A pesticide composition and use thereof

Abstract

The application discloses a kind of pesticide composition and its application, the pesticide composition includes active ingredient A and active ingredient B, the active ingredient A is Bifemetstrobin, the active ingredient B is any one of carbendazim, thiophanate-methyl, isoprothiolane, the mass ratio of the active ingredient A and active ingredient B is 1:60-42:1.The pesticide composition or its preparation of the application can enhance efficacy, reduce dosage, show good control effect on a variety of plant pathogens, have synergistic effect under certain mass ratio, and can delay the development of drug resistance, and is safe to environment.

Description

Technical Field

[0001] This invention belongs to the field of pesticide compound technology, specifically relating to a pesticide composition and its application. Background Technology

[0002] Rice is an important food crop, and rice blast is a highly destructive disease. Improper control can severely impact rice yield and seriously threaten my country's food security. The pathogen of rice blast is *Magnaporthe grisea*, belonging to the genus *Magnaporthe* in the phylum Ascomycota. Its asexual stage is *Pyricularia oryzae*, belonging to the genus *Pyricularia* in the deuteromycetes. Depending on the stage and location of infection, rice blast can be classified into seedling blast, leaf blast, leaf pillow blast, node blast, neck blast, and grain blast. Among these, neck blast is the most damaging, potentially leading to complete crop failure.

[0003] Bifemetstrobin is a methoxyacrylate bactericide developed by Sumitomo Chemicals, Inc. of Japan; CAS No.: 2454319-63-0, chemical structure as follows:

[0004]

[0005] Currently, the main pesticides used to control rice blast are triazoles, benzimidazoles, and disulfide heterocyclic compounds. However, due to long-term and irregular use of these pesticides, pathogens have developed resistance, posing significant challenges to disease control. Developing novel, highly efficient compound formulations by combining Bifemetstrobin with fungicides of different mechanisms of action is of great significance for overcoming and delaying pathogen resistance, reducing pesticide dosage, effectively controlling rice blast damage, and ensuring stable and high rice yields. Furthermore, no reports have been found regarding combinations of Bifemetstrobin with carbendazim, thiophanate-methyl, and isoprothiolane. Summary of the Invention

[0006] Based on the above, the purpose of this invention is to provide a pesticide composition and its application. This pesticide composition or its formulation can enhance efficacy, reduce dosage, and exhibit good control effects against a variety of plant pathogens. Under certain mass ratios, it has a synergistic effect, while also delaying the development of pesticide resistance and being environmentally safe.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: a pesticide composition comprising active ingredient A and active ingredient B, wherein active ingredient A is Bifemetstrobin, and active ingredient B is any one of carbendazim, thiophanate-methyl, and isoprothiolane.

[0008] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:60 to 42:1;

[0009] Furthermore, the mass ratio of active ingredient A to active ingredient B is 1:60 to 30:1, or any value within the above range.

[0010] Furthermore, active ingredient B is carbendazim, and the mass ratio of Bifemetstrobin to carbendazim is 1:60 to 22:1;

[0011] Furthermore, the mass ratio of Bifemetstrobin to carbendazim is 1:60, 1:46, 1:24, 1:12, 1:6, 1:3, 9:1, 22:1, or 42:1;

[0012] Furthermore, the mass ratio of Bifemetstrobin to carbendazim is 1:46 to 22:1;

[0013] Furthermore, the mass ratio of Bifemetstrobin to carbendazim is 1:46, 1:24, 1:12, 1:6, 1:3, 9:1, or 22:1.

[0014] Furthermore, active ingredient B is thiophanate-methyl, and the mass ratio of Bifemetstrobin to thiophanate-methyl is 1:42 to 28:1;

[0015] Furthermore, the mass ratio of Bifemetstrobin to thiophanate-methyl is 1:42, 1:28, 1:20, 1:15, 1:10, 10:1, 15:1, or 28:1.

[0016] Furthermore, the mass ratio of Bifemetstrobin to thiophanate-methyl is 1:28 to 28:1;

[0017] Furthermore, the mass ratio of Bifemetstrobin to thiophanate-methyl is 1:28, 1:20, 1:15, 1:10, 10:1, 15:1, or 28:1.

