Silk fibroin gel dressing as well as preparation method and application thereof

Abstract

The invention relates to a silk fibroin gel dressing and a preparation method and application thereof, the silk fibroin gel dressing comprises a silk fibroin gel dressing main body formula, and the silk fibroin gel dressing main body formula comprises water, silk fibroin, an inducer, a protective agent and a thickening agent; wherein the mass fraction of the silk fibroin is 0.1%-5%, the mass fraction of the inducer is 1%-40%, the mass fraction of the protective agent is 1%-10%, the mass fraction of the thickening agent is 0.1%-2%, and the balance is water. The prepared silk fibroin gel dressing is easy to smear and absorb and does not need to be cleaned; compared with traditional dressings, safer components are adopted, and the dressing is mild, painless and non-irritant; through the synergistic effect of the various components, multi-dimensional protection can be formed on the skin surface, the skin barrier injury can be repaired, the effects of moisturizing, relieving, red fading and stability maintaining are achieved, postoperative skin discomfort can be relieved, and wound healing can be promoted.

Description

Technical Field

[0001] The present invention relates to the technical field of the application of silk fibroin, and particularly relates to a silk fibroin gel dressing, a preparation method thereof and an application thereof. Background Art

[0002] Traditional wound dressings have long relied on passive protection mechanisms of physical isolation and absorption of exudate, and have limitations such as delayed healing caused by environmental dryness and secondary damage caused by adhesion between the material and the wound surface, especially with poor effects in chronic wound management. In recent years, hydrogel dressings based on the theory of moist wound healing have become a research hotspot in the field of wound care due to their high water content and biomimetic structural characteristics. Further combining silk fibroin, a natural biomaterial, can significantly optimize the biological function and wound adaptability of the hydrogel.

[0003] Silk fibroin is derived from natural silk, and its unique β-sheet crystal structure and hydrophilic amino acid composition (such as glycine and alanine) endow the hydrogel dressing with dual advantages: 1) dynamic regulation of the moist environment; 2) upgrade of biological activity and tissue compatibility. By combining silk fibroin with hydrogel technology, the new generation of dressings not only overcomes the dryness and adhesion defects of traditional dressings, but also provides an innovative solution with both biomimetic structure and functional initiative for complex wound healing through the integration of natural active ingredients and precise regulation of the microenvironment.

[0004] Chinese Patent (CN201710088689.1) discloses an invisible and peelable silk fibroin nursing film substrate and a preparation method thereof. The substrate mainly consists of silk fibroin, and is equipped with a moisturizer and a thickener. The raw materials of this substrate are all natural, and the preparation method and steps are simple. A layer of invisible film with aesthetic effects can be formed on the skin in a short time. The film material is similar to the skin, is easy to peel off, and has good compatibility with active substances. However, this substrate has great limitations when used for wounds. When used for slightly larger wounds, it cannot form a complete film, and it needs to be torn multiple times to be completely peeled off. Moreover, when the wound is pulled, the formed film is prone to local bulging, which is not beautiful and cannot play a good role in covering the wound surface. When using, the coating amount of the substrate is not easy to control. If it is thinly coated, it is not easy to form a complete film and is easy to rub into mud. If it is thickly coated, the substrate accumulates on the skin surface, does not form a film and cannot be absorbed, and is difficult to clean. Secondary injury to the wound is easily caused during cleaning.

[0005] Chinese Patent (CN201910984317.6) discloses a skin repair dressing and a preparation method thereof. The dressing mainly consists of silk fibroin and / or silk peptide, hyaluronate, glycerol, preservative and water, and can form a protective barrier on the skin surface to promote the healing of skin surface wounds. However, the addition of preservative components in this dressing may cause certain irritation to the damaged skin or wound surface.

