Micro-fluidic chip

Abstract

The invention provides a micro-fluidic chip, relates to the technical field of micro-fluidic detection, and solves the technical problem that a centrifugal micro-fluidic chip in the prior art cannot directly observe whether the sample size in a sample adding groove is proper or not. The device comprises a chip substrate and a sealing membrane packaged on the upper surface of the substrate, a flat long groove is formed along the upper surface of the substrate, the flat long groove and the sealing membrane form a sample adding groove, a sample adding opening is formed in the sealing membrane, and the sample adding opening is communicated with the sample adding groove and used for injecting a sample into the sample adding groove; and a supporting device is arranged and is used for supporting the sealing membrane on the sample adding groove. By designing the structure of the sample adding groove, an operator can visually observe whether the sample adding process and the sample adding amount are appropriate or not.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic detection, and particularly to a microfluidic chip. Background Art

[0002] Microfluidics chip technology occupies a very important position in the field of biomedicine, especially in nucleic acid testing (NAT), by virtue of its unique advantages of miniaturization and high integration. The essence of this technology lies in its ability to integrate many complex processes such as sample pretreatment, mixing, chemical reaction, separation, and detection on one or more micro-sized chips, thus creating a micro laboratory. Microfluidic chips greatly reduce the requirements for sample and reagent usage, simplify the operation process, and significantly shorten the detection cycle. At the same time, they can effectively avoid various errors that may occur during the manual operation of traditional laboratories. For this reason, microfluidics technology has become the preferred technology in many fields such as chemical analysis, DNA sequencing, protein analysis, single-cell and single-molecule analysis, food safety monitoring, environmental monitoring, and drug screening. With the continuous in-depth research and the continuous development of technology, the application scope of microfluidic chips is constantly expanding, and its potential is huge. It is regarded by the industry as "a key technology that may have a major impact on the human lifestyle in the future".

[0003] Currently, the planar structure diagram of a microfluidic chip is as Figure 1 shown, which is divided into upper and lower layers. The upper layer is watertightly connected to the lower layer. A set of sample injection through-holes for sample addition is provided on the upper layer of the chip, and a sample addition groove for accommodating the sample injected through the through-holes is provided on the lower layer at the corresponding position of the sample injection through-holes. During centrifugation, the liquid sample enters the detection hole under the action of centrifugal force through various functional grooves and microfluidic channels of different shapes provided on the lower layer and undergoes optical detection. During this process, the sample needs to pass through a long channel and be separated multiple times. Only by injecting a sufficient amount of sample can it be ensured that the amount of sample finally entering the detection hole is sufficient for effective detection. Therefore, the existing sample addition grooves are made as large as possible in depth, and their capacity is generally larger than the sample amount required for one detection. In order to prevent sample overflow, the volume of the existing chip-designed sample addition grooves is much larger than the required sample volume. After sample addition, the sample falls to the bottom of the sample addition groove, and it is impossible to directly observe whether the sample addition amount is appropriate. Excessive or insufficient sample amount will affect the function of the microfluidic chip and thus affect the detection result. In view of this, there is an urgent need to develop a new type of microfluidic chip to solve the above-mentioned existing technical problems. Summary of the Invention

[0004] The purpose of the present invention is to provide a microfluidic chip to solve the related technical problems existing in the prior art. The many technical effects that can be produced by the preferred technical solutions among the many technical solutions provided by the present invention are described in detail below.

[0005] To achieve the above object, the present invention provides the following technical solutions: A microfluidic chip provided by the present invention includes: a chip substrate, on which a sample loading groove is provided for temporarily storing a sample; a sealing diaphragm, covering the chip substrate, on which a sample loading port is provided, and the sample loading port is communicated with the sample loading groove for injecting the sample into the sample loading groove; a plurality of detection holes, uniformly arranged along the circumferential direction of the chip substrate for accommodating the sample to be detected; a microfluidic channel for communicating the sample loading groove and the detection holes; the sample loading groove extends along the upper surface of the chip substrate and is in an overall flat and long groove shape; the substrate and the sealing diaphragm form the sample loading groove.

