Extraction and separation method of hericium erinaceus polysaccharide

Abstract

The invention discloses an extraction and separation method of hericium erinaceus polysaccharide, and relates to the technical field of biological extraction, the method comprises the following components: S1, molecularly imprinted polymer preparation, S2, hericium erinaceus raw material pretreatment and crude extract preparation, S3, polysaccharide adsorption, S4, polymer separation and eluent collection, and S5, eluent post-treatment and product acquisition. According to the method, efficient adsorption and separation of the hericium erinaceus polysaccharide are achieved by preparing the molecularly imprinted polymer with specific recognition sites on the hericium erinaceus polysaccharide, the hericium erinaceus polysaccharide can be accurately adsorbed from other impurities in a complex extraction system, the purity of the product is improved, and meanwhile, the molecular imprinting polymer with the specific recognition sites on the hericium erinaceus polysaccharide is obtained. The hericium erinaceus polysaccharide can be further purified through subsequent elution, concentration, desalination and drying treatment, and a high-purity product is obtained.

Description

Technical Field

[0001] The invention relates to the technical field of biological extraction, and in particular to a method for extracting and separating Hericium erinaceus polysaccharide. Background Art

[0002] As a precious edible and medicinal fungus, Hericium erinaceus polysaccharides contained in it have multiple biological activities, such as immune regulation, anti-tumor, and hypoglycemic effects. Therefore, it has attracted widespread attention from scientific researchers and the market. In order to make full use of this precious natural resource, researchers are constantly exploring and optimizing the extraction and separation methods of Hericium erinaceus polysaccharides.

[0003] Traditionally, the extraction and separation of Hericium erinaceus polysaccharides mostly use chemical or physical methods, such as acid-base extraction, hot water extraction, and ultrasonic-assisted extraction. However, these methods often have many disadvantages in the extraction process. For example, acid-base extraction may cause damage to the polysaccharide structure and affect its biological activity; although hot water extraction is easy to operate, the extraction efficiency is low and requires a lot of time and energy; although ultrasonic-assisted extraction can improve the extraction efficiency, the equipment cost is high and may introduce the potential impact of ultrasound on the polysaccharide structure. In addition, when dealing with complex systems, traditional methods often find it difficult to effectively separate Hericium erinaceus polysaccharides from other impurities, resulting in low product purity, which limits its further application and development.

[0004] In summary, the traditional extraction and separation methods of Hericium erinaceus polysaccharides have many shortcomings and cannot meet the current market demand for high-quality and high-purity Hericium erinaceus polysaccharides. Therefore, it is particularly important to develop a method for extraction and separation of Hericium erinaceus polysaccharides. Summary of the invention

[0005] The purpose of the present invention is to make up for the shortcomings of the prior art and provide a method for extracting and separating Hericium erinaceus polysaccharides. The method can achieve efficient adsorption and separation of Hericium erinaceus polysaccharides by introducing molecular imprinting polymer technology and utilizing its specific recognition ability for Hericium erinaceus polysaccharides. The method not only improves the purity of the product, but also significantly improves production efficiency and reduces production costs by optimizing process parameters, thereby providing strong technical support for the industrial production of Hericium erinaceus polysaccharides.

[0006] In order to solve the above technical problems, the present invention provides the following technical solution: a method for extracting and separating Hericium erinaceus polysaccharides, the specific steps of the method are:

[0007] S1. Preparation of molecular imprinted polymer: preparing a molecular imprinted polymer having a specific recognition site for Hericium erinaceus polysaccharide, wherein the molecular imprinted polymer is obtained by dissolving a template molecule (Hericium erinaceus polysaccharide), a functional monomer, a cross-linking agent, and an initiator in a porogen, and eluting the template molecule after a polymerization reaction;

[0008] S2. Pretreatment of Hericium erinaceus raw materials and preparation of crude extract: pretreatment of Hericium erinaceus raw materials, and then preparation of Hericium erinaceus polysaccharide crude extract by extraction method;

[0009] S3, polysaccharide adsorption: adding molecular imprinting polymer to the crude extract, allowing the polymer to fully contact with the crude extract, so that the polymer specifically adsorbs Hericium erinaceus polysaccharide;

[0010] S4, polymer separation and eluate collection: separating the molecularly imprinted polymer adsorbed with polysaccharide from the crude extract, then eluting the polymer with an eluent, and collecting the eluate;

[0011] S5. Post-processing of the eluate and acquisition of the product: The collected eluate is concentrated, desalted and dried to finally obtain a high-purity Hericium erinaceus polysaccharide product.