[0006] Chinese Invention Patent (CN202210758330.1) discloses a silk fibroin hydrogel dressing and its preparation method and application. This dressing is composed of 0.01% - 15% silk fibroin and 85% - 99.9% water. The composition of this dressing is single, and the effect of repairing skin damage is limited. Summary of the Invention

[0007] The purpose of the present invention is to address the deficiencies in the prior art and provide a silk fibroin gel dressing and its preparation method and application. This silk fibroin gel dressing is easy to apply and absorb, and does not require washing; compared with traditional dressings, it uses safer ingredients, is mild, painless, and non-irritating; multiple ingredients act synergistically to form a multi-dimensional protection on the skin surface, repair skin barrier damage, achieve the effects of moisturizing, soothing, reducing redness, and stabilizing, relieve postoperative skin discomfort, and promote wound healing. To achieve the above purpose, the technical solutions adopted by the present invention are as follows:

[0008] In the first aspect, a silk fibroin gel dressing includes a main formula of the silk fibroin gel dressing, and the main formula of the silk fibroin gel dressing includes water, silk fibroin, an inducer, a protective agent, and a thickening agent;

[0009] Among them, the mass fraction of the silk fibroin is 0.1% - 5%, the mass fraction of the inducer is 1% - 40%, the mass fraction of the protective agent is 1 - 10%, the mass fraction of the thickening agent is 0.1% - 2%, and the balance is water.

[0010] In some embodiments, the inducer includes one or more of polyethylene glycol 300, polyethylene glycol 400, polyethylene glycol 600, polyethylene glycol 1000, glycerol, butanediol, propylene glycol, pentanediol, and hexanediol.

[0011] In some embodiments, the protective agent includes one or more of trehalose, glycerol, and poloxamer.

[0012] In some embodiments, the thickening agent includes one or more of carbomer, carrageenan, sodium alginate, xanthan gum, and hydroxyethyl cellulose.

[0013] In some embodiments, when the thickening agent is carbomer, the silk fibroin-gel dressing formula further includes triethanolamine and / or sodium hydroxide.

[0014] In some embodiments, it further includes an auxiliary formula of the silk fibroin gel dressing, and the auxiliary formula of the silk fibroin gel dressing includes one or more of dextran, hyaluronic acid, collagen, and chitosan;

[0015] Among them, the mass fraction of dextran is 0.01%-10%, the mass fraction of hyaluronic acid is 0.1%-1.5%, the mass fraction of collagen is 0.01%-2%, and the mass fraction of chitosan is 0.1%-3%.

[0016] In a second aspect, the present invention also provides a method for preparing a silk fibroin gel dressing, comprising:

[0017] Adding a thickener with a mass fraction of 0.1% to 2% into water and stirring evenly to obtain a first mixed solution;

[0018] Adding an inducer with a mass fraction of 1% to 40% and a protective agent with a mass fraction of 1 to 10% into the first mixed solution in sequence and stirring evenly to obtain a second mixed solution;

[0019] Adding silk fibroin with a mass fraction of 0.1% to 5% into the second mixed solution and stirring evenly to obtain a third mixed solution;

[0020] Performing moist heat sterilization or irradiation sterilization on the third mixed solution to obtain the silk fibroin gel dressing.

[0021] In some of these embodiments, the stirring speed for adding the thickener with a mass fraction of 0.1% to 2% into water and stirring evenly to obtain the first mixed solution is 150 to 250 rpm; and / or

[0022] The stirring temperature for adding the inducer with a mass fraction of 1% to 40% and the protective agent with a mass fraction of 1 to 10% into the first mixed solution in sequence and stirring evenly to obtain the second mixed solution is 40°C to 50°C, and the stirring speed is 100 to 200 rpm; and / or

[0023] The stirring temperature for adding silk fibroin with a mass fraction of 0.1% to 5% into the second mixed solution and stirring evenly to obtain the third mixed solution is 50°C to 60°C, and the stirring speed is 250 to 350 rpm.

[0024] In some of these embodiments, adding the thickener with a mass fraction of 0.1% to 2% into water and stirring evenly to obtain the first mixed solution further includes:

[0025] After adding the thickener with a mass fraction of 0.1% to 2% into water, adding one or more of dextran, hyaluronic acid, collagen, and chitosan thereto and stirring evenly to obtain the first mixed solution.

