A bactericidal composition containing a triazole compound and use thereof

Abstract

The present application relates to a kind of triazole-containing fungicidal composition and its application, comprising active ingredient A and active ingredient B, the active ingredient A is the compound shown in formula (I), active ingredient B is triazole compound, the mass ratio of active ingredient A and active ingredient B is 1:35~36:1.The fungicidal composition of the present application has high antibacterial activity under certain mass ratio, has obvious synergistic effect on wheat scab, has the effect of increasing yield and protecting harvest.

Description

Technical Field

[0001] This invention relates to the field of pesticides and fungicides, specifically to a fungicide composition containing triazole compounds and its application. Background Technology

[0002] Compound (I) is a methoxyacrylate bactericide developed by Sumitomo Chemical Co., Ltd., CAS Registry Number: 2454319-63-0, Chinese chemical name: (2Z)-3-methoxy-2-[(4-methyl[1,1'-biphenyl]-3-yl)oxy]prop-2-enoic acid methyl ester, and its chemical structure is as follows:

[0003]

[0004] Fusarium graminearum, which infects wheat and causes Fusarium head blight, is a significant disease in wheat production. However, due to the lack of stable, high-yielding, and disease-resistant varieties, and the advantages of rapid, efficient, and low-cost chemical control, current methods primarily rely on chemical pesticides for prevention and control. However, due to prolonged, excessive, and unscientific application of fungicides to wheat, Fusarium graminearum has developed varying degrees of resistance to multiple fungicides, including triazole fungicides.

[0005] The application of pesticides is an important means of preventing and controlling plant diseases. This invention has conducted in-depth research on the application of compound (I) to wheat scab. A large number of experimental results show that compound (I) combined with triazole fungicides, especially with propiconazole, chlorfenapyr, tebuconazole, flutriafol, and tebuconazole, can effectively improve the control effect under certain mass ratios and has a significant synergistic effect, which is of great significance for ensuring the safe production of wheat. Summary of the Invention

[0006] Based on the above, the present invention aims to provide a fungicide composition and its formulation containing triazole compounds, mainly used for the prevention and control of plant fungal diseases. The composition or its formulation can enhance efficacy, reduce dosage, prolong the duration of action, delay the development of resistance, and has high safety for crops.

[0007] This objective is achieved through the following technical solution: a bactericidal composition containing triazole compounds, comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), with the following chemical structural formula: Active ingredient B is a triazole compound, which is selected from any one of propiconazole, chlorfenapyr, tebuconazole, flutriafol, and tebuconazole.

[0008] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:35 to 36:1;

[0009] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:32 to 30:1 or any value between the above values;

[0010] Furthermore, the mass ratio of the compound of formula (I) to propiconazole is 1:24 to 24:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to propiconazole is 1:24 to 16:1;

[0012] Furthermore, the mass ratio of the compound of formula (I) to propiconazole is 1:24, 1:16, 2:13, 5:2, 13:2, or 16:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to chlorfluazuron is 1:20 to 30:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to chlorfluazuron is 1:20 to 20:1;

[0015] Furthermore, the mass ratio of the compound of formula (I) to chlorfluazuron is 1:20, 1:12, 1:6, 6:1, 12:1, or 20:1;

[0016] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:24 to 22:1;

[0017] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:24 to 16:1;

[0018] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:24, 1:8, 1:2, 2:1, 8:1, or 16:1;

[0019] Furthermore, the mass ratio of the compound of formula (I) to flutriafol is 1:32 to 24:1;

[0020] Furthermore, the mass ratio of the compound of formula (I) to flutriafol is 1:18 to 14:1;

[0021] Furthermore, the mass ratio of the compound of formula (I) to flutriafol is 1:18, 1:7, 3:2, 7:1, or 14:1;

[0022] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:26 to 21:1;

[0023] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:17 to 17:1;

[0024] Furthermore, the mass ratio of the compound of formula (I) to tebuconazole is 1:17, 1:7, 2:1, 7:1, or 17:1;

[0025] Furthermore, based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 90 wt%.

[0026] Furthermore, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is preferably 5 to 80 wt%.

[0027] Furthermore, the bactericidal composition, in addition to containing the active ingredient, also contains agriculturally permissible auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, or carriers.