[0006] According to an embodiment of the present invention, a support member is provided. The support assembly includes a support ring installed on the chip substrate for supporting the sealing diaphragm. The outer circumferential side wall of the support ring is fixedly connected to the side wall of the sample loading groove. A through cavity is provided through the center of the support ring. The sample loading port is communicated with the upper end of the through cavity, and the lower end of the through cavity is communicated with the sample loading groove.

[0007] According to another embodiment of the present invention, a support member is provided. The support assembly includes a support ring installed on the chip substrate for supporting the sealing diaphragm. One end of the support ring abuts against the bottom of the sample loading groove and is fixedly connected to the chip substrate. The other end of the support ring abuts against the sealing diaphragm. A through cavity is provided through the center of the support ring. A notch communicating with the through cavity is provided at the bottom end of the support ring along a direction perpendicular to the through cavity. The sample loading port is communicated with the upper end of the through cavity, and the sample loading groove is communicated with the through cavity through the notch.

[0008] According to still another embodiment of the present invention, a support member is provided. The support assembly includes a support ring installed on the chip substrate for supporting the sealing diaphragm. One end of the support ring abuts against the bottom of the sample loading groove and is fixedly connected to the chip substrate. The other end of the support ring abuts against the sealing diaphragm. The support ring is in a semi-circular ring shape. A through cavity is provided through the center of the support ring. A notch communicating with the through cavity is provided on the circumferential side surface of the support ring along the direction of the through cavity. The sample loading port is communicated with the upper end of the through cavity, and the sample loading groove is communicated with the through cavity through the notch.

[0009] Further, the notch expands radially outwards, that is, the distance of the inner port of the notch is smaller than the distance of the outer port of the notch.

[0010] Preferably, the top surface of the support ring is fixedly connected to the sealing diaphragm.

[0011] According to still another embodiment of the present invention, the sample loading groove extends in an arc shape with the sample loading port as the starting point, and the sample loading groove is arranged along the circumferential direction of the chip substrate.

[0012] Further, the support assembly further includes a plurality of support sheets uniformly arranged along the extension direction of the sample addition groove. One end of each support sheet is fixedly connected to the chip substrate, and the other end of each support sheet is fixedly connected to the sealing diaphragm.

[0013] Further, the support assembly further includes a support sheet arranged along the extension direction of the sample addition groove. The support sheet is arc-shaped and located at the middle of the sample addition groove.

[0014] According to another embodiment of the present invention, a quantitative line is provided on the sealing diaphragm for marking the amount of sample added.

[0015] The following are the main technical effects of the present invention: By providing a sample addition port and a flat groove arc-shaped sample addition groove on the sealing diaphragm, the operator can intuitively observe whether the process and amount of sample addition are appropriate, thereby ensuring that an appropriate amount of sample can be injected each time for detection, improving the success rate of detection and the accuracy of results.

[0016] By introducing a support assembly to support the sealing diaphragm, especially below the sample addition port, the deformation or collapse of the sealing diaphragm is effectively prevented, avoiding the problem of insufficient sample addition caused by the deformation of the thin film.

[0017] In summary, through the innovative improvement of the existing technology, the present invention solves the technical problems that are prone to occur in the sample addition process of the existing microfluidic chips, provides a new solution for the development of microfluidic chip technology, and is expected to significantly improve the efficiency and result accuracy of related projects in the field of biological detection. Description of the Drawings