[0026] In a third aspect, the present invention also provides the application of a silk fibroin gel dressing according to any one of claims 1 to 6 in the fields of medical aesthetics, medical devices, and cosmetics, which is characterized by including the care and repair of chronic and non-chronic wounds, as well as skin moisturization, soothing, and anti-aging.

[0027] The present invention adopts the above technical solutions and has the following technical effects compared with the prior art:

[0028] A fibroin gel dressing of the present invention, its preparation method and application are easy to apply and absorb, and do not need to be cleaned; compared with traditional dressings, safer ingredients are used, which are mild, painless and non-irritating; the synergistic effect of multiple ingredients can form multi-dimensional protection on the skin surface, repair skin barrier damage, achieve the effects of moisturizing, soothing, reducing redness and stabilizing, relieve postoperative skin discomfort, and promote wound healing of the wound surface. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 are the experimental data results of the cell proliferation experiment in the examples of the present invention;

[0030] Figure 2 are the experimental data results of the ROS (reactive oxygen species) level test in the examples of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] In order to make the objectives, technical solutions and advantages of the present application clearer, the present application will be described and explained below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application. Based on the embodiments provided in the present application, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of protection of the present application.

[0032] Obviously, the accompanying drawings in the following description are only some examples or embodiments of the present application. For those of ordinary skill in the art, without making creative efforts, the present application can also be applied to other similar scenarios based on these drawings. In addition, it can also be understood that although the efforts made in this development process may be complex and lengthy, for those of ordinary skill in the art related to the content disclosed in the present application, some design, manufacturing or production changes made on the basis of the technical content disclosed in the present application are only conventional technical means and should not be understood as the content disclosed in the present application being insufficient.

[0033] Reference to "embodiments" in the present application means that the specific features, structures or characteristics described in connection with the embodiments can be included in at least one embodiment of the present application. The phrase appears in various places in the specification and does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment mutually exclusive with other embodiments. Those of ordinary skill in the art explicitly and implicitly understand that the embodiments described in the present application can be combined with other embodiments without conflict.

[0034] Example 1

[0035] This embodiment relates to the fibroin gel dressing in the present invention.

[0036] In a schematic embodiment of the present invention, a fibroin gel dressing includes a main formulation of the fibroin gel dressing, and the main formulation of the fibroin gel dressing includes water, fibroin, an inducer, a protective agent, and a thickener; wherein, the mass fraction of the fibroin is 0.1% - 5%, the mass fraction of the inducer is 1% - 40%, the mass fraction of the protective agent is 1 - 10%, the mass fraction of the thickener is 0.1% - 2%, and the balance is water.

[0037] It should be noted that the fibroin gel dressing can be in a liquid, gel or paste form.

[0038] In some of these embodiments, the inducer includes one or more of polyethylene glycol 300, polyethylene glycol 400, polyethylene glycol 600, polyethylene glycol 1000, glycerol, butanediol, propylene glycol, pentylene glycol, hexylene glycol; preferably, the inducer includes polyethylene glycol 400.

[0039] In some of these embodiments, the protective agent includes one or more of trehalose, glycerol, poloxamer; preferably, the protective agent includes trehalose and glycerol.

[0040] In some of these embodiments, the thickener includes one or more of carbomer, carrageenan, sodium alginate, xanthan gum, hydroxyethyl cellulose; preferably, the thickener includes carbomer.

[0041] Further, when the thickener is carbomer, the main formulation of the fibroin gel dressing further includes triethanolamine and / or sodium hydroxide; preferably, the main formulation of the fibroin gel dressing further includes triethanolamine.

[0042] In this embodiment, the main formulation of the fibroin gel dressing includes 0.1% by mass of fibroin, 0.4% by mass of carbomer, 10% by mass of trehalose, 10% by mass of glycerol, 15% by mass of polyethylene glycol 400, 1% by mass of triethanolamine, and the balance is purified water.