[0028] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or

[0029] Further, the dispersant is selected from one or more of the following: lignin sulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0030] Further, the emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0031] Furthermore, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0032] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0033] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0034] Furthermore, the defoamer is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0035] Further, the solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil (soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives, and water; and / or

[0036] Furthermore, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0037] Further, the stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-methylphenol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0038] Furthermore, the synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0039] Furthermore, the carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0040] Furthermore, the dosage form of the bactericidal composition is selected from solid dosage forms and / or liquid dosage forms and / or seed treatment formulations;

[0041] The solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules.

[0042] The liquid formulations include soluble agents, colloids, oils, spreading oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipids, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions.

[0043] Furthermore, the bactericidal composition can be prepared into a pesticide-acceptable formulation, wherein the formulation is a microemulsion, water-in-oil emulsion, suspension, dispersible oil suspension, soluble concentrate, emulsifiable concentrate, suspension emulsion, microcapsule suspension, water-dispersible granules, wettable powder, granules, seed treatment suspension, or seed treatment dry powder.

[0044] Furthermore, the formulation is a microemulsion, emulsifiable concentrate, suspension concentrate, water emulsion, water-dispersible granules, or seed treatment suspension.

[0045] The present invention also discloses the application of the bactericidal composition described above in the prevention and control of plant diseases;

[0046] Furthermore, the plant disease is a plant disease caused by fungi or bacteria;

[0047] Furthermore, the plant disease is a plant disease caused by fungi;

[0048] Furthermore, the plant diseases caused by the fungus are wheat scab, wheat leaf rust, and wheat powdery mildew. The beneficial effects of this invention are:

[0049] 1) The bactericidal composition of the present invention has a synergistic effect in preventing and controlling diseases under certain mass ratios;

[0050] 2) The bactericidal composition of the present invention can reduce the amount of each single agent used, thereby reducing pesticide costs;

[0051] 3) The bactericidal composition and its formulation of the present invention are environmentally friendly and have high safety. Detailed Implementation

[0052] To make the technical solution, objectives and advantages of the present invention clearer, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0053] Indoor toxicity testing:

[0054] Indoor bioassay for wheat scab

[0055] Test basis: NY / T 1156.2-2006 "Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 2: Plate Method for Inhibition of Mycelial Growth of Pathogenic Fungi"; NY / T 1156.6-2006 "Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 6: Determination of Combined Effects of Mixtures".

[0056] Tested pathogen: Wheat scab (Fusarium graminearum);

[0057] Test reagents: propiconazole, chlorfenapyr, tebuconazole, flutriafol, and tebuconazole. All of the above test reagents were provided by the Group's R&D Center.

[0058] Reagent preparation: Dissolve the test drug in acetone first, then dilute it with 0.1% Tween 80 aqueous solution to prepare single-agent stock solutions, and set 5 series of mass concentrations according to the purpose of mixing and the drug activity.

[0059] Melt the PDA medium in a microwave oven and cool it to about 50°C. Following the principle of starting from low concentration and gradually increasing the concentration, take 1 mL of the prepared test solution and 9 mL of PDA medium and add them to a 9 cm diameter petri dish. Mix well to prepare a plate containing the drug at the corresponding concentration.

[0060] Inoculation: Under aseptic conditions, the activated pathogenic fungus was punched into a fungal cake using a 6mm diameter punch. After the drug-containing culture medium solidified, the fungal cake was placed in the center of the culture medium. Finally, the culture dish was sealed with sealing film and placed in an incubator at 27°C and 70% relative humidity for cultivation. A blank control without the drug was set up. Each treatment was repeated 3 times.

[0061] Investigation: The growth of pathogenic fungal hyphae was investigated based on the growth of hyphae in blank control culture dishes. The diameter of colonies was measured with calipers in millimeters (mm). The diameter of each colony was measured vertically once using the cross-sectional method, and the average value was taken.

[0062] Calculation method: Based on the survey results, the mycelial growth inhibition rate is calculated according to the following formula, in percentage (%), and the calculation result is rounded to two decimal places.