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0019] Figure 1 is a schematic plan view of the sample addition structure of a centrifugal microfluidic chip in the prior art; Figure 2 is a schematic overall structure diagram provided by Embodiment 1 of the present invention; Figure 3 is a schematic internal structure diagram provided by Embodiment 1 of the present invention: Figure 4 is a schematic partial structure diagram provided by Embodiment 1 of the present invention; Figure 5 is a schematic partial structure diagram provided by Embodiment 2 of the present invention; Figure 6 It is a partial structural schematic diagram provided by Embodiment 3 of the present invention; Figure 7 It is a partial structural schematic diagram provided by Embodiment 4 of the present invention; Figure 8 It is a partial structural schematic diagram provided by Embodiment 5 of the present invention; Figure 9 It is a partial structural schematic diagram provided by Embodiment 6 of the present invention; Figure 10 It is a schematic diagram of the effect after the sample adding groove of the present invention is improved compared with the prior art; Explanation of reference numerals: 100, chip substrate; 110, sealing diaphragm; 120, detection hole; 130, sample adding groove; 140, microfluidic channel; 150, sample adding port; 200, support assembly; 210, support ring; 220, through cavity; 230, notch; 240, support sheet; 250, quantitative line. Detailed implementation manners

[0020] To make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other implementation manners obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope protected by the present invention.

[0021] In the description of the present invention, it should be noted that unless otherwise specified, the meaning of "a plurality of" is two or more; the orientation or positional relationships indicated by the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus cannot be construed as a limitation to the present invention. In addition, the terms "first", "second", "third", etc. are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0022] In the description of the present invention, it should also be noted that unless otherwise clearly specified and defined, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0023] The following is combined with the attached Figure 2 - 10To further elaborate on this application, an embodiment of this application discloses a microfluidic chip. It includes a chip substrate and a sealing diaphragm encapsulated on the upper layer of the substrate. Various functional grooves and microfluidic channels of different shapes are formed on the substrate for placing and carrying the samples to be tested. To facilitate operation and improve space utilization, this chip substrate is preferably designed in a disc shape.

[0024] Specifically, as Figure 2 - 3 shown, a sample loading groove 130 is formed on the chip substrate 100 near the center of the substrate circle, and a plurality of detection holes 120 are formed on the chip substrate 100. The plurality of detection holes 120 are distributed near the outer edge of the chip substrate 100 and are evenly arranged along the circumferential direction of the chip substrate 100, ensuring that the samples (such as biological liquid samples like blood or urine) can be evenly distributed among the various detection points, improving the consistency and reliability of the detection.

[0025] A microfluidic channel 140 communicating the sample loading groove 130 with the plurality of detection holes 120 is also designed inside the chip substrate 100. The design of the microfluidic channel 140 not only ensures that the liquid sample can smoothly flow from the sample loading groove 130 to each detection hole 120, but also by adjusting the design of the microfluidic channel 140 (such as width, depth, and path), the flow characteristics and residence time of the sample can be flexibly adjusted according to different detection requirements, thereby optimizing the detection effect. In addition, the notch of the sample loading groove 130, the end of the detection hole 120, and the opening of the microfluidic channel 140 are all located on the top surface of the chip substrate 100, facilitating the operator to load samples and observe.

[0026] The sealing diaphragm 110, made of a transparent material, is adhesively fixed on the upper surface of the substrate 100 and forms a sample temporary storage area with the sample loading groove 130 on the substrate 100, maintaining the sealing and stability of the microfluidic chip. A sample loading port 150 is formed on the sealing diaphragm 110 and is communicated with the sample loading groove 130, allowing the operator to inject the sample to be tested into the sample loading groove 130 through this sample loading port 150. This design not only ensures the leak-free addition of the sample but also simplifies the operation steps and improves the work efficiency.

[0027] Among them, the sample loading groove 130 is integrally a flat and long groove, that is, its length and width extend along the upper surface of the chip substrate 100, its height extends along the chip thickness direction, and its width is greater than its height, and its cross-section is a rectangle or a quasi-rectangle with a length much greater than the width. In this way, a flat and long sample loading groove 130 is formed between the chip substrate 100 and the sealing diaphragm 110. After the sample is added to the sample loading groove 130, it contacts the surface of the substrate 100 and the sealing diaphragm 110. Under the action of capillary force, the sample can flow along the groove wall of the sample loading groove 130 and the surface of the sealing diaphragm 110 towards one end of the sample loading groove 130 away from the sample inlet 150 until the entire sample loading groove 130 is filled. In this way, the operator can intuitively see the amount of the added liquid sample. By precisely designing the capacity of the sample loading groove 130, it can be ensured that the added sample is as close as possible to the required sample amount for detection.