[0043] The advantages of this embodiment are that it is easy to apply and absorb, and there is no need to wash; compared with traditional dressings, it uses safer ingredients, is gentle, painless and non - irritating; the synergistic effect of multiple ingredients can form a multi - dimensional protection on the skin surface, repair skin barrier damage, achieve the effects of moisturizing, soothing, reducing redness and maintaining stability, and can relieve postoperative skin discomfort and promote wound healing of the wound surface.

[0044] Example 2

[0045] This embodiment is a variant embodiment of Example 1.

[0046] The main formula of the fibroin gel dressing includes 0.1% by mass of fibroin, 0.8% by mass of carbomer, 5% by mass of trehalose, 10% by mass of glycerol, 15% by mass of polyethylene glycol 400, 1% by mass of triethanolamine, and the balance is purified water.

[0047] The advantages of this example are the same as those of Example 1 and will not be elaborated here.

[0048] Example 3

[0049] This example is a variant example of Examples 1 to 2.

[0050] The main formula of the fibroin gel dressing includes 3% by mass of fibroin, 0.4% by mass of carbomer, 10% by mass of trehalose, 10% by mass of glycerol, 15% by mass of polyethylene glycol 400, 1% by mass of triethanolamine, and the balance is purified water.

[0051] The advantages of this example are the same as those of Example 1 and will not be elaborated here.

[0052] Example 4

[0053] This example is a variant example of Examples 1 to 3.

[0054] The main formula of the fibroin gel dressing includes 3% by mass of fibroin, 0.8% by mass of carbomer, 5% by mass of trehalose, 10% by mass of glycerol, 15% by mass of polyethylene glycol 400, 1% by mass of triethanolamine, and the balance is purified water.

[0055] The advantages of this example are the same as those of Example 1 and will not be elaborated here.

[0056] Example 5

[0057] This example is a variant example of Examples 1 to 4.

[0058] The main formula of the fibroin gel dressing includes 5% by mass of fibroin, 0.4% by mass of carbomer, 10% by mass of trehalose, 10% by mass of glycerol, 15% by mass of polyethylene glycol 400, 1% by mass of triethanolamine, and the balance is purified water.

[0059] The advantages of this example are the same as those of Example 1 and will not be elaborated here.

[0060] Example 6

[0061] This embodiment is a variant embodiment of Embodiments 1 to 5.

[0062] The main formula of the silk fibroin gel dressing includes 5% by mass of silk fibroin, 0.8% by mass of carbomer, 5% by mass of trehalose, 10% by mass of glycerol, 15% by mass of polyethylene glycol 400, 1% by mass of triethanolamine, and the balance is purified water.

[0063] The advantages of this embodiment are the same as those of Embodiment 1 and will not be elaborated here.

[0064] Embodiment 7

[0065] This embodiment is a variant embodiment of Embodiments 1 to 6.

[0066] The silk fibroin gel dressing further includes an auxiliary formula for the silk fibroin gel dressing, and the auxiliary formula for the silk fibroin gel dressing includes one or more of dextran, hyaluronic acid, collagen, and chitosan; wherein, the mass fraction of dextran is 0.01% to 10%, the mass fraction of hyaluronic acid is 0.1% to 1.5%, the mass fraction of collagen is 0.01% to 2%, and the mass fraction of chitosan is 0.1% to 3%.

[0067] Preferably, the mass fraction of dextran is 0.1%, the mass fraction of hyaluronic acid is 0.1%, the mass fraction of collagen is 0.05%, and the mass fraction of chitosan is 1%.

[0068] The advantages of this embodiment are the same as those of Embodiment 1 and will not be elaborated here.

[0069] Embodiment 8

[0070] The physical and chemical properties of the silk fibroin gel dressings prepared in Embodiments 1 to 6 were tested, and the indicators included appearance, pH, and viscosity. The appearance of the silk fibroin gel dressing was observed visually, the pH index was detected using a pH meter, and the viscosity index was detected using a rotational viscometer. The test results are as follows:

[0071]

[0072] Carbomer and triethanolamine act synergistically to form a stable polymer structure with excellent adhesion properties. It can be seen from the comparison between Embodiment 3 and Embodiment 4 that the higher the carbomer content, the more viscous the texture of the gel dressing.

