tank, adding deionized water in an amount of 2-3 times the weight of the second filter residue, cellulase in an amount of 0.1-0.2 times the weight of the second filter residue, and pectinase in an amount of 0.1-0.3 times the weight of the second filter residue, adjusting the pH to 6-7, and fermenting at 30-40° C. After fermenting for 16-22 hours, filtering and separating the third filtrate; (4) The first filtrate, the second filtrate and the third filtrate are combined to obtain a crude extract; the crude extract is passed through a macroporous adsorption resin at a flow rate of 3-5 BV / h; the crude extract discharged from the macroporous adsorption resin is passed through an anion resin column and a cation resin column in sequence at a flow rate of 2-4 BV / h; the crude extract discharged from the anion resin column and the cation resin column is passed through an activated carbon moving bed at a flow rate of 35-45 mL / min to obtain a refined extract, and the refined extract is concentrated and dried, and then crushed to obtain a finished extract.

3. The oral liquid containing dandelion extract according to claim 1, characterized in that The preparation method of the oral liquid comprises the following steps: (1) taking dried dandelion, iris, forsythia, radix nephrodisiac, platycodon, honeysuckle, burdock fruit, houttuynia cordata, turmeric and licorice according to required weight proportions to prepare dandelion extract, iris, forsythia extract, radix nephrodisiac, platycodon extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and licorice extract; (2) Weigh the spirulina extract and glucose in the required amounts, add them to the prepared dandelion extract, ilex pubescens extract, forsythia suspensa extract, schizonepeta tenuifolia extract, platycodon grandiflorum extract, honeysuckle extract, burdock fruit extract, houttuynia cordata extract, turmeric extract and liquorice extract, stir evenly, place the resulting mixture in a vacuum concentration tank, and perform vacuum concentration at 50-60° C. to obtain a concentrated solution with a relative density of 1.0-1.1 at 80° C., add 2-2.5 times the weight of distilled water to the concentrated solution, and stir evenly to obtain the oral solution containing dandelion extract.

4. The oral liquid containing dandelion extract according to claim 1, characterized in that The enzymatic activity of the cellulase is 30-40 U / mg.

5. The oral liquid containing dandelion extract according to claim 1, characterized in that The enzymatic activity of the neutral protease is 30-40 U / mg.

6. The oral liquid containing dandelion extract according to claim 1, characterized in that: The pH is adjusted using citric acid.

7. The oral liquid containing dandelion extract according to claim 1, characterized in that: The macroporous adsorption resin is D-101 macroporous resin.

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