[0028] The material of the sealing diaphragm 110 can be plastic, glass, quartz, etc. In one embodiment, the sealing diaphragm 110 can be selected as a plastic film, which has a certain elasticity and can produce a certain degree of deformation without being torn under the action of appropriate external force. In this embodiment, the size of the sample inlet 150 is adaptively designed with the outer wall of the pipette tip. When the pipette tip is inserted into the sample inlet 150, the sample inlet 150 forms a tight fit with the outer wall of the pipette tip, thereby sealing the sample inlet 150 and sealing one end of the sample loading groove 130. During sample loading, under the action of liquid pressure, the sample flows towards the other end of the sample loading groove 130, so that the sample quickly fills the entire sample loading groove 130.

[0029] In addition, in order to make the sample fill the sample loading groove 130 more smoothly and prevent the sample from accumulating or even overflowing at the sample inlet 150, in one of the embodiments, the surfaces of the substrate 100 and the sealing diaphragm 110 on the inner wall of the sample loading groove 130 are subjected to a hydrophilic treatment, such as coating a hydrophilic agent on the surface of the substrate 100 or / and the surface of the sealing diaphragm 110.

[0030] In addition, in order to precisely control the added amount of the sample, the present application also provides a sample addition quantitative line 250. The quantitative line 250 can be set at the bottom of the sample loading groove 130 or on the sealing diaphragm 110 and is arranged along the extension direction of the fan shape. When the sample is added to the quantitative line 250, it indicates that the added sample amount has been sufficient to fill all the detection holes 120 without overflowing after passing through the microfluidic channel and the functional groove, reaching the ideal liquid amount required for sample detection. This design not only simplifies the operation process, avoids the situation of adding too much or too little sample, but also ensures the consistency and accuracy of each detection, greatly improving the reliability and repeatability of the detection results.

[0031] Figure 10 It is a schematic diagram for comparing the technical effects of the sample loading groove in the present application with the prior art. As Figure 10A. In the existing sample loading groove, it is relatively deep. When filling the sample for one - time detection to 3 / 4 of its depth, the operator cannot observe the sample volume situation, and it is impossible to visually observe whether the sample volume is sufficient. Figure 10 B. In the improved structure of this application, after the sample is added through the sample loading port with a pipette, the operator can visually observe the change in the sample loading volume from one side of the sealing diaphragm. For example, observe whether the sample loading volume reaches the quantitative line position to accurately master the sample loading volume.

[0032] Refer to Figure 3 and Figure 4 As shown in the figures, in an embodiment of this application, the sample loading groove 130 is designed to extend in an arc shape along the circumferential direction of the chip substrate 100, and its top - view presents a fan - shaped distribution. That is, the sample loading groove 130 has a first arc - shaped side wall close to the center of the substrate and a second arc - shaped side wall far from the center of the substrate. Among them, the second arc - shaped side wall gradually moves away from the center of the chip from one end of the sample loading groove 130 (the sample loading port end) to the other end, so that the sample loading groove 130 gradually moves away from the center of the substrate starting from the sample loading port. This unique design not only increases the physical capacity of the sample loading groove 130, enabling the sample loading groove 130 to more efficiently accommodate all the samples required during the detection process, but also enables all the samples in the sample loading groove 130 to enter the functional groove and micro - fluidic channel during the centrifugation process, reducing sample waste and improving the detection efficiency. In addition, the cross - sectional area of the sample loading groove 130 gradually increases from the sample loading end to the other end, which is conducive to the rapid inflow of the sample into the sample loading groove, thus avoiding the aggregation of the sample near the sample loading port during sample loading. In addition, such a design also makes it easier for the operator to observe the addition of the sample, enhancing the intuitiveness and convenience of the operation.