[0063] D = D1 - D2

[0064] In the formula:

[0065] D – Colony growth diameter;

[0066] D1—colony diameter;

[0067] D2 – Diameter of the mushroom cake.

[0068]

[0069] I – Mycelial growth inhibition rate;

[0070] D0—Correlation diameter of the blank control group;

[0071] D t — Diameter of colonies grown after chemical treatment.

[0072] Experimental statistics: Data were processed using probability value analysis. The DPS statistical analysis system was used to analyze the data and determine the toxicity regression line and EC50. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0073] Sun Yunpei's method: The synergistic effect of drug mixtures is evaluated based on the co-toxicity coefficient (CTC). A CTC ≥ 120 indicates a synergistic effect; a CTC ≤ 80 indicates an antagonistic effect; and a CTC < 120 indicates an additive effect.

[0074] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0075]

[0076] In the formula:

[0077] ATI – Actual Measured Toxicity Index of Mixtures;

[0078] S – EC of standard bactericides 50 The unit is milligrams per liter (mg / L);

[0079] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0080] TTI = TI A *P A +TI B *P B

[0081] In the formula:

[0082] TTI – Theoretical Toxicity Index of Mixtures;

[0083] TI A —A. Toxicity index of drug A;

[0084] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0085] TI B —Toxicity index of drug B;

[0086] P B—Percentage content of agent B in the mixture, expressed as percentage (%).

[0087]

[0088] In the formula:

[0089] CTC – Cotoxicity Coefficient;

[0090] ATI – Actual Measured Toxicity Index of Mixtures;

[0091] TTI – Theoretical Toxicity Index of Mixtures.

[0092] Experimental results:

[0093] Table 1 shows the results of propiconazole control of wheat scab EC. 50 The concentration was 0.201 mg / L. Compound (I) exhibited additive or synergistic effects when mixed with propiconazole in a mass ratio of 1:35 to 24:1. Specifically, when the mass ratio was 1:24 to 24:1, the co-toxicity coefficient was >120, demonstrating a synergistic effect. The co-toxicity coefficient of compound (I) with propiconazole at a ratio of 5:2 was the highest, at 246.068, EC50. 50 The concentration was 0.188 mg / L, indicating a significant synergistic effect.

[0094] Table 1 shows the indoor assay results of different ratios of compound (I) to propiconazole against wheat scab.

[0095]

[0096] Table 2 shows the results of chlorfenapyr control of wheat scab EC. 50 The concentration was 0.406 mg / L. Compound (I) exhibited a synergistic effect when mixed with chlorfluazuron at a mass ratio of 1:20 to 30:1. Among these, the co-toxicity coefficient was >130 at mass ratios of 1:20 to 20:1, indicating a significant synergistic effect. The co-toxicity coefficient of compound (I) with chlorfluazuron at a ratio of 1:6 was the highest, at 187.529, EC50. 50 The concentration was 0.236 mg / L, indicating a significant synergistic effect.

[0097] Table 2 shows the indoor test results of different ratios of compound (I) with chlorfluazuron against wheat scab.

[0098]

[0099]

[0100] Table 3 shows the results of the control of wheat scab EC with tebuconazole. 50The concentration was 0.758 mg / L. Compound (I) exhibited additive or synergistic effects when mixed with tebuconazole at a mass ratio of 1:33 to 22:1. Specifically, at mass ratios of 1:24 to 22:1, the co-toxicity coefficient was >120, demonstrating a synergistic effect. The co-toxicity coefficient of compound (I) with tebuconazole at a ratio of 1:2 was the highest, at 222.053, EC50. 50 The concentration was 0.367 mg / L, indicating a significant synergistic effect.

[0101] Table 3 shows the indoor test results of different ratios of compound (I) with tebuconazole against wheat scab.

[0102]

[0103]

[0104] Table 4 shows the results of the control of wheat scab EC with flutriafol. 50 The concentration was 0.394 mg / L. Compound (I) exhibited additive or synergistic effects when mixed with flutriafol in a mass ratio of 1:32 to 36:1. Specifically, when the mass ratio was 1:32 to 24:1, the co-toxicity coefficient was >120, demonstrating a synergistic effect. The co-toxicity coefficient of compound (I) with flutriafol at a ratio of 3:2 was the highest, at 183.270, EC50. 50 The concentration was 0.332 mg / L, indicating a significant synergistic effect.