[0018] Furthermore, active ingredient B is isoprothiolane, and the mass ratio of Bifemetstrobin to isoprothiolane is 1:42 to 30:1;

[0019] Furthermore, the mass ratio of Bifemetstrobin to rice blast fungicide is 1:42, 1:30, 1:21, 2:25, 1:7, 8:1, 20:1, or 30:1.

[0020] Furthermore, the mass ratio of Bifemetstrobin to rice blast fungicide is 1:42 to 20:1;

[0021] Furthermore, the mass ratio of Bifemetstrobin to rice blast fungicide is 1:42, 1:30, 1:21, 2:25, 1:7, 8:1, or 20:1.

[0022] Furthermore, based on a total weight of 100 wt% for the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 1 to 80 wt%, or any value within the above range.

[0023] Furthermore, based on a total weight of 100 wt% for the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 5 to 70 wt%, or any value within the above range.

[0024] Furthermore, the pesticide composition includes agriculturally acceptable auxiliary ingredients in addition to the active ingredient;

[0025] Furthermore, the auxiliary components include one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, stabilizers, film-forming agents, warning colors, penetrants, and carriers;

[0026] Furthermore, the wetting agent is selected from one or more of sodium dodecylbenzene sulfate, sodium dodecylbenzene sulfonate, soapberry powder, alkyl sulfate, pull-apart powder BX, silkworm excrement, alkylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether, alkanolamide polyoxyethylene ether and its phosphate or sulfate salts, and alkyl polyoxyethylene ether succinate sulfonate.

[0027] Further, the dispersant is selected from one or more of polycarboxylate, lignin sulfonate, naphthalene or alkylnaphthalene formaldehyde condensate sulfonate, calcium alkylbenzene sulfonate, alkylphenol polyoxyethylene phosphate, fatty alcohol polyoxyethylene polyoxypropylene ether, fatty alcohol polyoxypropylene polyoxypropylene ether, and polymeric alkyl aryl sulfonate.

[0028] Furthermore, the emulsifier is selected from one or more of the following: fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrene-phenol polyoxyethylene ether, and fatty acid polyoxyethylene ester.

[0029] Furthermore, the thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organobentonite, magnesium aluminum silicate, and carboxymethyl cellulose;

[0030] Furthermore, the disintegrant is selected from one or more of aluminum chloride, bentonite, sucrose, modified starch, cellulose, urea, sodium carbonate, sodium bicarbonate, sodium chloride, sodium sulfate, citric acid, and tartaric acid;

[0031] Furthermore, the antifreeze is selected from one or more of alcohols, alcohol ethers, and inorganic salts, and is a mixture thereof;

[0032] Furthermore, the defoamer is selected from silicone oil, C 10 ~C 20 Saturated fatty acid compounds, C8-C 10 One or more of fatty alcohols or silicone compounds;

[0033] Further, the solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives, and deionized water;

[0034] Furthermore, the preservative is selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate;

[0035] Furthermore, the stabilizer is selected from one or more of oxalic acid, succinic acid, adipic acid, borax, and epoxidized vegetable oil;

[0036] Furthermore, the warning color is selected from any one or more of the following adjustment colors: blue, green, red, purple, and yellow;

[0037] Furthermore, the film-forming agent is selected from one or more of sodium carboxymethyl starch, sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol, and polyacrylic acid;

[0038] Furthermore, the carrier is selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and precipitated silica;

[0039] Furthermore, the pesticide composition can be prepared into any formulation permitted in agriculture, wherein the formulation is a solid formulation, a liquid formulation, or a seed treatment formulation;

[0040] Furthermore, the solid formulation is a water-dispersible granule or a wettable powder, and the liquid formulation is a water emulsion, a suspension, or an emulsifiable concentrate;

[0041] The present invention also discloses the application of the pesticide composition described above in the control of pathogenic fungi in agricultural, forestry, and horticultural plants.

[0042] Furthermore, the plant pathogen is *Pyricularia oryae*, the causal agent of rice blast.

[0043] Furthermore, the pesticide composition is applied in an effective amount to plant pathogens and / or their environment.