[0012] Furthermore, the functional monomer is selected from one or more of methacrylic acid and acrylamide, and the molar ratio of the functional monomer to the template molecule is (4-8):1.

[0013] Furthermore, the crosslinking agent is ethylene glycol dimethacrylate, and the molar ratio of the crosslinking agent to the functional monomer is (3-6):1.

[0014] Furthermore, the initiator is azobisisobutyronitrile, and the initiator accounts for 0.5%-2% of the total mass of the mixed solution.

[0015] Furthermore, the porogen is toluene or acetonitrile, and the volume proportion of the porogen in the mixed solution is 60%-80%.

[0016] Furthermore, in the process of eluting the template molecule, the amount of eluent used is 10-30 mL of eluent per gram of polymer, and the elution time is 8-16 hours.

[0017] Furthermore, when the Hericium erinaceus raw material is pretreated, the particle size after crushing is 40-80 mesh, and it is dried to a moisture content of less than 8%.

[0018] Furthermore, when the molecularly imprinted polymer is added to the crude extract, the mass of the polymer added to every 100 mL of the crude extract is 2-5 g.

[0019] Furthermore, when eluting the polymer, the elution flow rate of the eluent is controlled at 0.5-2 mL / min.

[0020] Furthermore, the concentrated eluent is prepared by a rotary evaporator, the evaporation temperature is controlled at 40-60°C, and the vacuum degree is 0.06-0.09MPa.

[0021] Compared with the prior art, the method for extracting and separating Hericium erinaceus polysaccharides has the following beneficial effects:

[0022] 1. This method achieves efficient adsorption and separation of Hericium erinaceus polysaccharide by preparing molecular imprinting polymers with specific recognition sites for Hericium erinaceus polysaccharide. This method can accurately adsorb Hericium erinaceus polysaccharide from other impurities in a complex extraction system, thereby improving the purity of the product. At the same time, through subsequent elution, concentration, desalting and drying treatments, Hericium erinaceus polysaccharide can be further purified to obtain a high-purity product.

[0023] 2. This method adopts scientific process design and precise operation steps, including the preparation of molecular imprinting polymer, pretreatment of Hericium erinaceus raw materials, adsorption of polysaccharides, separation and elution of polymers, and post-treatment of eluents. These steps are closely linked to form a complete extraction and separation process. By optimizing the process parameters of each step, such as the molar ratio of functional monomer to template molecule, the molar ratio of cross-linking agent to functional monomer, the amount of eluent and the elution time, the production efficiency can be significantly improved, the production cycle can be shortened, and the production cost can be reduced. At the same time, this method also has good stability and repeatability, and is suitable for large-scale industrial production.

[0024] Other advantages, objectives and features of the present invention will be set forth in part in the following description and, in part, will be apparent to those skilled in the art based on an examination of the following or may be taught from the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0026] Figure 1 This is a process diagram of a method for extracting and separating Hericium erinaceus polysaccharides;

[0027] Figure 2 The present invention is a detailed flow chart of a method for extracting and separating Hericium erinaceus polysaccharides. DETAILED DESCRIPTION

[0028] In order to further explain the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation mode, structure, characteristics and effects of the present invention are described in detail below in combination with the accompanying drawings and preferred embodiments.

[0029] Embodiment 1

[0030] This embodiment describes that in a biochemistry laboratory of a certain university, researchers are committed to studying the biological activity of Hericium erinaceus polysaccharides and need to extract high-purity Hericium erinaceus polysaccharides in small batches as experimental samples. Since the experiment has extremely high requirements for the purity of polysaccharides and the sample size requirement is relatively small, this sophisticated extraction and separation method is adopted.

[0031] Accurately weigh a certain amount of template molecules, and then select methacrylic acid as a functional monomer, and weigh it according to the molar ratio of functional monomer to template molecule of 6:1. Then, add ethylene glycol dimethacrylate as a cross-linking agent, and the molar ratio of cross-linking agent to functional monomer is set to 4:1. These substances are dissolved in toluene porogen together with azobisisobutyronitrile initiator accounting for 1% of the total mass of the mixed solution, and the volume proportion of toluene in the mixed solution is 70%. Use a magnetic stirrer to fully stir the mixed solution to make it uniformly dispersed. Subsequently, transfer the mixed solution to a polymerization reaction container, and carry out polymerization reaction under suitable temperature and reaction conditions. After the reaction, a polymer containing template molecules is obtained. In order to remove the template molecules, the polymer is placed in an elution device, and an eluent is added at a ratio of 20 mL of eluent per gram of polymer. The elution time is set to 12 hours. During the elution process, the elution device is continuously shaken to ensure that the template molecules are fully eluted, and finally a molecular imprinting polymer with a specific recognition site for Hericium erinaceus polysaccharide is obtained.