[0105] Table 4 shows the indoor assay results of different ratios of compound (I) to flutriafol against wheat scab.

[0106]

[0107] Table 5 shows the results of the control of wheat scab EC with tebuconazole. 50 The concentration was 0.195 mg / L. Compound (I) exhibited a synergistic effect when mixed with tebuconazole at a mass ratio of 1:26 to 21:1. Specifically, at mass ratios of 1:17 to 17:1, the co-toxicity coefficient was >150, indicating a significant synergistic effect. The co-toxicity coefficient of compound (I) with tebuconazole at a ratio of 2:1 was the highest, at 221.282, EC50. 50 The concentration was 0.189 mg / L, indicating a significant synergistic effect.

[0108] Table 5 shows the indoor test results of different ratios of compound (I) to tebuconazole against wheat scab.

[0109]

[0110] Formulation Examples

[0111] Preparation Example 1: 40% propiconazole emulsifiable concentrate of formula (I) (29:11)

[0112] Formula: 29% of compound (Ⅰ), 11% of propiconazole, 1.2% of BHT, 10% of propylene carbonate, 8% of DMF, 5% of dimethyl sulfoxide, 4% of cyclohexanone, 3% of calcium dodecylbenzenesulfonate, 12% of fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance;

[0113] Preparation method: Add the active ingredients to the cosolvent according to the formulation ratio of the example, and add surfactants and other functional additives thereto. Stir and mix evenly in a stirring mixing tank to obtain emulsifiable oil.

[0114] Preparation Example 2: 30% Formula (I) compound·tebuconazole water emulsion (10:20)

[0115] Formula: 10% of compound (Ⅰ), 20% of tebuconazole, 12% of thiamethoxam, 10% of cyclohexanone, 1% of calcium dodecylbenzenesulfonate, 0.5% of sodium fatty alcohol polyoxyethylene ether sulfate, 5% of EO / PO block copolymer, 5% of glycerol, 1.5% of glycerin, 0.1% of silicone defoamer, 0.15% of xanthan gum, 0.1% of sodium benzoate, and deionized water to make up the balance;

[0116] Preparation method: According to the formulation ratio in the example, the active ingredient is dissolved in the solvent and an emulsifier is added to form a homogeneous oil phase. Deionized water, antifreeze and additives are mixed together to form a homogeneous aqueous phase. Under high-speed shearing, the aqueous phase is added to the oil phase to form a well-dispersed water-emulsion formulation.

[0117] Preparation Example 3: 26% propiconazole aqueous emulsion of formula (I) (16:10)

[0118] Preparation formula: 16% of compound (Ⅰ), 10% propiconazole, 12% thiol, 10% cyclohexanone, 1% calcium dodecylbenzenesulfonate, 0.5% sodium fatty alcohol polyoxyethylene ether sulfate, 5% EO / PO block copolymer, 5% glycerol, 2% glycerol, 0.1% silicone defoamer, 0.2% xanthan gum, 0.1% sodium benzoate, and deionized water to make up the balance;

[0119] Preparation method: Same as in preparation example 2.

[0120] Preparation Example 4: 24% of compound (I) chlorfluazuron emulsifiable concentrate (4:20)

[0121] Preparation formula: 4% of compound (Ⅰ), 20% of chlorfluazuron, 1.5% of BHT, 10% of propylene carbonate, 15% of DMF, 5% of decylamide, 3.5% of calcium dodecylbenzenesulfonate, 10% of fatty alcohol polyoxyethylene ether, and methyl oleate to make up the balance;

[0122] Preparation method: Same as in preparation example 1.

[0123] Preparation Example 5: 42% of Formula (I) compound·chlorfenapyr suspension (6:36)

[0124] Formulation: Compound (I) 6%, chlorfluazuron 36%, polyether 4%, phenethylphenol polyether phosphate salt 3%, sodium dioctyl succinate sulfonate 3%, sodium polycarboxylate 1%, magnesium aluminum silicate 0.5%, xanthan gum 0.2%, glycerol 5%, methylisothiazolinone 0.15%, organosilicon defoamer 0.5%, deionized water to make up the balance;

[0125] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension.