[0044] The present invention has the following advantages over the prior art:

[0045] 1) The pesticide composition or its formulation of the present invention is highly effective and broad-spectrum, effectively prevents diseases, is applicable to a wide range of crops, reduces the amount of pesticide used, and lowers production costs;

[0046] 2) The pesticide composition or its formulation of the present invention exhibits good control effects against a variety of plant pathogens and has a synergistic effect under certain mass ratios;

[0047] 3) The pesticide composition or its formulation of the present invention can delay the development of drug resistance in pathogenic bacteria. Detailed Implementation

[0048] To better illustrate the effective control effect of the present invention, the agents described in the above embodiments are used. To make the technical solution, objectives, and advantages of the present invention clearer, the present invention is illustrated with the following specific embodiments, but the present invention is not limited to these examples. The technical effect tests of the present invention employ a combination of indoor bioassays and field trials.

[0049] Preparation Example:

[0050] Preparation Example 1: 48% Bifemetstrobin·Carbendazim Wettable Powder (12:36)

[0051] Preparation Example 2: 24% Bifemetstrobin·Carbendazim Suspension (4:20)

[0052] Preparation Example 3: 44% Bifemetstrobin·Thiophanate-methyl wettable powder (4:40)

[0053] Preparation Example 4: 20% Bifemetstrobin·Thiophanate-methyl suspension (3:17)

[0054] Preparation Example 5: 8% Bifemetstrobin·Isoblast-M-O-Emulsifiable Concentrate (1:7)

[0055] Preparation Example 6: 12% Bifemetstrobin·Isobarbital EC (2:10)

[0056] Preparation Example 7: 46% Bifemetstrobin·Isoblastopyram wettable powder (6:40)

[0057] Specific formulation composition and preparation method:

[0058] 48% Bifemetstrobin·Carbendazim Wettable Powder (12:36)

[0059] Formula composition: 12% Bifemetstrobin, 36% Carbendazim, 1.5% Naphthalene sulfonate, 1.5% Sodium lignosulfonate, 4% Alkyl naphthalene sulfonate, 2.5% BX (a type of styrene), 5% Bentonite, 5% Silica, and Kaolin to make up the balance.

[0060] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0061] 24% Bifemetstrobin·Carbendazim Suspension (4:20)

[0062] Formula composition: 4% Bifemetstrobin, 20% Carbendazim, 1.5% Gelbert alcohol polyoxyethylene ether, 4% Styrene phenol polyoxyethylene ether phosphate, 2% Ethylene glycol oxyethylene polyoxypropylene ether, 1% Naphthalene sulfonate formaldehyde condensate, 0.25% Xanthan gum, 1% Magnesium aluminum silicate, 5% Glycerol, 0.1% Potassium benzoate, 0.5% Organosilicon defoamer, deionized water to make up the balance.

[0063] Preparation method: The active ingredients, surfactants and other functional additives are placed in a reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenized filtration to obtain the suspension product.

[0064] 44% Bifemetstrobin·Thiophanate-methyl wettable powder (4:40)

[0065] Formula composition: 4% Bifemetstrobin, 40% Thiophanate-methyl, 1.5% Sodium lignosulfonate, 4% Alkyl naphthalene sulfonate, 2.5% BX (a type of styrene-based powder), 4% Naphthalene sulfonate formaldehyde condensate, 10% fumed silica, 3% starch, and kaolin to make up the balance.

[0066] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder product of the composition of the present invention.

[0067] 20% Bifemetstrobin·Thiophanate-methyl suspension (3:17)

[0068] Formula composition: 3% Bifemetstrobin, 17% methyl thiophanate, 1.5% Gelbert alcohol polyoxyethylene ether, 4% styrene phenol polyoxyethylene ether phosphate, 2% ethylene glycol oxyethylene polyoxypropylene ether, 1% sodium polycarboxylate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 5% glycerol, 0.1% potassium benzoate, 0.5% silicone defoamer, deionized water to make up the balance;

[0069] Preparation method: The active ingredients, surfactants and other functional additives are placed in a reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenized filtration to obtain the suspension product.