[0033] It should be noted that when the upper layer of the micro - fluidic chip is encapsulated with a thin - film material, during sample loading, the thin - film material is prone to deformation under the extrusion of the pipette. The actual capacity of the sample loading groove after deformation is less than the designed capacity, which is likely to cause insufficient sample volume. In view of the above deficiencies, the following embodiments will detail various specific implementation manners of this application.

[0034] Embodiment 1 Refer to Figure 3 and Figure 4 As shown in the figures, in an embodiment of this application, a support component 200 is further provided on the chip substrate 100. The support component 200 includes a support ring 210 fixedly installed on the chip substrate 100. The bottom end of the support ring 210 is fixedly connected to the bottom of the sample loading groove 130, and its top end is fixedly connected to the sealing diaphragm 110. Moreover, the support ring 210 and the sealing diaphragm 110 can be connected to each other by gluing to ensure the structural stability. And the support ring 210 is arranged at one end of the sample loading groove 130 far from the connection point of the micro - fluidic channel 140, avoiding interference with the micro - fluidic channel 140 during the sample loading process and ensuring the smoothness of the sample flow path.

[0035] A through cavity 220 is formed through the center of the support ring 210, and the top end of the through cavity 220 corresponds to and communicates with the sample addition port 150 on the sealing diaphragm 110. This allows a sample addition pipette or a sample containing tube to smoothly add a sample into the through cavity 220 through the sample addition port 150 and guide it into the sample addition groove 130. A notch 230 is provided on the side wall of the support ring 210 facing the sample addition groove 130 and is arranged in the height direction. The notch 230 communicates with the through cavity 220, that is, the support ring 210 is arranged in a semi-circular ring shape. Such a design not only provides a direct inflow path for the sample but also plays a guiding role during the sample addition process, preventing the sample from overflowing or splashing out, and ensuring the cleanliness and safety of the sample addition process.

[0036] The notch 230 expands radially outward, that is, the inner port of the notch 230 is smaller than the distance of the outer port of the notch 230. That is, when the four vertices of the notch 230 are connected to each other, it forms a trapezoidal shape, which facilitates the outward flow of the sample in the through cavity 220. That is, after the sample enters the through cavity 220, it can directly flow out from the notch 230 to the sample addition groove 130, avoiding the accumulation of the sample in the through cavity 220.

[0037] When a sample addition pipette or a sample containing tube injects a sample into the sample addition groove 130 through the sample addition port 150, the top end of the support ring 210 of the support ring will rigidly support the sample addition pipette or the sample containing tube, effectively supporting these devices and preventing the deformation or collapse of the sealing diaphragm 110 caused by uneven pressure. If the sealing diaphragm 110 is deformed or collapsed, it may cause unnecessary adhesion between the sample addition groove 130 and the sealing diaphragm 110, hindering the normal inflow of the sample and even causing the failure of the sample addition. Therefore, the design of the support ring 210 not only ensures the smooth progress of the sample addition process but also significantly improves the safety and reliability of the operation.

[0038] In addition, directly below the support ring 210, a groove is provided at the sample addition groove 130. The design of this groove further enhances the space utilization rate of the sample addition groove 130 and provides additional accommodation space for the sample. When the sample is injected into the sample addition groove 130 through the sample addition port 150 and the through cavity 220, the accommodation space of the groove can prevent the phenomenon of backflow and overflow of the added sample due to too fast speed and too much content.

[0039] The support assembly 200 can effectively support the sealing diaphragm 110, prevent the sealing diaphragm 100 from undergoing irreversible deformation under external force, avoid the problem of deformation or collapse of the sealing diaphragm 110 that may occur under external force during the liquid injection process, avoid causing the failure of the sample addition groove 130, and ensure the structural integrity and functional stability of the microfluidic chip during use.

[0040] Embodiment 2 The main difference between this embodiment and Embodiment 1 lies in the improved design of the support component 200, specifically manifested in the increased use of the support piece 240.

