Rapid extraction equipment for cell collection and use method thereof

Abstract

The invention discloses rapid extraction equipment for cell collection and a use method of the rapid extraction equipment, and belongs to the technical field of cell collection and extraction.The rapid extraction equipment is characterized in that a sample is sucked into a turnover cylinder, a sliding plate is supported through elasticity of a second spring, a sealing seat can be tightly attached to a conical groove, and a liquid inlet pipe is automatically blocked to prevent backflow of the sample; the piston column moves downwards so that samples can enter the guide pipes through the one-way valves, the one-way valves can prevent the samples from flowing back into the turnover cylinder, the multiple one-way valves communicate with the turnover cylinder, in the downward pressing process of the piston column, filling can be conducted on the multiple centrifugal tubes at the same time, and the samples in the turnover cylinder are divided through the multiple guide pipes; therefore, the quantitative sampling and extracting work can be conveniently and rapidly completed by collecting the total amount of the sample at a time and then distributing the sample into the multiple centrifugal tubes, the sample in the turnover cylinder does not flow back, and cross contamination is prevented.

Description

Technical Field

[0001] The present invention relates to the technical field of cell collection and extraction, and in particular to a rapid extraction device for cell collection and a use method thereof. Background Art

[0002] Hematological tumors are malignant clonal proliferative diseases caused by the obstruction of differentiation and development of hematopoietic stem cells, including acute and chronic leukemia, lymphoma and multiple myeloma. Their pathogenesis and clinical characteristics are highly heterogeneous, and their prognosis is also very different. Therefore, their clinical diagnosis and treatment require the integration of a series of clinical data; and the acquisition of clinical data mainly relies on MICM integrated diagnosis; MICM integrated diagnosis refers to the comprehensive diagnosis of hematological tumors using morphology and cell chemistry, immunology, cell chromosome karyotype analysis and molecular biology.

[0003] Among them, cell chromosome karyotype analysis is to reveal whether there are abnormalities in chromosomes by culturing cells, examining under a microscope and analyzing the number, morphology and structure of chromosomes of cells. When collecting and extracting cells from various blood tumor samples, a centrifuge is required to separate the supernatant and the lower cells. The centrifuge tubes containing the samples are placed in the centrifuge one by one and tilted in the centrifuge tank, which helps the sample particles slide along the tube wall of the centrifuge tube to the bottom to form a precipitate. The tilted placement of batch centrifuge tubes is inconvenient and increases the workload. Moreover, in the process of placing blood tumor samples in multiple centrifuge tubes, a small amount of samples need to be collected and extracted multiple times, so that multiple measurements are performed on the samples. The direct suction method is difficult to accurately control the various differences in the sampling pipette, so that the collected samples need to be sucked multiple times to complete the quantitative sampling work, and the samples in the pipette will reflux, which is very easy to cause cross contamination. Summary of the invention

[0004] The object of the present invention is to provide a rapid extraction device for cell collection and a method of using the same, so as to solve the problems raised in the above-mentioned background technology that the tilted placement and handling of batch centrifuge tubes is inconvenient and increases the workload; and in the process of placing blood tumor samples in multiple centrifuge tubes, a small amount of samples need to be collected and extracted multiple times, so as to perform multiple measurements on the samples. The direct suction method makes it difficult to accurately control the various differences in the sampling pipette, so that the collected samples need to be aspirated multiple times to complete the quantitative sampling work, and the sample in the pipette may reflux.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A rapid extraction device for cell collection comprises a centrifuge housing, a turnover drum is installed on the centrifuge housing, a bottom of the turnover drum is fixedly connected to a base assembly, a placement groove is provided at the bottom of the centrifuge housing, the bottom of the base assembly is installed in the placement groove, a suction assembly is installed in the turnover drum, a plurality of mounting assemblies are installed outside the base assembly, a centrifuge tube is provided in the mounting assembly, an end cap is clamped on the top of the centrifuge tube, a liquid guide assembly is connected to the end cap, the top of the liquid guide assembly is connected to the bottom of the turnover drum, a liquid inlet pipe is installed at the center of the bottom of the turnover drum, the bottom end of the liquid inlet pipe is connected to an adapter, the bottom of the adapter is connected to an extension pipe, the inner wall of the liquid inlet pipe is provided with a conical groove, an isolation assembly is installed in the conical groove, a baffle cover is provided outside the isolation assembly, the baffle cover is fixed to the bottom of the inner wall of the turnover drum, and a plurality of discharge holes are provided on the outer wall of the baffle cover.

[0007] As a further solution of the present invention, a bearing seat is installed outside the rotating drum, a support plate is clamped outside the bearing seat, the support plate is clamped on the top of the centrifuge housing, two bolts are threadedly connected on both sides of the support plate, and the support plate is fixed to one side of the centrifuge housing by bolts, and the outer wall of the rotating drum is provided with a scale bar.