[0073] It can be seen from the comparison between Embodiment 5 and Embodiment 6 that after carbomer is compounded with silk fibroin, the viscosity is significantly increased.

[0074] Therefore, by adjusting the ratios of the three components, an ideal gel dressing product can be obtained. This silk fibroin gel dressing can form a multi-dimensional protection on the skin surface, ensuring effective isolation between the damaged skin wound and the external environment, thus achieving the purpose of protection.

[0075] There were no significant changes in the physical and chemical properties of each example in the above table before and after moist heat sterilization. It can be seen that the silk fibroin gel dressing prepared under this formulation process is relatively stable, and the silk fibroin has formed a stable β-sheet structure.

[0076] Example 9

[0077] Perform sensory evaluation on the silk fibroin gel dressings prepared in Examples 1 - 6. Apply them to the skin on the inner side of the wrist and observe the gel texture, spreadability, skin feel, and irritation.

[0078]

[0079]

[0080] As can be seen from Example 4 and Example 6, when the viscosity of the silk fibroin gel dressing is relatively high, it is not easy to spread during application, the skin feel is sticky, and the experience is not good.

[0081] As can be seen from Example 1, Example 2, Example 3, and Example 5, the silk fibroin gel dressing with appropriate viscosity has a uniform texture, smooth spreadability, is cool and moist after being applied to the skin, is mild and non-irritating, and can relieve postoperative skin discomfort.

[0082] Example 10

[0083] Perform skin water content testing on the silk fibroin gel dressings prepared in Examples 1 - 6. Apply them to the cheeks, massage and absorb after 10 minutes, and use a skin moisture tester to detect the skin moisture, elasticity, and oiliness of the cheeks before and after application. The whole test is carried out in an environment with constant temperature and humidity. The test results are as follows:

[0084]

[0085] As can be seen from the above table, the silk fibroin gel dressing provided by the present invention has a good effect of moisturizing the skin, can improve skin elasticity, and achieves the effects of moisturizing and soothing, and repairing skin barrier damage.

[0086] Example 11

[0087] Conduct tests according to the method specified in the liquid affinity of amorphous hydrogel dressings in 3.4 of Part 1: Liquid Absorbency of YY / T 0471.1 - 2004 "Test Methods for Contact Wound Dressings" to detect the ability of the silk fibroin gel dressing to absorb liquid from agar and deliver liquid to gelatin, so as to evaluate the liquid affinity of the gel dressing.

[0088]

[0089]

[0090] As can be seen from Example 1 and Example 2, when the content of silk fibroin is low, the ability of the silk fibroin gel dressing to absorb wound exudate is limited.

[0091] As can be seen from Example 6, when the content of silk fibroin is high, the ability to provide moisture to the skin is weakened.

[0092] As can be seen from Example 3 and Example 5, the addition of trehalose plays a synergistic role with silk fibroin. While absorbing wound exudate, it can also provide a certain amount of moisture to the skin, creating a moist healing environment in a physiological state for wound healing and promoting the healing of wound surfaces.

[0093] Example 12

[0094] Cell proliferation experiments were carried out on the silk fibroin gel dressings prepared in Examples 1 to 6. The specific method is as follows:

[0095] Fibroblasts were inoculated into 96-well plates for culture. The culture medium was (DMEM medium containing 10% FBS), and the culture environment was 37 °C, 5% CO 2 , saturated humidity. After culturing for 24 h, the silk fibroin gel dressing samples were placed in the culture solution. After incubating for 72 h, CCK 8 was added, and then the absorbance value at 450 nm was detected using a microplate reader.

[0096] As Figure 1 shown, after co-culturing with the silk fibroin gel for 72 h, the fibroblasts in the groups of Examples 1 to 6 grew significantly compared with the blank group, indicating that the silk fibroin gel dressing can promote the growth and proliferation of fibroblasts.