[0126] Preparation Example 6: 25% Formula (I) compound·tebuconazole suspension (12:13)

[0127] Formulation: 12% of compound (I), 13% of tebuconazole, 4% of polyether, 3% of phenethylphenol polyether phosphate salt, 3% of naphthalene sulfonate formaldehyde condensate, 1% magnesium aluminum silicate, 0.2% xanthan gum, 0.5% fumed silica, 0.5% polyvinylpyrrolidone, 5% ethylene glycol, 0.15% methylisothiazolinone, 0.5% silicone oil, and deionized water to make up the balance;

[0128] Preparation method: Same as in preparation example 5.

[0129] Preparation Example 7: 30% of Formula (I) compound·fluconazole suspension (18:12)

[0130] Formulation: 18% of compound (Ⅰ), 12% of flutriafol, 5% of polyether, 3% of sodium dioctyl succinate sulfonate, 3% of styrene-phenol polyoxyethylene ether phosphate, 1% of sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.25% of xanthan gum, 5% of glycerin, 0.15% of methylisothiazolinone, 0.5% of organosilicon defoamer, and deionized water to make up the balance;

[0131] Preparation method: Same as in preparation example 5.

[0132] Preparation Example 8: 18% Formula (I) compound·Iprodione suspension (12:6)

[0133] Formulation: 12% of compound (I), 6% of tebuconazole, 4% of polyether, 3% of phenethylphenol polyether phosphate, 3% of sodium dioctyl succinate sulfonate, 1.5% of sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.3% of xanthan gum, 5% of ethylene glycol, 0.15% of methylisothiazolinone, 0.5% of silicone oil, and deionized water to make up the balance;

[0134] Preparation method: Same as in preparation example 5.

[0135] Preparation Example 9: 60% of formula (I) compound·fluconazole water-dispersible granules (36:24)

[0136] Formulation: 36% of compound (I), 24% of flutriafol, 3% of sodium dodecyl sulfate, 8% of naphthalene sulfonate formaldehyde condensate, 1% of sodium fatty alcohol polyoxyethylene ether sulfate, 2% of sodium polycarboxylate, 6.5% of sodium lignosulfonate, 5% of attapulgite, and the balance is made up with kaolin.

[0137] Preparation method: According to the formula ratio, add the active ingredients to the carrier, and add surfactants and other functional additives to it. Mix, and after air jet milling, add 10-25% water. Then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0138] Preparation Example 10: 45% of Formula (I) compound tebuconazole water-dispersible granules (35:10)

[0139] Formulation: 35% of compound (I), 10% of tebuconazole, 3% sodium dodecyl sulfate, 8% of naphthalene sulfonate formaldehyde condensate, 1% of sodium fatty alcohol polyoxyethylene ether sulfate, 2% of sodium polycarboxylate, 5.5% sodium lignosulfonate, 5% attapulgite, and the balance is made up with kaolin.

[0140] Preparation method: Same as in preparation example 9.

[0141] Field efficacy trials

[0142] Field efficacy trial of wheat scab

[0143] Experimental basis: Refer to the People's Republic of China industry standard "Field Efficacy Test Guidelines for Pesticides Part 15: Fungicides for the Control of Wheat Fusarium Head (NY / T 1464.15-2007)";

[0144] Experiment location: Wheat field in Weifang City, Shandong Province;

[0145] Experimental target: Wheat scab;

[0146] Experimental crop and variety: Wheat (Zhengmai 9023);

[0147] Experimental setup: Each cell was randomly distributed across multiple blocks, with a guard row surrounding each cell. Each treatment was replicated four times, with each cell measuring 20m. 2 The cells are randomly arranged.

[0148] Application timing: First application at the early flowering stage (April 20th); second application 7 days later, for a total of 2 applications, using 450 L / hm² of water. 2 .

[0149] Test reagents:

[0150] Table 6 Experimental Treatments

[0151]

[0152]

[0153] Application method: Use conventional spraying method to spray evenly on both sides of the leaves.