[0008] As a further solution of the present invention, the suction assembly includes a piston column, which is located in the revolving cylinder. A groove is provided at the bottom of the piston column corresponding to the position of the baffle cover. A pull rod is provided at the top of the piston column. The pull rod is connected to the bottom of the revolving cylinder through a limiting sleeve, and a handle is fixed to the top of the pull rod.

[0009] As a further solution of the present invention, the base support assembly includes a mounting base, a rectangular groove is provided at the bottom of the mounting base, an operating hole is provided at the top of the inner wall of the rectangular groove, the bottom of the mounting base is connected to the bottom of the inner wall of the placement groove, a support frame is fixed to the top of the mounting base, the support frame is fixed to the bottom of the turnover cylinder, openings are respectively provided outside the support frame at positions corresponding to the mounting components, a hinge seat is installed at the top of the inner wall of the opening, and the top of the mounting component is hinged to the support frame through the hinge seat.

[0010] As a further solution of the present invention, the mounting assembly includes a fixed frame, which is L-shaped and has two partition bars fixed to the inner wall of the fixed frame. The bottom end of the centrifuge tube passes through the two partition bars and is clamped with the bottom of the inner wall of the fixed frame. A column is fixed to the bottom of the inner wall of the fixed frame. The top of the column passes through the two partition bars and is connected to a hinge seat. The top of the fixed frame is hinged with a telescopic rod through a pin shaft. The top of the telescopic rod is hinged with the bottom of the rotating drum through a pin shaft, and the outer sleeve of the telescopic rod is connected with a first spring.

[0011] As a further solution of the present invention, the liquid guiding assembly includes a catheter, a one-way valve is installed at the top of the catheter, the one-way valve is connected to the bottom of the revolving cylinder, the bottom end of the catheter is connected to a stop valve, and the bottom end of the stop valve is plugged into the top of the end cover.

[0012] As a further solution of the present invention, the isolation assembly includes a sealing seat, which is conical in design and clamped in the conical groove. A connecting column is fixed to the top of the sealing seat, and a slide is fixed to the bottom of the block cover at the top of the connecting column, and a sliding column is fixed on the slide, which slides through the block cover and is connected to the outer sleeve of the sliding column. The two ends of the second spring are respectively fixed on the slide and the top of the inner wall of the block cover.

[0013] A method for using a rapid extraction device for cell collection, the method comprising the following steps:

[0014] When collecting and extracting cells from a blood tumor sample, the bolts on both sides of the support plate are loosened to release the locking state between the support plate and the centrifuge housing, the support plate is lifted to remove the revolving cylinder and the bottom support assembly from the centrifuge housing, the extension tube is connected to the bottom end of the liquid inlet tube through the adapter, and then the bottom end of the extension tube is extended into the blood tumor sample, the handle is pulled upward, and the handle drives the piston column to move upward through the pull rod. Since the bottom of the revolving cylinder and the piston column are in a sealed state, the volume of the gas in the revolving cylinder changes, thereby causing a change in the gas pressure, causing the slide column to be subjected to an upward thrust, and the slide column drives the slide plate to move upward inside the baffle cover, and the slide plate drives the sealing seat to move upward through the connecting column, so that the conical sealing seat can gradually move away from the conical groove in the liquid inlet tube, so that there is a gap between the conical groove and the sealing seat. Since the bottom end of the extension tube is extended into the blood tumor sample, the extension tube can introduce the sample into the liquid inlet tube through the adapter, and the sample is then introduced into the revolving cylinder through the gap between the conical groove and the sealing seat and the discharge hole;

[0015] The total amount of extraction is controlled by observing the scale bar on the outer wall of the revolving cylinder. When the sample extraction is completed, stop pulling the handle, and support the slide plate with the elastic force of the second spring, so that the slide plate drives the slide column and the connecting column to move downward, and the connecting column drives the sealing seat to move downward and locate in the conical groove. Since the sealing seat is conical in design, it can fit tightly with the conical groove, and the liquid inlet pipe is automatically blocked to prevent the sample in the revolving cylinder from flowing back into the liquid inlet pipe.

[0016] When the sample in the rotating drum is introduced into multiple centrifuge tubes, the handle is pressed down to drive the pull rod and the piston column to move downward. During the downward movement of the piston column, the pressure on the inner wall of the rotating drum can be increased, so that the sliding column can be subjected to the continuous downward pressure, so that the sealing seat below can fit tightly with the conical groove, thereby improving the anti-backflow effect. Since the one-way valve on the conduit is connected to the bottom of the rotating drum, the sample inside the rotating drum can enter the conduit through the one-way valve. The one-way valve can prevent the sample from flowing back into the rotating drum. The sample can enter the centrifuge tube along with the conduit and the stop valve. Multiple one-way valves are connected to the rotating drum. During the downward pressure of the piston column, multiple centrifuge tubes can be filled at the same time, and the samples inside the rotating drum can be diverted through multiple conduits to facilitate quantitative sampling. The stop valve at the bottom of the conduit can be closed or opened, and different numbers of centrifuge tubes can be filled according to needs.

