Application of tartary buckwheat antioxidant peptide in preparation of weight-reducing medicine

Abstract

The invention provides an application of a tartary buckwheat antioxidant peptide in preparation of weight-reducing drugs, the tartary buckwheat antioxidant peptide (SEQ ID NO: 1) is used for reducing triglyceride and total cholesterol levels in serum and reducing body weight, and the tartary buckwheat antioxidant peptide is used for preparation of drugs for reducing blood fat and preparation of weight-reducing nourishment or drugs. The tartary buckwheat antioxidant peptide achieves the purpose of losing weight by reducing body weight and blood fat, and a new example is provided for the development of food-borne weight-losing nourishments or medicines and the clinical application of the tartary buckwheat antioxidant peptide.

Description

Technical Field

[0001] The present invention belongs to the technical field of the application of tartary buckwheat antioxidant peptides, and particularly relates to the application of a tartary buckwheat antioxidant peptide in the preparation of weight loss drugs. Background Art

[0002] With the increase in the obese population and the enhancement of health awareness, consumers' demand for weight loss products is continuously expanding. In 2022, the global obese population exceeded one billion, accounting for about one-eighth of the global total population. It is estimated that by 2035, more than 4 billion people globally will be obese or overweight, accounting for 51% of the global total population. Therefore, it is of great significance to develop weight loss nutritional products or drugs with small side effects (such as those from food sources). Summary of the Invention

[0003] The technical problem to be solved by the present invention is to provide an application of a tartary buckwheat antioxidant peptide in the preparation of weight loss drugs in view of the above-mentioned deficiencies of the prior art. This tartary buckwheat antioxidant peptide can be used to prepare weight loss nutritional products or drugs for reducing body weight and lowering blood lipid, and has broad clinical application prospects.

[0004] To solve the above technical problem, the technical solution adopted by the present invention is: an application of a tartary buckwheat antioxidant peptide in the preparation of weight loss drugs, wherein the tartary buckwheat antioxidant peptide is used to reduce the levels of triglyceride and total cholesterol in serum, to reduce body weight, the tartary buckwheat antioxidant peptide is used in the preparation of blood lipid-lowering drugs, and in the preparation of weight loss nutritional products or drugs; the amino acid sequence of the tartary buckwheat antioxidant peptide is as shown in SEQ ID NO:1.

[0005] Preferably, the weight loss nutritional product or drug is a pharmaceutically acceptable carrier and excipient.

[0006] Preferably, the weight loss nutritional product or drug contains one or more pharmaceutically acceptable carriers including the amino acid sequence of the tartary buckwheat antioxidant peptide.

[0007] Preferably, using the tartary buckwheat antioxidant peptide as an active ingredient, it is made into any dosage form of tablets, capsules, powders, mixtures, pills, sprays, granules, capsules, oral liquids, injections.

[0008] The present invention has the following advantages compared with the prior art:

[0009] The tartary buckwheat antioxidant peptide (SEQ ID NO:1) of the present invention achieves the purpose of weight loss by reducing body weight and lowering blood lipid. The present invention provides a new example for the development of food-derived weight loss nutritional products or drugs and the clinical application of tartary buckwheat antioxidant peptides.

[0010] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Brief Description of the Drawings

[0011] Figure 1 In the high-fat diet mice of Example 2 of the present invention, the tartary buckwheat antioxidant peptide AFYRW alleviates the body weight of high-fat diet mice graph.

[0012] Figure 2 In the high-fat diet mice of Example 2 of the present invention, the tartary buckwheat antioxidant peptide AFYRW improves its serum triglyceride (TG) graph.

[0013] Figure 3 In the high-fat diet mice of Example 2 of the present invention, the tartary buckwheat antioxidant peptide AFYRW improves its serum total cholesterol (TC) graph. Detailed implementation mode

[0014] Example 1

[0015] The preparation method of the tartary buckwheat antioxidant peptide (SEQ ID NO: 1) in the present invention can be found in Chinese Patent CN109336953B. The tartary buckwheat antioxidant peptide in the present invention is the tartary buckwheat antioxidant peptide P3 prepared in Chinese Patent CN109336953B. In the present invention, this tartary buckwheat antioxidant peptide is named tartary buckwheat antioxidant peptide AFYRW.

[0016] The application of the tartary buckwheat antioxidant peptide AFYRW as a weight loss nutritional product or medicine. Example 2 is an in vivo experiment on the tartary buckwheat antioxidant peptide AFYRW to reduce body weight and lower blood lipids.

[0017] The tartary buckwheat antioxidant peptide AFYRW is used to prepare a weight loss nutritional product or medicine, and the weight loss nutritional product or medicine is a pharmaceutically acceptable carrier and excipient.

[0018] The tartary buckwheat antioxidant peptide is used to prepare a weight loss nutritional product or medicine; the weight loss nutritional product or medicine contains one or more pharmaceutically acceptable carriers including the amino acid sequence of the tartary buckwheat antioxidant peptide.