[0032] Select fresh, pest-free Hericium erinaceus raw materials, rinse them with clean water to remove surface impurities, cut the washed Hericium erinaceus into small pieces, put them into a grinder and grind them to a particle size of 60 mesh, then put the crushed Hericium erinaceus powder into an oven, set the oven temperature to 50°C, and dry it to a water content of less than 8%. The Hericium erinaceus polysaccharide crude extract was prepared by hot water extraction. The pretreated Hericium erinaceus raw materials and deionized water were added to a round-bottom flask at a mass ratio of 1:10, and stirred and extracted in a constant temperature water bath at 80°C for 3 hours. After the extraction, filter it with four layers of gauze while hot to remove the residue to obtain the Hericium erinaceus polysaccharide crude extract.

[0033] Accurately measure 100 mL of the crude extract into a 250 mL conical flask, add 3 g of the prepared molecular imprinting polymer, place the conical flask on a shaker, set the shaker temperature to 30 ° C, the oscillation speed to 150 r / min, and the oscillation time to 4 hours to allow the polymer to fully contact with the crude extract and ensure that the polymer can specifically adsorb Hericium erinaceus polysaccharides. During the oscillation process, it was observed that the solution gradually became turbid, which was the result of the interaction between the polymer and the polysaccharide.

[0034] After the shaking is completed, the molecular imprinting polymer adsorbed with polysaccharides is separated from the crude extract by filtering using a Buchner funnel and a suction filtration device, and the separated polymer is carefully transferred to a chromatography column and eluted with an eluent. The eluent is a methanol aqueous solution with a volume ratio of 80%, and the elution flow rate is controlled at 1 mL / min. During the elution process, the eluate is collected using a collection bottle. As the elution proceeds, it is observed that the eluate gradually becomes clear and the color changes slightly, which indicates that the polysaccharide is gradually eluted.

[0035] The collected eluate was transferred to the distillation bottle of the rotary evaporator, and the evaporation temperature was set to 50°C and the vacuum degree was set to 0.08MPa for concentration. During the concentration process, the volume change of the solution in the distillation bottle was closely observed. When the volume of the solution was concentrated to about 1 / 4 of the original volume, the concentration was stopped. The concentrated solution was desalted by dialysis, and the solution was placed in a dialysis bag and dialyzed in deionized water for 24 hours. The deionized water was replaced 3-4 times during the period. After the dialysis, the solution was freeze-dried. The solution was first pre-frozen to -80°C, and then placed in a freeze dryer and dried under vacuum conditions for 24 hours. Finally, a high-purity Hericium erinaceus polysaccharide product was obtained. The obtained product was tested for purity. The purity was determined by high-performance liquid chromatography to be more than 95%, which met the requirements of scientific research experiments. These high-purity Hericium erinaceus polysaccharide products were used in subsequent cell experiments and animal experiments to study their biological activities in immunomodulation, anti-tumor, etc.

[0036] Embodiment 2

[0037] This embodiment describes the need for large-scale extraction of Hericium erinaceus polysaccharides in a modern production workshop of a health care product manufacturer in order to meet the market demand for health care products containing Hericium erinaceus polysaccharides. Due to the large scale of production, there are high requirements for extraction efficiency and cost control, while ensuring stable product quality.

[0038] In a large reactor, each raw material is accurately measured according to the production formula, acrylamide is used as a functional monomer, and the template molecule is weighed at a molar ratio of 7:1, and ethylene glycol dimethacrylate is added as a crosslinker, and the molar ratio of the crosslinker to the functional monomer is 5:1. These raw materials are dissolved in acetonitrile porogen together with azobisisobutyronitrile initiator accounting for 1.5% of the total mass of the mixed solution, and the volume proportion of acetonitrile in the mixed solution is 75%. The stirring device of the reactor is turned on and stirred at a speed of 300r / min for 30 minutes to fully mix the raw materials. Then, the temperature and pressure of the reactor are controlled to carry out polymerization under appropriate conditions. After the reaction is completed, a large amount of polymer containing template molecules is obtained, and the polymer is transferred to a large elution device, and an eluent is added at a ratio of 25mL of eluent per gram of polymer. The elution time is set to 14 hours. During the elution process, the circulating system of the elution device is used to continuously circulate the eluent to ensure that the template molecules are fully eluted, and finally a large number of molecular imprinted polymers with specific recognition sites for Hericium erinaceus polysaccharides are prepared.