[0017] When centrifuging the sample in the centrifuge tube to separate the supernatant and the lower layer of cells, the extension tube is removed from the adapter, and the bottom bracket assembly is placed in the placement groove in the centrifuge housing through the support plate. The mounting base in the bottom bracket assembly contacts the bottom of the inner wall of the placement groove, and the rectangular groove at the bottom of the mounting base can be embedded in the centrifuge housing structure installed at the bottom of the placement groove, which plays a role in limiting the mounting base. At this time, the support plate overlaps the two sides of the top of the centrifuge housing, and the support plate is fixed to the centrifuge housing by tightening the bolts;

[0018] The centrifuge shell is controlled to work so that it drives the bottom support assembly to rotate, and the bottom support assembly drives the turnover drum and multiple centrifuge tubes to perform centrifugal work. The fixed frame can drive the column to rotate around the hinge seat under the centrifugal effect, so that the multiple fixed frames drive the multiple centrifuge tubes to unfold, and the centrifuge tubes are in an inclined state, which helps the sample particles to slide to the bottom along the tube wall of the centrifuge tube to form a sediment. At the same time, the top of the fixed frame can pull the telescopic rod so that the bottom end of the telescopic rod can rotate around the top of the fixed frame. When the centrifugal work is completed, the telescopic rod is contracted and the fixed frame is pulled to deflect through the pulling action of the first spring, so that the fixed frame drives the centrifuge tube to return to the initial position. When removing the centrifuge tube, the stop valve at the end of the catheter is pulled out to separate the stop valve from the end cover. Next, the centrifuge tube can be pulled upward to be removed from the mounting assembly to facilitate cell extraction.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The present invention pulls the handle upward, so that the handle drives the piston column to move upward through the pull rod. Since the bottom of the revolving cylinder and the piston column are in a sealed state, the volume of the gas in the revolving cylinder changes, so that the extension tube can introduce the sample into the liquid inlet pipe through the adapter, and the sample is then introduced into the revolving cylinder through the gap between the conical groove and the sealing seat and the discharge hole. The total amount of extraction is controlled by observing the scale bar on the outer wall of the revolving cylinder. When the sample extraction work is completed, the handle is stopped, and the slide plate is supported by the elastic force of the second spring, so that the slide plate drives the slide column and the connecting column to move downward, and the connecting column drives the sealing seat to move downward and is located in the conical groove. Since the sealing seat is conical in design, it can fit tightly with the conical groove, and the liquid inlet pipe is automatically blocked to prevent the sample in the revolving cylinder from flowing back into the liquid inlet pipe. , press the handle down to drive the pull rod and the piston column to move downward, the sample inside the revolving cylinder can enter the conduit through the one-way valve, the one-way valve can prevent the sample from flowing back into the revolving cylinder, and the sample can enter the centrifuge tube along the conduit and the stop valve, and multiple one-way valves are connected to the revolving cylinder. During the downward pressure of the piston column, multiple centrifuge tubes can be filled at the same time, and the samples inside the revolving cylinder can be diverted through multiple conduits to facilitate quantitative sampling. The stop valve at the bottom of the conduit can be closed or opened, and different numbers of centrifuge tubes can be filled as needed. Therefore, the total amount of samples is collected at one time and then diverted to multiple centrifuge tubes, which facilitates and quickly completes the quantitative sampling and extraction work, and the samples inside the revolving cylinder will not flow back, preventing cross contamination.

[0021] 2. The present invention controls the centrifuge shell to work so that it drives the bottom support assembly to rotate, and the bottom support assembly drives the turnover drum and multiple centrifuge tubes to perform centrifugal work. The fixed frame can drive the column to rotate around the hinge seat under the centrifugal effect, so that the multiple fixed frames drive the multiple centrifuge tubes to unfold, and the centrifuge tubes are in an inclined state, which helps the sample particles to slide to the bottom along the tube wall of the centrifuge tube to form a sediment. At the same time, the top of the fixed frame can pull the telescopic rod so that the bottom end of the telescopic rod can rotate around the top of the fixed frame. When the centrifugal work is completed, the telescopic rod is contracted and the fixed frame is pulled to deflect through the pulling action of the first spring, so that the fixed frame drives the centrifuge tube to return to the initial position. At this time, the centrifuge tube is in a relatively vertical state, so it will not interfere with the taking and placing of the centrifuge tube. When removing the centrifuge tube, the stop valve at the end of the catheter is pulled out to separate the stop valve from the end cover. Then, the centrifuge tube can be removed from the mounting assembly by pulling it upward, thereby improving work efficiency and operating convenience. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0023] Figure 1 It is a three-dimensional structural schematic diagram of the present invention;

[0024] Figure 2 It is a structural schematic diagram of the cross section of the rotating drum of the present invention;

[0025] Figure 3 It is a structural schematic diagram of the connection between the bottom support assembly and the turnover drum of the present invention;

[0026] Figure 4 It is a structural schematic diagram of the bottom bracket assembly of the present invention;

[0027] Figure 5 It is a structural schematic diagram of the erection assembly of the present invention;

[0028] Figure 6 For the present invention Figure 5 The enlarged structural diagram at A in the middle;

[0029] Figure 7 This is a schematic diagram of the structure of the connection between the liquid inlet pipe and the adapter of the present invention;

[0030] Figure 8 It is a schematic diagram of the structure of the isolation component of the present invention.