[0070] 8% Bifemetstrobin·Isoblast-K (1:7) Emulsifiable Concentrate

[0071] Formula composition: 1% Bifemetstrobin, 7% Isoprothiolane, 4% Xylene, 10% Cyclohexanone, 1.2% BHT, 2% Castor oil polyoxyethylene ether phosphate, 5% Ethylene oxide-propylene oxide copolymer, 5% Glycerin, 0.1% Silicone oil, 0.1% Xanthan gum, 0.1% Sodium benzoate, deionized water to make up the balance;

[0072] Preparation method: The surfactant, antifreeze and water are mixed to form an aqueous phase. Then, the active ingredient is dissolved in a solvent and added to the aqueous phase under stirring. After stirring evenly, the mixture is sheared to a particle size D. 90 ≤5μm, add thickener and preservative and continue shearing for 10 minutes, then add defoamer and stir evenly to form an O / W type water emulsion product.

[0073] 12% Bifemetstrobin (2:10) Emulsifiable Oil

[0074] Formulation composition: 2% Bifemetstrobin, 10% Isoprothiolane, 15% EO / PO block copolymer, 2% castor oil polyoxyethylene ether, 15% acetophenone, 12% propylene glycol methyl ether, 2% calcium dodecylbenzenesulfonate, xylene to make up the balance;

[0075] Preparation method: The metered active ingredients, solvent, and co-solvent are added to the mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the emulsifiable oil product required by this invention.

[0076] 46% Bifemetstrobin·Isoblast-resistant wettable powder (6:40)

[0077] Formula composition: 6% Bifemetstrobin, 40% Isoprothiolane, 1% Naphthalenesulfonate, 1.5% Sodium lignosulfonate, 4% Alkyl naphthalenesulfonate, 2.5% BX (a type of pyrrolidone), 4% Naphthalenesulfonate formaldehyde condensate, 5% Kaolin, 8% Silica, with Kaolin to make up the balance;

[0078] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder product of the composition of the present invention.

[0079] Indoor activity test

[0080] Example 1: Indoor combined effect test of rice blast disease

[0081] Test basis:

[0082] "Guidelines for Indoor Bioassay Testing of Pesticides" - Fungicides Part 2: Plate Test for Inhibition of Mycelial Growth of Pathogenic Fungi NY / T 1156.2-2006;

[0083] "Guidelines for Indoor Bioassay Testing of Pesticides" - Fungicides Part 6: Determination of Combined Effects of Mixtures NY / T1156.6-2006;

[0084] Experimental target: Rice blast fungus (Pyricularia oryzae Cav.).

[0085] Test agents: 95% Bifemetstrobin technical grade, 92% carbendazim technical grade, 97% thiophanate-methyl technical grade, and 95% isoprothiolane technical grade; all of the above technical grades were provided by the Group's R&D Center.

[0086] Reagent preparation: Dissolve the above test reagents in a suitable solvent, then dilute with 0.1% Tween-80 aqueous solution, and set the required series of mass concentrations according to the reagent activity.

[0087] Experimental Method: Under aseptic conditions, the drug was added to the melted PDA medium at a specific ratio, mixed, and poured into sterilized petri dishes to prepare a series of drug-containing culture plates with varying concentrations. The tested rice blast fungus strain was first incubated on PDA medium at 25°C for 7–10 days. When the colonies reached the edge of the petri dish, mycelial cakes were collected from the edge of the colonies using a sterilized punch, and then transferred to the center of the aforementioned drug-containing culture plates. A plate without the drug served as a control.

[0088] Experimental treatment: One mycelium cake was inoculated on each plate, and each treatment was repeated 4 times.

[0089] Investigation Methods: After inoculation, the culture dishes were placed in a biochemical incubator and cultured at (25±1)℃. The mycelial growth of the pathogen was investigated based on the mycelial growth in the blank control culture dishes. The diameter of each colony was measured with calipers in millimeters (mm). The diameter of each colony was measured vertically once using the cross-sectional method, and the average value was taken.

[0090] Data statistics and calculation methods: Based on the survey results, the inhibition rate of mycelial growth of the tested target bacteria at each treatment concentration was calculated using the following formula. Based on the logarithmic values ​​of each agent concentration and the corresponding inhibition probability values, a statistical analysis system was used to analyze the data and derive the virulence regression equation and EC50. 50 The values ​​and correlation coefficients are used to evaluate the activity of the test reagent on the biological sample.

[0091]

[0092] In the formula:

[0093] I—colony growth inhibition rate;

[0094] D0—Correlation diameter of the blank control;

[0095] D t —The diameter of colony growth after chemical treatment.