[0019] Using the tartary buckwheat antioxidant peptide AFYRW as the active ingredient, it is made into any dosage form of tablets, capsules, powders, mixtures, pills, sprays, granules, capsules, oral liquids, injections.

[0020] Example 2

[0021] I. Effect of the tartary buckwheat antioxidant peptide AFYRW on the body weight of high-fat diet mice

[0022] Male C57BL / 6 mice are from the Animal Experiment Center of Guizhou Medical University. All animals are housed in a specific pathogen-free (SPF) environment at a temperature of 22 ± 2 °C and a light / dark cycle of 12 hours.

[0023] The experiment is divided into three groups.

[0024] ND: Control mice were fed a normal diet.

[0025] HFD: High-fat diet mice were fed a high-fat diet containing 21% lard and 0.5% cholesterol for 25 consecutive weeks.

[0026] HFD+AFYRW: High-fat diet mice + Tartary buckwheat antioxidant peptide AFYRW (HFD+AFYRW) were fed a high-fat diet containing 21% lard and 0.5% cholesterol, and at the same time, intraperitoneally injected with Tartary buckwheat antioxidant peptide AFYRW (80 mg / kg / d) for 25 consecutive weeks.

[0027] During this period, the body weight of the mice was measured daily. As Figure 1 shown, compared with the control mice (NC), the body weight of the high-fat diet mice (HFD) increased significantly, while the body weight of the high-fat diet mice + Tartary buckwheat antioxidant peptide AFYRW (HFD+AFYRW) decreased significantly. This result indicates that Tartary buckwheat antioxidant peptide AFYRW can reduce the weight gain caused by a high-fat diet.

[0028] II. Tartary buckwheat antioxidant peptide AFYRW reduces blood lipid levels in high-fat diet mice

[0029] Male C57BL / 6 mice were obtained from the Animal Experiment Center of Guizhou Medical University. All animals were housed in a specific pathogen-free (SPF) environment at a temperature of 22±2 °C and a light / dark cycle of 12 hours.

[0030] The experiment was divided into three groups.

[0031] ND: Control mice were fed a normal diet.

[0032] HFD: High-fat diet mice were fed a high-fat diet containing 21% lard and 0.5% cholesterol for 25 consecutive weeks.

[0033] HFD+AFYRW: High-fat diet mice + Tartary buckwheat antioxidant peptide AFYRW (HFD+AFYRW) were fed a high-fat diet containing 21% lard and 0.5% cholesterol, and at the same time, intraperitoneally injected with Tartary buckwheat antioxidant peptide AFYRW (80 mg / kg / d) for 25 consecutive weeks.

[0034] Mouse serum samples were taken to detect triglyceride and total cholesterol levels. As Figure 2 and Figure 3 shown, the serum triglyceride (TG) and total cholesterol (TC) levels of the high-fat diet mice (HFD) were significantly increased, while Tartary buckwheat antioxidant peptide AFYRW (HFD+AFYRW) could down-regulate their serum triglyceride (TG) and total cholesterol (TC) levels.

[0035] In summary, the tartary buckwheat antioxidant peptide AFYRW in the present invention can be used to reduce the levels of triglyceride and total cholesterol in serum, to reduce body weight, and is used for the preparation of drugs for reducing blood lipid and for the preparation of weight loss nutritional products or drugs.

[0036] As described above, it is only a preferred embodiment of the present invention and does not impose any limitation on the present invention. Any simple modification, change and equivalent change made to the above embodiments according to the technical essence of the invention still fall within the protection scope of the technical solution of the present invention.

Claims

1. An application of tartary buckwheat antioxidant peptide in the preparation of weight loss drugs, characterized in that: The tartary buckwheat antioxidant peptide is used to reduce the levels of triglycerides and total cholesterol in serum and to reduce body weight. The tartary buckwheat antioxidant peptide is used to prepare lipid-lowering drugs and to prepare weight-loss nutritional products or medicines. The amino acid sequence of the tartary buckwheat antioxidant peptide is shown in SEQ ID NO:

1.

2. The use of a tartary buckwheat antioxidant peptide in the preparation of a weight loss drug according to claim 1, characterized in that: The weight loss nutritional product or medicine is a pharmaceutically acceptable carrier and excipient.

3. The use of a tartary buckwheat antioxidant peptide in the preparation of a weight loss drug according to claim 1, characterized in that: The weight-loss nutritional product or medicine contains one or more pharmaceutically acceptable carriers containing the amino acid sequence of tartary buckwheat antioxidant peptide.

4. Use of a tartary buckwheat antioxidant peptide according to any one of claims 1 to 3 in the preparation of a weight loss drug, characterized in that: The tartary buckwheat antioxidant peptide is used as an active ingredient to prepare any dosage form of tablets, capsules, powders, mixtures, pills, sprays, granules, capsules, oral liquids and injections.

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