[0097] Example 13

[0098] ROS (reactive oxygen species) level tests were carried out on the silk fibroin gel dressings prepared in Examples 1, 3, and 5. The specific method is as follows:

[0099] Fibroblasts were inoculated into 12-well plates for culture. The culture medium was (DMEM medium containing 10% FBS), and the culture environment was 37 °C, 5% CO 2 , saturated humidity. After culturing for 48 h, the silk fibroin gel dressing samples were placed in the culture solution. After incubating for 48 h, DCFH-DA fluorescence was added, and then irradiated under UVA. Immediately after the irradiation ended, the fluorescence intensity was detected using a fluorescence microplate reader.

[0100] Experimental grouping: The negative group was fibroblasts without irradiation; the positive group was fibroblasts after irradiation; the control group was fibroblasts supplemented with 0.5 mg / mL vitamin C; the experimental group was the silk fibroin gel dressings of Example 1, Example 3, and Example 5 supplemented with fibroblasts.

[0101] As Figure 2 shown, compared with the positive group, the ROS (reactive oxygen species) levels in the experimental group decreased to varying degrees, indicating that the silk fibroin gel dressing can reduce the reactive oxygen species (ROS) level.

[0102] Example 14

[0103] This example relates to the preparation method of the silk fibroin gel dressing in the present invention.

[0104] A preparation method of a silk fibroin gel dressing, comprising:

[0105] Adding a thickener with a mass fraction of 0.1% - 2% to water and stirring evenly to obtain a first mixed solution;

[0106] Adding an inducer with a mass fraction of 1% - 40% and a protective agent with a mass fraction of 1 - 10% to the first mixed solution in sequence and stirring evenly to obtain a second mixed solution;

[0107] Adding silk fibroin with a mass fraction of 0.1% - 5% to the second mixed solution and stirring evenly to obtain a third mixed solution;

[0108] Performing moist heat sterilization on the third mixed solution to obtain the silk fibroin gel dressing.

[0109] In some of these embodiments, the thickener includes but is not limited to carbomer.

[0110] It should be noted that in the case where the thickener is carbomer, triethanolamine also needs to be added.

[0111] In some of these embodiments, the inducer includes but is not limited to polyethylene glycol 400.

[0112] In some of these embodiments, the protective agent includes but is not limited to trehalose and glycerol.

[0113] It should be noted that the stirring speed for adding the thickener with a mass fraction of 0.1% - 2% to water and stirring evenly to obtain the first mixed solution is 150 - 250 rpm; preferably, the stirring speed is 200 rpm.

[0114] It should be noted that an inducer with a mass fraction of 1% to 40% and a protective agent with a mass fraction of 1% to 10% are sequentially added into the first mixed solution, and the stirring temperature for uniformly stirring to obtain the second mixed solution is 40°C to 50°C, and the stirring speed is 100 to 200 rpm; preferably, the stirring temperature is 45°C and the stirring speed is 150 rpm.

[0115] It should be noted that silk fibroin with a mass fraction of 0.1% to 5% is added to the second mixed solution, and the stirring temperature for uniformly stirring to obtain the third mixed solution is 50°C to 60°C, and the stirring speed is 250 to 350 rpm; preferably, the stirring temperature is 55°C and the stirring speed is 300 rpm.

[0116] Furthermore, adding a thickener with a mass fraction of 0.1% to 2% into water and uniformly stirring to obtain the first mixed solution further includes adding one or more of dextran, hyaluronic acid, collagen, and chitosan into the mixture after adding the thickener with a mass fraction of 0.1% to 2% into water, and uniformly stirring to obtain the first mixed solution.

[0117] Example 15

[0118] This example relates to the application of the silk fibroin gel dressing in the fields of medical aesthetics, medical devices, and cosmetics in the present invention.

[0119] The silk fibroin gel dressing can achieve the care and repair of chronic and non-chronic wounds, as well as skin moisturizing, soothing, and anti-aging in products in the fields of medical aesthetics, medical devices, and cosmetics.