[0039] A large amount of fresh Hericium erinaceus raw materials are purchased from high-quality Hericium erinaceus planting bases. After the raw materials arrive at the production workshop, they are first screened to remove spoiled and damaged Hericium erinaceus. The screened Hericium erinaceus is cleaned by automatic cleaning equipment, and then sent to a large crusher to be crushed to a particle size of 50 mesh. The crushed Hericium erinaceus powder is transported to a drying tower by a conveyor belt. In the drying tower, the Hericium erinaceus powder is dried to a moisture content of less than 8% by hot air drying. Ultrasonic-assisted extraction is used to prepare a crude extract. A large amount of pretreated Hericium erinaceus raw materials are put into a large extraction tank, and deionized water 8 times the mass of the raw materials is added. The ultrasonic equipment is turned on, the ultrasonic power is set to 500W, and the extraction is carried out at 40°C for 2 hours. After the extraction is completed, it is filtered through a plate and frame filter press to remove the residue to obtain a large amount of Hericium erinaceus polysaccharide crude extract.

[0040] 500L of crude extract was added to a large reactor. 4g of molecular imprinting polymer was added to every 100mL of crude extract. 20kg of the prepared molecular imprinting polymer was added through an automatic metering device. The stirring device of the reactor was turned on and stirred at a speed of 200r / min for 6 hours to fully mix the polymer and the crude extract to ensure that the polymer can specifically adsorb Hericium erinaceus polysaccharides. During the stirring process, the concentration and pH value of the solution were monitored in real time by online monitoring equipment to ensure that the adsorption process was carried out under optimal conditions.

[0041] After the stirring is completed, the molecular imprinting polymer adsorbed with polysaccharides is separated from the crude extract by a centrifuge. The speed of the centrifuge is set to 3000r / min, and the centrifugation time is 20 minutes. The separated polymer is transported to a large chromatography column and eluted with an eluent. The eluent is an ethanol aqueous solution with a volume ratio of 70%, and the elution flow rate is controlled at 1.5mL / min. During the elution process, the eluate is collected in sequence through multiple collection tanks, and each collection tank collects a certain volume of eluate, which is convenient for subsequent quality inspection and analysis of the eluate.

[0042] The collected eluate is transported to the large distillation kettle of the rotary evaporator through a pipeline, and the evaporation temperature is set to 55°C and the vacuum degree is 0.07MPa for concentration. During the concentration process, the volume and concentration of the solution in the distillation kettle are monitored in real time by an automated control system. When the solution concentration reaches a certain index, the concentration is stopped. The concentrated solution is desalted by an ion exchange resin method, and the solution is passed through an ion exchange resin column to remove the salt. The desalted solution is dried by a spray drying tower, and the inlet temperature of the spray drying tower is set to 180°C and the outlet temperature is set to 80°C. Finally, a high-purity Hericium erinaceus polysaccharide powder is obtained. The obtained Hericium erinaceus polysaccharide powder is subjected to quality inspection, and all indicators meet the quality standards for the production of health products. These Hericium erinaceus polysaccharide powders are used to produce various Hericium erinaceus health products, such as oral liquids and capsules, to meet market demand and provide consumers with products with health functions.

[0043] Embodiment 3

[0044] This example describes that in an institution focusing on the development of natural medicines, the scientific research team is committed to developing a new drug with Hericium erinaceus polysaccharide as the key active ingredient, hoping to be used to treat specific digestive system diseases. This research and development project has extremely high requirements for the purity and quality of Hericium erinaceus polysaccharide, and requires precise control of various parameters in the extraction process to ensure the consistency and stability of the product and provide reliable samples for subsequent drug clinical trials.