[0041] As shown in Figure 5 In this embodiment, in addition to the support ring 210 described in Embodiment 1, the support component 200 also includes a support piece 240 fixedly installed on the chip substrate 100. The support piece 240 is arranged along the extending direction of the arc of the sample loading groove 130, with one end close to the support ring 210 and the other end extending to the connection point of the sample loading groove 130 and the microfluidic channel 140. This enables the support piece 240 to cover the key area of the sample loading groove 130 and provide a wider support effect. The bottom end of the support piece 240 is fixedly connected to the bottom of the sample loading groove 130, and the top end is fixedly connected to the sealing diaphragm 110. The support piece 240 is arranged on the central dividing line of the sample loading groove 130, ensuring the symmetry and stability of the structure.

[0042] The added support piece 240 can provide additional support for the entire sealing diaphragm 110, effectively preventing deformation or collapse at the non-sample loading port 150 due to changes in the internal air pressure during the sample loading process. The combined design of the support piece 240 and the support ring 210 significantly enhances the structural strength of the entire support component 200. This enhancement not only improves the pressure resistance of the sample loading groove 130 but also guides the flow direction of the sample to a certain extent.

[0043] Embodiment 3 The main difference between this embodiment and Embodiment 2 lies in the improved design of the support component 200, specifically manifested in the reduced use of the support ring 210.

[0044] As shown in Figure 6 In this embodiment, the support component 200 only includes the support piece 240 arranged along the extending direction of the arc of the sample loading groove 130, with one end close to the support ring 210 and the other end extending to the connection point of the sample loading groove 130 and the microfluidic channel 140. This enables the support piece 240 to cover the key area of the sample loading groove 130 and provide a wider support effect. The bottom end of the support piece 240 is fixedly connected to the bottom of the sample loading groove 130, and the top end is fixedly connected to the sealing diaphragm 110. The support piece 240 is arranged on the central dividing line of the sample loading groove 130, ensuring the symmetry and stability of the structure.

[0045] By removing the support ring 210, the structure of the support component 200 becomes more concise. This not only reduces the manufacturing cost but also lowers the process difficulty and improves the production efficiency.

[0046] Even though the support ring 210 is removed, the design of the support piece 240 can still provide sufficient support for the sealing diaphragm 110. Both ends of the support piece 240 are respectively fixed on the bottom of the sampling groove 130 and the sealing diaphragm 110, ensuring the stability of the sealing diaphragm 110 when under pressure, preventing local deformation or collapse, and thus guaranteeing the stability and continuity of the sampling process.

[0047] The extended part of the support piece 240 can play a role in guiding the liquid flow, helping the sample reach the connection point of the microfluidic channel 140 faster, reducing the residence time of the sample in the sampling groove 130, and improving the response speed and efficiency of the entire system.

[0048] Example 4 The main difference between this example and Example 3 lies in the improved design of the support assembly 200, specifically manifested in the structural shape of the support piece 240.

[0049] Refer to Figure 7 As shown, the support assembly 200 includes a plurality of support pieces 240 for supporting the sealing diaphragm 110, and the plurality of support pieces 240 are uniformly arranged along the extending direction of the sampling groove 130. This ensures the uniform support of the sealing diaphragm 110 along the entire length of the sampling groove 130, avoiding deformation or damage caused by uneven local stress.

[0050] One end of each support piece 240 is fixedly connected to the chip substrate 100, that is, the bottom of the sampling groove 130, and the other end of the support piece 240 is fixedly connected to the sealing diaphragm 110. This fixing method ensures the stability of the support piece 240 and the flatness of the sealing diaphragm 110.

[0051] The support piece 240 is arranged in an arched shape, that is, there is a space at the lower end of the middle part of the support piece 240, providing a smooth flow path for the sample. This not only reduces the liquid level fluctuation but also ensures the uniform distribution of the sample in the sampling groove 130, improving the accuracy and consistency of the detection results.

[0052] Example 5 The main difference between this example and Example 1 lies in the improved design of the support assembly 200, specifically manifested in the structural shape of the support ring 210.