[0031] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0032] 1. Centrifuge housing; 2. Placement slot; 3. Support plate; 4. Bolt; 5. Bearing seat; 6. Rotating cylinder; 7. Scale bar; 8. Suction assembly; 801. Piston column; 802. Groove; 803. Pull rod; 804. Handle; 9. Bottom bracket assembly; 901. Mounting base; 902. Rectangular slot; 903. Operation hole; 904. Support frame; 905. Opening; 906. Articulated seat; 10. Erection assembly; 101. Fixing frame; 102. Spacer ; 103, column; 104, telescopic rod; 105, first spring; 11, centrifuge tube; 12, liquid guide assembly; 121, catheter; 122, one-way valve; 123, stop valve; 13, end cover; 14, liquid inlet pipe; 15, adapter; 16, extension pipe; 17, conical groove; 18, isolation assembly; 181, sealing seat; 182, connecting column; 183, slide plate; 184, slide column; 185, second spring; 19, baffle cover; 20, discharge hole. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] See also Figure 1-Figure 8 , the present invention provides a technical solution:

[0035] A rapid extraction device for cell collection includes a centrifuge housing 1, on which a turnover cylinder 6 is installed, and a bottom support assembly 9 is fixedly connected to the bottom of the turnover cylinder 6. A placement groove 2 is provided at the bottom of the centrifuge housing 1, and the bottom of the bottom support assembly 9 is installed in the placement groove 2. The bottom support assembly 9 includes a mounting base 901, and a rectangular groove 902 is provided at the bottom of the mounting base 901. An operating hole 903 is provided at the top of the inner wall of the rectangular groove 902. The bottom of the mounting base 901 is connected to the bottom of the inner wall of the placement groove 2, and a support frame 904 is fixed to the top of the mounting base 901. The support frame 904 is fixed to the bottom of the turnover cylinder 6.

[0036] As a further solution of the present invention, a bearing seat 5 is installed outside the rotating drum 6, and a support plate 3 is clamped outside the bearing seat 5. The support plate 3 is clamped on the top of the centrifuge housing 1. Two bolts 4 are respectively threaded on both sides of the support plate 3, and the support plate 3 is fixed to one side of the centrifuge housing 1 through the bolts 4. The bolts 4 on both sides of the support plate 3 are loosened to release the locking state between the support plate 3 and the centrifuge housing 1, and the support plate 3 is lifted, so as to facilitate the removal of the rotating drum 6 and the bottom support assembly 9 from the centrifuge housing 1. The outer wall of the rotating drum 6 is provided with a scale bar 7, and the total amount of extraction is controlled by observing the scale bar 7 on the outer wall of the rotating drum 6. When working, the bottom support assembly 9 is placed in the placement groove 2 in the centrifuge housing 1 through the support plate 3, and the mounting base 901 in the bottom support assembly 9 is in contact with the bottom of the inner wall of the placement groove 2. The rectangular groove 902 at the bottom of the mounting base 901 can be embedded in the centrifuge housing structure installed at the bottom of the placement groove 2, which plays a role in limiting the mounting base 901 and improving the stability of centrifugal work.

[0037] A plurality of mounting components 10 are installed on the outside of the base component 9, and a centrifuge tube 11 is arranged inside the mounting component 10. An end cap 13 is clamped on the top of the centrifuge tube 11, and a liquid guide component 12 is connected to the end cap 13. The top of the liquid guide component 12 is connected to the bottom of the revolving drum 6. A liquid inlet pipe 14 is installed at the center of the bottom of the revolving drum 6, and a conversion joint 15 is connected to the bottom of the liquid inlet pipe 14. An extension tube 16 is connected to the bottom of the conversion joint 15; a conical groove 17 is provided on the inner wall of the liquid inlet pipe 14, and an isolation component 18 is installed in the conical groove 17. A baffle 19 is provided outside the isolation component 18, and the baffle 19 is fixed to the bottom of the inner wall of the revolving drum 6. A plurality of discharge holes 20 are provided on the outer wall of the baffle 19; by collecting the total amount of the sample at one time and then diverting the sample to multiple centrifuge tubes 11, quantitative sampling and extraction can be completed quickly and conveniently, and the sample inside the revolving drum 6 will not flow back, so cross contamination can be prevented.