[0045] First, on a well-ventilated chemical operating table, the researchers accurately weighed a certain amount of Hericium erinaceus polysaccharide template molecules. Then, methacrylic acid and acrylamide were selected as functional monomers in a 1:1 mixture. The functional monomers were accurately weighed based on a molar ratio of 5:1 between the functional monomers and the template molecules. Next, the cross-linking agent ethylene glycol dimethacrylate was added to a molar ratio of 3:1 with the functional monomers. These substances were slowly poured into a reaction vessel containing a toluene porogen together with an azobisisobutyronitrile initiator accounting for 0.8% of the total mass of the mixed solution. The volume proportion of toluene in the mixed solution was 65%. The mixture was manually stirred for a while using a stirring rod. After preliminary mixing, the reaction vessel was fixed on a magnetic stirrer and stirred at 200 rpm for 30 minutes to fully mix the components.

[0046] After mixing evenly, the reaction container was transferred to a constant temperature water bath, and the water bath temperature was set to 60°C for polymerization reaction. During the reaction, the researchers closely observed the reaction phenomena and recorded the reaction time. After 6 hours of reaction, a polymer containing the template molecule was obtained.

[0047] In order to remove the template molecules, the polymer was carefully transferred to a Soxhlet extractor, and the eluent was added at a ratio of 15 mL of eluent (90% ethanol aqueous solution by volume) per gram of polymer. The elution time was set to 10 hours. During the elution process, the Soxhlet extractor continued to reflux to ensure that the template molecules were fully eluted. After the elution was completed, the polymer was taken out and repeatedly rinsed with deionized water until no template molecules were detected in the eluate, and finally a molecular imprinting polymer with specific recognition sites for Hericium erinaceus polysaccharide was obtained.

[0048] The raw materials of Hericium erinaceus are collected from multiple high-quality Hericium erinaceus producing areas, and the raw materials are strictly screened to select Hericium erinaceus with complete shape, normal color, and no diseases and insect pests. The selected Hericium erinaceus are rinsed with clean water, and the surface moisture is naturally dried. The Hericium erinaceus is crushed into a particle size of 70 mesh using a professional grinder. The crushed Hericium erinaceus powder is then spread on a tray and placed in a blast drying oven with the temperature set to 45°C and dried until the moisture content is less than 8%.

[0049] The crude extract was prepared by enzymatic hydrolysis. The pretreated Hericium erinaceus powder was added to a clean reactor. Deionized water was added according to a mass ratio of Hericium erinaceus powder to water of 1:15, and the mixture was stirred evenly. Then, appropriate amounts of cellulase and pectinase were added. The enzyme addition amounts were 0.5% and 0.3% of the mass of Hericium erinaceus powder, respectively. The temperature of the reactor was controlled at 40°C, the pH value was adjusted to 5.0, and the reaction was carried out under mild conditions for 4 hours. After the reaction was completed, the mixture in the reactor was heated to 80°C and maintained for 10 minutes to inactivate the enzyme. Subsequently, the mixture was filtered through a plate and frame filter press to obtain a crude Hericium erinaceus polysaccharide extract.

[0050] Accurately measure 100 mL of the crude extract into a 250 mL glass container, add 2.5 g of the prepared molecular imprinting polymer, seal the glass container, and place it in a constant temperature oscillator. Set the temperature to 25 ° C, the oscillation speed to 120 rpm, and the oscillation time to 5 hours to allow the polymer to fully contact with the crude extract to ensure that the polymer specifically adsorbs Hericium erinaceus polysaccharides. During the oscillation process, take out the container every 1 hour and shake it gently to promote uniform adsorption.

[0051] After the oscillation is completed, the molecular imprinting polymer adsorbed with polysaccharides is separated from the crude extract by filtration using a Buchner funnel and a suction filtration bottle, and the separated polymer is carefully transferred to a small chromatography column and eluted with an eluent (a methanol-water solution with a volume ratio of 85%). The elution flow rate is controlled at 0.8 mL / min. During the elution process, multiple clean collection bottles are used to collect the eluate in turn. A collection bottle is replaced every time 5 mL of eluate is collected, and each collection bottle is numbered and marked.

[0052] The collected eluates were combined and transferred to the distillation flask of the rotary evaporator. The evaporation temperature was set to 45°C and the vacuum degree was set to 0.07 MPa for concentration. During the concentration process, the volume change of the solution in the distillation flask was observed. When the solution volume was concentrated to about 1 / 3 of the original volume, the concentration was stopped.

[0053] The concentrated solution is desalted by ultrafiltration. An ultrafiltration membrane with a molecular weight cutoff of 1000Da is selected to filter the solution through an ultrafiltration device to remove salt and small molecular impurities. During the ultrafiltration process, deionized water is continuously added to the solution to improve the desalination effect.