[0053] Refer to Figure 8 As shown, the support assembly 200 in this example includes a support ring 210 fixedly installed on the chip substrate 100. The outer circumferential side wall of the support ring 210 is fixedly connected to the side wall of the sampling groove 130, ensuring a firm connection between the support ring 210 and the sampling groove 130. There is a certain distance between the bottom end of the support ring 210 and the bottom of the sampling groove 130, while its top end is fixedly connected to the sealing diaphragm 110. This design not only ensures the structural stability but also provides space for the flow of the sample.

[0054] By fixedly connecting the outer circumferential side wall of the support ring 210 to the side wall of the sample loading groove 130, this fixing method ensures a firm connection between the support ring 210 and the sample loading groove 130. Even under external pressure or vibration, the support ring 210 can still remain stable, avoiding the risk of structural loosening or deformation.

[0055] A through cavity 220 is formed through the center of the support ring 210. The top end of the through cavity 220 corresponds to and communicates with the sample loading port 150 on the sealing diaphragm 110. The bottom end of the through cavity 220 also remains in communication with the bottom of the sample loading groove 130, so that the sample can be smoothly added into the through cavity 220 through the sample loading port 150, and then flow into the bottom of the sample loading groove 130 from the bottom end of the through cavity 220 and flow along the extending direction of the sample loading groove 130.

[0056] The central through cavity 220 of the support ring 210 provides a clear flow path for the sample. The sample enters the through cavity 220 through the sample loading port 150, and then flows into the bottom of the sample loading groove 130 from the bottom end of the through cavity 220 and flows smoothly along the extending direction of the sample loading groove 130. This design reduces the liquid level fluctuation, ensures the uniform distribution of the sample, and improves the accuracy of the detection result.

[0057] Example 6 The main difference between this embodiment and Embodiment 1 lies in the improved design of the support assembly 200, specifically manifested in the structural shape of the support ring 210.

[0058] Refer to Figure 9 As shown, the support assembly 200 in this embodiment includes a support ring 210 fixedly installed on the chip substrate 100. The bottom end of the support ring 210 is fixedly connected to the bottom of the sample loading groove 130, and its top end is fixedly connected to the sealing diaphragm 110, ensuring the structural stability. And the support ring 210 is arranged at one end of the sample loading groove 130 away from the connection point of the microfluidic channel 140, avoiding interference with the microfluidic channel 140 during the sample loading process and ensuring the unobstructed flow path of the sample.

[0059] A through cavity 220 is formed through the center of the support ring 210. The top end of the through cavity 220 corresponds to and communicates with the sample loading port 150 on the sealing diaphragm 110. This design allows a pipette or a liquid sample holding tube to smoothly add the sample into the through cavity 220 through the sample loading port 150 and guide it into the sample loading groove 130.

[0060] The side wall of the support ring 210 facing the sample loading groove 130 is provided with a notch 230 communicating with the through cavity 220. The notch 230 is arranged perpendicular to the direction of the through cavity 220, and the notch 230 is also opened at the bottom end of the support ring 210 to communicate with the sample loading groove 130. When a pipette or a liquid sample containing tube injects a sample into the sample loading groove 130 through the sample loading port 150, the sample first passes through the through cavity 220 and then flows out through the notch 230 and into the sample loading groove 130.

[0061] The central through cavity 220 of the support ring 210 is designed such that the sample can smoothly flow from the sample loading port 150 into the through cavity 220 and then flow out from the notch 230 on the side wall into the sample loading groove 130. This perpendicular arrangement of the through cavity 220 and the notch 230 can reduce the turbulence and vortices generated during the liquid flow. When the sample enters the notch 230 from the through cavity 220, the vertical flow reduces the lateral disturbance, making the liquid flow more smoothly, reducing the formation of bubbles, contributing to the accurate judgment of the sample volume addition result, and avoiding the interference of bubbles.

[0062] In summary, through the carefully designed support assembly 200, the present invention not only solves various problems that may occur during the sample loading process, such as the deformation of the sealing diaphragm 110 and the overflow of the sample, but also improves the accuracy and stability of the sample injection, laying a solid foundation for the realization of efficient and reliable biological sample detection.

[0063] The above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claimed rights.