[0038] As a further solution of the present invention, a suction assembly 8 is installed in the revolving cylinder 6, and the suction assembly 8 includes a piston column 801, which is located in the revolving cylinder 6, and a groove 802 is provided at the bottom of the piston column 801 corresponding to the position of the baffle 19, and a pull rod 803 is provided at the top of the piston column 801, and the pull rod 803 is connected to the bottom of the revolving cylinder 6 through a limit sleeve, and a handle 804 is fixed to the top of the pull rod 803. By pressing the handle 804 downward, it drives the pull rod 803 and the piston column 801 to move downward, and the pressure on the inner wall of the revolving cylinder 6 can be increased during the downward movement of the piston column 801, and the groove 802 at the bottom of the piston column 801 can move to the outside of the baffle 19, and the bottom of the piston column 801 contacts the bottom of the inner wall of the revolving cylinder 6, so that the sample inside the revolving cylinder 6 can be easily discharged, and the interference of the baffle 19 can be prevented, which makes it difficult to discharge the sample inside the revolving cylinder 6.

[0039] As a further solution of the present invention, the mounting assembly 10 includes a fixed frame 101, which is L-shaped and has two partition bars 102 fixed to the inner wall of the fixed frame 101. The bottom end of the centrifuge tube 11 passes through the two partition bars 102 and is clamped with the bottom of the inner wall of the fixed frame 101. A column 103 is fixed to the bottom of the inner wall of the fixed frame 101. The top of the column 103 passes through the two partition bars 102 and is connected to the hinge seat 906. The top of the fixed frame 101 is hinged with a telescopic rod 104 through a pin shaft. The top of the telescopic rod 104 is hinged to the bottom of the revolving cylinder 6 through a pin shaft, and the outer sleeve of the telescopic rod 104 is connected with a first spring 105.

[0040] The fixing frame 101 can drive the column 103 to rotate around the hinge seat 906 under the centrifugal effect, so that the multiple fixing frames 101 drive the multiple centrifuge tubes 11 to unfold, and the centrifuge tubes 11 are in an inclined state, which helps the sample particles to slide to the bottom along the tube wall of the centrifuge tube 11 to form a sediment; when the centrifugation work is completed, the telescopic rod 104 is contracted and the fixing frame 101 is pulled to deflect, so that the fixing frame 101 drives the centrifuge tube 11 to return to the initial position, and the centrifuge tube 11 is in a relatively vertical state, which is convenient for taking and placing the centrifuge tube 11.

[0041] As a further solution of the present invention, the liquid guiding component 12 includes a conduit 121, a one-way valve 122 is installed at the top of the conduit 121, the one-way valve 122 is connected to the bottom of the revolving cylinder 6, and the bottom end of the conduit 121 is connected to a stop valve 123, and the bottom end of the stop valve 123 is inserted into the top of the end cover 13.

[0042] Because the one-way valve 122 on the conduit 121 is connected to the bottom of the revolving drum 6, the sample inside the revolving drum 6 can enter the conduit 121 through the one-way valve 122. The one-way valve 122 can prevent the sample from flowing back into the revolving drum 6. The sample can enter the centrifuge tube 11 along with the conduit 121 and the stop valve 123. The stop valve 123 can be closed or opened, and different numbers of centrifuge tubes 11 can be filled as needed.

[0043] As a further solution of the present invention, the isolation assembly 18 includes a sealing seat 181, which is conical in design and is clamped in the conical groove 17. A connecting column 182 is fixed to the top of the sealing seat 181. The top of the connecting column 182 passes through the bottom of the block cover 19 and is fixed with a slide plate 183. A sliding column 184 is fixed on the slide plate 183. The sliding column 184 passes through and slides on the block cover 19. A second spring 185 is connected to the outer sleeve of the sliding column 184. The two ends of the second spring 185 are respectively fixed on the slide plate 183 and the top of the inner wall of the block cover 19.

[0044] The slide plate 183 is supported by the elastic force of the second spring 185, so that the slide plate 183 drives the sliding column 184 and the connecting column 182 to move downward, and the connecting column 182 drives the sealing seat 181 to move downward and be located in the conical groove 17. Since the sealing seat 181 is conical in design, it can fit tightly with the conical groove 17, and the liquid inlet pipe 14 is automatically blocked to prevent the sample in the revolving cylinder 6 from flowing back into the liquid inlet pipe 14.

[0045] A method for using a rapid extraction device for cell collection, the method comprising the following steps:

[0046] When collecting and extracting cells from a blood tumor sample, the bolts 4 on both sides of the support plate 3 are loosened to release the locking state between the support plate 3 and the centrifuge housing 1, the support plate 3 is lifted to remove the revolving cylinder 6 and the bottom support assembly 9 from the centrifuge housing 1, and the extension tube 16 is connected to the bottom end of the liquid inlet tube 14 through the adapter 15. Next, the bottom end of the extension tube 16 is extended into the blood tumor sample, and the handle 804 is pulled upward to drive the piston column 801 upward through the pull rod 803. Since the bottom of the revolving cylinder 6 and the piston column 801 are in a sealed state, the volume of the gas in the revolving cylinder 6 changes, thereby The change of gas pressure causes the slide post 184 to be pushed upward, and the slide post 184 drives the slide plate 183 to move upward inside the shield 19. The slide plate 183 drives the sealing seat 181 to move upward through the connecting column 182, so that the conical sealing seat 181 can gradually move away from the conical groove 17 in the liquid inlet pipe 14, so that there is a gap between the conical groove 17 and the sealing seat 181. Because the bottom end of the extension tube 16 extends into the blood tumor sample, the extension tube 16 can introduce the sample into the liquid inlet pipe 14 through the adapter 15, and the sample is then introduced into the turnover cylinder 6 through the gap between the conical groove 17 and the sealing seat 181 and the discharge hole 20;