[0054] The desalted solution is freeze-dried. The solution is first transferred to a vial, and an appropriate amount of solution is placed in each vial. The vial is then placed in a pre-freezing refrigerator, and the pre-freezing temperature is set to -80°C for 3 hours. After the pre-freezing is completed, the vial is quickly transferred to a freeze dryer and dried for 24 hours under a vacuum degree of less than 10Pa to finally obtain a high-purity Hericium erinaceus polysaccharide product.

[0055] The obtained Hericium erinaceus polysaccharide product was subjected to comprehensive quality testing, and its purity was determined by high performance liquid chromatography. The results showed that the purity reached more than 98%. Its structure was identified by infrared spectroscopy and nuclear magnetic resonance technology, confirming that it was the target Hericium erinaceus polysaccharide. At the same time, safety indicators such as heavy metal content and microbial limits in the product were tested, and all met the relevant standards for drug research and development. These high-purity Hericium erinaceus polysaccharide products were used for subsequent drug activity screening, pharmacological research and drug preparation development, laying a solid foundation for the research and development of new drugs.

[0056] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A method for extracting and separating Hericium erinaceus polysaccharides, characterized in that: The specific steps of this method are: S1. Preparation of molecular imprinted polymer: preparing a molecular imprinted polymer having a specific recognition site for Hericium erinaceus polysaccharide, wherein the molecular imprinted polymer is obtained by dissolving a template molecule, a functional monomer, a cross-linking agent, and an initiator in a porogen, and eluting the template molecule after a polymerization reaction; S2. Pretreatment of Hericium erinaceus raw materials and preparation of crude extract: pretreatment of Hericium erinaceus raw materials, and then preparation of Hericium erinaceus polysaccharide crude extract by extraction method; S3, polysaccharide adsorption: adding molecular imprinting polymer to the crude extract, allowing the polymer to fully contact with the crude extract, so that the polymer specifically adsorbs Hericium erinaceus polysaccharide; S4, polymer separation and eluate collection: separating the molecularly imprinted polymer adsorbed with polysaccharide from the crude extract, then eluting the polymer with an eluent, and collecting the eluate; S5. Post-processing of the eluate and acquisition of the product: The collected eluate is concentrated, desalted and dried to finally obtain a high-purity Hericium erinaceus polysaccharide product.

2. The method for extracting and separating Hericium erinaceus polysaccharide according to claim 1, characterized in that: The functional monomer is selected from one or more of methacrylic acid and acrylamide, and the molar ratio of the functional monomer to the template molecule is (4-8):

1.

3. The method for extracting and separating Hericium erinaceus polysaccharide according to claim 1, characterized in that: The crosslinking agent is ethylene glycol dimethacrylate, and the molar ratio of the crosslinking agent to the functional monomer is (3-6):

1.

4. The method for extracting and separating Hericium erinaceus polysaccharide according to claim 1, characterized in that: The initiator is azobisisobutyronitrile, and the initiator accounts for 0.5%-2% of the total mass of the mixed solution.

5. The method for extracting and separating Hericium erinaceus polysaccharide according to claim 1, characterized in that: The porogen is toluene or acetonitrile, and the volume proportion of the porogen in the mixed solution is 60%-80%.

6. The method for extracting and separating Hericium erinaceus polysaccharide according to claim 1, characterized in that: During the elution of the template molecules, the amount of eluent used is 10-30 mL of eluent per gram of polymer, and the elution time is 8-16 hours.

7. The method for extracting and separating Hericium erinaceus polysaccharide according to claim 1, characterized in that: When the Hericium erinaceus raw material is pretreated, the particle size after crushing is 40-80 meshes, and it is dried to a water content of less than 8%.

8. The method for extracting and separating Hericium erinaceus polysaccharide according to claim 1, characterized in that: When the molecularly imprinted polymer is added to the crude extract, the mass of the polymer added to every 100 mL of the crude extract is 2-5 g.

9. The method for extracting and separating Hericium erinaceus polysaccharide according to claim 1, characterized in that: When eluting the polymer, the elution flow rate of the eluent is controlled at 0.5-2 mL / min.

10. The method for extracting and separating Hericium erinaceus polysaccharide according to claim 1, characterized in that: The concentrated eluent is prepared by a rotary evaporator, the evaporation temperature is controlled at 40-60° C., and the vacuum degree is 0.06-0.09 MPa.

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