Claims

1. A microfluidic chip, characterized in that: include: A chip substrate (100) is provided with a sample loading slot (130) for temporarily storing samples; A sealing membrane (110) is covered on the chip substrate (100) and is provided with a sample loading port (150), wherein the sample loading port (150) is in communication with the sample loading groove (130) and is used for injecting a sample into the sample loading groove (130); A plurality of detection holes (120) are evenly arranged along the circumferential direction of the chip substrate (100) and are used to accommodate samples to be detected; A microfluidic channel (140) for connecting the sample loading slot (130) and the detection hole (120); The sample loading groove (130) is extended and opened along the upper surface of the chip substrate (100), and is in the shape of an elongated groove as a whole; the substrate (100) and the sealing film (110) constitute the sample loading groove (130).

2. A microfluidic chip according to claim 1, characterized in that: A support component (200) is provided, the support component (200) comprising a support ring (210) mounted on a chip substrate (100) for supporting a sealing membrane (110), the outer circumferential side wall of the support ring (210) being fixedly connected to the side wall of a sample loading groove (130), a through cavity (220) being provided through the center of the support ring (210), the sample loading port (150) being in communication with the upper end of the through cavity (220), and the lower end of the through cavity (220) being in communication with the sample loading groove (130).

3. A microfluidic chip according to claim 1, characterized in that: A support component (200) is provided. The support component (200) comprises a support ring (210) mounted on the chip substrate (100) for supporting the sealing membrane (110). One end of the support ring (210) abuts against the bottom of the sample loading groove (130) and is fixedly connected to the chip substrate (100). The other end of the support ring (210) abuts against the sealing membrane (110). A through cavity (220) is provided through the center of the support ring (210). A notch (230) communicating with the through cavity (220) is provided at the bottom end of the support ring (210) in a direction perpendicular to the through cavity (220). The sample loading port (150) is communicated with the upper end of the through cavity (220). The sample loading groove (130) is communicated with the through cavity (220) through the notch (230).

4. A microfluidic chip according to claim 1, characterized in that: A support component (200) is provided. The support component (200) comprises a support ring (210) mounted on the chip substrate (100) for supporting the sealing membrane (110). One end of the support ring (210) abuts against the bottom of the sample loading groove (130) and is fixedly connected to the chip substrate (100). The other end of the support ring (210) abuts against the sealing membrane (110). The support ring (210) is arranged in a semicircular ring. A through cavity (220) is provided through the center of the support ring (210). A notch (230) communicating with the through cavity (220) is provided on the circumferential side surface of the support ring (210) along the through direction of the through cavity (220). The sample loading port (150) is communicated with the upper end of the through cavity (220). The sample loading groove (130) is communicated with the through cavity (220) through the notch (230).

5. A microfluidic chip according to claim 4, characterized in that: The notch (230) expands radially outward, and the distance between ports inside the notch (230) is smaller than the distance between ports outside the notch (230).

6. A microfluidic chip according to any one of claims 2 to 4, characterized in that: The top surface of the support ring (210) and the sealing diaphragm (110) are fixedly connected to each other.

7. A microfluidic chip according to claim 6, characterized in that: The support assembly (200) further comprises a plurality of support sheets (240) evenly arranged along the extension direction of the sample loading slot (130), one end of the support sheet (240) being fixedly connected to the chip substrate (100), and the other end of the support sheet (240) being fixedly connected to the sealing membrane (110).

8. The microfluidic chip according to claim 6, characterized in that: The support assembly (200) further comprises a support sheet (240) arranged along the extension direction of the sample loading slot (130); the support sheet (240) is arranged in an arc shape and is located in the middle of the sample loading slot (130).

9. The microfluidic chip according to claim 1, characterized in that: The sample loading groove (130) is extended in an arc shape with the sample loading port (150) as a starting point, and the sample loading groove (130) is arranged along the circumferential direction of the chip substrate (100).

10. The microfluidic chip according to claim 1, characterized in that: The sealing membrane (110) is provided with a quantitative line (250) for indicating the amount of sample added.

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