[0096] The co-toxicity coefficient (CTC) of the mixture was calculated using Sun Yunpei's method to evaluate the type of combined effects.

[0097] CTC ≥ 120 exhibits a synergistic effect, CTC ≤ 80 exhibits an antagonistic effect, and CTC between 80 and 120 exhibits an additive effect.

[0098] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0099]

[0100] In the formula:

[0101] ATI – Actual Measured Toxicity Index of Mixtures;

[0102] S – EC of standard bactericides 50 The unit is milligrams per liter (mg / L);

[0103] M – EC of the mixture 50 The unit is milligrams per liter (mg / L).

[0104] TTI = TI A *P A +TI B *P B

[0105] In the formula:

[0106] TTI – Theoretical Toxicity Index of Mixtures;

[0107] TI A —A. Toxicity index of drug A;

[0108] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0109] TI B —Toxicity index of drug B;

[0110] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0111]

[0112] In the formula:

[0113] CTC – Cotoxicity Coefficient;

[0114] ATI – Actual Measured Toxicity Index of Mixtures;

[0115] TTI – Theoretical Toxicity Index of Mixtures.

[0116] Indoor test results:

[0117] The results in Table 1 show that all tested agents exhibited good inhibitory effects against rice blast fungus. Specifically, when the mass ratio of Bifemetstrobin to carbendazim was between 1:60 and 42:1, the co-toxicity coefficient was greater than 80, indicating an additive or synergistic effect. When the mass ratio of Bifemetstrobin to carbendazim was between 1:60 and 22:1, the co-toxicity coefficient was greater than 120, demonstrating a significant synergistic effect. The highest co-toxicity coefficient, 213.588, was observed when the mass ratio of Bifemetstrobin to carbendazim was 1:3.

[0118] Table 1. Results of the indoor combined effect test of Bifemetstrobin and carbendazim on rice blast disease.

[0119]

[0120] As shown in Table 2, when the mass ratio of Bifemetstrobin to thiophanate-methyl is between 1:42 and 28:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect. When the mass ratio of Bifemetstrobin to thiophanate-methyl is between 1:28 and 28:1, the co-toxicity coefficient is greater than 130, indicating a significant synergistic effect. When the mass ratio of Bifemetstrobin to thiophanate-methyl is 1:10, the co-toxicity coefficient is the highest, at 224.306.

[0121] Table 2. Results of indoor combined treatment of Bifemetstrobin and thiophanate-methyl against rice blast.

[0122]

[0123]

[0124] As shown in Table 3, when the mass ratio of Bifemetstrobin to pyrethroid was between 1:42 and 30:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect. When the mass ratio of Bifemetstrobin to pyrethroid was between 1:42 and 20:1, the co-toxicity coefficient was greater than 140, indicating a significant synergistic effect. When the mass ratio of Bifemetstrobin to pyrethroid was 1:7, the co-toxicity coefficient was the highest at 275.181.

[0125] Table 3. Results of indoor combined effects of Bifemetstrobin and isoprothiolane on rice blast disease.

[0126]

[0127] Example 2: Field efficacy trial of rice blast disease

[0128] Experimental basis: The experiment was conducted in accordance with GB / T 17980.19-2000 "Field Efficacy Test Guidelines (I) Fungicides for the Control of Leaf Diseases in Rice".

[0129] Experimental site: The experiment was conducted in the rice experimental field of Tianli Village, Daowai District, Harbin City, Heilongjiang Province. The experimental site is flat, fertile, and the soil is albic soil. The crop varieties, planting time, growth, fertilizer and water management conditions of each experimental plot are basically the same.

[0130] Test reagents and dosages:

[0131] Table 4 Test reagents and dosages

[0132]

[0133]

[0134] Experimental Design: The experiment consisted of 8 treatments, each replicated 4 times, with water as a blank control. The plot area was 60m². 2 Each community is randomly arranged into blocks, and adjacent communities are protected by a row.

[0135] Application time and frequency: The experiment was conducted by applying the pesticide once at the early stage of rice heading (heading rate 5% to 10%), using 30L of water per mu, and using a 3WBD-16L electric sprayer for uniform spraying.

[0136] Experimental field conditions: No other fungicides were used in any area before and during the experiment, and the management level was consistent in all areas.