[0120] The technical features of the above-described embodiments can be combined arbitrarily. For the sake of concise description, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, it should be considered as the scope described in this specification.

[0121] The above-described embodiments only represent several implementation manners of the present application. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present application, several modifications and improvements can still be made, and these all belong to the protection scope of the present application. Therefore, the protection scope of the patent of the present application shall be subject to the appended claims.

Claims

1. A silk fibroin gel dressing, characterized in that: It includes a main formula of silk fibroin gel dressing, which includes water, silk fibroin, an inducer, a protective agent, and a thickener; The mass fraction of the silk fibroin is 0.1% to 5%, the mass fraction of the inducer is 1% to 40%, the mass fraction of the protective agent is 1% to 10%, the mass fraction of the thickener is 0.1% to 2%, and the balance is water.

2. The silk fibroin gel dressing according to claim 1, characterized in that: The inducing agent includes one or more of polyethylene glycol 300, polyethylene glycol 400, polyethylene glycol 600, polyethylene glycol 1000, glycerol, butylene glycol, propylene glycol, pentanediol, and hexylene glycol.

3. The silk fibroin gel dressing according to claim 1, characterized in that: The protective agent includes one or more of trehalose, glycerol and poloxamer.

4. The silk fibroin gel dressing according to claim 1, characterized in that: The thickener includes one or more of carbomer, carrageenan, sodium alginate, xanthan gum, and hydroxyethyl cellulose.

5. The silk fibroin gel dressing according to claim 4, characterized in that: When the thickener is carbomer, the main formula of the silk fibroin gel dressing further includes triethanolamine and / or sodium hydroxide.

6. The silk fibroin gel dressing according to claim 1, characterized in that: Also included is a silk fibroin gel dressing auxiliary formula, wherein the silk fibroin gel dressing auxiliary formula includes one or more of dextran, hyaluronic acid, collagen, and chitosan; The mass fraction of dextran is 0.01% to 10%, the mass fraction of hyaluronic acid is 0.1% to 1.5%, the mass fraction of collagen is 0.01% to 2%, and the mass fraction of chitosan is 0.1% to 3%.

7. A method for preparing a silk fibroin gel dressing, characterized in that: include: Adding 0.1% to 2% of a thickener by mass into water and stirring evenly to obtain a first mixed solution; Adding 1% to 40% of the inducer by mass and 1% to 10% of the protective agent by mass into the first mixed solution in sequence, and stirring evenly to obtain a second mixed solution; Adding 0.1% to 5% by mass of silk fibroin to the second mixed solution, and stirring evenly to obtain a third mixed solution; The third mixed solution is sterilized by wet heat sterilization or radiation sterilization to obtain a silk fibroin gel dressing.

8. The preparation method according to claim 7, characterized in that: The thickener having a mass fraction of 0.1% to 2% is added to water, and stirred evenly to obtain a first mixed solution at a stirring speed of 150 to 250 rpm; and / or The inducing agent with a mass fraction of 1% to 40% and the protective agent with a mass fraction of 1% to 10% are sequentially added to the first mixed solution, and stirred evenly to obtain a second mixed solution at a stirring temperature of 40° C. to 50° C. and a stirring speed of 100 to 200 rpm; and / or The silk fibroin with a mass fraction of 0.1% to 5% is added to the second mixed solution, and stirred evenly to obtain the third mixed solution. The stirring temperature is 50° C. to 60° C. and the stirring speed is 250 to 350 rpm.

9. The preparation method according to claim 7, characterized in that: The step of adding 0.1% to 2% of a thickener by mass into water and stirring the mixture to obtain a first mixed solution further comprises: After adding 0.1% to 2% of a thickener by mass into water, one or more of dextran, hyaluronic acid, collagen and chitosan are added thereto and stirred evenly to obtain a first mixed solution.

10. Application of a silk fibroin gel dressing according to any one of claims 1 to 6 in the fields of medical beauty, medical devices and cosmetics, characterized in that: It includes the care and repair of chronic and non-chronic wounds, as well as skin moisturizing, soothing and anti-aging.

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