[0154] Survey and statistical methods: The survey was conducted 10 days after the last application of the pesticide. Five points were randomly selected from the diagonal of each plot for sampling, with 20 plants surveyed at each point. The top 3 leaves of each plant were surveyed (including the flag leaf if present). The plants were graded according to the percentage of disease spots on each leaf covering the entire leaf area.

[0155] Grading method:

[0156] Level 0: No disease;

[0157] Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area;

[0158] Grade 3: The area of ​​lesions accounts for 6% to 25% of the total leaf area;

[0159] Level 5: The area of ​​lesions accounts for 26% to 50% of the total leaf area;

[0160] Level 7: The area of ​​lesions accounts for 51% to 75% of the total leaf area;

[0161] Level 9: The area of ​​diseased spots accounts for more than 76% of the total leaf area.

[0162] Methods for calculating drug efficacy

[0163] Calculate the disease index and prevention efficacy using the following formula.

[0164]

[0165] During the experiment, wheat growth was observed to be good in all treatment plots, and no herbicide damage was observed in any treatment.

[0166] The test results are shown in the table below:

[0167] Table 7. Results of field efficacy trials of various treatments for controlling wheat scab.

[0168]

[0169]

[0170] As shown in Table 7 above, the field efficacy trials revealed that the compound (I) and its mixtures with propiconazole, chlorfluazuron, tebuconazole, flutriafol, and tebuconazole exhibited good control effects against wheat scab. Ten days after the last application, the control efficacies of 26% compound (I)·propiconazole emulsifiable concentrate (16:10), 24% compound (I)·chlorfluazuron emulsifiable concentrate (4:20), 30% compound (I)·tebuconazole emulsifiable concentrate (10:20), 30% compound (I)·flutriafol suspension concentrate (18:12), and 18% compound (I)·tebuconazole suspension concentrate (12:6) against wheat scab were 83.16%, 82.27%, 82.64%, 82.62%, and 82.10%, respectively, all demonstrating good control effects.

[0171] No phytotoxicity or impact on crop growth and development was recorded during the experiment, indicating that the tested pesticide was safe for crops within the recommended dosage range. No effects on non-target organisms were found within the dosage range. Furthermore, no extreme weather events such as heavy rainfall or strong winds were recorded during the experiment.

[0172] Through indoor toxicity testing and field trials, the compound shown in formula (I) of this invention, combined with propiconazole, chlorfenapyr, tebuconazole, flutriafol, and tebuconazole, exhibited excellent control efficacy against wheat scab. The fungicidal composition or formulation obtained by this invention demonstrates significant efficacy, exhibiting high efficiency, broad spectrum, low residue, long-lasting effect, and strong systemic properties. Furthermore, no phytotoxicity was observed in the experiments, indicating that the enhanced synergistic effect of the obtained fungicidal composition or formulation reduces production and usage costs while ensuring crop safety.

[0173] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it based on the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A bactericidal composition containing triazole compounds, characterized in that: It comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I), with the following chemical structural formula: (I) Active ingredient B is a triazole compound, wherein the triazole compound is tebuconazole, and the mass ratio of the compound of formula (I) to tebuconazole is 1:24~22:

1.

2. The bactericidal composition according to claim 1, characterized in that, The mass ratio of the compound of formula (I) to tebuconazole is 1:24 to 16:

1.

3. The bactericidal composition according to claim 2, characterized in that, The mass ratio of the compound of formula (I) to tebuconazole is 1:24, 1:8, 1:2, 2:1, 8:1, or 16:

1.

4. The bactericidal composition according to claim 1, characterized in that, Based on a total weight of 100 wt% of the bactericidal composition, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 to 90 wt%.

5. The bactericidal composition according to claim 4, characterized in that, The sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 5-80 wt%.

6. The bactericidal composition according to claim 1, characterized in that, In addition to the active ingredient, the bactericidal composition contains agriculturally permissible auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, or carriers.

7. The bactericidal composition according to claim 6, characterized in that, The formulation of the bactericidal composition is selected from microemulsions, emulsifiable concentrates, suspensions, water-in-oil emulsions, water-dispersible granules, and seed-treated suspensions.

8. The application of the bactericidal composition according to any one of claims 1-7 in the prevention and control of plant diseases, wherein the plant disease is wheat scab caused by fungi.

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