[0137] Survey method: Five sampling points were used in each plot, with 50 panicles at each point, for a total of 250 panicles in each plot. The survey was conducted once. Before the rice matured and was harvested, the number of diseased panicles at each level in each plot was investigated, and the disease index and control effect were calculated.

[0138] Safety investigation of pesticides: During the experiment, the rice grew normally, and none of the pesticides at the tested concentrations caused phytotoxicity to the rice plants or other non-target organisms.

[0139] Record the classification according to the following classification method:

[0140] Level 0: No disease;

[0141] Grade 1: Less than 5% loss per ear (individual branches affected);

[0142] Level 3: 6%–20% loss per ear (about one-third of the branches and stalks are affected);

[0143] Grade 5: 21%–50% loss per ear (disease on the neck or main axis, grains half-empty);

[0144] Level 7: 51%–70% loss per ear (neck disease, most ears are empty);

[0145] Grade 9: 71% to 100% loss per ear (caused by disease at the neck of the ear, resulting in white ears).

[0146] The efficacy of the drug is calculated using the following formula:

[0147]

[0148] The results of the field efficacy trials are shown in the table below:

[0149] The analysis of the field efficacy test results in Table 5 shows that Bifemetstrobin, when combined with carbendazim, thiophanate-methyl, and isoprothiolane, exhibits good control efficacy against rice blast. A survey of rice panicle blast conducted before harvest after application of the pesticides showed that the control efficacies of 24% Bifemetstrobin·carbendazim suspension (4:20), 20% Bifemetstrobin·thiophanate-methyl suspension (3:17), and 12% Bifemetstrobin·isoprothiolane emulsifiable concentrate (2:10) were 87.05%, 86.59%, and 88.02%, respectively.

[0150] Table 5. Field efficacy test results of each tested agent against rice blast.

[0151]

[0152] In summary, through indoor toxicity testing and field efficacy trials, it can be seen that the pesticide composition of Bifemetstrobin of the present invention, combined with carbendazim, thiophanate-methyl, and isoprothiolane, has a good control effect on rice blast disease, is safe for rice, and has significant control efficacy. It is superior to single agents in delaying the development of resistance and prolonging the duration of efficacy, and can effectively reduce costs and pesticide residues.

[0153] Although this application describes specific embodiments in detail with the aid of examples, the disclosure of this application can be modified and substituted in various ways. However, it should be understood that the disclosure of this application is not limited to the specific form disclosed. Rather, the disclosure of this application covers all modifications, equivalents, and substitutions within the scope of the disclosure of this application, the scope of which is defined by the appended claims and their legal equivalents.

Claims

1. A pesticide composition, characterized in that, The pesticide composition comprises active ingredient A and active ingredient B, wherein active ingredient A is Bifemetstrobin and active ingredient B is isoprothiolane, and the mass ratio of Bifemetstrobin to isoprothiolane is 1:42 to 30:

1.

2. The pesticide composition according to claim 1, characterized in that, The mass ratio of Bifemetstrobin to rice blast fungicide is 1:42 to 20:

1.

3. The pesticide composition according to claim 1, characterized in that, The mass ratio of Bifemetstrobin to rice blast fungicide is 1:42, 1:30, 1:21, 2:25, 1:7, 8:1, 20:1, and 30:

1.

4. The pesticide composition according to claim 1, characterized in that, The total weight of the pesticide composition is 100 wt%, and the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 1 to 80 wt%.

5. The pesticide composition according to claim 4, characterized in that, The sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 5-70 wt%.

6. The pesticide composition according to claim 5, characterized in that, In addition to the active ingredient, the pesticide composition also includes auxiliary components, which include one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, stabilizers, film-forming agents, warning colors, penetrants, and carriers.

7. The pesticide composition according to claim 1, characterized in that, The pesticide composition is prepared into formulations such as water-dispersible granules, wettable powders, water-in-oil emulsions, suspensions, and emulsifiable concentrates.

8. The application of the pesticide composition according to any one of claims 1-7 in controlling pathogenic fungi in agricultural, forestry, or horticultural plants, characterized in that, The plant pathogen mentioned is rice blast fungus.

9. The application as described in claim 8, characterized in that, The pesticide composition is applied to plant pathogens and / or their environment in an effective amount.

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