[0047] The total amount of extraction is controlled by observing the scale bar 7 on the outer wall of the revolving drum 6. When the extraction of the sample is completed, stop pulling the handle 804, and support the slide plate 183 through the elastic force of the second spring 185, so that the slide plate 183 drives the slide column 184 and the connecting column 182 to move downward, and the connecting column 182 drives the sealing seat 181 to move downward and be located in the conical groove 17. Since the sealing seat 181 is conical in design, it can fit tightly with the conical groove 17, and the liquid inlet pipe 14 is automatically blocked to prevent the sample in the revolving drum 6 from flowing back into the liquid inlet pipe 14;

[0048] When the sample in the revolving cylinder 6 is introduced into a plurality of centrifuge tubes 11, the handle 804 is pressed down to drive the pull rod 803 and the piston column 801 to move downward. During the downward movement of the piston column 801, the pressure on the inner wall of the revolving cylinder 6 can be increased, so that the sliding column 184 can be subjected to a continuous downward pressure, so that the sealing seat 181 below can fit tightly with the conical groove 17, thereby improving the anti-backflow effect. Since the one-way valve 122 on the conduit 121 is connected to the bottom of the revolving cylinder 6, the sample inside the revolving cylinder 6 can enter the conduit 121 through the one-way valve 122, and the one-way valve 122 can be used to prevent the sample from entering the conduit 121. The one-way valve 122 can prevent the sample from flowing back into the revolving drum 6. The sample can enter the interior of the centrifuge tube 11 along the conduit 121 and the stop valve 123. The multiple one-way valves 122 are all connected to the revolving drum 6. When the piston rod 801 is pressed down, multiple centrifuge tubes 11 can be filled at the same time, and the samples inside the revolving drum 6 can be diverted through the multiple conduits 121 to complete the quantitative sampling work. The stop valve 123 at the bottom of the conduit 121 can be closed or opened, and different numbers of centrifuge tubes 11 can be filled as needed.

[0049] When the sample in the centrifuge tube 11 is centrifuged to separate the supernatant and the lower layer of cells, the extension tube 16 is removed from the adapter 15, and the bottom bracket assembly 9 is placed in the placement groove 2 in the centrifuge housing 1 through the support plate 3. The mounting base 901 in the bottom bracket assembly 9 contacts the bottom of the inner wall of the placement groove 2, and the rectangular groove 902 at the bottom of the mounting base 901 can be embedded in the centrifuge housing structure installed at the bottom of the placement groove 2, which plays a role in limiting the mounting base 901. At this time, the support plate 3 is overlapped on both sides of the top of the centrifuge housing 1, and the support plate 3 is fixed to the centrifuge housing 1 by tightening the bolts 4;

[0050] The centrifuge housing 1 is controlled to work so that it drives the bottom support assembly 9 to rotate, and the bottom support assembly 9 drives the turnover drum 6 and the plurality of centrifuge tubes 11 to perform centrifugal work. The fixed frame 101 can drive the column 103 to rotate around the hinge seat 906 under the centrifugal effect, so that the plurality of fixed frames 101 drive the plurality of centrifuge tubes 11 to unfold, so that the centrifuge tubes 11 are in an inclined state, which helps the sample particles to slide along the tube wall of the centrifuge tube 11 to form a sediment at the bottom. At the same time, the top of the fixed frame 101 can pull the telescopic rod 104, so that the telescopic rod 104 The bottom end of 04 can rotate around the top of the fixed frame 101. When the centrifugation work is completed, the telescopic rod 104 is contracted and the fixed frame 101 is pulled to deflect through the pulling action of the first spring 105, so that the fixed frame 101 drives the centrifuge tube 11 to return to the initial position. When removing the centrifuge tube 11, the stop valve 123 at the end of the catheter 121 is pulled out to separate the stop valve 123 from the end cover 13. Then, the centrifuge tube 11 can be pulled out upward to be removed from the mounting assembly 10 to facilitate the extraction of cells.

Claims

1. A rapid extraction device for cell collection, comprising a centrifuge housing (1), characterized in that: A rotating drum (6) is installed on the centrifuge housing (1), and a bottom support assembly (9) is fixedly connected to the bottom of the rotating drum (6). A placement groove (2) is provided at the bottom of the centrifuge housing (1), and the bottom of the bottom support assembly (9) is installed in the placement groove (2). A suction assembly (8) is installed in the rotating drum (6), and a plurality of mounting assemblies (10) are installed outside the bottom support assembly (9). A centrifuge tube (11) is arranged in the mounting assembly (10), and an end cover (13) is clamped on the top of the centrifuge tube (11). A liquid guide assembly (12) is connected to the end cover (13), and the guide assembly (12) is connected to the end cover (13). The top of the liquid component (12) is connected to the bottom of the rotating drum (6), a liquid inlet pipe (14) is installed at the center of the bottom of the rotating drum (6), the bottom end of the liquid inlet pipe (14) is connected to an adapter (15), the bottom of the adapter (15) is connected to an extension pipe (16), the inner wall of the liquid inlet pipe (14) is provided with a conical groove (17), an isolation component (18) is installed in the conical groove (17), a baffle (19) is provided outside the isolation component (18), the baffle (19) is fixed to the bottom of the inner wall of the rotating drum (6), and the outer wall of the baffle (19) is provided with a plurality of discharge holes (20).

2. A rapid extraction device for cell collection according to claim 1, characterized in that: A bearing seat (5) is installed outside the rotating drum (6), and a support plate (3) is clamped outside the bearing seat (5). The support plate (3) is clamped on the top of the centrifuge housing (1), and two bolts (4) are respectively threadedly connected on both sides of the support plate (3), and the support plate (3) is fixed to one side of the centrifuge housing (1) by the bolts (4). The outer wall of the rotating drum (6) is provided with a scale bar (7).

3. A rapid extraction device for cell collection according to claim 1, characterized in that: The suction assembly (8) includes a piston column (801), and the piston column (801) is located in the revolving cylinder (6). A groove (802) is provided at the bottom of the piston column (801) corresponding to the position of the baffle (19). A pull rod (803) is provided at the top of the piston column (801). The pull rod (803) is connected to the bottom of the revolving cylinder (6) through a limiting sleeve, and a handle (804) is fixed to the top of the pull rod (803).

4. A rapid extraction device for cell collection according to claim 1, characterized in that: The base support assembly (9) comprises a mounting base (901), the bottom of the mounting base (901) is provided with a rectangular groove (902), the top of the inner wall of the rectangular groove (902) is provided with an operating hole (903), the bottom of the mounting base (901) is connected to the bottom of the inner wall of the placement groove (2), the top of the mounting base (901) is fixed with a support frame (904), the support frame (904) is fixed to the bottom of the turnover cylinder (6), the support frame (904) is provided with openings (905) at positions corresponding to the mounting assembly (10), the top of the inner wall of the opening (905) is provided with a hinge seat (906), and the top of the mounting assembly (10) is hinged to the support frame (904) through the hinge seat (906).

5. A rapid extraction device for cell collection according to claim 4, characterized in that: The mounting assembly (10) comprises a fixed frame (101), the fixed frame (101) is L-shaped, and two partition bars (102) are fixed to the inner wall of the fixed frame (101), the bottom end of the centrifuge tube (11) passes through the two partition bars (102) and is clamped with the bottom of the inner wall of the fixed frame (101), a column (103) is fixed to the bottom of the inner wall of the fixed frame (101), the top end of the column (103) passes through the two partition bars (102) and is connected to the hinge seat (906), the top end of the fixed frame (101) is hinged with a telescopic rod (104) through a pin shaft, the top end of the telescopic rod (104) is hinged with the bottom of the rotating cylinder (6) through a pin shaft, and the outer shell of the telescopic rod (104) is connected with a first spring (105).

6. A rapid extraction device for cell collection according to claim 1, characterized in that: The liquid guiding assembly (12) comprises a conduit (121), a one-way valve (122) being installed at the top end of the conduit (121), the one-way valve (122) being communicated with the bottom of the revolving drum (6), the bottom end of the conduit (121) being connected to a stop valve (123), the bottom end of the stop valve (123) being plugged into the top of the end cover (13).

7. A rapid extraction device for cell collection according to claim 1, characterized in that: The isolation assembly (18) includes a sealing seat (181), which is conical in design and is clamped in the conical groove (17). A connecting column (182) is fixed to the top of the sealing seat (181). The top of the connecting column (182) passes through the bottom of the baffle cover (19) and is fixed with a slide plate (183). A sliding column (184) is fixed on the slide plate (183). The sliding column (184) passes through and slides on the baffle cover (19). A second spring (185) is connected to the outer shell of the sliding column (184). The two ends of the second spring (185) are respectively fixed on the slide plate (183) and the top of the inner wall of the baffle cover (19).

8. A method for using a rapid extraction device for cell collection, according to any one of claims 1 to 7, characterized in that: The method of use comprises the following steps: When collecting and extracting cells from a blood tumor sample, the bolts (4) on both sides of the support plate (3) are loosened to release the locking state between the support plate (3) and the centrifuge housing (1), the support plate (3) is lifted to remove the revolving cylinder (6) and the bottom support assembly (9) from the centrifuge housing (1), the extension tube (16) is connected to the bottom end of the liquid inlet tube (14) through the adapter (15), and then the bottom end of the extension tube (16) is inserted into the blood tumor sample, and the handle (804) is pulled upward, so that the handle (804) drives the piston column (801) to move upward through the pull rod (803). Since the bottom of the revolving cylinder (6) and the piston column (801) are in a sealed state, the volume of the gas in the revolving cylinder (6) changes, thereby The change in gas pressure causes the slide column (184) to be pushed upward, and the slide column (184) drives the slide plate (183) to move upward inside the shield (19). The slide plate (183) drives the sealing seat (181) to move upward through the connecting column (182), so that the conical sealing seat (181) can gradually move away from the conical groove (17) in the liquid inlet pipe (14), so that there is a gap between the conical groove (17) and the sealing seat (181). Because the bottom end of the extension tube (16) extends into the blood tumor sample, the extension tube (16) can introduce the sample into the liquid inlet pipe (14) through the adapter (15), and the sample is then introduced into the rotating drum (6) through the gap between the conical groove (17) and the sealing seat (181) and the discharge hole (20); The total amount of extraction is controlled by observing the scale bar (7) on the outer wall of the revolving drum (6). When the extraction of the sample is completed, the handle (804) is stopped from being pulled, and the slide plate (183) is supported by the elastic force of the second spring (185), so that the slide plate (183) drives the slide column (184) and the connecting column (182) to move downward, and the connecting column (182) drives the sealing seat (181) to move downward and be located in the conical groove (17). Since the sealing seat (181) is conical in design, it can fit tightly with the conical groove (17), and the liquid inlet pipe (14) is automatically blocked to prevent the sample in the revolving drum (6) from flowing back into the liquid inlet pipe (14); When the sample in the revolving cylinder (6) is introduced into a plurality of centrifuge tubes (11), the handle (804) is pressed downward to drive the pull rod (803) and the piston column (801) to move downward. During the downward movement of the piston column (801), the pressure on the inner wall of the revolving cylinder (6) can be increased, so that the sliding column (184) can be subjected to a continuous downward pressure, so that the sealing seat (181) below can fit tightly with the conical groove (17), thereby improving the anti-backflow effect. Since the one-way valve (122) on the conduit (121) is connected to the bottom of the revolving cylinder (6), the sample inside the revolving cylinder (6) can pass through the one-way valve (122) and enter the conduit (121). The one-way valve (122) can prevent the sample from flowing back into the revolving cylinder (6). The sample can enter the interior of the centrifuge tube (11) along with the conduit (121) and the stop valve (123). The multiple one-way valves (122) are all connected to the revolving cylinder (6). During the downward pressing of the piston column (801), the multiple centrifuge tubes (11) can be filled at the same time. Moreover, the samples in the revolving cylinder (6) can be diverted through the multiple conduits (121) to facilitate quantitative sampling. The stop valve (123) at the bottom of the conduit (121) can be closed or opened, so that different numbers of centrifuge tubes (11) can be filled as needed. When the sample in the centrifuge tube (11) is centrifuged to separate the supernatant and the lower layer of cells, the extension tube (16) is removed from the adapter (15), and the base assembly (9) is placed in the placement groove (2) in the centrifuge housing (1) through the support plate (3). The mounting base (901) in the base assembly (9) contacts the bottom of the inner wall of the placement groove (2), and the rectangular groove (902) at the bottom of the mounting base (901) can be embedded in the centrifuge housing structure installed at the bottom of the placement groove (2), playing a role in limiting the mounting base (901). At this time, the support plate (3) is overlapped on both sides of the top of the centrifuge housing (1), and the support plate (3) is fixed to the centrifuge housing (1) by tightening the bolts (4); The centrifuge housing (1) is controlled to work so as to drive the bottom support assembly (9) to rotate, and the bottom support assembly (9) drives the turnover drum (6) and the plurality of centrifuge tubes (11) to perform centrifugal work. The fixed frame (101) can drive the upright column (103) to rotate around the hinge seat (906) under the centrifugal action, so that the plurality of fixed frames (101) drive the plurality of centrifuge tubes (11) to unfold, so that the centrifuge tubes (11) are in an inclined state, which helps the sample particles to slide along the tube wall of the centrifuge tube (11) to the bottom to form a sediment. At the same time, the top of the fixed frame (101) can pull the telescopic rod (104) so ​​that the telescopic rod (104) The bottom end of the telescopic rod (104) can rotate around the top of the fixing frame (101). When the centrifugation work is completed, the first spring (105) pulls the telescopic rod (104) to contract and pull the fixing frame (101) to deflect, so that the fixing frame (101) drives the centrifuge tube (11) to return to the initial position. When removing the centrifuge tube (11), the stop valve (123) at the end of the catheter (121) is pulled out to separate the stop valve (123) from the end cover (13). Then, the centrifuge tube (11) can be pulled out upward to be removed from the mounting assembly (10) to facilitate the extraction of cells.

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